AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064977

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064977.t1 MTR_1g046680 98.361 244 4 0 1 244 1 244 0.0 499
MS.gene064977.t1 MTR_5g007600 71.915 235 51 3 1 235 1 220 4.40e-106 310
MS.gene064977.t1 MTR_5g007600 76.147 109 26 0 136 244 223 331 9.52e-57 184
MS.gene064977.t1 MTR_8g013950 47.340 188 84 7 63 241 114 295 1.13e-38 137
MS.gene064977.t1 MTR_8g076340 72.000 75 21 0 170 244 216 290 5.15e-36 129
MS.gene064977.t1 MTR_3g109760 44.279 201 71 7 63 241 95 276 8.80e-36 129
MS.gene064977.t1 MTR_2g094600 46.296 162 55 4 98 236 128 280 9.58e-36 129
MS.gene064977.t1 MTR_1g006650 78.571 70 15 0 172 241 234 303 8.98e-35 127
MS.gene064977.t1 MTR_3g117380 36.538 260 124 8 6 241 56 298 1.10e-34 126
MS.gene064977.t1 MTR_4g071590 50.000 140 44 5 124 239 141 278 2.55e-33 122
MS.gene064977.t1 MTR_4g471590 50.000 140 44 5 124 239 141 278 2.55e-33 122
MS.gene064977.t1 MTR_5g021340 71.233 73 21 0 172 244 300 372 3.38e-33 124
MS.gene064977.t1 MTR_6g032830 76.471 68 16 0 171 238 191 258 7.88e-33 120
MS.gene064977.t1 MTR_1g094930 70.130 77 21 1 160 236 137 211 7.96e-33 119
MS.gene064977.t1 MTR_7g085410 74.648 71 18 0 166 236 182 252 8.53e-33 120
MS.gene064977.t1 MTR_1g094930 70.130 77 21 1 160 236 232 306 3.30e-32 120
MS.gene064977.t1 MTR_4g058910 74.627 67 17 0 172 238 314 380 1.18e-31 120
MS.gene064977.t1 MTR_1g116010 71.642 67 19 0 170 236 162 228 1.86e-31 116
MS.gene064977.t1 MTR_7g446110 55.224 67 28 1 172 238 187 251 4.87e-19 83.6
MS.gene064977.t1 MTR_7g445980 54.286 70 29 2 170 238 31 98 6.71e-18 76.6
MS.gene064977.t1 MTR_7g445950 47.126 87 44 2 149 233 95 181 1.25e-17 78.2
MS.gene064977.t1 MTR_7g446070 46.739 92 45 3 149 238 107 196 2.38e-17 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064977.t1 AT3G24050 80.000 75 15 0 170 244 192 266 2.39e-40 140
MS.gene064977.t1 AT4G32890 38.909 275 100 10 7 241 20 266 3.97e-38 135
MS.gene064977.t1 AT4G36240 38.340 253 104 6 6 239 12 231 2.56e-36 129
MS.gene064977.t1 AT4G34680 46.479 142 63 3 111 242 112 250 4.98e-35 126
MS.gene064977.t1 AT4G34680 46.479 142 63 3 111 242 112 250 4.98e-35 126
MS.gene064977.t1 AT5G66320 79.412 68 14 0 172 239 249 316 9.00e-35 127
MS.gene064977.t1 AT5G66320 79.412 68 14 0 172 239 249 316 9.00e-35 127
MS.gene064977.t1 AT5G25830 77.143 70 16 0 172 241 219 288 1.22e-33 124
MS.gene064977.t1 AT3G51080 72.603 73 20 0 172 244 221 293 2.80e-33 122
MS.gene064977.t1 AT3G60530 75.362 69 17 0 171 239 157 225 2.91e-33 120
MS.gene064977.t1 AT1G08010 72.464 69 19 0 170 238 218 286 2.62e-32 120
MS.gene064977.t1 AT1G08010 72.464 69 19 0 170 238 218 286 2.62e-32 120
MS.gene064977.t1 AT1G08010 72.464 69 19 0 170 238 218 286 2.62e-32 120
MS.gene064977.t1 AT2G45050 75.000 68 17 0 172 239 179 246 3.81e-32 118
MS.gene064977.t1 AT1G08000 41.935 186 64 6 92 238 104 284 5.07e-32 119
MS.gene064977.t1 AT1G08000 41.935 186 64 6 92 238 104 284 5.07e-32 119
MS.gene064977.t1 AT3G54810 75.000 68 17 0 172 239 229 296 6.73e-31 116
MS.gene064977.t1 AT3G54810 75.000 68 17 0 172 239 229 296 6.73e-31 116
MS.gene064977.t1 AT3G45170 43.165 139 63 3 101 239 60 182 2.30e-27 104
MS.gene064977.t1 AT2G28340 66.667 63 21 0 174 236 193 255 1.02e-25 102

Find 83 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGAAGGAAAGGCTTTCTT+AGG 0.280723 1.4:+31252517 None:intergenic
GTCCAACAAAGATGCATTTC+CGG 0.285678 1.4:-31251900 MS.gene064977:CDS
GACATTTCCTCCCAATGGTT+GGG 0.293078 1.4:+31251600 None:intergenic
CTATTTGCAGGACGGTATTC+AGG 0.302785 1.4:+31251473 None:intergenic
CGAAAAGTTGGGCTATTTGC+AGG 0.311001 1.4:+31251461 None:intergenic
CATGCATTACAAAGTGTCTT+TGG 0.315428 1.4:+31251527 None:intergenic
CAAAGTGTCTTTGGACCATT+TGG 0.337970 1.4:+31251536 None:intergenic
GAAGGTGGATCACTGCATTC+TGG 0.356189 1.4:+31252490 None:intergenic
GTTGTTTCCACGGGAATTGT+AGG 0.366200 1.4:+31251660 None:intergenic
TGGTTCTGTGGACGACCTCT+TGG 0.388206 1.4:-31252581 MS.gene064977:CDS
GATGAGGAAGCAGAAGGGAA+TGG 0.399465 1.4:-31251399 MS.gene064977:CDS
TTTGTAATGCATGTGGGGTT+AGG 0.415099 1.4:-31251517 MS.gene064977:CDS
ACTTACAGAAAGTGAATTGA+TGG 0.418526 1.4:+31252441 None:intergenic
CCACGGGAATTGTAGGTTTG+AGG 0.425374 1.4:+31251667 None:intergenic
AAGCAGAAGGGAATGGGAAC+GGG 0.428545 1.4:-31251392 MS.gene064977:CDS
TGTGATTGTAGCCACTTAAG+AGG 0.429240 1.4:+31251753 None:intergenic
TGAATTGTCGTGTATTCAAC+CGG 0.432782 1.4:-31251861 MS.gene064977:CDS
AGCCGGAAATGCATCTTTGT+TGG 0.438765 1.4:+31251898 None:intergenic
TGCATGTCACTGCGAAAAGT+TGG 0.440834 1.4:+31251449 None:intergenic
TTGTGCTGTTCTCGAGCAGT+GGG 0.447298 1.4:+31251813 None:intergenic
ACATTTCCTCCCAATGGTTG+GGG 0.449081 1.4:+31251601 None:intergenic
ATTTGATGCTTCAAAAGATC+CGG 0.450692 1.4:+31251842 None:intergenic
GACTTGCTCCGTGCACGGAC+AGG 0.458205 1.4:+31251722 None:intergenic
TCAACAAATGTTTCAACAGC+CGG 0.462728 1.4:+31251881 None:intergenic
TTCGCTCAATCCATTGGCCC+TGG 0.469600 1.4:-31252470 MS.gene064977:CDS
CTTGTGCTGTTCTCGAGCAG+TGG 0.473765 1.4:+31251812 None:intergenic
CCAGGAGGAGGTTGCAGAAG+AGG 0.477241 1.4:-31251936 MS.gene064977:intron
GTGGAGATGAGGAAGCAGAA+GGG 0.478182 1.4:-31251404 MS.gene064977:CDS
TTGCAGAAGAGGAGCTTGAA+TGG 0.482772 1.4:-31251925 MS.gene064977:CDS
ATGAGGAAGCAGAAGGGAAT+GGG 0.486460 1.4:-31251398 MS.gene064977:CDS
GTGAAACAAACCCCAACCAT+TGG 0.493226 1.4:-31251611 MS.gene064977:CDS
TTGGTACTCGGTTGTTTCCA+CGG 0.494137 1.4:+31251650 None:intergenic
AACAGCATTACCCTCTTAAG+TGG 0.500575 1.4:-31251764 MS.gene064977:CDS
GCATGTCACTGCGAAAAGTT+GGG 0.502991 1.4:+31251450 None:intergenic
TGGTACTCGGTTGTTTCCAC+GGG 0.505676 1.4:+31251651 None:intergenic
CAATGGCGTGCAGGTCCAAA+TGG 0.508669 1.4:-31251551 MS.gene064977:CDS
TTGGATTTCTCATCTGACAT+AGG 0.508816 1.4:-31252562 MS.gene064977:CDS
AGTGGAGATGAGGAAGCAGA+AGG 0.514467 1.4:-31251405 MS.gene064977:CDS
TTCTGGTTTGAGTGAAGGAA+AGG 0.515866 1.4:+31252507 None:intergenic
TGACATTTCCTCCCAATGGT+TGG 0.516568 1.4:+31251599 None:intergenic
TGTGGGGTTAGGTATAAGTC+TGG 0.520333 1.4:-31251506 MS.gene064977:CDS
TCCACCTTCGCTCAATCCAT+TGG 0.521499 1.4:-31252476 MS.gene064977:CDS
GATGGAGGCCCTTGGTTCTG+TGG 0.522422 1.4:-31252593 MS.gene064977:CDS
TGAAACAAACCCCAACCATT+GGG 0.524385 1.4:-31251610 MS.gene064977:CDS
GTGAATTGATGGGATCATCC+AGG 0.528433 1.4:+31252452 None:intergenic
ATGGGATCATCCAGGGCCAA+TGG 0.530010 1.4:+31252460 None:intergenic
CTGCATTCTGGTTTGAGTGA+AGG 0.532294 1.4:+31252502 None:intergenic
TCATCTCCACTACTTTCCTG+TGG 0.533667 1.4:+31251417 None:intergenic
TCTCGTAGCAAGCCTCGCCT+TGG 0.534627 1.4:-31251701 MS.gene064977:CDS
GATACTCCACAATGGCGTGC+AGG 0.539263 1.4:-31251560 MS.gene064977:CDS
TTGAGGCATCAGCAAGGCCA+AGG 0.541565 1.4:+31251684 None:intergenic
GAAGCAGAAGGGAATGGGAA+CGG 0.544276 1.4:-31251393 MS.gene064977:CDS
GCCAATGGATTGAGCGAAGG+TGG 0.546210 1.4:+31252475 None:intergenic
ACTTAAGAGGGTAATGCTGT+TGG 0.550014 1.4:+31251766 None:intergenic
CATCTCCACTACTTTCCTGT+GGG 0.557649 1.4:+31251418 None:intergenic
TGAAGTTCCCTGTCCGTGCA+CGG 0.571256 1.4:-31251730 MS.gene064977:CDS
TTTGGACCTGCACGCCATTG+TGG 0.572595 1.4:+31251554 None:intergenic
ACTTGCTCCGTGCACGGACA+GGG 0.574860 1.4:+31251723 None:intergenic
TACGAGACTTGCTCCGTGCA+CGG 0.578328 1.4:+31251717 None:intergenic
ATGATGACATTTCCTCCCAA+TGG 0.580868 1.4:+31251595 None:intergenic
ACAGGAAAGTAGTGGAGATG+AGG 0.582547 1.4:-31251415 MS.gene064977:CDS
TGAATTGATGGGATCATCCA+GGG 0.600445 1.4:+31252453 None:intergenic
GCATCAGCAAGGCCAAGGCG+AGG 0.601336 1.4:+31251689 None:intergenic
GACACTTTGTAATGCATGTG+GGG 0.601729 1.4:-31251522 MS.gene064977:CDS
AAGACACTTTGTAATGCATG+TGG 0.602236 1.4:-31251524 MS.gene064977:CDS
TAATTCCCACAGGAAAGTAG+TGG 0.604924 1.4:-31251423 MS.gene064977:CDS
GAGGTCGTCCACAGAACCAA+GGG 0.608143 1.4:+31252585 None:intergenic
AGACACTTTGTAATGCATGT+GGG 0.608576 1.4:-31251523 MS.gene064977:CDS
CTTACAGAAAGTGAATTGAT+GGG 0.611737 1.4:+31252442 None:intergenic
GTGATTGTAGCCACTTAAGA+GGG 0.622729 1.4:+31251754 None:intergenic
ACATGCATTCTAATTCCCAC+AGG 0.630282 1.4:-31251433 MS.gene064977:CDS
AACAAACCCCAACCATTGGG+AGG 0.635541 1.4:-31251607 MS.gene064977:CDS
AGGGCCAATGGATTGAGCGA+AGG 0.639769 1.4:+31252472 None:intergenic
AGAGGTCGTCCACAGAACCA+AGG 0.642044 1.4:+31252584 None:intergenic
GGGAGGAAATGTCATCATTG+TGG 0.646984 1.4:-31251590 MS.gene064977:CDS
CCTCAAACCTACAATTCCCG+TGG 0.649034 1.4:-31251667 MS.gene064977:CDS
GACGGTATTCAGGTACAAGT+CGG 0.649278 1.4:+31251483 None:intergenic
AAGTTGGGCTATTTGCAGGA+CGG 0.658089 1.4:+31251465 None:intergenic
TTTCTCATCTGACATAGGTG+AGG 0.665571 1.4:-31252557 MS.gene064977:CDS
GTAGGTTTGAGGCATCAGCA+AGG 0.716777 1.4:+31251678 None:intergenic
TGTGCTGTTCTCGAGCAGTG+GGG 0.722420 1.4:+31251814 None:intergenic
TGTCAGATGAGAAATCCAAG+AGG 0.747403 1.4:+31252566 None:intergenic
GAGTTGATGATACTCCACAA+TGG 0.801331 1.4:-31251568 MS.gene064977:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTATTTTGAAAAGGTTAA+TGG + chr1.4:31251573-31251592 None:intergenic 20.0%
! AAGGAAAACAAGTAAAAATC+TGG + chr1.4:31251738-31251757 None:intergenic 25.0%
!!! ATCAAGCAAGTATTTTGAAA+AGG + chr1.4:31251580-31251599 None:intergenic 25.0%
!!! ATTCCTTTTTTCCATACTTA+TGG - chr1.4:31251984-31252003 MS.gene064977:intron 25.0%
!!! TCCTTTTAGAATCAATGTAT+TGG - chr1.4:31251753-31251772 MS.gene064977:CDS 25.0%
!!! TTCCTTTTTTCCATACTTAT+GGG - chr1.4:31251985-31252004 MS.gene064977:intron 25.0%
AACCCATAAGTATGGAAAAA+AGG + chr1.4:31251990-31252009 None:intergenic 30.0%
ACTTACAGAAAGTGAATTGA+TGG + chr1.4:31251542-31251561 None:intergenic 30.0%
ATTTGATGCTTCAAAAGATC+CGG + chr1.4:31252141-31252160 None:intergenic 30.0%
CTTACAGAAAGTGAATTGAT+GGG + chr1.4:31251541-31251560 None:intergenic 30.0%
TTCACTTTCTGTAAGTTTGA+CGG - chr1.4:31251546-31251565 MS.gene064977:CDS 30.0%
! AGAATCAATGTATTGGCATT+TGG - chr1.4:31251760-31251779 MS.gene064977:CDS 30.0%
! CACTTGCTAATATTCTACTT+TGG + chr1.4:31251712-31251731 None:intergenic 30.0%
!! GCCAATACATTGATTCTAAA+AGG + chr1.4:31251757-31251776 None:intergenic 30.0%
!!! CACTTTTTCTTTGGAAGTTT+TGG + chr1.4:31252352-31252371 None:intergenic 30.0%
!!! GGTTTGTTTCACTTTTTCTT+TGG + chr1.4:31252361-31252380 None:intergenic 30.0%
!!! TATCTAGTTTTGACTTGTTG+AGG - chr1.4:31251604-31251623 MS.gene064977:CDS 30.0%
AAGACACTTTGTAATGCATG+TGG - chr1.4:31252456-31252475 MS.gene064977:CDS 35.0%
AGACACTTTGTAATGCATGT+GGG - chr1.4:31252457-31252476 MS.gene064977:CDS 35.0%
CTTCACATAACCCATAAGTA+TGG + chr1.4:31251998-31252017 None:intergenic 35.0%
TACTTGTGATCACTGTTTCA+AGG - chr1.4:31251849-31251868 MS.gene064977:CDS 35.0%
TATTCACACTGAGAAAGATG+CGG + chr1.4:31251891-31251910 None:intergenic 35.0%
TCAACAAATGTTTCAACAGC+CGG + chr1.4:31252102-31252121 None:intergenic 35.0%
TCCTGGAACATGAAAATAAG+CGG + chr1.4:31252030-31252049 None:intergenic 35.0%
TGAATTGTCGTGTATTCAAC+CGG - chr1.4:31252119-31252138 MS.gene064977:intron 35.0%
TTGGATTTCTCATCTGACAT+AGG - chr1.4:31251418-31251437 MS.gene064977:CDS 35.0%
! CATGCATTACAAAGTGTCTT+TGG + chr1.4:31252456-31252475 None:intergenic 35.0%
!! TCTTTGGAAGTTTTGGTACT+CGG + chr1.4:31252345-31252364 None:intergenic 35.0%
AACAGCATTACCCTCTTAAG+TGG - chr1.4:31252216-31252235 MS.gene064977:intron 40.0%
ACATGCATTCTAATTCCCAC+AGG - chr1.4:31252547-31252566 MS.gene064977:CDS 40.0%
ACTTAAGAGGGTAATGCTGT+TGG + chr1.4:31252217-31252236 None:intergenic 40.0%
AGTGAAGGAAAGGCTTTCTT+AGG + chr1.4:31251466-31251485 None:intergenic 40.0%
ATGATGACATTTCCTCCCAA+TGG + chr1.4:31252388-31252407 None:intergenic 40.0%
GACACTTTGTAATGCATGTG+GGG - chr1.4:31252458-31252477 MS.gene064977:CDS 40.0%
GTCAACGACACAACAAACAT+TGG + chr1.4:31251927-31251946 None:intergenic 40.0%
GTCCAACAAAGATGCATTTC+CGG - chr1.4:31252080-31252099 MS.gene064977:intron 40.0%
GTGATTGTAGCCACTTAAGA+GGG + chr1.4:31252229-31252248 None:intergenic 40.0%
GTTTCAAGGTTTCCATGTCA+TGG - chr1.4:31251863-31251882 MS.gene064977:CDS 40.0%
TAATTCCCACAGGAAAGTAG+TGG - chr1.4:31252557-31252576 MS.gene064977:CDS 40.0%
TGAAACAAACCCCAACCATT+GGG - chr1.4:31252370-31252389 MS.gene064977:intron 40.0%
TGAATTGATGGGATCATCCA+GGG + chr1.4:31251530-31251549 None:intergenic 40.0%
TGTCAGATGAGAAATCCAAG+AGG + chr1.4:31251417-31251436 None:intergenic 40.0%
TGTGATTGTAGCCACTTAAG+AGG + chr1.4:31252230-31252249 None:intergenic 40.0%
TTTCTCATCTGACATAGGTG+AGG - chr1.4:31251423-31251442 MS.gene064977:CDS 40.0%
TTTGTAATGCATGTGGGGTT+AGG - chr1.4:31252463-31252482 MS.gene064977:CDS 40.0%
! CAAAGTGTCTTTGGACCATT+TGG + chr1.4:31252447-31252466 None:intergenic 40.0%
! TATTTTCATGTTCCAGGAGG+AGG - chr1.4:31252032-31252051 MS.gene064977:intron 40.0%
!! GAGTTGATGATACTCCACAA+TGG - chr1.4:31252412-31252431 MS.gene064977:intron 40.0%
!! GCTTATTTTCATGTTCCAGG+AGG - chr1.4:31252029-31252048 MS.gene064977:intron 40.0%
!! TTCTGGTTTGAGTGAAGGAA+AGG + chr1.4:31251476-31251495 None:intergenic 40.0%
AAAGATGCGGTTCCATGACA+TGG + chr1.4:31251878-31251897 None:intergenic 45.0%
AAGTTGGGCTATTTGCAGGA+CGG + chr1.4:31252518-31252537 None:intergenic 45.0%
ACATTTCCTCCCAATGGTTG+GGG + chr1.4:31252382-31252401 None:intergenic 45.0%
AGCCGGAAATGCATCTTTGT+TGG + chr1.4:31252085-31252104 None:intergenic 45.0%
ATGAGGAAGCAGAAGGGAAT+GGG - chr1.4:31252582-31252601 MS.gene064977:CDS 45.0%
CATCTCCACTACTTTCCTGT+GGG + chr1.4:31252565-31252584 None:intergenic 45.0%
CGAAAAGTTGGGCTATTTGC+AGG + chr1.4:31252522-31252541 None:intergenic 45.0%
CTATTTGCAGGACGGTATTC+AGG + chr1.4:31252510-31252529 None:intergenic 45.0%
GACATTTCCTCCCAATGGTT+GGG + chr1.4:31252383-31252402 None:intergenic 45.0%
GACGGTATTCAGGTACAAGT+CGG + chr1.4:31252500-31252519 None:intergenic 45.0%
GCATGTCACTGCGAAAAGTT+GGG + chr1.4:31252533-31252552 None:intergenic 45.0%
GGGAGGAAATGTCATCATTG+TGG - chr1.4:31252390-31252409 MS.gene064977:intron 45.0%
GTGAAACAAACCCCAACCAT+TGG - chr1.4:31252369-31252388 MS.gene064977:intron 45.0%
GTGAATTGATGGGATCATCC+AGG + chr1.4:31251531-31251550 None:intergenic 45.0%
TCATCTCCACTACTTTCCTG+TGG + chr1.4:31252566-31252585 None:intergenic 45.0%
TGACATTTCCTCCCAATGGT+TGG + chr1.4:31252384-31252403 None:intergenic 45.0%
TGCATGTCACTGCGAAAAGT+TGG + chr1.4:31252534-31252553 None:intergenic 45.0%
TGTGGGGTTAGGTATAAGTC+TGG - chr1.4:31252474-31252493 MS.gene064977:CDS 45.0%
TTGCAGAAGAGGAGCTTGAA+TGG - chr1.4:31252055-31252074 MS.gene064977:intron 45.0%
! ACAGGAAAGTAGTGGAGATG+AGG - chr1.4:31252565-31252584 MS.gene064977:CDS 45.0%
! GTTGTTTCCACGGGAATTGT+AGG + chr1.4:31252323-31252342 None:intergenic 45.0%
! TCGTTGACTAGTTGCATCAG+TGG - chr1.4:31251939-31251958 MS.gene064977:intron 45.0%
! TTGGTACTCGGTTGTTTCCA+CGG + chr1.4:31252333-31252352 None:intergenic 45.0%
!! CTGCATTCTGGTTTGAGTGA+AGG + chr1.4:31251481-31251500 None:intergenic 45.0%
!! GCCGCTTATTTTCATGTTCC+AGG - chr1.4:31252026-31252045 MS.gene064977:intron 45.0%
AACAAACCCCAACCATTGGG+AGG - chr1.4:31252373-31252392 MS.gene064977:intron 50.0%
AAGCAGAAGGGAATGGGAAC+GGG - chr1.4:31252588-31252607 MS.gene064977:CDS 50.0%
ACCGCAGGCACTAACAATCT+CGG + chr1.4:31251790-31251809 None:intergenic 50.0%
AGTGGAGATGAGGAAGCAGA+AGG - chr1.4:31252575-31252594 MS.gene064977:CDS 50.0%
CCACGGGAATTGTAGGTTTG+AGG + chr1.4:31252316-31252335 None:intergenic 50.0%
CCTCAAACCTACAATTCCCG+TGG - chr1.4:31252313-31252332 MS.gene064977:intron 50.0%
GAAGCAGAAGGGAATGGGAA+CGG - chr1.4:31252587-31252606 MS.gene064977:CDS 50.0%
GAAGGTGGATCACTGCATTC+TGG + chr1.4:31251493-31251512 None:intergenic 50.0%
GATGAGGAAGCAGAAGGGAA+TGG - chr1.4:31252581-31252600 MS.gene064977:CDS 50.0%
GTGGAGATGAGGAAGCAGAA+GGG - chr1.4:31252576-31252595 MS.gene064977:CDS 50.0%
TCCACCTTCGCTCAATCCAT+TGG - chr1.4:31251504-31251523 MS.gene064977:CDS 50.0%
TCCGAGATTGTTAGTGCCTG+CGG - chr1.4:31251786-31251805 MS.gene064977:CDS 50.0%
! GAATCTGTCATTTGCACCGC+AGG + chr1.4:31251805-31251824 None:intergenic 50.0%
! GTAGGTTTGAGGCATCAGCA+AGG + chr1.4:31252305-31252324 None:intergenic 50.0%
! TGGTACTCGGTTGTTTCCAC+GGG + chr1.4:31252332-31252351 None:intergenic 50.0%
! TTGTGCTGTTCTCGAGCAGT+GGG + chr1.4:31252170-31252189 None:intergenic 50.0%
AGAGGTCGTCCACAGAACCA+AGG + chr1.4:31251399-31251418 None:intergenic 55.0%
ATGGGATCATCCAGGGCCAA+TGG + chr1.4:31251523-31251542 None:intergenic 55.0%
CAATGGCGTGCAGGTCCAAA+TGG - chr1.4:31252429-31252448 MS.gene064977:intron 55.0%
GAGGTCGTCCACAGAACCAA+GGG + chr1.4:31251398-31251417 None:intergenic 55.0%
GATACTCCACAATGGCGTGC+AGG - chr1.4:31252420-31252439 MS.gene064977:intron 55.0%
TGAAGTTCCCTGTCCGTGCA+CGG - chr1.4:31252250-31252269 MS.gene064977:intron 55.0%
TTCGCTCAATCCATTGGCCC+TGG - chr1.4:31251510-31251529 MS.gene064977:CDS 55.0%
TTTGGACCTGCACGCCATTG+TGG + chr1.4:31252429-31252448 None:intergenic 55.0%
! CTTGTGCTGTTCTCGAGCAG+TGG + chr1.4:31252171-31252190 None:intergenic 55.0%
! TACGAGACTTGCTCCGTGCA+CGG + chr1.4:31252266-31252285 None:intergenic 55.0%
! TGGTTCTGTGGACGACCTCT+TGG - chr1.4:31251399-31251418 MS.gene064977:CDS 55.0%
! TGTGCTGTTCTCGAGCAGTG+GGG + chr1.4:31252169-31252188 None:intergenic 55.0%
! TTGAGGCATCAGCAAGGCCA+AGG + chr1.4:31252299-31252318 None:intergenic 55.0%
!! AGGGCCAATGGATTGAGCGA+AGG + chr1.4:31251511-31251530 None:intergenic 55.0%
!! GCCAATGGATTGAGCGAAGG+TGG + chr1.4:31251508-31251527 None:intergenic 55.0%
ACTTGCTCCGTGCACGGACA+GGG + chr1.4:31252260-31252279 None:intergenic 60.0%
CCAGGAGGAGGTTGCAGAAG+AGG - chr1.4:31252044-31252063 MS.gene064977:intron 60.0%
CCTCTTCTGCAACCTCCTCC+TGG + chr1.4:31252047-31252066 None:intergenic 60.0%
TCTCGTAGCAAGCCTCGCCT+TGG - chr1.4:31252279-31252298 MS.gene064977:intron 60.0%
GCATCAGCAAGGCCAAGGCG+AGG + chr1.4:31252294-31252313 None:intergenic 65.0%
! GACTTGCTCCGTGCACGGAC+AGG + chr1.4:31252261-31252280 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 31251388 31252614 31251388 ID=MS.gene064977
chr1.4 mRNA 31251388 31252614 31251388 ID=MS.gene064977.t1;Parent=MS.gene064977
chr1.4 exon 31252447 31252614 31252447 ID=MS.gene064977.t1.exon1;Parent=MS.gene064977.t1
chr1.4 CDS 31252447 31252614 31252447 ID=cds.MS.gene064977.t1;Parent=MS.gene064977.t1
chr1.4 exon 31251388 31251954 31251388 ID=MS.gene064977.t1.exon2;Parent=MS.gene064977.t1
chr1.4 CDS 31251388 31251954 31251388 ID=cds.MS.gene064977.t1;Parent=MS.gene064977.t1
Gene Sequence

>MS.gene064977

ATGGAGGCCCTTGGTTCTGTGGACGACCTCTTGGATTTCTCATCTGACATAGGTGAGGATGATGATGATGACAAACCTAAGAAAGCCTTTCCTTCACTCAAACCAGAATGCAGTGATCCACCTTCGCTCAATCCATTGGCCCTGGATGATCCCATCAATTCACTTTCTGTAAGTTTGACGGTCCATTAACCTTTTCAAAATACTTGCTTGATTTTTTATCTAGTTTTGACTTGTTGAGGTGTGATCATGAAAGCGAAAGCATGTGTGAATTATGATTTGTTTTGAATCAATTAGAATTTTGATATATTATATTGTTCTTGTCCAAAGTAGAATATTAGCAAGTGCTTCCAGATTTTTACTTGTTTTCCTTTTAGAATCAATGTATTGGCATTTGGAATTCCGAGATTGTTAGTGCCTGCGGTGCAAATGACAGATTCTCTGTTTGCATCAAAGTTTTCATTTACTTGTGATCACTGTTTCAAGGTTTCCATGTCATGGAACCGCATCTTTCTCAGTGTGAATACTAATTTCTTGATCCAATGTTTGTTGTGTCGTTGACTAGTTGCATCAGTGGAATGCGTGAGTGTACTTGAGAAATTCCTTTTTTCCATACTTATGGGTTATGTGAAGTTTAATGTGCCGCTTATTTTCATGTTCCAGGAGGAGGTTGCAGAAGAGGAGCTTGAATGGCTGTCCAACAAAGATGCATTTCCGGCTGTTGAAACATTTGTTGAATTGTCGTGTATTCAACCGGATCTTTTGAAGCATCAAATGACAGCCCCACTGCTCGAGAACAGCACAAGCAGCAGCAACAGCAATAACAGTTCCAACAGCATTACCCTCTTAAGTGGCTACAATCACATGAAGTTCCCTGTCCGTGCACGGAGCAAGTCTCGTAGCAAGCCTCGCCTTGGCCTTGCTGATGCCTCAAACCTACAATTCCCGTGGAAACAACCGAGTACCAAAACTTCCAAAGAAAAAGTGAAACAAACCCCAACCATTGGGAGGAAATGTCATCATTGTGGAGTTGATGATACTCCACAATGGCGTGCAGGTCCAAATGGTCCAAAGACACTTTGTAATGCATGTGGGGTTAGGTATAAGTCTGGCCGACTTGTACCTGAATACCGTCCTGCAAATAGCCCAACTTTTCGCAGTGACATGCATTCTAATTCCCACAGGAAAGTAGTGGAGATGAGGAAGCAGAAGGGAATGGGAACGGGTTGA
Protein sequence

>MS.gene064977.t1

MEALGSVDDLLDFSSDIGEDDDDDKPKKAFPSLKPECSDPPSLNPLALDDPINSLSEEVAEEELEWLSNKDAFPAVETFVELSCIQPDLLKHQMTAPLLENSTSSSNSNNSSNSITLLSGYNHMKFPVRARSKSRSKPRLGLADASNLQFPWKQPSTKTSKEKVKQTPTIGRKCHHCGVDDTPQWRAGPNGPKTLCNACGVRYKSGRLVPEYRPANSPTFRSDMHSNSHRKVVEMRKQKGMGTG