Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065015.t1 | XP_003601199.1 | 53.2 | 363 | 40 | 12 | 1 | 247 | 1 | 349 | 2.60E-78 | 302 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065015.t1 | Q8LPH6 | 50.4 | 133 | 13 | 1 | 1 | 80 | 1 | 133 | 8.0e-28 | 125.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065015.t1 | G7J4T2 | 53.2 | 363 | 40 | 12 | 1 | 247 | 1 | 349 | 1.9e-78 | 302.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene065015.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065015.t1 | MTR_3g077110 | 52.617 | 363 | 42 | 11 | 1 | 247 | 1 | 349 | 9.00e-104 | 305 |
| MS.gene065015.t1 | MTR_1g112760 | 54.762 | 252 | 25 | 7 | 3 | 190 | 4 | 230 | 9.36e-71 | 216 |
| MS.gene065015.t1 | MTR_4g105130 | 36.856 | 369 | 103 | 13 | 1 | 247 | 1 | 361 | 1.21e-51 | 172 |
| MS.gene065015.t1 | MTR_7g110830 | 45.139 | 144 | 26 | 1 | 1 | 91 | 1 | 144 | 1.27e-30 | 117 |
| MS.gene065015.t1 | MTR_1g085040 | 48.062 | 129 | 14 | 1 | 1 | 76 | 1 | 129 | 6.18e-30 | 115 |
| MS.gene065015.t1 | MTR_1g085640 | 42.384 | 151 | 30 | 2 | 1 | 94 | 1 | 151 | 9.93e-29 | 112 |
| MS.gene065015.t1 | MTR_7g111290 | 47.727 | 132 | 16 | 1 | 1 | 79 | 1 | 132 | 1.64e-24 | 100 |
| MS.gene065015.t1 | MTR_2g099740 | 40.800 | 125 | 21 | 1 | 1 | 72 | 1 | 125 | 7.27e-23 | 96.3 |
| MS.gene065015.t1 | MTR_8g017500 | 37.589 | 141 | 29 | 2 | 1 | 82 | 1 | 141 | 1.20e-22 | 95.1 |
| MS.gene065015.t1 | MTR_8g017440 | 40.323 | 124 | 21 | 1 | 1 | 71 | 1 | 124 | 1.73e-22 | 94.7 |
| MS.gene065015.t1 | MTR_8g017540 | 39.516 | 124 | 22 | 1 | 1 | 71 | 47 | 170 | 9.46e-22 | 93.2 |
| MS.gene065015.t1 | MTR_2g064160 | 39.683 | 126 | 23 | 1 | 4 | 76 | 3 | 128 | 3.90e-21 | 90.5 |
| MS.gene065015.t1 | MTR_6g009430 | 34.109 | 129 | 32 | 1 | 1 | 76 | 1 | 129 | 3.78e-20 | 88.2 |
| MS.gene065015.t1 | MTR_8g017350 | 39.200 | 125 | 22 | 2 | 1 | 71 | 1 | 125 | 1.24e-19 | 88.2 |
| MS.gene065015.t1 | MTR_8g017340 | 39.200 | 125 | 22 | 2 | 1 | 71 | 1 | 125 | 1.97e-19 | 87.0 |
| MS.gene065015.t1 | MTR_5g078140 | 37.984 | 129 | 27 | 1 | 1 | 76 | 1 | 129 | 2.42e-19 | 85.5 |
| MS.gene065015.t1 | MTR_4g478180 | 34.559 | 136 | 36 | 1 | 1 | 83 | 2 | 137 | 3.51e-19 | 85.5 |
| MS.gene065015.t1 | MTR_4g073420 | 34.109 | 129 | 32 | 1 | 1 | 76 | 1 | 129 | 5.39e-19 | 84.3 |
| MS.gene065015.t1 | MTR_8g095390 | 36.434 | 129 | 29 | 1 | 1 | 76 | 1 | 129 | 8.16e-19 | 84.0 |
| MS.gene065015.t1 | MTR_8g017480 | 71.111 | 45 | 13 | 0 | 1 | 45 | 1 | 45 | 8.57e-19 | 77.8 |
| MS.gene065015.t1 | MTR_3g065440 | 38.168 | 131 | 28 | 1 | 1 | 78 | 1 | 131 | 1.27e-18 | 83.6 |
| MS.gene065015.t1 | MTR_3g097450 | 29.012 | 162 | 54 | 2 | 1 | 109 | 1 | 154 | 2.43e-18 | 82.4 |
| MS.gene065015.t1 | MTR_7g011170 | 33.333 | 174 | 55 | 3 | 1 | 121 | 1 | 166 | 3.19e-18 | 82.4 |
| MS.gene065015.t1 | MTR_4g082230 | 35.156 | 128 | 30 | 1 | 1 | 75 | 1 | 128 | 3.37e-18 | 83.2 |
| MS.gene065015.t1 | MTR_4g102380 | 36.434 | 129 | 29 | 1 | 1 | 76 | 1 | 129 | 4.80e-18 | 81.3 |
| MS.gene065015.t1 | MTR_3g074520 | 35.156 | 128 | 30 | 1 | 1 | 75 | 1 | 128 | 6.61e-18 | 82.0 |
| MS.gene065015.t1 | MTR_8g017390 | 39.831 | 118 | 17 | 2 | 1 | 64 | 1 | 118 | 6.63e-18 | 82.8 |
| MS.gene065015.t1 | MTR_6g012690 | 35.938 | 128 | 29 | 1 | 1 | 75 | 1 | 128 | 7.58e-18 | 82.0 |
| MS.gene065015.t1 | MTR_8g098860 | 36.975 | 119 | 22 | 1 | 1 | 66 | 1 | 119 | 1.39e-17 | 80.9 |
| MS.gene065015.t1 | MTR_5g079670 | 32.374 | 139 | 36 | 2 | 3 | 83 | 2 | 140 | 1.75e-17 | 78.6 |
| MS.gene065015.t1 | MTR_4g082290 | 35.156 | 128 | 30 | 1 | 1 | 75 | 1 | 128 | 3.24e-17 | 80.5 |
| MS.gene065015.t1 | MTR_5g010020 | 42.353 | 85 | 47 | 1 | 1 | 83 | 1 | 85 | 1.62e-16 | 77.8 |
| MS.gene065015.t1 | MTR_3g461490 | 28.079 | 203 | 84 | 4 | 1 | 150 | 1 | 194 | 1.08e-15 | 75.5 |
| MS.gene065015.t1 | MTR_2g089620 | 32.061 | 131 | 36 | 1 | 1 | 78 | 1 | 131 | 1.13e-15 | 75.5 |
| MS.gene065015.t1 | MTR_5g007370 | 36.752 | 117 | 21 | 1 | 12 | 75 | 12 | 128 | 1.32e-15 | 75.1 |
| MS.gene065015.t1 | MTR_5g029840 | 32.812 | 128 | 33 | 1 | 1 | 75 | 1 | 128 | 2.08e-15 | 74.7 |
| MS.gene065015.t1 | MTR_2g011660 | 32.558 | 129 | 33 | 2 | 1 | 75 | 1 | 129 | 2.37e-15 | 74.7 |
| MS.gene065015.t1 | MTR_7g076740 | 35.385 | 130 | 30 | 2 | 1 | 76 | 1 | 130 | 5.38e-15 | 73.2 |
| MS.gene065015.t1 | MTR_3g083540 | 34.677 | 124 | 28 | 1 | 6 | 76 | 35 | 158 | 1.18e-14 | 72.4 |
| MS.gene065015.t1 | MTR_4g091490 | 31.783 | 129 | 34 | 2 | 1 | 75 | 1 | 129 | 1.55e-14 | 72.4 |
| MS.gene065015.t1 | MTR_4g485530 | 31.746 | 126 | 33 | 1 | 1 | 73 | 1 | 126 | 6.77e-14 | 68.6 |
| MS.gene065015.t1 | MTR_8g031360 | 34.351 | 131 | 31 | 3 | 1 | 76 | 1 | 131 | 6.28e-13 | 67.4 |
| MS.gene065015.t1 | MTR_4g065017 | 36.792 | 106 | 14 | 1 | 11 | 63 | 16 | 121 | 3.58e-12 | 65.5 |
| MS.gene065015.t1 | MTR_1g017140 | 25.185 | 270 | 110 | 9 | 1 | 181 | 1 | 267 | 4.43e-12 | 65.1 |
| MS.gene065015.t1 | MTR_3g028740 | 36.111 | 108 | 16 | 1 | 9 | 63 | 19 | 126 | 4.73e-12 | 65.1 |
| MS.gene065015.t1 | MTR_2g097910 | 36.190 | 105 | 14 | 1 | 12 | 63 | 20 | 124 | 8.54e-12 | 64.3 |
| MS.gene065015.t1 | MTR_5g042030 | 32.479 | 117 | 26 | 1 | 12 | 75 | 7 | 123 | 3.18e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065015.t1 | AT5G26660 | 50.376 | 133 | 13 | 1 | 1 | 80 | 1 | 133 | 2.28e-34 | 126 |
| MS.gene065015.t1 | AT1G57560 | 48.872 | 133 | 15 | 1 | 1 | 80 | 1 | 133 | 8.14e-32 | 118 |
| MS.gene065015.t1 | AT1G57560 | 49.612 | 129 | 12 | 1 | 1 | 76 | 1 | 129 | 9.60e-32 | 119 |
| MS.gene065015.t1 | AT1G09540 | 45.139 | 144 | 24 | 2 | 1 | 91 | 1 | 142 | 1.33e-29 | 114 |
| MS.gene065015.t1 | AT4G01680 | 44.755 | 143 | 25 | 2 | 1 | 90 | 1 | 142 | 3.57e-29 | 110 |
| MS.gene065015.t1 | AT4G01680 | 44.755 | 143 | 25 | 2 | 1 | 90 | 1 | 142 | 1.89e-28 | 110 |
| MS.gene065015.t1 | AT3G12720 | 39.837 | 123 | 21 | 1 | 4 | 73 | 14 | 136 | 1.74e-21 | 91.3 |
| MS.gene065015.t1 | AT3G13890 | 41.880 | 117 | 15 | 1 | 1 | 64 | 1 | 117 | 4.33e-21 | 90.9 |
| MS.gene065015.t1 | AT5G61420 | 37.209 | 129 | 28 | 1 | 1 | 76 | 1 | 129 | 1.44e-20 | 89.7 |
| MS.gene065015.t1 | AT1G66230 | 37.063 | 143 | 33 | 2 | 1 | 90 | 1 | 139 | 2.91e-20 | 87.8 |
| MS.gene065015.t1 | AT5G15310 | 31.795 | 195 | 67 | 4 | 1 | 137 | 1 | 187 | 3.67e-20 | 86.7 |
| MS.gene065015.t1 | AT5G15310 | 31.795 | 195 | 67 | 4 | 1 | 137 | 1 | 187 | 4.07e-20 | 87.8 |
| MS.gene065015.t1 | AT5G15310 | 31.795 | 195 | 67 | 4 | 1 | 137 | 1 | 187 | 4.83e-20 | 87.4 |
| MS.gene065015.t1 | AT1G34670 | 36.719 | 128 | 28 | 1 | 1 | 75 | 1 | 128 | 1.37e-19 | 87.0 |
| MS.gene065015.t1 | AT5G16600 | 34.932 | 146 | 42 | 1 | 1 | 93 | 1 | 146 | 1.91e-19 | 86.3 |
| MS.gene065015.t1 | AT4G01680 | 80.000 | 45 | 9 | 0 | 1 | 45 | 1 | 45 | 4.25e-19 | 85.5 |
| MS.gene065015.t1 | AT4G01680 | 45.238 | 84 | 43 | 3 | 8 | 90 | 73 | 154 | 3.66e-12 | 65.5 |
| MS.gene065015.t1 | AT4G22680 | 35.417 | 144 | 40 | 1 | 1 | 91 | 1 | 144 | 4.29e-19 | 84.3 |
| MS.gene065015.t1 | AT5G07690 | 35.659 | 129 | 30 | 1 | 1 | 76 | 1 | 129 | 8.65e-19 | 84.3 |
| MS.gene065015.t1 | AT4G34990 | 32.215 | 149 | 46 | 2 | 1 | 96 | 1 | 147 | 1.23e-18 | 83.2 |
| MS.gene065015.t1 | AT2G16720 | 31.293 | 147 | 46 | 2 | 1 | 94 | 1 | 145 | 1.68e-18 | 82.8 |
| MS.gene065015.t1 | AT1G63910 | 34.815 | 135 | 35 | 1 | 1 | 82 | 1 | 135 | 1.98e-18 | 83.6 |
| MS.gene065015.t1 | AT3G01140 | 32.240 | 183 | 60 | 4 | 1 | 126 | 1 | 176 | 3.62e-18 | 82.8 |
| MS.gene065015.t1 | AT4G21440 | 29.885 | 174 | 55 | 2 | 1 | 121 | 1 | 160 | 3.85e-18 | 82.8 |
| MS.gene065015.t1 | AT3G02940 | 33.594 | 128 | 32 | 1 | 1 | 75 | 1 | 128 | 4.09e-18 | 82.0 |
| MS.gene065015.t1 | AT3G02940 | 33.594 | 128 | 32 | 1 | 1 | 75 | 1 | 128 | 5.78e-18 | 82.0 |
| MS.gene065015.t1 | AT3G02940 | 33.594 | 128 | 32 | 1 | 1 | 75 | 1 | 128 | 5.78e-18 | 82.0 |
| MS.gene065015.t1 | AT5G60890 | 32.558 | 129 | 34 | 1 | 1 | 76 | 1 | 129 | 9.25e-18 | 80.9 |
| MS.gene065015.t1 | AT4G05100 | 34.109 | 129 | 31 | 2 | 1 | 75 | 1 | 129 | 2.14e-17 | 80.5 |
| MS.gene065015.t1 | AT4G12350 | 35.659 | 129 | 30 | 1 | 1 | 76 | 1 | 129 | 2.79e-17 | 79.7 |
| MS.gene065015.t1 | AT4G38620 | 29.730 | 148 | 48 | 2 | 1 | 95 | 1 | 145 | 5.35e-17 | 78.6 |
| MS.gene065015.t1 | AT4G09460 | 30.872 | 149 | 43 | 2 | 1 | 96 | 1 | 142 | 6.37e-17 | 77.8 |
| MS.gene065015.t1 | AT5G26660 | 52.703 | 74 | 33 | 2 | 8 | 80 | 30 | 102 | 7.23e-17 | 79.0 |
| MS.gene065015.t1 | AT5G07700 | 33.333 | 129 | 33 | 1 | 1 | 76 | 1 | 129 | 8.71e-17 | 79.0 |
| MS.gene065015.t1 | AT5G07700 | 33.333 | 129 | 33 | 1 | 1 | 76 | 1 | 129 | 8.71e-17 | 79.0 |
| MS.gene065015.t1 | AT3G01140 | 32.022 | 178 | 57 | 4 | 6 | 126 | 49 | 219 | 8.88e-17 | 79.3 |
| MS.gene065015.t1 | AT3G13890 | 64.815 | 54 | 18 | 1 | 1 | 54 | 1 | 53 | 1.14e-16 | 78.6 |
| MS.gene065015.t1 | AT1G22640 | 32.353 | 136 | 37 | 2 | 1 | 83 | 1 | 134 | 1.62e-16 | 77.0 |
| MS.gene065015.t1 | AT4G28110 | 32.031 | 128 | 34 | 1 | 1 | 75 | 1 | 128 | 1.71e-16 | 77.4 |
| MS.gene065015.t1 | AT5G16770 | 32.812 | 128 | 33 | 1 | 1 | 75 | 1 | 128 | 1.82e-16 | 77.8 |
| MS.gene065015.t1 | AT5G16770 | 32.812 | 128 | 33 | 1 | 1 | 75 | 1 | 128 | 1.82e-16 | 77.8 |
| MS.gene065015.t1 | AT5G14340 | 34.109 | 129 | 32 | 1 | 1 | 76 | 13 | 141 | 2.18e-16 | 77.0 |
| MS.gene065015.t1 | AT5G16770 | 32.812 | 128 | 33 | 1 | 1 | 75 | 1 | 128 | 2.50e-16 | 77.4 |
| MS.gene065015.t1 | AT5G16770 | 32.812 | 128 | 33 | 1 | 1 | 75 | 1 | 128 | 2.50e-16 | 77.4 |
| MS.gene065015.t1 | AT3G61250 | 31.319 | 182 | 62 | 5 | 1 | 129 | 1 | 172 | 2.68e-16 | 77.0 |
| MS.gene065015.t1 | AT3G13540 | 31.579 | 152 | 51 | 1 | 6 | 104 | 17 | 168 | 2.93e-16 | 76.3 |
| MS.gene065015.t1 | AT4G17785 | 34.307 | 137 | 36 | 2 | 1 | 83 | 1 | 137 | 5.08e-16 | 76.6 |
| MS.gene065015.t1 | AT4G17785 | 34.307 | 137 | 36 | 2 | 1 | 83 | 1 | 137 | 5.34e-16 | 76.6 |
| MS.gene065015.t1 | AT1G35515 | 32.308 | 130 | 35 | 1 | 1 | 77 | 1 | 130 | 6.12e-16 | 74.7 |
| MS.gene065015.t1 | AT1G18570 | 32.308 | 130 | 34 | 2 | 1 | 76 | 1 | 130 | 1.64e-15 | 75.1 |
| MS.gene065015.t1 | AT5G56110 | 32.558 | 129 | 34 | 1 | 1 | 76 | 1 | 129 | 2.03e-15 | 74.7 |
| MS.gene065015.t1 | AT5G10280 | 45.946 | 74 | 38 | 2 | 3 | 75 | 56 | 128 | 2.69e-15 | 74.7 |
| MS.gene065015.t1 | AT5G65230 | 34.091 | 132 | 34 | 1 | 1 | 79 | 1 | 132 | 2.74e-15 | 74.3 |
| MS.gene065015.t1 | AT1G74080 | 30.070 | 143 | 47 | 1 | 1 | 90 | 1 | 143 | 3.01e-15 | 74.3 |
| MS.gene065015.t1 | AT5G54230 | 32.824 | 131 | 35 | 1 | 1 | 78 | 1 | 131 | 1.11e-14 | 72.8 |
| MS.gene065015.t1 | AT3G28470 | 35.659 | 129 | 30 | 1 | 1 | 76 | 1 | 129 | 2.46e-14 | 71.6 |
| MS.gene065015.t1 | AT1G74430 | 34.375 | 128 | 31 | 1 | 1 | 75 | 1 | 128 | 5.99e-14 | 70.1 |
| MS.gene065015.t1 | AT1G18710 | 42.857 | 70 | 40 | 0 | 8 | 77 | 61 | 130 | 3.69e-13 | 67.8 |
| MS.gene065015.t1 | AT1G22640 | 42.857 | 77 | 40 | 3 | 8 | 83 | 4 | 77 | 9.50e-13 | 65.5 |
| MS.gene065015.t1 | AT5G62320 | 28.758 | 153 | 45 | 3 | 2 | 90 | 3 | 155 | 3.23e-12 | 64.7 |
| MS.gene065015.t1 | AT5G61420 | 68.293 | 41 | 12 | 1 | 37 | 76 | 10 | 50 | 6.91e-12 | 64.3 |
| MS.gene065015.t1 | AT5G15310 | 38.318 | 107 | 52 | 4 | 37 | 137 | 10 | 108 | 7.91e-12 | 63.9 |
| MS.gene065015.t1 | AT3G08500 | 24.506 | 253 | 112 | 7 | 4 | 198 | 19 | 250 | 1.23e-11 | 63.9 |
| MS.gene065015.t1 | AT3G48920 | 33.333 | 126 | 29 | 3 | 9 | 79 | 15 | 140 | 5.16e-11 | 61.6 |
| MS.gene065015.t1 | AT5G12870 | 34.862 | 109 | 18 | 1 | 10 | 65 | 16 | 124 | 8.72e-11 | 61.2 |
Find 38 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATACAGTTCATAATAAT+TGG | 0.135565 | 1.2:-83450893 | None:intergenic |
| AAGAGCTTCTTATCTTCTTC+AGG | 0.255579 | 1.2:-83449580 | None:intergenic |
| TCTTCTGACAGCGATGTTAA+AGG | 0.277255 | 1.2:-83450831 | None:intergenic |
| TGCGGTGGCATTATCATTGA+TGG | 0.307466 | 1.2:-83450426 | None:intergenic |
| TTCAATTACATAACCATGTT+TGG | 0.311883 | 1.2:+83449601 | MS.gene065015:CDS |
| TTAAGATTACCAGCTAGTTT+AGG | 0.344561 | 1.2:-83449646 | None:intergenic |
| AAATACAGTTCATAATAATT+GGG | 0.349576 | 1.2:-83450892 | None:intergenic |
| AAGAGATTCATTATTGATCA+AGG | 0.401722 | 1.2:-83450382 | None:intergenic |
| CATCAACAAGACAGATTCAA+TGG | 0.439637 | 1.2:+83450794 | MS.gene065015:CDS |
| ATCAAGGGTTTATGTGTAGC+TGG | 0.461421 | 1.2:-83450366 | None:intergenic |
| TTCACTTCAAACTGCAACAA+AGG | 0.467959 | 1.2:-83450747 | None:intergenic |
| TACATAACCATGTTTGGTGT+TGG | 0.474556 | 1.2:+83449607 | MS.gene065015:CDS |
| AAGGCATTGAAGAGAAGAAC+TGG | 0.481895 | 1.2:-83450626 | None:intergenic |
| AGAGATTCATTATTGATCAA+GGG | 0.507195 | 1.2:-83450381 | None:intergenic |
| CCATGTTTGGTGTTGGATGT+TGG | 0.512330 | 1.2:+83449614 | MS.gene065015:CDS |
| TGAAAATTACAATAAGCTGT+TGG | 0.514835 | 1.2:+83450722 | MS.gene065015:CDS |
| AGTTCAGTTCCTAAACTAGC+TGG | 0.517760 | 1.2:+83449637 | MS.gene065015:CDS |
| AGAGCTTCTTATCTTCTTCA+GGG | 0.522102 | 1.2:-83449579 | None:intergenic |
| TGTTCAAATTGTAATTGACT+TGG | 0.522549 | 1.2:-83450600 | None:intergenic |
| CAATAATGAATCTCTTGTAA+AGG | 0.536511 | 1.2:+83450389 | MS.gene065015:CDS |
| ATCTCTTGTAAAGGAAGAGA+AGG | 0.539524 | 1.2:+83450398 | MS.gene065015:CDS |
| ATGCTTAAATTGTAGTGCAA+TGG | 0.540486 | 1.2:-83450555 | None:intergenic |
| CCAACATCCAACACCAAACA+TGG | 0.541756 | 1.2:-83449614 | None:intergenic |
| TGATTAATGAAGATGCCTCA+TGG | 0.545480 | 1.2:+83450694 | MS.gene065015:CDS |
| AATGGATCAAAATCAAATGT+TGG | 0.545963 | 1.2:-83450537 | None:intergenic |
| AATTGTAATTGACTTGGCTG+AGG | 0.553273 | 1.2:-83450594 | None:intergenic |
| GATTAATGAAGATGCCTCAT+GGG | 0.562741 | 1.2:+83450695 | MS.gene065015:CDS |
| TGACAGCGATGTTAAAGGAT+AGG | 0.564100 | 1.2:-83450826 | None:intergenic |
| AATACAGTTCATAATAATTG+GGG | 0.584426 | 1.2:-83450891 | None:intergenic |
| AATGAAGATGCCTCATGGGA+AGG | 0.591934 | 1.2:+83450699 | MS.gene065015:CDS |
| TGCTTCTGAATATTCATGTG+AGG | 0.611193 | 1.2:-83450481 | None:intergenic |
| GAGCTTCTTATCTTCTTCAG+GGG | 0.611549 | 1.2:-83449578 | None:intergenic |
| CAATGATAATGCCACCGCAA+AGG | 0.614078 | 1.2:+83450430 | MS.gene065015:CDS |
| AAGAAGAAACTAATGAAACA+AGG | 0.624080 | 1.2:+83450336 | MS.gene065015:CDS |
| TGTGTAGCTAAAGTCCTTTG+CGG | 0.640465 | 1.2:-83450444 | None:intergenic |
| AGAAGAAACTAATGAAACAA+GGG | 0.641105 | 1.2:+83450337 | MS.gene065015:CDS |
| TCGCTTTCAATATTTAGACA+AGG | 0.651721 | 1.2:-83450645 | None:intergenic |
| GTAGCTAAAGTCCTTTGCGG+TGG | 0.663390 | 1.2:-83450441 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAATGAGATAAAAAACTTT+TGG | + | chr1.2:83450301-83450320 | MS.gene065015:CDS | 15.0% |
| !! | AAAAACTAAGAAAAGGTTTA+TGG | + | chr1.2:83449554-83449573 | MS.gene065015:CDS | 20.0% |
| !! | ATGGTAAAGGAAAAAATATA+AGG | - | chr1.2:83449995-83450014 | None:intergenic | 20.0% |
| !! | TCATAAAAATTATATACGCA+TGG | - | chr1.2:83450014-83450033 | None:intergenic | 20.0% |
| !! | TTAAAGCAAAAACTAAGAAA+AGG | + | chr1.2:83449547-83449566 | MS.gene065015:CDS | 20.0% |
| !!! | ATATAATTTTTATGAGTTGC+AGG | + | chr1.2:83450019-83450038 | MS.gene065015:intron | 20.0% |
| !!! | TATAATTTTTATGAGTTGCA+GGG | + | chr1.2:83450020-83450039 | MS.gene065015:intron | 20.0% |
| ! | AAATTATATACGCATGGTAA+AGG | - | chr1.2:83450008-83450027 | None:intergenic | 25.0% |
| ! | AAGAAGAAACTAATGAAACA+AGG | + | chr1.2:83450336-83450355 | MS.gene065015:CDS | 25.0% |
| ! | AAGAGATTCATTATTGATCA+AGG | - | chr1.2:83450385-83450404 | None:intergenic | 25.0% |
| ! | AATGGATCAAAATCAAATGT+TGG | - | chr1.2:83450540-83450559 | None:intergenic | 25.0% |
| ! | AGAAGAAACTAATGAAACAA+GGG | + | chr1.2:83450337-83450356 | MS.gene065015:CDS | 25.0% |
| ! | AGAGATTCATTATTGATCAA+GGG | - | chr1.2:83450384-83450403 | None:intergenic | 25.0% |
| ! | CAATAATGAATCTCTTGTAA+AGG | + | chr1.2:83450389-83450408 | MS.gene065015:CDS | 25.0% |
| ! | TGAAAATTACAATAAGCTGT+TGG | + | chr1.2:83450722-83450741 | MS.gene065015:CDS | 25.0% |
| ! | TGAGATGGATAAACTATTTA+AGG | + | chr1.2:83450071-83450090 | MS.gene065015:intron | 25.0% |
| ! | TGTTCAAATTGTAATTGACT+TGG | - | chr1.2:83450603-83450622 | None:intergenic | 25.0% |
| ! | TTCAATTACATAACCATGTT+TGG | + | chr1.2:83449601-83449620 | MS.gene065015:CDS | 25.0% |
| !!! | TAGTTTTTGCTTTAAACAAC+AGG | - | chr1.2:83449542-83449561 | None:intergenic | 25.0% |
| AAATTGCATCACAATTACCA+GGG | + | chr1.2:83450271-83450290 | MS.gene065015:intron | 30.0% | |
| ATAGTTGCTGTATATCTTGT+TGG | - | chr1.2:83449722-83449741 | None:intergenic | 30.0% | |
| ATGCTTAAATTGTAGTGCAA+TGG | - | chr1.2:83450558-83450577 | None:intergenic | 30.0% | |
| TAGTTGCTGTATATCTTGTT+GGG | - | chr1.2:83449721-83449740 | None:intergenic | 30.0% | |
| TCGCTTTCAATATTTAGACA+AGG | - | chr1.2:83450648-83450667 | None:intergenic | 30.0% | |
| TTAATTTCAAATGATGCCTC+AGG | + | chr1.2:83450241-83450260 | MS.gene065015:intron | 30.0% | |
| ! | TGAGAATATGATGAAAGTGT+GGG | - | chr1.2:83449808-83449827 | None:intergenic | 30.0% |
| ! | TTAAGATTACCAGCTAGTTT+AGG | - | chr1.2:83449649-83449668 | None:intergenic | 30.0% |
| ! | TTGGGTTGTACAAGTATATA+TGG | - | chr1.2:83449703-83449722 | None:intergenic | 30.0% |
| AAGAGCTTCTTATCTTCTTC+AGG | - | chr1.2:83449583-83449602 | None:intergenic | 35.0% | |
| AGAGCTTCTTATCTTCTTCA+GGG | - | chr1.2:83449582-83449601 | None:intergenic | 35.0% | |
| AGGAATGTTCTCAAAACAAG+AGG | + | chr1.2:83450108-83450127 | MS.gene065015:intron | 35.0% | |
| ATCTCTTGTAAAGGAAGAGA+AGG | + | chr1.2:83450398-83450417 | MS.gene065015:CDS | 35.0% | |
| ATGGCATTCATGCATGTTTA+TGG | - | chr1.2:83449684-83449703 | None:intergenic | 35.0% | |
| CAAATTGCATCACAATTACC+AGG | + | chr1.2:83450270-83450289 | MS.gene065015:intron | 35.0% | |
| CATCAACAAGACAGATTCAA+TGG | + | chr1.2:83450794-83450813 | MS.gene065015:CDS | 35.0% | |
| GATTAATGAAGATGCCTCAT+GGG | + | chr1.2:83450695-83450714 | MS.gene065015:CDS | 35.0% | |
| GTGAGAATATGATGAAAGTG+TGG | - | chr1.2:83449809-83449828 | None:intergenic | 35.0% | |
| TGATTAATGAAGATGCCTCA+TGG | + | chr1.2:83450694-83450713 | MS.gene065015:CDS | 35.0% | |
| TGCTTCTGAATATTCATGTG+AGG | - | chr1.2:83450484-83450503 | None:intergenic | 35.0% | |
| TTCACTTCAAACTGCAACAA+AGG | - | chr1.2:83450750-83450769 | None:intergenic | 35.0% | |
| ! | TGGATAAACTATTTAAGGCC+TGG | + | chr1.2:83450076-83450095 | MS.gene065015:intron | 35.0% |
| ! | TTCATGAAGCACTTGGAAAT+AGG | + | chr1.2:83450149-83450168 | MS.gene065015:intron | 35.0% |
| !! | AATTGTAATTGACTTGGCTG+AGG | - | chr1.2:83450597-83450616 | None:intergenic | 35.0% |
| !! | TACATAACCATGTTTGGTGT+TGG | + | chr1.2:83449607-83449626 | MS.gene065015:CDS | 35.0% |
| AACATTCCTCTCTTCAAACC+AGG | - | chr1.2:83450097-83450116 | None:intergenic | 40.0% | |
| AGTTCAGTTCCTAAACTAGC+TGG | + | chr1.2:83449637-83449656 | MS.gene065015:CDS | 40.0% | |
| ATCAAGGGTTTATGTGTAGC+TGG | - | chr1.2:83450369-83450388 | None:intergenic | 40.0% | |
| ATCTCATTATCTGTTCTCCC+TGG | - | chr1.2:83450291-83450310 | None:intergenic | 40.0% | |
| ATTTCAAATGATGCCTCAGG+TGG | + | chr1.2:83450244-83450263 | MS.gene065015:intron | 40.0% | |
| GAAAGAGCTGTAGATTGAGA+TGG | + | chr1.2:83450056-83450075 | MS.gene065015:intron | 40.0% | |
| GAGCTTCTTATCTTCTTCAG+GGG | - | chr1.2:83449581-83449600 | None:intergenic | 40.0% | |
| GTGGGTATTTGAATTGTGAG+AGG | - | chr1.2:83449790-83449809 | None:intergenic | 40.0% | |
| TCTTCTGACAGCGATGTTAA+AGG | - | chr1.2:83450834-83450853 | None:intergenic | 40.0% | |
| TGACAGCGATGTTAAAGGAT+AGG | - | chr1.2:83450829-83450848 | None:intergenic | 40.0% | |
| TGTGTAGCTAAAGTCCTTTG+CGG | - | chr1.2:83450447-83450466 | None:intergenic | 40.0% | |
| TTTCAAATGATGCCTCAGGT+GGG | + | chr1.2:83450245-83450264 | MS.gene065015:intron | 40.0% | |
| ! | GTAATTTTCACCTTCCCATG+AGG | - | chr1.2:83450712-83450731 | None:intergenic | 40.0% |
| ! | GTCAATCTTCATGAAGCACT+TGG | + | chr1.2:83450142-83450161 | MS.gene065015:intron | 40.0% |
| !! | AAGGCATTGAAGAGAAGAAC+TGG | - | chr1.2:83450629-83450648 | None:intergenic | 40.0% |
| AATGAAGATGCCTCATGGGA+AGG | + | chr1.2:83450699-83450718 | MS.gene065015:CDS | 45.0% | |
| CCAACATCCAACACCAAACA+TGG | - | chr1.2:83449617-83449636 | None:intergenic | 45.0% | |
| TGCGGTGGCATTATCATTGA+TGG | - | chr1.2:83450429-83450448 | None:intergenic | 45.0% | |
| ! | CAATGATAATGCCACCGCAA+AGG | + | chr1.2:83450430-83450449 | MS.gene065015:CDS | 45.0% |
| ! | TTAAGGCCTGGTTTGAAGAG+AGG | + | chr1.2:83450088-83450107 | MS.gene065015:intron | 45.0% |
| !! | CCATGTTTGGTGTTGGATGT+TGG | + | chr1.2:83449614-83449633 | MS.gene065015:CDS | 45.0% |
| ATGCAATTTGAGCCCACCTG+AGG | - | chr1.2:83450260-83450279 | None:intergenic | 50.0% | |
| GTAGCTAAAGTCCTTTGCGG+TGG | - | chr1.2:83450444-83450463 | None:intergenic | 50.0% | |
| TATGAGTTGCAGGGTTGCGA+AGG | + | chr1.2:83450029-83450048 | MS.gene065015:intron | 50.0% | |
| GTTGCAGGGTTGCGAAGGTG+TGG | + | chr1.2:83450034-83450053 | MS.gene065015:intron | 60.0% | |
| TTCTCCCGTGCGTTGCAGCA+CGG | + | chr1.2:83449857-83449876 | MS.gene065015:intron | 60.0% | |
| TTGACCCGTGCTGCAACGCA+CGG | - | chr1.2:83449865-83449884 | None:intergenic | 60.0% | |
| TCTCCCGTGCGTTGCAGCAC+GGG | + | chr1.2:83449858-83449877 | MS.gene065015:intron | 65.0% | |
| TGACCCGTGCTGCAACGCAC+GGG | - | chr1.2:83449864-83449883 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 83449526 | 83450905 | 83449526 | ID=MS.gene065015 |
| chr1.2 | mRNA | 83449526 | 83450905 | 83449526 | ID=MS.gene065015.t1;Parent=MS.gene065015 |
| chr1.2 | exon | 83449526 | 83449658 | 83449526 | ID=MS.gene065015.t1.exon1;Parent=MS.gene065015.t1 |
| chr1.2 | CDS | 83449526 | 83449658 | 83449526 | ID=cds.MS.gene065015.t1;Parent=MS.gene065015.t1 |
| chr1.2 | exon | 83450292 | 83450905 | 83450292 | ID=MS.gene065015.t1.exon2;Parent=MS.gene065015.t1 |
| chr1.2 | CDS | 83450292 | 83450905 | 83450292 | ID=cds.MS.gene065015.t1;Parent=MS.gene065015.t1 |
| Gene Sequence |
| Protein sequence |
