AlfalfaGEDB | MS.gene065197

M. sativa cultivar XinJiangDaYe / MS.gene065197

KEGG: K13425
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065197.t1	KEH20096.1	55.6	401	135	6	1	389	166	535	1.20E-90	343.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065197.t1	Q9SUS1	52.1	96	45	1	151	246	111	205	1.1e-20	102.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065197.t1	A0A072TSA8	55.6	401	135	6	1	389	166	535	8.8e-91	343.6

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene065197.t1	TF	WRKY

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065197.t1	MTR_8g067635	55.039	387	131	6	1	375	166	521	6.17e-123	366
MS.gene065197.t1	MTR_8g067650	53.980	402	131	8	1	389	159	519	1.55e-106	323
MS.gene065197.t1	MTR_2g024380	39.387	424	198	7	1	389	70	469	7.13e-87	271
MS.gene065197.t1	MTR_6g053200	44.164	317	162	5	74	389	233	535	9.57e-76	244
MS.gene065197.t1	MTR_2g024350	48.879	223	111	2	170	389	4	226	5.47e-65	207
MS.gene065197.t1	MTR_8g069935	48.617	253	114	3	1	241	150	398	1.30e-53	184
MS.gene065197.t1	MTR_8g069935	64.444	45	16	0	345	389	418	462	8.15e-11	63.9
MS.gene065197.t1	MTR_7g104980	46.703	182	95	1	195	374	18	199	7.90e-50	167
MS.gene065197.t1	MTR_1g099600	55.000	100	43	2	151	250	163	260	7.89e-29	114
MS.gene065197.t1	MTR_8g028565	50.943	106	49	2	164	267	145	249	6.32e-25	104

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065197.t1	AT4G01250	36.000	200	108	5	48	246	19	199	1.04e-24	103
MS.gene065197.t1	AT5G52830	52.525	99	45	2	151	249	143	239	2.99e-24	102
MS.gene065197.t1	AT4G23550	51.685	89	42	1	158	246	124	211	5.24e-23	98.6
MS.gene065197.t1	AT5G45050	51.282	78	38	0	154	231	1021	1098	6.38e-20	92.4
MS.gene065197.t1	AT5G45050	51.282	78	38	0	154	231	1160	1237	7.39e-20	92.4
MS.gene065197.t1	AT5G45050	51.282	78	38	0	154	231	1132	1209	8.28e-20	92.4
MS.gene065197.t1	AT4G24240	45.763	118	57	3	118	231	224	338	6.22e-19	87.8
MS.gene065197.t1	AT1G29280	61.667	60	23	0	175	234	75	134	8.17e-19	85.9
MS.gene065197.t1	AT4G31550	46.809	94	47	1	149	239	217	310	2.09e-18	85.9
MS.gene065197.t1	AT4G31550	48.352	91	46	1	149	239	222	311	3.07e-18	85.1

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Find 93 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATGACACCTATGTTAG+AGG	0.415313	8.1:-36137465	MS.gene065197:CDS
AAACGACCAAAATTTAAATC+AGG	0.456553	8.1:-36139055	MS.gene065197:CDS
AAACTTGGGTTCTCCTAATG+CGG	0.633652	8.1:-36137765	MS.gene065197:CDS
AAAGATCTAAAACTAGTTTG+TGG	0.559260	8.1:+36139094	None:intergenic
AAAGGTGATGGCAGTGGTGA+TGG	0.430730	8.1:+36139372	None:intergenic
AAATGATAATCAAGTATCAT+TGG	0.431401	8.1:-36138891	MS.gene065197:CDS
AAGGTGATGGCAGTGGTGAT+GGG	0.441027	8.1:+36139373	None:intergenic
AAGTTTGAATCATCTGTTGA+TGG	0.439589	8.1:-36137815	MS.gene065197:CDS
AATCCAATTCTTCAATATGT+TGG	0.427513	8.1:+36137621	None:intergenic
AATGAGAAATCAGAAACGGC+GGG	0.597213	8.1:+36139247	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTTTGAATCATCTGTTGA+TGG	-	chr8.1:36139090-36139109	MS.gene065197:CDS	30.0%
ACTTATGAAGTGACATATCT+AGG	-	chr8.1:36138997-36139016	MS.gene065197:intron	30.0%
AGGTAATGAGAAATCAGAAA+CGG	+	chr8.1:36137665-36137684	None:intergenic	30.0%
ATCTTTCACTTATTGTGGTA+CGG	-	chr8.1:36138771-36138790	MS.gene065197:intron	30.0%
ATGATTAACTCTAGTGCTAA+TGG	-	chr8.1:36137622-36137641	MS.gene065197:CDS	30.0%
ATGGACAAAAACCCATTAAA+GGG	-	chr8.1:36138091-36138110	MS.gene065197:intron	30.0%
ATTAGCACTAGAGTTAATCA+TGG	+	chr8.1:36137623-36137642	None:intergenic	30.0%
GATTGTAGTATTATTCTGGT+TGG	+	chr8.1:36137710-36137729	None:intergenic	30.0%
TAAATGCAACTCTTTGCAAT+GGG	+	chr8.1:36138211-36138230	None:intergenic	30.0%
TAAATTTCGCTTCTGTATAC+GGG	+	chr8.1:36139386-36139405	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	36137415	36139512	36137415	ID=MS.gene065197
chr8.1	mRNA	36137415	36139512	36137415	ID=MS.gene065197.t1;Parent=MS.gene065197
chr8.1	exon	36139049	36139512	36139049	ID=MS.gene065197.t1.exon1;Parent=MS.gene065197.t1
chr8.1	CDS	36139049	36139512	36139049	ID=cds.MS.gene065197.t1;Parent=MS.gene065197.t1
chr8.1	exon	36138800	36138916	36138800	ID=MS.gene065197.t1.exon2;Parent=MS.gene065197.t1
chr8.1	CDS	36138800	36138916	36138800	ID=cds.MS.gene065197.t1;Parent=MS.gene065197.t1
chr8.1	exon	36137415	36138006	36137415	ID=MS.gene065197.t1.exon3;Parent=MS.gene065197.t1
chr8.1	CDS	36137415	36138006	36137415	ID=cds.MS.gene065197.t1;Parent=MS.gene065197.t1

Gene Sequence

>MS.gene065197

ATGCAGCCAAGTGAGTTCACTGAGCTAGAACAATGGATGCTCCAATTCAATCCCCCCACCACTATCCCCATCTCCACCATCACTATTCCCAACTCCACTACCCCTACTACCATTACCCCCATCACCACTGCCATCACCTTTAGTACACCCCCAACAACTATCAACACCCCCACCACCACTAGTCCTATAATCCCTACCACCACAACCATGATTAACTCTAGTGCTAATGGACTCAATCAATATCCCGCCGTTTCTGATTTCTCATTACCTGCCGAACAACAACAGGTTCAAGCCAACCAGAATAATACTACAATCCCCAAATTTCGACGTGAAATCGACCATGAATTAGTTCCACAACAACTCCAGAGAAGTCATAATCAACTACCAATTCAACTACCACAAACTAGTTTTAGATCTTTACCAAATACAAAACCAAAACGACCAAAATTTAAATCAGGAAAAAGGTAAAGTATTGATTCCTATTATTTAATATATTTTACATATAAGTATATTTCATTGTATTAAAATTATTAATTTTTTTTTACATATGTTAACTCCTTGTGACCTAAAATCATTTTACCTTTCTTTTCTTATAGAAAAAATGATAATCAAGTATCATTGGTGAGCTATGTGGGGGCAGATAAGGTTTCAGAGGATTCATGGAGATGGAGAAAATATGGACAAAAACCCATTAAAGGGTCCTCACATCCAAGGTTTTTAAGCATTCAAAACTCTAACGTTCATATATTTTTCTTAATACACACACCCACACCAAGGGTTTTAATTAGTGGTTCCCATTGCAAAGAGTTGCATTTAAATGAGAAAAATATGGTCTTAGTCATAGTTGTGTCTTCTTTTCAATTTTGAAAAAATAAAAAATTGTTAGTCTTATTTTCACTGAACGACACATTTGAAAAATAAAAATTTATTACAAAATAAAATAAAATATTTTAAGATCTTGTATATGCATCACTTTTTGCTTTTAGCCTCTTTGATTTTTTGTTTTGTTTTTGTTGTTCCTTATCTTTTTTCTATAATCCTTGGTATTTCTTTTAGTTCCCACAAATTTATTCAATATTAACATTGGTTTCTTTAATAATAATTTTAACACTTATCTTGATGTACGAAAGTCAAATATTCCATATGAATAAATTTGGCAGGAATTCAAATAAAATAACAGAGACCGAAATAAAAAAGTGGTATATTTGCAAAAGCAAAAAAAAAAATAATACGAAGACTAAATTTAAAAAATATATATTTATAGGAATCAAAAACATATTTAAATCTTTCACCTTATATTTGTAAAATTCATATTTTCCCTTACATAGTGATTTTTATTATGTATAAATTAAACAAATCTTTCACTTATTGTGGTACGGAGTTGGATTGCATAGCTTTTTGTAATATCATAGCCTATATAAATATGTGTTTATATGATTTTAGGTATTAAAATGTTGCAATTTAGCGGTGTTTGATATAAAAAATAATCAATGAATGATTTTGCAGAAGCTACTACAAATGTAGCAGTTCCAGTGATTGTTTAGCAAGAAAACAAGTGGAAAAAATAAAAGATGGAGATGACACTTATGAAGTGACATATCTAGGAGAACACAATCACAATAAACCAGCAAATAATCGGAACTCCATTGTCGATAGCTCTCGGTACATGTCACCAAAGTTTGAATCATCTGTTGATGGAGAAGCTAGATGTTTCACAAACATCATAAACTTGGGTTCTCCTAATGCGGTAATGTTGCAGCTTGATCAGCCAGAGAGCAGCAAGGCTCGGGTTCTTGTCGGTCAATCAAAAATCCCTGATCTAAAAACTGAACTTAGTCCTTTACCTACACTGGGCAAAGCAAAATCTCCCCAACATATTGAAGAATTGGATTCACCTAACATGATGATGTTGCAGTATGATCAGCAGAAGAGCGAAAATTCCAATGTTTTTTCATATGAATCAGAAATCCCGTATACAGAAGCGAAATTTATTGAAAGCTTCAAAGAAGATGAAATTTTGATACCAAACATGACACCTATGTTAGAGGAATTCTTTTTGGATTTCAACAATGTCAATAGTTGTGCTCTATTCCATTAG

Protein sequence

>MS.gene065197.t1

MQPSEFTELEQWMLQFNPPTTIPISTITIPNSTTPTTITPITTAITFSTPPTTINTPTTTSPIIPTTTTMINSSANGLNQYPAVSDFSLPAEQQQVQANQNNTTIPKFRREIDHELVPQQLQRSHNQLPIQLPQTSFRSLPNTKPKRPKFKSGKRKNDNQVSLVSYVGADKVSEDSWRWRKYGQKPIKGSSHPRSYYKCSSSSDCLARKQVEKIKDGDDTYEVTYLGEHNHNKPANNRNSIVDSSRYMSPKFESSVDGEARCFTNIINLGSPNAVMLQLDQPESSKARVLVGQSKIPDLKTELSPLPTLGKAKSPQHIEELDSPNMMMLQYDQQKSENSNVFSYESEIPYTEAKFIESFKEDEILIPNMTPMLEEFFLDFNNVNSCALFH