AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065296

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065296.t1 MTR_1g061660 96.567 233 7 1 1 233 1 232 3.38e-170 468
MS.gene065296.t1 MTR_7g103340 56.735 245 81 10 1 233 1 232 5.30e-79 237
MS.gene065296.t1 MTR_6g007647 55.882 102 38 1 59 153 32 133 7.60e-30 112
MS.gene065296.t1 MTR_6g007647 55.882 102 38 1 59 153 66 167 1.10e-29 112
MS.gene065296.t1 MTR_4g107650 46.575 146 63 4 49 180 84 228 1.84e-29 112
MS.gene065296.t1 MTR_8g468210 55.670 97 36 1 57 146 74 170 7.15e-28 107
MS.gene065296.t1 MTR_6g011610 54.639 97 44 0 54 150 71 167 1.16e-27 106
MS.gene065296.t1 MTR_6g011610 54.639 97 44 0 54 150 90 186 1.19e-27 106
MS.gene065296.t1 MTR_3g086790 38.202 178 88 5 55 221 55 221 7.01e-27 105
MS.gene065296.t1 MTR_5g038280 54.128 109 39 2 58 155 85 193 2.05e-26 104
MS.gene065296.t1 MTR_8g089895 40.645 155 79 4 48 189 39 193 3.61e-26 103
MS.gene065296.t1 MTR_5g039000 53.684 95 37 1 59 146 76 170 4.53e-26 102
MS.gene065296.t1 MTR_3g092150 48.077 104 46 2 62 158 40 142 5.14e-25 99.0
MS.gene065296.t1 MTR_8g469430 53.608 97 38 1 60 149 89 185 5.24e-25 100
MS.gene065296.t1 MTR_3g080100 41.447 152 78 4 59 207 19 162 1.14e-24 97.4
MS.gene065296.t1 MTR_7g010020 55.670 97 36 1 58 147 78 174 1.59e-24 98.6
MS.gene065296.t1 MTR_3g080100 53.763 93 43 0 59 151 58 150 2.23e-24 97.8
MS.gene065296.t1 MTR_8g026960 41.538 130 57 3 64 183 38 158 9.09e-24 95.9
MS.gene065296.t1 MTR_5g019680 43.396 106 60 0 55 160 48 153 2.91e-20 85.5
MS.gene065296.t1 MTR_4g097600 48.315 89 46 0 60 148 127 215 1.65e-18 82.4
MS.gene065296.t1 MTR_4g100550 46.067 89 48 0 60 148 120 208 3.06e-18 81.6
MS.gene065296.t1 MTR_7g093430 44.086 93 52 0 60 152 160 252 3.10e-18 82.0
MS.gene065296.t1 MTR_5g019650 41.593 113 59 1 55 160 48 160 6.99e-18 79.7
MS.gene065296.t1 MTR_1g017090 43.119 109 55 2 47 148 110 218 8.02e-18 80.5
MS.gene065296.t1 MTR_1g054285 44.944 89 49 0 60 148 154 242 1.54e-17 80.1
MS.gene065296.t1 MTR_5g013010 47.191 89 47 0 60 148 136 224 5.54e-17 78.6
MS.gene065296.t1 MTR_2g061030 43.820 89 50 0 60 148 200 288 9.05e-17 78.2
MS.gene065296.t1 MTR_8g006705 44.944 89 49 0 60 148 146 234 1.09e-16 77.8
MS.gene065296.t1 MTR_4g084750 43.617 94 53 0 55 148 60 153 4.47e-16 74.3
MS.gene065296.t1 MTR_5g014890 42.697 89 51 0 60 148 123 211 2.90e-15 73.2
MS.gene065296.t1 MTR_4g126900 43.023 86 49 0 63 148 91 176 3.83e-15 72.0
MS.gene065296.t1 MTR_3g103590 42.697 89 51 0 60 148 145 233 3.17e-14 70.9
MS.gene065296.t1 MTR_2g038625 41.573 89 52 0 60 148 82 170 7.27e-13 65.9
MS.gene065296.t1 MTR_2g038580 41.573 89 52 0 60 148 82 170 7.27e-13 65.9
MS.gene065296.t1 MTR_5g013010 55.769 52 23 0 60 111 136 187 2.96e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065296.t1 AT5G03790 50.256 195 79 6 1 178 1 194 1.57e-48 159
MS.gene065296.t1 AT5G03790 53.704 162 58 5 1 145 1 162 1.98e-42 142
MS.gene065296.t1 AT2G36610 48.603 179 66 7 7 162 5 180 1.20e-40 138
MS.gene065296.t1 AT4G40060 42.405 158 80 3 29 177 30 185 1.27e-31 117
MS.gene065296.t1 AT1G69780 44.000 150 57 3 57 199 82 211 1.25e-29 112
MS.gene065296.t1 AT5G15150 61.290 93 36 0 57 149 112 204 3.17e-29 111
MS.gene065296.t1 AT2G22430 54.455 101 39 1 56 149 58 158 8.70e-29 110
MS.gene065296.t1 AT3G01220 54.128 109 43 1 57 158 84 192 3.47e-28 108
MS.gene065296.t1 AT3G01470 37.647 170 96 3 57 219 65 231 7.99e-28 107
MS.gene065296.t1 AT5G65310 39.869 153 73 5 12 153 28 172 2.64e-27 106
MS.gene065296.t1 AT5G65310 39.869 153 73 5 12 153 10 154 2.85e-27 106
MS.gene065296.t1 AT3G61890 38.650 163 71 3 63 214 33 177 8.70e-27 103
MS.gene065296.t1 AT2G46680 48.624 109 49 1 45 146 17 125 1.07e-26 103
MS.gene065296.t1 AT2G46680 46.957 115 54 1 45 152 17 131 7.45e-26 101
MS.gene065296.t1 AT1G26960 54.082 98 38 1 57 147 68 165 6.94e-25 99.0
MS.gene065296.t1 AT2G18550 39.683 126 72 1 55 176 56 181 2.56e-24 96.7
MS.gene065296.t1 AT4G36740 49.474 95 48 0 55 149 51 145 9.52e-24 95.1
MS.gene065296.t1 AT4G36740 49.474 95 48 0 55 149 50 144 9.72e-24 95.1
MS.gene065296.t1 AT1G27045 49.020 102 45 1 60 154 25 126 1.55e-22 91.3
MS.gene065296.t1 AT1G27045 49.020 102 45 1 60 154 25 126 1.55e-22 91.3
MS.gene065296.t1 AT1G27045 49.020 102 45 1 60 154 68 169 1.63e-22 92.0
MS.gene065296.t1 AT1G27045 49.020 102 45 1 60 154 17 118 1.80e-22 90.9
MS.gene065296.t1 AT5G66700 41.818 110 64 0 60 169 71 180 2.10e-21 89.4
MS.gene065296.t1 AT1G27045 44.144 111 54 2 60 168 68 172 5.95e-21 88.6
MS.gene065296.t1 AT5G53980 45.263 95 52 0 60 154 11 105 1.10e-17 77.4
MS.gene065296.t1 AT4G16780 43.617 94 53 0 55 148 124 217 6.69e-17 78.2
MS.gene065296.t1 AT2G22800 43.617 94 53 0 55 148 108 201 7.92e-17 77.8
MS.gene065296.t1 AT5G06710 44.944 89 49 0 60 148 190 278 1.07e-16 78.2
MS.gene065296.t1 AT4G37790 43.617 94 53 0 55 148 121 214 1.79e-16 76.6
MS.gene065296.t1 AT5G47370 32.653 147 90 2 11 148 72 218 3.39e-16 76.3
MS.gene065296.t1 AT2G01430 47.191 89 47 0 60 148 139 227 4.34e-16 75.9
MS.gene065296.t1 AT4G17460 41.489 94 55 0 55 148 130 223 5.76e-16 75.5
MS.gene065296.t1 AT3G60390 44.944 89 49 0 60 148 162 250 5.77e-16 75.9
MS.gene065296.t1 AT2G44910 42.697 89 51 0 60 148 163 251 4.68e-15 73.2
MS.gene065296.t1 AT1G70920 40.426 94 56 0 55 148 34 127 3.42e-12 63.2
MS.gene065296.t1 AT1G70920 40.426 94 56 0 55 148 64 157 4.06e-12 63.5
MS.gene065296.t1 AT5G06710 53.846 52 24 0 60 111 190 241 1.98e-11 62.8
MS.gene065296.t1 AT3G61150 38.462 104 54 4 57 157 108 204 3.19e-11 63.2
MS.gene065296.t1 AT2G01430 54.717 53 24 0 60 112 139 191 6.08e-11 60.5

Find 48 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCCTCGTCAAATTGCTATT+TGG 0.235729 1.2:+46935688 MS.gene065296:CDS
CCAATTTGAGGTTGTGTCTA+AGG 0.239704 1.2:+46935776 MS.gene065296:CDS
TCCACCTAGCACGTCTGTTT+TGG 0.279067 1.2:-46935713 None:intergenic
AAACTGTTGTTTCAATTTAG+AGG 0.288321 1.2:+46937153 MS.gene065296:CDS
CCTTAGACACAACCTCAAAT+TGG 0.299647 1.2:-46935776 None:intergenic
ATGAAGCTTTCCAAGGAGTT+AGG 0.302805 1.2:+46935660 MS.gene065296:CDS
GAAAGCTTCATCTTCCTTTG+AGG 0.362313 1.2:-46935648 None:intergenic
AATTAAGGGTGATAGTTATA+AGG 0.365223 1.2:-46937229 None:intergenic
AGATACAATGTTCCCAGAAA+TGG 0.367312 1.2:+46935521 MS.gene065296:CDS
CAGCTCTACGTTGCTCCAAC+AGG 0.371599 1.2:+46937086 MS.gene065296:CDS
AAAGCTTCATCTTCCTTTGA+GGG 0.383872 1.2:-46935647 None:intergenic
GCAGGCCAGTAGGGCAATAG+TGG 0.385106 1.2:-46937199 None:intergenic
CTTTCCCTTCACCTCCCTGT+TGG 0.398676 1.2:-46937101 None:intergenic
GTAGTTGTAGAGGAAGCTTA+AGG 0.434188 1.2:-46935089 None:intergenic
GTGTTCCACTATTGCCCTAC+TGG 0.465761 1.2:+46937194 MS.gene065296:CDS
TGAAGCTTTCCAAGGAGTTA+GGG 0.476348 1.2:+46935661 MS.gene065296:CDS
TGGAAGTGAAACAGCAAGAC+TGG 0.490760 1.2:+46935496 MS.gene065296:CDS
TTCACTTAGACACCAATTTG+AGG 0.493024 1.2:+46935764 MS.gene065296:CDS
ATACAAGTGTTCAAGTTGCT+TGG 0.493063 1.2:-46935739 None:intergenic
GGTACAGAAGCAGGCCAGTA+GGG 0.495028 1.2:-46937208 None:intergenic
AGGCTGTAGCCCTAACTCCT+TGG 0.496406 1.2:-46935670 None:intergenic
GTGTGTTTGTTAAGGAATGA+TGG 0.508746 1.2:+46935476 MS.gene065296:intron
AAAACAGAAGCTTCAGGATG+AGG 0.513448 1.2:+46935800 MS.gene065296:CDS
TACAACCAATGCTCATATCA+AGG 0.516637 1.2:+46935109 MS.gene065296:CDS
TAGTTATAAGGTACAGAAGC+AGG 0.517499 1.2:-46937217 None:intergenic
TTGCTCCAACAGGGAGGTGA+AGG 0.517774 1.2:+46937096 MS.gene065296:CDS
CAACAGTTGAACATTGATGA+AGG 0.518077 1.2:+46937130 MS.gene065296:CDS
TGGCCAGGTTATGAAGTTGA+AGG 0.523593 1.2:+46936964 MS.gene065296:intron
AGCATTGGTTGTAGTTGTAG+AGG 0.525273 1.2:-46935099 None:intergenic
TAGATAATTATGGAATGGAA+TGG 0.526946 1.2:+46935031 None:intergenic
AACTCTTGTGGTAGAATGCA+AGG 0.527714 1.2:+46937007 MS.gene065296:CDS
AGGTACAGAAGCAGGCCAGT+AGG 0.540474 1.2:-46937209 None:intergenic
GAGTCAACAGATGGAATCAC+TGG 0.545208 1.2:+46935593 MS.gene065296:CDS
ACAGATAGATAATTATGGAA+TGG 0.549629 1.2:+46935026 None:intergenic
CTTAAGGAAGACTCTGGTTG+AGG 0.556116 1.2:-46935073 None:intergenic
AGATCAAACTAGACCCTCAA+AGG 0.558248 1.2:+46935634 MS.gene065296:CDS
GGTTCCAAAACAGACGTGCT+AGG 0.562576 1.2:+46935709 MS.gene065296:CDS
GCGATTAACGAGTCAACAGA+TGG 0.563350 1.2:+46935584 MS.gene065296:CDS
AACAGTAGAAAGCACATCAG+AGG 0.578567 1.2:+46937063 MS.gene065296:CDS
AAAGAACAAGGTAACTCTTG+TGG 0.599344 1.2:+46936995 MS.gene065296:CDS
AGGAAGCTTAAGGAAGACTC+TGG 0.609695 1.2:-46935079 None:intergenic
AAGGAAGATGAAGCTTTCCA+AGG 0.615641 1.2:+46935653 MS.gene065296:CDS
AGCTCTACGTTGCTCCAACA+GGG 0.620895 1.2:+46937087 MS.gene065296:CDS
AAGGCTATGTTGAAAGAACA+AGG 0.632753 1.2:+46936983 MS.gene065296:CDS
TGCTCCAACAGGGAGGTGAA+GGG 0.637344 1.2:+46937097 MS.gene065296:CDS
TCCAAAACAGACGTGCTAGG+TGG 0.669622 1.2:+46935712 MS.gene065296:CDS
AACCAAATAGCAATTTGACG+AGG 0.698239 1.2:-46935690 None:intergenic
TCTACGTTGCTCCAACAGGG+AGG 0.734444 1.2:+46937090 MS.gene065296:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATCATAAGTTTAAATATA+GGG - chr1.2:46935230-46935249 None:intergenic 10.0%
!! TTAATCATAAGTTTAAATAT+AGG - chr1.2:46935231-46935250 None:intergenic 10.0%
!!! TGTTATTTTAAATAATCAAA+TGG + chr1.2:46936035-46936054 MS.gene065296:intron 10.0%
!!! TTATATATAAAAATTTGTTG+TGG + chr1.2:46936944-46936963 MS.gene065296:intron 10.0%
!!! TTTTATATATAATATGCATA+TGG - chr1.2:46936936-46936955 None:intergenic 10.0%
!!! TTTTCTCTTATTTATATTAT+TGG + chr1.2:46936652-46936671 MS.gene065296:intron 10.0%
!! AAAAAAACTATATATATGCA+TGG - chr1.2:46935276-46935295 None:intergenic 15.0%
!! AAAAAATGTAACTAAAATCA+TGG + chr1.2:46936573-46936592 MS.gene065296:intron 15.0%
!! AGAGAAAAATAAATTATCTA+AGG + chr1.2:46936277-46936296 MS.gene065296:intron 15.0%
!! ATCAAAAACGTATAAATATA+TGG + chr1.2:46936310-46936329 MS.gene065296:intron 15.0%
!!! AAATGAAATCCATTTTTTTT+TGG - chr1.2:46936171-46936190 None:intergenic 15.0%
!!! AGTATTTTAATTTAGACAAT+AGG - chr1.2:46936845-46936864 None:intergenic 15.0%
!!! ATAATTTATTTTTCTCTTCT+TGG - chr1.2:46936274-46936293 None:intergenic 15.0%
!!! TTTTTTTCTTCTTCTATATA+TGG + chr1.2:46935289-46935308 MS.gene065296:intron 15.0%
!! ACAATATTGTTTAGAAGTTT+AGG - chr1.2:46935456-46935475 None:intergenic 20.0%
!! ATTCAAAATTTACGTATGTT+TGG + chr1.2:46935337-46935356 MS.gene065296:intron 20.0%
!! GTAGAAAAAAAATTCTCAAA+TGG + chr1.2:46936381-46936400 MS.gene065296:intron 20.0%
!! TAACTAGAGAAATACTTATA+TGG - chr1.2:46936204-46936223 None:intergenic 20.0%
!! TCATTCTCATATATTATTAC+TGG + chr1.2:46935413-46935432 MS.gene065296:intron 20.0%
!!! AAAAAAATGGATTTCATTTG+TGG + chr1.2:46936172-46936191 MS.gene065296:intron 20.0%
!!! ATTACATTCTAGAAGTTATT+AGG - chr1.2:46935200-46935219 None:intergenic 20.0%
!!! TAAATATATGGTTTTGATGT+CGG + chr1.2:46936322-46936341 MS.gene065296:intron 20.0%
! AAACTATATATATGCATGGT+TGG - chr1.2:46935272-46935291 None:intergenic 25.0%
! ATGGAAAAAATGATGAAGTA+TGG + chr1.2:46935540-46935559 MS.gene065296:CDS 25.0%
! CTTATCTTCTCATTGAAATT+TGG - chr1.2:46936462-46936481 None:intergenic 25.0%
! TAAAATAAACACACCCATAT+TGG - chr1.2:46936743-46936762 None:intergenic 25.0%
! TATATGGCATATATATAGCT+TGG + chr1.2:46935305-46935324 MS.gene065296:intron 25.0%
! TGGAAAAAATGATGAAGTAT+GGG + chr1.2:46935541-46935560 MS.gene065296:CDS 25.0%
!! AATATTGTGTGTGTTTGTTA+AGG + chr1.2:46935468-46935487 MS.gene065296:intron 25.0%
!!! AAACTGTTGTTTCAATTTAG+AGG + chr1.2:46937153-46937172 MS.gene065296:CDS 25.0%
!!! AAATATATGGTTTTGATGTC+GGG + chr1.2:46936323-46936342 MS.gene065296:intron 25.0%
!!! ACCTTTTTTTCATTTGTGTA+TGG - chr1.2:46935991-46936010 None:intergenic 25.0%
!!! CCTTTTTTTCATTTGTGTAT+GGG - chr1.2:46935990-46936009 None:intergenic 25.0%
!!! GAAGTGTATGTTTTGATTTA+CGG + chr1.2:46936506-46936525 MS.gene065296:intron 25.0%
!!! TCATCATTTTTTCCATTTCT+GGG - chr1.2:46935536-46935555 None:intergenic 25.0%
!!! TGAAATCCATTTTTTTTTGG+TGG - chr1.2:46936168-46936187 None:intergenic 25.0%
!!! TTCATCATTTTTTCCATTTC+TGG - chr1.2:46935537-46935556 None:intergenic 25.0%
AGTATTTGTGTGTGTCTAAA+TGG + chr1.2:46936060-46936079 MS.gene065296:intron 30.0%
ATAATCCTCTCAACAAAAAG+AGG + chr1.2:46936426-46936445 MS.gene065296:intron 30.0%
ATACGAATAAGTGTATACGT+AGG + chr1.2:46936246-46936265 MS.gene065296:intron 30.0%
ATGTCGGACGTATAAATATA+TGG + chr1.2:46936338-46936357 MS.gene065296:intron 30.0%
CCCATACACAAATGAAAAAA+AGG + chr1.2:46935987-46936006 MS.gene065296:intron 30.0%
CGTATACACTTATTCGTATA+TGG - chr1.2:46936245-46936264 None:intergenic 30.0%
GAACAATACCTAAATGGTTA+CGG + chr1.2:46935917-46935936 MS.gene065296:intron 30.0%
GACAATGCATAATATATGCA+TGG + chr1.2:46936123-46936142 MS.gene065296:intron 30.0%
GTCAATGAACAATACCTAAA+TGG + chr1.2:46935911-46935930 MS.gene065296:intron 30.0%
TAGACACAGTTTACCAATAT+GGG + chr1.2:46936727-46936746 MS.gene065296:intron 30.0%
! CTAGTCAATTTTACGAGTAT+AGG - chr1.2:46936632-46936651 None:intergenic 30.0%
! TTTTACCTTGATATGAGCAT+TGG - chr1.2:46935117-46935136 None:intergenic 30.0%
!! TATAAAAATTTGTTGTGGCC+AGG + chr1.2:46936949-46936968 MS.gene065296:intron 30.0%
!!! ATCCATTTTTTTTTGGTGGT+CGG - chr1.2:46936164-46936183 None:intergenic 30.0%
AAAGAACAAGGTAACTCTTG+TGG + chr1.2:46936995-46937014 MS.gene065296:CDS 35.0%
AAAGCTTCATCTTCCTTTGA+GGG - chr1.2:46935650-46935669 None:intergenic 35.0%
AACCAAATAGCAATTTGACG+AGG - chr1.2:46935693-46935712 None:intergenic 35.0%
AAGGCTATGTTGAAAGAACA+AGG + chr1.2:46936983-46937002 MS.gene065296:CDS 35.0%
AGATACAATGTTCCCAGAAA+TGG + chr1.2:46935521-46935540 MS.gene065296:CDS 35.0%
ATGCATATGGTTTAACCTGT+AGG - chr1.2:46936923-46936942 None:intergenic 35.0%
CAACAGTTGAACATTGATGA+AGG + chr1.2:46937130-46937149 MS.gene065296:CDS 35.0%
CATAATATATGCATGGTACG+AGG + chr1.2:46936130-46936149 MS.gene065296:intron 35.0%
CGGTGAACTTAATGATATCA+CGG + chr1.2:46936526-46936545 MS.gene065296:intron 35.0%
CTCTCAACAAAAAGAGGTTA+TGG + chr1.2:46936432-46936451 MS.gene065296:intron 35.0%
GAAACAAAGTTGCAGTGTTA+TGG - chr1.2:46936771-46936790 None:intergenic 35.0%
GTAGACACAGTTTACCAATA+TGG + chr1.2:46936726-46936745 MS.gene065296:intron 35.0%
TAAGGAAAAACAGAAGCTTC+AGG + chr1.2:46935794-46935813 MS.gene065296:CDS 35.0%
TACAACCAATGCTCATATCA+AGG + chr1.2:46935109-46935128 MS.gene065296:CDS 35.0%
TAGTTATAAGGTACAGAAGC+AGG - chr1.2:46937220-46937239 None:intergenic 35.0%
TTCACTTAGACACCAATTTG+AGG + chr1.2:46935764-46935783 MS.gene065296:CDS 35.0%
! CATAACCTCTTTTTGTTGAG+AGG - chr1.2:46936434-46936453 None:intergenic 35.0%
! GTGTGTTTGTTAAGGAATGA+TGG + chr1.2:46935476-46935495 MS.gene065296:intron 35.0%
!! ATACAAGTGTTCAAGTTGCT+TGG - chr1.2:46935742-46935761 None:intergenic 35.0%
!!! TCCATTTTTTTTTGGTGGTC+GGG - chr1.2:46936163-46936182 None:intergenic 35.0%
AAAACAGAAGCTTCAGGATG+AGG + chr1.2:46935800-46935819 MS.gene065296:CDS 40.0%
AACTCTTGTGGTAGAATGCA+AGG + chr1.2:46937007-46937026 MS.gene065296:CDS 40.0%
AAGGAAGATGAAGCTTTCCA+AGG + chr1.2:46935653-46935672 MS.gene065296:CDS 40.0%
ACACGCGTAACTACATACAA+TGG + chr1.2:46935387-46935406 MS.gene065296:intron 40.0%
ACTGTGTCTACAAAAGAGAC+AGG - chr1.2:46936717-46936736 None:intergenic 40.0%
AGATCAAACTAGACCCTCAA+AGG + chr1.2:46935634-46935653 MS.gene065296:CDS 40.0%
ATGAAGCTTTCCAAGGAGTT+AGG + chr1.2:46935660-46935679 MS.gene065296:CDS 40.0%
CCTTAGACACAACCTCAAAT+TGG - chr1.2:46935779-46935798 None:intergenic 40.0%
CTGTGTCTACAAAAGAGACA+GGG - chr1.2:46936716-46936735 None:intergenic 40.0%
GAAAGCTTCATCTTCCTTTG+AGG - chr1.2:46935651-46935670 None:intergenic 40.0%
GAGTATGTGACATATCCTAC+AGG + chr1.2:46936905-46936924 MS.gene065296:intron 40.0%
GTAGTTGTAGAGGAAGCTTA+AGG - chr1.2:46935092-46935111 None:intergenic 40.0%
TAACACAGCCGTAACCATTT+AGG - chr1.2:46935928-46935947 None:intergenic 40.0%
TAGCCTTCAACTTCATAACC+TGG - chr1.2:46936970-46936989 None:intergenic 40.0%
TGAAGCTTTCCAAGGAGTTA+GGG + chr1.2:46935661-46935680 MS.gene065296:CDS 40.0%
TGTGTCTACAAAAGAGACAG+GGG - chr1.2:46936715-46936734 None:intergenic 40.0%
! AACAGTAGAAAGCACATCAG+AGG + chr1.2:46937063-46937082 MS.gene065296:CDS 40.0%
! AGCCTCGTCAAATTGCTATT+TGG + chr1.2:46935688-46935707 MS.gene065296:CDS 40.0%
! CCAATTTGAGGTTGTGTCTA+AGG + chr1.2:46935776-46935795 MS.gene065296:CDS 40.0%
!! ACGGCTGTGTTATTTAGATG+AGG + chr1.2:46935936-46935955 MS.gene065296:intron 40.0%
!! ACTGGAGAGTAGTTTTCAAG+AGG + chr1.2:46935611-46935630 MS.gene065296:CDS 40.0%
!! AGCATTGGTTGTAGTTGTAG+AGG - chr1.2:46935102-46935121 None:intergenic 40.0%
!! CGGCTGTGTTATTTAGATGA+GGG + chr1.2:46935937-46935956 MS.gene065296:intron 40.0%
!!! CCATTTTTTTTTGGTGGTCG+GGG - chr1.2:46936162-46936181 None:intergenic 40.0%
AGGAAGCTTAAGGAAGACTC+TGG - chr1.2:46935082-46935101 None:intergenic 45.0%
CCCCGACCACCAAAAAAAAA+TGG + chr1.2:46936159-46936178 MS.gene065296:intron 45.0%
CTTAAGGAAGACTCTGGTTG+AGG - chr1.2:46935076-46935095 None:intergenic 45.0%
GAGTCAACAGATGGAATCAC+TGG + chr1.2:46935593-46935612 MS.gene065296:CDS 45.0%
GCGATTAACGAGTCAACAGA+TGG + chr1.2:46935584-46935603 MS.gene065296:CDS 45.0%
GTCGATATCATGTTGGACAC+TGG - chr1.2:46935369-46935388 None:intergenic 45.0%
TGGAAGTGAAACAGCAAGAC+TGG + chr1.2:46935496-46935515 MS.gene065296:CDS 45.0%
TGGCCAGGTTATGAAGTTGA+AGG + chr1.2:46936964-46936983 MS.gene065296:intron 45.0%
! TACGCGTGTCGATATCATGT+TGG - chr1.2:46935376-46935395 None:intergenic 45.0%
! TGAGCTTAGCTCAGTTGGTA+TGG + chr1.2:46936101-46936120 MS.gene065296:intron 45.0%
ACAAAAGAGACAGGGGAGAG+AGG - chr1.2:46936708-46936727 None:intergenic 50.0%
AGCTCTACGTTGCTCCAACA+GGG + chr1.2:46937087-46937106 MS.gene065296:CDS 50.0%
GGTTCCAAAACAGACGTGCT+AGG + chr1.2:46935709-46935728 MS.gene065296:CDS 50.0%
GTGTTCCACTATTGCCCTAC+TGG + chr1.2:46937194-46937213 MS.gene065296:CDS 50.0%
TCCAAAACAGACGTGCTAGG+TGG + chr1.2:46935712-46935731 MS.gene065296:CDS 50.0%
! TCCACCTAGCACGTCTGTTT+TGG - chr1.2:46935716-46935735 None:intergenic 50.0%
AGGCTGTAGCCCTAACTCCT+TGG - chr1.2:46935673-46935692 None:intergenic 55.0%
AGGTACAGAAGCAGGCCAGT+AGG - chr1.2:46937212-46937231 None:intergenic 55.0%
CAGCTCTACGTTGCTCCAAC+AGG + chr1.2:46937086-46937105 MS.gene065296:CDS 55.0%
CCAACTGAGCTAAGCTCACG+AGG - chr1.2:46936099-46936118 None:intergenic 55.0%
CCTCGTGAGCTTAGCTCAGT+TGG + chr1.2:46936096-46936115 MS.gene065296:intron 55.0%
CTTTCCCTTCACCTCCCTGT+TGG - chr1.2:46937104-46937123 None:intergenic 55.0%
GGTACAGAAGCAGGCCAGTA+GGG - chr1.2:46937211-46937230 None:intergenic 55.0%
TCTACGTTGCTCCAACAGGG+AGG + chr1.2:46937090-46937109 MS.gene065296:CDS 55.0%
TGCTCCAACAGGGAGGTGAA+GGG + chr1.2:46937097-46937116 MS.gene065296:CDS 55.0%
TTGCTCCAACAGGGAGGTGA+AGG + chr1.2:46937096-46937115 MS.gene065296:CDS 55.0%
GCAGGCCAGTAGGGCAATAG+TGG - chr1.2:46937202-46937221 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 46935040 46937249 46935040 ID=MS.gene065296
chr1.2 mRNA 46935040 46937249 46935040 ID=MS.gene065296.t1;Parent=MS.gene065296
chr1.2 exon 46935040 46935130 46935040 ID=MS.gene065296.t1.exon1;Parent=MS.gene065296.t1
chr1.2 CDS 46935040 46935130 46935040 ID=cds.MS.gene065296.t1;Parent=MS.gene065296.t1
chr1.2 exon 46935490 46935821 46935490 ID=MS.gene065296.t1.exon2;Parent=MS.gene065296.t1
chr1.2 CDS 46935490 46935821 46935490 ID=cds.MS.gene065296.t1;Parent=MS.gene065296.t1
chr1.2 exon 46936971 46937249 46936971 ID=MS.gene065296.t1.exon3;Parent=MS.gene065296.t1
chr1.2 CDS 46936971 46937249 46936971 ID=cds.MS.gene065296.t1;Parent=MS.gene065296.t1
Gene Sequence

>MS.gene065296

ATGGAATGGAATGGAAACACAAGAAGCTTTGTTCCTCAACCAGAGTCTTCCTTAAGCTTCCTCTACAACTACAACCAATGCTCATATCAAGGTAAAAATTATCATCACAACACTAGCTACTCTATTGCATTCATCTTTTTATCATCATAACATTGCACCTAATAACTTCTAGAATGTAATTTAGTAACCCTATATTTAAACTTATGATTAAATTTAATATATTTTCTGTCCAACCATGCATATATATAGTTTTTTTCTTCTTCTATATATGGCATATATATAGCTTGGTCAACATTCATTCAAAATTTACGTATGTTTGGCGCGTGCCAGTGTCCAACATGATATCGACACGCGTAACTACATACAATGGCTTTCATTCTCATATATTATTACTGGTGTCTACATATCAGTGTCCTAAACTTCTAAACAATATTGTGTGTGTTTGTTAAGGAATGATGGAAGTGAAACAGCAAGACTGGACAGATACAATGTTCCCAGAAATGGAAAAAATGATGAAGTATGGGAACTTGCAAGAAAAGAAAAAGCGATTAACGAGTCAACAGATGGAATCACTGGAGAGTAGTTTTCAAGAGGAGATCAAACTAGACCCTCAAAGGAAGATGAAGCTTTCCAAGGAGTTAGGGCTACAGCCTCGTCAAATTGCTATTTGGTTCCAAAACAGACGTGCTAGGTGGAAGACCAAGCAACTTGAACACTTGTATGATTCACTTAGACACCAATTTGAGGTTGTGTCTAAGGAAAAACAGAAGCTTCAGGATGAGGTACGTTAACATATGTAAATATGTATACTATATACTATGCATGTATAGTGAATGTTTTATATACTGAAAACAGAAAACATGATAAATATGTCAATGAACAATACCTAAATGGTTACGGCTGTGTTATTTAGATGAGGGACACAAGCGCAAATAATTTTATTTTAGCCCATACACAAATGAAAAAAAGGTAAAACAAAAAACACTTAAATAATATGTTATTTTAAATAATCAAATGGTAAGTATTTGTGTGTGTCTAAATGGATTTTATTTGTGTCCTCGTGAGCTTAGCTCAGTTGGTATGGACAATGCATAATATATGCATGGTACGAGGTTCGAACCCCGACCACCAAAAAAAAATGGATTTCATTTGTGGTTGTATCCATATAAGTATTTCTCTAGTTAAACACACTTTAAACAATACCATATACGAATAAGTGTATACGTAGGATCCAAGAAGAGAAAAATAAATTATCTAAGGTGAGAAAAAAATCAAAAACGTATAAATATATGGTTTTGATGTCGGACGTATAAATATATGGTTTTGATGTCGGGTTTTTATGTAGAAAAAAAATTCTCAAATGGTCAATTTTATTTTTTTTTTGTCATAATCCTCTCAACAAAAAGAGGTTATGGTAGTCCAAATTTCAATGAGAAGATAAGTAAAGTTTGACTAATTTTAACAATGAAGTGTATGTTTTGATTTACGGTGAACTTAATGATATCACGGTTACATTGTAATTTTGTTGAAATTAAAAAATGTAACTAAAATCATGGTTGCACACTGTGAATTGTGATTCTGCAAACTCACCTATACTCGTAAAATTGACTAGTTTTCTCTTATTTATATTATTGGTGTTAAGTAAACTTTCAAAGTGATTGTATGCCTCTCTCCCCTGTCTCTTTTGTAGACACAGTTTACCAATATGGGTGTGTTTATTTTAAAAATCCATAACACTGCAACTTTGTTTCATGATTTTACAAGTAAATCGAAGATACTTTGCTACTTCGATATTTAATACTCCTATTGTCTAAATTAAAATACTGTTTAATTGTTTTTGCTGCTAAAGTTTGAAATAGACACGTGAGTATGTGACATATCCTACAGGTTAAACCATATGCATATTATATATAAAAATTTGTTGTGGCCAGGTTATGAAGTTGAAGGCTATGTTGAAAGAACAAGGTAACTCTTGTGGTAGAATGCAAGGTTACTATACAGAAATGTCAGTGAATGAAACAGAAACAGTAGAAAGCACATCAGAGGCAGCTCTACGTTGCTCCAACAGGGAGGTGAAGGGAAAGATTCATCAACAGTTGAACATTGATGAAGGAAACTGTTGTTTCAATTTAGAGGACTATAACATTAATAACAGTGTTCCACTATTGCCCTACTGGCCTGCTTCTGTACCTTATAACTATCACCCTTAA
Protein sequence

>MS.gene065296.t1

MEWNGNTRSFVPQPESSLSFLYNYNQCSYQGMMEVKQQDWTDTMFPEMEKMMKYGNLQEKKKRLTSQQMESLESSFQEEIKLDPQRKMKLSKELGLQPRQIAIWFQNRRARWKTKQLEHLYDSLRHQFEVVSKEKQKLQDEVMKLKAMLKEQGNSCGRMQGYYTEMSVNETETVESTSEAALRCSNREVKGKIHQQLNIDEGNCCFNLEDYNINNSVPLLPYWPASVPYNYHP