Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065363.t1 | XP_027922914.1 | 50.7 | 146 | 72 | 0 | 42 | 187 | 39 | 184 | 3.60E-35 | 158.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065363.t1 | A0A2K3JN18 | 53.6 | 138 | 63 | 1 | 48 | 184 | 32 | 169 | 7.5e-35 | 156.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene065363.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065363.t1 | MTR_0856s0010 | 43.023 | 172 | 84 | 4 | 3 | 173 | 2 | 160 | 1.56e-37 | 127 |
| MS.gene065363.t1 | MTR_1g019850 | 29.697 | 165 | 99 | 6 | 17 | 175 | 43 | 196 | 3.07e-15 | 71.6 |
| MS.gene065363.t1 | MTR_7g115320 | 54.545 | 55 | 21 | 2 | 132 | 185 | 3 | 54 | 8.77e-12 | 58.9 |
| MS.gene065363.t1 | MTR_7g446060 | 45.902 | 61 | 33 | 0 | 130 | 190 | 7 | 67 | 1.96e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTGTTGACGTTTCGGATTC+CGG | 0.204382 | 7.4:-10478506 | MS.gene065363:CDS |
| GATTGCAAACCCTTGTTTCA+TGG | 0.302647 | 7.4:+10478379 | None:intergenic |
| GTGTTGACGTTTCGGATTCC+GGG | 0.344560 | 7.4:-10478505 | MS.gene065363:CDS |
| TTGCTTTAAAGAAGGGTCTA+TGG | 0.361166 | 7.4:-10478208 | MS.gene065363:CDS |
| TGCGGCTCGTGTTGACGTTT+CGG | 0.362249 | 7.4:-10478513 | MS.gene065363:CDS |
| ACTAAATCACGGTTGTTTGC+TGG | 0.375082 | 7.4:-10478140 | MS.gene065363:CDS |
| AAAGGATGATCGATCAATAT+AGG | 0.415391 | 7.4:+10478566 | None:intergenic |
| TTGGTGGTATGTTTGATTCA+TGG | 0.433399 | 7.4:+10478282 | None:intergenic |
| GCAAACAACCGTGATTTAGT+TGG | 0.439208 | 7.4:+10478143 | None:intergenic |
| CCATCAGCTAGCTCTCCGTC+AGG | 0.451558 | 7.4:+10478536 | None:intergenic |
| AAAACTAGAAACTCTAAGAA+AGG | 0.460138 | 7.4:-10478356 | MS.gene065363:CDS |
| AAACTAGAAACTCTAAGAAA+GGG | 0.460938 | 7.4:-10478355 | MS.gene065363:CDS |
| GATAACGGTTGCTTTAAAGA+AGG | 0.473052 | 7.4:-10478216 | MS.gene065363:CDS |
| GAGCAATCAAGTTTCTTCGT+TGG | 0.480101 | 7.4:+10478245 | None:intergenic |
| CACTTATGCTCGATGGCTGA+TGG | 0.482201 | 7.4:-10478593 | None:intergenic |
| ATTTGTTGTTTCAAAGGTCC+CGG | 0.485747 | 7.4:+10478487 | None:intergenic |
| GACAGCATTTGTTGTTTCAA+AGG | 0.491833 | 7.4:+10478481 | None:intergenic |
| CTTGTTTCATGGCATATTCA+CGG | 0.492232 | 7.4:+10478390 | None:intergenic |
| CGTGAATATGCCATGAAACA+AGG | 0.498225 | 7.4:-10478389 | MS.gene065363:CDS |
| TTAAAAGACATACCGATAGT+AGG | 0.501742 | 7.4:+10478440 | None:intergenic |
| GTTGGATTCGTCTTCAAAGT+TGG | 0.502236 | 7.4:+10478263 | None:intergenic |
| AAACGATCAGGCAGGTGTAC+GGG | 0.512319 | 7.4:-10478075 | MS.gene065363:CDS |
| ATAACGGTTGCTTTAAAGAA+GGG | 0.522275 | 7.4:-10478215 | MS.gene065363:CDS |
| GAACAAAAGTTTCCGTTCCA+TGG | 0.529264 | 7.4:-10478051 | MS.gene065363:intron |
| GGATTCGTCTTCAAAGTTGG+TGG | 0.530111 | 7.4:+10478266 | None:intergenic |
| TAAACGATCAGGCAGGTGTA+CGG | 0.533293 | 7.4:-10478076 | MS.gene065363:CDS |
| CATCAGCTAGCTCTCCGTCA+GGG | 0.541329 | 7.4:+10478537 | None:intergenic |
| TGATTGCTCAGCTAAGATAA+CGG | 0.550952 | 7.4:-10478231 | MS.gene065363:CDS |
| GATAAATTCACGCCTACTAT+CGG | 0.551302 | 7.4:-10478452 | MS.gene065363:CDS |
| ATTCTAACTTGTGCAAGAGA+AGG | 0.552020 | 7.4:-10478308 | MS.gene065363:CDS |
| AACTCTAAGAAAGGGGCCGA+TGG | 0.560180 | 7.4:-10478347 | MS.gene065363:CDS |
| ATCAGCTAGCTCTCCGTCAG+GGG | 0.582853 | 7.4:+10478538 | None:intergenic |
| GTGAATATGCCATGAAACAA+GGG | 0.602322 | 7.4:-10478388 | MS.gene065363:CDS |
| CCTGACGGAGAGCTAGCTGA+TGG | 0.623440 | 7.4:-10478536 | MS.gene065363:CDS |
| CGGCATCACATACCATGGAA+CGG | 0.625469 | 7.4:+10478039 | None:intergenic |
| AACTAGAAACTCTAAGAAAG+GGG | 0.633083 | 7.4:-10478354 | MS.gene065363:CDS |
| GGATGATCGATCAATATAGG+AGG | 0.639611 | 7.4:+10478569 | None:intergenic |
| AGATAAGTCCAACTAAATCA+CGG | 0.645312 | 7.4:-10478151 | MS.gene065363:CDS |
| TCAGCTAGCTCTCCGTCAGG+GGG | 0.662037 | 7.4:+10478539 | None:intergenic |
| CGGAGAGCTAGCTGATGGTG+CGG | 0.662675 | 7.4:-10478531 | MS.gene065363:CDS |
| AGATTGATATGCATGCACAG+CGG | 0.798983 | 7.4:-10478109 | MS.gene065363:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATAGATGGATAGTTAAAA+TGG | + | chr7.4:10478507-10478526 | None:intergenic | 20.0% |
| !! | TTAAAATGGTACAATACATA+TGG | + | chr7.4:10478493-10478512 | None:intergenic | 20.0% |
| ! | AAACGATATTATTTACACGT+CGG | + | chr7.4:10478554-10478573 | None:intergenic | 25.0% |
| !! | AAAACTAGAAACTCTAAGAA+AGG | - | chr7.4:10478104-10478123 | MS.gene065363:CDS | 25.0% |
| !! | AAACTAGAAACTCTAAGAAA+GGG | - | chr7.4:10478105-10478124 | MS.gene065363:CDS | 25.0% |
| !! | TATCGGTATGTCTTTTAAAT+CGG | - | chr7.4:10478025-10478044 | MS.gene065363:intron | 25.0% |
| !!! | GAAATATTTTAACATGCCAT+CGG | + | chr7.4:10478132-10478151 | None:intergenic | 25.0% |
| AAAGGATGATCGATCAATAT+AGG | + | chr7.4:10477897-10477916 | None:intergenic | 30.0% | |
| AGATAAGTCCAACTAAATCA+CGG | - | chr7.4:10478309-10478328 | MS.gene065363:CDS | 30.0% | |
| AGGTACAACAGTTATATAGA+TGG | + | chr7.4:10478520-10478539 | None:intergenic | 30.0% | |
| TCTATATAACTGTTGTACCT+TGG | - | chr7.4:10478520-10478539 | MS.gene065363:CDS | 30.0% | |
| TTAAAAGACATACCGATAGT+AGG | + | chr7.4:10478023-10478042 | None:intergenic | 30.0% | |
| ! | AACTAGAAACTCTAAGAAAG+GGG | - | chr7.4:10478106-10478125 | MS.gene065363:CDS | 30.0% |
| !! | ATAACGGTTGCTTTAAAGAA+GGG | - | chr7.4:10478245-10478264 | MS.gene065363:CDS | 30.0% |
| !! | GACAATTTTGATAAACGATC+AGG | - | chr7.4:10478373-10478392 | MS.gene065363:CDS | 30.0% |
| CAGATAGTCCTAGTAATACA+TGG | + | chr7.4:10478463-10478482 | None:intergenic | 35.0% | |
| CTTGTTTCATGGCATATTCA+CGG | + | chr7.4:10478073-10478092 | None:intergenic | 35.0% | |
| GACTATCTCCATGTATTACT+AGG | - | chr7.4:10478452-10478471 | MS.gene065363:CDS | 35.0% | |
| GATAAATTCACGCCTACTAT+CGG | - | chr7.4:10478008-10478027 | MS.gene065363:intron | 35.0% | |
| GTGAATATGCCATGAAACAA+GGG | - | chr7.4:10478072-10478091 | MS.gene065363:CDS | 35.0% | |
| TGATTGCTCAGCTAAGATAA+CGG | - | chr7.4:10478229-10478248 | MS.gene065363:CDS | 35.0% | |
| ! | ATTCTAACTTGTGCAAGAGA+AGG | - | chr7.4:10478152-10478171 | MS.gene065363:CDS | 35.0% |
| ! | TTGGTGGTATGTTTGATTCA+TGG | + | chr7.4:10478181-10478200 | None:intergenic | 35.0% |
| !! | ATTTGTTGTTTCAAAGGTCC+CGG | + | chr7.4:10477976-10477995 | None:intergenic | 35.0% |
| !! | ATTTTGATAAACGATCAGGC+AGG | - | chr7.4:10478377-10478396 | MS.gene065363:CDS | 35.0% |
| !! | GACAGCATTTGTTGTTTCAA+AGG | + | chr7.4:10477982-10478001 | None:intergenic | 35.0% |
| !! | GATAACGGTTGCTTTAAAGA+AGG | - | chr7.4:10478244-10478263 | MS.gene065363:CDS | 35.0% |
| !! | TTGCTTTAAAGAAGGGTCTA+TGG | - | chr7.4:10478252-10478271 | MS.gene065363:CDS | 35.0% |
| ACTAAATCACGGTTGTTTGC+TGG | - | chr7.4:10478320-10478339 | MS.gene065363:CDS | 40.0% | |
| AGATTGATATGCATGCACAG+CGG | - | chr7.4:10478351-10478370 | MS.gene065363:CDS | 40.0% | |
| ATGGAGATAGTCATAACCAC+CGG | + | chr7.4:10478444-10478463 | None:intergenic | 40.0% | |
| CGTGAATATGCCATGAAACA+AGG | - | chr7.4:10478071-10478090 | MS.gene065363:CDS | 40.0% | |
| GAACAAAAGTTTCCGTTCCA+TGG | - | chr7.4:10478409-10478428 | MS.gene065363:CDS | 40.0% | |
| GAGCAATCAAGTTTCTTCGT+TGG | + | chr7.4:10478218-10478237 | None:intergenic | 40.0% | |
| GCAAACAACCGTGATTTAGT+TGG | + | chr7.4:10478320-10478339 | None:intergenic | 40.0% | |
| GGATGATCGATCAATATAGG+AGG | + | chr7.4:10477894-10477913 | None:intergenic | 40.0% | |
| GTTGGATTCGTCTTCAAAGT+TGG | + | chr7.4:10478200-10478219 | None:intergenic | 40.0% | |
| ! | GATTGCAAACCCTTGTTTCA+TGG | + | chr7.4:10478084-10478103 | None:intergenic | 40.0% |
| ACACGTCGGATAGAAAACCA+AGG | + | chr7.4:10478540-10478559 | None:intergenic | 45.0% | |
| CATCCTTTATTACCCCCTGA+CGG | - | chr7.4:10477909-10477928 | MS.gene065363:intron | 45.0% | |
| GGATTCGTCTTCAAAGTTGG+TGG | + | chr7.4:10478197-10478216 | None:intergenic | 45.0% | |
| TAAACGATCAGGCAGGTGTA+CGG | - | chr7.4:10478384-10478403 | MS.gene065363:CDS | 45.0% | |
| AAACGATCAGGCAGGTGTAC+GGG | - | chr7.4:10478385-10478404 | MS.gene065363:CDS | 50.0% | |
| CGGCATCACATACCATGGAA+CGG | + | chr7.4:10478424-10478443 | None:intergenic | 50.0% | |
| CGTTCCATGGTATGTGATGC+CGG | - | chr7.4:10478422-10478441 | MS.gene065363:CDS | 50.0% | |
| TCTCCGTCAGGGGGTAATAA+AGG | + | chr7.4:10477915-10477934 | None:intergenic | 50.0% | |
| ! | AACTCTAAGAAAGGGGCCGA+TGG | - | chr7.4:10478113-10478132 | MS.gene065363:CDS | 50.0% |
| ! | CGTGTTGACGTTTCGGATTC+CGG | - | chr7.4:10477954-10477973 | MS.gene065363:intron | 50.0% |
| ! | GTGTTGACGTTTCGGATTCC+GGG | - | chr7.4:10477955-10477974 | MS.gene065363:intron | 50.0% |
| ACCACCGGCATCACATACCA+TGG | + | chr7.4:10478429-10478448 | None:intergenic | 55.0% | |
| TCCATGGTATGTGATGCCGG+TGG | - | chr7.4:10478425-10478444 | MS.gene065363:CDS | 55.0% | |
| ! | ATCAGCTAGCTCTCCGTCAG+GGG | + | chr7.4:10477925-10477944 | None:intergenic | 55.0% |
| ! | CATCAGCTAGCTCTCCGTCA+GGG | + | chr7.4:10477926-10477945 | None:intergenic | 55.0% |
| ! | TGCGGCTCGTGTTGACGTTT+CGG | - | chr7.4:10477947-10477966 | MS.gene065363:intron | 55.0% |
| CCTGACGGAGAGCTAGCTGA+TGG | - | chr7.4:10477924-10477943 | MS.gene065363:intron | 60.0% | |
| ! | CCATCAGCTAGCTCTCCGTC+AGG | + | chr7.4:10477927-10477946 | None:intergenic | 60.0% |
| ! | CGGAGAGCTAGCTGATGGTG+CGG | - | chr7.4:10477929-10477948 | MS.gene065363:intron | 60.0% |
| ! | TCAGCTAGCTCTCCGTCAGG+GGG | + | chr7.4:10477924-10477943 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 10477879 | 10478603 | 10477879 | ID=MS.gene065363 |
| chr7.4 | mRNA | 10477879 | 10478603 | 10477879 | ID=MS.gene065363.t1;Parent=MS.gene065363 |
| chr7.4 | exon | 10478052 | 10478603 | 10478052 | ID=MS.gene065363.t1.exon1;Parent=MS.gene065363.t1 |
| chr7.4 | CDS | 10478052 | 10478603 | 10478052 | ID=cds.MS.gene065363.t1;Parent=MS.gene065363.t1 |
| chr7.4 | exon | 10477879 | 10477905 | 10477879 | ID=MS.gene065363.t1.exon2;Parent=MS.gene065363.t1 |
| chr7.4 | CDS | 10477879 | 10477905 | 10477879 | ID=cds.MS.gene065363.t1;Parent=MS.gene065363.t1 |
| Gene Sequence |
| Protein sequence |
