Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065423.t1 | XP_003624327.1 | 94.7 | 114 | 6 | 0 | 1 | 114 | 947 | 1060 | 2.70E-54 | 221.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065423.t1 | Q8VZG8 | 52.9 | 87 | 36 | 2 | 1 | 86 | 955 | 1037 | 1.6e-15 | 83.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065423.t1 | G7KU95 | 94.7 | 114 | 6 | 0 | 1 | 114 | 947 | 1060 | 1.9e-54 | 221.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065423.t1 | MTR_7g081780 | 94.737 | 114 | 6 | 0 | 1 | 114 | 947 | 1060 | 3.67e-70 | 226 |
| MS.gene065423.t1 | MTR_7g081480 | 87.500 | 112 | 14 | 0 | 1 | 112 | 949 | 1060 | 1.27e-62 | 205 |
| MS.gene065423.t1 | MTR_7g481630 | 76.991 | 113 | 25 | 1 | 1 | 112 | 80 | 192 | 1.87e-57 | 176 |
| MS.gene065423.t1 | MTR_7g481590 | 76.991 | 113 | 25 | 1 | 1 | 112 | 203 | 315 | 2.57e-55 | 174 |
| MS.gene065423.t1 | MTR_7g081570 | 76.106 | 113 | 26 | 1 | 1 | 112 | 939 | 1051 | 1.08e-52 | 177 |
| MS.gene065423.t1 | MTR_7g081410 | 74.336 | 113 | 28 | 1 | 1 | 112 | 826 | 938 | 1.48e-51 | 174 |
| MS.gene065423.t1 | MTR_7g081410 | 74.336 | 113 | 28 | 1 | 1 | 112 | 946 | 1058 | 1.82e-51 | 174 |
| MS.gene065423.t1 | MTR_7g081720 | 53.509 | 114 | 51 | 1 | 1 | 112 | 964 | 1077 | 5.99e-32 | 118 |
| MS.gene065423.t1 | MTR_5g085700 | 54.464 | 112 | 51 | 0 | 2 | 113 | 921 | 1032 | 2.94e-31 | 116 |
| MS.gene065423.t1 | MTR_8g445960 | 49.000 | 100 | 50 | 1 | 1 | 99 | 63 | 162 | 2.95e-28 | 101 |
| MS.gene065423.t1 | MTR_8g044940 | 49.000 | 100 | 50 | 1 | 1 | 99 | 63 | 162 | 2.95e-28 | 101 |
| MS.gene065423.t1 | MTR_1g039080 | 52.525 | 99 | 46 | 1 | 1 | 98 | 192 | 290 | 1.46e-27 | 102 |
| MS.gene065423.t1 | MTR_1g039310 | 48.246 | 114 | 55 | 2 | 3 | 112 | 1051 | 1164 | 5.57e-27 | 103 |
| MS.gene065423.t1 | MTR_1g038890 | 52.525 | 99 | 46 | 1 | 1 | 98 | 1033 | 1131 | 8.52e-27 | 103 |
| MS.gene065423.t1 | MTR_7g045510 | 53.261 | 92 | 41 | 1 | 2 | 93 | 1091 | 1180 | 1.00e-24 | 97.8 |
| MS.gene065423.t1 | MTR_7g039330 | 53.261 | 92 | 41 | 1 | 2 | 93 | 1189 | 1278 | 1.70e-24 | 97.1 |
| MS.gene065423.t1 | MTR_0400s0040 | 48.454 | 97 | 46 | 1 | 1 | 93 | 1058 | 1154 | 3.25e-24 | 96.3 |
| MS.gene065423.t1 | MTR_0365s0030 | 48.454 | 97 | 46 | 1 | 1 | 93 | 1058 | 1154 | 3.25e-24 | 96.3 |
| MS.gene065423.t1 | MTR_7g007540 | 42.982 | 114 | 60 | 2 | 1 | 113 | 1 | 110 | 6.55e-23 | 86.3 |
| MS.gene065423.t1 | MTR_7g007560 | 44.737 | 114 | 58 | 2 | 1 | 113 | 653 | 762 | 1.40e-22 | 91.7 |
| MS.gene065423.t1 | MTR_7g045710 | 50.000 | 94 | 42 | 1 | 2 | 95 | 972 | 1060 | 1.82e-22 | 91.3 |
| MS.gene065423.t1 | MTR_7g051040 | 43.478 | 115 | 56 | 2 | 1 | 108 | 843 | 955 | 5.01e-22 | 89.7 |
| MS.gene065423.t1 | MTR_0400s0030 | 48.485 | 99 | 50 | 1 | 1 | 98 | 791 | 889 | 6.94e-22 | 89.4 |
| MS.gene065423.t1 | MTR_0365s0020 | 48.485 | 99 | 50 | 1 | 1 | 98 | 791 | 889 | 6.94e-22 | 89.4 |
| MS.gene065423.t1 | MTR_7g050990 | 50.515 | 97 | 44 | 2 | 1 | 93 | 819 | 915 | 7.23e-22 | 89.4 |
| MS.gene065423.t1 | MTR_7g009940 | 43.363 | 113 | 59 | 2 | 3 | 114 | 700 | 808 | 1.03e-21 | 89.0 |
| MS.gene065423.t1 | MTR_1g039240 | 46.392 | 97 | 48 | 1 | 1 | 93 | 1058 | 1154 | 1.05e-21 | 89.0 |
| MS.gene065423.t1 | MTR_7g045860 | 48.454 | 97 | 46 | 2 | 2 | 97 | 984 | 1077 | 1.65e-21 | 88.6 |
| MS.gene065423.t1 | MTR_7g446160 | 51.064 | 94 | 45 | 1 | 1 | 93 | 887 | 980 | 1.86e-21 | 88.2 |
| MS.gene065423.t1 | MTR_1g040545 | 48.980 | 98 | 44 | 3 | 1 | 93 | 907 | 1003 | 2.38e-21 | 87.8 |
| MS.gene065423.t1 | MTR_1g040525 | 48.958 | 96 | 43 | 3 | 1 | 91 | 767 | 861 | 3.37e-21 | 87.4 |
| MS.gene065423.t1 | MTR_7g009970 | 43.103 | 116 | 57 | 3 | 1 | 113 | 669 | 778 | 3.69e-21 | 87.4 |
| MS.gene065423.t1 | MTR_1g040615 | 47.423 | 97 | 47 | 3 | 1 | 93 | 2024 | 2120 | 9.19e-21 | 86.3 |
| MS.gene065423.t1 | MTR_1g040615 | 43.636 | 110 | 50 | 5 | 1 | 103 | 1025 | 1129 | 9.92e-19 | 80.5 |
| MS.gene065423.t1 | MTR_7g010000 | 42.241 | 116 | 58 | 3 | 1 | 113 | 746 | 855 | 1.04e-20 | 86.3 |
| MS.gene065423.t1 | MTR_7g007630 | 42.982 | 114 | 60 | 2 | 1 | 113 | 666 | 775 | 3.72e-20 | 84.7 |
| MS.gene065423.t1 | MTR_7g407130 | 42.478 | 113 | 60 | 2 | 1 | 112 | 696 | 804 | 2.76e-19 | 82.0 |
| MS.gene065423.t1 | MTR_1g039220 | 47.368 | 95 | 46 | 3 | 1 | 91 | 1032 | 1126 | 5.73e-19 | 81.3 |
| MS.gene065423.t1 | MTR_7g013680 | 41.026 | 117 | 60 | 3 | 1 | 114 | 619 | 729 | 6.50e-19 | 80.9 |
| MS.gene065423.t1 | MTR_4g037720 | 44.086 | 93 | 48 | 1 | 1 | 93 | 848 | 936 | 1.04e-18 | 80.5 |
| MS.gene065423.t1 | MTR_4g037015 | 43.011 | 93 | 49 | 1 | 1 | 93 | 777 | 865 | 2.35e-18 | 79.3 |
| MS.gene065423.t1 | MTR_7g028432 | 43.011 | 93 | 49 | 1 | 1 | 93 | 853 | 941 | 2.76e-18 | 79.3 |
| MS.gene065423.t1 | MTR_7g407090 | 43.860 | 114 | 59 | 2 | 1 | 113 | 651 | 760 | 6.46e-18 | 78.2 |
| MS.gene065423.t1 | MTR_7g096980 | 40.206 | 97 | 57 | 1 | 1 | 97 | 1108 | 1203 | 1.16e-17 | 77.4 |
| MS.gene065423.t1 | MTR_7g007620 | 44.737 | 114 | 58 | 2 | 1 | 113 | 620 | 729 | 2.06e-17 | 76.6 |
| MS.gene065423.t1 | MTR_7g013350 | 43.689 | 103 | 49 | 3 | 1 | 100 | 295 | 391 | 2.66e-16 | 73.6 |
| MS.gene065423.t1 | MTR_7g446190 | 46.429 | 84 | 38 | 2 | 1 | 80 | 815 | 895 | 9.62e-16 | 72.0 |
| MS.gene065423.t1 | MTR_8g047230 | 50.000 | 92 | 41 | 2 | 1 | 91 | 875 | 962 | 1.28e-15 | 71.6 |
| MS.gene065423.t1 | MTR_0491s0030 | 50.000 | 92 | 41 | 2 | 1 | 91 | 704 | 791 | 2.25e-15 | 70.9 |
| MS.gene065423.t1 | MTR_8g047160 | 51.724 | 87 | 37 | 2 | 1 | 86 | 531 | 613 | 2.47e-15 | 70.9 |
| MS.gene065423.t1 | MTR_7g010010 | 37.069 | 116 | 45 | 3 | 1 | 113 | 616 | 706 | 7.27e-15 | 69.3 |
| MS.gene065423.t1 | MTR_8g047210 | 50.000 | 92 | 41 | 2 | 1 | 91 | 768 | 855 | 3.57e-14 | 67.4 |
| MS.gene065423.t1 | MTR_8g047220 | 50.000 | 92 | 41 | 2 | 1 | 91 | 831 | 918 | 3.76e-14 | 67.4 |
| MS.gene065423.t1 | MTR_2g070020 | 38.000 | 100 | 51 | 2 | 1 | 93 | 987 | 1082 | 8.76e-14 | 66.2 |
| MS.gene065423.t1 | MTR_7g022160 | 36.449 | 107 | 63 | 2 | 1 | 104 | 909 | 1013 | 4.12e-13 | 64.3 |
| MS.gene065423.t1 | MTR_7g022050 | 38.542 | 96 | 54 | 2 | 1 | 93 | 534 | 627 | 1.26e-11 | 60.1 |
| MS.gene065423.t1 | MTR_3g047890 | 36.036 | 111 | 51 | 4 | 3 | 102 | 360 | 461 | 2.16e-11 | 59.7 |
| MS.gene065423.t1 | MTR_1g047670 | 36.000 | 100 | 55 | 3 | 1 | 93 | 989 | 1086 | 2.89e-11 | 59.3 |
| MS.gene065423.t1 | MTR_3g093930 | 34.314 | 102 | 62 | 3 | 1 | 99 | 879 | 978 | 3.13e-11 | 59.3 |
| MS.gene065423.t1 | MTR_4g058710 | 38.384 | 99 | 51 | 3 | 8 | 98 | 419 | 515 | 6.18e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065423.t1 | AT4G08850 | 52.874 | 87 | 36 | 2 | 1 | 86 | 955 | 1037 | 5.63e-19 | 81.3 |
| MS.gene065423.t1 | AT1G35710 | 44.186 | 86 | 44 | 1 | 1 | 86 | 1031 | 1112 | 4.79e-15 | 70.1 |
| MS.gene065423.t1 | AT1G17230 | 38.000 | 100 | 51 | 2 | 1 | 93 | 977 | 1072 | 6.91e-13 | 63.9 |
| MS.gene065423.t1 | AT1G17230 | 38.000 | 100 | 51 | 2 | 1 | 93 | 977 | 1072 | 7.27e-13 | 63.9 |
| MS.gene065423.t1 | AT3G28040 | 34.579 | 107 | 65 | 2 | 1 | 104 | 908 | 1012 | 2.43e-12 | 62.4 |
| MS.gene065423.t1 | AT4G25390 | 37.755 | 98 | 51 | 2 | 3 | 93 | 530 | 624 | 4.73e-12 | 61.6 |
| MS.gene065423.t1 | AT5G51770 | 34.286 | 105 | 58 | 2 | 3 | 99 | 528 | 629 | 1.90e-11 | 59.7 |
| MS.gene065423.t1 | AT4G31110 | 33.645 | 107 | 63 | 3 | 5 | 105 | 634 | 738 | 3.29e-11 | 58.9 |
| MS.gene065423.t1 | AT4G31110 | 33.645 | 107 | 63 | 3 | 5 | 105 | 612 | 716 | 3.45e-11 | 58.9 |
| MS.gene065423.t1 | AT2G33170 | 36.634 | 101 | 52 | 3 | 1 | 93 | 1003 | 1099 | 4.97e-11 | 58.5 |
| MS.gene065423.t1 | AT2G33170 | 36.634 | 101 | 52 | 3 | 1 | 93 | 1003 | 1099 | 4.97e-11 | 58.5 |
Find 23 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGCTTTGGAAATCATAA+AGG | 0.335311 | 7.3:-26117525 | MS.gene065423:CDS |
| AAATGTGATGTATACAGTTT+TGG | 0.382117 | 7.3:-26117551 | MS.gene065423:CDS |
| AAGATAACCTCCTCATCAAT+TGG | 0.413575 | 7.3:+26117389 | None:intergenic |
| CTTGCTTTGGAAATCATAAA+GGG | 0.419654 | 7.3:-26117524 | MS.gene065423:CDS |
| TGGAGCAGGAAAATCACCTT+TGG | 0.448518 | 7.3:-26117288 | MS.gene065423:CDS |
| TTAGCCAATGGCCTTGTAGA+TGG | 0.459178 | 7.3:+26117464 | None:intergenic |
| GTTGGCCGAGAGCATGGCTC+TGG | 0.476657 | 7.3:+26117335 | None:intergenic |
| ATCATAAAGGGAGAACATCC+TGG | 0.483511 | 7.3:-26117512 | MS.gene065423:CDS |
| CAAATTCACACGATCAAACT+AGG | 0.483867 | 7.3:-26117260 | MS.gene065423:CDS |
| TATAATGAAATGAGATCTCC+AGG | 0.486322 | 7.3:+26117494 | None:intergenic |
| GATCAAGTATGTAAGATGCT+TGG | 0.514611 | 7.3:-26117308 | MS.gene065423:CDS |
| GTCACCATCTACAAGGCCAT+TGG | 0.522420 | 7.3:-26117468 | MS.gene065423:CDS |
| GTATGTAAGATGCTTGGAGC+AGG | 0.525016 | 7.3:-26117302 | MS.gene065423:CDS |
| CATCTTACATACTTGATCCA+TGG | 0.528171 | 7.3:+26117313 | None:intergenic |
| TCCATGGTTGGCCGAGAGCA+TGG | 0.533146 | 7.3:+26117329 | None:intergenic |
| TATATTTGTCACCATCTACA+AGG | 0.550499 | 7.3:-26117475 | MS.gene065423:CDS |
| GCCATGCTCTCGGCCAACCA+TGG | 0.570404 | 7.3:-26117330 | MS.gene065423:CDS |
| TTACATACTTGATCCATGGT+TGG | 0.575557 | 7.3:+26117317 | None:intergenic |
| TATGAAACCAATTGATGAGG+AGG | 0.576165 | 7.3:-26117396 | MS.gene065423:CDS |
| GTGTGAATTTGATCTTCCAA+AGG | 0.599159 | 7.3:+26117272 | None:intergenic |
| TAAATCCAGAGCCATGCTCT+CGG | 0.603263 | 7.3:-26117340 | MS.gene065423:CDS |
| AATAATGTATCATTAGCCAA+TGG | 0.628834 | 7.3:+26117452 | None:intergenic |
| AGTTATGAAACCAATTGATG+AGG | 0.632329 | 7.3:-26117399 | MS.gene065423:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATAATGTATCATTAGCCAA+TGG | + | chr7.3:26117361-26117380 | None:intergenic | 25.0% |
| !! | AAATGTGATGTATACAGTTT+TGG | - | chr7.3:26117259-26117278 | MS.gene065423:CDS | 25.0% |
| !!! | ATTGGTTTCATAACTTTTTG+AGG | + | chr7.3:26117406-26117425 | None:intergenic | 25.0% |
| AGTTATGAAACCAATTGATG+AGG | - | chr7.3:26117411-26117430 | MS.gene065423:CDS | 30.0% | |
| TATAATGAAATGAGATCTCC+AGG | + | chr7.3:26117319-26117338 | None:intergenic | 30.0% | |
| TATATTTGTCACCATCTACA+AGG | - | chr7.3:26117335-26117354 | MS.gene065423:CDS | 30.0% | |
| !! | ACTTGCTTTGGAAATCATAA+AGG | - | chr7.3:26117285-26117304 | MS.gene065423:CDS | 30.0% |
| !! | CTTGCTTTGGAAATCATAAA+GGG | - | chr7.3:26117286-26117305 | MS.gene065423:CDS | 30.0% |
| AAGATAACCTCCTCATCAAT+TGG | + | chr7.3:26117424-26117443 | None:intergenic | 35.0% | |
| CAAATTCACACGATCAAACT+AGG | - | chr7.3:26117550-26117569 | MS.gene065423:CDS | 35.0% | |
| CATCTTACATACTTGATCCA+TGG | + | chr7.3:26117500-26117519 | None:intergenic | 35.0% | |
| GATCAAGTATGTAAGATGCT+TGG | - | chr7.3:26117502-26117521 | MS.gene065423:CDS | 35.0% | |
| GTGTGAATTTGATCTTCCAA+AGG | + | chr7.3:26117541-26117560 | None:intergenic | 35.0% | |
| TATGAAACCAATTGATGAGG+AGG | - | chr7.3:26117414-26117433 | MS.gene065423:CDS | 35.0% | |
| TTACATACTTGATCCATGGT+TGG | + | chr7.3:26117496-26117515 | None:intergenic | 35.0% | |
| ATCATAAAGGGAGAACATCC+TGG | - | chr7.3:26117298-26117317 | MS.gene065423:CDS | 40.0% | |
| !!! | CAGTTTTGGAGTACTTGCTT+TGG | - | chr7.3:26117273-26117292 | MS.gene065423:CDS | 40.0% |
| GTATGTAAGATGCTTGGAGC+AGG | - | chr7.3:26117508-26117527 | MS.gene065423:CDS | 45.0% | |
| TAAATCCAGAGCCATGCTCT+CGG | - | chr7.3:26117470-26117489 | MS.gene065423:CDS | 45.0% | |
| TGGAGCAGGAAAATCACCTT+TGG | - | chr7.3:26117522-26117541 | MS.gene065423:CDS | 45.0% | |
| !! | TTAGCCAATGGCCTTGTAGA+TGG | + | chr7.3:26117349-26117368 | None:intergenic | 45.0% |
| GTCACCATCTACAAGGCCAT+TGG | - | chr7.3:26117342-26117361 | MS.gene065423:CDS | 50.0% | |
| ! | TCCATGGTTGGCCGAGAGCA+TGG | + | chr7.3:26117484-26117503 | None:intergenic | 60.0% |
| GCCATGCTCTCGGCCAACCA+TGG | - | chr7.3:26117480-26117499 | MS.gene065423:CDS | 65.0% | |
| ! | GTTGGCCGAGAGCATGGCTC+TGG | + | chr7.3:26117478-26117497 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 26117244 | 26117588 | 26117244 | ID=MS.gene065423 |
| chr7.3 | mRNA | 26117244 | 26117588 | 26117244 | ID=MS.gene065423.t1;Parent=MS.gene065423 |
| chr7.3 | exon | 26117244 | 26117588 | 26117244 | ID=MS.gene065423.t1.exon1;Parent=MS.gene065423.t1 |
| chr7.3 | CDS | 26117244 | 26117588 | 26117244 | ID=cds.MS.gene065423.t1;Parent=MS.gene065423.t1 |
| Gene Sequence |
| Protein sequence |
