AlfalfaGEDB | MS.gene065520

M. sativa cultivar XinJiangDaYe / MS.gene065520

KEGG: -
GO: -
PFAM: PF03195.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065520.t1	XP_003617566.1	97.9	145	3	0	1	145	1	145	1.80E-79	305.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065520.t1	P59467	64.8	91	32	0	5	95	5	95	8.5e-30	131.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065520.t1	G7K3J3	97.9	145	3	0	1	145	1	145	1.3e-79	305.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene065520.t1	TF	LOB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065520.t1	MTR_5g093010	97.931	145	3	0	1	145	1	145	2.41e-106	299
MS.gene065520.t1	MTR_7g028905	82.906	117	20	0	1	117	1	117	1.68e-71	210
MS.gene065520.t1	MTR_5g083230	51.376	109	53	0	6	114	7	115	1.43e-38	129
MS.gene065520.t1	MTR_3g031660	45.833	120	62	1	6	125	8	124	3.59e-37	125
MS.gene065520.t1	MTR_2g068760	48.571	105	54	0	6	110	14	118	4.86e-37	124
MS.gene065520.t1	MTR_3g071590	42.143	140	73	2	6	145	9	140	7.03e-37	124
MS.gene065520.t1	MTR_4g083680	52.525	99	47	0	6	104	16	114	1.82e-35	121
MS.gene065520.t1	MTR_5g083960	45.669	127	66	1	6	129	40	166	8.21e-34	117
MS.gene065520.t1	MTR_3g094690	50.000	106	53	0	6	111	48	153	8.80e-33	115
MS.gene065520.t1	MTR_3g452660	49.074	108	55	0	6	113	8	115	1.49e-32	116
MS.gene065520.t1	MTR_3g073690	45.631	103	56	0	6	108	10	112	4.71e-32	113
MS.gene065520.t1	MTR_4g060950	42.857	112	63	1	1	111	20	131	7.68e-32	112
MS.gene065520.t1	MTR_7g033800	45.690	116	59	1	6	117	12	127	8.17e-32	111
MS.gene065520.t1	MTR_4g105170	47.664	107	56	0	6	112	12	118	8.57e-32	112
MS.gene065520.t1	MTR_2g093310	38.298	141	77	2	1	141	29	159	1.11e-31	112
MS.gene065520.t1	MTR_6g005070	48.000	100	52	0	6	105	10	109	8.02e-31	108
MS.gene065520.t1	MTR_3g094690	50.000	106	52	1	6	111	48	152	9.92e-31	110
MS.gene065520.t1	MTR_6g005080	40.876	137	74	2	6	142	11	140	1.16e-30	107
MS.gene065520.t1	MTR_5g017950	45.631	103	56	0	6	108	9	111	1.18e-30	112
MS.gene065520.t1	MTR_3g077240	49.000	100	51	0	6	105	11	110	1.67e-30	108
MS.gene065520.t1	MTR_5g075020	48.077	104	54	0	6	109	44	147	9.70e-29	105
MS.gene065520.t1	MTR_1g095850	43.564	101	57	0	6	106	73	173	1.06e-28	105
MS.gene065520.t1	MTR_5g075020	48.077	104	54	0	6	109	48	151	1.51e-28	105
MS.gene065520.t1	MTR_6g011250	37.879	132	79	2	6	134	10	141	1.91e-28	103
MS.gene065520.t1	MTR_8g040900	56.250	80	35	0	6	85	8	87	2.68e-28	104
MS.gene065520.t1	MTR_3g071420	44.681	94	52	0	6	99	15	108	2.96e-28	103
MS.gene065520.t1	MTR_6g027710	44.340	106	58	1	6	110	41	146	1.21e-27	102
MS.gene065520.t1	MTR_5g083010	41.584	101	59	0	6	106	12	112	1.27e-27	101
MS.gene065520.t1	MTR_7g096530	45.098	102	55	1	6	106	15	116	3.66e-27	101
MS.gene065520.t1	MTR_6g011230	41.237	97	57	0	6	102	12	108	7.78e-27	99.8
MS.gene065520.t1	MTR_1g070220	43.478	115	60	2	6	119	16	126	1.08e-26	99.8
MS.gene065520.t1	MTR_8g036085	41.667	108	62	1	4	110	22	129	1.44e-25	97.4
MS.gene065520.t1	MTR_7g074990	44.554	101	55	1	6	105	21	121	2.56e-25	96.7
MS.gene065520.t1	MTR_7g096610	41.732	127	67	3	6	125	9	135	9.54e-25	95.1
MS.gene065520.t1	MTR_8g079660	39.604	101	61	0	6	106	9	109	1.02e-24	95.9
MS.gene065520.t1	MTR_8g036105	43.269	104	58	1	4	106	17	120	1.95e-24	94.0
MS.gene065520.t1	MTR_1g070205	37.857	140	78	4	6	144	13	144	4.08e-24	93.6
MS.gene065520.t1	MTR_7g075230	37.063	143	84	4	4	140	11	153	1.05e-23	91.7
MS.gene065520.t1	MTR_8g079580	42.574	101	57	1	6	105	9	109	1.07e-23	92.4
MS.gene065520.t1	MTR_5g080470	41.176	102	59	1	6	106	8	109	2.13e-23	90.9
MS.gene065520.t1	MTR_6g027700	35.878	131	71	2	6	123	8	138	1.57e-22	89.0
MS.gene065520.t1	MTR_8g079620	36.634	101	64	0	6	106	9	109	2.41e-22	89.0
MS.gene065520.t1	MTR_6g011200	35.294	102	65	1	6	106	10	111	3.86e-22	88.6
MS.gene065520.t1	MTR_5g085390	33.333	144	87	4	6	144	10	149	1.18e-21	87.0
MS.gene065520.t1	MTR_4g107450	40.000	100	60	0	6	105	11	110	1.30e-20	85.5
MS.gene065520.t1	MTR_8g017090	36.538	104	66	0	6	109	13	116	3.56e-20	84.3
MS.gene065520.t1	MTR_4g099230	34.314	102	66	1	6	106	3	104	4.13e-20	81.3
MS.gene065520.t1	MTR_2g100020	33.663	101	67	0	6	106	20	120	5.69e-19	80.9
MS.gene065520.t1	MTR_4g088035	31.395	86	59	0	6	91	7	92	2.46e-13	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065520.t1	AT3G26620	64.706	102	36	0	5	106	5	106	1.36e-45	145
MS.gene065520.t1	AT3G26660	64.706	102	36	0	5	106	5	106	1.39e-45	145
MS.gene065520.t1	AT2G30130	50.926	108	53	0	6	113	9	116	9.08e-38	127
MS.gene065520.t1	AT1G31320	60.465	86	34	0	6	91	14	99	5.23e-35	120
MS.gene065520.t1	AT5G63090	42.029	138	80	0	6	143	12	149	1.83e-32	114
MS.gene065520.t1	AT5G63090	42.029	138	80	0	6	143	12	149	1.83e-32	114
MS.gene065520.t1	AT5G63090	42.029	138	80	0	6	143	12	149	1.83e-32	114
MS.gene065520.t1	AT5G63090	42.029	138	80	0	6	143	12	149	1.83e-32	114
MS.gene065520.t1	AT3G27650	49.000	100	51	0	6	105	40	139	3.06e-32	112
MS.gene065520.t1	AT2G28500	42.342	111	64	0	1	111	51	161	1.19e-31	113
MS.gene065520.t1	AT1G07900	42.342	111	64	0	1	111	29	139	1.32e-31	111
MS.gene065520.t1	AT1G16530	49.505	101	50	1	6	105	15	115	1.58e-31	110
MS.gene065520.t1	AT2G30340	47.170	106	56	0	6	111	53	158	2.29e-31	113
MS.gene065520.t1	AT2G30340	47.170	106	56	0	6	111	54	159	2.39e-31	113
MS.gene065520.t1	AT2G23660	45.631	103	56	0	6	108	6	108	6.54e-31	113
MS.gene065520.t1	AT2G23660	45.631	103	56	0	6	108	6	108	6.54e-31	113
MS.gene065520.t1	AT2G23660	45.631	103	56	0	6	108	6	108	6.54e-31	113
MS.gene065520.t1	AT5G66870	44.660	103	57	0	6	108	8	110	9.83e-31	112
MS.gene065520.t1	AT2G40470	45.385	130	65	2	6	135	21	144	2.83e-30	108
MS.gene065520.t1	AT2G40470	45.736	129	64	2	6	134	46	168	4.43e-30	108
MS.gene065520.t1	AT3G11090	35.294	136	80	1	6	141	12	139	1.08e-28	103
MS.gene065520.t1	AT1G65620	45.545	101	55	0	6	106	10	110	5.32e-28	102
MS.gene065520.t1	AT1G65620	45.545	101	55	0	6	106	10	110	5.32e-28	102
MS.gene065520.t1	AT1G65620	45.545	101	55	0	6	106	10	110	5.32e-28	102
MS.gene065520.t1	AT1G65620	45.545	101	55	0	6	106	10	110	5.32e-28	102
MS.gene065520.t1	AT1G65620	45.545	101	55	0	6	106	10	110	5.32e-28	102
MS.gene065520.t1	AT4G00210	39.416	137	73	3	4	132	10	144	1.11e-27	102
MS.gene065520.t1	AT3G03760	45.545	101	54	1	6	105	52	152	4.48e-27	102
MS.gene065520.t1	AT2G42430	43.810	105	58	1	6	109	16	120	1.17e-26	100
MS.gene065520.t1	AT3G50510	41.905	105	61	0	2	106	9	113	3.76e-26	98.2
MS.gene065520.t1	AT3G50510	41.905	105	61	0	2	106	9	113	3.76e-26	98.2
MS.gene065520.t1	AT5G06080	41.322	121	67	2	6	125	8	125	1.99e-25	95.5
MS.gene065520.t1	AT2G45410	45.000	100	54	1	8	106	19	118	7.82e-25	94.4
MS.gene065520.t1	AT3G58190	43.137	102	57	1	6	106	12	113	5.46e-24	92.8
MS.gene065520.t1	AT4G00220	40.385	104	61	1	8	110	20	123	5.73e-24	93.2
MS.gene065520.t1	AT2G42440	43.000	100	56	1	6	104	8	107	7.23e-24	93.2
MS.gene065520.t1	AT3G47870	37.736	106	66	0	4	109	35	140	2.56e-23	93.2
MS.gene065520.t1	AT2G45420	42.308	104	59	1	8	110	40	143	3.50e-23	91.7
MS.gene065520.t1	AT3G13850	33.766	154	82	2	6	139	37	190	1.08e-22	90.5
MS.gene065520.t1	AT5G35900	40.777	103	54	1	6	101	6	108	1.25e-20	84.0
MS.gene065520.t1	AT2G31310	31.206	141	89	3	6	145	8	141	2.96e-20	82.4
MS.gene065520.t1	AT1G72980	33.077	130	83	2	6	134	14	140	8.60e-18	76.6
MS.gene065520.t1	AT1G06280	37.209	86	54	0	6	91	25	110	1.05e-17	76.3
MS.gene065520.t1	AT2G19820	34.021	97	58	2	6	101	13	104	5.70e-15	67.0
MS.gene065520.t1	AT4G22700	31.579	114	76	2	1	113	1	113	2.57e-13	64.3
MS.gene065520.t1	AT5G15060	43.243	74	38	2	6	76	18	90	8.09e-13	62.0
MS.gene065520.t1	AT1G36000	27.957	93	67	0	6	98	10	102	7.92e-12	58.9

Find 33 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGCTTCATATTGTTGATTT+TGG	0.095309	5.2:+78395062	None:intergenic
AGGATTCAAAATCCTGTTTA+TGG	0.168254	5.2:-78395178	MS.gene065520:CDS
AGCAAATCTTTGAGGATTAT+TGG	0.230583	5.2:+78395440	None:intergenic
AGCAATCTGAGTTTGGATTT+TGG	0.249357	5.2:+78395096	None:intergenic
GGATTATTGGCAGGGAAATA+TGG	0.290635	5.2:+78395453	None:intergenic
GGCCCAATCAAACACCTAAT+TGG	0.314557	5.2:-78394985	MS.gene065520:CDS
GCTTCTGTCCACCGAATCTA+TGG	0.338967	5.2:-78395420	MS.gene065520:CDS
TATGACAAGCAATCTGAGTT+TGG	0.340516	5.2:+78395089	None:intergenic
GAACCAATTAGGTGTTTGAT+TGG	0.385252	5.2:+78394982	None:intergenic
AAGTCTCAATTGAACCAATT+AGG	0.390330	5.2:+78394971	None:intergenic
CCAGCTCAGCTTCTGTATCA+TGG	0.391779	5.2:+78395119	None:intergenic
ATCCCAACACATCCATAAAC+AGG	0.441067	5.2:+78395166	None:intergenic
AATCTTTGAGGATTATTGGC+AGG	0.455276	5.2:+78395444	None:intergenic
TGCTCCATGCTGCTACTTTG+TGG	0.458851	5.2:+78395016	None:intergenic
AACCAATTAGGTGTTTGATT+GGG	0.459934	5.2:+78394983	None:intergenic
CCATGATACAGAAGCTGAGC+TGG	0.490473	5.2:-78395119	MS.gene065520:CDS
TGTATTTAGAAGCACAATGT+AGG	0.494601	5.2:-78395198	MS.gene065520:CDS
TGGACAGAAGCAAATCTTTG+AGG	0.503992	5.2:+78395432	None:intergenic
CAAAATCAACAATATGAAGC+TGG	0.506425	5.2:-78395061	MS.gene065520:CDS
ATCTATGGTGGTGCCAATGT+TGG	0.519520	5.2:-78395405	MS.gene065520:CDS
CATTGGCACCACCATAGATT+CGG	0.530883	5.2:+78395409	None:intergenic
TTGACATCATGATTTATGGT+AGG	0.535888	5.2:-78395530	None:intergenic
AATCCTGTTTATGGATGTGT+TGG	0.538555	5.2:-78395169	MS.gene065520:CDS
GGAGAGAAAATGCAATCAGA+AGG	0.551169	5.2:+78395474	None:intergenic
CTTGCAAGAGTCAAAGAAGA+AGG	0.555730	5.2:-78395500	MS.gene065520:CDS
ATCTTTGAGGATTATTGGCA+GGG	0.557843	5.2:+78395445	None:intergenic
GCCTCCACAAAGTAGCAGCA+TGG	0.580606	5.2:-78395020	MS.gene065520:CDS
ATCCTGTTTATGGATGTGTT+GGG	0.594258	5.2:-78395168	MS.gene065520:CDS
GCTTGTTCTCTTACATAGTG+AGG	0.601870	5.2:+78395232	None:intergenic
GAGAGAAAATGCAATCAGAA+GGG	0.619697	5.2:+78395475	None:intergenic
TGGCACCACCATAGATTCGG+TGG	0.632816	5.2:+78395412	None:intergenic
TCTGTCCACCGAATCTATGG+TGG	0.637607	5.2:-78395417	MS.gene065520:CDS
TCCATGCTGCTACTTTGTGG+AGG	0.665689	5.2:+78395019	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTAATATAATTATAACATA+TGG	-	chr5.2:78395175-78395194	MS.gene065520:CDS	10.0%
!!!	TTCTTATCTAGTATTTTTAT+TGG	-	chr5.2:78395232-78395251	MS.gene065520:CDS	15.0%
	AACCAATTAGGTGTTTGATT+GGG	+	chr5.2:78395518-78395537	None:intergenic	30.0%
	AGGATTCAAAATCCTGTTTA+TGG	-	chr5.2:78395320-78395339	MS.gene065520:intron	30.0%
	CAAAATCAACAATATGAAGC+TGG	-	chr5.2:78395437-78395456	MS.gene065520:CDS	30.0%
!	AGCAAATCTTTGAGGATTAT+TGG	+	chr5.2:78395061-78395080	None:intergenic	30.0%
!	TGTATTTAGAAGCACAATGT+AGG	-	chr5.2:78395300-78395319	MS.gene065520:intron	30.0%
!!!	CAGCTTCATATTGTTGATTT+TGG	+	chr5.2:78395439-78395458	None:intergenic	30.0%
	AATCCTGTTTATGGATGTGT+TGG	-	chr5.2:78395329-78395348	MS.gene065520:intron	35.0%
	ATCCTGTTTATGGATGTGTT+GGG	-	chr5.2:78395330-78395349	MS.gene065520:intron	35.0%
	CAATGTTGGAAAAATGCTTC+AGG	-	chr5.2:78395107-78395126	MS.gene065520:CDS	35.0%
	GAACCAATTAGGTGTTTGAT+TGG	+	chr5.2:78395519-78395538	None:intergenic	35.0%
	GAGAGAAAATGCAATCAGAA+GGG	+	chr5.2:78395026-78395045	None:intergenic	35.0%
	TATGACAAGCAATCTGAGTT+TGG	+	chr5.2:78395412-78395431	None:intergenic	35.0%
!	AATCTTTGAGGATTATTGGC+AGG	+	chr5.2:78395057-78395076	None:intergenic	35.0%
!	AGCAATCTGAGTTTGGATTT+TGG	+	chr5.2:78395405-78395424	None:intergenic	35.0%
!	ATCTTTGAGGATTATTGGCA+GGG	+	chr5.2:78395056-78395075	None:intergenic	35.0%
!!	CTGAAGCATTTTTCCAACAT+TGG	+	chr5.2:78395109-78395128	None:intergenic	35.0%
	ATCCCAACACATCCATAAAC+AGG	+	chr5.2:78395335-78395354	None:intergenic	40.0%
	CTTGCAAGAGTCAAAGAAGA+AGG	-	chr5.2:78394998-78395017	MS.gene065520:CDS	40.0%
	GCTTGTTCTCTTACATAGTG+AGG	+	chr5.2:78395269-78395288	None:intergenic	40.0%
	GGAGAGAAAATGCAATCAGA+AGG	+	chr5.2:78395027-78395046	None:intergenic	40.0%
	GGATTATTGGCAGGGAAATA+TGG	+	chr5.2:78395048-78395067	None:intergenic	40.0%
	TGGACAGAAGCAAATCTTTG+AGG	+	chr5.2:78395069-78395088	None:intergenic	40.0%
	CATTGGCACCACCATAGATT+CGG	+	chr5.2:78395092-78395111	None:intergenic	45.0%
	GGCCCAATCAAACACCTAAT+TGG	-	chr5.2:78395513-78395532	MS.gene065520:CDS	45.0%
!	ATCTATGGTGGTGCCAATGT+TGG	-	chr5.2:78395093-78395112	MS.gene065520:CDS	45.0%
!!	GCAGCATGGAGCATTTTCAA+TGG	-	chr5.2:78395492-78395511	MS.gene065520:CDS	45.0%
	CCAGCTCAGCTTCTGTATCA+TGG	+	chr5.2:78395382-78395401	None:intergenic	50.0%
	CCATGATACAGAAGCTGAGC+TGG	-	chr5.2:78395379-78395398	MS.gene065520:intron	50.0%
	GCTTCTGTCCACCGAATCTA+TGG	-	chr5.2:78395078-78395097	MS.gene065520:CDS	50.0%
	TCTGTCCACCGAATCTATGG+TGG	-	chr5.2:78395081-78395100	MS.gene065520:CDS	50.0%
!!	TCCATGCTGCTACTTTGTGG+AGG	+	chr5.2:78395482-78395501	None:intergenic	50.0%
!!	TGCTCCATGCTGCTACTTTG+TGG	+	chr5.2:78395485-78395504	None:intergenic	50.0%
	GCCTCCACAAAGTAGCAGCA+TGG	-	chr5.2:78395478-78395497	MS.gene065520:CDS	55.0%
	TGGCACCACCATAGATTCGG+TGG	+	chr5.2:78395089-78395108	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	78394976	78395544	78394976	ID=MS.gene065520
chr5.2	mRNA	78394976	78395544	78394976	ID=MS.gene065520.t1;Parent=MS.gene065520
chr5.2	exon	78395392	78395544	78395392	ID=MS.gene065520.t1.exon1;Parent=MS.gene065520.t1
chr5.2	CDS	78395392	78395544	78395392	ID=cds.MS.gene065520.t1;Parent=MS.gene065520.t1
chr5.2	exon	78394976	78395260	78394976	ID=MS.gene065520.t1.exon2;Parent=MS.gene065520.t1
chr5.2	CDS	78394976	78395260	78394976	ID=cds.MS.gene065520.t1;Parent=MS.gene065520.t1

Gene Sequence

>MS.gene065520

ATGATTTATGGTAGGTGTGCTGCTTGCAAGAGTCAAAGAAGAAGGTGCCCTTCTGATTGCATTTTCTCTCCATATTTCCCTGCCAATAATCCTCAAAGATTTGCTTCTGTCCACCGAATCTATGGTGGTGCCAATGTTGGAAAAATGCTTCAGGTACTTTTAATAATGTATTGATATTTAGTATCTAGTATTTATATTATGTAATATAATTATAACATATGGAAAGAATATCAAAGTTATAAAAAATATATGTTTTTTCTTATCTAGTATTTTTATTGGTGCAGCAATTGCCTCACTATGTAAGAGAACAAGCTGCAAATACTTTGTATTTAGAAGCACAATGTAGGATTCAAAATCCTGTTTATGGATGTGTTGGGATCATCTCTAAATTGTATCAACAAATCCATGATACAGAAGCTGAGCTGGCCAAAATCCAAACTCAGATTGCTTGTCATAAGCTCCAAAATCAACAATATGAAGCTGGATCAAACTTCAATCTTTTGCCTCCACAAAGTAGCAGCATGGAGCATTTTCAATGGCCCAATCAAACACCTAATTGGTTCAATTGA

Protein sequence

>MS.gene065520.t1

MIYGRCAACKSQRRRCPSDCIFSPYFPANNPQRFASVHRIYGGANVGKMLQQLPHYVREQAANTLYLEAQCRIQNPVYGCVGIISKLYQQIHDTEAELAKIQTQIACHKLQNQQYEAGSNFNLLPPQSSSMEHFQWPNQTPNWFN