AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065717

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065717.t1 MTR_1g058350 98.033 305 5 1 1 305 20 323 0.0 625
MS.gene065717.t1 MTR_7g105370 60.465 258 66 10 60 305 64 297 8.19e-87 262
MS.gene065717.t1 MTR_8g023990 70.796 113 29 2 52 162 167 277 2.06e-48 169
MS.gene065717.t1 MTR_5g053920 66.087 115 33 1 53 161 191 305 2.35e-45 158
MS.gene065717.t1 MTR_7g117455 69.725 109 29 2 56 162 117 223 2.78e-45 159
MS.gene065717.t1 MTR_1g093600 61.157 121 36 2 53 162 188 308 9.27e-41 146
MS.gene065717.t1 MTR_1g116920 50.467 107 43 2 45 146 145 246 1.02e-28 112
MS.gene065717.t1 MTR_7g073240 38.571 140 64 7 31 162 98 223 2.80e-16 78.6
MS.gene065717.t1 MTR_5g075410 36.296 135 65 6 35 162 302 422 3.51e-15 76.3
MS.gene065717.t1 MTR_4g028130 35.338 133 66 6 35 160 295 414 3.95e-15 76.3
MS.gene065717.t1 MTR_4g028130 35.338 133 66 6 35 160 295 414 5.00e-15 75.9
MS.gene065717.t1 MTR_4g028130 34.815 135 68 6 35 162 295 416 6.79e-15 75.5
MS.gene065717.t1 MTR_3g467380 35.758 165 81 9 35 188 319 469 2.46e-14 73.9
MS.gene065717.t1 MTR_2g011940 36.667 120 62 5 43 160 336 443 2.75e-14 73.6
MS.gene065717.t1 MTR_2g011940 36.667 120 62 5 43 160 345 452 5.70e-14 72.8
MS.gene065717.t1 MTR_2g011940 36.066 122 64 5 43 162 345 454 5.72e-14 72.8
MS.gene065717.t1 MTR_8g101360 38.053 113 64 3 48 160 129 235 1.04e-12 68.9
MS.gene065717.t1 MTR_3g073420 35.897 117 69 4 46 160 105 217 1.01e-11 65.9
MS.gene065717.t1 MTR_1g094960 31.293 147 81 4 52 197 113 240 1.47e-11 65.5
MS.gene065717.t1 MTR_2g094570 36.697 109 66 2 52 160 103 208 1.72e-11 65.1
MS.gene065717.t1 MTR_2g094570 33.813 139 84 3 52 185 103 238 1.83e-11 65.1
MS.gene065717.t1 MTR_2g094570 36.697 109 66 2 52 160 159 264 1.99e-11 65.1
MS.gene065717.t1 MTR_2g093740 29.630 162 108 3 56 217 167 322 2.26e-11 64.7
MS.gene065717.t1 MTR_4g060460 30.178 169 112 3 49 217 158 320 2.40e-11 64.7
MS.gene065717.t1 MTR_4g060460 30.178 169 112 3 49 217 158 320 2.45e-11 64.7
MS.gene065717.t1 MTR_2g093740 29.630 162 108 3 56 217 167 322 2.66e-11 64.7
MS.gene065717.t1 MTR_2g093740 29.630 162 108 3 56 217 37 192 2.71e-11 64.7
MS.gene065717.t1 MTR_2g093740 29.630 162 108 3 56 217 167 322 2.94e-11 64.3
MS.gene065717.t1 MTR_4g058930 32.432 148 83 4 52 194 104 239 3.14e-11 64.3
MS.gene065717.t1 MTR_4g058930 32.432 148 83 4 52 194 104 239 3.15e-11 64.3
MS.gene065717.t1 MTR_2g093740 29.630 162 108 3 56 217 37 192 3.31e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065717.t1 AT5G06250 51.786 224 81 4 50 265 39 243 1.41e-64 204
MS.gene065717.t1 AT5G06250 52.727 220 77 4 50 261 39 239 1.64e-64 204
MS.gene065717.t1 AT5G06250 52.727 220 77 4 50 261 39 239 2.09e-64 204
MS.gene065717.t1 AT3G11580 52.968 219 73 7 52 260 24 222 3.78e-62 198
MS.gene065717.t1 AT3G11580 52.968 219 73 7 52 260 24 222 3.90e-61 199
MS.gene065717.t1 AT5G06250 51.802 222 65 5 50 263 39 226 8.85e-61 194
MS.gene065717.t1 AT5G06250 51.802 222 65 5 50 263 39 226 1.26e-60 194
MS.gene065717.t1 AT2G36080 49.780 227 63 3 51 276 32 208 5.38e-59 189
MS.gene065717.t1 AT2G36080 49.561 228 64 3 51 277 32 209 5.98e-59 189
MS.gene065717.t1 AT3G11580 76.471 119 19 2 52 161 24 142 2.95e-58 187
MS.gene065717.t1 AT3G11580 76.471 119 19 2 52 161 24 142 2.95e-58 187
MS.gene065717.t1 AT3G11580 76.471 119 19 2 52 161 24 142 2.95e-58 187
MS.gene065717.t1 AT2G36080 78.571 112 23 1 51 161 32 143 6.38e-56 179
MS.gene065717.t1 AT2G36080 78.571 112 23 1 51 161 32 143 6.38e-56 179
MS.gene065717.t1 AT3G61970 43.805 226 91 6 52 260 18 224 1.16e-50 169
MS.gene065717.t1 AT1G01030 43.882 237 100 11 46 260 45 270 9.43e-48 163
MS.gene065717.t1 AT2G46870 69.912 113 29 2 52 161 30 140 1.01e-47 162
MS.gene065717.t1 AT1G68840 63.934 122 44 0 42 163 173 294 2.06e-46 160
MS.gene065717.t1 AT1G68840 63.934 122 44 0 42 163 173 294 2.06e-46 160
MS.gene065717.t1 AT1G13260 66.087 115 38 1 54 168 185 298 2.45e-46 159
MS.gene065717.t1 AT3G25730 62.097 124 45 2 54 175 180 303 2.16e-45 157
MS.gene065717.t1 AT1G01030 62.185 119 40 2 46 161 45 161 1.91e-44 155
MS.gene065717.t1 AT1G25560 59.375 128 46 1 53 174 191 318 7.40e-44 154
MS.gene065717.t1 AT4G01500 56.198 121 40 2 51 162 30 146 1.22e-37 136
MS.gene065717.t1 AT1G50680 50.000 98 40 1 56 144 156 253 8.09e-26 105
MS.gene065717.t1 AT1G51120 49.474 95 42 1 56 144 177 271 3.16e-25 103
MS.gene065717.t1 AT2G30470 35.766 137 76 5 56 187 294 423 2.17e-15 77.0
MS.gene065717.t1 AT4G32010 31.250 144 90 4 56 197 285 421 2.64e-14 73.9
MS.gene065717.t1 AT4G32010 31.944 144 89 5 56 197 281 417 3.10e-14 73.6
MS.gene065717.t1 AT1G77850 36.283 113 65 3 50 160 112 219 2.76e-12 67.4
MS.gene065717.t1 AT1G77850 36.283 113 65 3 50 160 112 219 3.08e-12 67.4
MS.gene065717.t1 AT1G35240 33.793 145 87 4 41 178 105 247 1.06e-11 65.9
MS.gene065717.t1 AT1G35520 33.103 145 88 4 41 178 105 247 1.76e-11 65.1
MS.gene065717.t1 AT1G34310 32.414 145 89 4 41 178 105 247 2.61e-11 64.7
MS.gene065717.t1 AT5G60450 31.288 163 103 4 56 217 152 306 5.81e-11 63.5
MS.gene065717.t1 AT5G60450 31.288 163 103 4 56 217 176 330 6.37e-11 63.5
MS.gene065717.t1 AT2G33860 30.000 160 106 3 56 215 158 311 8.69e-11 63.2
MS.gene065717.t1 AT2G33860 30.000 160 106 3 56 215 1 154 8.71e-11 62.8

Find 76 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATAGTACCTTGTGTTAA+TGG 0.230993 1.2:-45824971 None:intergenic
CAAGGGTACTTAGGTTGTTT+GGG 0.235789 1.2:+45824873 MS.gene065717:CDS
TCAAGGGTACTTAGGTTGTT+TGG 0.238957 1.2:+45824872 MS.gene065717:CDS
TTCCCACCGGTTTCGTCCTT+TGG 0.253544 1.2:-45824826 None:intergenic
CGTCCTTTGGTTCTGACTTT+GGG 0.260868 1.2:-45824813 None:intergenic
TATTAGTCGAACCAACTTCT+TGG 0.317183 1.2:+45822914 MS.gene065717:CDS
GGCGTTTCCGTTACTCATAT+TGG 0.359426 1.2:+45823232 MS.gene065717:CDS
TCGTCCTTTGGTTCTGACTT+TGG 0.367217 1.2:-45824814 None:intergenic
CCTACGTCAGAAGGTGTTAA+GGG 0.377142 1.2:-45823087 None:intergenic
GGTAAGAATGAGGTTGTTAA+TGG 0.377492 1.2:+45823462 MS.gene065717:CDS
CTTCTAGTTCAAGGGTACTT+AGG 0.380189 1.2:+45824864 MS.gene065717:CDS
CAAGTTCAACTTCTAGTTCA+AGG 0.381802 1.2:+45824855 MS.gene065717:CDS
GGGTGAGAATTAGAATTAGA+AGG 0.383626 1.2:-45825013 None:intergenic
TCCTACGTCAGAAGGTGTTA+AGG 0.388480 1.2:-45823088 None:intergenic
AGAAGGTTGATGATATTGAT+AGG 0.411150 1.2:-45824996 None:intergenic
CAAAGCTATGTTCTAACAAA+AGG 0.431965 1.2:+45823264 MS.gene065717:CDS
TGATGATGATGCGCAGGATA+AGG 0.435593 1.2:-45823522 None:intergenic
GGTGTTGAAGAATCATGTTC+AGG 0.437272 1.2:-45824914 None:intergenic
AATAAAACCCGAAGACGAAA+AGG 0.463997 1.2:+45823047 MS.gene065717:CDS
TCAACTGGAGGAGGAGGAGT+GGG 0.485693 1.2:+45823376 MS.gene065717:CDS
AAAGACAAACGACTCGATGC+TGG 0.489099 1.2:+45823303 MS.gene065717:CDS
AATGAGGTTGTTAATGGTGT+AGG 0.496825 1.2:+45823468 MS.gene065717:CDS
ACCATGGAGGTTGGTGGTGA+TGG 0.501172 1.2:-45822942 None:intergenic
TCAGAACCAAAGGACGAAAC+CGG 0.504470 1.2:+45824820 MS.gene065717:CDS
ATCAACTGGAGGAGGAGGAG+TGG 0.505623 1.2:+45823375 MS.gene065717:CDS
AGCAGAGGGGTGGGACATCA+TGG 0.518191 1.2:+45823420 MS.gene065717:CDS
CATCATGGTAAAGGTAGTGA+TGG 0.524393 1.2:+45823435 MS.gene065717:CDS
GAGGAGTGGGGATGATCAGT+CGG 0.525500 1.2:+45823389 MS.gene065717:CDS
GCTATGTTCTAACAAAAGGT+TGG 0.532394 1.2:+45823268 MS.gene065717:CDS
AAGTTCAACTTCTAGTTCAA+GGG 0.539417 1.2:+45824856 MS.gene065717:CDS
AAGGGTACTTAGGTTGTTTG+GGG 0.543731 1.2:+45824874 MS.gene065717:CDS
TAGTAAAAGTCCTTTGCACT+CGG 0.550197 1.2:-45823181 None:intergenic
ATGGTGTAGGGTGGTCCAGA+GGG 0.553370 1.2:+45823481 MS.gene065717:CDS
AAGATGAGTCAGGGAAGTGT+TGG 0.556819 1.2:+45823211 MS.gene065717:CDS
GAACTTGAACTCTTTCCCAC+CGG 0.558795 1.2:-45824839 None:intergenic
TCCATCACCACCAACCTCCA+TGG 0.565376 1.2:+45822941 MS.gene065717:CDS
AATGGTGTAGGGTGGTCCAG+AGG 0.566906 1.2:+45823480 MS.gene065717:CDS
GCGGAGTCTGCTCCGCCGAG+TGG 0.567487 1.2:-45823159 None:intergenic
CATGATCAATGTCTTCATGC+AGG 0.570998 1.2:+45823561 MS.gene065717:CDS
GCAGACTCCGCCGAGTGCAA+AGG 0.576404 1.2:+45823171 MS.gene065717:CDS
TACTGTTCCAATATGAGTAA+CGG 0.578342 1.2:-45823239 None:intergenic
TTGAAGAATCATGTTCAGGT+TGG 0.578477 1.2:-45824910 None:intergenic
TAGGTTGTTTGGGGTCAACA+TGG 0.578736 1.2:+45824883 MS.gene065717:CDS
ATGCGCAGAATAGAACCCTC+TGG 0.580439 1.2:-45823496 None:intergenic
GGCGTATGTTAGCAGAGGGG+TGG 0.580643 1.2:+45823410 MS.gene065717:CDS
TGATGGAGAGACCAAGAAGT+TGG 0.584201 1.2:-45822925 None:intergenic
GCGTATGTTAGCAGAGGGGT+GGG 0.587165 1.2:+45823411 MS.gene065717:CDS
GGGGTGGGACATCATGGTAA+AGG 0.589571 1.2:+45823426 MS.gene065717:CDS
AGACATTGATCATGTTGGTA+TGG 0.589887 1.2:-45823552 None:intergenic
ATGAGGTTGTTAATGGTGTA+GGG 0.597680 1.2:+45823469 MS.gene065717:CDS
GAGAATTGAACCATGGAGGT+TGG 0.598304 1.2:-45822951 None:intergenic
AAGACTTGATGATGATGCGC+AGG 0.600336 1.2:-45823528 None:intergenic
GTTATAATGTTACAGGTAGA+GGG 0.600635 1.2:+45824789 MS.gene065717:intron
ATCATGGTAAAGGTAGTGAT+GGG 0.605307 1.2:+45823436 MS.gene065717:CDS
TTACTATCATAAGAACATTG+AGG 0.605601 1.2:-45824935 None:intergenic
TGGATTTGAGAATTGAACCA+TGG 0.606350 1.2:-45822958 None:intergenic
TGATGGGAATGGTAAGAATG+AGG 0.609090 1.2:+45823452 MS.gene065717:CDS
CATGAAGACATTGATCATGT+TGG 0.609605 1.2:-45823557 None:intergenic
GGTCCCAAAGTCAGAACCAA+AGG 0.613585 1.2:+45824810 MS.gene065717:CDS
GGTAAAGGTAGTGATGGGAA+TGG 0.616121 1.2:+45823441 MS.gene065717:CDS
AGTCGGCGTATGTTAGCAGA+GGG 0.627715 1.2:+45823406 MS.gene065717:CDS
GAACCAAAGGACGAAACCGG+TGG 0.630994 1.2:+45824823 MS.gene065717:CDS
CAGTCGGCGTATGTTAGCAG+AGG 0.631740 1.2:+45823405 MS.gene065717:CDS
CCCTTAACACCTTCTGACGT+AGG 0.645250 1.2:+45823087 MS.gene065717:CDS
AACCAAAGGACGAAACCGGT+GGG 0.646214 1.2:+45824824 MS.gene065717:CDS
TAAAAGTCCTTTGCACTCGG+CGG 0.651002 1.2:-45823178 None:intergenic
CAACTGGAGGAGGAGGAGTG+GGG 0.652199 1.2:+45823377 MS.gene065717:CDS
GGTTGGTGACGTACCACGTG+AGG 0.657771 1.2:-45825034 None:intergenic
AATTGAACCATGGAGGTTGG+TGG 0.658505 1.2:-45822948 None:intergenic
TTCTAATTCTCACCCTCACG+TGG 0.665652 1.2:+45825021 MS.gene065717:CDS
GTTGGTGACGTACCACGTGA+GGG 0.676010 1.2:-45825033 None:intergenic
GTTGGTTCGACTAATAACAT+CGG 0.683554 1.2:-45822907 None:intergenic
AATAATATGCCATTAACACA+AGG 0.689101 1.2:+45824962 MS.gene065717:CDS
ATTTGAGAATTGAACCATGG+AGG 0.715955 1.2:-45822955 None:intergenic
AGGTTGTTAATGGTGTAGGG+TGG 0.756848 1.2:+45823472 MS.gene065717:CDS
GTCGGCGTATGTTAGCAGAG+GGG 0.766314 1.2:+45823407 MS.gene065717:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAATTATATATTTATA+TGG - chr1.2:45823698-45823717 None:intergenic 0.0%
!! AAGAAAAAAAGAAAAAAAAA+AGG - chr1.2:45824163-45824182 None:intergenic 10.0%
!! AATATGATTATTATTATCAT+AGG + chr1.2:45824743-45824762 MS.gene065717:intron 10.0%
!! TTTCAATAATAAAAGTTTAT+AGG + chr1.2:45824587-45824606 MS.gene065717:intron 10.0%
!!! TGTCTTTTTTTAATTATATT+AGG + chr1.2:45825110-45825129 MS.gene065717:intron 10.0%
!! TCTATGAAAAAATTATAACA+AGG + chr1.2:45824255-45824274 MS.gene065717:intron 15.0%
!!! CTTCTTTGTTTTTTTTTTTT+TGG - chr1.2:45824344-45824363 None:intergenic 15.0%
!!! TTAGTACTTAGATTTTTTTA+GGG - chr1.2:45823671-45823690 None:intergenic 15.0%
!! ACATATATCTAGTAGTAATA+TGG - chr1.2:45825058-45825077 None:intergenic 20.0%
!! ATGAAACAATTATATTCTCA+TGG + chr1.2:45824364-45824383 MS.gene065717:intron 20.0%
!! ATTTGCATACAAACAAAATA+AGG - chr1.2:45824709-45824728 None:intergenic 20.0%
!! CTCATAAAATGAAAGAAAAA+AGG - chr1.2:45824107-45824126 None:intergenic 20.0%
!! GAAAGAAAAAAGGAAAAATA+TGG - chr1.2:45824097-45824116 None:intergenic 20.0%
!! GTAAAGATTAATTGTCAAAT+TGG - chr1.2:45823990-45824009 None:intergenic 20.0%
!! TTTGCATACAAACAAAATAA+GGG - chr1.2:45824708-45824727 None:intergenic 20.0%
!!! AAAAGGAAAAATATGGTTTT+AGG - chr1.2:45824090-45824109 None:intergenic 20.0%
!!! AAAGGAAAAATATGGTTTTA+GGG - chr1.2:45824089-45824108 None:intergenic 20.0%
!!! ATTATAATAGCATGGTTTTT+CGG - chr1.2:45823963-45823982 None:intergenic 20.0%
!!! GTCACATTTTTCTTATTTTA+CGG + chr1.2:45824681-45824700 MS.gene065717:intron 20.0%
!!! GTTAGTACTTAGATTTTTTT+AGG - chr1.2:45823672-45823691 None:intergenic 20.0%
!!! TTGATTGATAGTACTTAAAT+GGG + chr1.2:45823741-45823760 MS.gene065717:intron 20.0%
!!! TTTTTCATAGAAAAAAGAAC+AGG - chr1.2:45824246-45824265 None:intergenic 20.0%
! AAGAAAACAGATGAAAACAA+AGG - chr1.2:45824463-45824482 None:intergenic 25.0%
! AATAATATGCCATTAACACA+AGG + chr1.2:45824962-45824981 MS.gene065717:CDS 25.0%
! AGATTAATTGTCAAATTGGA+TGG - chr1.2:45823986-45824005 None:intergenic 25.0%
! ATATCTAGTAGTAATATGGT+TGG - chr1.2:45825054-45825073 None:intergenic 25.0%
! ATCAATCAAGTAGTTGAATT+AGG - chr1.2:45823730-45823749 None:intergenic 25.0%
! TCAATCAAGTAGTTGAATTA+GGG - chr1.2:45823729-45823748 None:intergenic 25.0%
! TTACTATCATAAGAACATTG+AGG - chr1.2:45824938-45824957 None:intergenic 25.0%
! TTCAAAATTCGTTTCTGATT+AGG - chr1.2:45824659-45824678 None:intergenic 25.0%
! TTGCATACAAACAAAATAAG+GGG - chr1.2:45824707-45824726 None:intergenic 25.0%
!! CTTGATTGATAGTACTTAAA+TGG + chr1.2:45823740-45823759 MS.gene065717:intron 25.0%
!! TTCTTGAACAAGAATTTTGT+CGG + chr1.2:45823826-45823845 MS.gene065717:intron 25.0%
!! TTTCTTTCATTTTATGAGTG+TGG + chr1.2:45824109-45824128 MS.gene065717:intron 25.0%
!!! AACTCGATAATTAGTCTTTA+CGG + chr1.2:45823865-45823884 MS.gene065717:intron 25.0%
!!! ATTTTTCAGCGTATTGTTTT+GGG - chr1.2:45823134-45823153 None:intergenic 25.0%
!!! TACTTAGATTTTTTTAGGGA+TGG - chr1.2:45823667-45823686 None:intergenic 25.0%
!!! TATTTTTCAGCGTATTGTTT+TGG - chr1.2:45823135-45823154 None:intergenic 25.0%
AAGTCTACCTTCCAATTAAT+TGG + chr1.2:45824396-45824415 MS.gene065717:intron 30.0%
ACAGATGAAAACAAAGGATT+TGG - chr1.2:45824457-45824476 None:intergenic 30.0%
AGTCTACCTTCCAATTAATT+GGG + chr1.2:45824397-45824416 MS.gene065717:intron 30.0%
ATTTCTCCCAATTAATTGGA+AGG - chr1.2:45824406-45824425 None:intergenic 30.0%
CAAAGCTATGTTCTAACAAA+AGG + chr1.2:45823264-45823283 MS.gene065717:CDS 30.0%
CTTTAATCTTCATCTTGACT+TGG + chr1.2:45823767-45823786 MS.gene065717:intron 30.0%
GTAAAGACTAATTATCGAGT+TGG - chr1.2:45823866-45823885 None:intergenic 30.0%
GTTATAATGTTACAGGTAGA+GGG + chr1.2:45824789-45824808 MS.gene065717:intron 30.0%
GTTTATAGGTTCAGAAAACT+AGG + chr1.2:45824601-45824620 MS.gene065717:intron 30.0%
TAATCTTTACAGTAGCATCT+CGG + chr1.2:45824000-45824019 MS.gene065717:intron 30.0%
TACTGTTCCAATATGAGTAA+CGG - chr1.2:45823242-45823261 None:intergenic 30.0%
TCTATAGTACCTTGTGTTAA+TGG - chr1.2:45824974-45824993 None:intergenic 30.0%
TGTTATAATGTTACAGGTAG+AGG + chr1.2:45824788-45824807 MS.gene065717:intron 30.0%
TTCGCTTTACACTAAAAACT+AGG + chr1.2:45824028-45824047 MS.gene065717:intron 30.0%
!! AAGTTCAACTTCTAGTTCAA+GGG + chr1.2:45824856-45824875 MS.gene065717:CDS 30.0%
!! AGAAGGTTGATGATATTGAT+AGG - chr1.2:45824999-45825018 None:intergenic 30.0%
!! TTGGATGGATTATAATAGCA+TGG - chr1.2:45823971-45823990 None:intergenic 30.0%
!!! ACCTAGTTTTTAGTGTAAAG+CGG - chr1.2:45823908-45823927 None:intergenic 30.0%
!!! ATGTTTTTGAGGTTGTAATG+AGG - chr1.2:45822994-45823013 None:intergenic 30.0%
!!! ATTGTTTTGGGATAACAAGA+CGG - chr1.2:45823122-45823141 None:intergenic 30.0%
!!! GAGAAATGTGGTTTTATGTT+GGG + chr1.2:45824419-45824438 MS.gene065717:intron 30.0%
!!! TAAGTTTTGAAGATGAGTCA+GGG + chr1.2:45823202-45823221 MS.gene065717:CDS 30.0%
AATAAAACCCGAAGACGAAA+AGG + chr1.2:45823047-45823066 MS.gene065717:CDS 35.0%
AATGAGGTTGTTAATGGTGT+AGG + chr1.2:45823468-45823487 MS.gene065717:CDS 35.0%
AGACATTGATCATGTTGGTA+TGG - chr1.2:45823555-45823574 None:intergenic 35.0%
AGATATATGTACCATCCTTG+AGG + chr1.2:45825068-45825087 MS.gene065717:intron 35.0%
ATCATGGTAAAGGTAGTGAT+GGG + chr1.2:45823436-45823455 MS.gene065717:CDS 35.0%
ATGAGGTTGTTAATGGTGTA+GGG + chr1.2:45823469-45823488 MS.gene065717:CDS 35.0%
ATTTGAGAATTGAACCATGG+AGG - chr1.2:45822958-45822977 None:intergenic 35.0%
CATGAAGACATTGATCATGT+TGG - chr1.2:45823560-45823579 None:intergenic 35.0%
CCAATTAATTGGGAGAAATG+TGG + chr1.2:45824407-45824426 MS.gene065717:intron 35.0%
CCACATTTCTCCCAATTAAT+TGG - chr1.2:45824410-45824429 None:intergenic 35.0%
GCTATGTTCTAACAAAAGGT+TGG + chr1.2:45823268-45823287 MS.gene065717:CDS 35.0%
GCTGAAAAATACTTTCCACT+CGG + chr1.2:45823144-45823163 MS.gene065717:CDS 35.0%
GGGTGAGAATTAGAATTAGA+AGG - chr1.2:45825016-45825035 None:intergenic 35.0%
GGTAAGAATGAGGTTGTTAA+TGG + chr1.2:45823462-45823481 MS.gene065717:CDS 35.0%
GTAACATTATAACACGTGCA+TGG - chr1.2:45824782-45824801 None:intergenic 35.0%
TAGTAAAAGTCCTTTGCACT+CGG - chr1.2:45823184-45823203 None:intergenic 35.0%
TATAGGTTCAGAAAACTAGG+CGG + chr1.2:45824604-45824623 MS.gene065717:intron 35.0%
TATTAGTCGAACCAACTTCT+TGG + chr1.2:45822914-45822933 MS.gene065717:CDS 35.0%
TCCGCTTTACACTAAAAACT+AGG + chr1.2:45823904-45823923 MS.gene065717:intron 35.0%
TGCACGTGTTATAATGTTAC+AGG + chr1.2:45824782-45824801 MS.gene065717:intron 35.0%
TGGATTTGAGAATTGAACCA+TGG - chr1.2:45822961-45822980 None:intergenic 35.0%
TTCTGATTAGGATGATGTGA+AGG - chr1.2:45824647-45824666 None:intergenic 35.0%
TTGAAGAATCATGTTCAGGT+TGG - chr1.2:45824913-45824932 None:intergenic 35.0%
! AGAGACTTTTTATCAACTGG+AGG + chr1.2:45823364-45823383 MS.gene065717:CDS 35.0%
! GTCAGAGACTTTTTATCAAC+TGG + chr1.2:45823361-45823380 MS.gene065717:CDS 35.0%
! TGTAATGAGGTGAGTTTTCA+TGG - chr1.2:45822981-45823000 None:intergenic 35.0%
!! AGACTAATTATCGAGTTGGA+CGG - chr1.2:45823862-45823881 None:intergenic 35.0%
!! ATTGATTCCTTTTCGTCTTC+GGG - chr1.2:45823057-45823076 None:intergenic 35.0%
!! CAAGTTCAACTTCTAGTTCA+AGG + chr1.2:45824855-45824874 MS.gene065717:CDS 35.0%
!! CTTCATCTTCGATGTTTTTG+AGG - chr1.2:45823005-45823024 None:intergenic 35.0%
!!! CATTGATTCCTTTTCGTCTT+CGG - chr1.2:45823058-45823077 None:intergenic 35.0%
!!! CTAAGTTTTGAAGATGAGTC+AGG + chr1.2:45823201-45823220 MS.gene065717:CDS 35.0%
!!! GGAGAAATGTGGTTTTATGT+TGG + chr1.2:45824418-45824437 MS.gene065717:intron 35.0%
!!! TTCGTCTTCGGGTTTTATTA+CGG - chr1.2:45823046-45823065 None:intergenic 35.0%
ATATGTACCATCCTTGAGGT+TGG + chr1.2:45825072-45825091 MS.gene065717:intron 40.0%
CATCATGGTAAAGGTAGTGA+TGG + chr1.2:45823435-45823454 MS.gene065717:CDS 40.0%
CATGATCAATGTCTTCATGC+AGG + chr1.2:45823561-45823580 MS.gene065717:CDS 40.0%
GAAAAATACTTTCCACTCGG+CGG + chr1.2:45823147-45823166 MS.gene065717:CDS 40.0%
TAACTTAGAACCCAACCTCA+AGG - chr1.2:45825086-45825105 None:intergenic 40.0%
TATGTACCATCCTTGAGGTT+GGG + chr1.2:45825073-45825092 MS.gene065717:intron 40.0%
TGATGGGAATGGTAAGAATG+AGG + chr1.2:45823452-45823471 MS.gene065717:CDS 40.0%
! GACTTTTTATCAACTGGAGG+AGG + chr1.2:45823367-45823386 MS.gene065717:CDS 40.0%
! GGTGTTGAAGAATCATGTTC+AGG - chr1.2:45824917-45824936 None:intergenic 40.0%
! TTGAGTTTTCCTACGTCAGA+AGG - chr1.2:45823099-45823118 None:intergenic 40.0%
! TTTTTATCAACTGGAGGAGG+AGG + chr1.2:45823370-45823389 MS.gene065717:CDS 40.0%
!! AAGGGTACTTAGGTTGTTTG+GGG + chr1.2:45824874-45824893 MS.gene065717:CDS 40.0%
!! CAAGGGTACTTAGGTTGTTT+GGG + chr1.2:45824873-45824892 MS.gene065717:CDS 40.0%
!! CTTCTAGTTCAAGGGTACTT+AGG + chr1.2:45824864-45824883 MS.gene065717:CDS 40.0%
!! TCAAGGGTACTTAGGTTGTT+TGG + chr1.2:45824872-45824891 MS.gene065717:CDS 40.0%
AAAGACAAACGACTCGATGC+TGG + chr1.2:45823303-45823322 MS.gene065717:CDS 45.0%
AATTGAACCATGGAGGTTGG+TGG - chr1.2:45822951-45822970 None:intergenic 45.0%
AGGTTGTTAATGGTGTAGGG+TGG + chr1.2:45823472-45823491 MS.gene065717:CDS 45.0%
GAACTTGAACTCTTTCCCAC+CGG - chr1.2:45824842-45824861 None:intergenic 45.0%
GAGAATTGAACCATGGAGGT+TGG - chr1.2:45822954-45822973 None:intergenic 45.0%
GGCGTTTCCGTTACTCATAT+TGG + chr1.2:45823232-45823251 MS.gene065717:CDS 45.0%
TAAAAGTCCTTTGCACTCGG+CGG - chr1.2:45823181-45823200 None:intergenic 45.0%
TCAGAACCAAAGGACGAAAC+CGG + chr1.2:45824820-45824839 MS.gene065717:CDS 45.0%
TGATGGAGAGACCAAGAAGT+TGG - chr1.2:45822928-45822947 None:intergenic 45.0%
TTAGAACCCAACCTCAAGGA+TGG - chr1.2:45825082-45825101 None:intergenic 45.0%
TTCTAATTCTCACCCTCACG+TGG + chr1.2:45825021-45825040 MS.gene065717:CDS 45.0%
! AAGATGAGTCAGGGAAGTGT+TGG + chr1.2:45823211-45823230 MS.gene065717:CDS 45.0%
! GGTAAAGGTAGTGATGGGAA+TGG + chr1.2:45823441-45823460 MS.gene065717:CDS 45.0%
! TAGGTTGTTTGGGGTCAACA+TGG + chr1.2:45824883-45824902 MS.gene065717:CDS 45.0%
! TGATGATGATGCGCAGGATA+AGG - chr1.2:45823525-45823544 None:intergenic 45.0%
!! AAGACTTGATGATGATGCGC+AGG - chr1.2:45823531-45823550 None:intergenic 45.0%
!! CCTACGTCAGAAGGTGTTAA+GGG - chr1.2:45823090-45823109 None:intergenic 45.0%
!! CGTCCTTTGGTTCTGACTTT+GGG - chr1.2:45824816-45824835 None:intergenic 45.0%
!! TCCTACGTCAGAAGGTGTTA+AGG - chr1.2:45823091-45823110 None:intergenic 45.0%
!! TCGTCCTTTGGTTCTGACTT+TGG - chr1.2:45824817-45824836 None:intergenic 45.0%
!!! GTCTTCGGGTTTTATTACGG+TGG - chr1.2:45823043-45823062 None:intergenic 45.0%
!!! TTCGGGTTTTATTACGGTGG+TGG - chr1.2:45823040-45823059 None:intergenic 45.0%
AACCAAAGGACGAAACCGGT+GGG + chr1.2:45824824-45824843 MS.gene065717:CDS 50.0%
AGGTTCAGAAAACTAGGCGG+TGG + chr1.2:45824607-45824626 MS.gene065717:intron 50.0%
AGTCGGCGTATGTTAGCAGA+GGG + chr1.2:45823406-45823425 MS.gene065717:CDS 50.0%
ATGCGCAGAATAGAACCCTC+TGG - chr1.2:45823499-45823518 None:intergenic 50.0%
CCCTTAACACCTTCTGACGT+AGG + chr1.2:45823087-45823106 MS.gene065717:CDS 50.0%
GGTCCCAAAGTCAGAACCAA+AGG + chr1.2:45824810-45824829 MS.gene065717:CDS 50.0%
AATGGTGTAGGGTGGTCCAG+AGG + chr1.2:45823480-45823499 MS.gene065717:CDS 55.0%
ATCAACTGGAGGAGGAGGAG+TGG + chr1.2:45823375-45823394 MS.gene065717:CDS 55.0%
ATGGTGTAGGGTGGTCCAGA+GGG + chr1.2:45823481-45823500 MS.gene065717:CDS 55.0%
CAGTCGGCGTATGTTAGCAG+AGG + chr1.2:45823405-45823424 MS.gene065717:CDS 55.0%
GAACCAAAGGACGAAACCGG+TGG + chr1.2:45824823-45824842 MS.gene065717:CDS 55.0%
GAGGAGTGGGGATGATCAGT+CGG + chr1.2:45823389-45823408 MS.gene065717:CDS 55.0%
GCGTATGTTAGCAGAGGGGT+GGG + chr1.2:45823411-45823430 MS.gene065717:CDS 55.0%
GGGGTGGGACATCATGGTAA+AGG + chr1.2:45823426-45823445 MS.gene065717:CDS 55.0%
GTCGGCGTATGTTAGCAGAG+GGG + chr1.2:45823407-45823426 MS.gene065717:CDS 55.0%
TCAACTGGAGGAGGAGGAGT+GGG + chr1.2:45823376-45823395 MS.gene065717:CDS 55.0%
TCCATCACCACCAACCTCCA+TGG + chr1.2:45822941-45822960 MS.gene065717:CDS 55.0%
!! ACCATGGAGGTTGGTGGTGA+TGG - chr1.2:45822945-45822964 None:intergenic 55.0%
!! GTTGGTGACGTACCACGTGA+GGG - chr1.2:45825036-45825055 None:intergenic 55.0%
!! TTCCCACCGGTTTCGTCCTT+TGG - chr1.2:45824829-45824848 None:intergenic 55.0%
AGCAGAGGGGTGGGACATCA+TGG + chr1.2:45823420-45823439 MS.gene065717:CDS 60.0%
CAACTGGAGGAGGAGGAGTG+GGG + chr1.2:45823377-45823396 MS.gene065717:CDS 60.0%
GGCGTATGTTAGCAGAGGGG+TGG + chr1.2:45823410-45823429 MS.gene065717:CDS 60.0%
!! GGTTGGTGACGTACCACGTG+AGG - chr1.2:45825037-45825056 None:intergenic 60.0%
GCAGACTCCGCCGAGTGCAA+AGG + chr1.2:45823171-45823190 MS.gene065717:CDS 65.0%
GCGGAGTCTGCTCCGCCGAG+TGG - chr1.2:45823162-45823181 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 45822910 45825137 45822910 ID=MS.gene065717
chr1.2 mRNA 45822910 45825137 45822910 ID=MS.gene065717.t1;Parent=MS.gene065717
chr1.2 exon 45822910 45823582 45822910 ID=MS.gene065717.t1.exon1;Parent=MS.gene065717.t1
chr1.2 CDS 45822910 45823582 45822910 ID=cds.MS.gene065717.t1;Parent=MS.gene065717.t1
chr1.2 exon 45824804 45825042 45824804 ID=MS.gene065717.t1.exon2;Parent=MS.gene065717.t1
chr1.2 CDS 45824804 45825042 45824804 ID=cds.MS.gene065717.t1;Parent=MS.gene065717.t1
chr1.2 exon 45825132 45825137 45825132 ID=MS.gene065717.t1.exon3;Parent=MS.gene065717.t1
chr1.2 CDS 45825132 45825137 45825132 ID=cds.MS.gene065717.t1;Parent=MS.gene065717.t1
Gene Sequence

>MS.gene065717

ATGTTATTAGTCGAACCAACTTCTTGGTCTCTCCATCACCACCAACCTCCATGGTTCAATTCTCAAATCCATGAAAACTCACCTCATTACAACCTCAAAAACATCGAAGATGAAGAATTAGACACAACCACCACCGTAATAAAACCCGAAGACGAAAAGGAATCAATGTTTGAGAAACCCTTAACACCTTCTGACGTAGGAAAACTCAACCGTCTTGTTATCCCAAAACAATACGCTGAAAAATACTTTCCACTCGGCGGAGCAGACTCCGCCGAGTGCAAAGGACTTTTACTAAGTTTTGAAGATGAGTCAGGGAAGTGTTGGCGTTTCCGTTACTCATATTGGAACAGTAGTCAAAGCTATGTTCTAACAAAAGGTTGGAGTCGTTATGTTAAAGACAAACGACTCGATGCTGGCGATGTCGTTCTGTTTGAACGACATCGTGTTGATAGTCAGAGACTTTTTATCAACTGGAGGAGGAGGAGTGGGGATGATCAGTCGGCGTATGTTAGCAGAGGGGTGGGACATCATGGTAAAGGTAGTGATGGGAATGGTAAGAATGAGGTTGTTAATGGTGTAGGGTGGTCCAGAGGGTTCTATTCTGCGCATGTTCCTTATCCTGCGCATCATCATCAAGTCTTGCCATACCAACATGATCAATGTCTTCATGCAGGTCTTAATATCTCTTCAATGCTATCTCATCTTATTTTTTACATTCACATTCTAATCAAACAAGTAAACAACTAAGTACTAACCATCCCTAAAAAAATCTAAGTACTAACTAACCATATAAATATATAATTATAATGTTCATTACCCTAATTCAACTACTTGATTGATAGTACTTAAATGGGATGCTTTAATCTTCATCTTGACTTGGTTTGAATCTAACTCTAAAAATGTAGCAGTACTGAAATTCTTGAACAAGAATTTTGTCGGCTATTATAATCCGTCCAACTCGATAATTAGTCTTTACGGTAGCATTTCAGAGATATCCGCTTTACACTAAAAACTAGGTTGATTATATAGTACAATTAAAAAAAAAAATTACCGAAAAACCATGCTATTATAATCCATCCAATTTGACAATTAATCTTTACAGTAGCATCTCGGAGATATTCGCTTTACACTAAAAACTAGGTTGATTATATATACTACAATTAAAACTATATTTTACCCTAAAACCATATTTTTCCTTTTTTCTTTCATTTTATGAGTGTGGACAATAACAATATAATTAAACTAAGCTTCCTTTTTTTTTTCTTTTTTTCTTTTTTTCTTTTCTTCTTTTATCTTTTTTGCTTAACTTTTGCTTTCAAATATGCTTGATCTTCCTGTTCTTTTTTCTATGAAAAAATTATAACAAGGAAACAACATTTTTCACACTACTTTGTTTTTCAAAAAAAAAAAAAAAAGAGAAAAAGATTTTGACCAAAAAAAAAAAAACAAAGAAGATGAAACAATTATATTCTCATGGAAGTGCGTGAAGTCTACCTTCCAATTAATTGGGAGAAATGTGGTTTTATGTTGGGATTTGTGAGAAGCCAAATCCTTTGTTTTCATCTGTTTTCTTCAAACACTGCGTGACGTATACGCTCTCATTTTCGTTCATTGAACGTGTGCAATTTTTTTCTTTCAAAACATTAATGTATGAAAAATGAAAAATATTTTTCTTTATTTCAATAATAAAAGTTTATAGGTTCAGAAAACTAGGCGGTGGTGTTTGATTTGTATCCTTCACATCATCCTAATCAGAAACGAATTTTGAATAGTCACATTTTTCTTATTTTACGGCCCCTTATTTTGTTTGTATGCAAATGCAATAGTAATAATAATATGATTATTATTATCATAGGTTAAAATTTTAATCCATGCACGTGTTATAATGTTACAGGTAGAGGGTCCCAAAGTCAGAACCAAAGGACGAAACCGGTGGGAAAGAGTTCAAGTTCAACTTCTAGTTCAAGGGTACTTAGGTTGTTTGGGGTCAACATGGAATGCCAACCTGAACATGATTCTTCAACACCTCAATGTTCTTATGATAGTAACAATAATAATATGCCATTAACACAAGGTACTATAGATACCTATCAATATCATCAACCTTCTAATTCTAATTCTCACCCTCACGTGGTACGTCACCAACCATATTACTACTAGATATATGTACCATCCTTGAGGTTGGGTTCTAAGTTATTTTTGTCTTTTTTTAATTATATTAGGTGTAA
Protein sequence

>MS.gene065717.t1

MLLVEPTSWSLHHHQPPWFNSQIHENSPHYNLKNIEDEELDTTTTVIKPEDEKESMFEKPLTPSDVGKLNRLVIPKQYAEKYFPLGGADSAECKGLLLSFEDESGKCWRFRYSYWNSSQSYVLTKGWSRYVKDKRLDAGDVVLFERHRVDSQRLFINWRRRSGDDQSAYVSRGVGHHGKGSDGNGKNEVVNGVGWSRGFYSAHVPYPAHHHQVLPYQHDQCLHAGRGSQSQNQRTKPVGKSSSSTSSSRVLRLFGVNMECQPEHDSSTPQCSYDSNNNNMPLTQGTIDTYQYHQPSNSNSHPHVV