Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065796.t1 | XP_003607414.2 | 98.8 | 163 | 2 | 0 | 1 | 163 | 393 | 555 | 3.90E-83 | 317.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065796.t1 | A0A024B7I0 | 71.9 | 167 | 42 | 2 | 1 | 163 | 423 | 588 | 5.2e-60 | 231.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065796.t1 | G7JSQ4 | 98.8 | 163 | 2 | 0 | 1 | 163 | 393 | 555 | 2.8e-83 | 317.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene065796.t1 | TF | GRAS |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065796.t1 | MTR_4g077760 | 98.773 | 163 | 2 | 0 | 1 | 163 | 393 | 555 | 1.26e-114 | 335 |
| MS.gene065796.t1 | MTR_2g082090 | 38.824 | 170 | 92 | 6 | 2 | 163 | 414 | 579 | 2.09e-22 | 93.2 |
| MS.gene065796.t1 | MTR_1g029420 | 32.941 | 170 | 102 | 5 | 2 | 163 | 427 | 592 | 5.33e-20 | 86.3 |
| MS.gene065796.t1 | MTR_7g027190 | 34.615 | 156 | 94 | 4 | 13 | 163 | 521 | 673 | 1.13e-19 | 85.1 |
| MS.gene065796.t1 | MTR_7g074650 | 35.976 | 164 | 93 | 5 | 4 | 163 | 637 | 792 | 1.45e-19 | 85.1 |
| MS.gene065796.t1 | MTR_3g089055 | 33.140 | 172 | 99 | 6 | 2 | 163 | 405 | 570 | 2.31e-19 | 84.3 |
| MS.gene065796.t1 | MTR_3g089055 | 33.140 | 172 | 99 | 6 | 2 | 163 | 400 | 565 | 2.47e-19 | 84.3 |
| MS.gene065796.t1 | MTR_5g094450 | 33.918 | 171 | 100 | 5 | 2 | 163 | 366 | 532 | 7.40e-19 | 82.8 |
| MS.gene065796.t1 | MTR_3g065980 | 36.310 | 168 | 88 | 6 | 13 | 163 | 375 | 540 | 2.25e-18 | 81.3 |
| MS.gene065796.t1 | MTR_4g076020 | 35.366 | 164 | 94 | 6 | 4 | 163 | 274 | 429 | 4.84e-18 | 80.5 |
| MS.gene065796.t1 | MTR_1g096030 | 33.728 | 169 | 92 | 4 | 15 | 163 | 291 | 459 | 1.59e-17 | 79.0 |
| MS.gene065796.t1 | MTR_3g056110 | 32.941 | 170 | 102 | 6 | 2 | 163 | 377 | 542 | 2.93e-17 | 78.2 |
| MS.gene065796.t1 | MTR_5g009080 | 30.968 | 155 | 99 | 4 | 14 | 163 | 327 | 478 | 5.71e-17 | 77.4 |
| MS.gene065796.t1 | MTR_3g022830 | 34.000 | 150 | 90 | 5 | 19 | 163 | 291 | 436 | 6.24e-17 | 77.0 |
| MS.gene065796.t1 | MTR_1g069725 | 33.775 | 151 | 92 | 4 | 17 | 162 | 315 | 462 | 1.94e-16 | 75.9 |
| MS.gene065796.t1 | MTR_5g097480 | 33.929 | 168 | 99 | 6 | 4 | 163 | 381 | 544 | 3.14e-16 | 75.1 |
| MS.gene065796.t1 | MTR_3g072710 | 35.152 | 165 | 103 | 2 | 1 | 162 | 336 | 499 | 4.23e-16 | 74.7 |
| MS.gene065796.t1 | MTR_2g026250 | 31.548 | 168 | 103 | 6 | 4 | 163 | 433 | 596 | 9.09e-16 | 73.9 |
| MS.gene065796.t1 | MTR_4g076140 | 30.345 | 145 | 93 | 4 | 24 | 163 | 328 | 469 | 1.25e-14 | 70.5 |
| MS.gene065796.t1 | MTR_4g104020 | 25.595 | 168 | 116 | 4 | 1 | 163 | 357 | 520 | 1.40e-14 | 70.5 |
| MS.gene065796.t1 | MTR_8g442410 | 28.235 | 170 | 108 | 6 | 1 | 163 | 363 | 525 | 9.62e-14 | 68.2 |
| MS.gene065796.t1 | MTR_5g058860 | 30.968 | 155 | 100 | 2 | 13 | 162 | 344 | 496 | 1.75e-11 | 61.6 |
| MS.gene065796.t1 | MTR_5g015490 | 30.405 | 148 | 92 | 4 | 24 | 163 | 346 | 490 | 2.80e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065796.t1 | AT5G66770 | 66.272 | 169 | 50 | 2 | 1 | 163 | 417 | 584 | 3.40e-71 | 224 |
| MS.gene065796.t1 | AT3G50650 | 60.116 | 173 | 58 | 3 | 1 | 163 | 371 | 542 | 4.17e-62 | 200 |
| MS.gene065796.t1 | AT3G03450 | 35.714 | 168 | 99 | 5 | 1 | 163 | 382 | 545 | 3.73e-23 | 95.1 |
| MS.gene065796.t1 | AT1G50600 | 37.059 | 170 | 95 | 5 | 2 | 163 | 436 | 601 | 1.27e-21 | 90.9 |
| MS.gene065796.t1 | AT1G50600 | 37.059 | 170 | 95 | 5 | 2 | 163 | 432 | 597 | 1.29e-21 | 90.9 |
| MS.gene065796.t1 | AT1G50600 | 37.059 | 170 | 95 | 5 | 2 | 163 | 361 | 526 | 1.35e-21 | 90.5 |
| MS.gene065796.t1 | AT5G41920 | 33.333 | 165 | 96 | 5 | 3 | 163 | 246 | 400 | 5.22e-20 | 85.9 |
| MS.gene065796.t1 | AT5G17490 | 35.484 | 155 | 92 | 4 | 14 | 163 | 365 | 516 | 2.38e-19 | 84.3 |
| MS.gene065796.t1 | AT1G55580 | 33.136 | 169 | 98 | 4 | 4 | 163 | 283 | 445 | 3.05e-19 | 84.0 |
| MS.gene065796.t1 | AT3G54220 | 36.986 | 146 | 84 | 4 | 21 | 162 | 508 | 649 | 3.73e-18 | 80.9 |
| MS.gene065796.t1 | AT5G48150 | 34.524 | 168 | 98 | 6 | 4 | 163 | 327 | 490 | 1.47e-17 | 79.0 |
| MS.gene065796.t1 | AT5G48150 | 34.524 | 168 | 98 | 6 | 4 | 163 | 327 | 490 | 1.47e-17 | 79.0 |
| MS.gene065796.t1 | AT5G48150 | 34.524 | 168 | 98 | 6 | 4 | 163 | 327 | 490 | 1.47e-17 | 79.0 |
| MS.gene065796.t1 | AT5G48150 | 34.524 | 168 | 98 | 6 | 4 | 163 | 327 | 490 | 1.47e-17 | 79.0 |
| MS.gene065796.t1 | AT1G21450 | 31.176 | 170 | 106 | 5 | 2 | 163 | 427 | 593 | 8.15e-17 | 77.0 |
| MS.gene065796.t1 | AT2G04890 | 32.143 | 168 | 102 | 6 | 4 | 163 | 250 | 413 | 1.13e-16 | 76.3 |
| MS.gene065796.t1 | AT1G66350 | 32.544 | 169 | 100 | 6 | 1 | 163 | 346 | 506 | 1.69e-16 | 76.3 |
| MS.gene065796.t1 | AT1G50420 | 32.639 | 144 | 89 | 3 | 25 | 163 | 339 | 479 | 2.84e-16 | 75.5 |
| MS.gene065796.t1 | AT1G63100 | 27.869 | 183 | 106 | 6 | 1 | 162 | 451 | 628 | 5.44e-15 | 71.6 |
| MS.gene065796.t1 | AT1G63100 | 27.869 | 183 | 106 | 6 | 1 | 162 | 476 | 653 | 6.04e-15 | 71.6 |
| MS.gene065796.t1 | AT4G17230 | 33.333 | 168 | 100 | 6 | 4 | 163 | 362 | 525 | 4.61e-14 | 68.9 |
| MS.gene065796.t1 | AT4G17230 | 33.333 | 168 | 100 | 6 | 4 | 163 | 362 | 525 | 4.61e-14 | 68.9 |
| MS.gene065796.t1 | AT1G14920 | 27.381 | 168 | 111 | 5 | 1 | 163 | 368 | 529 | 1.81e-13 | 67.4 |
| MS.gene065796.t1 | AT2G01570 | 30.588 | 170 | 103 | 7 | 1 | 163 | 420 | 581 | 4.06e-13 | 66.2 |
| MS.gene065796.t1 | AT4G08250 | 30.723 | 166 | 109 | 2 | 1 | 162 | 313 | 476 | 5.38e-13 | 65.9 |
Find 27 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTATTGACTGTTTCTTCT+TGG | 0.170351 | 4.4:+32915790 | MS.gene065796:CDS |
| GCTTCAGTTGTATAATCTTT+TGG | 0.233520 | 4.4:+32915329 | MS.gene065796:CDS |
| TCACCTAAAGTAACAATCTT+AGG | 0.256338 | 4.4:-32915405 | None:intergenic |
| AATCCTAAGATTGTTACTTT+AGG | 0.287104 | 4.4:+32915402 | MS.gene065796:CDS |
| GGATTCGCCGGAGAGGTTTC+AGG | 0.345000 | 4.4:+32915533 | MS.gene065796:CDS |
| TAGTTCTTTGTATTCTCTTG+TGG | 0.398410 | 4.4:+32915725 | MS.gene065796:CDS |
| TTGCTTGGTAGGAGAATTGA+TGG | 0.408569 | 4.4:+32915567 | MS.gene065796:CDS |
| GGCGAATCCAACGCCATGTT+CGG | 0.426377 | 4.4:-32915519 | None:intergenic |
| GTGTGATTGGTGTGAGGGAG+AGG | 0.470960 | 4.4:+32915589 | MS.gene065796:CDS |
| GATTGGTGTGAGGGAGAGGA+TGG | 0.472918 | 4.4:+32915593 | MS.gene065796:CDS |
| AGGAGAATTGATGGTGTGAT+TGG | 0.505945 | 4.4:+32915576 | MS.gene065796:CDS |
| TGAGAGTTTGGAACCGAACA+TGG | 0.520432 | 4.4:+32915506 | MS.gene065796:CDS |
| TTTGGAACCGAACATGGCGT+TGG | 0.523106 | 4.4:+32915512 | MS.gene065796:CDS |
| TGAAGCTAGTTTGACGACTC+GGG | 0.539102 | 4.4:+32915431 | MS.gene065796:CDS |
| ATGAAGCTAGTTTGACGACT+CGG | 0.543619 | 4.4:+32915430 | MS.gene065796:CDS |
| CAAGAAGAAACAGTCAATAG+AGG | 0.549819 | 4.4:-32915789 | None:intergenic |
| GAACATGGCGTTGGATTCGC+CGG | 0.557782 | 4.4:+32915521 | MS.gene065796:CDS |
| GCTAGTTTGACGACTCGGGT+TGG | 0.562442 | 4.4:+32915435 | MS.gene065796:CDS |
| GGATGGAGGATAAAGAACAG+TGG | 0.578860 | 4.4:+32915610 | MS.gene065796:CDS |
| CTCTCCACCTGAAACCTCTC+CGG | 0.581104 | 4.4:-32915540 | None:intergenic |
| GGAGGATAAAGAACAGTGGA+AGG | 0.599058 | 4.4:+32915614 | MS.gene065796:CDS |
| TGGTGTGAGGGAGAGGATGG+AGG | 0.600379 | 4.4:+32915596 | MS.gene065796:CDS |
| TGATGGTGTGATTGGTGTGA+GGG | 0.605734 | 4.4:+32915584 | MS.gene065796:CDS |
| GTCAAGCTAAGATATTGTTG+TGG | 0.613839 | 4.4:+32915691 | MS.gene065796:CDS |
| TTCGCCGGAGAGGTTTCAGG+TGG | 0.626303 | 4.4:+32915536 | MS.gene065796:CDS |
| TGGCGTTGGATTCGCCGGAG+AGG | 0.656610 | 4.4:+32915526 | MS.gene065796:CDS |
| TTGATGGTGTGATTGGTGTG+AGG | 0.659575 | 4.4:+32915583 | MS.gene065796:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATCCTAAGATTGTTACTTT+AGG | + | chr4.4:32915402-32915421 | MS.gene065796:CDS | 25.0% |
| TAGTTCTTTGTATTCTCTTG+TGG | + | chr4.4:32915725-32915744 | MS.gene065796:CDS | 30.0% | |
| TCACCTAAAGTAACAATCTT+AGG | - | chr4.4:32915408-32915427 | None:intergenic | 30.0% | |
| ! | GCTTCAGTTGTATAATCTTT+TGG | + | chr4.4:32915329-32915348 | MS.gene065796:CDS | 30.0% |
| CAAGAAGAAACAGTCAATAG+AGG | - | chr4.4:32915792-32915811 | None:intergenic | 35.0% | |
| GTCAAGCTAAGATATTGTTG+TGG | + | chr4.4:32915691-32915710 | MS.gene065796:CDS | 35.0% | |
| ! | CTCTATTGACTGTTTCTTCT+TGG | + | chr4.4:32915790-32915809 | MS.gene065796:CDS | 35.0% |
| !!! | TGCTGCTTTTTTTGAGAGTT+TGG | + | chr4.4:32915494-32915513 | MS.gene065796:CDS | 35.0% |
| TTGCTTGGTAGGAGAATTGA+TGG | + | chr4.4:32915567-32915586 | MS.gene065796:CDS | 40.0% | |
| ! | ATGAAGCTAGTTTGACGACT+CGG | + | chr4.4:32915430-32915449 | MS.gene065796:CDS | 40.0% |
| !! | AAGGTTTTGATGGAAAGCTG+TGG | + | chr4.4:32915633-32915652 | MS.gene065796:CDS | 40.0% |
| !! | AGAACAGTGGAAGGTTTTGA+TGG | + | chr4.4:32915623-32915642 | MS.gene065796:CDS | 40.0% |
| !! | AGGAGAATTGATGGTGTGAT+TGG | + | chr4.4:32915576-32915595 | MS.gene065796:CDS | 40.0% |
| !!! | AAGCTGTGGTTTTGAATCAG+TGG | + | chr4.4:32915647-32915666 | MS.gene065796:CDS | 40.0% |
| !!! | AGCTGTGGTTTTGAATCAGT+GGG | + | chr4.4:32915648-32915667 | MS.gene065796:CDS | 40.0% |
| !!! | CAAACGAAGCGCTTTTTCAA+CGG | - | chr4.4:32915370-32915389 | None:intergenic | 40.0% |
| !!! | TCAACCTGCTTTTTTGTCTC+TGG | + | chr4.4:32915752-32915771 | MS.gene065796:CDS | 40.0% |
| CAAGCCAGAGACAAAAAAGC+AGG | - | chr4.4:32915759-32915778 | None:intergenic | 45.0% | |
| GGAGGATAAAGAACAGTGGA+AGG | + | chr4.4:32915614-32915633 | MS.gene065796:CDS | 45.0% | |
| GGATGGAGGATAAAGAACAG+TGG | + | chr4.4:32915610-32915629 | MS.gene065796:CDS | 45.0% | |
| ! | TGAAGCTAGTTTGACGACTC+GGG | + | chr4.4:32915431-32915450 | MS.gene065796:CDS | 45.0% |
| ! | TGAGAGTTTGGAACCGAACA+TGG | + | chr4.4:32915506-32915525 | MS.gene065796:CDS | 45.0% |
| ! | TGATGGTGTGATTGGTGTGA+GGG | + | chr4.4:32915584-32915603 | MS.gene065796:CDS | 45.0% |
| ! | TGGAGAGTCTTTTGCTTGGT+AGG | + | chr4.4:32915556-32915575 | MS.gene065796:CDS | 45.0% |
| !! | CGTTGAAAAAGCGCTTCGTT+TGG | + | chr4.4:32915368-32915387 | MS.gene065796:CDS | 45.0% |
| !! | TTGATGGTGTGATTGGTGTG+AGG | + | chr4.4:32915583-32915602 | MS.gene065796:CDS | 45.0% |
| !!! | CTGCTTTTTTGTCTCTGGCT+TGG | + | chr4.4:32915757-32915776 | MS.gene065796:CDS | 45.0% |
| ! | CAGGTGGAGAGTCTTTTGCT+TGG | + | chr4.4:32915552-32915571 | MS.gene065796:CDS | 50.0% |
| ! | TTTGGAACCGAACATGGCGT+TGG | + | chr4.4:32915512-32915531 | MS.gene065796:CDS | 50.0% |
| CTCTCCACCTGAAACCTCTC+CGG | - | chr4.4:32915543-32915562 | None:intergenic | 55.0% | |
| GATTGGTGTGAGGGAGAGGA+TGG | + | chr4.4:32915593-32915612 | MS.gene065796:CDS | 55.0% | |
| GGCGAATCCAACGCCATGTT+CGG | - | chr4.4:32915522-32915541 | None:intergenic | 55.0% | |
| GTGTGATTGGTGTGAGGGAG+AGG | + | chr4.4:32915589-32915608 | MS.gene065796:CDS | 55.0% | |
| ! | GAACATGGCGTTGGATTCGC+CGG | + | chr4.4:32915521-32915540 | MS.gene065796:CDS | 55.0% |
| ! | GCTAGTTTGACGACTCGGGT+TGG | + | chr4.4:32915435-32915454 | MS.gene065796:CDS | 55.0% |
| GGATTCGCCGGAGAGGTTTC+AGG | + | chr4.4:32915533-32915552 | MS.gene065796:CDS | 60.0% | |
| TGGTGTGAGGGAGAGGATGG+AGG | + | chr4.4:32915596-32915615 | MS.gene065796:CDS | 60.0% | |
| TTCGCCGGAGAGGTTTCAGG+TGG | + | chr4.4:32915536-32915555 | MS.gene065796:CDS | 60.0% | |
| ! | TGGCGTTGGATTCGCCGGAG+AGG | + | chr4.4:32915526-32915545 | MS.gene065796:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 32915327 | 32915818 | 32915327 | ID=MS.gene065796 |
| chr4.4 | mRNA | 32915327 | 32915818 | 32915327 | ID=MS.gene065796.t1;Parent=MS.gene065796 |
| chr4.4 | exon | 32915327 | 32915818 | 32915327 | ID=MS.gene065796.t1.exon1;Parent=MS.gene065796.t1 |
| chr4.4 | CDS | 32915327 | 32915818 | 32915327 | ID=cds.MS.gene065796.t1;Parent=MS.gene065796.t1 |
| Gene Sequence |
| Protein sequence |
