AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065797

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065797.t1 MTR_4g077760 97.706 436 10 0 1 436 120 555 0.0 866
MS.gene065797.t1 MTR_2g082090 38.068 352 193 12 100 436 238 579 1.56e-54 191
MS.gene065797.t1 MTR_3g056110 35.876 354 210 10 94 436 195 542 2.22e-54 189
MS.gene065797.t1 MTR_5g094450 35.196 358 211 9 94 436 181 532 4.22e-54 189
MS.gene065797.t1 MTR_3g065980 35.401 387 214 13 71 436 169 540 5.22e-54 189
MS.gene065797.t1 MTR_7g074650 34.332 367 219 10 77 436 441 792 2.46e-53 191
MS.gene065797.t1 MTR_7g027190 33.065 372 210 9 90 436 316 673 2.71e-52 186
MS.gene065797.t1 MTR_3g089055 31.383 376 235 8 75 436 199 565 7.79e-51 181
MS.gene065797.t1 MTR_3g089055 31.383 376 235 8 75 436 204 570 1.04e-50 180
MS.gene065797.t1 MTR_1g029420 31.452 372 240 7 75 436 226 592 2.14e-50 180
MS.gene065797.t1 MTR_5g097480 33.684 380 237 9 67 436 170 544 2.34e-50 179
MS.gene065797.t1 MTR_1g069725 33.871 372 224 10 71 435 106 462 3.60e-50 177
MS.gene065797.t1 MTR_4g076020 32.778 360 200 13 88 436 101 429 6.40e-46 164
MS.gene065797.t1 MTR_3g072710 31.536 371 232 7 84 435 132 499 2.87e-45 164
MS.gene065797.t1 MTR_4g102790 30.287 383 239 12 71 435 289 661 5.10e-45 166
MS.gene065797.t1 MTR_4g104020 27.792 385 249 7 71 436 146 520 3.89e-44 161
MS.gene065797.t1 MTR_4g133660 32.286 350 220 9 97 435 199 542 5.81e-44 161
MS.gene065797.t1 MTR_1g096030 32.152 395 229 12 70 436 76 459 1.24e-43 159
MS.gene065797.t1 MTR_2g026250 31.161 353 222 10 97 436 252 596 8.65e-42 156
MS.gene065797.t1 MTR_4g097080 28.974 390 251 10 62 436 125 503 2.16e-40 151
MS.gene065797.t1 MTR_3g022830 28.009 432 239 13 65 436 17 436 1.46e-39 147
MS.gene065797.t1 MTR_5g058860 31.232 349 223 6 102 435 150 496 2.63e-39 148
MS.gene065797.t1 MTR_2g097310 28.684 380 244 10 72 435 269 637 1.17e-37 145
MS.gene065797.t1 MTR_4g064200 27.676 383 255 9 66 435 276 649 1.51e-37 145
MS.gene065797.t1 MTR_2g097410 26.772 381 254 10 72 435 368 740 3.72e-37 144
MS.gene065797.t1 MTR_4g064160 29.024 379 247 9 71 435 313 683 7.13e-37 143
MS.gene065797.t1 MTR_5g009080 25.688 436 244 9 71 436 53 478 1.00e-36 140
MS.gene065797.t1 MTR_8g442410 28.571 385 245 10 71 436 152 525 2.38e-36 140
MS.gene065797.t1 MTR_2g097350 28.421 380 245 10 72 435 271 639 3.53e-36 140
MS.gene065797.t1 MTR_4g064150 27.473 364 238 9 90 435 377 732 5.08e-36 141
MS.gene065797.t1 MTR_5g015490 29.517 393 241 12 67 436 111 490 7.75e-36 138
MS.gene065797.t1 MTR_2g097390 28.684 380 244 10 72 435 272 640 1.09e-35 139
MS.gene065797.t1 MTR_4g076140 26.545 437 238 12 71 436 45 469 1.12e-35 137
MS.gene065797.t1 MTR_8g093070 32.402 358 208 15 91 435 159 495 6.76e-35 135
MS.gene065797.t1 MTR_2g034250 27.062 388 252 11 71 436 207 585 1.23e-34 136
MS.gene065797.t1 MTR_4g064120 25.441 397 278 8 43 425 223 615 1.64e-34 136
MS.gene065797.t1 MTR_2g034280 26.702 382 258 9 71 436 200 575 1.74e-34 135
MS.gene065797.t1 MTR_2g097463 26.455 378 255 8 71 435 287 654 1.89e-34 136
MS.gene065797.t1 MTR_2g097467 25.464 377 260 7 71 435 286 653 2.83e-34 135
MS.gene065797.t1 MTR_2g034260 26.562 384 258 10 71 436 207 584 4.43e-34 134
MS.gene065797.t1 MTR_4g064180 27.429 350 236 8 90 425 270 615 5.00e-34 134
MS.gene065797.t1 MTR_4g026485 29.639 415 240 16 44 436 240 624 6.88e-34 134
MS.gene065797.t1 MTR_7g062120 26.703 367 246 9 89 436 378 740 3.10e-33 133
MS.gene065797.t1 MTR_2g097473 25.464 377 260 7 71 435 286 653 6.93e-33 131
MS.gene065797.t1 MTR_7g109580 29.076 368 244 10 79 436 194 554 1.85e-32 129
MS.gene065797.t1 MTR_2g097380 27.019 359 211 10 90 435 240 560 9.38e-32 127
MS.gene065797.t1 MTR_0092s0100 30.933 375 235 12 71 436 369 728 2.67e-31 127
MS.gene065797.t1 MTR_0092s0100 30.933 375 235 12 71 436 372 731 2.79e-31 127
MS.gene065797.t1 MTR_2g099110 27.735 393 241 11 73 436 73 451 4.93e-29 118
MS.gene065797.t1 MTR_2g089100 27.000 400 247 14 73 435 51 442 2.50e-26 110
MS.gene065797.t1 MTR_5g015950 24.429 438 263 13 61 435 13 445 4.45e-24 104
MS.gene065797.t1 MTR_3g053270 24.429 438 263 13 61 435 13 445 4.45e-24 104
MS.gene065797.t1 MTR_7g057230 29.594 419 251 17 39 435 260 656 1.04e-23 104
MS.gene065797.t1 MTR_4g095500 24.236 458 266 15 38 435 31 467 2.42e-20 93.6
MS.gene065797.t1 MTR_1g086970 21.271 362 251 10 96 436 130 478 1.46e-19 91.3
MS.gene065797.t1 MTR_8g020840 26.016 369 248 10 88 436 185 548 3.51e-19 90.5
MS.gene065797.t1 MTR_8g077940 25.770 357 217 13 102 436 211 541 7.91e-16 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065797.t1 AT5G66770 56.000 475 163 8 1 436 117 584 9.29e-166 479
MS.gene065797.t1 AT3G50650 59.947 377 131 5 70 436 176 542 1.43e-150 439
MS.gene065797.t1 AT5G41920 32.843 408 233 12 47 436 16 400 2.69e-56 191
MS.gene065797.t1 AT5G48150 34.646 381 233 10 67 436 115 490 3.96e-55 190
MS.gene065797.t1 AT5G48150 34.646 381 233 10 67 436 115 490 3.96e-55 190
MS.gene065797.t1 AT5G48150 34.646 381 233 10 67 436 115 490 3.96e-55 190
MS.gene065797.t1 AT5G48150 34.646 381 233 10 67 436 115 490 3.96e-55 190
MS.gene065797.t1 AT1G50600 35.000 380 232 9 67 436 152 526 1.73e-54 189
MS.gene065797.t1 AT3G03450 34.127 378 225 13 71 436 180 545 3.93e-54 189
MS.gene065797.t1 AT1G50600 35.000 380 232 9 67 436 227 601 6.75e-54 189
MS.gene065797.t1 AT1G50600 35.000 380 232 9 67 436 223 597 7.31e-54 189
MS.gene065797.t1 AT3G54220 33.875 369 219 10 77 435 296 649 2.02e-53 189
MS.gene065797.t1 AT2G04890 34.085 355 210 12 93 436 72 413 1.11e-50 177
MS.gene065797.t1 AT5G17490 32.061 393 244 12 54 436 137 516 3.59e-47 170
MS.gene065797.t1 AT1G66350 30.909 440 252 18 23 436 93 506 5.14e-46 166
MS.gene065797.t1 AT1G21450 28.244 393 267 8 54 436 206 593 7.62e-45 164
MS.gene065797.t1 AT1G63100 31.776 321 184 8 137 435 321 628 1.51e-44 164
MS.gene065797.t1 AT1G63100 31.776 321 184 8 137 435 346 653 2.74e-44 164
MS.gene065797.t1 AT2G01570 33.141 347 206 14 100 436 251 581 1.99e-43 160
MS.gene065797.t1 AT1G55580 31.842 380 204 9 104 436 74 445 2.48e-43 158
MS.gene065797.t1 AT4G17230 31.649 376 242 8 71 436 155 525 4.35e-43 159
MS.gene065797.t1 AT4G17230 31.649 376 242 8 71 436 155 525 4.35e-43 159
MS.gene065797.t1 AT1G14920 31.805 349 208 13 100 436 199 529 1.24e-42 157
MS.gene065797.t1 AT3G46600 27.763 371 255 7 75 436 5 371 4.43e-41 150
MS.gene065797.t1 AT4G08250 29.429 350 237 5 94 435 129 476 4.10e-40 150
MS.gene065797.t1 AT3G46600 27.763 371 255 7 75 436 181 547 7.45e-40 150
MS.gene065797.t1 AT3G46600 27.763 371 255 7 75 436 213 579 1.14e-39 150
MS.gene065797.t1 AT1G07520 28.729 362 239 7 90 435 170 528 4.15e-39 148
MS.gene065797.t1 AT1G07520 28.729 362 239 7 90 435 170 528 4.15e-39 148
MS.gene065797.t1 AT1G07520 28.729 362 239 7 90 435 334 692 1.07e-38 148
MS.gene065797.t1 AT1G07520 28.729 362 239 7 90 435 335 693 1.20e-38 148
MS.gene065797.t1 AT5G59450 26.847 406 273 9 48 436 200 598 2.67e-38 147
MS.gene065797.t1 AT4G37650 29.365 378 237 11 83 436 158 529 3.40e-38 145
MS.gene065797.t1 AT1G07530 26.633 398 270 9 52 435 375 764 5.99e-37 144
MS.gene065797.t1 AT2G29065 27.763 389 239 10 75 435 253 627 1.82e-36 142
MS.gene065797.t1 AT2G29060 25.178 421 296 10 29 435 239 654 5.40e-36 140
MS.gene065797.t1 AT2G29060 25.178 421 296 10 29 435 276 691 6.12e-36 140
MS.gene065797.t1 AT2G37650 25.217 345 236 7 105 436 378 713 5.17e-29 120
MS.gene065797.t1 AT2G37650 25.217 345 236 7 105 436 366 701 7.20e-29 120
MS.gene065797.t1 AT4G00150 28.338 367 224 13 86 436 211 554 1.08e-26 112
MS.gene065797.t1 AT5G52510 26.615 387 248 12 73 436 267 640 2.07e-26 112
MS.gene065797.t1 AT1G50420 31.878 229 135 7 85 305 71 286 6.94e-25 107
MS.gene065797.t1 AT1G50420 32.414 145 90 3 297 436 338 479 4.76e-15 77.4
MS.gene065797.t1 AT2G45160 28.070 399 227 16 71 436 268 639 1.40e-24 107
MS.gene065797.t1 AT3G60630 28.488 344 220 9 107 436 291 622 1.14e-23 104
MS.gene065797.t1 AT3G49950 28.011 357 223 12 104 435 60 407 1.16e-23 102
MS.gene065797.t1 AT3G13840 25.459 381 250 9 73 436 147 510 2.20e-20 94.0
MS.gene065797.t1 AT4G36710 23.947 380 259 12 71 436 122 485 8.31e-17 82.8

Find 87 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAATAGTGTAAGAATTTC+TGG 0.119456 4.4:-32925586 MS.gene065797:CDS
AATATTCATATAGTTGATTT+TGG 0.139541 4.4:-32925673 MS.gene065797:CDS
CTCTATTGACTGTTTCTTCT+TGG 0.170351 4.4:-32924916 MS.gene065797:CDS
CCAGAAATTCTTACACTATT+AGG 0.205458 4.4:+32925586 None:intergenic
ATATGGGCATGCATCGTTTA+AGG 0.214693 4.4:+32925756 None:intergenic
GCTTCAGTTGTATAATCTTT+TGG 0.233520 4.4:-32925377 MS.gene065797:CDS
TAAAAGGAAACTCTTTGATT+AGG 0.234171 4.4:+32925880 None:intergenic
GGTATACCTGCTATGGCTTT+AGG 0.252034 4.4:-32925565 MS.gene065797:CDS
TCACCTAAAGTAACAATCTT+AGG 0.256338 4.4:+32925301 None:intergenic
AAATGTGCGAACTTTGAATA+TGG 0.257358 4.4:+32925739 None:intergenic
AAAAGGAAACTCTTTGATTA+GGG 0.285344 4.4:+32925881 None:intergenic
AATCCTAAGATTGTTACTTT+AGG 0.287104 4.4:-32925304 MS.gene065797:CDS
TGTGGAGTGGGTGGTTGGAT+TGG 0.309124 4.4:+32926072 None:intergenic
AGGGTTTCAATTGCTTGATT+TGG 0.313945 4.4:+32925940 None:intergenic
GTGGAGTGGGTGGTTGGATT+GGG 0.321937 4.4:+32926073 None:intergenic
TTCGATGAGTGAAGCAATTT+CGG 0.323142 4.4:+32925972 None:intergenic
CAAATTCTTCTTCAACTACT+TGG 0.323189 4.4:-32925804 MS.gene065797:CDS
TAGGGCTTGTGAAAAGTAAA+AGG 0.330479 4.4:+32925864 None:intergenic
GGATTCGCCGGAGAGGTTTC+AGG 0.345000 4.4:-32925173 MS.gene065797:CDS
GCTATCGGCGGCGGACATTA+AGG 0.345403 4.4:+32926191 None:intergenic
TCGATTTACTGAGATGGGTT+AGG 0.351223 4.4:+32925920 None:intergenic
GCATTTGCAACTCGTTCTTC+TGG 0.357528 4.4:-32925613 MS.gene065797:CDS
TTAACAGCTAAAGCCTCATT+TGG 0.358736 4.4:+32925406 None:intergenic
ATCAACTATATGAATATTGT+TGG 0.382716 4.4:+32925678 None:intergenic
TAGTTCTTTGTATTCTCTTG+TGG 0.398410 4.4:-32924981 MS.gene065797:CDS
CGATTTACTGAGATGGGTTA+GGG 0.403048 4.4:+32925921 None:intergenic
TGAAAACGACGATGTCGTTT+CGG 0.406416 4.4:+32926026 None:intergenic
TTGCTTGGTAGGAGAATTGA+TGG 0.408569 4.4:-32925139 MS.gene065797:CDS
TGAGTGAAGCAATTTCGGTT+AGG 0.410733 4.4:+32925977 None:intergenic
GAGTGAAGCAATTTCGGTTA+GGG 0.413751 4.4:+32925978 None:intergenic
GAATTGTTCAAGGGATTCAA+TGG 0.414040 4.4:-32925651 MS.gene065797:CDS
GACCCGACTCTCACCACAAC+CGG 0.424081 4.4:-32926130 MS.gene065797:CDS
GGCGAATCCAACGCCATGTT+CGG 0.426377 4.4:+32925187 None:intergenic
ATTTGAAGCGGTACGGTGGC+GGG 0.431027 4.4:+32926096 None:intergenic
GATTCATCAAGAAGCTCAAT+AGG 0.436690 4.4:+32925445 None:intergenic
GATTTGAAGCGGTACGGTGG+CGG 0.443082 4.4:+32926095 None:intergenic
TGAAATCGATTTACTGAGAT+GGG 0.451988 4.4:+32925915 None:intergenic
ATCCGGTTGTGGTGAGAGTC+GGG 0.456439 4.4:+32926128 None:intergenic
GTAGCTGAAATCGATGAAAC+TGG 0.464413 4.4:+32925535 None:intergenic
TGCGTTGTGGAGTGGGTGGT+TGG 0.467268 4.4:+32926067 None:intergenic
GTGTGATTGGTGTGAGGGAG+AGG 0.470960 4.4:-32925117 MS.gene065797:CDS
AATTTCTGGTATACCTGCTA+TGG 0.471549 4.4:-32925572 MS.gene065797:CDS
GATTGGTGTGAGGGAGAGGA+TGG 0.472918 4.4:-32925113 MS.gene065797:CDS
AATGTGCGAACTTTGAATAT+GGG 0.473696 4.4:+32925740 None:intergenic
GTTTCATCGATTTCAGCTAC+TGG 0.480562 4.4:-32925532 MS.gene065797:CDS
GTGAAATCGATTTACTGAGA+TGG 0.489246 4.4:+32925914 None:intergenic
CGGGTCGGGCATGATGAAAA+CGG 0.497777 4.4:+32926147 None:intergenic
ATCGGAGTGGCTATCGGCGG+CGG 0.500643 4.4:+32926182 None:intergenic
AGGAGAATTGATGGTGTGAT+TGG 0.505945 4.4:-32925130 MS.gene065797:CDS
AAACGGGTTGAGATGGAAAT+CGG 0.511523 4.4:+32926164 None:intergenic
GGGTCGGGCATGATGAAAAC+GGG 0.514429 4.4:+32926148 None:intergenic
TGAGAGTTTGGAACCGAACA+TGG 0.520432 4.4:-32925200 MS.gene065797:CDS
AAATTCTTCTTCAACTACTT+GGG 0.520685 4.4:-32925803 MS.gene065797:CDS
TTTGGAACCGAACATGGCGT+TGG 0.523106 4.4:-32925194 MS.gene065797:CDS
GATTGGGATTTGAAGCGGTA+CGG 0.528703 4.4:+32926089 None:intergenic
ATGGAAATCGGAGTGGCTAT+CGG 0.534057 4.4:+32926176 None:intergenic
AATTGTTCAAGGGATTCAAT+GGG 0.536168 4.4:-32925650 MS.gene065797:CDS
GTTGTGGTGAGAGTCGGGTC+GGG 0.536815 4.4:+32926133 None:intergenic
GTGGCGGGTAAGAGAAGATC+CGG 0.537694 4.4:+32926111 None:intergenic
AGCTCAATAGGCGTAAGAAT+CGG 0.539095 4.4:+32925457 None:intergenic
TGAAGCTAGTTTGACGACTC+GGG 0.539102 4.4:-32925275 MS.gene065797:CDS
ATGAAGCTAGTTTGACGACT+CGG 0.543619 4.4:-32925276 MS.gene065797:CDS
GATCCGGTTGTGGTGAGAGT+CGG 0.547170 4.4:+32926127 None:intergenic
CAAGAAGAAACAGTCAATAG+AGG 0.549819 4.4:+32924917 None:intergenic
AGTAAATCGATTTCACAAAA+CGG 0.553570 4.4:-32925907 MS.gene065797:CDS
GAACATGGCGTTGGATTCGC+CGG 0.557782 4.4:-32925185 MS.gene065797:CDS
GCTAGTTTGACGACTCGGGT+TGG 0.562442 4.4:-32925271 MS.gene065797:CDS
GAAGTTCCTAAAGCCATAGC+AGG 0.575286 4.4:+32925559 None:intergenic
GGATGGAGGATAAAGAACAG+TGG 0.578860 4.4:-32925096 MS.gene065797:CDS
CTCTCCACCTGAAACCTCTC+CGG 0.581104 4.4:+32925166 None:intergenic
GGTTGTGGTGAGAGTCGGGT+CGG 0.584555 4.4:+32926132 None:intergenic
ATGATGAAAACGGGTTGAGA+TGG 0.594967 4.4:+32926157 None:intergenic
GGAGGATAAAGAACAGTGGA+AGG 0.599058 4.4:-32925092 MS.gene065797:CDS
GGTTGGATTGGGATTTGAAG+CGG 0.600133 4.4:+32926084 None:intergenic
TGGTGTGAGGGAGAGGATGG+AGG 0.600379 4.4:-32925110 MS.gene065797:CDS
GCAATTCTTGAAGCAACAGA+AGG 0.601796 4.4:-32925703 MS.gene065797:CDS
TGATGGTGTGATTGGTGTGA+GGG 0.605734 4.4:-32925122 MS.gene065797:CDS
GTCAAGCTAAGATATTGTTG+TGG 0.613839 4.4:-32925015 MS.gene065797:CDS
TGGGATTTGAAGCGGTACGG+TGG 0.615546 4.4:+32926092 None:intergenic
TTCGCCGGAGAGGTTTCAGG+TGG 0.626303 4.4:-32925170 MS.gene065797:CDS
GGTAAGAGAAGATCCGGTTG+TGG 0.627421 4.4:+32926117 None:intergenic
TGGCGTTGGATTCGCCGGAG+AGG 0.656610 4.4:-32925180 MS.gene065797:CDS
TTGATGGTGTGATTGGTGTG+AGG 0.659575 4.4:-32925123 MS.gene065797:CDS
GAAATCGGAGTGGCTATCGG+CGG 0.678873 4.4:+32926179 None:intergenic
AGAAGCTATTGAAGATTGTG+AGG 0.683841 4.4:+32925828 None:intergenic
AAACGACGATGTCGTTTCGG+TGG 0.687537 4.4:+32926029 None:intergenic
GGTTGAGATGGAAATCGGAG+TGG 0.716922 4.4:+32926169 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTCATATAGTTGATTT+TGG - chr4.4:32925435-32925454 MS.gene065797:CDS 15.0%
!! ATCAACTATATGAATATTGT+TGG + chr4.4:32925433-32925452 None:intergenic 20.0%
! AAAAGGAAACTCTTTGATTA+GGG + chr4.4:32925230-32925249 None:intergenic 25.0%
! AAATTCTTCTTCAACTACTT+GGG - chr4.4:32925305-32925324 MS.gene065797:CDS 25.0%
! TAAAAGGAAACTCTTTGATT+AGG + chr4.4:32925231-32925250 None:intergenic 25.0%
!! AGTAAATCGATTTCACAAAA+CGG - chr4.4:32925201-32925220 MS.gene065797:CDS 25.0%
!!! AATCCTAAGATTGTTACTTT+AGG - chr4.4:32925804-32925823 MS.gene065797:CDS 25.0%
!!! AATTGCTTGATTTGGTTTTT+GGG + chr4.4:32925163-32925182 None:intergenic 25.0%
!!! TTGATTTTGGAATTGTTCAA+GGG - chr4.4:32925448-32925467 MS.gene065797:CDS 25.0%
AAATGTGCGAACTTTGAATA+TGG + chr4.4:32925372-32925391 None:intergenic 30.0%
AATGTGCGAACTTTGAATAT+GGG + chr4.4:32925371-32925390 None:intergenic 30.0%
AATTGTTCAAGGGATTCAAT+GGG - chr4.4:32925458-32925477 MS.gene065797:CDS 30.0%
CAAATTCTTCTTCAACTACT+TGG - chr4.4:32925304-32925323 MS.gene065797:CDS 30.0%
CCAGAAATTCTTACACTATT+AGG + chr4.4:32925525-32925544 None:intergenic 30.0%
CCTAATAGTGTAAGAATTTC+TGG - chr4.4:32925522-32925541 MS.gene065797:CDS 30.0%
TAGTTCTTTGTATTCTCTTG+TGG - chr4.4:32926127-32926146 MS.gene065797:CDS 30.0%
TCACCTAAAGTAACAATCTT+AGG + chr4.4:32925810-32925829 None:intergenic 30.0%
! GCTTCAGTTGTATAATCTTT+TGG - chr4.4:32925731-32925750 MS.gene065797:CDS 30.0%
! TGAAATCGATTTACTGAGAT+GGG + chr4.4:32925196-32925215 None:intergenic 30.0%
!!! CAATTGCTTGATTTGGTTTT+TGG + chr4.4:32925164-32925183 None:intergenic 30.0%
!!! GTTGATTTTGGAATTGTTCA+AGG - chr4.4:32925447-32925466 MS.gene065797:CDS 30.0%
AGAAGCTATTGAAGATTGTG+AGG + chr4.4:32925283-32925302 None:intergenic 35.0%
AGGGTTTCAATTGCTTGATT+TGG + chr4.4:32925171-32925190 None:intergenic 35.0%
CAAGAAGAAACAGTCAATAG+AGG + chr4.4:32926194-32926213 None:intergenic 35.0%
GAATTGTTCAAGGGATTCAA+TGG - chr4.4:32925457-32925476 MS.gene065797:CDS 35.0%
GATTCATCAAGAAGCTCAAT+AGG + chr4.4:32925666-32925685 None:intergenic 35.0%
GTCAAGCTAAGATATTGTTG+TGG - chr4.4:32926093-32926112 MS.gene065797:CDS 35.0%
TAGGGCTTGTGAAAAGTAAA+AGG + chr4.4:32925247-32925266 None:intergenic 35.0%
TTAACAGCTAAAGCCTCATT+TGG + chr4.4:32925705-32925724 None:intergenic 35.0%
TTCGATGAGTGAAGCAATTT+CGG + chr4.4:32925139-32925158 None:intergenic 35.0%
! AGATTGTGAGGTGATTTTGT+TGG + chr4.4:32925271-32925290 None:intergenic 35.0%
! CTCTATTGACTGTTTCTTCT+TGG - chr4.4:32926192-32926211 MS.gene065797:CDS 35.0%
! CTTTTGTATTCAGCCAAATG+AGG - chr4.4:32925689-32925708 MS.gene065797:CDS 35.0%
! GTGAAATCGATTTACTGAGA+TGG + chr4.4:32925197-32925216 None:intergenic 35.0%
!! AATTTCTGGTATACCTGCTA+TGG - chr4.4:32925536-32925555 MS.gene065797:CDS 35.0%
!! TGAGGTGATTTTGTTGGTTA+GGG + chr4.4:32925265-32925284 None:intergenic 35.0%
!!! TGCTGCTTTTTTTGAGAGTT+TGG - chr4.4:32925896-32925915 MS.gene065797:CDS 35.0%
AGCTCAATAGGCGTAAGAAT+CGG + chr4.4:32925654-32925673 None:intergenic 40.0%
ATATGGGCATGCATCGTTTA+AGG + chr4.4:32925355-32925374 None:intergenic 40.0%
GAGTGAAGCAATTTCGGTTA+GGG + chr4.4:32925133-32925152 None:intergenic 40.0%
GCAATTCTTGAAGCAACAGA+AGG - chr4.4:32925405-32925424 MS.gene065797:CDS 40.0%
GTAGCTGAAATCGATGAAAC+TGG + chr4.4:32925576-32925595 None:intergenic 40.0%
TGAGTGAAGCAATTTCGGTT+AGG + chr4.4:32925134-32925153 None:intergenic 40.0%
TTGCTTGGTAGGAGAATTGA+TGG - chr4.4:32925969-32925988 MS.gene065797:CDS 40.0%
! ATGAAGCTAGTTTGACGACT+CGG - chr4.4:32925832-32925851 MS.gene065797:CDS 40.0%
! TCGATTTACTGAGATGGGTT+AGG + chr4.4:32925191-32925210 None:intergenic 40.0%
! TGAAAACGACGATGTCGTTT+CGG + chr4.4:32925085-32925104 None:intergenic 40.0%
!! AAACGGGTTGAGATGGAAAT+CGG + chr4.4:32924947-32924966 None:intergenic 40.0%
!! AAGGTTTTGATGGAAAGCTG+TGG - chr4.4:32926035-32926054 MS.gene065797:CDS 40.0%
!! AGAACAGTGGAAGGTTTTGA+TGG - chr4.4:32926025-32926044 MS.gene065797:CDS 40.0%
!! AGGAGAATTGATGGTGTGAT+TGG - chr4.4:32925978-32925997 MS.gene065797:CDS 40.0%
!! ATGATGAAAACGGGTTGAGA+TGG + chr4.4:32924954-32924973 None:intergenic 40.0%
!! CGATTTACTGAGATGGGTTA+GGG + chr4.4:32925190-32925209 None:intergenic 40.0%
!! GTGAGGTGATTTTGTTGGTT+AGG + chr4.4:32925266-32925285 None:intergenic 40.0%
!! GTTTCATCGATTTCAGCTAC+TGG - chr4.4:32925576-32925595 MS.gene065797:CDS 40.0%
!!! AAGCTGTGGTTTTGAATCAG+TGG - chr4.4:32926049-32926068 MS.gene065797:CDS 40.0%
!!! AGCTGTGGTTTTGAATCAGT+GGG - chr4.4:32926050-32926069 MS.gene065797:CDS 40.0%
!!! CAAACGAAGCGCTTTTTCAA+CGG + chr4.4:32925772-32925791 None:intergenic 40.0%
!!! CGGTTAGGGTTTTAAGAAGT+GGG + chr4.4:32925119-32925138 None:intergenic 40.0%
!!! GGTTTTAAGAAGTGGGTTTG+TGG + chr4.4:32925112-32925131 None:intergenic 40.0%
!!! TCAACCTGCTTTTTTGTCTC+TGG - chr4.4:32926154-32926173 MS.gene065797:CDS 40.0%
!!! TCGGTTAGGGTTTTAAGAAG+TGG + chr4.4:32925120-32925139 None:intergenic 40.0%
!!! TTAGTGTCGTTTTTGCGTTG+TGG + chr4.4:32925057-32925076 None:intergenic 40.0%
ATGGAAATCGGAGTGGCTAT+CGG + chr4.4:32924935-32924954 None:intergenic 45.0%
CAAGCCAGAGACAAAAAAGC+AGG + chr4.4:32926161-32926180 None:intergenic 45.0%
GAAGTTCCTAAAGCCATAGC+AGG + chr4.4:32925552-32925571 None:intergenic 45.0%
GCATTTGCAACTCGTTCTTC+TGG - chr4.4:32925495-32925514 MS.gene065797:CDS 45.0%
GGAGGATAAAGAACAGTGGA+AGG - chr4.4:32926016-32926035 MS.gene065797:CDS 45.0%
GGATGGAGGATAAAGAACAG+TGG - chr4.4:32926012-32926031 MS.gene065797:CDS 45.0%
GGTATACCTGCTATGGCTTT+AGG - chr4.4:32925543-32925562 MS.gene065797:CDS 45.0%
! GATTGGGATTTGAAGCGGTA+CGG + chr4.4:32925022-32925041 None:intergenic 45.0%
! GGTTGGATTGGGATTTGAAG+CGG + chr4.4:32925027-32925046 None:intergenic 45.0%
! TGAAGCTAGTTTGACGACTC+GGG - chr4.4:32925833-32925852 MS.gene065797:CDS 45.0%
! TGAGAGTTTGGAACCGAACA+TGG - chr4.4:32925908-32925927 MS.gene065797:CDS 45.0%
! TGATGGTGTGATTGGTGTGA+GGG - chr4.4:32925986-32926005 MS.gene065797:CDS 45.0%
! TGGAGAGTCTTTTGCTTGGT+AGG - chr4.4:32925958-32925977 MS.gene065797:CDS 45.0%
!! CGTTGAAAAAGCGCTTCGTT+TGG - chr4.4:32925770-32925789 MS.gene065797:CDS 45.0%
!! TCGTTTTTGCGTTGTGGAGT+GGG + chr4.4:32925051-32925070 None:intergenic 45.0%
!! TTGATGGTGTGATTGGTGTG+AGG - chr4.4:32925985-32926004 MS.gene065797:CDS 45.0%
!!! CTGCTTTTTTGTCTCTGGCT+TGG - chr4.4:32926159-32926178 MS.gene065797:CDS 45.0%
GGTAAGAGAAGATCCGGTTG+TGG + chr4.4:32924994-32925013 None:intergenic 50.0%
GGTTGAGATGGAAATCGGAG+TGG + chr4.4:32924942-32924961 None:intergenic 50.0%
! AAACGACGATGTCGTTTCGG+TGG + chr4.4:32925082-32925101 None:intergenic 50.0%
! CAGGTGGAGAGTCTTTTGCT+TGG - chr4.4:32925954-32925973 MS.gene065797:CDS 50.0%
! TTTGGAACCGAACATGGCGT+TGG - chr4.4:32925914-32925933 MS.gene065797:CDS 50.0%
! TTTTTGCGTTGTGGAGTGGG+TGG + chr4.4:32925048-32925067 None:intergenic 50.0%
!!! GTCGTTTTTGCGTTGTGGAG+TGG + chr4.4:32925052-32925071 None:intergenic 50.0%
ATCCGGTTGTGGTGAGAGTC+GGG + chr4.4:32924983-32925002 None:intergenic 55.0%
CGGGTCGGGCATGATGAAAA+CGG + chr4.4:32924964-32924983 None:intergenic 55.0%
CTCTCCACCTGAAACCTCTC+CGG + chr4.4:32925945-32925964 None:intergenic 55.0%
GAAATCGGAGTGGCTATCGG+CGG + chr4.4:32924932-32924951 None:intergenic 55.0%
GATCCGGTTGTGGTGAGAGT+CGG + chr4.4:32924984-32925003 None:intergenic 55.0%
GATTGGTGTGAGGGAGAGGA+TGG - chr4.4:32925995-32926014 MS.gene065797:CDS 55.0%
GGCGAATCCAACGCCATGTT+CGG + chr4.4:32925924-32925943 None:intergenic 55.0%
GGGTCGGGCATGATGAAAAC+GGG + chr4.4:32924963-32924982 None:intergenic 55.0%
GTGGAGTGGGTGGTTGGATT+GGG + chr4.4:32925038-32925057 None:intergenic 55.0%
GTGTGATTGGTGTGAGGGAG+AGG - chr4.4:32925991-32926010 MS.gene065797:CDS 55.0%
TGTGGAGTGGGTGGTTGGAT+TGG + chr4.4:32925039-32925058 None:intergenic 55.0%
! ATTTGAAGCGGTACGGTGGC+GGG + chr4.4:32925015-32925034 None:intergenic 55.0%
! GAACATGGCGTTGGATTCGC+CGG - chr4.4:32925923-32925942 MS.gene065797:CDS 55.0%
! GATTTGAAGCGGTACGGTGG+CGG + chr4.4:32925016-32925035 None:intergenic 55.0%
! GCTAGTTTGACGACTCGGGT+TGG - chr4.4:32925837-32925856 MS.gene065797:CDS 55.0%
! TGGGATTTGAAGCGGTACGG+TGG + chr4.4:32925019-32925038 None:intergenic 55.0%
!! GTGGCGGGTAAGAGAAGATC+CGG + chr4.4:32925000-32925019 None:intergenic 55.0%
GACCCGACTCTCACCACAAC+CGG - chr4.4:32924978-32924997 MS.gene065797:CDS 60.0%
GCTATCGGCGGCGGACATTA+AGG + chr4.4:32924920-32924939 None:intergenic 60.0%
GGATTCGCCGGAGAGGTTTC+AGG - chr4.4:32925935-32925954 MS.gene065797:CDS 60.0%
GGTTGTGGTGAGAGTCGGGT+CGG + chr4.4:32924979-32924998 None:intergenic 60.0%
GTTGTGGTGAGAGTCGGGTC+GGG + chr4.4:32924978-32924997 None:intergenic 60.0%
TGCGTTGTGGAGTGGGTGGT+TGG + chr4.4:32925044-32925063 None:intergenic 60.0%
TGGTGTGAGGGAGAGGATGG+AGG - chr4.4:32925998-32926017 MS.gene065797:CDS 60.0%
TTCGCCGGAGAGGTTTCAGG+TGG - chr4.4:32925938-32925957 MS.gene065797:CDS 60.0%
ATCGGAGTGGCTATCGGCGG+CGG + chr4.4:32924929-32924948 None:intergenic 65.0%
! TGGCGTTGGATTCGCCGGAG+AGG - chr4.4:32925928-32925947 MS.gene065797:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 32924910 32926220 32924910 ID=MS.gene065797
chr4.4 mRNA 32924910 32926220 32924910 ID=MS.gene065797.t1;Parent=MS.gene065797
chr4.4 exon 32924910 32926220 32924910 ID=MS.gene065797.t1.exon1;Parent=MS.gene065797.t1
chr4.4 CDS 32924910 32926220 32924910 ID=cds.MS.gene065797.t1;Parent=MS.gene065797.t1
Gene Sequence

>MS.gene065797

ATGGATACCTTAATGTCCGCCGCCGATAGCCACTCCGATTTCCATCTCAACCCGTTTTCATCATGCCCGACCCGACTCTCACCACAACCGGATCTTCTCTTACCCGCCACCGTACCGCTTCAAATCCCAATCCAACCACCCACTCCACAACGCAAAAACGACACTAACACCACCGAAACGACATCGTCGTTTTCATCTTCCACAAACCCACTTCTTAAAACCCTAACCGAAATTGCTTCACTCATCGAAACCCAAAAACCAAATCAAGCAATTGAAACCCTAACCCATCTCAGTAAATCGATTTCACAAAACGGAAACCCTAATCAAAGAGTTTCCTTTTACTTTTCACAAGCCCTAACCAACAAAATCACCTCACAATCTTCAATAGCTTCTTCAAATTCTTCTTCAACTACTTGGGAAGAGTTAACACTCTCTTACAAAGCCTTAAACGATGCATGCCCATATTCAAAGTTCGCACATTTAACAGCAAATCAAGCAATTCTTGAAGCAACAGAAGGATCCAACAATATTCATATAGTTGATTTTGGAATTGTTCAAGGGATTCAATGGGCTGCATTGTTACAAGCATTTGCAACTCGTTCTTCTGGTAAACCTAATAGTGTAAGAATTTCTGGTATACCTGCTATGGCTTTAGGAACTTCGCCAGTTTCATCGATTTCAGCTACTGGTAATCGTTTATCTGAGTTTGCGAAACTTCTAGAGTTAAATTTTGAGTTTACACCGATTCTTACGCCTATTGAGCTTCTTGATGAATCAAGCTTTTGTATTCAGCCAAATGAGGCTTTAGCTGTTAATTTCATGCTTCAGTTGTATAATCTTTTGGATGAGAATATGAATTCCGTTGAAAAAGCGCTTCGTTTGGCGAAATCATTGAATCCTAAGATTGTTACTTTAGGTGAATATGAAGCTAGTTTGACGACTCGGGTTGGATTTGTTGAAAGATTTGAAACTGCTTTTAATTATTTTGCTGCTTTTTTTGAGAGTTTGGAACCGAACATGGCGTTGGATTCGCCGGAGAGGTTTCAGGTGGAGAGTCTTTTGCTTGGTAGGAGAATTGATGGTGTGATTGGTGTGAGGGAGAGGATGGAGGATAAAGAACAGTGGAAGGTTTTGATGGAAAGCTGTGGTTTTGAATCAGTGGGTTTGAGTCATTATGCAATTAGTCAAGCTAAGATATTGTTGTGGAATTATAGTTATAGTTCTTTGTATTCTCTTGTGGAATCTCAACCTGCTTTTTTGTCTCTGGCTTGGAAAGATGTTCCTCTATTGACTGTTTCTTCTTGGCGTTGA
Protein sequence

>MS.gene065797.t1

MDTLMSAADSHSDFHLNPFSSCPTRLSPQPDLLLPATVPLQIPIQPPTPQRKNDTNTTETTSSFSSSTNPLLKTLTEIASLIETQKPNQAIETLTHLSKSISQNGNPNQRVSFYFSQALTNKITSQSSIASSNSSSTTWEELTLSYKALNDACPYSKFAHLTANQAILEATEGSNNIHIVDFGIVQGIQWAALLQAFATRSSGKPNSVRISGIPAMALGTSPVSSISATGNRLSEFAKLLELNFEFTPILTPIELLDESSFCIQPNEALAVNFMLQLYNLLDENMNSVEKALRLAKSLNPKIVTLGEYEASLTTRVGFVERFETAFNYFAAFFESLEPNMALDSPERFQVESLLLGRRIDGVIGVRERMEDKEQWKVLMESCGFESVGLSHYAISQAKILLWNYSYSSLYSLVESQPAFLSLAWKDVPLLTVSSWR