AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065798

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065798.t1 MTR_4g077760 97.706 436 10 0 1 436 120 555 0.0 866
MS.gene065798.t1 MTR_2g082090 38.068 352 193 12 100 436 238 579 1.56e-54 191
MS.gene065798.t1 MTR_3g056110 35.876 354 210 10 94 436 195 542 2.22e-54 189
MS.gene065798.t1 MTR_5g094450 35.196 358 211 9 94 436 181 532 4.22e-54 189
MS.gene065798.t1 MTR_3g065980 35.401 387 214 13 71 436 169 540 5.22e-54 189
MS.gene065798.t1 MTR_7g074650 34.332 367 219 10 77 436 441 792 2.46e-53 191
MS.gene065798.t1 MTR_7g027190 33.065 372 210 9 90 436 316 673 2.71e-52 186
MS.gene065798.t1 MTR_3g089055 31.383 376 235 8 75 436 199 565 7.79e-51 181
MS.gene065798.t1 MTR_3g089055 31.383 376 235 8 75 436 204 570 1.04e-50 180
MS.gene065798.t1 MTR_1g029420 31.452 372 240 7 75 436 226 592 2.14e-50 180
MS.gene065798.t1 MTR_5g097480 33.684 380 237 9 67 436 170 544 2.34e-50 179
MS.gene065798.t1 MTR_1g069725 33.871 372 224 10 71 435 106 462 3.60e-50 177
MS.gene065798.t1 MTR_4g076020 32.778 360 200 13 88 436 101 429 6.40e-46 164
MS.gene065798.t1 MTR_3g072710 31.536 371 232 7 84 435 132 499 2.87e-45 164
MS.gene065798.t1 MTR_4g102790 30.287 383 239 12 71 435 289 661 5.10e-45 166
MS.gene065798.t1 MTR_4g104020 27.792 385 249 7 71 436 146 520 3.89e-44 161
MS.gene065798.t1 MTR_4g133660 32.286 350 220 9 97 435 199 542 5.81e-44 161
MS.gene065798.t1 MTR_1g096030 32.152 395 229 12 70 436 76 459 1.24e-43 159
MS.gene065798.t1 MTR_2g026250 31.161 353 222 10 97 436 252 596 8.65e-42 156
MS.gene065798.t1 MTR_4g097080 28.974 390 251 10 62 436 125 503 2.16e-40 151
MS.gene065798.t1 MTR_3g022830 28.009 432 239 13 65 436 17 436 1.46e-39 147
MS.gene065798.t1 MTR_5g058860 31.232 349 223 6 102 435 150 496 2.63e-39 148
MS.gene065798.t1 MTR_2g097310 28.684 380 244 10 72 435 269 637 1.17e-37 145
MS.gene065798.t1 MTR_4g064200 27.676 383 255 9 66 435 276 649 1.51e-37 145
MS.gene065798.t1 MTR_2g097410 26.772 381 254 10 72 435 368 740 3.72e-37 144
MS.gene065798.t1 MTR_4g064160 29.024 379 247 9 71 435 313 683 7.13e-37 143
MS.gene065798.t1 MTR_5g009080 25.688 436 244 9 71 436 53 478 1.00e-36 140
MS.gene065798.t1 MTR_8g442410 28.571 385 245 10 71 436 152 525 2.38e-36 140
MS.gene065798.t1 MTR_2g097350 28.421 380 245 10 72 435 271 639 3.53e-36 140
MS.gene065798.t1 MTR_4g064150 27.473 364 238 9 90 435 377 732 5.08e-36 141
MS.gene065798.t1 MTR_5g015490 29.517 393 241 12 67 436 111 490 7.75e-36 138
MS.gene065798.t1 MTR_2g097390 28.684 380 244 10 72 435 272 640 1.09e-35 139
MS.gene065798.t1 MTR_4g076140 26.545 437 238 12 71 436 45 469 1.12e-35 137
MS.gene065798.t1 MTR_8g093070 32.402 358 208 15 91 435 159 495 6.76e-35 135
MS.gene065798.t1 MTR_2g034250 27.062 388 252 11 71 436 207 585 1.23e-34 136
MS.gene065798.t1 MTR_4g064120 25.441 397 278 8 43 425 223 615 1.64e-34 136
MS.gene065798.t1 MTR_2g034280 26.702 382 258 9 71 436 200 575 1.74e-34 135
MS.gene065798.t1 MTR_2g097463 26.455 378 255 8 71 435 287 654 1.89e-34 136
MS.gene065798.t1 MTR_2g097467 25.464 377 260 7 71 435 286 653 2.83e-34 135
MS.gene065798.t1 MTR_2g034260 26.562 384 258 10 71 436 207 584 4.43e-34 134
MS.gene065798.t1 MTR_4g064180 27.429 350 236 8 90 425 270 615 5.00e-34 134
MS.gene065798.t1 MTR_4g026485 29.639 415 240 16 44 436 240 624 6.88e-34 134
MS.gene065798.t1 MTR_7g062120 26.703 367 246 9 89 436 378 740 3.10e-33 133
MS.gene065798.t1 MTR_2g097473 25.464 377 260 7 71 435 286 653 6.93e-33 131
MS.gene065798.t1 MTR_7g109580 29.076 368 244 10 79 436 194 554 1.85e-32 129
MS.gene065798.t1 MTR_2g097380 27.019 359 211 10 90 435 240 560 9.38e-32 127
MS.gene065798.t1 MTR_0092s0100 30.933 375 235 12 71 436 369 728 2.67e-31 127
MS.gene065798.t1 MTR_0092s0100 30.933 375 235 12 71 436 372 731 2.79e-31 127
MS.gene065798.t1 MTR_2g099110 27.735 393 241 11 73 436 73 451 4.93e-29 118
MS.gene065798.t1 MTR_2g089100 27.000 400 247 14 73 435 51 442 2.50e-26 110
MS.gene065798.t1 MTR_5g015950 24.429 438 263 13 61 435 13 445 4.45e-24 104
MS.gene065798.t1 MTR_3g053270 24.429 438 263 13 61 435 13 445 4.45e-24 104
MS.gene065798.t1 MTR_7g057230 29.594 419 251 17 39 435 260 656 1.04e-23 104
MS.gene065798.t1 MTR_4g095500 24.236 458 266 15 38 435 31 467 2.42e-20 93.6
MS.gene065798.t1 MTR_1g086970 21.271 362 251 10 96 436 130 478 1.46e-19 91.3
MS.gene065798.t1 MTR_8g020840 26.016 369 248 10 88 436 185 548 3.51e-19 90.5
MS.gene065798.t1 MTR_8g077940 25.770 357 217 13 102 436 211 541 7.91e-16 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065798.t1 AT5G66770 56.000 475 163 8 1 436 117 584 9.29e-166 479
MS.gene065798.t1 AT3G50650 59.947 377 131 5 70 436 176 542 1.43e-150 439
MS.gene065798.t1 AT5G41920 32.843 408 233 12 47 436 16 400 2.69e-56 191
MS.gene065798.t1 AT5G48150 34.646 381 233 10 67 436 115 490 3.96e-55 190
MS.gene065798.t1 AT5G48150 34.646 381 233 10 67 436 115 490 3.96e-55 190
MS.gene065798.t1 AT5G48150 34.646 381 233 10 67 436 115 490 3.96e-55 190
MS.gene065798.t1 AT5G48150 34.646 381 233 10 67 436 115 490 3.96e-55 190
MS.gene065798.t1 AT1G50600 35.000 380 232 9 67 436 152 526 1.73e-54 189
MS.gene065798.t1 AT3G03450 34.127 378 225 13 71 436 180 545 3.93e-54 189
MS.gene065798.t1 AT1G50600 35.000 380 232 9 67 436 227 601 6.75e-54 189
MS.gene065798.t1 AT1G50600 35.000 380 232 9 67 436 223 597 7.31e-54 189
MS.gene065798.t1 AT3G54220 33.875 369 219 10 77 435 296 649 2.02e-53 189
MS.gene065798.t1 AT2G04890 34.085 355 210 12 93 436 72 413 1.11e-50 177
MS.gene065798.t1 AT5G17490 32.061 393 244 12 54 436 137 516 3.59e-47 170
MS.gene065798.t1 AT1G66350 30.909 440 252 18 23 436 93 506 5.14e-46 166
MS.gene065798.t1 AT1G21450 28.244 393 267 8 54 436 206 593 7.62e-45 164
MS.gene065798.t1 AT1G63100 31.776 321 184 8 137 435 321 628 1.51e-44 164
MS.gene065798.t1 AT1G63100 31.776 321 184 8 137 435 346 653 2.74e-44 164
MS.gene065798.t1 AT2G01570 33.141 347 206 14 100 436 251 581 1.99e-43 160
MS.gene065798.t1 AT1G55580 31.842 380 204 9 104 436 74 445 2.48e-43 158
MS.gene065798.t1 AT4G17230 31.649 376 242 8 71 436 155 525 4.35e-43 159
MS.gene065798.t1 AT4G17230 31.649 376 242 8 71 436 155 525 4.35e-43 159
MS.gene065798.t1 AT1G14920 31.805 349 208 13 100 436 199 529 1.24e-42 157
MS.gene065798.t1 AT3G46600 27.763 371 255 7 75 436 5 371 4.43e-41 150
MS.gene065798.t1 AT4G08250 29.429 350 237 5 94 435 129 476 4.10e-40 150
MS.gene065798.t1 AT3G46600 27.763 371 255 7 75 436 181 547 7.45e-40 150
MS.gene065798.t1 AT3G46600 27.763 371 255 7 75 436 213 579 1.14e-39 150
MS.gene065798.t1 AT1G07520 28.729 362 239 7 90 435 170 528 4.15e-39 148
MS.gene065798.t1 AT1G07520 28.729 362 239 7 90 435 170 528 4.15e-39 148
MS.gene065798.t1 AT1G07520 28.729 362 239 7 90 435 334 692 1.07e-38 148
MS.gene065798.t1 AT1G07520 28.729 362 239 7 90 435 335 693 1.20e-38 148
MS.gene065798.t1 AT5G59450 26.847 406 273 9 48 436 200 598 2.67e-38 147
MS.gene065798.t1 AT4G37650 29.365 378 237 11 83 436 158 529 3.40e-38 145
MS.gene065798.t1 AT1G07530 26.633 398 270 9 52 435 375 764 5.99e-37 144
MS.gene065798.t1 AT2G29065 27.763 389 239 10 75 435 253 627 1.82e-36 142
MS.gene065798.t1 AT2G29060 25.178 421 296 10 29 435 239 654 5.40e-36 140
MS.gene065798.t1 AT2G29060 25.178 421 296 10 29 435 276 691 6.12e-36 140
MS.gene065798.t1 AT2G37650 25.217 345 236 7 105 436 378 713 5.17e-29 120
MS.gene065798.t1 AT2G37650 25.217 345 236 7 105 436 366 701 7.20e-29 120
MS.gene065798.t1 AT4G00150 28.338 367 224 13 86 436 211 554 1.08e-26 112
MS.gene065798.t1 AT5G52510 26.615 387 248 12 73 436 267 640 2.07e-26 112
MS.gene065798.t1 AT1G50420 31.878 229 135 7 85 305 71 286 6.94e-25 107
MS.gene065798.t1 AT1G50420 32.414 145 90 3 297 436 338 479 4.76e-15 77.4
MS.gene065798.t1 AT2G45160 28.070 399 227 16 71 436 268 639 1.40e-24 107
MS.gene065798.t1 AT3G60630 28.488 344 220 9 107 436 291 622 1.14e-23 104
MS.gene065798.t1 AT3G49950 28.011 357 223 12 104 435 60 407 1.16e-23 102
MS.gene065798.t1 AT3G13840 25.459 381 250 9 73 436 147 510 2.20e-20 94.0
MS.gene065798.t1 AT4G36710 23.947 380 259 12 71 436 122 485 8.31e-17 82.8

Find 87 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAATAGTGTAAGAATTTC+TGG 0.119456 4.4:+32959205 MS.gene065798:CDS
AATATTCATATAGTTGATTT+TGG 0.139541 4.4:+32959118 MS.gene065798:CDS
CTCTATTGACTGTTTCTTCT+TGG 0.170351 4.4:+32959875 MS.gene065798:CDS
CCAGAAATTCTTACACTATT+AGG 0.205458 4.4:-32959205 None:intergenic
ATATGGGCATGCATCGTTTA+AGG 0.214693 4.4:-32959035 None:intergenic
GCTTCAGTTGTATAATCTTT+TGG 0.233520 4.4:+32959414 MS.gene065798:CDS
TAAAAGGAAACTCTTTGATT+AGG 0.234171 4.4:-32958911 None:intergenic
GGTATACCTGCTATGGCTTT+AGG 0.252034 4.4:+32959226 MS.gene065798:CDS
TCACCTAAAGTAACAATCTT+AGG 0.256338 4.4:-32959490 None:intergenic
AAATGTGCGAACTTTGAATA+TGG 0.257358 4.4:-32959052 None:intergenic
AAAAGGAAACTCTTTGATTA+GGG 0.285344 4.4:-32958910 None:intergenic
AATCCTAAGATTGTTACTTT+AGG 0.287104 4.4:+32959487 MS.gene065798:CDS
TGTGGAGTGGGTGGTTGGAT+TGG 0.309124 4.4:-32958719 None:intergenic
AGGGTTTCAATTGCTTGATT+TGG 0.313945 4.4:-32958851 None:intergenic
GTGGAGTGGGTGGTTGGATT+GGG 0.321937 4.4:-32958718 None:intergenic
TTCGATGAGTGAAGCAATTT+CGG 0.323142 4.4:-32958819 None:intergenic
CAAATTCTTCTTCAACTACT+TGG 0.323189 4.4:+32958987 MS.gene065798:CDS
TAGGGCTTGTGAAAAGTAAA+AGG 0.330479 4.4:-32958927 None:intergenic
GGATTCGCCGGAGAGGTTTC+AGG 0.345000 4.4:+32959618 MS.gene065798:CDS
GCTATCGGCGGCGGACATTA+AGG 0.345403 4.4:-32958600 None:intergenic
TCGATTTACTGAGATGGGTT+AGG 0.351223 4.4:-32958871 None:intergenic
GCATTTGCAACTCGTTCTTC+TGG 0.357528 4.4:+32959178 MS.gene065798:CDS
TTAACAGCTAAAGCCTCATT+TGG 0.358736 4.4:-32959385 None:intergenic
ATCAACTATATGAATATTGT+TGG 0.382716 4.4:-32959113 None:intergenic
TAGTTCTTTGTATTCTCTTG+TGG 0.398410 4.4:+32959810 MS.gene065798:CDS
CGATTTACTGAGATGGGTTA+GGG 0.403048 4.4:-32958870 None:intergenic
TGAAAACGACGATGTCGTTT+CGG 0.406416 4.4:-32958765 None:intergenic
TTGCTTGGTAGGAGAATTGA+TGG 0.408569 4.4:+32959652 MS.gene065798:CDS
TGAGTGAAGCAATTTCGGTT+AGG 0.410733 4.4:-32958814 None:intergenic
GAGTGAAGCAATTTCGGTTA+GGG 0.413751 4.4:-32958813 None:intergenic
GAATTGTTCAAGGGATTCAA+TGG 0.414040 4.4:+32959140 MS.gene065798:CDS
GACCCGACTCTCACCACAAC+CGG 0.424081 4.4:+32958661 MS.gene065798:CDS
GGCGAATCCAACGCCATGTT+CGG 0.426377 4.4:-32959604 None:intergenic
ATTTGAAGCGGTACGGTGGC+GGG 0.431027 4.4:-32958695 None:intergenic
GATTCATCAAGAAGCTCAAT+AGG 0.436690 4.4:-32959346 None:intergenic
GATTTGAAGCGGTACGGTGG+CGG 0.443082 4.4:-32958696 None:intergenic
TGAAATCGATTTACTGAGAT+GGG 0.451988 4.4:-32958876 None:intergenic
ATCCGGTTGTGGTGAGAGTC+GGG 0.456439 4.4:-32958663 None:intergenic
GTAGCTGAAATCGATGAAAC+TGG 0.464413 4.4:-32959256 None:intergenic
TGCGTTGTGGAGTGGGTGGT+TGG 0.467268 4.4:-32958724 None:intergenic
GTGTGATTGGTGTGAGGGAG+AGG 0.470960 4.4:+32959674 MS.gene065798:CDS
AATTTCTGGTATACCTGCTA+TGG 0.471549 4.4:+32959219 MS.gene065798:CDS
GATTGGTGTGAGGGAGAGGA+TGG 0.472918 4.4:+32959678 MS.gene065798:CDS
AATGTGCGAACTTTGAATAT+GGG 0.473696 4.4:-32959051 None:intergenic
GTTTCATCGATTTCAGCTAC+TGG 0.480562 4.4:+32959259 MS.gene065798:CDS
GTGAAATCGATTTACTGAGA+TGG 0.489246 4.4:-32958877 None:intergenic
CGGGTCGGGCATGATGAAAA+CGG 0.497777 4.4:-32958644 None:intergenic
ATCGGAGTGGCTATCGGCGG+CGG 0.500643 4.4:-32958609 None:intergenic
AGGAGAATTGATGGTGTGAT+TGG 0.505945 4.4:+32959661 MS.gene065798:CDS
AAACGGGTTGAGATGGAAAT+CGG 0.511523 4.4:-32958627 None:intergenic
GGGTCGGGCATGATGAAAAC+GGG 0.514429 4.4:-32958643 None:intergenic
TGAGAGTTTGGAACCGAACA+TGG 0.520432 4.4:+32959591 MS.gene065798:CDS
AAATTCTTCTTCAACTACTT+GGG 0.520685 4.4:+32958988 MS.gene065798:CDS
TTTGGAACCGAACATGGCGT+TGG 0.523106 4.4:+32959597 MS.gene065798:CDS
GATTGGGATTTGAAGCGGTA+CGG 0.528703 4.4:-32958702 None:intergenic
ATGGAAATCGGAGTGGCTAT+CGG 0.534057 4.4:-32958615 None:intergenic
AATTGTTCAAGGGATTCAAT+GGG 0.536168 4.4:+32959141 MS.gene065798:CDS
GTTGTGGTGAGAGTCGGGTC+GGG 0.536815 4.4:-32958658 None:intergenic
GTGGCGGGTAAGAGAAGATC+CGG 0.537694 4.4:-32958680 None:intergenic
AGCTCAATAGGCGTAAGAAT+CGG 0.539095 4.4:-32959334 None:intergenic
TGAAGCTAGTTTGACGACTC+GGG 0.539102 4.4:+32959516 MS.gene065798:CDS
ATGAAGCTAGTTTGACGACT+CGG 0.543619 4.4:+32959515 MS.gene065798:CDS
GATCCGGTTGTGGTGAGAGT+CGG 0.547170 4.4:-32958664 None:intergenic
CAAGAAGAAACAGTCAATAG+AGG 0.549819 4.4:-32959874 None:intergenic
AGTAAATCGATTTCACAAAA+CGG 0.553570 4.4:+32958884 MS.gene065798:CDS
GAACATGGCGTTGGATTCGC+CGG 0.557782 4.4:+32959606 MS.gene065798:CDS
GCTAGTTTGACGACTCGGGT+TGG 0.562442 4.4:+32959520 MS.gene065798:CDS
GAAGTTCCTAAAGCCATAGC+AGG 0.575286 4.4:-32959232 None:intergenic
GGATGGAGGATAAAGAACAG+TGG 0.578860 4.4:+32959695 MS.gene065798:CDS
CTCTCCACCTGAAACCTCTC+CGG 0.581104 4.4:-32959625 None:intergenic
GGTTGTGGTGAGAGTCGGGT+CGG 0.584555 4.4:-32958659 None:intergenic
ATGATGAAAACGGGTTGAGA+TGG 0.594967 4.4:-32958634 None:intergenic
GGAGGATAAAGAACAGTGGA+AGG 0.599058 4.4:+32959699 MS.gene065798:CDS
GGTTGGATTGGGATTTGAAG+CGG 0.600133 4.4:-32958707 None:intergenic
TGGTGTGAGGGAGAGGATGG+AGG 0.600379 4.4:+32959681 MS.gene065798:CDS
GCAATTCTTGAAGCAACAGA+AGG 0.601796 4.4:+32959088 MS.gene065798:CDS
TGATGGTGTGATTGGTGTGA+GGG 0.605734 4.4:+32959669 MS.gene065798:CDS
GTCAAGCTAAGATATTGTTG+TGG 0.613839 4.4:+32959776 MS.gene065798:CDS
TGGGATTTGAAGCGGTACGG+TGG 0.615546 4.4:-32958699 None:intergenic
TTCGCCGGAGAGGTTTCAGG+TGG 0.626303 4.4:+32959621 MS.gene065798:CDS
GGTAAGAGAAGATCCGGTTG+TGG 0.627421 4.4:-32958674 None:intergenic
TGGCGTTGGATTCGCCGGAG+AGG 0.656610 4.4:+32959611 MS.gene065798:CDS
TTGATGGTGTGATTGGTGTG+AGG 0.659575 4.4:+32959668 MS.gene065798:CDS
GAAATCGGAGTGGCTATCGG+CGG 0.678873 4.4:-32958612 None:intergenic
AGAAGCTATTGAAGATTGTG+AGG 0.683841 4.4:-32958963 None:intergenic
AAACGACGATGTCGTTTCGG+TGG 0.687537 4.4:-32958762 None:intergenic
GGTTGAGATGGAAATCGGAG+TGG 0.716922 4.4:-32958622 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTCATATAGTTGATTT+TGG + chr4.4:32959118-32959137 MS.gene065798:CDS 15.0%
!! ATCAACTATATGAATATTGT+TGG - chr4.4:32959116-32959135 None:intergenic 20.0%
! AAAAGGAAACTCTTTGATTA+GGG - chr4.4:32958913-32958932 None:intergenic 25.0%
! AAATTCTTCTTCAACTACTT+GGG + chr4.4:32958988-32959007 MS.gene065798:CDS 25.0%
! TAAAAGGAAACTCTTTGATT+AGG - chr4.4:32958914-32958933 None:intergenic 25.0%
!! AGTAAATCGATTTCACAAAA+CGG + chr4.4:32958884-32958903 MS.gene065798:CDS 25.0%
!!! AATCCTAAGATTGTTACTTT+AGG + chr4.4:32959487-32959506 MS.gene065798:CDS 25.0%
!!! AATTGCTTGATTTGGTTTTT+GGG - chr4.4:32958846-32958865 None:intergenic 25.0%
!!! TTGATTTTGGAATTGTTCAA+GGG + chr4.4:32959131-32959150 MS.gene065798:CDS 25.0%
AAATGTGCGAACTTTGAATA+TGG - chr4.4:32959055-32959074 None:intergenic 30.0%
AATGTGCGAACTTTGAATAT+GGG - chr4.4:32959054-32959073 None:intergenic 30.0%
AATTGTTCAAGGGATTCAAT+GGG + chr4.4:32959141-32959160 MS.gene065798:CDS 30.0%
CAAATTCTTCTTCAACTACT+TGG + chr4.4:32958987-32959006 MS.gene065798:CDS 30.0%
CCAGAAATTCTTACACTATT+AGG - chr4.4:32959208-32959227 None:intergenic 30.0%
CCTAATAGTGTAAGAATTTC+TGG + chr4.4:32959205-32959224 MS.gene065798:CDS 30.0%
TAGTTCTTTGTATTCTCTTG+TGG + chr4.4:32959810-32959829 MS.gene065798:CDS 30.0%
TCACCTAAAGTAACAATCTT+AGG - chr4.4:32959493-32959512 None:intergenic 30.0%
! GCTTCAGTTGTATAATCTTT+TGG + chr4.4:32959414-32959433 MS.gene065798:CDS 30.0%
! TGAAATCGATTTACTGAGAT+GGG - chr4.4:32958879-32958898 None:intergenic 30.0%
!!! CAATTGCTTGATTTGGTTTT+TGG - chr4.4:32958847-32958866 None:intergenic 30.0%
!!! GTTGATTTTGGAATTGTTCA+AGG + chr4.4:32959130-32959149 MS.gene065798:CDS 30.0%
AGAAGCTATTGAAGATTGTG+AGG - chr4.4:32958966-32958985 None:intergenic 35.0%
AGGGTTTCAATTGCTTGATT+TGG - chr4.4:32958854-32958873 None:intergenic 35.0%
CAAGAAGAAACAGTCAATAG+AGG - chr4.4:32959877-32959896 None:intergenic 35.0%
GAATTGTTCAAGGGATTCAA+TGG + chr4.4:32959140-32959159 MS.gene065798:CDS 35.0%
GATTCATCAAGAAGCTCAAT+AGG - chr4.4:32959349-32959368 None:intergenic 35.0%
GTCAAGCTAAGATATTGTTG+TGG + chr4.4:32959776-32959795 MS.gene065798:CDS 35.0%
TAGGGCTTGTGAAAAGTAAA+AGG - chr4.4:32958930-32958949 None:intergenic 35.0%
TTAACAGCTAAAGCCTCATT+TGG - chr4.4:32959388-32959407 None:intergenic 35.0%
TTCGATGAGTGAAGCAATTT+CGG - chr4.4:32958822-32958841 None:intergenic 35.0%
! AGATTGTGAGGTGATTTTGT+TGG - chr4.4:32958954-32958973 None:intergenic 35.0%
! CTCTATTGACTGTTTCTTCT+TGG + chr4.4:32959875-32959894 MS.gene065798:CDS 35.0%
! CTTTTGTATTCAGCCAAATG+AGG + chr4.4:32959372-32959391 MS.gene065798:CDS 35.0%
! GTGAAATCGATTTACTGAGA+TGG - chr4.4:32958880-32958899 None:intergenic 35.0%
!! AATTTCTGGTATACCTGCTA+TGG + chr4.4:32959219-32959238 MS.gene065798:CDS 35.0%
!! TGAGGTGATTTTGTTGGTTA+GGG - chr4.4:32958948-32958967 None:intergenic 35.0%
!!! TGCTGCTTTTTTTGAGAGTT+TGG + chr4.4:32959579-32959598 MS.gene065798:CDS 35.0%
AGCTCAATAGGCGTAAGAAT+CGG - chr4.4:32959337-32959356 None:intergenic 40.0%
ATATGGGCATGCATCGTTTA+AGG - chr4.4:32959038-32959057 None:intergenic 40.0%
GAGTGAAGCAATTTCGGTTA+GGG - chr4.4:32958816-32958835 None:intergenic 40.0%
GCAATTCTTGAAGCAACAGA+AGG + chr4.4:32959088-32959107 MS.gene065798:CDS 40.0%
GTAGCTGAAATCGATGAAAC+TGG - chr4.4:32959259-32959278 None:intergenic 40.0%
TGAGTGAAGCAATTTCGGTT+AGG - chr4.4:32958817-32958836 None:intergenic 40.0%
TTGCTTGGTAGGAGAATTGA+TGG + chr4.4:32959652-32959671 MS.gene065798:CDS 40.0%
! ATGAAGCTAGTTTGACGACT+CGG + chr4.4:32959515-32959534 MS.gene065798:CDS 40.0%
! TCGATTTACTGAGATGGGTT+AGG - chr4.4:32958874-32958893 None:intergenic 40.0%
! TGAAAACGACGATGTCGTTT+CGG - chr4.4:32958768-32958787 None:intergenic 40.0%
!! AAACGGGTTGAGATGGAAAT+CGG - chr4.4:32958630-32958649 None:intergenic 40.0%
!! AAGGTTTTGATGGAAAGCTG+TGG + chr4.4:32959718-32959737 MS.gene065798:CDS 40.0%
!! AGAACAGTGGAAGGTTTTGA+TGG + chr4.4:32959708-32959727 MS.gene065798:CDS 40.0%
!! AGGAGAATTGATGGTGTGAT+TGG + chr4.4:32959661-32959680 MS.gene065798:CDS 40.0%
!! ATGATGAAAACGGGTTGAGA+TGG - chr4.4:32958637-32958656 None:intergenic 40.0%
!! CGATTTACTGAGATGGGTTA+GGG - chr4.4:32958873-32958892 None:intergenic 40.0%
!! GTGAGGTGATTTTGTTGGTT+AGG - chr4.4:32958949-32958968 None:intergenic 40.0%
!! GTTTCATCGATTTCAGCTAC+TGG + chr4.4:32959259-32959278 MS.gene065798:CDS 40.0%
!!! AAGCTGTGGTTTTGAATCAG+TGG + chr4.4:32959732-32959751 MS.gene065798:CDS 40.0%
!!! AGCTGTGGTTTTGAATCAGT+GGG + chr4.4:32959733-32959752 MS.gene065798:CDS 40.0%
!!! CAAACGAAGCGCTTTTTCAA+CGG - chr4.4:32959455-32959474 None:intergenic 40.0%
!!! CGGTTAGGGTTTTAAGAAGT+GGG - chr4.4:32958802-32958821 None:intergenic 40.0%
!!! GGTTTTAAGAAGTGGGTTTG+TGG - chr4.4:32958795-32958814 None:intergenic 40.0%
!!! TCAACCTGCTTTTTTGTCTC+TGG + chr4.4:32959837-32959856 MS.gene065798:CDS 40.0%
!!! TCGGTTAGGGTTTTAAGAAG+TGG - chr4.4:32958803-32958822 None:intergenic 40.0%
!!! TTAGTGTCGTTTTTGCGTTG+TGG - chr4.4:32958740-32958759 None:intergenic 40.0%
ATGGAAATCGGAGTGGCTAT+CGG - chr4.4:32958618-32958637 None:intergenic 45.0%
CAAGCCAGAGACAAAAAAGC+AGG - chr4.4:32959844-32959863 None:intergenic 45.0%
GAAGTTCCTAAAGCCATAGC+AGG - chr4.4:32959235-32959254 None:intergenic 45.0%
GCATTTGCAACTCGTTCTTC+TGG + chr4.4:32959178-32959197 MS.gene065798:CDS 45.0%
GGAGGATAAAGAACAGTGGA+AGG + chr4.4:32959699-32959718 MS.gene065798:CDS 45.0%
GGATGGAGGATAAAGAACAG+TGG + chr4.4:32959695-32959714 MS.gene065798:CDS 45.0%
GGTATACCTGCTATGGCTTT+AGG + chr4.4:32959226-32959245 MS.gene065798:CDS 45.0%
! GATTGGGATTTGAAGCGGTA+CGG - chr4.4:32958705-32958724 None:intergenic 45.0%
! GGTTGGATTGGGATTTGAAG+CGG - chr4.4:32958710-32958729 None:intergenic 45.0%
! TGAAGCTAGTTTGACGACTC+GGG + chr4.4:32959516-32959535 MS.gene065798:CDS 45.0%
! TGAGAGTTTGGAACCGAACA+TGG + chr4.4:32959591-32959610 MS.gene065798:CDS 45.0%
! TGATGGTGTGATTGGTGTGA+GGG + chr4.4:32959669-32959688 MS.gene065798:CDS 45.0%
! TGGAGAGTCTTTTGCTTGGT+AGG + chr4.4:32959641-32959660 MS.gene065798:CDS 45.0%
!! CGTTGAAAAAGCGCTTCGTT+TGG + chr4.4:32959453-32959472 MS.gene065798:CDS 45.0%
!! TCGTTTTTGCGTTGTGGAGT+GGG - chr4.4:32958734-32958753 None:intergenic 45.0%
!! TTGATGGTGTGATTGGTGTG+AGG + chr4.4:32959668-32959687 MS.gene065798:CDS 45.0%
!!! CTGCTTTTTTGTCTCTGGCT+TGG + chr4.4:32959842-32959861 MS.gene065798:CDS 45.0%
GGTAAGAGAAGATCCGGTTG+TGG - chr4.4:32958677-32958696 None:intergenic 50.0%
GGTTGAGATGGAAATCGGAG+TGG - chr4.4:32958625-32958644 None:intergenic 50.0%
! AAACGACGATGTCGTTTCGG+TGG - chr4.4:32958765-32958784 None:intergenic 50.0%
! CAGGTGGAGAGTCTTTTGCT+TGG + chr4.4:32959637-32959656 MS.gene065798:CDS 50.0%
! TTTGGAACCGAACATGGCGT+TGG + chr4.4:32959597-32959616 MS.gene065798:CDS 50.0%
! TTTTTGCGTTGTGGAGTGGG+TGG - chr4.4:32958731-32958750 None:intergenic 50.0%
!!! GTCGTTTTTGCGTTGTGGAG+TGG - chr4.4:32958735-32958754 None:intergenic 50.0%
ATCCGGTTGTGGTGAGAGTC+GGG - chr4.4:32958666-32958685 None:intergenic 55.0%
CGGGTCGGGCATGATGAAAA+CGG - chr4.4:32958647-32958666 None:intergenic 55.0%
CTCTCCACCTGAAACCTCTC+CGG - chr4.4:32959628-32959647 None:intergenic 55.0%
GAAATCGGAGTGGCTATCGG+CGG - chr4.4:32958615-32958634 None:intergenic 55.0%
GATCCGGTTGTGGTGAGAGT+CGG - chr4.4:32958667-32958686 None:intergenic 55.0%
GATTGGTGTGAGGGAGAGGA+TGG + chr4.4:32959678-32959697 MS.gene065798:CDS 55.0%
GGCGAATCCAACGCCATGTT+CGG - chr4.4:32959607-32959626 None:intergenic 55.0%
GGGTCGGGCATGATGAAAAC+GGG - chr4.4:32958646-32958665 None:intergenic 55.0%
GTGGAGTGGGTGGTTGGATT+GGG - chr4.4:32958721-32958740 None:intergenic 55.0%
GTGTGATTGGTGTGAGGGAG+AGG + chr4.4:32959674-32959693 MS.gene065798:CDS 55.0%
TGTGGAGTGGGTGGTTGGAT+TGG - chr4.4:32958722-32958741 None:intergenic 55.0%
! ATTTGAAGCGGTACGGTGGC+GGG - chr4.4:32958698-32958717 None:intergenic 55.0%
! GAACATGGCGTTGGATTCGC+CGG + chr4.4:32959606-32959625 MS.gene065798:CDS 55.0%
! GATTTGAAGCGGTACGGTGG+CGG - chr4.4:32958699-32958718 None:intergenic 55.0%
! GCTAGTTTGACGACTCGGGT+TGG + chr4.4:32959520-32959539 MS.gene065798:CDS 55.0%
! TGGGATTTGAAGCGGTACGG+TGG - chr4.4:32958702-32958721 None:intergenic 55.0%
!! GTGGCGGGTAAGAGAAGATC+CGG - chr4.4:32958683-32958702 None:intergenic 55.0%
GACCCGACTCTCACCACAAC+CGG + chr4.4:32958661-32958680 MS.gene065798:CDS 60.0%
GCTATCGGCGGCGGACATTA+AGG - chr4.4:32958603-32958622 None:intergenic 60.0%
GGATTCGCCGGAGAGGTTTC+AGG + chr4.4:32959618-32959637 MS.gene065798:CDS 60.0%
GGTTGTGGTGAGAGTCGGGT+CGG - chr4.4:32958662-32958681 None:intergenic 60.0%
GTTGTGGTGAGAGTCGGGTC+GGG - chr4.4:32958661-32958680 None:intergenic 60.0%
TGCGTTGTGGAGTGGGTGGT+TGG - chr4.4:32958727-32958746 None:intergenic 60.0%
TGGTGTGAGGGAGAGGATGG+AGG + chr4.4:32959681-32959700 MS.gene065798:CDS 60.0%
TTCGCCGGAGAGGTTTCAGG+TGG + chr4.4:32959621-32959640 MS.gene065798:CDS 60.0%
ATCGGAGTGGCTATCGGCGG+CGG - chr4.4:32958612-32958631 None:intergenic 65.0%
! TGGCGTTGGATTCGCCGGAG+AGG + chr4.4:32959611-32959630 MS.gene065798:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 32958593 32959903 32958593 ID=MS.gene065798
chr4.4 mRNA 32958593 32959903 32958593 ID=MS.gene065798.t1;Parent=MS.gene065798
chr4.4 exon 32958593 32959903 32958593 ID=MS.gene065798.t1.exon1;Parent=MS.gene065798.t1
chr4.4 CDS 32958593 32959903 32958593 ID=cds.MS.gene065798.t1;Parent=MS.gene065798.t1
Gene Sequence

>MS.gene065798

ATGGATACCTTAATGTCCGCCGCCGATAGCCACTCCGATTTCCATCTCAACCCGTTTTCATCATGCCCGACCCGACTCTCACCACAACCGGATCTTCTCTTACCCGCCACCGTACCGCTTCAAATCCCAATCCAACCACCCACTCCACAACGCAAAAACGACACTAACACCACCGAAACGACATCGTCGTTTTCATCTTCCACAAACCCACTTCTTAAAACCCTAACCGAAATTGCTTCACTCATCGAAACCCAAAAACCAAATCAAGCAATTGAAACCCTAACCCATCTCAGTAAATCGATTTCACAAAACGGAAACCCTAATCAAAGAGTTTCCTTTTACTTTTCACAAGCCCTAACCAACAAAATCACCTCACAATCTTCAATAGCTTCTTCAAATTCTTCTTCAACTACTTGGGAAGAGTTAACACTCTCTTACAAAGCCTTAAACGATGCATGCCCATATTCAAAGTTCGCACATTTAACAGCAAATCAAGCAATTCTTGAAGCAACAGAAGGATCCAACAATATTCATATAGTTGATTTTGGAATTGTTCAAGGGATTCAATGGGCTGCATTGTTACAAGCATTTGCAACTCGTTCTTCTGGTAAACCTAATAGTGTAAGAATTTCTGGTATACCTGCTATGGCTTTAGGAACTTCGCCAGTTTCATCGATTTCAGCTACTGGTAATCGTTTATCTGAGTTTGCGAAACTTCTAGAGTTAAATTTTGAGTTTACACCGATTCTTACGCCTATTGAGCTTCTTGATGAATCAAGCTTTTGTATTCAGCCAAATGAGGCTTTAGCTGTTAATTTCATGCTTCAGTTGTATAATCTTTTGGATGAGAATATGAATTCCGTTGAAAAAGCGCTTCGTTTGGCGAAATCATTGAATCCTAAGATTGTTACTTTAGGTGAATATGAAGCTAGTTTGACGACTCGGGTTGGATTTGTTGAAAGATTTGAAACTGCTTTTAATTATTTTGCTGCTTTTTTTGAGAGTTTGGAACCGAACATGGCGTTGGATTCGCCGGAGAGGTTTCAGGTGGAGAGTCTTTTGCTTGGTAGGAGAATTGATGGTGTGATTGGTGTGAGGGAGAGGATGGAGGATAAAGAACAGTGGAAGGTTTTGATGGAAAGCTGTGGTTTTGAATCAGTGGGTTTGAGTCATTATGCAATTAGTCAAGCTAAGATATTGTTGTGGAATTATAGTTATAGTTCTTTGTATTCTCTTGTGGAATCTCAACCTGCTTTTTTGTCTCTGGCTTGGAAAGATGTTCCTCTATTGACTGTTTCTTCTTGGCGTTGA
Protein sequence

>MS.gene065798.t1

MDTLMSAADSHSDFHLNPFSSCPTRLSPQPDLLLPATVPLQIPIQPPTPQRKNDTNTTETTSSFSSSTNPLLKTLTEIASLIETQKPNQAIETLTHLSKSISQNGNPNQRVSFYFSQALTNKITSQSSIASSNSSSTTWEELTLSYKALNDACPYSKFAHLTANQAILEATEGSNNIHIVDFGIVQGIQWAALLQAFATRSSGKPNSVRISGIPAMALGTSPVSSISATGNRLSEFAKLLELNFEFTPILTPIELLDESSFCIQPNEALAVNFMLQLYNLLDENMNSVEKALRLAKSLNPKIVTLGEYEASLTTRVGFVERFETAFNYFAAFFESLEPNMALDSPERFQVESLLLGRRIDGVIGVRERMEDKEQWKVLMESCGFESVGLSHYAISQAKILLWNYSYSSLYSLVESQPAFLSLAWKDVPLLTVSSWR