AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065883

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065883.t1 MTR_4g077760 97.127 557 14 1 1 557 1 555 0.0 1107
MS.gene065883.t1 MTR_2g082090 37.892 351 193 11 221 556 238 578 2.88e-53 191
MS.gene065883.t1 MTR_3g056110 35.593 354 211 10 215 557 195 542 5.92e-53 189
MS.gene065883.t1 MTR_5g094450 34.026 385 233 9 188 557 154 532 1.27e-52 187
MS.gene065883.t1 MTR_3g065980 34.884 387 216 12 192 557 169 540 2.50e-52 187
MS.gene065883.t1 MTR_7g074650 34.060 367 220 10 198 557 441 792 5.92e-52 190
MS.gene065883.t1 MTR_7g027190 31.529 425 235 11 158 557 280 673 1.02e-51 188
MS.gene065883.t1 MTR_3g089055 31.383 376 235 8 196 557 204 570 5.05e-50 181
MS.gene065883.t1 MTR_3g089055 31.383 376 235 8 196 557 199 565 6.24e-50 181
MS.gene065883.t1 MTR_1g069725 33.871 372 224 10 192 556 106 462 1.35e-49 178
MS.gene065883.t1 MTR_5g097480 33.684 380 237 9 188 557 170 544 3.11e-49 179
MS.gene065883.t1 MTR_1g029420 31.183 372 241 7 196 557 226 592 4.19e-49 179
MS.gene065883.t1 MTR_3g072710 31.536 371 232 7 205 556 132 499 3.25e-44 164
MS.gene065883.t1 MTR_4g102790 30.287 383 239 12 192 556 289 661 5.54e-44 166
MS.gene065883.t1 MTR_4g076020 31.944 360 203 12 209 557 101 429 6.24e-44 162
MS.gene065883.t1 MTR_4g104020 27.792 385 249 7 192 557 146 520 2.13e-43 162
MS.gene065883.t1 MTR_4g133660 32.286 350 220 9 218 556 199 542 3.93e-43 162
MS.gene065883.t1 MTR_1g096030 31.392 395 232 11 191 557 76 459 3.21e-42 157
MS.gene065883.t1 MTR_2g026250 30.571 350 228 8 218 557 252 596 3.94e-40 154
MS.gene065883.t1 MTR_4g097080 28.974 390 251 10 183 557 125 503 9.09e-40 152
MS.gene065883.t1 MTR_5g058860 31.232 349 223 6 223 556 150 496 1.50e-38 148
MS.gene065883.t1 MTR_3g022830 28.009 432 239 13 186 557 17 436 4.01e-38 145
MS.gene065883.t1 MTR_2g097310 28.684 380 244 10 193 556 269 637 2.18e-37 147
MS.gene065883.t1 MTR_4g064200 27.937 383 254 9 187 556 276 649 7.69e-37 145
MS.gene065883.t1 MTR_2g097410 26.772 381 254 10 193 556 368 740 1.68e-36 145
MS.gene065883.t1 MTR_4g064160 29.024 379 247 9 192 556 313 683 2.03e-36 144
MS.gene065883.t1 MTR_8g442410 28.571 385 245 10 192 557 152 525 6.02e-36 141
MS.gene065883.t1 MTR_4g064150 27.473 364 238 9 211 556 377 732 6.95e-36 143
MS.gene065883.t1 MTR_2g097350 28.421 380 245 10 193 556 271 639 8.76e-36 142
MS.gene065883.t1 MTR_5g015490 29.771 393 240 13 188 557 111 490 1.94e-35 139
MS.gene065883.t1 MTR_2g097390 28.684 380 244 10 193 556 272 640 2.08e-35 141
MS.gene065883.t1 MTR_5g009080 25.688 436 244 9 192 557 53 478 2.42e-35 139
MS.gene065883.t1 MTR_2g034250 27.062 388 252 11 192 557 207 585 1.05e-34 138
MS.gene065883.t1 MTR_8g093070 30.730 397 245 15 172 556 117 495 1.78e-34 137
MS.gene065883.t1 MTR_2g097467 25.729 377 259 7 192 556 286 653 2.31e-34 138
MS.gene065883.t1 MTR_4g076140 26.545 437 238 12 192 557 45 469 3.05e-34 135
MS.gene065883.t1 MTR_4g064120 25.693 397 277 8 164 546 223 615 3.33e-34 137
MS.gene065883.t1 MTR_2g034280 26.579 380 261 8 192 557 200 575 3.64e-34 137
MS.gene065883.t1 MTR_2g034260 26.562 384 258 10 192 557 207 584 3.74e-34 137
MS.gene065883.t1 MTR_2g097463 25.381 394 271 8 176 556 271 654 3.81e-34 137
MS.gene065883.t1 MTR_4g064180 27.714 350 235 8 211 546 270 615 1.21e-33 135
MS.gene065883.t1 MTR_4g026485 29.639 415 240 16 165 557 240 624 7.01e-33 133
MS.gene065883.t1 MTR_2g097473 25.729 377 259 7 192 556 286 653 8.37e-33 133
MS.gene065883.t1 MTR_7g062120 26.836 354 241 8 218 557 391 740 1.34e-32 133
MS.gene065883.t1 MTR_7g109580 29.076 368 244 10 200 557 194 554 2.80e-32 131
MS.gene065883.t1 MTR_0092s0100 30.667 375 236 12 192 557 369 728 7.14e-31 128
MS.gene065883.t1 MTR_0092s0100 30.667 375 236 12 192 557 372 731 7.33e-31 128
MS.gene065883.t1 MTR_2g097380 27.019 359 211 10 211 556 240 560 9.50e-31 126
MS.gene065883.t1 MTR_2g099110 27.735 393 241 11 194 557 73 451 9.45e-29 119
MS.gene065883.t1 MTR_2g089100 26.316 399 251 13 194 556 51 442 9.36e-26 110
MS.gene065883.t1 MTR_5g015950 24.429 438 263 13 182 556 13 445 3.75e-23 102
MS.gene065883.t1 MTR_3g053270 24.429 438 263 13 182 556 13 445 3.75e-23 102
MS.gene065883.t1 MTR_7g057230 29.017 417 256 16 160 556 260 656 4.14e-23 103
MS.gene065883.t1 MTR_4g095500 23.673 452 276 14 159 556 31 467 8.55e-20 92.8
MS.gene065883.t1 MTR_1g086970 21.978 364 246 12 217 557 130 478 4.72e-19 90.5
MS.gene065883.t1 MTR_8g020840 25.260 384 258 10 196 557 172 548 7.63e-19 90.5
MS.gene065883.t1 MTR_8g077940 25.770 357 217 13 223 557 211 541 6.14e-16 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065883.t1 AT5G66770 54.023 609 203 13 1 557 1 584 0.0 538
MS.gene065883.t1 AT3G50650 49.915 585 222 14 1 557 1 542 2.69e-162 473
MS.gene065883.t1 AT5G41920 32.843 408 233 12 168 557 16 400 7.95e-55 191
MS.gene065883.t1 AT5G48150 34.383 381 234 9 188 557 115 490 5.88e-53 187
MS.gene065883.t1 AT5G48150 34.383 381 234 9 188 557 115 490 5.88e-53 187
MS.gene065883.t1 AT5G48150 34.383 381 234 9 188 557 115 490 5.88e-53 187
MS.gene065883.t1 AT5G48150 34.383 381 234 9 188 557 115 490 5.88e-53 187
MS.gene065883.t1 AT3G03450 31.410 468 279 18 117 557 93 545 1.02e-52 188
MS.gene065883.t1 AT1G50600 34.211 380 235 8 188 557 152 526 1.44e-52 187
MS.gene065883.t1 AT3G54220 33.784 370 218 11 198 556 296 649 5.13e-52 188
MS.gene065883.t1 AT1G50600 34.211 380 235 8 188 557 223 597 5.25e-52 187
MS.gene065883.t1 AT1G50600 34.211 380 235 8 188 557 227 601 7.34e-52 187
MS.gene065883.t1 AT2G04890 34.085 355 210 12 214 557 72 413 4.94e-49 175
MS.gene065883.t1 AT5G17490 32.061 393 244 12 175 557 137 516 9.57e-46 169
MS.gene065883.t1 AT1G66350 29.149 470 276 16 110 557 72 506 6.36e-45 166
MS.gene065883.t1 AT1G21450 28.250 400 272 8 168 557 199 593 7.90e-45 167
MS.gene065883.t1 AT1G63100 31.776 321 184 8 258 556 321 628 4.98e-43 163
MS.gene065883.t1 AT1G63100 31.949 313 178 8 266 556 354 653 5.37e-43 163
MS.gene065883.t1 AT4G17230 31.649 376 242 8 192 557 155 525 4.37e-42 159
MS.gene065883.t1 AT4G17230 31.649 376 242 8 192 557 155 525 4.37e-42 159
MS.gene065883.t1 AT2G01570 33.141 347 206 14 221 557 251 581 1.15e-41 158
MS.gene065883.t1 AT1G55580 31.579 380 205 9 225 557 74 445 3.42e-41 154
MS.gene065883.t1 AT1G14920 31.232 349 210 12 221 557 199 529 2.60e-40 154
MS.gene065883.t1 AT3G46600 27.763 371 255 7 196 557 5 371 1.58e-39 148
MS.gene065883.t1 AT4G08250 26.707 498 328 12 74 556 1 476 1.06e-38 148
MS.gene065883.t1 AT3G46600 27.763 371 255 7 196 557 213 579 1.36e-38 149
MS.gene065883.t1 AT3G46600 27.763 371 255 7 196 557 181 547 2.04e-38 149
MS.gene065883.t1 AT4G37650 29.101 378 238 11 204 557 158 529 8.68e-38 146
MS.gene065883.t1 AT1G07520 28.382 377 251 7 196 556 319 692 8.92e-38 148
MS.gene065883.t1 AT1G07520 28.382 377 251 7 196 556 320 693 9.40e-38 148
MS.gene065883.t1 AT1G07520 28.382 377 251 7 196 556 155 528 1.01e-37 146
MS.gene065883.t1 AT1G07520 28.382 377 251 7 196 556 155 528 1.01e-37 146
MS.gene065883.t1 AT5G59450 26.551 403 278 8 169 557 200 598 1.05e-37 147
MS.gene065883.t1 AT1G07530 26.633 398 270 9 173 556 375 764 1.86e-36 145
MS.gene065883.t1 AT2G29065 27.763 389 239 10 196 556 253 627 9.36e-36 142
MS.gene065883.t1 AT2G29060 24.524 420 300 9 150 556 276 691 3.14e-35 141
MS.gene065883.t1 AT2G29060 24.524 420 300 9 150 556 239 654 4.01e-35 140
MS.gene065883.t1 AT2G37650 25.217 345 236 7 226 557 366 701 5.19e-28 119
MS.gene065883.t1 AT2G37650 25.217 345 236 7 226 557 378 713 5.24e-28 119
MS.gene065883.t1 AT5G52510 26.615 387 248 12 194 557 267 640 2.45e-26 114
MS.gene065883.t1 AT4G00150 28.302 371 219 14 207 557 211 554 6.63e-26 112
MS.gene065883.t1 AT1G50420 31.278 227 139 5 206 426 71 286 6.78e-24 105
MS.gene065883.t1 AT1G50420 32.414 145 90 3 418 557 338 479 6.68e-15 77.8
MS.gene065883.t1 AT2G45160 28.070 399 227 16 192 557 268 639 1.14e-23 105
MS.gene065883.t1 AT3G60630 28.488 344 220 9 228 557 291 622 1.74e-23 105
MS.gene065883.t1 AT3G49950 27.171 357 226 12 225 556 60 407 1.22e-22 100
MS.gene065883.t1 AT3G13840 25.722 381 249 9 194 557 147 510 7.24e-21 96.7
MS.gene065883.t1 AT4G36710 23.947 380 259 12 192 557 122 485 4.10e-17 84.7

Find 125 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAATAGTGTAAGAATTTC+TGG 0.119456 4.2:+32080190 MS.gene065883:CDS
GATATTCATATAGTTGATTT+TGG 0.147557 4.2:+32080103 MS.gene065883:CDS
CTCTATTGACTGTTTCTTCT+TGG 0.170351 4.2:+32080860 MS.gene065883:CDS
CCAGAAATTCTTACACTATT+AGG 0.205458 4.2:-32080190 None:intergenic
ATATGGGCATGCATCGTTTA+AGG 0.214693 4.2:-32080020 None:intergenic
GCTTCAGTTGTATAATCTTT+TGG 0.233520 4.2:+32080399 MS.gene065883:CDS
TAAAAGGAAACTCTTTGATT+AGG 0.234171 4.2:-32079896 None:intergenic
GGTATACCTGCTATGGCTTT+AGG 0.252034 4.2:+32080211 MS.gene065883:CDS
TCACCTAAAGTAACAATCTT+AGG 0.256338 4.2:-32080475 None:intergenic
AAATGTGCGAACTTTGAATA+TGG 0.257358 4.2:-32080037 None:intergenic
TGGGTGGTTGGATTGGGATT+TGG 0.269914 4.2:-32079697 None:intergenic
AAAAGGAAACTCTTTGATTA+GGG 0.285344 4.2:-32079895 None:intergenic
AATCCTAAGATTGTTACTTT+AGG 0.287104 4.2:+32080472 MS.gene065883:CDS
AGGGTTTCAATTGCTTGATT+TGG 0.313945 4.2:-32079836 None:intergenic
TAAGGCTTGTGAAAAGTAAA+AGG 0.314801 4.2:-32079912 None:intergenic
TTCGATGAGTGAAGCAATTT+CGG 0.323142 4.2:-32079804 None:intergenic
CTTGTTGAGCTATTGCCATT+AGG 0.345192 4.2:-32079244 None:intergenic
TGCGGAGTGGGTGGTTGGAT+TGG 0.345602 4.2:-32079704 None:intergenic
GCGGAGTGGGTGGTTGGATT+GGG 0.350584 4.2:-32079703 None:intergenic
TTGATTTGCTGAGATGGGTT+AGG 0.352847 4.2:-32079856 None:intergenic
TTGTGATACCTAGGATGTTT+TGG 0.353661 4.2:-32079331 None:intergenic
GCATTTGCAACTCGTTCTTC+TGG 0.357528 4.2:+32080163 MS.gene065883:CDS
TTAACAGCTAAAGCCTCATT+TGG 0.363944 4.2:-32080370 None:intergenic
TTGAAATGGGTCGGTGAAAT+CGG 0.367983 4.2:-32079450 None:intergenic
GGACCCGACCCGACTTGAAA+TGG 0.372343 4.2:-32079464 None:intergenic
GACCCGACTTTCGCCGCAAC+CGG 0.383498 4.2:+32079646 MS.gene065883:CDS
CCAGTCATCGGAGTCGAAAT+CGG 0.397137 4.2:-32079555 None:intergenic
TAGTTCTTTGTATTCTCTTG+TGG 0.398410 4.2:+32080795 MS.gene065883:CDS
CCGATTTCGACTCCGATGAC+TGG 0.399290 4.2:+32079555 MS.gene065883:CDS
TGATTTGCTGAGATGGGTTA+GGG 0.400674 4.2:-32079855 None:intergenic
CAAATTCTTCTTCTACTACA+TGG 0.403280 4.2:+32079972 MS.gene065883:CDS
ATTTGGAGTGGCACGGTGGC+GGG 0.405277 4.2:-32079680 None:intergenic
TGAAAACGACGATGTCGTTT+CGG 0.406416 4.2:-32079750 None:intergenic
TTGCTTGGTAGGAGAATTGA+TGG 0.408569 4.2:+32080637 MS.gene065883:CDS
TGAGTGAAGCAATTTCGGTT+AGG 0.410733 4.2:-32079799 None:intergenic
GAGTGAAGCAATTTCGGTTA+GGG 0.413751 4.2:-32079798 None:intergenic
GAATTGTTCAAGGGATTCAA+TGG 0.414040 4.2:+32080125 MS.gene065883:CDS
ATCCGGTTGCGGCGAAAGTC+GGG 0.417122 4.2:-32079648 None:intergenic
GATTTGGAGTGGCACGGTGG+CGG 0.424354 4.2:-32079681 None:intergenic
AGTGATGAAGAAGAAGATAC+TGG 0.431284 4.2:-32079404 None:intergenic
GATTCATCAAGAAGCTCAAT+AGG 0.436690 4.2:-32080331 None:intergenic
GTGAAATTGATTTGCTGAGA+TGG 0.442057 4.2:-32079862 None:intergenic
GGCGAATCCAACGCCATGTT+CGG 0.446437 4.2:-32080589 None:intergenic
TTTGGTGATGATGATGTTGT+TGG 0.448206 4.2:-32079313 None:intergenic
GTTGTCGGCGGCGGACATTA+AGG 0.450739 4.2:-32079585 None:intergenic
AAACTCAGATAAACGATTAC+CGG 0.454956 4.2:-32080263 None:intergenic
TCACCGACCCATTTCAAGTC+GGG 0.456993 4.2:+32079456 MS.gene065883:CDS
TTCGTTGCGGAGTGGGTGGT+TGG 0.460150 4.2:-32079709 None:intergenic
ATCGGAGTGGTTGTCGGCGG+CGG 0.468764 4.2:-32079594 None:intergenic
GTGTGATTGGTGTGAGGGAG+AGG 0.470960 4.2:+32080659 MS.gene065883:CDS
GTAGCTGAAATCGATGAAAC+TGG 0.471542 4.2:-32080241 None:intergenic
AATTTCTGGTATACCTGCTA+TGG 0.471549 4.2:+32080204 MS.gene065883:CDS
GATTGGTGTGAGGGAGAGGA+TGG 0.472918 4.2:+32080663 MS.gene065883:CDS
AATGTGCGAACTTTGAATAT+GGG 0.473696 4.2:-32080036 None:intergenic
GACCCATTTCAAGTCGGGTC+GGG 0.475045 4.2:+32079461 MS.gene065883:CDS
AGCAAATCAATTTCACAAAA+CGG 0.480108 4.2:+32079869 MS.gene065883:CDS
ATCAACTATATGAATATCGT+TGG 0.482770 4.2:-32080098 None:intergenic
GTTGCGGCGAAAGTCGGGTC+GGG 0.485945 4.2:-32079643 None:intergenic
CTCTCCACTTGAAACCTCTC+TGG 0.491073 4.2:-32080610 None:intergenic
ATGGAAATCGGAGTGGTTGT+CGG 0.492567 4.2:-32079600 None:intergenic
AAATTCTTCTTCTACTACAT+GGG 0.496572 4.2:+32079973 MS.gene065883:CDS
CGGGTCGGGCATGATGAAAA+CGG 0.497777 4.2:-32079629 None:intergenic
GATCCGGTTGCGGCGAAAGT+CGG 0.502966 4.2:-32079649 None:intergenic
TGAAATTGATTTGCTGAGAT+GGG 0.503445 4.2:-32079861 None:intergenic
AGGAGAATTGATGGTGTGAT+TGG 0.505945 4.2:+32080646 MS.gene065883:CDS
GACCCGACCCGACTTGAAAT+GGG 0.507947 4.2:-32079463 None:intergenic
GAGTGAGGGTCGAGTGGTGA+AGG 0.509192 4.2:-32079515 None:intergenic
AAACGGGTTGAGATGGAAAT+CGG 0.511523 4.2:-32079612 None:intergenic
CACTGACAGTGGAAACCTAA+TGG 0.513258 4.2:+32079229 MS.gene065883:CDS
GGGTCGGGCATGATGAAAAC+GGG 0.514429 4.2:-32079628 None:intergenic
TTTGGAGCCGAACATGGCGT+TGG 0.516288 4.2:+32080582 MS.gene065883:CDS
CGGAGTCGAAATCGGAGAAA+CGG 0.521347 4.2:-32079547 None:intergenic
CATCATCACCAAAACATCCT+AGG 0.524123 4.2:+32079323 MS.gene065883:CDS
GGTTGGATTGGGATTTGGAG+TGG 0.534821 4.2:-32079692 None:intergenic
AATTGTTCAAGGGATTCAAT+GGG 0.536168 4.2:+32080126 MS.gene065883:CDS
AGCTCAATAGGCGTAAGAAT+CGG 0.539095 4.2:-32080319 None:intergenic
TGAAGCTAGTTTGACGACTC+GGG 0.539102 4.2:+32080501 MS.gene065883:CDS
ATGAAGCTAGTTTGACGACT+CGG 0.543619 4.2:+32080500 MS.gene065883:CDS
CGATGTCGTTTCGGTGGTGT+TGG 0.543827 4.2:-32079741 None:intergenic
TTTCGACTCCGATGACTGGA+TGG 0.549581 4.2:+32079559 MS.gene065883:CDS
CAAGAAGAAACAGTCAATAG+AGG 0.549819 4.2:-32080859 None:intergenic
TGAGAGTTTGGAGCCGAACA+TGG 0.552448 4.2:+32080576 MS.gene065883:CDS
GTGGCGGGTAGGAGAAGATC+CGG 0.554130 4.2:-32079665 None:intergenic
GCTAGTTTGACGACTCGGGT+TGG 0.562442 4.2:+32080505 MS.gene065883:CDS
CGACCCATTTCAAGTCGGGT+CGG 0.565104 4.2:+32079460 MS.gene065883:CDS
TAAGGTATCCATCCAGTCAT+CGG 0.565279 4.2:-32079567 None:intergenic
GTTTCATCGATTTCAGCTAC+CGG 0.566724 4.2:+32080244 MS.gene065883:CDS
GAAGTTCCTAAAGCCATAGC+AGG 0.567270 4.2:-32080217 None:intergenic
TCTCTGTCTCACTACATCCA+TGG 0.569966 4.2:+32079360 MS.gene065883:CDS
GGAGTGGCACGGTGGCGGGT+AGG 0.573432 4.2:-32079676 None:intergenic
AAACGGAAGGTGGTTGAGTG+AGG 0.573544 4.2:-32079530 None:intergenic
GGATGGAGGATAAAGAACAG+TGG 0.578860 4.2:+32080680 MS.gene065883:CDS
GGTGAAGGAAAGTTGAAAGC+GGG 0.586484 4.2:-32079500 None:intergenic
TTCGCCAGAGAGGTTTCAAG+TGG 0.588796 4.2:+32080606 MS.gene065883:CDS
AGGAAAGTTGAAAGCGGGAT+CGG 0.590312 4.2:-32079495 None:intergenic
AGAGAGGGTTTGTGATACCT+AGG 0.592419 4.2:-32079340 None:intergenic
GATTGGGATTTGGAGTGGCA+CGG 0.594042 4.2:-32079687 None:intergenic
ATGATGAAAACGGGTTGAGA+TGG 0.594967 4.2:-32079619 None:intergenic
GGAGGATAAAGAACAGTGGA+AGG 0.599058 4.2:+32080684 MS.gene065883:CDS
TGGTGTGAGGGAGAGGATGG+AGG 0.600379 4.2:+32080666 MS.gene065883:CDS
GGATGTAGTGAGACAGAGAG+AGG 0.601119 4.2:-32079356 None:intergenic
TTCACCGACCCATTTCAAGT+CGG 0.604924 4.2:+32079455 MS.gene065883:CDS
TGATGGTGTGATTGGTGTGA+GGG 0.605734 4.2:+32080654 MS.gene065883:CDS
GGTTGCGGCGAAAGTCGGGT+CGG 0.606467 4.2:-32079644 None:intergenic
AACGGAAGGTGGTTGAGTGA+GGG 0.606602 4.2:-32079529 None:intergenic
GTGGTTGAGTGAGGGTCGAG+TGG 0.609203 4.2:-32079521 None:intergenic
GTCAAGCTAAGATATTGTTG+TGG 0.613839 4.2:+32080761 MS.gene065883:CDS
AATGGGTCGGTGAAATCGGT+AGG 0.625917 4.2:-32079446 None:intergenic
GCGATTCTTGAAGCAACAGA+AGG 0.626009 4.2:+32080073 MS.gene065883:CDS
GATGTAGTGAGACAGAGAGA+GGG 0.639502 4.2:-32079355 None:intergenic
GGTAGGAGAAGATCCGGTTG+CGG 0.644273 4.2:-32079659 None:intergenic
GGGTCGGTGAAATCGGTAGG+TGG 0.649301 4.2:-32079443 None:intergenic
GTCGAAATCGGAGAAACGGA+AGG 0.650056 4.2:-32079543 None:intergenic
GAAATCGGAGTGGTTGTCGG+CGG 0.651663 4.2:-32079597 None:intergenic
GCTTACATGTGCACTGACAG+TGG 0.657212 4.2:+32079218 MS.gene065883:CDS
GAAATCGGAGAAACGGAAGG+TGG 0.658200 4.2:-32079540 None:intergenic
TTGATGGTGTGATTGGTGTG+AGG 0.659575 4.2:+32080653 MS.gene065883:CDS
CGACCCGACTTGAAATGGGT+CGG 0.673110 4.2:-32079459 None:intergenic
TCGGTAGGTGGAAAAGACAA+AGG 0.681946 4.2:-32079431 None:intergenic
TGGGATTTGGAGTGGCACGG+TGG 0.682217 4.2:-32079684 None:intergenic
TGGTGAAGGAAAGTTGAAAG+CGG 0.682780 4.2:-32079501 None:intergenic
TGGCGTTGGATTCGCCAGAG+AGG 0.687403 4.2:+32080596 MS.gene065883:CDS
AAACGACGATGTCGTTTCGG+TGG 0.687537 4.2:-32079747 None:intergenic
GTAGGTGGAAAAGACAAAGG+TGG 0.720227 4.2:-32079428 None:intergenic
GGTTGAGATGGAAATCGGAG+TGG 0.720412 4.2:-32079607 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATATTCATATAGTTGATTT+TGG + chr4.2:32080103-32080122 MS.gene065883:CDS 20.0%
! AAAAGGAAACTCTTTGATTA+GGG - chr4.2:32079898-32079917 None:intergenic 25.0%
! AAATTCTTCTTCTACTACAT+GGG + chr4.2:32079973-32079992 MS.gene065883:CDS 25.0%
! AGCAAATCAATTTCACAAAA+CGG + chr4.2:32079869-32079888 MS.gene065883:CDS 25.0%
! ATCAACTATATGAATATCGT+TGG - chr4.2:32080101-32080120 None:intergenic 25.0%
! TAAAAGGAAACTCTTTGATT+AGG - chr4.2:32079899-32079918 None:intergenic 25.0%
!!! AATCCTAAGATTGTTACTTT+AGG + chr4.2:32080472-32080491 MS.gene065883:CDS 25.0%
!!! AATTGCTTGATTTGGTTTTT+GGG - chr4.2:32079831-32079850 None:intergenic 25.0%
!!! TTGATTTTGGAATTGTTCAA+GGG + chr4.2:32080116-32080135 MS.gene065883:CDS 25.0%
AAACTCAGATAAACGATTAC+CGG - chr4.2:32080266-32080285 None:intergenic 30.0%
AAATGTGCGAACTTTGAATA+TGG - chr4.2:32080040-32080059 None:intergenic 30.0%
AATGTGCGAACTTTGAATAT+GGG - chr4.2:32080039-32080058 None:intergenic 30.0%
AATTGTTCAAGGGATTCAAT+GGG + chr4.2:32080126-32080145 MS.gene065883:CDS 30.0%
CAAATTCTTCTTCTACTACA+TGG + chr4.2:32079972-32079991 MS.gene065883:CDS 30.0%
CCAGAAATTCTTACACTATT+AGG - chr4.2:32080193-32080212 None:intergenic 30.0%
CCTAATAGTGTAAGAATTTC+TGG + chr4.2:32080190-32080209 MS.gene065883:CDS 30.0%
TAAGGCTTGTGAAAAGTAAA+AGG - chr4.2:32079915-32079934 None:intergenic 30.0%
TAGTTCTTTGTATTCTCTTG+TGG + chr4.2:32080795-32080814 MS.gene065883:CDS 30.0%
TCACCTAAAGTAACAATCTT+AGG - chr4.2:32080478-32080497 None:intergenic 30.0%
! CTTTTGTATTCAACCAAATG+AGG + chr4.2:32080357-32080376 MS.gene065883:CDS 30.0%
! GCTTCAGTTGTATAATCTTT+TGG + chr4.2:32080399-32080418 MS.gene065883:CDS 30.0%
!! TGAAATTGATTTGCTGAGAT+GGG - chr4.2:32079864-32079883 None:intergenic 30.0%
!!! CAATTGCTTGATTTGGTTTT+TGG - chr4.2:32079832-32079851 None:intergenic 30.0%
!!! GTTGATTTTGGAATTGTTCA+AGG + chr4.2:32080115-32080134 MS.gene065883:CDS 30.0%
!!! GTTGTTCTTGTTGTTGTTTT+TGG - chr4.2:32079277-32079296 None:intergenic 30.0%
AGGGTTTCAATTGCTTGATT+TGG - chr4.2:32079839-32079858 None:intergenic 35.0%
AGTGATGAAGAAGAAGATAC+TGG - chr4.2:32079407-32079426 None:intergenic 35.0%
CAAGAAGAAACAGTCAATAG+AGG - chr4.2:32080862-32080881 None:intergenic 35.0%
GAATTGTTCAAGGGATTCAA+TGG + chr4.2:32080125-32080144 MS.gene065883:CDS 35.0%
GATTCATCAAGAAGCTCAAT+AGG - chr4.2:32080334-32080353 None:intergenic 35.0%
GTCAAGCTAAGATATTGTTG+TGG + chr4.2:32080761-32080780 MS.gene065883:CDS 35.0%
TTAACAGCTAAAGCCTCATT+TGG - chr4.2:32080373-32080392 None:intergenic 35.0%
TTCGATGAGTGAAGCAATTT+CGG - chr4.2:32079807-32079826 None:intergenic 35.0%
! AGATTGTGCAGTGATTTTGT+TGG - chr4.2:32079939-32079958 None:intergenic 35.0%
! CTCTATTGACTGTTTCTTCT+TGG + chr4.2:32080860-32080879 MS.gene065883:CDS 35.0%
! TTGTGATACCTAGGATGTTT+TGG - chr4.2:32079334-32079353 None:intergenic 35.0%
!! AATTTCTGGTATACCTGCTA+TGG + chr4.2:32080204-32080223 MS.gene065883:CDS 35.0%
!! GTGAAATTGATTTGCTGAGA+TGG - chr4.2:32079865-32079884 None:intergenic 35.0%
!! TGCAGTGATTTTGTTGGTTA+AGG - chr4.2:32079933-32079952 None:intergenic 35.0%
!! TTTGGTGATGATGATGTTGT+TGG - chr4.2:32079316-32079335 None:intergenic 35.0%
!!! TGCTGCTTTTTTTGAGAGTT+TGG + chr4.2:32080564-32080583 MS.gene065883:CDS 35.0%
AGCTCAATAGGCGTAAGAAT+CGG - chr4.2:32080322-32080341 None:intergenic 40.0%
ATATGGGCATGCATCGTTTA+AGG - chr4.2:32080023-32080042 None:intergenic 40.0%
CATCATCACCAAAACATCCT+AGG + chr4.2:32079323-32079342 MS.gene065883:CDS 40.0%
CTTGTTGAGCTATTGCCATT+AGG - chr4.2:32079247-32079266 None:intergenic 40.0%
GAGTGAAGCAATTTCGGTTA+GGG - chr4.2:32079801-32079820 None:intergenic 40.0%
GTAGCTGAAATCGATGAAAC+TGG - chr4.2:32080244-32080263 None:intergenic 40.0%
TGAGTGAAGCAATTTCGGTT+AGG - chr4.2:32079802-32079821 None:intergenic 40.0%
TGGTGAAGGAAAGTTGAAAG+CGG - chr4.2:32079504-32079523 None:intergenic 40.0%
TTGAAATGGGTCGGTGAAAT+CGG - chr4.2:32079453-32079472 None:intergenic 40.0%
TTGCTTGGTAGGAGAATTGA+TGG + chr4.2:32080637-32080656 MS.gene065883:CDS 40.0%
! ATGAAGCTAGTTTGACGACT+CGG + chr4.2:32080500-32080519 MS.gene065883:CDS 40.0%
! TAAGGTATCCATCCAGTCAT+CGG - chr4.2:32079570-32079589 None:intergenic 40.0%
! TGAAAACGACGATGTCGTTT+CGG - chr4.2:32079753-32079772 None:intergenic 40.0%
! TTGTTGTTGTTGTTGTGCCA+TGG - chr4.2:32079380-32079399 None:intergenic 40.0%
!! AAACGGGTTGAGATGGAAAT+CGG - chr4.2:32079615-32079634 None:intergenic 40.0%
!! AAGGTTTTGATGGAAAGCTG+TGG + chr4.2:32080703-32080722 MS.gene065883:CDS 40.0%
!! AGAACAGTGGAAGGTTTTGA+TGG + chr4.2:32080693-32080712 MS.gene065883:CDS 40.0%
!! AGGAGAATTGATGGTGTGAT+TGG + chr4.2:32080646-32080665 MS.gene065883:CDS 40.0%
!! ATGATGAAAACGGGTTGAGA+TGG - chr4.2:32079622-32079641 None:intergenic 40.0%
!! GTTTCATCGATTTCAGCTAC+CGG + chr4.2:32080244-32080263 MS.gene065883:CDS 40.0%
!! TGATTTGCTGAGATGGGTTA+GGG - chr4.2:32079858-32079877 None:intergenic 40.0%
!! TGTTGAAAAAGCGCTTCGTT+TGG + chr4.2:32080438-32080457 MS.gene065883:CDS 40.0%
!! TTGATTTGCTGAGATGGGTT+AGG - chr4.2:32079859-32079878 None:intergenic 40.0%
!!! AAGCTGTGGTTTTGAATCAG+TGG + chr4.2:32080717-32080736 MS.gene065883:CDS 40.0%
!!! AGCTGTGGTTTTGAATCAGT+GGG + chr4.2:32080718-32080737 MS.gene065883:CDS 40.0%
!!! CGGTTAGGGTTTTAAGAAGT+GGG - chr4.2:32079787-32079806 None:intergenic 40.0%
!!! GGTTTTAAGAAGTGGGTTTG+TGG - chr4.2:32079780-32079799 None:intergenic 40.0%
!!! TCAACCTGCTTTTTTGTCTC+TGG + chr4.2:32080822-32080841 MS.gene065883:CDS 40.0%
!!! TCGGTTAGGGTTTTAAGAAG+TGG - chr4.2:32079788-32079807 None:intergenic 40.0%
!!! TTGGTGTCGTTTTTTCGTTG+CGG - chr4.2:32079725-32079744 None:intergenic 40.0%
AGAGAGGGTTTGTGATACCT+AGG - chr4.2:32079343-32079362 None:intergenic 45.0%
AGGAAAGTTGAAAGCGGGAT+CGG - chr4.2:32079498-32079517 None:intergenic 45.0%
ATGGAAATCGGAGTGGTTGT+CGG - chr4.2:32079603-32079622 None:intergenic 45.0%
CAAGCCAGAGACAAAAAAGC+AGG - chr4.2:32080829-32080848 None:intergenic 45.0%
CACTGACAGTGGAAACCTAA+TGG + chr4.2:32079229-32079248 MS.gene065883:CDS 45.0%
GAAGTTCCTAAAGCCATAGC+AGG - chr4.2:32080220-32080239 None:intergenic 45.0%
GATGTAGTGAGACAGAGAGA+GGG - chr4.2:32079358-32079377 None:intergenic 45.0%
GCATTTGCAACTCGTTCTTC+TGG + chr4.2:32080163-32080182 MS.gene065883:CDS 45.0%
GCGATTCTTGAAGCAACAGA+AGG + chr4.2:32080073-32080092 MS.gene065883:CDS 45.0%
GGAGGATAAAGAACAGTGGA+AGG + chr4.2:32080684-32080703 MS.gene065883:CDS 45.0%
GGATGGAGGATAAAGAACAG+TGG + chr4.2:32080680-32080699 MS.gene065883:CDS 45.0%
GGTATACCTGCTATGGCTTT+AGG + chr4.2:32080211-32080230 MS.gene065883:CDS 45.0%
GGTGAAGGAAAGTTGAAAGC+GGG - chr4.2:32079503-32079522 None:intergenic 45.0%
GTAGGTGGAAAAGACAAAGG+TGG - chr4.2:32079431-32079450 None:intergenic 45.0%
TCGGTAGGTGGAAAAGACAA+AGG - chr4.2:32079434-32079453 None:intergenic 45.0%
TCTCTGTCTCACTACATCCA+TGG + chr4.2:32079360-32079379 MS.gene065883:CDS 45.0%
! CAAGTGGAGAGTCTTTTGCT+TGG + chr4.2:32080622-32080641 MS.gene065883:CDS 45.0%
! TGAAGCTAGTTTGACGACTC+GGG + chr4.2:32080501-32080520 MS.gene065883:CDS 45.0%
! TGATGGTGTGATTGGTGTGA+GGG + chr4.2:32080654-32080673 MS.gene065883:CDS 45.0%
! TGGAGAGTCTTTTGCTTGGT+AGG + chr4.2:32080626-32080645 MS.gene065883:CDS 45.0%
! TTCACCGACCCATTTCAAGT+CGG + chr4.2:32079455-32079474 MS.gene065883:CDS 45.0%
!! TTGATGGTGTGATTGGTGTG+AGG + chr4.2:32080653-32080672 MS.gene065883:CDS 45.0%
!!! CTGCTTTTTTGTCTCTGGCT+TGG + chr4.2:32080827-32080846 MS.gene065883:CDS 45.0%
!!! TCGTTTTTTCGTTGCGGAGT+GGG - chr4.2:32079719-32079738 None:intergenic 45.0%
AAACGGAAGGTGGTTGAGTG+AGG - chr4.2:32079533-32079552 None:intergenic 50.0%
AACGGAAGGTGGTTGAGTGA+GGG - chr4.2:32079532-32079551 None:intergenic 50.0%
AATGGGTCGGTGAAATCGGT+AGG - chr4.2:32079449-32079468 None:intergenic 50.0%
CCAGTCATCGGAGTCGAAAT+CGG - chr4.2:32079558-32079577 None:intergenic 50.0%
CGGAGTCGAAATCGGAGAAA+CGG - chr4.2:32079550-32079569 None:intergenic 50.0%
CTCTCCACTTGAAACCTCTC+TGG - chr4.2:32080613-32080632 None:intergenic 50.0%
GAAATCGGAGAAACGGAAGG+TGG - chr4.2:32079543-32079562 None:intergenic 50.0%
GGATGTAGTGAGACAGAGAG+AGG - chr4.2:32079359-32079378 None:intergenic 50.0%
GGTTGAGATGGAAATCGGAG+TGG - chr4.2:32079610-32079629 None:intergenic 50.0%
GTCGAAATCGGAGAAACGGA+AGG - chr4.2:32079546-32079565 None:intergenic 50.0%
TTCGCCAGAGAGGTTTCAAG+TGG + chr4.2:32080606-32080625 MS.gene065883:CDS 50.0%
TTTCGACTCCGATGACTGGA+TGG + chr4.2:32079559-32079578 MS.gene065883:CDS 50.0%
! AAACGACGATGTCGTTTCGG+TGG - chr4.2:32079750-32079769 None:intergenic 50.0%
! GATTGGGATTTGGAGTGGCA+CGG - chr4.2:32079690-32079709 None:intergenic 50.0%
! GCTTACATGTGCACTGACAG+TGG + chr4.2:32079218-32079237 MS.gene065883:CDS 50.0%
! GGTTGGATTGGGATTTGGAG+TGG - chr4.2:32079695-32079714 None:intergenic 50.0%
! TCACCGACCCATTTCAAGTC+GGG + chr4.2:32079456-32079475 MS.gene065883:CDS 50.0%
! TGAGAGTTTGGAGCCGAACA+TGG + chr4.2:32080576-32080595 MS.gene065883:CDS 50.0%
! TGGGTGGTTGGATTGGGATT+TGG - chr4.2:32079700-32079719 None:intergenic 50.0%
!! AAAGCGGGATCGGTGTTTTC+AGG - chr4.2:32079488-32079507 None:intergenic 50.0%
!!! GTCGTTTTTTCGTTGCGGAG+TGG - chr4.2:32079720-32079739 None:intergenic 50.0%
!!! TTTTTTCGTTGCGGAGTGGG+TGG - chr4.2:32079716-32079735 None:intergenic 50.0%
CCGATTTCGACTCCGATGAC+TGG + chr4.2:32079555-32079574 MS.gene065883:CDS 55.0%
CGGGTCGGGCATGATGAAAA+CGG - chr4.2:32079632-32079651 None:intergenic 55.0%
GAAATCGGAGTGGTTGTCGG+CGG - chr4.2:32079600-32079619 None:intergenic 55.0%
GATTGGTGTGAGGGAGAGGA+TGG + chr4.2:32080663-32080682 MS.gene065883:CDS 55.0%
GGCGAATCCAACGCCATGTT+CGG - chr4.2:32080592-32080611 None:intergenic 55.0%
GGGTCGGGCATGATGAAAAC+GGG - chr4.2:32079631-32079650 None:intergenic 55.0%
GGTAGGAGAAGATCCGGTTG+CGG - chr4.2:32079662-32079681 None:intergenic 55.0%
GTGTGATTGGTGTGAGGGAG+AGG + chr4.2:32080659-32080678 MS.gene065883:CDS 55.0%
! CGACCCATTTCAAGTCGGGT+CGG + chr4.2:32079460-32079479 MS.gene065883:CDS 55.0%
! CGACCCGACTTGAAATGGGT+CGG - chr4.2:32079462-32079481 None:intergenic 55.0%
! CGATGTCGTTTCGGTGGTGT+TGG - chr4.2:32079744-32079763 None:intergenic 55.0%
! GACCCATTTCAAGTCGGGTC+GGG + chr4.2:32079461-32079480 MS.gene065883:CDS 55.0%
! GACCCGACCCGACTTGAAAT+GGG - chr4.2:32079466-32079485 None:intergenic 55.0%
! GCTAGTTTGACGACTCGGGT+TGG + chr4.2:32080505-32080524 MS.gene065883:CDS 55.0%
! TTTGGAGCCGAACATGGCGT+TGG + chr4.2:32080582-32080601 MS.gene065883:CDS 55.0%
ATCCGGTTGCGGCGAAAGTC+GGG - chr4.2:32079651-32079670 None:intergenic 60.0%
GAGTGAGGGTCGAGTGGTGA+AGG - chr4.2:32079518-32079537 None:intergenic 60.0%
GATCCGGTTGCGGCGAAAGT+CGG - chr4.2:32079652-32079671 None:intergenic 60.0%
GCGGAGTGGGTGGTTGGATT+GGG - chr4.2:32079706-32079725 None:intergenic 60.0%
GGGTCGGTGAAATCGGTAGG+TGG - chr4.2:32079446-32079465 None:intergenic 60.0%
GTGGTTGAGTGAGGGTCGAG+TGG - chr4.2:32079524-32079543 None:intergenic 60.0%
GTTGTCGGCGGCGGACATTA+AGG - chr4.2:32079588-32079607 None:intergenic 60.0%
TGCGGAGTGGGTGGTTGGAT+TGG - chr4.2:32079707-32079726 None:intergenic 60.0%
TGGTGTGAGGGAGAGGATGG+AGG + chr4.2:32080666-32080685 MS.gene065883:CDS 60.0%
TTCGTTGCGGAGTGGGTGGT+TGG - chr4.2:32079712-32079731 None:intergenic 60.0%
! ATTTGGAGTGGCACGGTGGC+GGG - chr4.2:32079683-32079702 None:intergenic 60.0%
! GATTTGGAGTGGCACGGTGG+CGG - chr4.2:32079684-32079703 None:intergenic 60.0%
! GGACCCGACCCGACTTGAAA+TGG - chr4.2:32079467-32079486 None:intergenic 60.0%
! TGGCGTTGGATTCGCCAGAG+AGG + chr4.2:32080596-32080615 MS.gene065883:CDS 60.0%
! TGGGATTTGGAGTGGCACGG+TGG - chr4.2:32079687-32079706 None:intergenic 60.0%
!! GTGGCGGGTAGGAGAAGATC+CGG - chr4.2:32079668-32079687 None:intergenic 60.0%
ATCGGAGTGGTTGTCGGCGG+CGG - chr4.2:32079597-32079616 None:intergenic 65.0%
GACCCGACTTTCGCCGCAAC+CGG + chr4.2:32079646-32079665 MS.gene065883:CDS 65.0%
GGTTGCGGCGAAAGTCGGGT+CGG - chr4.2:32079647-32079666 None:intergenic 65.0%
GTTGCGGCGAAAGTCGGGTC+GGG - chr4.2:32079646-32079665 None:intergenic 65.0%
!! GGAGTGGCACGGTGGCGGGT+AGG - chr4.2:32079679-32079698 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 32079215 32080888 32079215 ID=MS.gene065883
chr4.2 mRNA 32079215 32080888 32079215 ID=MS.gene065883.t1;Parent=MS.gene065883
chr4.2 exon 32079215 32080888 32079215 ID=MS.gene065883.t1.exon1;Parent=MS.gene065883.t1
chr4.2 CDS 32079215 32080888 32079215 ID=cds.MS.gene065883.t1;Parent=MS.gene065883.t1
Gene Sequence

>MS.gene065883

ATGGCTTACATGTGCACTGACAGTGGAAACCTAATGGCAATAGCTCAACAAGTCATCAACCAAAAACAACAACAAGAACAACATGAACAACAACAACACCAACAACATCATCATCACCAAAACATCCTAGGTATCACAAACCCTCTCTCTGTCTCACTACATCCATGGCACAACAACAACAACAACATTCCAGTATCTTCTTCTTCATCACTACCACCTTTGTCTTTTCCACCTACCGATTTCACCGACCCATTTCAAGTCGGGTCGGGTCCTGAAAACACCGATCCCGCTTTCAACTTTCCTTCACCACTCGACCCTCACTCAACCACCTTCCGTTTCTCCGATTTCGACTCCGATGACTGGATGGATACCTTAATGTCCGCCGCCGACAACCACTCCGATTTCCATCTCAACCCGTTTTCATCATGCCCGACCCGACTTTCGCCGCAACCGGATCTTCTCCTACCCGCCACCGTGCCACTCCAAATCCCAATCCAACCACCCACTCCGCAACGAAAAAACGACACCAACACCACCGAAACGACATCGTCGTTTTCATCTTCCACAAACCCACTTCTTAAAACCCTAACCGAAATTGCTTCACTCATCGAAACCCAAAAACCAAATCAAGCAATTGAAACCCTAACCCATCTCAGCAAATCAATTTCACAAAACGGAAACCCTAATCAAAGAGTTTCCTTTTACTTTTCACAAGCCTTAACCAACAAAATCACTGCACAATCTTCAATAGCTTCTTCAAATTCTTCTTCTACTACATGGGAAGAGTTAACACTCTCTTACAAAGCCTTAAACGATGCATGCCCATATTCAAAGTTCGCACATTTAACAGCAAATCAAGCGATTCTTGAAGCAACAGAAGGATCCAACGATATTCATATAGTTGATTTTGGAATTGTTCAAGGGATTCAATGGGCTGCATTGTTACAAGCATTTGCAACTCGTTCTTCTGGTAAACCTAATAGTGTAAGAATTTCTGGTATACCTGCTATGGCTTTAGGAACTTCACCAGTTTCATCGATTTCAGCTACCGGTAATCGTTTATCTGAGTTTGCGAAACTTCTAGAGTTAAATTTTGAGTTTACACCGATTCTTACGCCTATTGAGCTTCTTGATGAATCAAGCTTTTGTATTCAACCAAATGAGGCTTTAGCTGTTAATTTCATGCTTCAGTTGTATAATCTTTTGGATGAGAATATGAATTCTGTTGAAAAAGCGCTTCGTTTGGCGAAATCATTGAATCCTAAGATTGTTACTTTAGGTGAATATGAAGCTAGTTTGACGACTCGGGTTGGATTTGTTGAAAGATTTGAAACTGCTTTTAATTATTTTGCTGCTTTTTTTGAGAGTTTGGAGCCGAACATGGCGTTGGATTCGCCAGAGAGGTTTCAAGTGGAGAGTCTTTTGCTTGGTAGGAGAATTGATGGTGTGATTGGTGTGAGGGAGAGGATGGAGGATAAAGAACAGTGGAAGGTTTTGATGGAAAGCTGTGGTTTTGAATCAGTGGGTTTGAGTCATTATGCAATTAGTCAAGCTAAGATATTGTTGTGGAATTATAGTTATAGTTCTTTGTATTCTCTTGTGGAATCTCAACCTGCTTTTTTGTCTCTGGCTTGGAAAGATGTTCCTCTATTGACTGTTTCTTCTTGGCGTTGA
Protein sequence

>MS.gene065883.t1

MAYMCTDSGNLMAIAQQVINQKQQQEQHEQQQHQQHHHHQNILGITNPLSVSLHPWHNNNNNIPVSSSSSLPPLSFPPTDFTDPFQVGSGPENTDPAFNFPSPLDPHSTTFRFSDFDSDDWMDTLMSAADNHSDFHLNPFSSCPTRLSPQPDLLLPATVPLQIPIQPPTPQRKNDTNTTETTSSFSSSTNPLLKTLTEIASLIETQKPNQAIETLTHLSKSISQNGNPNQRVSFYFSQALTNKITAQSSIASSNSSSTTWEELTLSYKALNDACPYSKFAHLTANQAILEATEGSNDIHIVDFGIVQGIQWAALLQAFATRSSGKPNSVRISGIPAMALGTSPVSSISATGNRLSEFAKLLELNFEFTPILTPIELLDESSFCIQPNEALAVNFMLQLYNLLDENMNSVEKALRLAKSLNPKIVTLGEYEASLTTRVGFVERFETAFNYFAAFFESLEPNMALDSPERFQVESLLLGRRIDGVIGVRERMEDKEQWKVLMESCGFESVGLSHYAISQAKILLWNYSYSSLYSLVESQPAFLSLAWKDVPLLTVSSWR