AlfalfaGEDB | MS.gene065937

M. sativa cultivar XinJiangDaYe / MS.gene065937

KEGG: K20794
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF00583.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065937.t1	XP_024642081.1	90.8	119	9	1	1	119	88	204	1.60E-50	208.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065937.t1	Q568K5	37.0	100	39	2	7	106	88	163	1.2e-06	54.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065937.t1	G7KHW9	90.8	119	9	1	1	119	88	204	1.2e-50	208.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene065937.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065937.t1	MTR_6g072100	90.756	119	9	1	1	119	88	204	6.58e-73	218

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065937.t1	AT1G18335	55.556	108	41	1	1	108	99	199	7.19e-31	111
MS.gene065937.t1	AT1G18335	55.556	108	41	1	1	108	99	199	7.19e-31	111
MS.gene065937.t1	AT1G18335	55.556	108	41	1	1	108	99	199	7.78e-31	111

Find 34 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTACATTACTGCTTTGTTT+TGG	0.323853	6.2:+75288693	MS.gene065937:CDS
CTTAACATGGAAGGGCCATA+TGG	0.380703	6.2:+75288496	None:intergenic
CGTGGCCAGGGGAAAGGATT+GGG	0.393909	6.2:+75288763	MS.gene065937:CDS
TCTTGCCACTCAGCCCCATA+TGG	0.400551	6.2:-75288511	None:intergenic
CACAAATATATAGAGTGCTT+CGG	0.401762	6.2:-75288569	None:intergenic
AAGATAGTGGTCCCTTGGTT+GGG	0.436841	6.2:+75288668	MS.gene065937:CDS
ACAGCTTGAGTCTCGTGGCC+AGG	0.448135	6.2:+75288750	MS.gene065937:CDS
AAAGGTGAAGCGTATAGAAA+TGG	0.455948	6.2:+75288540	MS.gene065937:CDS
AGGATTGGGAAAGCTTCTAA+TGG	0.474839	6.2:+75288777	MS.gene065937:CDS
GAAGATAGTGGTCCCTTGGT+TGG	0.475267	6.2:+75288667	MS.gene065937:CDS
TCTTCTTGACGACTAGTTGA+AGG	0.475356	6.2:-75288649	None:intergenic
TTAACATGGAAGGGCCATAT+GGG	0.490128	6.2:+75288497	None:intergenic
GCTTTCCCAATCCTTTCCCC+TGG	0.495357	6.2:-75288768	None:intergenic
TATTGAGCTTATCGCCCAAA+AGG	0.500241	6.2:+75288804	MS.gene065937:CDS
GAGTCTCGTGGCCAGGGGAA+AGG	0.501909	6.2:+75288757	MS.gene065937:CDS
TCGTGGCCAGGGGAAAGGAT+TGG	0.504530	6.2:+75288762	MS.gene065937:CDS
ACAAATATATAGAGTGCTTC+GGG	0.510555	6.2:-75288568	None:intergenic
ATCTACAGCTTAACATGGAA+GGG	0.529467	6.2:+75288488	None:intergenic
CAGCTTGAGTCTCGTGGCCA+GGG	0.534061	6.2:+75288751	MS.gene065937:CDS
AATCTACAGCTTAACATGGA+AGG	0.544882	6.2:+75288487	None:intergenic
TGAGTGGCAAGAGGAAGAAA+AGG	0.556815	6.2:+75288522	MS.gene065937:CDS
TCAAGAAGATAGTGGTCCCT+TGG	0.557490	6.2:+75288663	MS.gene065937:CDS
CTTCTTGACGACTAGTTGAA+GGG	0.589404	6.2:-75288648	None:intergenic
ACTAGTCGTCAAGAAGATAG+TGG	0.589965	6.2:+75288655	MS.gene065937:CDS
AGTTCATACACATAAAGTAC+CGG	0.630714	6.2:-75288727	None:intergenic
AGCTTGAGTCTCGTGGCCAG+GGG	0.632554	6.2:+75288752	MS.gene065937:CDS
AAGGGCCATATGGGGCTGAG+TGG	0.635636	6.2:+75288506	MS.gene065937:CDS
ATATGGGGCTGAGTGGCAAG+AGG	0.653218	6.2:+75288513	MS.gene065937:CDS
TAACATGGAAGGGCCATATG+GGG	0.654689	6.2:+75288498	None:intergenic
ACTCTATATATTTGTGCACG+AGG	0.656432	6.2:+75288576	MS.gene065937:CDS
GTAATGTACAAACCCAACCA+AGG	0.656995	6.2:-75288680	None:intergenic
GAACTACAGCTTGAGTCTCG+TGG	0.665967	6.2:+75288745	MS.gene065937:CDS
TAATGTACAAACCCAACCAA+GGG	0.673224	6.2:-75288679	None:intergenic
GAAGACAACATTGTTCACTG+AGG	0.772166	6.2:+75288621	MS.gene065937:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGTTCATACACATAAAGTAC+CGG	-	chr6.2:75288730-75288749	None:intergenic	30.0%
!	AAAAACACTTAAGCACCTTT+TGG	-	chr6.2:75288822-75288841	None:intergenic	30.0%
!	AAAACACTTAAGCACCTTTT+GGG	-	chr6.2:75288821-75288840	None:intergenic	30.0%
!	ACAAATATATAGAGTGCTTC+GGG	-	chr6.2:75288571-75288590	None:intergenic	30.0%
!	CACAAATATATAGAGTGCTT+CGG	-	chr6.2:75288572-75288591	None:intergenic	30.0%
!!!	TGTACATTACTGCTTTGTTT+TGG	+	chr6.2:75288693-75288712	MS.gene065937:CDS	30.0%
	AAAGGTGAAGCGTATAGAAA+TGG	+	chr6.2:75288540-75288559	MS.gene065937:CDS	35.0%
	ACTCTATATATTTGTGCACG+AGG	+	chr6.2:75288576-75288595	MS.gene065937:CDS	35.0%
	TAATGTACAAACCCAACCAA+GGG	-	chr6.2:75288682-75288701	None:intergenic	35.0%
	ACTAGTCGTCAAGAAGATAG+TGG	+	chr6.2:75288655-75288674	MS.gene065937:CDS	40.0%
	AGGATTGGGAAAGCTTCTAA+TGG	+	chr6.2:75288777-75288796	MS.gene065937:CDS	40.0%
	GAAGACAACATTGTTCACTG+AGG	+	chr6.2:75288621-75288640	MS.gene065937:CDS	40.0%
	GTAATGTACAAACCCAACCA+AGG	-	chr6.2:75288683-75288702	None:intergenic	40.0%
	TATTGAGCTTATCGCCCAAA+AGG	+	chr6.2:75288804-75288823	MS.gene065937:CDS	40.0%
!	CTTCTTGACGACTAGTTGAA+GGG	-	chr6.2:75288651-75288670	None:intergenic	40.0%
!	TCTTCTTGACGACTAGTTGA+AGG	-	chr6.2:75288652-75288671	None:intergenic	40.0%
!!!	CTGCTTTGTTTTGGAAGAAG+AGG	+	chr6.2:75288702-75288721	MS.gene065937:CDS	40.0%
!!!	TGTTTTGGAAGAAGAGGTAC+CGG	+	chr6.2:75288708-75288727	MS.gene065937:CDS	40.0%
	TGAGTGGCAAGAGGAAGAAA+AGG	+	chr6.2:75288522-75288541	MS.gene065937:CDS	45.0%
!	AAGATAGTGGTCCCTTGGTT+GGG	+	chr6.2:75288668-75288687	MS.gene065937:CDS	45.0%
!	TCAAGAAGATAGTGGTCCCT+TGG	+	chr6.2:75288663-75288682	MS.gene065937:CDS	45.0%
	GAACTACAGCTTGAGTCTCG+TGG	+	chr6.2:75288745-75288764	MS.gene065937:CDS	50.0%
!	GAAGATAGTGGTCCCTTGGT+TGG	+	chr6.2:75288667-75288686	MS.gene065937:CDS	50.0%
	ATATGGGGCTGAGTGGCAAG+AGG	+	chr6.2:75288513-75288532	MS.gene065937:CDS	55.0%
	GCTTTCCCAATCCTTTCCCC+TGG	-	chr6.2:75288771-75288790	None:intergenic	55.0%
	TCTTGCCACTCAGCCCCATA+TGG	-	chr6.2:75288514-75288533	None:intergenic	55.0%
	AAGGGCCATATGGGGCTGAG+TGG	+	chr6.2:75288506-75288525	MS.gene065937:CDS	60.0%
	ACAGCTTGAGTCTCGTGGCC+AGG	+	chr6.2:75288750-75288769	MS.gene065937:CDS	60.0%
	AGCTTGAGTCTCGTGGCCAG+GGG	+	chr6.2:75288752-75288771	MS.gene065937:CDS	60.0%
	CAGCTTGAGTCTCGTGGCCA+GGG	+	chr6.2:75288751-75288770	MS.gene065937:CDS	60.0%
	CGTGGCCAGGGGAAAGGATT+GGG	+	chr6.2:75288763-75288782	MS.gene065937:CDS	60.0%
	TCGTGGCCAGGGGAAAGGAT+TGG	+	chr6.2:75288762-75288781	MS.gene065937:CDS	60.0%
	GAGTCTCGTGGCCAGGGGAA+AGG	+	chr6.2:75288757-75288776	MS.gene065937:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	75288502	75288909	75288502	ID=MS.gene065937
chr6.2	mRNA	75288502	75288909	75288502	ID=MS.gene065937.t1;Parent=MS.gene065937
chr6.2	exon	75288502	75288909	75288502	ID=MS.gene065937.t1.exon1;Parent=MS.gene065937.t1
chr6.2	CDS	75288502	75288909	75288502	ID=cds.MS.gene065937.t1;Parent=MS.gene065937.t1

Gene Sequence

>MS.gene065937

ATGGAAGGGCCATATGGGGCTGAGTGGCAAGAGGAAGAAAAGGTGAAGCGTATAGAAATGGTTGATCCCGAAGCACTCTATATATTTGTGCACGAGGTTAATAATTCAAATGCTGATGAGAAGACAACATTGTTCACTGAGGAAGACCCTTCAACTAGTCGTCAAGAAGATAGTGGTCCCTTGGTTGGGTTTGTACATTACTGCTTTGTTTTGGAAGAAGAGGTACCGGTACTTTATGTGTATGAACTACAGCTTGAGTCTCGTGGCCAGGGGAAAGGATTGGGAAAGCTTCTAATGGAACTTATTGAGCTTATCGCCCAAAAGGTGCTTAAGTGTTTTTCTAAACTTACTCTGACTTTTGATGATAATAGAAAATTATTGCTATGTTGCAAAGAACACAGCTTGTAG

Protein sequence

>MS.gene065937.t1

MEGPYGAEWQEEEKVKRIEMVDPEALYIFVHEVNNSNADEKTTLFTEEDPSTSRQEDSGPLVGFVHYCFVLEEEVPVLYVYELQLESRGQGKGLGKLLMELIELIAQKVLKCFSKLTLTFDDNRKLLLCCKEHSL