AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066049

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066049.t1 MTR_1g075890 81.930 653 53 3 1 588 1 653 0.0 1026
MS.gene066049.t1 MTR_3g464260 71.067 553 77 8 37 588 29 499 0.0 742
MS.gene066049.t1 MTR_3g464280 68.021 566 103 7 1 560 1 494 0.0 699
MS.gene066049.t1 MTR_5g071070 53.481 675 218 15 1 587 1 667 0.0 630
MS.gene066049.t1 MTR_5g071070 53.120 593 184 14 81 587 1 585 0.0 540
MS.gene066049.t1 MTR_5g082830 46.330 654 238 16 19 582 13 643 0.0 528
MS.gene066049.t1 MTR_7g092070 40.582 653 266 16 33 582 47 680 6.50e-143 431
MS.gene066049.t1 MTR_3g070880 44.792 576 193 18 31 579 8 485 3.93e-139 414
MS.gene066049.t1 MTR_3g464290 56.813 433 139 9 177 587 54 460 9.19e-135 401
MS.gene066049.t1 MTR_3g029590 37.688 666 284 18 30 584 42 687 6.54e-128 392
MS.gene066049.t1 MTR_3g464270 54.768 367 139 5 222 578 8 357 4.44e-122 365
MS.gene066049.t1 MTR_1g050438 40.773 466 220 11 33 451 15 471 2.56e-103 324
MS.gene066049.t1 MTR_0728s0020 40.773 466 220 11 33 451 31 487 8.29e-103 324
MS.gene066049.t1 MTR_3g435590 47.091 361 174 5 30 374 29 388 3.58e-102 317
MS.gene066049.t1 MTR_3g435680 41.096 292 136 4 118 378 26 312 2.73e-65 219
MS.gene066049.t1 MTR_8g102100 53.659 164 70 1 219 376 77 240 3.69e-55 191
MS.gene066049.t1 MTR_6g090380 69.159 107 33 0 219 325 99 205 6.33e-53 185
MS.gene066049.t1 MTR_3g103960 54.658 161 61 4 187 344 25 176 2.10e-51 178
MS.gene066049.t1 MTR_2g090605 61.261 111 43 0 223 333 77 187 2.29e-48 174
MS.gene066049.t1 MTR_4g078660 57.944 107 45 0 219 325 56 162 5.95e-47 167
MS.gene066049.t1 MTR_1g081420 75.806 62 8 1 478 532 167 228 5.25e-20 89.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066049.t1 AT5G58620 49.503 604 239 20 21 579 2 584 0.0 526
MS.gene066049.t1 AT3G55980 46.231 597 253 14 1 579 1 547 1.59e-165 484
MS.gene066049.t1 AT3G55980 46.231 597 253 14 1 579 68 614 1.30e-164 484
MS.gene066049.t1 AT2G40140 46.000 600 258 17 1 579 1 555 2.84e-160 471
MS.gene066049.t1 AT2G40140 46.000 600 258 17 1 579 1 555 2.84e-160 471
MS.gene066049.t1 AT5G12850 41.993 612 256 20 25 547 34 635 1.88e-135 411
MS.gene066049.t1 AT2G41900 46.921 471 183 8 25 447 40 491 1.84e-134 409
MS.gene066049.t1 AT4G29190 42.128 235 101 6 212 435 68 278 1.92e-54 189
MS.gene066049.t1 AT5G07500 60.331 121 48 0 205 325 34 154 1.55e-52 180
MS.gene066049.t1 AT2G19810 62.295 122 44 1 208 327 61 182 7.87e-52 182
MS.gene066049.t1 AT2G25900 58.140 129 54 0 219 347 86 214 1.19e-51 180
MS.gene066049.t1 AT2G25900 58.140 129 54 0 219 347 86 214 1.19e-51 180
MS.gene066049.t1 AT1G03790 50.968 155 68 3 190 340 53 203 5.97e-48 172
MS.gene066049.t1 AT5G44260 56.250 128 51 2 201 327 42 165 1.70e-45 165

Find 141 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCTTCATTGTGCTGTTTC+TGG 0.137009 1.2:+58528864 MS.gene066049:CDS
ACCTTCTGCCTTCTCTGGTT+GGG 0.217111 1.2:+58530075 MS.gene066049:CDS
CTTGTCAAACTGGTTTGATT+AGG 0.230886 1.2:+58528550 MS.gene066049:CDS
GGTGTGTTGTCTTATATTCT+TGG 0.275343 1.2:+58528792 MS.gene066049:CDS
GAACGCCATTATATCATCTT+TGG 0.288121 1.2:-58528637 None:intergenic
TTGTCAAACTGGTTTGATTA+GGG 0.297404 1.2:+58528551 MS.gene066049:CDS
GAACTTGAAAATCGCCTTAC+AGG 0.298409 1.2:+58529776 MS.gene066049:CDS
TATATAGTACAGATGAATTT+AGG 0.309340 1.2:+58529177 MS.gene066049:CDS
GACGATTTCCTTAAGAAAAT+AGG 0.314739 1.2:+58529056 MS.gene066049:CDS
CTTGTTGTTGCTCCACCATT+TGG 0.317761 1.2:-58529082 None:intergenic
CTTATGGTTGCTGCTTTGTT+TGG 0.322501 1.2:+58528762 MS.gene066049:CDS
GAGTGCCCCTTTGTTCATCC+TGG 0.343455 1.2:+58529254 MS.gene066049:CDS
GAACGCAAACCAGCAACTTT+GGG 0.343665 1.2:+58529901 MS.gene066049:CDS
GTGTCCCTTGCCCTGAGTTT+CGG 0.346896 1.2:+58529321 MS.gene066049:CDS
AATAGAAAACCTTGTCAAAC+TGG 0.348122 1.2:+58528540 MS.gene066049:CDS
ATGGAACCTTCTGCCTTCTC+TGG 0.354986 1.2:+58530070 MS.gene066049:CDS
TGTTGATCAAATTCCATTGT+TGG 0.356576 1.2:-58530265 None:intergenic
TCTCCTTCAAATAGGCTTGC+AGG 0.358629 1.2:+58529806 MS.gene066049:CDS
AAGGATTGCCCCAAAGTTGC+TGG 0.360358 1.2:-58529910 None:intergenic
TTCAGCCAAAGATGATATAA+TGG 0.367048 1.2:+58528632 MS.gene066049:CDS
AACCTTCTGCCTTCTCTGGT+TGG 0.384069 1.2:+58530074 MS.gene066049:CDS
AGAACGCAAACCAGCAACTT+TGG 0.384644 1.2:+58529900 MS.gene066049:CDS
TTCACCAAGTGCTTCTTCAA+TGG 0.385276 1.2:+58529553 MS.gene066049:CDS
GAAGGTTGGATCAAAAGAAA+TGG 0.391384 1.2:+58528719 MS.gene066049:CDS
TGAAGAGAGGACACCTCTTA+TGG 0.399251 1.2:+58528746 MS.gene066049:CDS
TTGTCTTATATTCTTGGAAC+CGG 0.399584 1.2:+58528798 MS.gene066049:CDS
GTCTTGCATCTCCTTCAAAT+AGG 0.399881 1.2:+58529798 MS.gene066049:CDS
CCGCTGATGCAAACGGTAAC+CGG 0.400035 1.2:+58528949 MS.gene066049:CDS
GTGAATCAACCCAAGATTCA+TGG 0.407246 1.2:-58530228 None:intergenic
TTTGAATTGGTAAGATCAAA+AGG 0.409476 1.2:-58529929 None:intergenic
GCAAACCCTTCTCATGCATC+TGG 0.411034 1.2:-58529447 None:intergenic
TGTTGCGCTCAATAAAGCTT+TGG 0.411592 1.2:-58530000 None:intergenic
CTAAGAGTATCTTCGGTTCA+AGG 0.415813 1.2:+58528997 MS.gene066049:CDS
TCAAAGTGAAGCCTTGTTCA+AGG 0.417464 1.2:+58529210 MS.gene066049:CDS
CCTTCTGCCTTCTCTGGTTG+GGG 0.417819 1.2:+58530076 MS.gene066049:CDS
TGCTGCTTTGTTTGGAAGCA+AGG 0.432478 1.2:+58528770 MS.gene066049:CDS
TCTGGTTGGGGCTCTCCTGA+TGG 0.432980 1.2:+58530088 MS.gene066049:CDS
CAAGGACTTATACTCATGAT+TGG 0.436359 1.2:+58529228 MS.gene066049:CDS
TGATGCAATCACATTTGAAT+TGG 0.437985 1.2:-58529942 None:intergenic
TATATTCCATTCTTTATGTC+AGG 0.439563 1.2:-58529158 None:intergenic
GTCAGGTAGGGATGGATCAA+TGG 0.440308 1.2:-58529141 None:intergenic
AGTGCCCCTTTGTTCATCCT+GGG 0.442388 1.2:+58529255 MS.gene066049:CDS
AACAAAGCAGCAACCATAAG+AGG 0.442942 1.2:-58528759 None:intergenic
ACCGAGCGTGCGGCTCAGAT+AGG 0.449079 1.2:+58528835 MS.gene066049:CDS
ATTGAGTTCATTGCCGGGGA+TGG 0.450856 1.2:-58530125 None:intergenic
TTCTGCTAGCTCAGTTGCCA+TGG 0.451503 1.2:+58530051 MS.gene066049:CDS
AAGGTTGGATCAAAAGAAAT+GGG 0.451915 1.2:+58528720 MS.gene066049:CDS
TCGGTTTCTAAGAGTATCTT+CGG 0.453417 1.2:+58528990 MS.gene066049:CDS
GGAAGGGATCATGCAACAAA+GGG 0.458959 1.2:+58529342 MS.gene066049:CDS
ACTCCTGCAAGCCTATTTGA+AGG 0.462316 1.2:-58529809 None:intergenic
TTATGGTATGGAAGGAAGGT+TGG 0.462581 1.2:+58528705 MS.gene066049:CDS
CATGATCCCTTCCGAAACTC+AGG 0.476678 1.2:-58529332 None:intergenic
CCCTATCTGAGCCGCACGCT+CGG 0.476697 1.2:-58528836 None:intergenic
GTTGATTCACTTGTGAATGA+TGG 0.479955 1.2:+58530241 MS.gene066049:CDS
TCCATTCTTTATGTCAGGTA+GGG 0.481887 1.2:-58529153 None:intergenic
TTCTTTATGTCAGGTAGGGA+TGG 0.483088 1.2:-58529149 None:intergenic
TACAACGGTCGAAGCTCCTC+TGG 0.485266 1.2:-58529485 None:intergenic
GCAGCATCATCGCCTACGGC+TGG 0.487982 1.2:+58529644 MS.gene066049:CDS
CGACCGTTGTATGCTTCTAC+CGG 0.490079 1.2:+58529497 MS.gene066049:CDS
GAAAATAGGTTTCCAAATGG+TGG 0.491205 1.2:+58529070 MS.gene066049:CDS
CTTCTCGGCATCTGAAGGGT+AGG 0.494265 1.2:-58529707 None:intergenic
ATCTGCTTCTCGGCATCTGA+AGG 0.497474 1.2:-58529712 None:intergenic
AGCCTTGTTCTGTATTGAGC+AGG 0.498591 1.2:-58529410 None:intergenic
GGGTGAAGGTAGAGCAGAAC+CGG 0.500946 1.2:-58529516 None:intergenic
CCCTTGCCCTGAGTTTCGGA+AGG 0.503997 1.2:+58529325 MS.gene066049:CDS
AGGGTTATGGTATGGAAGGA+AGG 0.505824 1.2:+58528701 MS.gene066049:CDS
TCCCTACCTGACATAAAGAA+TGG 0.512480 1.2:+58529152 MS.gene066049:CDS
TAAGAAAATAGGTTTCCAAA+TGG 0.517234 1.2:+58529067 MS.gene066049:CDS
GCTTTGGCTCCGCTTCGAAA+AGG 0.520561 1.2:-58529984 None:intergenic
TCAGGTAGGGATGGATCAAT+GGG 0.521506 1.2:-58529140 None:intergenic
AAGTAGGGTTATGGTATGGA+AGG 0.522426 1.2:+58528697 MS.gene066049:CDS
GTGCAGTGACTTGATTGTCT+CGG 0.522663 1.2:+58528971 MS.gene066049:CDS
CCCTTCCGAAACTCAGGGCA+AGG 0.525679 1.2:-58529326 None:intergenic
GATCATGATGTTGATGAAGT+AGG 0.526063 1.2:+58528681 MS.gene066049:CDS
ATGTTGATGAAGTAGGGTTA+TGG 0.527912 1.2:+58528688 MS.gene066049:CDS
GTATCTTCGGTTCAAGGAAG+AGG 0.528667 1.2:+58529003 MS.gene066049:CDS
CCGGTTACCGTTTGCATCAG+CGG 0.529093 1.2:-58528949 None:intergenic
GCTGCTTTGTTTGGAAGCAA+GGG 0.529208 1.2:+58528771 MS.gene066049:CDS
ATCATGATGTTGATGAAGTA+GGG 0.530587 1.2:+58528682 MS.gene066049:CDS
AAGGAGATGCAAGACCTGTA+AGG 0.541933 1.2:-58529790 None:intergenic
AGGAGAGCCCCAACCAGAGA+AGG 0.547335 1.2:-58530083 None:intergenic
CCGAGCGTGCGGCTCAGATA+GGG 0.547437 1.2:+58528836 MS.gene066049:CDS
CTCCTGATGGAAAATTAGAC+TGG 0.548612 1.2:+58530101 MS.gene066049:CDS
CGGAAGGGATCATGCAACAA+AGG 0.550435 1.2:+58529341 MS.gene066049:CDS
CAGAAGTAGCACTTCAACAA+TGG 0.550435 1.2:+58530165 MS.gene066049:CDS
TTCTCGGCATCTGAAGGGTA+GGG 0.550744 1.2:-58529706 None:intergenic
TTCATCCTGGGGAAAATGCA+AGG 0.557730 1.2:+58529267 MS.gene066049:CDS
GTTAGTTCCGCTGATGCAAA+CGG 0.558347 1.2:+58528942 MS.gene066049:CDS
TCGGTTAACATCAACGCGGC+CGG 0.559271 1.2:-58528817 None:intergenic
TTGATTAGGGAAAATGAATG+TGG 0.561114 1.2:+58528564 MS.gene066049:CDS
ATCTGCAGCATCATCGCCTA+CGG 0.562339 1.2:+58529640 MS.gene066049:CDS
AAATTAGACTGGTCCATCCC+CGG 0.564430 1.2:+58530112 MS.gene066049:CDS
GGTGAATTTGAATATGATGT+GGG 0.570481 1.2:-58529536 None:intergenic
GCAATGAACTCAATAAAATG+AGG 0.575881 1.2:+58530134 MS.gene066049:CDS
TTCCATTCTTTATGTCAGGT+AGG 0.576271 1.2:-58529154 None:intergenic
GTGCCCCTTTGTTCATCCTG+GGG 0.580017 1.2:+58529256 MS.gene066049:CDS
CATGTGTAATGATATTTCAT+CGG 0.580467 1.2:-58529299 None:intergenic
ATGAAACCAGATGCATGAGA+AGG 0.586951 1.2:+58529441 MS.gene066049:CDS
CTAATCAAACCAGTTTGACA+AGG 0.588552 1.2:-58528549 None:intergenic
AGAGAAGGCAGAAGGTTCCA+TGG 0.589015 1.2:-58530068 None:intergenic
TGAAACCAGATGCATGAGAA+GGG 0.591472 1.2:+58529442 MS.gene066049:CDS
TTGTTCTGCTGCTTCTGCCG+AGG 0.591938 1.2:+58528887 MS.gene066049:CDS
CCCCAACCAGAGAAGGCAGA+AGG 0.594817 1.2:-58530076 None:intergenic
CCTTGCCCTGAGTTTCGGAA+GGG 0.598605 1.2:+58529326 MS.gene066049:CDS
ATACAGAACAAGGCTTTGCA+AGG 0.598747 1.2:+58529418 MS.gene066049:CDS
GTAGGCGATGATGCTGCAGA+TGG 0.600318 1.2:-58529638 None:intergenic
TGCCACATGGTTCCAGCCGT+AGG 0.601454 1.2:-58529656 None:intergenic
TGATGCACTTGTATTGATGT+CGG 0.604850 1.2:-58529878 None:intergenic
GATGAAGTAGGGTTATGGTA+TGG 0.604921 1.2:+58528693 MS.gene066049:CDS
GAATCCATTGAAGAAGCACT+TGG 0.606204 1.2:-58529557 None:intergenic
GTGGAGCAACAACAAGATGT+AGG 0.606526 1.2:+58529089 MS.gene066049:CDS
TTGGCTGAAAACTCAAGCAA+AGG 0.608890 1.2:-58528618 None:intergenic
TGGTGAATTTGAATATGATG+TGG 0.620600 1.2:-58529537 None:intergenic
TCTGCTTCTCGGCATCTGAA+GGG 0.621299 1.2:-58529711 None:intergenic
ATGGCAACTGAGCTAGCAGA+AGG 0.621567 1.2:-58530049 None:intergenic
AAGAAATGGGATATGAAGAG+AGG 0.624986 1.2:+58528733 MS.gene066049:CDS
AACGCAAACCAGCAACTTTG+GGG 0.627454 1.2:+58529902 MS.gene066049:CDS
AAAACAAAAGTCATGTGCAA+CGG 0.630659 1.2:+58528513 None:intergenic
AAGCAAAAGTTTGACAACCT+CGG 0.631058 1.2:-58528904 None:intergenic
TGAGTATAAGTCCTTGAACA+AGG 0.635355 1.2:-58529221 None:intergenic
ATCCTGCTCAATACAGAACA+AGG 0.635511 1.2:+58529408 MS.gene066049:CDS
AATGATATTTCATCGGATCA+CGG 0.635978 1.2:-58529292 None:intergenic
CTGAATCTGAGTCTGCCACA+TGG 0.636502 1.2:-58529669 None:intergenic
TTTGAATATGATGTGGGTGA+AGG 0.636666 1.2:-58529530 None:intergenic
TGTGAATGATGGTCCAACAA+TGG 0.642483 1.2:+58530252 MS.gene066049:CDS
GCAGATTGTATCAAAGAAGA+CGG 0.643659 1.2:-58529596 None:intergenic
CCTTCCGAAACTCAGGGCAA+GGG 0.646387 1.2:-58529325 None:intergenic
AGACAATCAAGTCACTGCAC+CGG 0.647436 1.2:-58528968 None:intergenic
GTTGATGTTAACCGAGCGTG+CGG 0.649710 1.2:+58528825 MS.gene066049:CDS
GATGCTGCAGATGGAGTCAA+AGG 0.650089 1.2:-58529629 None:intergenic
ATGATCCCTTCCGAAACTCA+GGG 0.652947 1.2:-58529331 None:intergenic
TCAAGGAAGAGGATACTACA+AGG 0.674650 1.2:+58529014 MS.gene066049:CDS
ACAAGGCAATGTTGATGACG+AGG 0.685237 1.2:+58529031 MS.gene066049:CDS
CGCCTACGGCTGGAACCATG+TGG 0.694097 1.2:+58529654 MS.gene066049:CDS
GCTGCAGATGGAGTCAAAGG+TGG 0.696337 1.2:-58529626 None:intergenic
TTTCCCCAGGATGAACAAAG+GGG 0.706386 1.2:-58529259 None:intergenic
GAAGGGATCATGCAACAAAG+GGG 0.715610 1.2:+58529343 MS.gene066049:CDS
AGAAACAGCACAATGAAGAG+CGG 0.722067 1.2:-58528862 None:intergenic
GATGCTTGTGAATATGCACA+TGG 0.754358 1.2:+58529365 MS.gene066049:CDS
GAACCGGTAGAAGCATACAA+CGG 0.780700 1.2:-58529500 None:intergenic
ACGCTCGGTTAACATCAACG+CGG 0.858999 1.2:-58528821 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATAGTACAGATGAATTT+AGG + chr1.2:58529177-58529196 MS.gene066049:CDS 20.0%
! CATGTGTAATGATATTTCAT+CGG - chr1.2:58529302-58529321 None:intergenic 25.0%
! TAAGAAAATAGGTTTCCAAA+TGG + chr1.2:58529067-58529086 MS.gene066049:CDS 25.0%
! TATATTCCATTCTTTATGTC+AGG - chr1.2:58529161-58529180 None:intergenic 25.0%
! TCATGGCTTAAAAATATATC+TGG - chr1.2:58530214-58530233 None:intergenic 25.0%
! TTTGAATTGGTAAGATCAAA+AGG - chr1.2:58529932-58529951 None:intergenic 25.0%
!! ACTAACCTTGAAAACATTTT+CGG + chr1.2:58529839-58529858 MS.gene066049:CDS 25.0%
!! TTTGGAAACCTATTTTCTTA+AGG - chr1.2:58529067-58529086 None:intergenic 25.0%
!!! AAAATGTTTTCAAGGTTAGT+AGG - chr1.2:58529839-58529858 None:intergenic 25.0%
!!! ATTTTTAAGCCATGAATCTT+GGG + chr1.2:58530219-58530238 MS.gene066049:CDS 25.0%
!!! TATTTTTAAGCCATGAATCT+TGG + chr1.2:58530218-58530237 MS.gene066049:CDS 25.0%
AAGGTTGGATCAAAAGAAAT+GGG + chr1.2:58528720-58528739 MS.gene066049:CDS 30.0%
AATAGAAAACCTTGTCAAAC+TGG + chr1.2:58528540-58528559 MS.gene066049:CDS 30.0%
AATGATATTTCATCGGATCA+CGG - chr1.2:58529295-58529314 None:intergenic 30.0%
GACGATTTCCTTAAGAAAAT+AGG + chr1.2:58529056-58529075 MS.gene066049:CDS 30.0%
GCAATGAACTCAATAAAATG+AGG + chr1.2:58530134-58530153 MS.gene066049:CDS 30.0%
GGTGAATTTGAATATGATGT+GGG - chr1.2:58529539-58529558 None:intergenic 30.0%
TGATGCAATCACATTTGAAT+TGG - chr1.2:58529945-58529964 None:intergenic 30.0%
TGGTGAATTTGAATATGATG+TGG - chr1.2:58529540-58529559 None:intergenic 30.0%
TGTTGATCAAATTCCATTGT+TGG - chr1.2:58530268-58530287 None:intergenic 30.0%
TTCAGCCAAAGATGATATAA+TGG + chr1.2:58528632-58528651 MS.gene066049:CDS 30.0%
TTGATTAGGGAAAATGAATG+TGG + chr1.2:58528564-58528583 MS.gene066049:CDS 30.0%
TTGTCTTATATTCTTGGAAC+CGG + chr1.2:58528798-58528817 MS.gene066049:CDS 30.0%
! ATCATGATGTTGATGAAGTA+GGG + chr1.2:58528682-58528701 MS.gene066049:CDS 30.0%
! ATTTTATTGAGTTCATTGCC+GGG - chr1.2:58530133-58530152 None:intergenic 30.0%
! CATTTTATTGAGTTCATTGC+CGG - chr1.2:58530134-58530153 None:intergenic 30.0%
! CTAACCTTGAAAACATTTTC+GGG + chr1.2:58529840-58529859 MS.gene066049:CDS 30.0%
!! CACATGGTATTTTTGAATGT+TGG + chr1.2:58529381-58529400 MS.gene066049:CDS 30.0%
!! TTGTCAAACTGGTTTGATTA+GGG + chr1.2:58528551-58528570 MS.gene066049:CDS 30.0%
AAGAAATGGGATATGAAGAG+AGG + chr1.2:58528733-58528752 MS.gene066049:CDS 35.0%
AAGCAAAAGTTTGACAACCT+CGG - chr1.2:58528907-58528926 None:intergenic 35.0%
CAAGGACTTATACTCATGAT+TGG + chr1.2:58529228-58529247 MS.gene066049:CDS 35.0%
CTAATCAAACCAGTTTGACA+AGG - chr1.2:58528552-58528571 None:intergenic 35.0%
GAAAATAGGTTTCCAAATGG+TGG + chr1.2:58529070-58529089 MS.gene066049:CDS 35.0%
GAACGCCATTATATCATCTT+TGG - chr1.2:58528640-58528659 None:intergenic 35.0%
GAAGGTTGGATCAAAAGAAA+TGG + chr1.2:58528719-58528738 MS.gene066049:CDS 35.0%
GCAGATTGTATCAAAGAAGA+CGG - chr1.2:58529599-58529618 None:intergenic 35.0%
GGTGTGTTGTCTTATATTCT+TGG + chr1.2:58528792-58528811 MS.gene066049:CDS 35.0%
TCCATTCTTTATGTCAGGTA+GGG - chr1.2:58529156-58529175 None:intergenic 35.0%
TGAGTATAAGTCCTTGAACA+AGG - chr1.2:58529224-58529243 None:intergenic 35.0%
TTCCATTCTTTATGTCAGGT+AGG - chr1.2:58529157-58529176 None:intergenic 35.0%
TTTGAATATGATGTGGGTGA+AGG - chr1.2:58529533-58529552 None:intergenic 35.0%
! ATGACCCGAAAATGTTTTCA+AGG - chr1.2:58529847-58529866 None:intergenic 35.0%
! ATGTTGATGAAGTAGGGTTA+TGG + chr1.2:58528688-58528707 MS.gene066049:CDS 35.0%
! GATCATGATGTTGATGAAGT+AGG + chr1.2:58528681-58528700 MS.gene066049:CDS 35.0%
! GTTGATTCACTTGTGAATGA+TGG + chr1.2:58530241-58530260 MS.gene066049:CDS 35.0%
! TGATGCACTTGTATTGATGT+CGG - chr1.2:58529881-58529900 None:intergenic 35.0%
! TTTTATTGAGTTCATTGCCG+GGG - chr1.2:58530132-58530151 None:intergenic 35.0%
!! CTTGTCAAACTGGTTTGATT+AGG + chr1.2:58528550-58528569 MS.gene066049:CDS 35.0%
!! TCGGTTTCTAAGAGTATCTT+CGG + chr1.2:58528990-58529009 MS.gene066049:CDS 35.0%
!!! GCAGTTTTAAATCTGCTTCT+CGG - chr1.2:58529725-58529744 None:intergenic 35.0%
AACAAAGCAGCAACCATAAG+AGG - chr1.2:58528762-58528781 None:intergenic 40.0%
AAGTAGGGTTATGGTATGGA+AGG + chr1.2:58528697-58528716 MS.gene066049:CDS 40.0%
AGAAACAGCACAATGAAGAG+CGG - chr1.2:58528865-58528884 None:intergenic 40.0%
ATACAGAACAAGGCTTTGCA+AGG + chr1.2:58529418-58529437 MS.gene066049:CDS 40.0%
ATCCTGCTCAATACAGAACA+AGG + chr1.2:58529408-58529427 MS.gene066049:CDS 40.0%
ATGAAACCAGATGCATGAGA+AGG + chr1.2:58529441-58529460 MS.gene066049:CDS 40.0%
CAGAAGTAGCACTTCAACAA+TGG + chr1.2:58530165-58530184 MS.gene066049:CDS 40.0%
CTCCTGATGGAAAATTAGAC+TGG + chr1.2:58530101-58530120 MS.gene066049:CDS 40.0%
GAACTTGAAAATCGCCTTAC+AGG + chr1.2:58529776-58529795 MS.gene066049:CDS 40.0%
GATGAAGTAGGGTTATGGTA+TGG + chr1.2:58528693-58528712 MS.gene066049:CDS 40.0%
GATGCTTGTGAATATGCACA+TGG + chr1.2:58529365-58529384 MS.gene066049:CDS 40.0%
GTCTTGCATCTCCTTCAAAT+AGG + chr1.2:58529798-58529817 MS.gene066049:CDS 40.0%
GTGAATCAACCCAAGATTCA+TGG - chr1.2:58530231-58530250 None:intergenic 40.0%
TCAAGGAAGAGGATACTACA+AGG + chr1.2:58529014-58529033 MS.gene066049:CDS 40.0%
TCCCTACCTGACATAAAGAA+TGG + chr1.2:58529152-58529171 MS.gene066049:CDS 40.0%
TGAAACCAGATGCATGAGAA+GGG + chr1.2:58529442-58529461 MS.gene066049:CDS 40.0%
TGTGAATGATGGTCCAACAA+TGG + chr1.2:58530252-58530271 MS.gene066049:CDS 40.0%
TGTTGCGCTCAATAAAGCTT+TGG - chr1.2:58530003-58530022 None:intergenic 40.0%
TTATGGTATGGAAGGAAGGT+TGG + chr1.2:58528705-58528724 MS.gene066049:CDS 40.0%
TTCACCAAGTGCTTCTTCAA+TGG + chr1.2:58529553-58529572 MS.gene066049:CDS 40.0%
TTCTTTATGTCAGGTAGGGA+TGG - chr1.2:58529152-58529171 None:intergenic 40.0%
TTGGCTGAAAACTCAAGCAA+AGG - chr1.2:58528621-58528640 None:intergenic 40.0%
! ATTTTCCCCAGGATGAACAA+AGG - chr1.2:58529264-58529283 None:intergenic 40.0%
! GACCAGTCTAATTTTCCATC+AGG - chr1.2:58530106-58530125 None:intergenic 40.0%
! TTTTCCCCAGGATGAACAAA+GGG - chr1.2:58529263-58529282 None:intergenic 40.0%
!! CTAAGAGTATCTTCGGTTCA+AGG + chr1.2:58528997-58529016 MS.gene066049:CDS 40.0%
!! CTTATGGTTGCTGCTTTGTT+TGG + chr1.2:58528762-58528781 MS.gene066049:CDS 40.0%
!! GAATCCATTGAAGAAGCACT+TGG - chr1.2:58529560-58529579 None:intergenic 40.0%
!! TCAAAGTGAAGCCTTGTTCA+AGG + chr1.2:58529210-58529229 MS.gene066049:CDS 40.0%
AACGCAAACCAGCAACTTTG+GGG + chr1.2:58529902-58529921 MS.gene066049:CDS 45.0%
AAGGAGATGCAAGACCTGTA+AGG - chr1.2:58529793-58529812 None:intergenic 45.0%
ACTCCTGCAAGCCTATTTGA+AGG - chr1.2:58529812-58529831 None:intergenic 45.0%
AGAACGCAAACCAGCAACTT+TGG + chr1.2:58529900-58529919 MS.gene066049:CDS 45.0%
AGACAATCAAGTCACTGCAC+CGG - chr1.2:58528971-58528990 None:intergenic 45.0%
AGGGTTATGGTATGGAAGGA+AGG + chr1.2:58528701-58528720 MS.gene066049:CDS 45.0%
ATGATCCCTTCCGAAACTCA+GGG - chr1.2:58529334-58529353 None:intergenic 45.0%
GAACGCAAACCAGCAACTTT+GGG + chr1.2:58529901-58529920 MS.gene066049:CDS 45.0%
GAAGGGATCATGCAACAAAG+GGG + chr1.2:58529343-58529362 MS.gene066049:CDS 45.0%
GGAAGGGATCATGCAACAAA+GGG + chr1.2:58529342-58529361 MS.gene066049:CDS 45.0%
GTATCTTCGGTTCAAGGAAG+AGG + chr1.2:58529003-58529022 MS.gene066049:CDS 45.0%
GTGCAGTGACTTGATTGTCT+CGG + chr1.2:58528971-58528990 MS.gene066049:CDS 45.0%
GTGGAGCAACAACAAGATGT+AGG + chr1.2:58529089-58529108 MS.gene066049:CDS 45.0%
GTTAGTTCCGCTGATGCAAA+CGG + chr1.2:58528942-58528961 MS.gene066049:CDS 45.0%
TCAGGTAGGGATGGATCAAT+GGG - chr1.2:58529143-58529162 None:intergenic 45.0%
TCTCCTTCAAATAGGCTTGC+AGG + chr1.2:58529806-58529825 MS.gene066049:CDS 45.0%
TGAAGAGAGGACACCTCTTA+TGG + chr1.2:58528746-58528765 MS.gene066049:CDS 45.0%
TTCATCCTGGGGAAAATGCA+AGG + chr1.2:58529267-58529286 MS.gene066049:CDS 45.0%
TTTCCCCAGGATGAACAAAG+GGG - chr1.2:58529262-58529281 None:intergenic 45.0%
! AAATTAGACTGGTCCATCCC+CGG + chr1.2:58530112-58530131 MS.gene066049:CDS 45.0%
! GAACCGGTAGAAGCATACAA+CGG - chr1.2:58529503-58529522 None:intergenic 45.0%
!! ACAAGGCAATGTTGATGACG+AGG + chr1.2:58529031-58529050 MS.gene066049:CDS 45.0%
!! AGCCTTGTTCTGTATTGAGC+AGG - chr1.2:58529413-58529432 None:intergenic 45.0%
!! CTTGTTGTTGCTCCACCATT+TGG - chr1.2:58529085-58529104 None:intergenic 45.0%
!! CTTTTTCGCTCACAAACCAG+AGG + chr1.2:58529469-58529488 MS.gene066049:CDS 45.0%
!! GCTCTTCATTGTGCTGTTTC+TGG + chr1.2:58528864-58528883 MS.gene066049:CDS 45.0%
!! GCTGCTTTGTTTGGAAGCAA+GGG + chr1.2:58528771-58528790 MS.gene066049:CDS 45.0%
!! TGCTGCTTTGTTTGGAAGCA+AGG + chr1.2:58528770-58528789 MS.gene066049:CDS 45.0%
!!! GAGTTGATGCCTTTTCGAAG+CGG + chr1.2:58529975-58529994 MS.gene066049:CDS 45.0%
AACCTTCTGCCTTCTCTGGT+TGG + chr1.2:58530074-58530093 MS.gene066049:CDS 50.0%
AAGGATTGCCCCAAAGTTGC+TGG - chr1.2:58529913-58529932 None:intergenic 50.0%
ACCTTCTGCCTTCTCTGGTT+GGG + chr1.2:58530075-58530094 MS.gene066049:CDS 50.0%
ACGCTCGGTTAACATCAACG+CGG - chr1.2:58528824-58528843 None:intergenic 50.0%
AGAGAAGGCAGAAGGTTCCA+TGG - chr1.2:58530071-58530090 None:intergenic 50.0%
AGTGCCCCTTTGTTCATCCT+GGG + chr1.2:58529255-58529274 MS.gene066049:CDS 50.0%
ATCTGCAGCATCATCGCCTA+CGG + chr1.2:58529640-58529659 MS.gene066049:CDS 50.0%
ATCTGCTTCTCGGCATCTGA+AGG - chr1.2:58529715-58529734 None:intergenic 50.0%
ATGGAACCTTCTGCCTTCTC+TGG + chr1.2:58530070-58530089 MS.gene066049:CDS 50.0%
ATGGCAACTGAGCTAGCAGA+AGG - chr1.2:58530052-58530071 None:intergenic 50.0%
ATTGAGTTCATTGCCGGGGA+TGG - chr1.2:58530128-58530147 None:intergenic 50.0%
CATGATCCCTTCCGAAACTC+AGG - chr1.2:58529335-58529354 None:intergenic 50.0%
CGACCGTTGTATGCTTCTAC+CGG + chr1.2:58529497-58529516 MS.gene066049:CDS 50.0%
CGGAAGGGATCATGCAACAA+AGG + chr1.2:58529341-58529360 MS.gene066049:CDS 50.0%
CTGAATCTGAGTCTGCCACA+TGG - chr1.2:58529672-58529691 None:intergenic 50.0%
GATGCTGCAGATGGAGTCAA+AGG - chr1.2:58529632-58529651 None:intergenic 50.0%
GCAAACCCTTCTCATGCATC+TGG - chr1.2:58529450-58529469 None:intergenic 50.0%
GTCAGGTAGGGATGGATCAA+TGG - chr1.2:58529144-58529163 None:intergenic 50.0%
GTTGATGTTAACCGAGCGTG+CGG + chr1.2:58528825-58528844 MS.gene066049:CDS 50.0%
TCTGCTTCTCGGCATCTGAA+GGG - chr1.2:58529714-58529733 None:intergenic 50.0%
TTCTCGGCATCTGAAGGGTA+GGG - chr1.2:58529709-58529728 None:intergenic 50.0%
TTCTGCTAGCTCAGTTGCCA+TGG + chr1.2:58530051-58530070 MS.gene066049:CDS 50.0%
CCGCTGATGCAAACGGTAAC+CGG + chr1.2:58528949-58528968 MS.gene066049:CDS 55.0%
CCGGTTACCGTTTGCATCAG+CGG - chr1.2:58528952-58528971 None:intergenic 55.0%
CCTTCCGAAACTCAGGGCAA+GGG - chr1.2:58529328-58529347 None:intergenic 55.0%
CCTTCTGCCTTCTCTGGTTG+GGG + chr1.2:58530076-58530095 MS.gene066049:CDS 55.0%
CCTTGCCCTGAGTTTCGGAA+GGG + chr1.2:58529326-58529345 MS.gene066049:CDS 55.0%
CTTCTCGGCATCTGAAGGGT+AGG - chr1.2:58529710-58529729 None:intergenic 55.0%
GAGTGCCCCTTTGTTCATCC+TGG + chr1.2:58529254-58529273 MS.gene066049:CDS 55.0%
GCTGCAGATGGAGTCAAAGG+TGG - chr1.2:58529629-58529648 None:intergenic 55.0%
GGGTGAAGGTAGAGCAGAAC+CGG - chr1.2:58529519-58529538 None:intergenic 55.0%
GTAGGCGATGATGCTGCAGA+TGG - chr1.2:58529641-58529660 None:intergenic 55.0%
GTGCCCCTTTGTTCATCCTG+GGG + chr1.2:58529256-58529275 MS.gene066049:CDS 55.0%
GTGTCCCTTGCCCTGAGTTT+CGG + chr1.2:58529321-58529340 MS.gene066049:CDS 55.0%
TACAACGGTCGAAGCTCCTC+TGG - chr1.2:58529488-58529507 None:intergenic 55.0%
TCGGTTAACATCAACGCGGC+CGG - chr1.2:58528820-58528839 None:intergenic 55.0%
! GCTTTGGCTCCGCTTCGAAA+AGG - chr1.2:58529987-58530006 None:intergenic 55.0%
! TTGTTCTGCTGCTTCTGCCG+AGG + chr1.2:58528887-58528906 MS.gene066049:CDS 55.0%
AGGAGAGCCCCAACCAGAGA+AGG - chr1.2:58530086-58530105 None:intergenic 60.0%
CCCCAACCAGAGAAGGCAGA+AGG - chr1.2:58530079-58530098 None:intergenic 60.0%
CCCTTCCGAAACTCAGGGCA+AGG - chr1.2:58529329-58529348 None:intergenic 60.0%
CCCTTGCCCTGAGTTTCGGA+AGG + chr1.2:58529325-58529344 MS.gene066049:CDS 60.0%
TCTGGTTGGGGCTCTCCTGA+TGG + chr1.2:58530088-58530107 MS.gene066049:CDS 60.0%
TGCCACATGGTTCCAGCCGT+AGG - chr1.2:58529659-58529678 None:intergenic 60.0%
ACCGAGCGTGCGGCTCAGAT+AGG + chr1.2:58528835-58528854 MS.gene066049:CDS 65.0%
CCCTATCTGAGCCGCACGCT+CGG - chr1.2:58528839-58528858 None:intergenic 65.0%
CCGAGCGTGCGGCTCAGATA+GGG + chr1.2:58528836-58528855 MS.gene066049:CDS 65.0%
CGCCTACGGCTGGAACCATG+TGG + chr1.2:58529654-58529673 MS.gene066049:CDS 65.0%
GCAGCATCATCGCCTACGGC+TGG + chr1.2:58529644-58529663 MS.gene066049:CDS 65.0%
! CGGCGCCTTGCATTTTCCCC+AGG - chr1.2:58529275-58529294 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 58528525 58530291 58528525 ID=MS.gene066049
chr1.2 mRNA 58528525 58530291 58528525 ID=MS.gene066049.t1;Parent=MS.gene066049
chr1.2 exon 58528525 58530291 58528525 ID=MS.gene066049.t1.exon1;Parent=MS.gene066049.t1
chr1.2 CDS 58528525 58530291 58528525 ID=cds.MS.gene066049.t1;Parent=MS.gene066049.t1
Gene Sequence

>MS.gene066049

ATGTGCAACGGTTCGAATAGAAAACCTTGTCAAACTGGTTTGATTAGGGAAAATGAATGTGGAACACAAGAAGAACTTCATCATAAGATTTCACCTTTGCTTGAGTTTTCAGCCAAAGATGATATAATGGCGTTCACAAATGCTGTTGAAAAAGATGATCATGATGTTGATGAAGTAGGGTTATGGTATGGAAGGAAGGTTGGATCAAAAGAAATGGGATATGAAGAGAGGACACCTCTTATGGTTGCTGCTTTGTTTGGAAGCAAGGGTGTGTTGTCTTATATTCTTGGAACCGGCCGCGTTGATGTTAACCGAGCGTGCGGCTCAGATAGGGCTACCGCTCTTCATTGTGCTGTTTCTGGTTGTTCTGCTGCTTCTGCCGAGGTTGTCAAACTTTTGCTTGATGCATCTGCAGATGTTAGTTCCGCTGATGCAAACGGTAACCGGTGCAGTGACTTGATTGTCTCGGTTTCTAAGAGTATCTTCGGTTCAAGGAAGAGGATACTACAAGGCAATGTTGATGACGAGGACGACGATTTCCTTAAGAAAATAGGTTTCCAAATGGTGGAGCAACAACAAGATGTAGGTACATCTCGAACTGAGAAGAAAGATCATCCCATTGATCCATCCCTACCTGACATAAAGAATGGAATATATAGTACAGATGAATTTAGGATGTTTACATTCAAAGTGAAGCCTTGTTCAAGGACTTATACTCATGATTGGACTGAGTGCCCCTTTGTTCATCCTGGGGAAAATGCAAGGCGCCGTGATCCGATGAAATATCATTACACATGTGTCCCTTGCCCTGAGTTTCGGAAGGGATCATGCAACAAAGGGGATGCTTGTGAATATGCACATGGTATTTTTGAATGTTGGCTTCATCCTGCTCAATACAGAACAAGGCTTTGCAAGGATGAAACCAGATGCATGAGAAGGGTTTGCTTTTTCGCTCACAAACCAGAGGAGCTTCGACCGTTGTATGCTTCTACCGGTTCTGCTCTACCTTCACCCACATCATATTCAAATTCACCAAGTGCTTCTTCAATGGATTCTTTCACATTGAGCTCTCCGTCTTCTTTGATACAATCTGCGTCGACGCCACCTTTGACTCCATCTGCAGCATCATCGCCTACGGCTGGAACCATGTGGCAGACTCAGATTCAGCTTCATGCTGCAGTCCCTACCCTTCAGATGCCGAGAAGCAGATTTAAAACTGCAATGAATGCTAGAAATAATGCTGAATTTCTCGAACTTGAAAATCGCCTTACAGGTCTTGCATCTCCTTCAAATAGGCTTGCAGGAGTGAATCCTACTAACCTTGAAAACATTTTCGGGTCATCAATACAATCTCCGACATCAATACAAGTGCATCAGAACGCAAACCAGCAACTTTGGGGCAATCCTTTTGATCTTACCAATTCAAATGTGATTGCATCATCACAATTTAGAGTTGATGCCTTTTCGAAGCGGAGCCAAAGCTTTATTGAGCGCAACAGCGTTGCGAGCTTTAATTCTGAGCTTCCTTCTGCTAGCTCAGTTGCCATGGAACCTTCTGCCTTCTCTGGTTGGGGCTCTCCTGATGGAAAATTAGACTGGTCCATCCCCGGCAATGAACTCAATAAAATGAGGAAATCTTACAGAAGTAGCACTTCAACAATGGCTGCTGCATTAAGTGTTAATGATCCAGATATATTTTTAAGCCATGAATCTTGGGTTGATTCACTTGTGAATGATGGTCCAACAATGGAATTTGATCAACATTGA
Protein sequence

>MS.gene066049.t1

MCNGSNRKPCQTGLIRENECGTQEELHHKISPLLEFSAKDDIMAFTNAVEKDDHDVDEVGLWYGRKVGSKEMGYEERTPLMVAALFGSKGVLSYILGTGRVDVNRACGSDRATALHCAVSGCSAASAEVVKLLLDASADVSSADANGNRCSDLIVSVSKSIFGSRKRILQGNVDDEDDDFLKKIGFQMVEQQQDVGTSRTEKKDHPIDPSLPDIKNGIYSTDEFRMFTFKVKPCSRTYTHDWTECPFVHPGENARRRDPMKYHYTCVPCPEFRKGSCNKGDACEYAHGIFECWLHPAQYRTRLCKDETRCMRRVCFFAHKPEELRPLYASTGSALPSPTSYSNSPSASSMDSFTLSSPSSLIQSASTPPLTPSAASSPTAGTMWQTQIQLHAAVPTLQMPRSRFKTAMNARNNAEFLELENRLTGLASPSNRLAGVNPTNLENIFGSSIQSPTSIQVHQNANQQLWGNPFDLTNSNVIASSQFRVDAFSKRSQSFIERNSVASFNSELPSASSVAMEPSAFSGWGSPDGKLDWSIPGNELNKMRKSYRSSTSTMAAALSVNDPDIFLSHESWVDSLVNDGPTMEFDQH