AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066070.t1 MTR_1g111310 89.532 363 25 2 7 361 351 708 0.0 636
MS.gene066070.t1 MTR_1g111310 86.431 339 33 3 16 354 1 326 0.0 545
MS.gene066070.t1 MTR_5g054900 71.667 300 76 3 65 360 59 353 4.65e-155 440
MS.gene066070.t1 MTR_5g054900 71.667 300 76 3 65 360 140 434 5.76e-154 440
MS.gene066070.t1 MTR_5g054900 70.667 300 75 4 65 360 59 349 1.79e-150 428
MS.gene066070.t1 MTR_5g054900 70.667 300 75 4 65 360 170 460 7.24e-149 428
MS.gene066070.t1 MTR_8g040120 61.891 349 112 7 16 360 135 466 4.59e-144 416
MS.gene066070.t1 MTR_7g089800 64.650 314 95 4 58 360 115 423 6.12e-143 412
MS.gene066070.t1 MTR_7g089800 64.650 314 95 4 58 360 144 452 6.74e-143 412
MS.gene066070.t1 MTR_3g044820 88.945 199 16 2 39 231 10 208 2.39e-128 367
MS.gene066070.t1 MTR_7g092750 50.774 323 146 4 51 360 40 362 4.10e-114 336
MS.gene066070.t1 MTR_7g092750 50.774 323 146 4 51 360 141 463 5.68e-113 337
MS.gene066070.t1 MTR_3g087940 55.401 287 124 2 74 360 79 361 5.68e-109 323
MS.gene066070.t1 MTR_2g086420 52.265 287 126 2 74 360 194 469 4.54e-107 323
MS.gene066070.t1 MTR_2g086420 52.265 287 126 2 74 360 193 468 4.76e-107 323
MS.gene066070.t1 MTR_4g053250 51.000 300 133 2 61 360 176 461 1.60e-104 316
MS.gene066070.t1 MTR_0126s0080 56.000 250 106 3 69 314 211 460 4.20e-96 294
MS.gene066070.t1 MTR_7g092750 46.130 323 135 5 51 360 141 437 1.38e-94 289
MS.gene066070.t1 MTR_8g091650 46.831 284 146 3 77 360 5 283 2.55e-86 263
MS.gene066070.t1 MTR_8g091650 46.528 288 149 3 73 360 74 356 9.85e-86 263
MS.gene066070.t1 MTR_8g091650 46.690 287 148 3 74 360 111 392 5.03e-85 263
MS.gene066070.t1 MTR_5g060950 48.097 289 144 3 69 356 62 345 4.48e-84 259
MS.gene066070.t1 MTR_3g073560 45.545 303 155 4 59 358 50 345 7.41e-84 266
MS.gene066070.t1 MTR_1g111320 89.928 139 9 1 7 145 84 217 1.96e-83 253
MS.gene066070.t1 MTR_1g090370 32.381 210 111 7 139 342 33 217 3.44e-20 88.6
MS.gene066070.t1 MTR_2g076140 26.009 223 128 4 147 349 4 209 1.08e-14 72.8
MS.gene066070.t1 MTR_8g094690 25.424 236 144 5 135 338 3 238 1.23e-14 73.6
MS.gene066070.t1 MTR_7g068860 24.057 212 154 4 146 350 8 219 2.74e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066070.t1 AT1G68640 63.352 352 111 4 12 360 116 452 1.68e-140 407
MS.gene066070.t1 AT5G06950 63.091 317 104 7 50 360 21 330 6.18e-134 385
MS.gene066070.t1 AT5G06950 63.091 317 104 7 50 360 21 330 6.18e-134 385
MS.gene066070.t1 AT5G06950 63.091 317 104 7 50 360 21 330 6.18e-134 385
MS.gene066070.t1 AT5G06950 63.091 317 104 7 50 360 21 330 6.18e-134 385
MS.gene066070.t1 AT5G06950 63.091 317 104 7 50 360 21 330 6.18e-134 385
MS.gene066070.t1 AT3G12250 57.184 348 128 5 16 360 1 330 1.12e-132 382
MS.gene066070.t1 AT3G12250 57.184 348 128 5 16 360 1 330 1.12e-132 382
MS.gene066070.t1 AT3G12250 57.184 348 128 5 16 360 1 330 1.12e-132 382
MS.gene066070.t1 AT3G12250 57.184 348 128 5 16 360 1 330 1.12e-132 382
MS.gene066070.t1 AT3G12250 55.647 363 135 6 16 360 1 355 2.09e-131 380
MS.gene066070.t1 AT3G12250 62.295 305 104 4 59 360 7 303 4.80e-131 377
MS.gene066070.t1 AT3G12250 62.295 305 104 4 59 360 28 324 1.05e-130 377
MS.gene066070.t1 AT5G06960 61.301 292 105 3 72 360 44 330 1.36e-122 357
MS.gene066070.t1 AT5G06960 61.301 292 105 3 72 360 44 330 1.36e-122 357
MS.gene066070.t1 AT5G06960 61.301 292 105 3 72 360 24 310 1.95e-122 355
MS.gene066070.t1 AT1G08320 54.455 303 125 3 59 360 39 329 2.37e-115 339
MS.gene066070.t1 AT1G08320 54.455 303 125 3 59 360 39 329 2.37e-115 339
MS.gene066070.t1 AT1G08320 54.455 303 125 3 59 360 163 453 7.99e-114 340
MS.gene066070.t1 AT1G08320 54.455 303 125 3 59 360 163 453 7.99e-114 340
MS.gene066070.t1 AT1G08320 54.455 303 125 3 59 360 163 453 7.99e-114 340
MS.gene066070.t1 AT5G10030 54.007 287 128 2 74 360 80 362 3.52e-105 313
MS.gene066070.t1 AT5G10030 54.007 287 128 2 74 360 80 362 3.52e-105 313
MS.gene066070.t1 AT5G10030 54.007 287 128 2 74 360 80 362 3.52e-105 313
MS.gene066070.t1 AT5G10030 54.007 287 128 2 74 360 80 362 3.52e-105 313
MS.gene066070.t1 AT5G10030 54.007 287 128 2 74 360 80 362 3.52e-105 313
MS.gene066070.t1 AT5G06839 49.351 308 135 3 69 360 113 415 7.22e-104 312
MS.gene066070.t1 AT5G06839 49.351 308 135 3 69 360 156 458 1.06e-103 313
MS.gene066070.t1 AT5G06839 49.671 304 141 3 69 360 113 416 4.00e-103 310
MS.gene066070.t1 AT1G77920 47.042 355 168 6 9 360 29 366 1.23e-100 302
MS.gene066070.t1 AT1G22070 52.778 288 134 2 74 361 98 383 4.74e-100 301
MS.gene066070.t1 AT5G65210 51.220 287 136 2 74 360 14 296 3.33e-98 293
MS.gene066070.t1 AT5G65210 51.220 287 136 2 74 360 84 366 2.06e-97 294
MS.gene066070.t1 AT5G65210 51.220 287 136 2 74 360 84 366 2.06e-97 294
MS.gene066070.t1 AT5G65210 51.220 287 136 2 74 360 84 366 2.06e-97 294
MS.gene066070.t1 AT5G65210 51.220 287 136 2 74 360 84 366 2.06e-97 294
MS.gene066070.t1 AT5G65210 51.220 287 136 2 74 360 84 366 2.06e-97 294
MS.gene066070.t1 AT5G65210 51.220 287 136 2 74 360 84 366 2.06e-97 294
MS.gene066070.t1 AT3G14880 24.286 210 121 4 154 338 17 213 3.33e-12 66.2

Find 77 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGTATTTCACATACTTTC+TGG 0.133806 1.4:-84949521 MS.gene066070:CDS
TTCGAGCTCTTAATTCATTA+TGG 0.231768 1.4:-84948709 MS.gene066070:CDS
ATTGTTGGAAATAGTAATTC+AGG 0.251776 1.4:-84950006 MS.gene066070:intron
CAATTCCTTTAGTATTCTTT+AGG 0.266207 1.4:+84948677 None:intergenic
GAAAAGTAGGTTAAGGAAAA+AGG 0.288507 1.4:-84950867 MS.gene066070:intron
CCGTTCTTCCGAACTTCTTA+AGG 0.301460 1.4:-84949450 MS.gene066070:intron
AGGTGCATTAGCATTTGATA+TGG 0.316721 1.4:-84949702 MS.gene066070:CDS
ATGTATTTCACATACTTTCT+GGG 0.339033 1.4:-84949520 MS.gene066070:CDS
AAACCACCTTGAGCCGTTAT+CGG 0.365933 1.4:-84949147 MS.gene066070:CDS
CTTAGTCTTGCATTGATCCT+TGG 0.372651 1.4:+84951063 None:intergenic
ATCAGCTATAAATTCTCAAA+TGG 0.381451 1.4:-84949630 MS.gene066070:CDS
ATTGGTTCAGCTTCTTCTTC+AGG 0.387251 1.4:-84951313 MS.gene066070:CDS
TGGCCATTGCAAGAGCCATT+TGG 0.388199 1.4:+84948973 None:intergenic
TTCACATACTTTCTGGGTTG+TGG 0.392509 1.4:-84949514 MS.gene066070:CDS
ACCATGATCCGCTGGAACTC+CGG 0.404015 1.4:+84950035 None:intergenic
TCCTCCACATCCACTGGATC+TGG 0.420633 1.4:-84949017 MS.gene066070:CDS
GATCATGGTCATTCAATTGT+TGG 0.432278 1.4:-84950021 MS.gene066070:CDS
TAGGGTGCATTTGTTGCAAC+CGG 0.435060 1.4:-84950054 MS.gene066070:CDS
CAAAATCACCTTAAGAAGTT+CGG 0.438279 1.4:+84949442 None:intergenic
ACTAGAGACTTTCATTCATC+AGG 0.451246 1.4:-84948940 MS.gene066070:intron
AGTGTTTATCCACCGTGTCT+TGG 0.455646 1.4:-84951126 MS.gene066070:CDS
AGTCTCTAGTGTGGCAAGCT+TGG 0.468295 1.4:+84948953 None:intergenic
TTCAGCTTCTTCTTCAGGCA+TGG 0.471184 1.4:-84951308 MS.gene066070:CDS
CAAGGATCAATGCAAGACTA+AGG 0.474390 1.4:-84951062 MS.gene066070:CDS
ATAGGCATATGTGCAACAAT+TGG 0.478088 1.4:-84950236 MS.gene066070:intron
TTGATATGGATTATGCTCGT+TGG 0.480817 1.4:-84949688 MS.gene066070:CDS
GCTTCAACAAGTACGTCAAC+AGG 0.483846 1.4:-84950176 MS.gene066070:intron
TGATATGGATTATGCTCGTT+GGG 0.484946 1.4:-84949687 MS.gene066070:CDS
TCAGCTATAAATTCTCAAAT+GGG 0.485434 1.4:-84949629 MS.gene066070:CDS
GAACTGCATCTTCTTGTTGA+AGG 0.485780 1.4:-84949599 MS.gene066070:CDS
TGGAAATGTTGCTGAGTACA+TGG 0.493141 1.4:-84948997 MS.gene066070:CDS
CAAGGAGAGCGCGAGCAGCT+TGG 0.498749 1.4:+84948756 None:intergenic
ATCAACTGCTGATCCGATAA+CGG 0.503931 1.4:+84949134 None:intergenic
AATTGGAGAACAGTCGACTT+AGG 0.507628 1.4:-84950219 MS.gene066070:CDS
TTTCTTAGGCTGATCTCTTG+AGG 0.513370 1.4:-84948820 MS.gene066070:intron
ACCGGAGTTCCAGCGGATCA+TGG 0.518939 1.4:-84950036 MS.gene066070:CDS
TTATCGGATCAGCAGTTGAT+GGG 0.521698 1.4:-84949131 MS.gene066070:CDS
ATTTCCAGATCCAGTGGATG+TGG 0.524593 1.4:+84949013 None:intergenic
CATGTCCTAAAGAATACTAA+AGG 0.527396 1.4:-84948682 None:intergenic
ACACTATCCTCCACATCCAC+TGG 0.532462 1.4:-84949023 MS.gene066070:CDS
CACATGAAACATCTTTCTGC+AGG 0.533251 1.4:+84949485 None:intergenic
CTGCAAGGAAAAGTAGGTTA+AGG 0.538526 1.4:-84950874 MS.gene066070:CDS
TGCTGAGTACATGGGCCAAA+TGG 0.539603 1.4:-84948988 MS.gene066070:CDS
CCTTAAGAAGTTCGGAAGAA+CGG 0.540585 1.4:+84949450 None:intergenic
GTTATCGGATCAGCAGTTGA+TGG 0.541892 1.4:-84949132 MS.gene066070:CDS
TCCACCGTGTCTTGGTGCAA+TGG 0.545619 1.4:-84951118 MS.gene066070:CDS
TCTTGTAGTTGTTGAATCCA+AGG 0.547828 1.4:-84951080 MS.gene066070:CDS
GTTGAAGAGCATAGCTGCAA+AGG 0.551361 1.4:-84949546 MS.gene066070:CDS
GTGCAATGGTGTTGGAAATG+AGG 0.552383 1.4:-84951104 MS.gene066070:CDS
GGGCCAAATGGCTCTTGCAA+TGG 0.552891 1.4:-84948976 MS.gene066070:CDS
GTGAGGCTGCAAGGAAAAGT+AGG 0.555483 1.4:-84950880 MS.gene066070:CDS
TGCAGAATCGTGAGGCTGCA+AGG 0.558257 1.4:-84950889 MS.gene066070:CDS
AGCGCGAGCAGCTTGGTGCG+CGG 0.560510 1.4:+84948763 None:intergenic
GCTGAGGATGCTTTATCTCA+AGG 0.575247 1.4:-84949077 MS.gene066070:CDS
ATTGAATGACCATGATCCGC+TGG 0.583662 1.4:+84950027 None:intergenic
GCTGATCCGATAACGGCTCA+AGG 0.599388 1.4:+84949141 None:intergenic
GGATGCTTTATCTCAAGGAA+TGG 0.606203 1.4:-84949072 MS.gene066070:CDS
TTGTGTATTGTATAGAACAT+TGG 0.607394 1.4:-84951331 MS.gene066070:intron
TGTTGCAACCGGAGTTCCAG+CGG 0.611050 1.4:-84950043 MS.gene066070:CDS
GTGTCTTGGTGCAATGGTGT+TGG 0.611763 1.4:-84951112 MS.gene066070:CDS
AGCAACATTTCCAGATCCAG+TGG 0.612783 1.4:+84949007 None:intergenic
AAGGATCAATGCAAGACTAA+GGG 0.612820 1.4:-84951061 MS.gene066070:CDS
GAAAGATGTTTCATGTGGCT+CGG 0.616317 1.4:-84949479 MS.gene066070:CDS
GCATTCTTCTCAGCAAGCTG+AGG 0.619429 1.4:-84949093 MS.gene066070:CDS
TCCAGATCCAGTGGATGTGG+AGG 0.620584 1.4:+84949016 None:intergenic
AACACCATTGCACCAAGACA+CGG 0.624897 1.4:+84951114 None:intergenic
TATGGATTATGCTCGTTGGG+TGG 0.627032 1.4:-84949684 MS.gene066070:CDS
AAATGAAATCATTTATGACA+AGG 0.642100 1.4:+84948738 None:intergenic
AGATGTTTCATGTGGCTCGG+TGG 0.642187 1.4:-84949476 MS.gene066070:CDS
GGGTCAATCTGATGAGCACA+AGG 0.645680 1.4:-84951041 MS.gene066070:intron
TATCGGATCAGCAGTTGATG+GGG 0.649177 1.4:-84949130 MS.gene066070:CDS
TCGGCTGATGCAGAATCGTG+AGG 0.665525 1.4:-84950897 MS.gene066070:CDS
CTGCAGAAAGATGTTTCATG+TGG 0.677608 1.4:-84949484 MS.gene066070:CDS
GGAAATGTTGCTGAGTACAT+GGG 0.694330 1.4:-84948996 MS.gene066070:CDS
ATGAATGAAAGTCTCTAGTG+TGG 0.704515 1.4:+84948944 None:intergenic
ACCATTGCACCAAGACACGG+TGG 0.708421 1.4:+84951117 None:intergenic
GATCCGATAACGGCTCAAGG+TGG 0.733902 1.4:+84949144 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATAATTTAAATTTGAAT+TGG + chr1.4:84949183-84949202 None:intergenic 10.0%
!!! TAAAATAGAAATTGTATTTT+GGG - chr1.4:84950828-84950847 MS.gene066070:intron 10.0%
!! AAGTAATTAACTAACTTAAA+TGG - chr1.4:84950907-84950926 MS.gene066070:CDS 15.0%
!! TTACTGATACATTTATTTAT+GGG - chr1.4:84949212-84949231 MS.gene066070:intron 15.0%
!!! GTAAAATAGAAATTGTATTT+TGG - chr1.4:84950827-84950846 MS.gene066070:intron 15.0%
!!! TACTGATAAAACATTTTAAT+TGG - chr1.4:84949802-84949821 MS.gene066070:intron 15.0%
!!! TGCATTTTACAAATTAATTA+TGG - chr1.4:84950387-84950406 MS.gene066070:intron 15.0%
!! AAATGAAATCATTTATGACA+AGG + chr1.4:84951419-84951438 None:intergenic 20.0%
!! ATACATAGATATTGAAATAG+TGG + chr1.4:84948969-84948988 None:intergenic 20.0%
!! GTTACTGATACATTTATTTA+TGG - chr1.4:84949211-84949230 MS.gene066070:intron 20.0%
!! TACATAGATATTGAAATAGT+GGG + chr1.4:84948968-84948987 None:intergenic 20.0%
!! TAGGAAAGATTATTATAAAG+TGG - chr1.4:84950856-84950875 MS.gene066070:intron 20.0%
!! TATTATAAAGTGGTTATATC+AGG - chr1.4:84950866-84950885 MS.gene066070:intron 20.0%
!!! AACTTCAAAGATTTTTCATT+TGG - chr1.4:84950007-84950026 MS.gene066070:CDS 20.0%
!!! GATTTTTCATTTGGTAAAAT+TGG - chr1.4:84950016-84950035 MS.gene066070:CDS 20.0%
!!! GTATATTTTAATCTGTCAAA+GGG - chr1.4:84949698-84949717 MS.gene066070:CDS 20.0%
!!! TACTTAGTTTGTTAGTATAT+AGG + chr1.4:84950892-84950911 None:intergenic 20.0%
!!! TATATGTTTTGGTTTTGAAT+AGG - chr1.4:84949900-84949919 MS.gene066070:intron 20.0%
! ATCAGCTATAAATTCTCAAA+TGG - chr1.4:84950524-84950543 MS.gene066070:intron 25.0%
! ATGTATTTCACATACTTTCT+GGG - chr1.4:84950634-84950653 MS.gene066070:intron 25.0%
! ATTGTTGGAAATAGTAATTC+AGG - chr1.4:84950148-84950167 MS.gene066070:intron 25.0%
! TCAGCTATAAATTCTCAAAT+GGG - chr1.4:84950525-84950544 MS.gene066070:intron 25.0%
! TGGATAAACACTGAAAAATA+AGG + chr1.4:84949020-84949039 None:intergenic 25.0%
! TTGTGTATTGTATAGAACAT+TGG - chr1.4:84948823-84948842 MS.gene066070:intron 25.0%
!! AACCTTCTGAAAAATTGTTA+TGG - chr1.4:84949514-84949533 MS.gene066070:CDS 25.0%
!!! AATTGTATTTTGGGATGAAT+AGG - chr1.4:84950837-84950856 MS.gene066070:intron 25.0%
!!! ATGAAGTATTTTAAACCTTC+AGG - chr1.4:84950432-84950451 MS.gene066070:intron 25.0%
!!! ATTTTAGTGTTAAGCTAAGA+TGG + chr1.4:84950748-84950767 None:intergenic 25.0%
!!! CATTCTACTTATTTTTTCCA+TGG - chr1.4:84950062-84950081 MS.gene066070:intron 25.0%
!!! GATCATCTAATTTGACTTTT+GGG - chr1.4:84949614-84949633 MS.gene066070:CDS 25.0%
!!! GGTATATTTTAATCTGTCAA+AGG - chr1.4:84949697-84949716 MS.gene066070:CDS 25.0%
!!! TGAAAAATCTTTGAAGTTGT+TGG + chr1.4:84950005-84950024 None:intergenic 25.0%
!!! TGATCATCTAATTTGACTTT+TGG - chr1.4:84949613-84949632 MS.gene066070:CDS 25.0%
!!! TTGTGAACATTGTTTTTCTT+AGG - chr1.4:84951320-84951339 MS.gene066070:intron 25.0%
!!! TTTATGGGTTACATTTTCTT+AGG - chr1.4:84949227-84949246 MS.gene066070:intron 25.0%
AAGACAAATCATAAGTTAGG+AGG + chr1.4:84949326-84949345 None:intergenic 30.0%
AATGGACACTTAAATTCATG+CGG - chr1.4:84950346-84950365 MS.gene066070:intron 30.0%
ACACAACATTGAATCAGATA+AGG + chr1.4:84948809-84948828 None:intergenic 30.0%
AGATTAAAATATACCAGAGC+AGG + chr1.4:84949692-84949711 None:intergenic 30.0%
ATGAAAAAACAAATGCGAAG+AGG + chr1.4:84950967-84950986 None:intergenic 30.0%
CAAAATCACCTTAAGAAGTT+CGG + chr1.4:84950715-84950734 None:intergenic 30.0%
CACAAGACAAATCATAAGTT+AGG + chr1.4:84949329-84949348 None:intergenic 30.0%
GAAAAGTAGGTTAAGGAAAA+AGG - chr1.4:84949287-84949306 MS.gene066070:intron 30.0%
GATGTATTTCACATACTTTC+TGG - chr1.4:84950633-84950652 MS.gene066070:intron 30.0%
GCAACAAAATAGATGATCAA+TGG - chr1.4:84950328-84950347 MS.gene066070:intron 30.0%
TAATTGGACCTTTCTCTAAT+AGG - chr1.4:84949818-84949837 MS.gene066070:intron 30.0%
TACATTTGATGTATGAAGAG+TGG - chr1.4:84948988-84949007 MS.gene066070:CDS 30.0%
TCCTTAGAACTAATCATTCT+TGG - chr1.4:84950302-84950321 MS.gene066070:intron 30.0%
TGATCACACTCAATTAATTG+TGG + chr1.4:84951289-84951308 None:intergenic 30.0%
TTCGAGCTCTTAATTCATTA+TGG - chr1.4:84951445-84951464 MS.gene066070:intron 30.0%
! CTGGACTAAGTTATATGTTT+TGG - chr1.4:84949889-84949908 MS.gene066070:intron 30.0%
! GTGAAAAGTTTCAAAAGTGT+CGG - chr1.4:84950801-84950820 MS.gene066070:intron 30.0%
!! ACCAAGAATGATTAGTTCTA+AGG + chr1.4:84950306-84950325 None:intergenic 30.0%
!!! TCTAATTTGACTTTTGGGAT+TGG - chr1.4:84949619-84949638 MS.gene066070:CDS 30.0%
AAAAACAAATGCGAAGAGGA+AGG + chr1.4:84950963-84950982 None:intergenic 35.0%
AAAGACGGTTGAAATGTACT+TGG + chr1.4:84948936-84948955 None:intergenic 35.0%
AAGGATCAATGCAAGACTAA+GGG - chr1.4:84949093-84949112 MS.gene066070:CDS 35.0%
ACTAGAGACTTTCATTCATC+AGG - chr1.4:84951214-84951233 MS.gene066070:intron 35.0%
AGGTGCATTAGCATTTGATA+TGG - chr1.4:84950452-84950471 MS.gene066070:intron 35.0%
AGTGGGAAAAGATCAAAAGA+CGG + chr1.4:84948951-84948970 None:intergenic 35.0%
AGTTAACCCGAAAAAGAACA+TGG + chr1.4:84949434-84949453 None:intergenic 35.0%
ATAGGCATATGTGCAACAAT+TGG - chr1.4:84949918-84949937 MS.gene066070:intron 35.0%
ATGAATGAAAGTCTCTAGTG+TGG + chr1.4:84951213-84951232 None:intergenic 35.0%
CTGAAAAATTGTTATGGCCA+AGG - chr1.4:84949520-84949539 MS.gene066070:CDS 35.0%
GATCATGGTCATTCAATTGT+TGG - chr1.4:84950133-84950152 MS.gene066070:intron 35.0%
GATGTCTTCCTATTAGAGAA+AGG + chr1.4:84949829-84949848 None:intergenic 35.0%
GCACAAAGTCACAATAAAAG+TGG + chr1.4:84949157-84949176 None:intergenic 35.0%
TCTTGTAGTTGTTGAATCCA+AGG - chr1.4:84949074-84949093 MS.gene066070:CDS 35.0%
TGTGAAGGAAATGAAACTCA+AGG + chr1.4:84950187-84950206 None:intergenic 35.0%
TTCGTACGATATAGCATTAC+TGG - chr1.4:84949870-84949889 MS.gene066070:intron 35.0%
! TGATATGGATTATGCTCGTT+GGG - chr1.4:84950467-84950486 MS.gene066070:intron 35.0%
! TTGATATGGATTATGCTCGT+TGG - chr1.4:84950466-84950485 MS.gene066070:intron 35.0%
!! GGCCATAACAATTTTTCAGA+AGG + chr1.4:84949519-84949538 None:intergenic 35.0%
!!! ATTTTGTTTTCTTCCTGCTC+TGG - chr1.4:84949676-84949695 MS.gene066070:CDS 35.0%
ATCAACTGCTGATCCGATAA+CGG + chr1.4:84951023-84951042 None:intergenic 40.0%
CAAGGATCAATGCAAGACTA+AGG - chr1.4:84949092-84949111 MS.gene066070:CDS 40.0%
CACATGAAACATCTTTCTGC+AGG + chr1.4:84950672-84950691 None:intergenic 40.0%
CCTTAAGAAGTTCGGAAGAA+CGG + chr1.4:84950707-84950726 None:intergenic 40.0%
CTGCAAGGAAAAGTAGGTTA+AGG - chr1.4:84949280-84949299 MS.gene066070:intron 40.0%
CTGCAGAAAGATGTTTCATG+TGG - chr1.4:84950670-84950689 MS.gene066070:intron 40.0%
GAAAGATGTTTCATGTGGCT+CGG - chr1.4:84950675-84950694 MS.gene066070:intron 40.0%
GAACTGCATCTTCTTGTTGA+AGG - chr1.4:84950555-84950574 MS.gene066070:intron 40.0%
GAGTTTCATTTCCTTCACAG+TGG - chr1.4:84950188-84950207 MS.gene066070:CDS 40.0%
GGAAATGTTGCTGAGTACAT+GGG - chr1.4:84951158-84951177 MS.gene066070:intron 40.0%
GGATGCTTTATCTCAAGGAA+TGG - chr1.4:84951082-84951101 MS.gene066070:CDS 40.0%
TATAGGGGAAAAGCAATCCA+TGG + chr1.4:84950082-84950101 None:intergenic 40.0%
TCAAATGCTAATGCACCTGA+AGG + chr1.4:84950450-84950469 None:intergenic 40.0%
TGCAACAAATGCACCCTATA+GGG + chr1.4:84950098-84950117 None:intergenic 40.0%
TGGAAATGTTGCTGAGTACA+TGG - chr1.4:84951157-84951176 MS.gene066070:intron 40.0%
TTATCGGATCAGCAGTTGAT+GGG - chr1.4:84951023-84951042 MS.gene066070:intron 40.0%
TTCACATACTTTCTGGGTTG+TGG - chr1.4:84950640-84950659 MS.gene066070:intron 40.0%
TTGCAACAAATGCACCCTAT+AGG + chr1.4:84950099-84950118 None:intergenic 40.0%
TTGTTATGGCCAAGGACATA+AGG - chr1.4:84949528-84949547 MS.gene066070:CDS 40.0%
TTTCTTAGGCTGATCTCTTG+AGG - chr1.4:84951334-84951353 MS.gene066070:intron 40.0%
! AATTGGAGAACAGTCGACTT+AGG - chr1.4:84949935-84949954 MS.gene066070:intron 40.0%
! ATTGGTTCAGCTTCTTCTTC+AGG - chr1.4:84948841-84948860 MS.gene066070:intron 40.0%
! CATTTTCTTAGGCTCTTCGT+CGG - chr1.4:84949238-84949257 MS.gene066070:intron 40.0%
! GTTGCAAGTAACTTGCAGTA+TGG - chr1.4:84948696-84948715 MS.gene066070:CDS 40.0%
! TCCAGCTCCATGTTCTTTTT+CGG - chr1.4:84949424-84949443 MS.gene066070:intron 40.0%
!! ATGGATTGCTTTTCCCCTAT+AGG - chr1.4:84950081-84950100 MS.gene066070:intron 40.0%
!! CTTAGTCTTGCATTGATCCT+TGG + chr1.4:84949094-84949113 None:intergenic 40.0%
!! TGGATTGCTTTTCCCCTATA+GGG - chr1.4:84950082-84950101 MS.gene066070:intron 40.0%
AAACCACCTTGAGCCGTTAT+CGG - chr1.4:84951007-84951026 MS.gene066070:intron 45.0%
AACACCATTGCACCAAGACA+CGG + chr1.4:84949043-84949062 None:intergenic 45.0%
ACACTCTTGCCTTATGTCCT+TGG + chr1.4:84949540-84949559 None:intergenic 45.0%
AGCAACATTTCCAGATCCAG+TGG + chr1.4:84951150-84951169 None:intergenic 45.0%
AGTGTTTATCCACCGTGTCT+TGG - chr1.4:84949028-84949047 MS.gene066070:CDS 45.0%
ATTGAATGACCATGATCCGC+TGG + chr1.4:84950130-84950149 None:intergenic 45.0%
ATTTCCAGATCCAGTGGATG+TGG + chr1.4:84951144-84951163 None:intergenic 45.0%
CCGTTCTTCCGAACTTCTTA+AGG - chr1.4:84950704-84950723 MS.gene066070:intron 45.0%
CCTTCACAGTGGTAGAAACA+TGG - chr1.4:84950199-84950218 MS.gene066070:CDS 45.0%
GCAACAAATGCACCCTATAG+GGG + chr1.4:84950097-84950116 None:intergenic 45.0%
GCTGAGGATGCTTTATCTCA+AGG - chr1.4:84951077-84951096 MS.gene066070:CDS 45.0%
GCTTCAACAAGTACGTCAAC+AGG - chr1.4:84949978-84949997 MS.gene066070:intron 45.0%
GTTATCGGATCAGCAGTTGA+TGG - chr1.4:84951022-84951041 MS.gene066070:intron 45.0%
TTCAGCTTCTTCTTCAGGCA+TGG - chr1.4:84948846-84948865 MS.gene066070:intron 45.0%
! CCAGCTCCATGTTCTTTTTC+GGG - chr1.4:84949425-84949444 MS.gene066070:intron 45.0%
! CCATGTTTCTACCACTGTGA+AGG + chr1.4:84950202-84950221 None:intergenic 45.0%
! GTGCAATGGTGTTGGAAATG+AGG - chr1.4:84949050-84949069 MS.gene066070:CDS 45.0%
! GTTGAAGAGCATAGCTGCAA+AGG - chr1.4:84950608-84950627 MS.gene066070:intron 45.0%
! TAGGGTGCATTTGTTGCAAC+CGG - chr1.4:84950100-84950119 MS.gene066070:intron 45.0%
! TATCGGATCAGCAGTTGATG+GGG - chr1.4:84951024-84951043 MS.gene066070:intron 45.0%
! TATGGATTATGCTCGTTGGG+TGG - chr1.4:84950470-84950489 MS.gene066070:intron 45.0%
ACACTATCCTCCACATCCAC+TGG - chr1.4:84951131-84951150 MS.gene066070:intron 50.0%
AGTCTCTAGTGTGGCAAGCT+TGG + chr1.4:84951204-84951223 None:intergenic 50.0%
CCCGAAAAAGAACATGGAGC+TGG + chr1.4:84949428-84949447 None:intergenic 50.0%
GCATTCTTCTCAGCAAGCTG+AGG - chr1.4:84951061-84951080 MS.gene066070:CDS 50.0%
GGGTCAATCTGATGAGCACA+AGG - chr1.4:84949113-84949132 MS.gene066070:CDS 50.0%
GTGAGGCTGCAAGGAAAAGT+AGG - chr1.4:84949274-84949293 MS.gene066070:intron 50.0%
TGCTGAGTACATGGGCCAAA+TGG - chr1.4:84951166-84951185 MS.gene066070:intron 50.0%
TGGCCATTGCAAGAGCCATT+TGG + chr1.4:84951184-84951203 None:intergenic 50.0%
! AGATGTTTCATGTGGCTCGG+TGG - chr1.4:84950678-84950697 MS.gene066070:intron 50.0%
! GTGTCTTGGTGCAATGGTGT+TGG - chr1.4:84949042-84949061 MS.gene066070:CDS 50.0%
ACCATGATCCGCTGGAACTC+CGG + chr1.4:84950122-84950141 None:intergenic 55.0%
ACCATTGCACCAAGACACGG+TGG + chr1.4:84949040-84949059 None:intergenic 55.0%
GGGCCAAATGGCTCTTGCAA+TGG - chr1.4:84951178-84951197 MS.gene066070:intron 55.0%
TCCAGATCCAGTGGATGTGG+AGG + chr1.4:84951141-84951160 None:intergenic 55.0%
TCCTCCACATCCACTGGATC+TGG - chr1.4:84951137-84951156 MS.gene066070:intron 55.0%
TCGGCTGATGCAGAATCGTG+AGG - chr1.4:84949257-84949276 MS.gene066070:intron 55.0%
TGCAGAATCGTGAGGCTGCA+AGG - chr1.4:84949265-84949284 MS.gene066070:intron 55.0%
TGTTGCAACCGGAGTTCCAG+CGG - chr1.4:84950111-84950130 MS.gene066070:intron 55.0%
! GATCCGATAACGGCTCAAGG+TGG + chr1.4:84951013-84951032 None:intergenic 55.0%
! GCTGATCCGATAACGGCTCA+AGG + chr1.4:84951016-84951035 None:intergenic 55.0%
! TCCACCGTGTCTTGGTGCAA+TGG - chr1.4:84949036-84949055 MS.gene066070:CDS 55.0%
ACCGGAGTTCCAGCGGATCA+TGG - chr1.4:84950118-84950137 MS.gene066070:intron 60.0%
! CAAGGAGAGCGCGAGCAGCT+TGG + chr1.4:84951401-84951420 None:intergenic 65.0%
!! AGCGCGAGCAGCTTGGTGCG+CGG + chr1.4:84951394-84951413 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 84948685 84951491 84948685 ID=MS.gene066070
chr1.4 mRNA 84948685 84951491 84948685 ID=MS.gene066070.t1;Parent=MS.gene066070
chr1.4 exon 84951474 84951491 84951474 ID=MS.gene066070.t1.exon1;Parent=MS.gene066070.t1
chr1.4 CDS 84951474 84951491 84951474 ID=cds.MS.gene066070.t1;Parent=MS.gene066070.t1
chr1.4 exon 84951240 84951338 84951240 ID=MS.gene066070.t1.exon2;Parent=MS.gene066070.t1
chr1.4 CDS 84951240 84951338 84951240 ID=cds.MS.gene066070.t1;Parent=MS.gene066070.t1
chr1.4 exon 84951042 84951146 84951042 ID=MS.gene066070.t1.exon3;Parent=MS.gene066070.t1
chr1.4 CDS 84951042 84951146 84951042 ID=cds.MS.gene066070.t1;Parent=MS.gene066070.t1
chr1.4 exon 84950868 84950927 84950868 ID=MS.gene066070.t1.exon4;Parent=MS.gene066070.t1
chr1.4 CDS 84950868 84950927 84950868 ID=cds.MS.gene066070.t1;Parent=MS.gene066070.t1
chr1.4 exon 84950177 84950254 84950177 ID=MS.gene066070.t1.exon5;Parent=MS.gene066070.t1
chr1.4 CDS 84950177 84950254 84950177 ID=cds.MS.gene066070.t1;Parent=MS.gene066070.t1
chr1.4 exon 84950007 84950073 84950007 ID=MS.gene066070.t1.exon6;Parent=MS.gene066070.t1
chr1.4 CDS 84950007 84950073 84950007 ID=cds.MS.gene066070.t1;Parent=MS.gene066070.t1
chr1.4 exon 84949451 84949722 84949451 ID=MS.gene066070.t1.exon7;Parent=MS.gene066070.t1
chr1.4 CDS 84949451 84949722 84949451 ID=cds.MS.gene066070.t1;Parent=MS.gene066070.t1
chr1.4 exon 84948941 84949177 84948941 ID=MS.gene066070.t1.exon8;Parent=MS.gene066070.t1
chr1.4 CDS 84948941 84949177 84948941 ID=cds.MS.gene066070.t1;Parent=MS.gene066070.t1
chr1.4 exon 84948685 84948834 84948685 ID=MS.gene066070.t1.exon9;Parent=MS.gene066070.t1
chr1.4 CDS 84948685 84948834 84948685 ID=cds.MS.gene066070.t1;Parent=MS.gene066070.t1
Gene Sequence

>MS.gene066070

ATGTCACTGTTGTTGCAAGTAACTTGCAGTATGGAACATTCAATACTGTAACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATCTAAATTTTGTGTAAAACTAACAGCCTTATCTGATTCAATGTTGTGTATTGTATAGAACATTGGTTCAGCTTCTTCTTCAGGCATGGCAGATAACAGTCAAAAGACAGAAGATACTTCAACAGAAGATTTTGACAATGATGATAAAAACAACCAAGTACATTTCAACCGTCTTTTGATCTTTTCCCACTATTTCAATATCTATGTATACATTTGATGTATGAAGAGTGGTAATGTCCTTATTTTTCAGTGTTTATCCACCGTGTCTTGGTGCAATGGTGTTGGAAATGAGGCTCTTGTAGTTGTTGAATCCAAGGATCAATGCAAGACTAAGGGTCAATCTGATGAGCACAAGGTTGTTGATTTTTTTTCTACCACTTTTATTGTGACTTTGTGCTATCCAATTCAAATTTAAATTATGTTACATTTCTGTTACTGATACATTTATTTATGGGTTACATTTTCTTAGGCTCTTCGTCGGCTGATGCAGAATCGTGAGGCTGCAAGGAAAAGTAGGTTAAGGAAAAAGGTAATTGCATTGATCCTCCTAACTTATGATTTGTCTTGTGTGTGTGTCTTGTTTGTATGTTATATTTAAACTAATTTCTGCTTTGATGTTTTTAGTCGAATAGAGAGCTTAATTATCCAGCTCCATGTTCTTTTTCGGGTTAACTTAACAAGTTCTTACAAACTTATAAACATTTATTAAGTAGTATTGTAATACTTAAATAAGAAACCTTCTGAAAAATTGTTATGGCCAAGGACATAAGGCAAGAGTGTTTTATAACTTATTAAGATGCAAAAGTTAATGTAATACTTTCATTTTTCATAAATGATCATCTAATTTGACTTTTGGGATTGGAAAAGCTATTCTTTTGCATTGAATTTGACTACAAATTTTGTTTTCTTCCTGCTCTGGTATATTTTAATCTGTCAAAGGGACTGCATAATGTTGATGTTTTAGACGTATACATAACTCAATAATTTCTGAACTGTTTTATGTCACGACGCTGTAACTCAAATACTGATAAAACATTTTAATTGGACCTTTCTCTAATAGGAAGACATCTCAGCATTTGTGACAAAAAAATTCGTACGATATAGCATTACTGGACTAAGTTATATGTTTTGGTTTTGAATAGGCATATGTGCAACAATTGGAGAACAGTCGACTTAGGCTTGCTCAAATAGAACAAGAGCTTCAACAAGTACGTCAACAGGTCCAACAACTTCAAAGATTTTTCATTTGGTAAAATTGGTTTGCTCAATATAATTTTATTGTCATTCTACTTATTTTTTCCATGGATTGCTTTTCCCCTATAGGGTGCATTTGTTGCAACCGGAGTTCCAGCGGATCATGGTCATTCAATTGTTGGAAATAGTAATTCAGGTGAAGTGAAGCAACCTTGAGTTTCATTTCCTTCACAGTGGTAGAAACATGGTGTTTGTGTCTAATATATGTTTTCAAATATAAATTACTCTATTATTTTTGATATTTTAACTGCAATTTATGATGTGCATTTCCTTAGAACTAATCATTCTTGGTAAGCAACAAAATAGATGATCAATGGACACTTAAATTCATGCGGCATCATGAGAATTAAGAATGCATTTTACAAATTAATTATGGAGAGAATGTGAGTATGTGACTGATGAAGTATTTTAAACCTTCAGGTGCATTAGCATTTGATATGGATTATGCTCGTTGGGTGGATGAGCATCAACGACTAATCAATGACATAAGATCAGCTATAAATTCTCAAATGGGCGATAATGAACTGCATCTTCTTGTTGAAGGTGTCATGTCACATTATGATGAATTATACAAGTTGAAGAGCATAGCTGCAAAGGCTGATGTATTTCACATACTTTCTGGGTTGTGGAAGACACCTGCAGAAAGATGTTTCATGTGGCTCGGTGGATTCCGTTCTTCCGAACTTCTTAAGGTGATTTTGATTTCATTTACCATCTTAGCTTAACACTAAAATTTGCTGCTCTTGTATAGAAAGCATTATTATAAAGTGAAAAGTTTCAAAAGTGTCGGAAAGTAAAATAGAAATTGTATTTTGGGATGAATAGGAAAGATTATTATAAAGTGGTTATATCAGGCCTATATACTAACAAACTAAGTAATTAACTAACTTAAATGGACTAGTAACTATTTACACTTTATAACTATTCCTTCCTCTTCGCATTTGTTTTTTCATATTGAGATTCAGATTGTTAGAAACCACCTTGAGCCGTTATCGGATCAGCAGTTGATGGGGATCTACAATCTGCAGCATTCTTCTCAGCAAGCTGAGGATGCTTTATCTCAAGGAATGGAAGCATTGCAACAATCTCTTTCAGAAACACTATCCTCCACATCCACTGGATCTGGAAATGTTGCTGAGTACATGGGCCAAATGGCTCTTGCAATGGCCAAGCTTGCCACACTAGAGACTTTCATTCATCAGGTAATTAAACTTAAGACAATTTGATCTTGATCACTCAACATTATTTTATTCCACAATTAATTGAGTGTGATCACATTGTAAATCTTGTGAACATTGTTTTTCTTAGGCTGATCTCTTGAGGCAACAAACACTGCAACAGTTGCGTCGAATTTTGACCGCGCACCAAGCTGCTCGCGCTCTCCTTGTCATAAATGATTTCATTTCGCGAATTCGAGCTCTTAATTCATTATGGTCAGCATGTCCTAAAGAATACTAA
Protein sequence

>MS.gene066070.t1

MSLLLQNIGSASSSGMADNSQKTEDTSTEDFDNDDKNNQCLSTVSWCNGVGNEALVVVESKDQCKTKGQSDEHKALRRLMQNREAARKSRLRKKAYVQQLENSRLRLAQIEQELQQVRQQGAFVATGVPADHGHSIVGNSNSGALAFDMDYARWVDEHQRLINDIRSAINSQMGDNELHLLVEGVMSHYDELYKLKSIAAKADVFHILSGLWKTPAERCFMWLGGFRSSELLKIVRNHLEPLSDQQLMGIYNLQHSSQQAEDALSQGMEALQQSLSETLSSTSTGSGNVAEYMGQMALAMAKLATLETFIHQADLLRQQTLQQLRRILTAHQAARALLVINDFISRIRALNSLWSACPKEY