AlfalfaGEDB | MS.gene066274

M. sativa cultivar XinJiangDaYe / MS.gene066274

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066274.t1	XP_013444518.1	79.4	412	80	3	5	415	61	468	5.30E-185	657.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066274.t1	Q9M126	40.9	242	114	7	143	371	6	231	5.8e-39	163.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066274.t1	G7ZZJ1	79.4	412	80	3	5	415	61	468	3.8e-185	657.1

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene066274.t1	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066274.t1	MTR_8g023900	85.956	413	53	3	4	415	60	468	0.0	735
MS.gene066274.t1	MTR_8g023900	63.298	188	67	2	130	315	1	188	7.30e-78	249
MS.gene066274.t1	MTR_8g023930	74.242	264	68	0	142	405	25	288	4.85e-144	419
MS.gene066274.t1	MTR_8g023930	68.702	131	37	2	4	134	72	198	3.73e-54	187
MS.gene066274.t1	MTR_8g023860	63.799	279	99	1	130	408	1	277	3.94e-123	363
MS.gene066274.t1	MTR_8g023860	63.780	127	42	2	6	132	62	184	6.85e-42	153
MS.gene066274.t1	MTR_8g023840	65.505	287	95	3	130	415	1	284	3.17e-122	360
MS.gene066274.t1	MTR_8g023840	65.354	127	40	2	6	132	61	183	2.18e-42	154
MS.gene066274.t1	MTR_8g023880	48.372	215	86	7	142	342	8	211	1.92e-53	179
MS.gene066274.t1	MTR_8g023880	46.610	118	41	5	6	109	57	166	1.49e-19	88.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066274.t1	AT4G01550	41.079	241	113	7	143	370	6	230	3.63e-46	165
MS.gene066274.t1	AT4G01550	40.278	144	77	5	6	147	54	190	2.58e-21	95.9
MS.gene066274.t1	AT4G35580	51.572	159	66	5	142	293	10	164	1.64e-45	165
MS.gene066274.t1	AT4G35580	47.170	106	52	3	6	109	58	161	2.70e-22	99.4
MS.gene066274.t1	AT4G35580	51.572	159	66	5	142	293	10	164	1.76e-45	165
MS.gene066274.t1	AT4G35580	47.170	106	52	3	6	109	58	161	2.87e-22	99.4
MS.gene066274.t1	AT4G35580	51.572	159	66	5	142	293	10	164	2.10e-45	164
MS.gene066274.t1	AT4G35580	47.170	106	52	3	6	109	58	161	3.62e-22	99.0
MS.gene066274.t1	AT3G49530	51.852	162	68	5	142	296	14	172	1.11e-43	157
MS.gene066274.t1	AT3G49530	41.045	134	72	5	6	133	62	194	1.55e-22	99.4

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Find 73 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATATGGAATCAATATTTG+AGG	0.513860	8.3:-68289042	MS.gene066274:CDS
AACAAACCTGGTGATCCTCA+AGG	0.591442	8.3:+68290177	None:intergenic
AACAGGAGGAACAAGAAGAA+TGG	0.482221	8.3:+68290671	None:intergenic
AACCTGGTGATCCTCAAGGG+TGG	0.685631	8.3:+68290181	None:intergenic
AAGAAGAATGGGAGGAAATG+AGG	0.527793	8.3:+68290683	None:intergenic
AAGTATCTGTCTGCTTGGAA+AGG	0.573950	8.3:+68289017	None:intergenic
AATAAAATTGAAGAGGAAGC+TGG	0.471203	8.3:-68289278	MS.gene066274:CDS
ACAAACCTGGTGATCCTCAA+GGG	0.612386	8.3:+68290178	None:intergenic
ACAAGTATGCAAAGAGTAAA+AGG	0.277265	8.3:-68290370	MS.gene066274:CDS
ACAGGAGGAACAAGAAGAAT+GGG	0.530636	8.3:+68290672	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTAAAAAAGAGTGGCT+TGG	+	chr8.3:68291190-68291209	None:intergenic	30.0%
AATAAAATTGAAGAGGAAGC+TGG	-	chr8.3:68291456-68291475	MS.gene066274:intron	30.0%
AATTCTTATCATGCATCTCA+TGG	+	chr8.3:68291318-68291337	None:intergenic	30.0%
ACAAGTATGCAAAGAGTAAA+AGG	-	chr8.3:68290364-68290383	MS.gene066274:CDS	30.0%
ACCAGTAGAATATGTATAAG+AGG	+	chr8.3:68291048-68291067	None:intergenic	30.0%
AGCTGAGAATAAAATTGAAG+AGG	-	chr8.3:68291449-68291468	MS.gene066274:intron	30.0%
ATACAATGATGTGTGATGAA+GGG	-	chr8.3:68290006-68290025	MS.gene066274:intron	30.0%
ATACTCATGAATGATCCAAT+TGG	+	chr8.3:68290526-68290545	None:intergenic	30.0%
ATATACAAGTGAAAAGAGCT+AGG	-	chr8.3:68290897-68290916	MS.gene066274:intron	30.0%
ATGAAAAAAGCCGAGATAAA+AGG	-	chr8.3:68289969-68289988	MS.gene066274:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	68288815	68291941	68288815	ID=MS.gene066274
chr8.3	mRNA	68288815	68291941	68288815	ID=MS.gene066274.t1;Parent=MS.gene066274
chr8.3	exon	68291932	68291941	68291932	ID=MS.gene066274.t1.exon1;Parent=MS.gene066274.t1
chr8.3	CDS	68291932	68291941	68291932	ID=cds.MS.gene066274.t1;Parent=MS.gene066274.t1
chr8.3	exon	68291554	68291831	68291554	ID=MS.gene066274.t1.exon2;Parent=MS.gene066274.t1
chr8.3	CDS	68291554	68291831	68291554	ID=cds.MS.gene066274.t1;Parent=MS.gene066274.t1
chr8.3	exon	68290535	68290811	68290535	ID=MS.gene066274.t1.exon3;Parent=MS.gene066274.t1
chr8.3	CDS	68290535	68290811	68290535	ID=cds.MS.gene066274.t1;Parent=MS.gene066274.t1
chr8.3	exon	68290184	68290449	68290184	ID=MS.gene066274.t1.exon4;Parent=MS.gene066274.t1
chr8.3	CDS	68290184	68290449	68290184	ID=cds.MS.gene066274.t1;Parent=MS.gene066274.t1
chr8.3	exon	68289193	68289339	68289193	ID=MS.gene066274.t1.exon5;Parent=MS.gene066274.t1
chr8.3	CDS	68289193	68289339	68289193	ID=cds.MS.gene066274.t1;Parent=MS.gene066274.t1
chr8.3	exon	68288815	68289087	68288815	ID=MS.gene066274.t1.exon6;Parent=MS.gene066274.t1
chr8.3	CDS	68288815	68289087	68288815	ID=cds.MS.gene066274.t1;Parent=MS.gene066274.t1

Gene Sequence

>MS.gene066274

ATGTATTCAAGTATAAACCTTGGGATTTACCAGGTAAATATCAAACAAACCCCACCATTAGTTTCTTATAATAATGTGATCTTTTTGATATATTATTCCTTCTTTTTCAGCTCATTCAGTGATAAGATCCGATGACTCGGAATGGTTTTTCTTGTGTCCTATTGAATACAAGTATGCAAAGAGCAATAAGCTTAAGAGAGCAACAAAGTTTGGATTTTGGAAAGCCACTGGAAAAGATCATTCGATTAAGAATAGAGGTACTGACGAAGTTATTGGGACGAAGAAGACTCTTGTTTACTACCTTGCTCCTCATCCTGGTGTTAAAACCAACTGGGTTATTCGTGAGTACCATGATGTTGTCAGTGTCAGATTTGATGATAATACACAGGTTTGTTAAGTTACAACATTGTTTTAACTTTTAATGTTGTCACATTAGTTTTTTTTTTCCTTTTTAAGGATTTGATATTTTCTATCCAATTGGTTGTTCTGAAATATGAGGATATTGGATTTATTGTTTTGAAATTTTGATTTTTGGACCCTTTTTCTTTTGGTATCATATTATATTGGTTACTTAATTTTCACAAAAATTTGTCACTTTCTGCTATACTAAGAAACTATTTTTGAGATGAAGTTTTTGATGAAAAGGTATTTTGCTTTATATCCAATCAAAATCACTCTTTATCAGGTTGTTTTGTATGAATTTGAAGCATTGACTTGATTTGTCTTTTTCTTTTTTTGACATAGGATTGATTTGTTTTGGTACTTATTTGTACTGAGTATAAAGTGAATATTGATTATCAACTCTGAGGTGATAACTCAGAAATTGAATTCCCTGCATTGATTGGGTTCATGCATTTGAGACATTGATCGGACGATTCACAAAAAAGAAGAGTTTCATTTTTATTTTATAATTCAAAGTACATAACATGAATCTAAAACGTCCGATCATGTTCGTCCCAAATGTGTGAGTACATGAAAAGCCGACTGCAGTGGAGCGAGCTCAGTAGTAAGGGATGTAATACATGCCTTTCTGAGATAGTTTTAAAATAATAATACTACTCTGATTAATAATAAGGTATCCTTTTCCAATTGTCTCTTTTAAATGTTGATTATGCTCCAAAATTTTGTAGAGGACTTTTGTTTTGTGTCGCTTGATGAAAAAAGCCGAGATAAAAGGCGAAGAGGAAAATGATACAATGATGTGTGATGAAGGGGAGCCTAGCAGACAAATGACAGCCTCATTTCCTCCCATTCTTCTTGTTCCTCCTGTTGGATTCAGATTTTGTCCAACAGATGAAGAGCTAATCAGTCACTACCTTAGAAACAAGTTGCTTGGTAATGACGCAATTGTAAACAATGTTATAGCTGAAGTTGATATTCGCAAGTTCGAACCATGGGAATTACCAGGTAAATATATAAAACAAATAAACAAATCCCATCATTATTTTGACACAAAAATGTGATTTTTATTACTGTTTTTGTTATTTTTCAGCTCGTTCAGTGATAAAGTCTGATGATCCGGAATGGTTTTTTGTGTGTCCTCTTAAATACAAGTATGCAAAGAGTAAAAGGATTAATAGGACAACAAAAAATGGTTTTTGGAAAGCTACTGGAATGGACCGTAACATTAGGATTAGGGGTACCGATAAAGTAATTGGGATTAAGAAGACTCTTGTTTACCATGAAAATCCTGTTCCTGCTGTTAAGACCAATTGGATCATTCATGAGTATCATGATGCCACCCTTGAGGATCACCAGGTTTGTTATGTTACATTTAAGGTTTTAACTTTTAATGCTTTTAATATGTAACATTTTAAAAAAAAAATATTCTTTAATCACTAGAAATGAAATGAGTATAGTATTGTATGTGTTTTTTTCTTTCAGGATTTGATATATTTCTTATCTGATAGGCTGTTCTGAAATTTGAAGATGATTGGATTCATTCTTTGAAAATTTGATTTTGAACCCCTTTTTCTTTTAGTGTCATATTGTGAGGATATCAAAACCAGAGAGAAATGAACAAAGAAATCTCAGATATTGGAATGAATGATCTAAACTGAAAGTTAGTTTCATGATTCATATATATACAAGTGAAAAGAGCTAGGTGACTTGGATCATAAGTAACCAATTATAACTAACTTTGAACTAGCTTATTTTGGCGTAACTAACTCTATTCTATCATCTATAATCTATATATGACTGATAATCACCTGTTGTTTACTAGTATTATCCTCTTATACATATTCTACTGGTTTCTCAAATTGGTTTTATTTTATGGAAAGATCCATCACTGTCTTTTTCTGCTATGCTATGTATGTAACTATCTTTAAGATGAAGATTGTGTTGAAATACTATTTTTCTTGATCATCATACCAAGCCACTCTTTTTTAGTTTTTGCTGTGAAGTTCATGAAACAATTTCAGATTTTGAAGCATTGGCTTGATTTGGACCTCTTGGTATTTATGTGTATGCATTTGTATAAAGTAAAAAGTTAACCGATCCATGAGATGCATGATAAGAATTTCACTTTGTAATCTCGGAGTTCGAGTTTAATTCCAATATGAGACATTATAAAATGTTGATTATCCTCCAAATTTTGTAGAGGACTTTTGTTTTGTGCCGCTTGATTAAGAAAGCTGAGAATAAAATTGAAGAGGAAGCTGGTATGATTATATGTGATGAAGGCGAGTCGAGTAGACACATGAGTTCTGACTACGAAAATCAAGAAACAGCTGAAGGAATTTCAGATGTAAGCAGTTCATCCTTGATAGGAATTTTCAAAACTAAGTCTTGGTTTTACACCTTTCAACATAAAACATGCTGAGAACTTGTACTTGTTTTATATCTGTTTCAGGTAAGTGGTACTTTGCCAGAAATAAATATGGAATCAATATTTGAGGAACCTTTCCAAGCAGACAGATACTTTCCCTACTCTACGCAACAATCTTCACTGAATGAAAATGAGCCGGAACTATTATTCTCGAACTCACAATTTTACAATGCCTATTTAAAAAATGAAAACAATGTTATCCAGAGCATGTTTGCAACCGTTGACGAGGAAAATAATTTTGTCAATTCGATGTTTGTTAACACAGATTTTGTAACTAGTGAAAACAGAAGAATTTGA

Protein sequence

>MS.gene066274.t1

MYSTHSVIRSDDSEWFFLCPIEYKYAKSNKLKRATKFGFWKATGKDHSIKNRGTDEVIGTKKTLVYYLAPHPGVKTNWVIREYHDVVSVRFDDNTQRTFVLCRLMKKAEIKGEEENDTMMCDEGEPSRQMTASFPPILLVPPVGFRFCPTDEELISHYLRNKLLGNDAIVNNVIAEVDIRKFEPWELPARSVIKSDDPEWFFVCPLKYKYAKSKRINRTTKNGFWKATGMDRNIRIRGTDKVIGIKKTLVYHENPVPAVKTNWIIHEYHDATLEDHQRTFVLCRLIKKAENKIEEEAGMIICDEGESSRHMSSDYENQETAEGISDVSGTLPEINMESIFEEPFQADRYFPYSTQQSSLNENEPELLFSNSQFYNAYLKNENNVIQSMFATVDEENNFVNSMFVNTDFVTSENRRI