AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066293

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066293.t1 MTR_5g038280 95.195 333 7 1 1 333 1 324 0.0 644
MS.gene066293.t1 MTR_8g469430 53.030 330 94 8 10 333 12 286 2.90e-97 289
MS.gene066293.t1 MTR_4g107650 47.578 351 150 10 1 333 1 335 1.89e-79 246
MS.gene066293.t1 MTR_6g007647 47.208 197 90 3 87 280 33 218 1.20e-42 148
MS.gene066293.t1 MTR_6g007647 47.208 197 90 3 87 280 67 252 2.13e-42 149
MS.gene066293.t1 MTR_6g011610 43.388 242 95 10 39 261 41 259 1.23e-39 141
MS.gene066293.t1 MTR_6g011610 47.980 198 76 7 74 261 60 240 1.46e-39 140
MS.gene066293.t1 MTR_8g089895 64.815 108 37 1 77 183 43 150 7.39e-39 140
MS.gene066293.t1 MTR_3g086790 60.526 114 41 1 70 183 47 156 7.02e-38 137
MS.gene066293.t1 MTR_3g080100 66.327 98 33 0 87 184 20 117 2.35e-37 133
MS.gene066293.t1 MTR_3g080100 66.327 98 33 0 87 184 59 156 3.74e-37 134
MS.gene066293.t1 MTR_8g468210 66.667 96 32 0 87 182 77 172 8.05e-37 134
MS.gene066293.t1 MTR_5g039000 60.169 118 46 1 66 182 55 172 1.44e-36 133
MS.gene066293.t1 MTR_7g010020 58.559 111 44 1 74 182 65 175 3.90e-32 121
MS.gene066293.t1 MTR_1g061660 53.571 112 41 2 82 193 54 154 1.05e-26 105
MS.gene066293.t1 MTR_7g103340 55.670 97 36 1 87 183 63 152 1.21e-26 105
MS.gene066293.t1 MTR_3g092150 54.945 91 41 0 89 179 40 130 1.04e-25 103
MS.gene066293.t1 MTR_8g026960 47.573 103 53 1 91 193 38 139 1.75e-24 100
MS.gene066293.t1 MTR_5g019680 60.563 71 28 0 87 157 53 123 1.06e-19 86.3
MS.gene066293.t1 MTR_4g100550 45.902 122 53 4 67 186 103 213 3.23e-19 86.7
MS.gene066293.t1 MTR_5g019650 60.563 71 28 0 87 157 53 123 3.94e-19 85.1
MS.gene066293.t1 MTR_5g013010 40.645 155 71 5 37 185 89 228 1.42e-18 85.1
MS.gene066293.t1 MTR_5g014890 45.794 107 49 2 75 181 113 210 1.47e-17 81.6
MS.gene066293.t1 MTR_4g126900 46.154 91 42 1 90 180 91 174 1.95e-17 80.1
MS.gene066293.t1 MTR_8g006705 48.515 101 44 2 87 186 146 239 3.08e-17 81.3
MS.gene066293.t1 MTR_2g061030 44.340 106 52 1 75 180 188 286 4.33e-17 81.3
MS.gene066293.t1 MTR_7g093430 47.872 94 42 1 87 180 160 246 5.15e-17 80.5
MS.gene066293.t1 MTR_1g054285 46.809 94 43 1 87 180 154 240 3.19e-16 78.2
MS.gene066293.t1 MTR_4g097600 43.925 107 52 2 74 180 115 213 1.00e-15 76.3
MS.gene066293.t1 MTR_1g017090 46.316 95 44 1 87 181 130 217 1.82e-15 75.9
MS.gene066293.t1 MTR_4g084750 37.879 132 61 3 87 217 65 176 2.65e-15 73.9
MS.gene066293.t1 MTR_5g013010 41.667 108 50 3 37 139 89 188 6.84e-14 70.1
MS.gene066293.t1 MTR_3g103590 48.611 72 37 0 87 158 145 216 4.33e-13 69.3
MS.gene066293.t1 MTR_2g038625 41.489 94 48 1 87 180 82 168 2.77e-12 65.5
MS.gene066293.t1 MTR_2g038580 41.489 94 48 1 87 180 82 168 2.77e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066293.t1 AT3G01470 42.735 234 97 9 51 273 30 237 3.25e-42 147
MS.gene066293.t1 AT4G40060 54.615 130 53 2 76 202 45 171 6.00e-41 145
MS.gene066293.t1 AT2G22430 68.041 97 31 0 87 183 62 158 5.89e-39 140
MS.gene066293.t1 AT5G65310 68.041 97 31 0 87 183 54 150 2.47e-38 138
MS.gene066293.t1 AT5G65310 68.041 97 31 0 87 183 72 168 2.71e-38 138
MS.gene066293.t1 AT1G69780 64.545 110 38 1 74 182 71 180 2.74e-38 138
MS.gene066293.t1 AT3G01220 41.071 224 113 6 50 261 46 262 3.88e-37 135
MS.gene066293.t1 AT5G15150 60.000 115 44 1 66 180 96 208 2.28e-36 133
MS.gene066293.t1 AT1G26960 59.091 110 44 1 74 182 57 166 1.19e-33 125
MS.gene066293.t1 AT1G27045 46.939 147 60 3 37 183 36 164 1.11e-32 121
MS.gene066293.t1 AT1G27045 60.825 97 38 0 87 183 25 121 1.11e-31 117
MS.gene066293.t1 AT1G27045 60.825 97 38 0 87 183 25 121 1.11e-31 117
MS.gene066293.t1 AT1G27045 60.825 97 38 0 87 183 17 113 1.49e-31 117
MS.gene066293.t1 AT1G27045 46.259 147 60 4 37 183 36 163 1.97e-30 116
MS.gene066293.t1 AT2G46680 49.541 109 55 0 83 191 28 136 1.42e-28 111
MS.gene066293.t1 AT3G61890 51.429 105 50 1 90 194 33 136 1.79e-27 108
MS.gene066293.t1 AT2G46680 53.763 93 43 0 83 175 28 120 8.50e-27 106
MS.gene066293.t1 AT5G03790 53.608 97 38 1 87 183 77 166 2.08e-25 102
MS.gene066293.t1 AT5G03790 54.839 93 35 1 87 179 77 162 2.27e-24 98.6
MS.gene066293.t1 AT2G36610 47.222 108 42 2 84 183 68 168 7.92e-24 97.1
MS.gene066293.t1 AT4G36740 48.182 110 50 1 87 196 55 157 1.54e-22 94.4
MS.gene066293.t1 AT4G36740 48.182 110 50 1 87 196 56 158 1.66e-22 94.4
MS.gene066293.t1 AT2G18550 45.378 119 58 1 87 205 61 172 2.04e-22 94.0
MS.gene066293.t1 AT5G53980 46.392 97 52 0 87 183 11 107 6.85e-21 88.2
MS.gene066293.t1 AT5G66700 58.333 72 30 0 87 158 71 142 4.59e-20 87.8
MS.gene066293.t1 AT5G06710 48.421 95 42 1 87 181 190 277 3.71e-17 81.6
MS.gene066293.t1 AT4G17460 45.217 115 49 4 75 186 125 228 9.86e-17 79.7
MS.gene066293.t1 AT2G01430 39.706 136 64 4 50 180 103 225 1.98e-16 78.6
MS.gene066293.t1 AT5G47370 39.695 131 64 3 57 186 107 223 2.67e-16 78.2
MS.gene066293.t1 AT2G44910 47.000 100 45 2 87 185 163 255 4.06e-16 78.2
MS.gene066293.t1 AT3G60390 47.000 100 45 2 87 185 162 254 7.07e-16 77.4
MS.gene066293.t1 AT2G22800 45.263 95 45 1 87 181 113 200 9.17e-16 76.6
MS.gene066293.t1 AT4G37790 44.681 94 45 1 87 180 126 212 1.88e-15 75.9
MS.gene066293.t1 AT4G16780 46.000 100 46 2 87 185 129 221 2.57e-15 75.5
MS.gene066293.t1 AT5G06710 58.491 53 22 0 87 139 190 242 1.49e-12 67.4
MS.gene066293.t1 AT1G70920 43.299 97 48 1 84 180 66 155 1.80e-12 65.9
MS.gene066293.t1 AT1G70920 43.299 97 48 1 84 180 36 125 2.37e-12 65.1
MS.gene066293.t1 AT2G01430 41.053 95 45 3 50 139 103 191 3.76e-12 65.1

Find 49 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGAAAATTCTTGTAATTT+TGG 0.015177 5.3:-26476796 MS.gene066293:CDS
TCAATTACAAATTCTGAAAA+TGG 0.158542 5.3:-26477087 MS.gene066293:CDS
CTGAAGCCCTTGAATCTCTT+TGG 0.177337 5.3:-26478466 MS.gene066293:CDS
TGAACCTTGCCCTTCTGTTT+TGG 0.204900 5.3:+26477743 None:intergenic
TTCTCTTTCTCTTTATCTCT+TGG 0.249115 5.3:+26477180 None:intergenic
GCAAGTTGAACTTTCCTATC+AGG 0.270065 5.3:+26477808 None:intergenic
TAGCAAACATCTTCAACTTT+TGG 0.273698 5.3:+26476829 None:intergenic
TGAAGCCCTTGAATCTCTTT+GGG 0.300479 5.3:-26478465 MS.gene066293:CDS
TGAGGTGAATTGATTGCTTC+TGG 0.314138 5.3:+26477138 None:intergenic
CAAAGTGTCACTCCCTAATA+TGG 0.317058 5.3:-26477061 MS.gene066293:CDS
AACCAAGGCAAGTTGCTATA+TGG 0.341312 5.3:-26477768 MS.gene066293:CDS
GTGGAAATTTAAATTCAATA+AGG 0.341810 5.3:+26476739 None:intergenic
TCTCTTTCTCTTTATCTCTT+GGG 0.342274 5.3:+26477181 None:intergenic
CAAAAGGTTTGATCTTCAAC+AGG 0.362492 5.3:+26476769 None:intergenic
TTTCTCAAGTTGTTGAAAGA+AGG 0.381022 5.3:+26477954 None:intergenic
GATAGCCCACATTGCAATGA+TGG 0.423802 5.3:-26476982 MS.gene066293:CDS
ATTGGATCTTTGTTGTTATG+AGG 0.430991 5.3:+26477120 None:intergenic
TTCTCAAGTTGTTGAAAGAA+GGG 0.453337 5.3:+26477955 None:intergenic
GTAATTGAGATTATATCCAT+TGG 0.466626 5.3:+26477102 None:intergenic
CATATTAGGGAGTGACACTT+TGG 0.480614 5.3:+26477062 None:intergenic
GTTCAACTTGCAAAAGAGCT+TGG 0.481261 5.3:-26477796 MS.gene066293:CDS
AACCATATAGCAACTTGCCT+TGG 0.481745 5.3:+26477766 None:intergenic
CAATTACAAATTCTGAAAAT+GGG 0.483061 5.3:-26477086 MS.gene066293:CDS
TGTGAGAAATCTGAATGATC+AGG 0.485138 5.3:+26476907 None:intergenic
GAATGACAAGTTAAAAGAAG+AGG 0.495747 5.3:-26477635 MS.gene066293:intron
TATAAGGCTGTTCTTATGGC+AGG 0.496797 5.3:-26478554 None:intergenic
TCAATAAGGCCAGAAACAAA+AGG 0.504416 5.3:+26476753 None:intergenic
AAAACAAGCTTGAACCTGAT+AGG 0.509669 5.3:-26477822 MS.gene066293:CDS
AAGAGCTTGGTTTGCAACCA+AGG 0.513381 5.3:-26477783 MS.gene066293:CDS
TGTGAACCCAAAGAGATTCA+AGG 0.525903 5.3:+26478459 None:intergenic
AGATTTCTCACAAGATGAAG+AGG 0.546898 5.3:-26476896 MS.gene066293:CDS
TGTTCCCATCATTGCAATGT+GGG 0.548653 5.3:+26476977 None:intergenic
TCATAACAACAAAGATCCAA+TGG 0.563990 5.3:-26477118 MS.gene066293:CDS
GGATTCAGGTTCAAATTCAG+TGG 0.566281 5.3:-26478025 MS.gene066293:intron
TCAAGGGCTTCAGAAGTACA+AGG 0.569376 5.3:+26478476 None:intergenic
GTGAACCCAAAGAGATTCAA+GGG 0.581702 5.3:+26478460 None:intergenic
CTATATGGTTCCAAAACAGA+AGG 0.583686 5.3:-26477753 MS.gene066293:CDS
ATAGCCCACATTGCAATGAT+GGG 0.583931 5.3:-26476981 MS.gene066293:CDS
CTTGAGAAAGAAGAGAATTG+TGG 0.599550 5.3:-26477940 MS.gene066293:CDS
AAAGCATGTGAAGAATCTGT+AGG 0.619583 5.3:+26476934 None:intergenic
ATCACCTTGAAAAGAAGCAG+AGG 0.635119 5.3:+26478436 None:intergenic
TTGTTCCCATCATTGCAATG+TGG 0.645404 5.3:+26476976 None:intergenic
AAGGCTGTTCTTATGGCAGG+TGG 0.647915 5.3:-26478551 None:intergenic
GGTTCCAAAACAGAAGGGCA+AGG 0.651221 5.3:-26477747 MS.gene066293:CDS
TATATGGTTCCAAAACAGAA+GGG 0.680215 5.3:-26477752 MS.gene066293:CDS
AAAAGGTTTGATCTTCAACA+GGG 0.685076 5.3:+26476770 None:intergenic
GAAGCATGCTACCATCAACA+AGG 0.695864 5.3:-26477904 MS.gene066293:CDS
CAAGGGCTTCAGAAGTACAA+GGG 0.720149 5.3:+26478477 None:intergenic
TGGTGGTGGAAGCAACAGAG+TGG 0.722441 5.3:-26477989 MS.gene066293:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTATTTTGTTACAAT+AGG - chr5.3:26478063-26478082 MS.gene066293:intron 10.0%
!!! TAAAAAAGACTATTTTTTTA+TGG + chr5.3:26477159-26477178 None:intergenic 10.0%
!! TGAAAATTTACATTAGAATT+TGG + chr5.3:26477797-26477816 None:intergenic 15.0%
!!! ATGAAAATTCTTGTAATTTT+GGG - chr5.3:26478497-26478516 MS.gene066293:CDS 15.0%
!!! TGAAAAAGACTAATTTTATA+TGG + chr5.3:26477194-26477213 None:intergenic 15.0%
!! AATGAGTTAATGATTGAATA+TGG - chr5.3:26478023-26478042 MS.gene066293:intron 20.0%
!! ATGAGTTAATGATTGAATAT+GGG - chr5.3:26478024-26478043 MS.gene066293:intron 20.0%
!! CAAACTTTATTAAAGTAAAG+AGG + chr5.3:26477989-26478008 None:intergenic 20.0%
!! CATATAGTTGAATTATAACA+TGG - chr5.3:26477227-26477246 MS.gene066293:intron 20.0%
!!! AAATTACAAGAATTTTCATG+TGG + chr5.3:26478496-26478515 None:intergenic 20.0%
!!! AATTACAAGAATTTTCATGT+GGG + chr5.3:26478495-26478514 None:intergenic 20.0%
!!! CAATTACAAATTCTGAAAAT+GGG - chr5.3:26478206-26478225 MS.gene066293:intron 20.0%
!!! CATGAAAATTCTTGTAATTT+TGG - chr5.3:26478496-26478515 MS.gene066293:CDS 20.0%
!!! TACATTTTAGTACCATATTA+GGG + chr5.3:26478246-26478265 None:intergenic 20.0%
!!! TCAATTACAAATTCTGAAAA+TGG - chr5.3:26478205-26478224 MS.gene066293:intron 20.0%
!!! TTACATTTTAGTACCATATT+AGG + chr5.3:26478247-26478266 None:intergenic 20.0%
! AAACAGCTTGAAAAAGATTA+TGG - chr5.3:26477577-26477596 MS.gene066293:intron 25.0%
! CAATAGAAAGTTTGTATTGA+CGG + chr5.3:26477030-26477049 None:intergenic 25.0%
! CAATTCAAATATACACATGA+TGG + chr5.3:26476952-26476971 None:intergenic 25.0%
! CATGTGTATATTTGAATTGA+CGG - chr5.3:26476953-26476972 MS.gene066293:CDS 25.0%
! GTAATTGAGATTATATCCAT+TGG + chr5.3:26478193-26478212 None:intergenic 25.0%
! TCAATCATTAACTCATTAGT+GGG + chr5.3:26478020-26478039 None:intergenic 25.0%
! TGAGTTAATGATTGAATATG+GGG - chr5.3:26478025-26478044 MS.gene066293:intron 25.0%
! TTCAATCATTAACTCATTAG+TGG + chr5.3:26478021-26478040 None:intergenic 25.0%
! TTTCTTAAGATCATGAAAGT+GGG - chr5.3:26477110-26477129 MS.gene066293:CDS 25.0%
!! ATTTCTTGAAAAGAGTTTTG+AGG - chr5.3:26477444-26477463 MS.gene066293:intron 25.0%
AAAAGAAGTGAGAAGAATTG+AGG + chr5.3:26476890-26476909 None:intergenic 30.0%
AAAAGGTTTGATCTTCAACA+GGG + chr5.3:26478525-26478544 None:intergenic 30.0%
ACCATCAACAAGGAAAAAAA+AGG - chr5.3:26477398-26477417 MS.gene066293:intron 30.0%
ATCAACAAGGAAAAAAAAGG+AGG - chr5.3:26477401-26477420 MS.gene066293:intron 30.0%
CGTCAATACAAACTTTCTAT+TGG - chr5.3:26477028-26477047 MS.gene066293:CDS 30.0%
GAATGACAAGTTAAAAGAAG+AGG - chr5.3:26477657-26477676 MS.gene066293:CDS 30.0%
GTCAACACATCAATAAATGT+TGG - chr5.3:26477755-26477774 MS.gene066293:CDS 30.0%
GTTTCTTAAGATCATGAAAG+TGG - chr5.3:26477109-26477128 MS.gene066293:CDS 30.0%
TATATGGTTCCAAAACAGAA+GGG - chr5.3:26477540-26477559 MS.gene066293:intron 30.0%
TCATAACAACAAAGATCCAA+TGG - chr5.3:26478174-26478193 MS.gene066293:intron 30.0%
TCTCTTTCTCTTTATCTCTT+GGG + chr5.3:26478114-26478133 None:intergenic 30.0%
TTCTCTTTCTCTTTATCTCT+TGG + chr5.3:26478115-26478134 None:intergenic 30.0%
TTGACACACACAAATGAAAT+TGG - chr5.3:26477696-26477715 MS.gene066293:CDS 30.0%
! TAGCAAACATCTTCAACTTT+TGG + chr5.3:26478466-26478485 None:intergenic 30.0%
! TTCTCAAGTTGTTGAAAGAA+GGG + chr5.3:26477340-26477359 None:intergenic 30.0%
! TTTCTCAAGTTGTTGAAAGA+AGG + chr5.3:26477341-26477360 None:intergenic 30.0%
!! AAGATCAAACCTTTTGTTTC+TGG - chr5.3:26478530-26478549 MS.gene066293:CDS 30.0%
!! ATTGGATCTTTGTTGTTATG+AGG + chr5.3:26478175-26478194 None:intergenic 30.0%
!! TCAGTGGTTAATTTTGAGAA+TGG - chr5.3:26477283-26477302 MS.gene066293:intron 30.0%
!!! ATGTGTATGTGTGTTTTGAT+AGG + chr5.3:26477924-26477943 None:intergenic 30.0%
!!! TCCTTTTTTTTCCTTGTTGA+TGG + chr5.3:26477402-26477421 None:intergenic 30.0%
AAAACAAGCTTGAACCTGAT+AGG - chr5.3:26477470-26477489 MS.gene066293:intron 35.0%
AAAGCATGTGAAGAATCTGT+AGG + chr5.3:26478361-26478380 None:intergenic 35.0%
AATTGTCTGATCTCAATCCA+AGG - chr5.3:26477849-26477868 MS.gene066293:CDS 35.0%
AGATTTCTCACAAGATGAAG+AGG - chr5.3:26478396-26478415 MS.gene066293:intron 35.0%
CAAAAGGTTTGATCTTCAAC+AGG + chr5.3:26478526-26478545 None:intergenic 35.0%
CTATATGGTTCCAAAACAGA+AGG - chr5.3:26477539-26477558 MS.gene066293:intron 35.0%
CTTGAGAAAGAAGAGAATTG+TGG - chr5.3:26477352-26477371 MS.gene066293:intron 35.0%
GTTAGTAAATCGATGATCCT+TGG + chr5.3:26477869-26477888 None:intergenic 35.0%
TCAATAAGGCCAGAAACAAA+AGG + chr5.3:26478542-26478561 None:intergenic 35.0%
TGTGAGAAATCTGAATGATC+AGG + chr5.3:26478388-26478407 None:intergenic 35.0%
! ATGGTTTGTGTTTGATGACA+TGG - chr5.3:26477246-26477265 MS.gene066293:intron 35.0%
! TCTTCAACTTTTGGTAAGCA+AGG + chr5.3:26478457-26478476 None:intergenic 35.0%
!! CACATCACTTTTAGCTGAAT+TGG + chr5.3:26478281-26478300 None:intergenic 35.0%
!! GTGGTTAATTTTGAGAATGG+TGG - chr5.3:26477286-26477305 MS.gene066293:intron 35.0%
!! GTTAATTTTGAGAATGGTGG+TGG - chr5.3:26477289-26477308 MS.gene066293:intron 35.0%
AACCAAGGCAAGTTGCTATA+TGG - chr5.3:26477524-26477543 MS.gene066293:intron 40.0%
AACCATATAGCAACTTGCCT+TGG + chr5.3:26477529-26477548 None:intergenic 40.0%
AGAAGTGAGAAGAATTGAGG+AGG + chr5.3:26476887-26476906 None:intergenic 40.0%
ATAGCCCACATTGCAATGAT+GGG - chr5.3:26478311-26478330 MS.gene066293:intron 40.0%
ATCACCTTGAAAAGAAGCAG+AGG + chr5.3:26476859-26476878 None:intergenic 40.0%
CATATTAGGGAGTGACACTT+TGG + chr5.3:26478233-26478252 None:intergenic 40.0%
GTGAACCCAAAGAGATTCAA+GGG + chr5.3:26476835-26476854 None:intergenic 40.0%
GTTCAACTTGCAAAAGAGCT+TGG - chr5.3:26477496-26477515 MS.gene066293:intron 40.0%
TGAAGCCCTTGAATCTCTTT+GGG - chr5.3:26476827-26476846 MS.gene066293:CDS 40.0%
TGAGGTGAATTGATTGCTTC+TGG + chr5.3:26478157-26478176 None:intergenic 40.0%
TGTGAACCCAAAGAGATTCA+AGG + chr5.3:26476836-26476855 None:intergenic 40.0%
TGTTCCCATCATTGCAATGT+GGG + chr5.3:26478318-26478337 None:intergenic 40.0%
TTGTTCCCATCATTGCAATG+TGG + chr5.3:26478319-26478338 None:intergenic 40.0%
! CAAAGTGTCACTCCCTAATA+TGG - chr5.3:26478231-26478250 MS.gene066293:intron 40.0%
! GCAAGTTGAACTTTCCTATC+AGG + chr5.3:26477487-26477506 None:intergenic 40.0%
! GGATTCAGGTTCAAATTCAG+TGG - chr5.3:26477267-26477286 MS.gene066293:intron 40.0%
! GTGTTTGATGACATGGATTC+AGG - chr5.3:26477253-26477272 MS.gene066293:intron 40.0%
!!! TGTGTGTTTTGATAGGTGAC+CGG + chr5.3:26477917-26477936 None:intergenic 40.0%
CTGAAGCCCTTGAATCTCTT+TGG - chr5.3:26476826-26476845 MS.gene066293:CDS 45.0%
GAAGCATGCTACCATCAACA+AGG - chr5.3:26477388-26477407 MS.gene066293:intron 45.0%
GATAGCCCACATTGCAATGA+TGG - chr5.3:26478310-26478329 MS.gene066293:intron 45.0%
TTTGATAGGTGACCGGTGTT+CGG + chr5.3:26477910-26477929 None:intergenic 45.0%
! CAAGGGCTTCAGAAGTACAA+GGG + chr5.3:26476818-26476837 None:intergenic 45.0%
! CACACCTCTGCTTCTTTTCA+AGG - chr5.3:26476852-26476871 MS.gene066293:CDS 45.0%
! TCAAGGGCTTCAGAAGTACA+AGG + chr5.3:26476819-26476838 None:intergenic 45.0%
! TGAACCTTGCCCTTCTGTTT+TGG + chr5.3:26477552-26477571 None:intergenic 45.0%
!! AAGAGCTTGGTTTGCAACCA+AGG - chr5.3:26477509-26477528 MS.gene066293:intron 45.0%
GGTTCCAAAACAGAAGGGCA+AGG - chr5.3:26477545-26477564 MS.gene066293:intron 50.0%
! ATGGCAGGTGGCAAGCTTTT+TGG - chr5.3:26476753-26476772 MS.gene066293:CDS 50.0%
CATTCCGAGAGTCCGAACAC+CGG - chr5.3:26477895-26477914 MS.gene066293:CDS 55.0%
! GCAGGTGGCAAGCTTTTTGG+TGG - chr5.3:26476756-26476775 MS.gene066293:CDS 55.0%
!! TGGTGGTGGAAGCAACAGAG+TGG - chr5.3:26477303-26477322 MS.gene066293:intron 55.0%
GTGACCGGTGTTCGGACTCT+CGG + chr5.3:26477902-26477921 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 26476753 26478561 26476753 ID=MS.gene066293
chr5.3 mRNA 26476753 26478561 26476753 ID=MS.gene066293.t1;Parent=MS.gene066293
chr5.3 exon 26478441 26478561 26478441 ID=MS.gene066293.t1.exon1;Parent=MS.gene066293.t1
chr5.3 CDS 26478441 26478561 26478441 ID=cds.MS.gene066293.t1;Parent=MS.gene066293.t1
chr5.3 exon 26477636 26478039 26477636 ID=MS.gene066293.t1.exon2;Parent=MS.gene066293.t1
chr5.3 CDS 26477636 26478039 26477636 ID=cds.MS.gene066293.t1;Parent=MS.gene066293.t1
chr5.3 exon 26476753 26477229 26476753 ID=MS.gene066293.t1.exon3;Parent=MS.gene066293.t1
chr5.3 CDS 26476753 26477229 26476753 ID=cds.MS.gene066293.t1;Parent=MS.gene066293.t1
Gene Sequence

>MS.gene066293

ATGGCAGGTGGCAAGCTTTTTGGTGGTTCTAATATGTCACTTTTGCTTCAAAATGAAAGACTCCCTTGTACTTCTGAAGCCCTTGAATCTCTTTGGGTTCACACCTCTGCTTCTTTTCAAGGTGATCTTCACCTCCTCAATTCTTCTCACTTCTTTTGTTACATAATTGTCATCAGTTTTTGTAGTTGTTTCTTCACCATCATGTGTATATTTGAATTGACGGTGAGTTAGACAGAATCACTGATACACATAATCACTGATTCTGTCTAACTTACCGTCAATACAAACTTTCTATTGGTCTTAAAATCTCACAATTTTAAGTTTTATGAATATCATAGAGCATAACACACACATATGTTTCTTAAGATCATGAAAGTGGGTTTCTTTTTTCATTGTGAAGAAACCATAAAAAAATAGTCTTTTTTATTTTTGAAGAAACCATATAAAATTAGTCTTTTTCATTATTTTTCTATGCATATAGTTGAATTATAACATGGTTTGTGTTTGATGACATGGATTCAGGTTCAAATTCAGTGGTTAATTTTGAGAATGGTGGTGGAAGCAACAGAGTGGTAACAGATAGACCCTTCTTTCAACAACTTGAGAAAGAAGAGAATTGTGGTGATGAAGATTATGAAGCATGCTACCATCAACAAGGAAAAAAAAGGAGGCTTTCAAGTGAACAAGTTCAATTTCTTGAAAAGAGTTTTGAGGTAGAAAACAAGCTTGAACCTGATAGGAAAGTTCAACTTGCAAAAGAGCTTGGTTTGCAACCAAGGCAAGTTGCTATATGGTTCCAAAACAGAAGGGCAAGGTTCAAAACTAAACAGCTTGAAAAAGATTATGGCACATTGAAAGCTAGCTTTGATAGTCTCAAAGATGATTATGATAATCTTCTTCAAGAGAATGACAAGTTAAAAGAAGAGGTAAAAAAAGTAAAAAGTTGACACACACAAATGAAATTGGACACTATGTTGTCATTGATCGCACGCATTTCACACAGTCAACACATCAATAAATGTTGGATCGAGATCAGACAATCCAAATTCTAATGTAAATTTTCATCTTAAATCTAAAGTATCAAACTTAAAATTTGAATTGTCTGATCTCAATCCAAGGATCATCGATTTACTAACAGCGTGCATTCCGAGAGTCCGAACACCGGTCACCTATCAAAACACACATACACATAAAAACATACTAATTCAGCTTTTCTACTTTATGCTGCTATGACCTCTTTACTTTAATAAAGTTTGATATTTCTCCCACTAATGAGTTAATGATTGAATATGGGGTTAATTTTATTTTTTATTTTTTATTTTGTTACAATAGGTTAATTCTCTCAAGAACAAATTGATCCCAAGAGATAAAGAGAAAGAGAATTCAGATGAAAAATCATCACCAGAAGCAATCAATTCACCTCATAACAACAAAGATCCAATGGATATAATCTCAATTACAAATTCTGAAAATGGGTCCAAAGTGTCACTCCCTAATATGGTACTAAAATGTAAGCAAGAAGATGCCAATTCAGCTAAAAGTGATGTGCTTGATTCTGATAGCCCACATTGCAATGATGGGAACAATCTTTCTTCTTTCATAGAGCCTACAGATTCTTCACATGCTTTTGAACCTGATCATTCAGATTTCTCACAAGATGAAGAGGATAATGATAACTTGAGTCATAATCTTTTGACTCTTCCTTGCTTACCAAAAGTTGAAGATGTTTGCTATGATGACCCACATGAAAATTCTTGTAATTTTGGGTTCCCTGTTGAAGATCAAACCTTTTGTTTCTGGCCTTATTGA
Protein sequence

>MS.gene066293.t1

MAGGKLFGGSNMSLLLQNERLPCTSEALESLWVHTSASFQGSNSVVNFENGGGSNRVVTDRPFFQQLEKEENCGDEDYEACYHQQGKKRRLSSEQVQFLEKSFEVENKLEPDRKVQLAKELGLQPRQVAIWFQNRRARFKTKQLEKDYGTLKASFDSLKDDYDNLLQENDKLKEEVNSLKNKLIPRDKEKENSDEKSSPEAINSPHNNKDPMDIISITNSENGSKVSLPNMVLKCKQEDANSAKSDVLDSDSPHCNDGNNLSSFIEPTDSSHAFEPDHSDFSQDEEDNDNLSHNLLTLPCLPKVEDVCYDDPHENSCNFGFPVEDQTFCFWPY