AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066341


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066341.t1 MTR_4g120380 70.213 282 82 1 1 282 94 373 1.66e-142 405
MS.gene066341.t1 MTR_4g119580 68.929 280 85 1 1 280 94 371 9.74e-128 368
MS.gene066341.t1 MTR_4g120380 69.112 259 78 1 1 259 94 350 2.28e-125 363
MS.gene066341.t1 MTR_4g119570 59.216 255 69 2 28 282 13 232 2.26e-97 286
MS.gene066341.t1 MTR_2g437040 46.953 279 146 1 1 279 92 368 3.63e-87 265
MS.gene066341.t1 MTR_2g436400 46.809 282 148 1 1 282 99 378 1.79e-85 261
MS.gene066341.t1 MTR_2g436460 48.387 279 141 2 1 278 97 373 9.36e-85 259
MS.gene066341.t1 MTR_4g119550 46.429 280 147 2 1 279 101 378 8.38e-84 257
MS.gene066341.t1 MTR_2g437030 49.275 276 138 1 4 279 181 454 1.13e-81 254
MS.gene066341.t1 MTR_2g437990 46.786 280 145 2 1 278 102 379 1.96e-81 252
MS.gene066341.t1 MTR_2g438010 44.681 282 151 2 1 279 122 401 9.95e-80 247
MS.gene066341.t1 MTR_2g437960 45.390 282 149 2 1 279 105 384 2.41e-79 246
MS.gene066341.t1 MTR_2g437180 45.907 281 145 3 1 278 101 377 4.70e-79 244
MS.gene066341.t1 MTR_2g437150 46.479 284 145 3 1 279 107 388 5.48e-79 245
MS.gene066341.t1 MTR_2g437960 45.390 282 149 2 1 279 105 384 6.19e-79 245
MS.gene066341.t1 MTR_2g076320 44.840 281 150 2 1 278 102 380 1.28e-78 244
MS.gene066341.t1 MTR_2g437170 45.775 284 147 3 1 279 107 388 1.49e-77 241
MS.gene066341.t1 MTR_2g437020 45.520 279 151 1 1 279 91 368 1.57e-77 240
MS.gene066341.t1 MTR_2g437130 44.444 279 153 1 1 279 93 369 1.64e-77 240
MS.gene066341.t1 MTR_2g437200 42.271 317 143 3 1 279 82 396 2.52e-77 240
MS.gene066341.t1 MTR_2g437120 45.455 286 142 5 1 279 103 381 6.47e-77 240
MS.gene066341.t1 MTR_2g437100 46.127 284 146 3 1 279 112 393 9.60e-77 239
MS.gene066341.t1 MTR_2g437260 42.294 279 161 0 1 279 86 364 1.34e-76 238
MS.gene066341.t1 MTR_2g437060 43.369 279 156 1 1 279 107 383 4.97e-76 237
MS.gene066341.t1 MTR_2g437160 45.552 281 149 3 1 279 115 393 1.09e-74 234
MS.gene066341.t1 MTR_2g437080 44.444 279 153 1 1 279 92 368 1.79e-74 233
MS.gene066341.t1 MTR_2g437240 40.379 317 149 3 1 279 82 396 1.59e-73 231
MS.gene066341.t1 MTR_2g436440 44.203 276 152 1 4 279 101 374 8.21e-73 228
MS.gene066341.t1 MTR_2g438020 40.694 317 148 3 1 279 121 435 2.48e-71 228
MS.gene066341.t1 MTR_2g019810 43.357 286 146 4 1 279 81 357 3.16e-70 221
MS.gene066341.t1 MTR_2g019840 42.294 279 159 2 1 279 93 369 1.75e-68 217
MS.gene066341.t1 MTR_2g437940 41.935 279 135 4 1 279 101 352 4.60e-64 206
MS.gene066341.t1 MTR_2g437090 52.597 154 73 0 126 279 3 156 4.53e-51 166
MS.gene066341.t1 MTR_2g437160 49.123 171 85 1 109 279 134 302 7.41e-50 167
MS.gene066341.t1 MTR_3g450500 32.028 281 185 2 5 280 92 371 7.10e-46 158
MS.gene066341.t1 MTR_4g007490 31.338 284 188 3 1 279 87 368 1.21e-42 150
MS.gene066341.t1 MTR_8g080990 26.690 281 205 1 1 280 93 373 3.02e-30 117
MS.gene066341.t1 MTR_8g081000 25.704 284 208 3 1 282 106 388 5.27e-27 108
MS.gene066341.t1 MTR_8g081000 25.704 284 208 3 1 282 106 388 5.56e-27 108
MS.gene066341.t1 MTR_8g081000 25.704 284 208 3 1 282 106 388 5.73e-27 108
MS.gene066341.t1 MTR_4g119615 74.510 51 13 0 223 273 26 76 4.41e-17 75.1
MS.gene066341.t1 MTR_2g436370 36.735 98 62 0 1 98 98 195 5.71e-16 75.1
MS.gene066341.t1 MTR_2g436380 50.820 61 30 0 126 186 3 63 6.88e-13 67.8
MS.gene066341.t1 MTR_2g019830 53.968 63 28 1 217 279 136 197 1.34e-11 62.8
MS.gene066341.t1 MTR_3g085240 35.955 89 40 1 183 271 176 247 2.79e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066341.t1 AT5G07900 30.282 284 194 2 1 280 118 401 1.92e-46 160
MS.gene066341.t1 AT1G61990 29.283 321 177 8 1 281 101 411 2.93e-30 117
MS.gene066341.t1 AT1G61990 29.283 321 177 8 1 281 101 411 2.93e-30 117
MS.gene066341.t1 AT1G21150 26.335 281 201 3 1 276 69 348 3.47e-28 111
MS.gene066341.t1 AT1G61980 25.392 319 197 7 1 280 101 417 4.90e-28 112
MS.gene066341.t1 AT1G61980 25.392 319 197 7 1 280 101 417 4.90e-28 112
MS.gene066341.t1 AT1G61980 25.392 319 197 7 1 280 101 417 4.90e-28 112
MS.gene066341.t1 AT1G61970 25.938 320 194 9 1 280 101 417 5.20e-28 111
MS.gene066341.t1 AT1G61970 25.938 320 194 9 1 280 101 417 5.20e-28 111
MS.gene066341.t1 AT1G61970 25.938 320 194 9 1 280 101 417 5.20e-28 111
MS.gene066341.t1 AT1G61970 25.938 320 194 9 1 280 101 417 5.20e-28 111
MS.gene066341.t1 AT1G61970 25.938 320 194 9 1 280 101 417 5.20e-28 111
MS.gene066341.t1 AT1G61970 25.938 320 194 9 1 280 101 417 5.20e-28 111
MS.gene066341.t1 AT1G61970 25.938 320 194 9 1 280 101 417 5.20e-28 111
MS.gene066341.t1 AT1G21150 26.335 281 201 3 1 276 105 384 5.60e-28 111
MS.gene066341.t1 AT5G64950 29.110 292 197 3 1 282 88 379 8.26e-28 110
MS.gene066341.t1 AT1G21150 26.335 281 201 3 1 276 169 448 9.47e-28 111
MS.gene066341.t1 AT1G62120 24.062 320 202 6 1 280 105 423 5.74e-25 103
MS.gene066341.t1 AT1G62010 23.913 322 198 7 1 281 87 402 3.80e-23 98.2
MS.gene066341.t1 AT1G62150 23.032 343 190 6 1 270 107 448 3.47e-21 93.2
MS.gene066341.t1 AT3G46950 21.264 348 206 6 1 281 101 447 6.59e-18 83.6
MS.gene066341.t1 AT1G62085 29.744 195 134 2 1 193 104 297 7.71e-18 83.6
MS.gene066341.t1 AT1G62085 23.784 185 137 3 89 270 262 445 4.37e-11 63.5
MS.gene066341.t1 AT1G62085 29.744 195 134 2 1 193 104 297 7.71e-18 83.6
MS.gene066341.t1 AT1G62085 23.784 185 137 3 89 270 262 445 4.37e-11 63.5
MS.gene066341.t1 AT1G62110 22.284 359 200 8 1 281 101 458 7.20e-17 80.5
MS.gene066341.t1 AT1G61960 27.179 195 136 3 90 280 261 453 1.86e-16 79.3
MS.gene066341.t1 AT1G61960 26.531 196 140 3 1 194 101 294 4.01e-15 75.5
MS.gene066341.t1 AT1G56380 21.865 311 201 7 1 270 65 374 2.65e-15 75.5
MS.gene066341.t1 AT1G56380 21.865 311 201 7 1 270 76 385 3.11e-15 75.5
MS.gene066341.t1 AT5G23930 21.387 346 204 6 1 280 104 447 3.16e-14 72.8

Find 0 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATATGTGAATTATTTTG+AGG + 32540:7602-7621 MS.gene066341:CDS 20.0%
!!! AATAATTCACATATCTTTTC+AGG - 32540:7599-7618 None:intergenic 20.0%
! AAATTGAAACTTTGGCAAAA+TGG - 32540:6878-6897 None:intergenic 25.0%
! AAGCAAATAGTAAATACTTG+AGG - 32540:7130-7149 None:intergenic 25.0%
! AGCAAATAGTAAATACTTGA+GGG - 32540:7129-7148 None:intergenic 25.0%
! ATACTAAATCCGAAAATATC+TGG - 32540:7465-7484 None:intergenic 25.0%
! GAAGCTAAAAATTGAAACTT+TGG - 32540:6886-6905 None:intergenic 25.0%
! TATAGTAATAGCTCTTTGAT+CGG - 32540:7028-7047 None:intergenic 25.0%
! TCAAGCAAAAAATGAACATT+TGG - 32540:7093-7112 None:intergenic 25.0%
! TCAGATATACAGAATATAGA+AGG - 32540:7162-7181 None:intergenic 25.0%
!! GTATATCTGATTCTGATTTT+AGG + 32540:7172-7191 MS.gene066341:CDS 25.0%
!!! ATATGTGAATTATTTTGAGG+AGG + 32540:7605-7624 MS.gene066341:CDS 25.0%
!!! GTTCATTTTTTGCTTGATTT+TGG + 32540:7096-7115 MS.gene066341:CDS 25.0%
!!! TTTCAATTTTTAGCTTCCAA+AGG + 32540:6889-6908 MS.gene066341:CDS 25.0%
AACTCATCAGTCAAAGAAAA+TGG - 32540:7573-7592 None:intergenic 30.0%
ACACAAAAATATCCATTGGA+AGG - 32540:7234-7253 None:intergenic 30.0%
CACAAAAATATCCATTGGAA+GGG - 32540:7233-7252 None:intergenic 30.0%
TCGGATTTAGTATAGAGAAA+AGG + 32540:7472-7491 MS.gene066341:CDS 30.0%
TTCTTACCAAATCAAAAGCA+GGG - 32540:6993-7012 None:intergenic 30.0%
! TTGAAAGCTCCAGATATTTT+CGG + 32540:7453-7472 MS.gene066341:CDS 30.0%
!! AATAATCCCTGCTTTTGATT+TGG + 32540:6984-7003 MS.gene066341:CDS 30.0%
!! ATATTTTTGTGTGGCATTAC+GGG + 32540:7242-7261 MS.gene066341:CDS 30.0%
!! ATCTGATTCTGATTTTAGGA+AGG + 32540:7176-7195 MS.gene066341:CDS 30.0%
!! GATATTTTTGTGTGGCATTA+CGG + 32540:7241-7260 MS.gene066341:CDS 30.0%
!! TTCCAATGGATATTTTTGTG+TGG + 32540:7233-7252 MS.gene066341:CDS 30.0%
ACACTGTTAAGGCTATATCA+GGG + 32540:7636-7655 MS.gene066341:CDS 35.0%
ACTCTTCTTCATCAAACCTT+TGG - 32540:7535-7554 None:intergenic 35.0%
AGAGAAAAGGCTTATTCCAA+GGG + 32540:7485-7504 MS.gene066341:CDS 35.0%
CAGGGATTATTTGTTTGTCA+AGG - 32540:6975-6994 None:intergenic 35.0%
CTTCTTACCAAATCAAAAGC+AGG - 32540:6994-7013 None:intergenic 35.0%
TACACTGTTAAGGCTATATC+AGG + 32540:7635-7654 MS.gene066341:CDS 35.0%
TAGAGAAAAGGCTTATTCCA+AGG + 32540:7484-7503 MS.gene066341:CDS 35.0%
TGCCACACAAAAATATCCAT+TGG - 32540:7238-7257 None:intergenic 35.0%
TTTGATCCAAAAACGCATCA+CGG - 32540:7406-7425 None:intergenic 35.0%
TTTGGCAAAATGGTTTCGAT+GGG - 32540:6868-6887 None:intergenic 35.0%
! AACCTTTGGAAAGAAGATAC+TGG - 32540:7521-7540 None:intergenic 35.0%
! ATCAACTTTTGCATCCCAAT+GGG - 32540:7289-7308 None:intergenic 35.0%
! ATGGGATTTGAAACGATGAT+GGG - 32540:6850-6869 None:intergenic 35.0%
ACAGTGACAACTATGTCTTG+AGG - 32540:6922-6941 None:intergenic 40.0%
CGAGATCTAGCAATTGAAGT+TGG - 32540:7063-7082 None:intergenic 40.0%
CTTTGGCAAAATGGTTTCGA+TGG - 32540:6869-6888 None:intergenic 40.0%
GTAATAGCTCTTTGATCGGA+AGG - 32540:7024-7043 None:intergenic 40.0%
GTCCAGTATCTTCTTTCCAA+AGG + 32540:7516-7535 MS.gene066341:CDS 40.0%
GTTTGTCAAGGCTTGAACAA+AGG - 32540:6963-6982 None:intergenic 40.0%
TGGAGGAGATAAAGGGATTA+GGG + 32540:7202-7221 MS.gene066341:CDS 40.0%
TTGGATCAAACAGCATGGTT+GGG + 32540:7416-7435 MS.gene066341:CDS 40.0%
TTTGGATCAAACAGCATGGT+TGG + 32540:7415-7434 MS.gene066341:CDS 40.0%
! AGTTGATGCCTTCAAGAGTT+GGG + 32540:7302-7321 MS.gene066341:CDS 40.0%
! CATCAACTTTTGCATCCCAA+TGG - 32540:7290-7309 None:intergenic 40.0%
! GATGGGATTTGAAACGATGA+TGG - 32540:6851-6870 None:intergenic 40.0%
! TTTTGCATCCCAATGGGATT+TGG - 32540:7283-7302 None:intergenic 40.0%
!! AAGTTGATGCCTTCAAGAGT+TGG + 32540:7301-7320 MS.gene066341:CDS 40.0%
!! AGGAAGCATCTACACTGTTA+AGG + 32540:7625-7644 MS.gene066341:CDS 40.0%
!! CGTTTTTGGATCAAACAGCA+TGG + 32540:7411-7430 MS.gene066341:CDS 40.0%
!! GAAAGCCTAGCTTTATGCTA+CGG + 32540:7361-7380 MS.gene066341:CDS 40.0%
!! TAGCTTTATGCTACGGTCTA+GGG + 32540:7368-7387 MS.gene066341:CDS 40.0%
!! TTCTGATTTTAGGAAGGCTG+TGG + 32540:7182-7201 MS.gene066341:CDS 40.0%
AAGGCTGTGGAGGAGATAAA+GGG + 32540:7195-7214 MS.gene066341:CDS 45.0%
AGAACAACCCCAACTCTTGA+AGG - 32540:7313-7332 None:intergenic 45.0%
AGGCTTGAACAAAGGAAACG+AGG - 32540:6955-6974 None:intergenic 45.0%
CTAGACCGTAGCATAAAGCT+AGG - 32540:7369-7388 None:intergenic 45.0%
CTGTTAAGGCTATATCAGGG+AGG + 32540:7639-7658 MS.gene066341:CDS 45.0%
GGCTATCTCCAAATCCCATT+GGG + 32540:7272-7291 MS.gene066341:CDS 45.0%
GGCTTGAACAAAGGAAACGA+GGG - 32540:6954-6973 None:intergenic 45.0%
GTGGAGGAGATAAAGGGATT+AGG + 32540:7201-7220 MS.gene066341:CDS 45.0%
GTTGATGCCTTCAAGAGTTG+GGG + 32540:7303-7322 MS.gene066341:CDS 45.0%
TGGCTATCTCCAAATCCCAT+TGG + 32540:7271-7290 MS.gene066341:CDS 45.0%
TTAGGGTTTCACCCTTCCAA+TGG + 32540:7219-7238 MS.gene066341:CDS 45.0%
! TCAATGCCGTGATGCGTTTT+TGG + 32540:7397-7416 MS.gene066341:CDS 45.0%
! TGTGGCATTACGGGCTAAAA+TGG + 32540:7251-7270 MS.gene066341:CDS 45.0%
!! CTAGCTTTATGCTACGGTCT+AGG + 32540:7367-7386 MS.gene066341:CDS 45.0%
!! GGATTTGAAACGATGATGGG+TGG - 32540:6847-6866 None:intergenic 45.0%
!! TGATTTTAGGAAGGCTGTGG+AGG + 32540:7185-7204 MS.gene066341:CDS 45.0%
GAAGGCTGTGGAGGAGATAA+AGG + 32540:7194-7213 MS.gene066341:CDS 50.0%
TACTGGACAACAGAAGCCCT+TGG - 32540:7504-7523 None:intergenic 50.0%
! GGAGCTTTCAAGAGCACGAA+AGG - 32540:7444-7463 None:intergenic 50.0%
!! GTCTTGAGGAGAAGCACCTT+TGG - 32540:6908-6927 None:intergenic 50.0%
GGGTGGAATCCTGCGAATGA+TGG - 32540:6830-6849 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
32540 gene 6820 7668 6820 ID=MS.gene066341
32540 mRNA 6820 7668 6820 ID=MS.gene066341.t1;Parent=MS.gene066341
32540 exon 6820 7668 6820 ID=MS.gene066341.t1.exon1;Parent=MS.gene066341.t1
32540 CDS 6820 7668 6820 ID=cds.MS.gene066341.t1;Parent=MS.gene066341.t1
Gene Sequence

>MS.gene066341

ATACAAACCATCATTCGCAGGATTCCACCCATCATCGTTTCAAATCCCATCGAAACCATTTTGCCAAAGTTTCAATTTTTAGCTTCCAAAGGTGCTTCTCCTCAAGACATAGTTGTCACTGTCACTAAAAGCCCTCGTTTCCTTTGTTCAAGCCTTGACAAACAAATAATCCCTGCTTTTGATTTGGTAAGAAGTTTCTGTCCTTCCGATCAAAGAGCTATTACTATAATAAATTCTTATCCAACTTCAATTGCTAGATCTCGCATGAAACCAAATGTTCATTTTTTGCTTGATTTTGGAGTCAATCCCTCAAGTATTTACTATTTGCTTAGTAGTAGACCTTCTATATTCTGTATATCTGATTCTGATTTTAGGAAGGCTGTGGAGGAGATAAAGGGATTAGGGTTTCACCCTTCCAATGGATATTTTTGTGTGGCATTACGGGCTAAAATGGCTATCTCCAAATCCCATTGGGATGCAAAAGTTGATGCCTTCAAGAGTTGGGGTTGTTCTGAAGATGATATTTTGACTGCATTTAAAAGAAAGCCTAGCTTTATGCTACGGTCTAGGGACAAACTCAATGCCGTGATGCGTTTTTGGATCAAACAGCATGGTTGGGATCCTTTCGTGCTCTTGAAAGCTCCAGATATTTTCGGATTTAGTATAGAGAAAAGGCTTATTCCAAGGGCTTCTGTTGTCCAGTATCTTCTTTCCAAAGGTTTGATGAAGAAGAGTGCTAGCTTATATACACCATTTTCTTTGACTGATGAGTTGTTCCTGAAAAGATATGTGAATTATTTTGAGGAGGAAGCATCTACACTGTTAAGGCTATATCAGGGAGGATGCTAG

Protein sequence

>MS.gene066341.t1

IQTIIRRIPPIIVSNPIETILPKFQFLASKGASPQDIVVTVTKSPRFLCSSLDKQIIPAFDLVRSFCPSDQRAITIINSYPTSIARSRMKPNVHFLLDFGVNPSSIYYLLSSRPSIFCISDSDFRKAVEEIKGLGFHPSNGYFCVALRAKMAISKSHWDAKVDAFKSWGCSEDDILTAFKRKPSFMLRSRDKLNAVMRFWIKQHGWDPFVLLKAPDIFGFSIEKRLIPRASVVQYLLSKGLMKKSASLYTPFSLTDELFLKRYVNYFEEEASTLLRLYQGGC