AlfalfaGEDB | MS.gene066485

M. sativa cultivar XinJiangDaYe / MS.gene066485

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066485.t1	XP_003629092.1	84.1	170	27	0	1	170	1	170	5.40E-75	290.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066485.t1	Q8S9H7	52.1	48	23	0	103	150	132	179	9.8e-09	62.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066485.t1	G7L8N4	84.1	170	27	0	1	170	1	170	3.9e-75	290.8

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene066485.t1	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066485.t1	MTR_8g073050	90.625	224	20	1	1	224	1	223	3.19e-128	361
MS.gene066485.t1	MTR_6g043480	59.483	232	83	6	1	224	1	229	2.00e-72	220
MS.gene066485.t1	MTR_6g043490	60.515	233	79	6	1	224	1	229	9.89e-71	216
MS.gene066485.t1	MTR_8g072990	54.183	251	80	8	1	225	1	242	2.09e-65	202
MS.gene066485.t1	MTR_5g027570	40.580	69	41	0	98	166	87	155	6.77e-14	68.9
MS.gene066485.t1	MTR_8g063870	31.387	137	77	3	87	212	65	195	1.87e-12	65.5
MS.gene066485.t1	MTR_6g092540	32.787	122	64	2	103	224	97	200	3.60e-12	64.7
MS.gene066485.t1	MTR_1g048660	41.333	75	40	1	104	174	13	87	4.73e-12	60.5
MS.gene066485.t1	MTR_4g111975	47.170	53	28	0	100	152	135	187	6.86e-12	63.9
MS.gene066485.t1	MTR_8g101650	49.020	51	26	0	103	153	105	155	1.41e-11	62.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066485.t1	AT3G16350	49.153	59	29	1	93	150	126	184	2.90e-12	65.5
MS.gene066485.t1	AT5G05790	47.273	55	29	0	95	149	123	177	4.10e-12	64.3
MS.gene066485.t1	AT5G05790	47.273	55	29	0	95	149	123	177	4.10e-12	64.3
MS.gene066485.t1	AT5G58900	52.083	48	23	0	103	150	141	188	5.47e-12	64.3
MS.gene066485.t1	AT1G49010	47.170	53	28	0	100	152	131	183	6.48e-12	64.3
MS.gene066485.t1	AT2G38090	52.083	48	23	0	103	150	141	188	9.40e-12	63.5
MS.gene066485.t1	AT5G08520	45.283	53	29	0	100	152	115	167	1.96e-11	62.8
MS.gene066485.t1	AT5G61620	36.538	104	47	2	104	207	109	193	2.43e-11	62.4
MS.gene066485.t1	AT1G19000	49.091	55	27	1	96	149	94	148	2.68e-11	62.0
MS.gene066485.t1	AT1G19000	49.091	55	27	1	96	149	94	148	2.68e-11	62.0

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Find 73 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCATGCACAAATGTGGC+TGG	0.559050	8.2:-31247447	None:intergenic
AAAGATACAGGTGTTGATGG+AGG	0.610611	8.2:-31246489	None:intergenic
AAGACATCCCAATTCTCAAA+TGG	0.506263	8.2:+31246717	MS.gene066485:CDS
AAGATTAGGGTTGGTAGAAA+CGG	0.455787	8.2:-31246544	None:intergenic
AATTGGGATGTCTTTGGTTC+AGG	0.316369	8.2:-31246708	None:intergenic
ACAAGGTAGTTTGGTGTTGG+TGG	0.546899	8.2:-31246584	None:intergenic
ACTCCGCCTAGTGCTTCTGC+TGG	0.556890	8.2:+31247603	MS.gene066485:CDS
ACTCTGCTTCCAAGAGAAAC+AGG	0.420387	8.2:+31247502	MS.gene066485:CDS
AGAAGCACTAGGCGGAGTCA+TGG	0.531695	8.2:-31247598	None:intergenic
AGATTAGGGTTGGTAGAAAC+GGG	0.501786	8.2:-31246543	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAGAAAGTTGAAATAACTG+CGG	-	chr8.2:31246986-31247005	None:intergenic	30.0%
GGTACATTAGATAAAGATAC+AGG	-	chr8.2:31246504-31246523	None:intergenic	30.0%
GTAAGATCAATGATCTCAAT+AGG	-	chr8.2:31246453-31246472	None:intergenic	30.0%
TAGAAACGGGAACAAAATTA+AGG	-	chr8.2:31246533-31246552	None:intergenic	30.0%
AAATAACTGCGGATTTAGTG+AGG	-	chr8.2:31246975-31246994	None:intergenic	35.0%
AAGACATCCCAATTCTCAAA+TGG	+	chr8.2:31246717-31246736	MS.gene066485:CDS	35.0%
AAGGATCTCACTTTACTACT+GGG	+	chr8.2:31246822-31246841	MS.gene066485:intron	35.0%
CCACAACAAGTTAAAAGCTA+TGG	+	chr8.2:31247402-31247421	MS.gene066485:CDS	35.0%
GGAACAAAATTAAGGATCGT+TGG	-	chr8.2:31246525-31246544	None:intergenic	35.0%
GTTTAAGAACCGTTGCAATA+TGG	-	chr8.2:31246683-31246702	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	31246447	31247668	31246447	ID=MS.gene066485
chr8.2	mRNA	31246447	31247668	31246447	ID=MS.gene066485.t1;Parent=MS.gene066485
chr8.2	exon	31246447	31246778	31246447	ID=MS.gene066485.t1.exon1;Parent=MS.gene066485.t1
chr8.2	CDS	31246447	31246778	31246447	ID=cds.MS.gene066485.t1;Parent=MS.gene066485.t1
chr8.2	exon	31247323	31247668	31247323	ID=MS.gene066485.t1.exon2;Parent=MS.gene066485.t1
chr8.2	CDS	31247323	31247668	31247323	ID=cds.MS.gene066485.t1;Parent=MS.gene066485.t1

Gene Sequence

>MS.gene066485

ATGCCTATTGAGATCATTGATCTTACAAATTTCCCTGACTCACCTCCATCAACACCTGTATCTTTATCTAATGTACCAACGATCCTTAATTTTGTTCCCGTTTCTACCAACCCTAATCTTCACCACCAAATCCATCACCACCAACACCAAACTACCTTGTTTGTACCACCACCTCCAGGATTTGAGACCCCACAAATCGCTACCGGTTGGCCCCAACCTGGCATGTATCACAGCCATATTGCAACGGTTCTTAAACCGTTACCTGAACCAAAGACATCCCAATTCTCAAATGGAGCGAGGCTCAGGGTTCCTTGGAGTCAATTAGAGCATGAGTAAGTACATCTTTCAAACTTAAATCTAATTAAGTTTGTATGTAAGGATCTCACTTTACTACTGGGAAAAAGTTGAAATTGCGATAAAAATTTAGAGGCACCATTTTTAGTGATTAATTTTTGTTATAATTTTTTGCTTCTTATTTCCGCAGTTATTATTTTTTTGACAATGTTTTTCTAACTGTCGTTTTTACCTCACTAAATCCGCAGTTATTTCAACTTTCTCTTATAGTGCATTTCTTACTTTCGTTTCACTATTATAAAACGTTGAAATAGCCACAAAAATTTGGAGAAAGAAAAATACGAAATTTTTATGTCTCTAATTCTATATTAAATTTTTAGACGCCAAATTTAATGACTAATTTTATTTTTTCATCTCTAATATCTGTCGTGATTTCAATTTTTGTATCGATTTTCTTAAAATCACTTTTACACCAATTACTCTTTCATAGATTATAAAGTAATGTGCAAGCATTGATTTTATTTTTCTCCCGTTAAATTTTACTCTTGATTTTTATTAGAATTAATTTTTTATTTATTTTAGCTTATTTCTGATGGGGCTGATTGAGTATGGAAAAGGGAAATGGAGCAAAATTGCTAAGAACTATGTATCTAGCAAGACACCACAACAAGTTAAAAGCTATGGTTTAAGCTTCTTCATGTACCTACCAGCCACATTTGTGCATGGTTTTAGGAAAAGGAAACTAATTGCAAATCCCAACAACTCTGCTTCCAAGAGAAACAGGAACTCATCATCTGCTCCATATTATGGAGAAGCTAGCAGCAGCAAGAATACCAACAACTATGAAGCAAGCACTTCCATGACTCCGCCTAGTGCTTCTGCTGGTGATGGAGAAGTTGATTTGGAGCTTCGTTTGGGTTTATATTAG

Protein sequence

>MS.gene066485.t1

MPIEIIDLTNFPDSPPSTPVSLSNVPTILNFVPVSTNPNLHHQIHHHQHQTTLFVPPPPGFETPQIATGWPQPGMYHSHIATVLKPLPEPKTSQFSNGARLRVPWSQLEHDLFLMGLIEYGKGKWSKIAKNYVSSKTPQQVKSYGLSFFMYLPATFVHGFRKRKLIANPNNSASKRNRNSSSAPYYGEASSSKNTNNYEASTSMTPPSASAGDGEVDLELRLGLY