AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066525

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066525.t1 MTR_3g104750 96.591 88 3 0 11 98 77 164 1.26e-59 180
MS.gene066525.t1 MTR_1g015140 78.313 83 17 1 14 96 79 160 2.98e-43 138
MS.gene066525.t1 MTR_8g092010 69.136 81 21 1 14 94 88 164 1.97e-35 119
MS.gene066525.t1 MTR_1g086790 63.095 84 31 0 13 96 133 216 5.09e-33 116
MS.gene066525.t1 MTR_7g109800 59.524 84 34 0 13 96 120 203 2.83e-32 114
MS.gene066525.t1 MTR_2g033820 69.333 75 23 0 22 96 187 261 1.27e-31 114
MS.gene066525.t1 MTR_3g095040 66.667 72 24 0 25 96 105 176 6.55e-31 108
MS.gene066525.t1 MTR_4g094588 67.606 71 23 0 25 95 165 235 1.57e-30 110
MS.gene066525.t1 MTR_5g043880 66.250 80 26 1 15 94 102 180 3.01e-30 108
MS.gene066525.t1 MTR_3g095080 60.811 74 29 0 21 94 151 224 1.22e-29 106
MS.gene066525.t1 MTR_3g095080 60.811 74 29 0 21 94 127 200 1.23e-29 105
MS.gene066525.t1 MTR_8g096730 57.143 77 33 0 19 95 51 127 1.74e-29 103
MS.gene066525.t1 MTR_4g122530 62.667 75 28 0 22 96 192 266 3.27e-29 108
MS.gene066525.t1 MTR_7g028415 63.768 69 25 0 27 95 82 150 2.25e-28 100
MS.gene066525.t1 MTR_7g028710 63.768 69 25 0 27 95 82 150 2.25e-28 100
MS.gene066525.t1 MTR_5g094430 59.211 76 31 0 19 94 211 286 7.36e-28 104
MS.gene066525.t1 MTR_5g094430 51.724 58 27 1 38 95 66 122 5.42e-12 60.5
MS.gene066525.t1 MTR_5g094430 59.211 76 31 0 19 94 345 420 8.18e-28 105
MS.gene066525.t1 MTR_5g094430 51.724 58 27 1 38 95 200 256 4.91e-12 60.8
MS.gene066525.t1 MTR_5g094430 59.211 76 31 0 19 94 233 308 9.27e-28 104
MS.gene066525.t1 MTR_5g094430 51.724 58 27 1 38 95 88 144 4.82e-12 60.5
MS.gene066525.t1 MTR_5g091390 65.714 70 24 0 25 94 144 213 9.86e-28 102
MS.gene066525.t1 MTR_3g056100 60.526 76 30 0 19 94 239 314 1.11e-27 104
MS.gene066525.t1 MTR_3g056100 51.724 58 27 1 38 95 99 155 2.64e-12 61.2
MS.gene066525.t1 MTR_3g056100 60.526 76 30 0 19 94 383 458 1.12e-27 105
MS.gene066525.t1 MTR_3g056100 51.724 58 27 1 38 95 243 299 2.29e-12 61.6
MS.gene066525.t1 MTR_3g009470 58.228 79 33 0 16 94 473 551 1.17e-27 105
MS.gene066525.t1 MTR_3g009470 50.000 58 28 1 38 95 280 336 1.97e-13 64.7
MS.gene066525.t1 MTR_3g056100 60.526 76 30 0 19 94 252 327 1.27e-27 104
MS.gene066525.t1 MTR_3g056100 51.724 58 27 1 38 95 99 155 2.58e-12 61.6
MS.gene066525.t1 MTR_3g056100 60.526 76 30 0 19 94 396 471 1.28e-27 105
MS.gene066525.t1 MTR_3g056100 51.724 58 27 1 38 95 243 299 2.29e-12 61.6
MS.gene066525.t1 MTR_8g007270 55.844 77 34 0 18 94 134 210 3.84e-27 99.4
MS.gene066525.t1 MTR_7g011820 55.844 77 34 0 18 94 390 466 4.20e-27 103
MS.gene066525.t1 MTR_7g011820 50.877 57 27 1 38 94 228 283 1.27e-12 62.4
MS.gene066525.t1 MTR_8g092140 58.667 75 30 1 21 94 142 216 4.81e-27 99.4
MS.gene066525.t1 MTR_5g042920 55.263 76 34 0 19 94 301 376 3.50e-26 100
MS.gene066525.t1 MTR_5g042920 50.877 57 27 1 38 94 151 206 4.55e-12 60.8
MS.gene066525.t1 MTR_3g031220 53.571 84 39 0 11 94 338 421 4.72e-26 100
MS.gene066525.t1 MTR_3g031220 47.222 72 33 2 28 95 184 254 2.73e-16 72.8
MS.gene066525.t1 MTR_4g013260 55.263 76 34 0 19 94 317 392 6.38e-26 99.8
MS.gene066525.t1 MTR_4g013260 51.724 58 28 0 38 95 184 241 1.28e-16 73.6
MS.gene066525.t1 MTR_5g074400 51.190 84 41 0 11 94 393 476 6.41e-26 100
MS.gene066525.t1 MTR_5g074400 53.448 58 26 1 38 95 243 299 4.39e-16 72.0
MS.gene066525.t1 MTR_7g079010 57.692 78 33 0 18 95 121 198 9.30e-26 95.5
MS.gene066525.t1 MTR_7g080020 52.632 76 36 0 19 94 474 549 1.12e-25 99.4
MS.gene066525.t1 MTR_7g080020 44.048 84 39 3 15 98 264 339 1.79e-14 67.8
MS.gene066525.t1 MTR_7g080000 50.649 77 38 0 18 94 412 488 1.90e-25 99.0
MS.gene066525.t1 MTR_7g080000 46.250 80 35 3 15 94 218 289 2.43e-14 67.4
MS.gene066525.t1 MTR_7g071120 55.263 76 34 0 19 94 305 380 2.44e-25 98.2
MS.gene066525.t1 MTR_7g071120 55.172 58 25 1 38 95 166 222 3.65e-16 72.4
MS.gene066525.t1 MTR_4g132430 51.316 76 36 1 21 95 110 185 9.21e-25 95.5
MS.gene066525.t1 MTR_4g132430 51.316 76 36 1 21 95 244 319 3.39e-24 95.1
MS.gene066525.t1 MTR_4g082580 55.714 70 31 0 25 94 265 334 3.82e-24 94.4
MS.gene066525.t1 MTR_4g082580 49.123 57 29 0 38 94 145 201 1.68e-12 62.0
MS.gene066525.t1 MTR_5g097490 58.333 72 30 0 23 94 237 308 1.18e-23 92.8
MS.gene066525.t1 MTR_2g023930 48.684 76 38 1 21 95 288 363 4.79e-23 92.0
MS.gene066525.t1 MTR_7g100510 53.623 69 32 0 26 94 349 417 8.29e-23 90.9
MS.gene066525.t1 MTR_7g100510 51.786 56 26 1 39 94 190 244 1.74e-12 62.0
MS.gene066525.t1 MTR_1g078260 54.167 72 32 1 24 94 233 304 2.85e-22 89.7
MS.gene066525.t1 MTR_7g110720 50.000 72 35 1 25 95 176 247 3.12e-22 89.4
MS.gene066525.t1 MTR_2g105060 52.703 74 32 2 25 95 79 152 3.39e-22 86.7
MS.gene066525.t1 MTR_7g110720 50.000 72 35 1 25 95 203 274 3.78e-22 89.0
MS.gene066525.t1 MTR_4g094412 52.000 75 35 1 22 95 218 292 3.81e-22 89.4
MS.gene066525.t1 MTR_7g062220 60.000 60 24 0 38 97 124 183 4.53e-22 87.8
MS.gene066525.t1 MTR_4g094412 52.000 75 35 1 22 95 227 301 4.89e-22 89.0
MS.gene066525.t1 MTR_2g105060 52.703 74 32 2 25 95 154 227 5.68e-22 87.4
MS.gene066525.t1 MTR_3g053330 43.529 85 47 1 11 94 224 308 8.86e-22 88.2
MS.gene066525.t1 MTR_2g088000 48.000 75 38 1 21 94 332 406 2.08e-21 87.4
MS.gene066525.t1 MTR_1g077780 46.575 73 39 0 22 94 46 118 3.58e-21 82.0
MS.gene066525.t1 MTR_6g086555 49.296 71 35 1 25 94 193 263 1.04e-20 85.1
MS.gene066525.t1 MTR_7g009730 51.389 72 34 1 25 95 206 277 1.96e-20 84.7
MS.gene066525.t1 MTR_4g130900 59.677 62 25 0 33 94 354 415 3.24e-20 84.0
MS.gene066525.t1 MTR_4g107970 49.296 71 35 1 25 94 165 235 2.43e-19 81.3
MS.gene066525.t1 MTR_5g016610 49.296 71 35 1 25 94 186 256 2.74e-19 79.7
MS.gene066525.t1 MTR_4g107970 49.296 71 35 1 25 94 242 312 6.01e-19 80.5
MS.gene066525.t1 MTR_1g013760 44.737 76 39 2 22 94 122 197 1.30e-18 78.2
MS.gene066525.t1 MTR_4g007060 52.459 61 28 1 36 95 126 186 1.36e-18 78.2
MS.gene066525.t1 MTR_1g009613 48.718 78 36 2 18 94 245 319 1.89e-18 78.2
MS.gene066525.t1 MTR_4g007060 52.459 61 28 1 36 95 160 220 2.05e-18 77.8
MS.gene066525.t1 MTR_2g045360 47.297 74 38 1 22 94 195 268 2.82e-18 78.2
MS.gene066525.t1 MTR_3g106060 52.632 57 26 1 39 94 139 195 2.96e-18 77.4
MS.gene066525.t1 MTR_8g028565 42.529 87 47 3 11 96 130 214 5.10e-18 77.0
MS.gene066525.t1 MTR_4g049760 48.611 72 36 1 25 95 277 348 5.84e-18 77.4
MS.gene066525.t1 MTR_4g049790 48.611 72 36 1 25 95 299 370 1.21e-17 76.6
MS.gene066525.t1 MTR_4g094908 46.154 78 38 2 18 94 249 323 1.45e-17 75.9
MS.gene066525.t1 MTR_4g127040 47.436 78 37 2 18 94 228 302 2.29e-17 75.1
MS.gene066525.t1 MTR_1g070455 45.946 74 39 1 22 94 277 350 6.56e-17 74.3
MS.gene066525.t1 MTR_7g109600 56.897 58 24 1 39 95 54 111 9.56e-17 72.8
MS.gene066525.t1 MTR_3g031220 47.222 72 33 2 28 95 184 254 2.11e-16 72.8
MS.gene066525.t1 MTR_3g031220 50.000 48 24 0 11 58 338 385 5.73e-11 57.4
MS.gene066525.t1 MTR_5g097500 46.377 69 37 0 26 94 218 286 2.87e-16 72.0
MS.gene066525.t1 MTR_0117s0040 47.692 65 33 1 34 97 70 134 8.48e-16 70.1
MS.gene066525.t1 MTR_3g108960 40.741 81 47 1 15 94 291 371 8.88e-16 71.2
MS.gene066525.t1 MTR_2g032030 51.724 58 27 1 39 95 268 325 2.63e-15 70.1
MS.gene066525.t1 MTR_2g032030 51.724 58 27 1 39 95 268 325 3.04e-15 69.7
MS.gene066525.t1 MTR_7g079040 42.697 89 47 3 10 96 127 213 3.63e-15 69.3
MS.gene066525.t1 MTR_1g013790 45.000 60 32 1 36 94 99 158 3.98e-15 68.6
MS.gene066525.t1 MTR_8g098945 51.667 60 28 1 37 95 128 187 5.02e-15 68.2
MS.gene066525.t1 MTR_8g087000 52.632 57 26 1 39 94 78 134 1.79e-14 66.6
MS.gene066525.t1 MTR_8g087000 52.632 57 26 1 39 94 77 133 1.83e-14 66.6
MS.gene066525.t1 MTR_2g083870 46.269 67 35 1 29 94 71 137 2.52e-14 66.2
MS.gene066525.t1 MTR_8g092010 56.863 51 18 1 14 64 88 134 2.72e-14 63.9
MS.gene066525.t1 MTR_5g074200 45.588 68 34 2 29 94 209 275 5.04e-14 65.9
MS.gene066525.t1 MTR_3g090860 48.333 60 28 1 37 93 132 191 7.73e-14 65.5
MS.gene066525.t1 MTR_2g016770 49.123 57 28 1 39 94 36 92 1.38e-13 63.9
MS.gene066525.t1 MTR_2g016770 49.123 57 28 1 39 94 28 84 1.61e-13 63.5
MS.gene066525.t1 MTR_1g099600 50.877 57 27 1 39 94 186 242 2.72e-13 63.9
MS.gene066525.t1 MTR_3g009470 50.000 58 28 1 38 95 280 336 4.03e-13 63.9
MS.gene066525.t1 MTR_3g009470 60.465 43 17 0 16 58 473 515 1.19e-12 62.4
MS.gene066525.t1 MTR_4g047580 40.278 72 42 1 25 95 268 339 6.72e-13 63.2
MS.gene066525.t1 MTR_2g024380 44.928 69 35 2 33 98 246 314 1.02e-12 62.8
MS.gene066525.t1 MTR_2g024350 44.444 63 34 1 33 94 3 65 3.57e-12 60.1
MS.gene066525.t1 MTR_5g044695 46.667 45 24 0 21 65 60 104 5.11e-12 57.4
MS.gene066525.t1 MTR_5g029850 61.702 47 17 1 9 55 23 68 1.15e-11 55.8
MS.gene066525.t1 MTR_7g073430 47.458 59 28 1 38 93 122 180 1.85e-11 58.5
MS.gene066525.t1 MTR_8g099350 42.373 59 31 1 38 93 132 190 2.77e-11 58.5
MS.gene066525.t1 MTR_8g067635 45.161 62 31 3 35 94 346 406 3.40e-11 58.2
MS.gene066525.t1 MTR_2g075680 43.939 66 34 1 31 93 141 206 4.07e-11 57.8
MS.gene066525.t1 MTR_2g075750 36.842 76 44 2 21 93 12 86 4.46e-11 56.6
MS.gene066525.t1 MTR_8g067650 45.902 61 30 3 36 94 337 396 4.73e-11 57.8
MS.gene066525.t1 MTR_5g067680 44.828 58 29 1 38 92 202 259 4.78e-11 57.8
MS.gene066525.t1 MTR_5g018380 44.262 61 32 1 35 95 120 178 5.52e-11 56.2
MS.gene066525.t1 MTR_6g038890 46.667 45 24 0 21 65 171 215 5.55e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066525.t1 AT5G26170 81.081 74 14 0 25 98 100 173 5.94e-42 135
MS.gene066525.t1 AT5G64810 67.568 74 24 0 24 97 96 169 1.10e-34 117
MS.gene066525.t1 AT2G47260 63.095 84 31 0 13 96 149 232 1.23e-32 115
MS.gene066525.t1 AT4G18170 62.353 85 32 0 11 95 145 229 2.35e-32 115
MS.gene066525.t1 AT5G49520 68.919 74 23 0 22 95 205 278 3.26e-32 115
MS.gene066525.t1 AT1G29860 67.568 74 24 0 22 95 120 193 1.58e-31 112
MS.gene066525.t1 AT4G39410 62.195 82 30 1 18 98 203 284 5.21e-31 111
MS.gene066525.t1 AT1G69310 65.000 80 28 0 15 94 124 203 6.69e-31 110
MS.gene066525.t1 AT1G69310 65.000 80 28 0 15 94 124 203 6.69e-31 110
MS.gene066525.t1 AT1G69310 65.000 80 28 0 15 94 124 203 6.69e-31 110
MS.gene066525.t1 AT3G62340 64.286 84 28 1 12 95 94 175 1.27e-30 109
MS.gene066525.t1 AT1G64000 54.217 83 37 1 14 95 89 171 9.15e-29 102
MS.gene066525.t1 AT5G46350 64.789 71 25 0 25 95 170 240 9.19e-29 105
MS.gene066525.t1 AT5G13080 59.722 72 29 0 24 95 53 124 1.71e-28 100
MS.gene066525.t1 AT2G46130 50.575 87 43 0 9 95 1 87 3.85e-28 98.6
MS.gene066525.t1 AT3G01970 60.000 70 28 0 25 94 52 121 4.51e-28 99.8
MS.gene066525.t1 AT4G26640 60.526 76 30 0 19 94 186 261 6.39e-28 104
MS.gene066525.t1 AT4G26640 50.000 60 29 1 36 95 33 91 1.51e-12 62.0
MS.gene066525.t1 AT4G26640 60.526 76 30 0 19 94 186 261 6.39e-28 104
MS.gene066525.t1 AT4G26640 50.000 60 29 1 36 95 33 91 1.51e-12 62.0
MS.gene066525.t1 AT4G26640 60.526 76 30 0 19 94 186 261 6.39e-28 104
MS.gene066525.t1 AT4G26640 50.000 60 29 1 36 95 33 91 1.51e-12 62.0
MS.gene066525.t1 AT4G26640 60.526 76 30 0 19 94 186 261 6.39e-28 104
MS.gene066525.t1 AT4G26640 50.000 60 29 1 36 95 33 91 1.51e-12 62.0
MS.gene066525.t1 AT4G26640 60.526 76 30 0 19 94 322 397 7.59e-28 105
MS.gene066525.t1 AT4G26640 50.000 60 29 1 36 95 169 227 1.08e-12 62.8
MS.gene066525.t1 AT4G26640 60.526 76 30 0 19 94 290 365 7.85e-28 105
MS.gene066525.t1 AT4G26640 50.000 60 29 1 36 95 137 195 1.10e-12 62.4
MS.gene066525.t1 AT4G26640 60.526 76 30 0 19 94 362 437 8.53e-28 105
MS.gene066525.t1 AT4G26640 50.000 60 29 1 36 95 209 267 9.83e-13 62.8
MS.gene066525.t1 AT2G44745 57.143 77 33 0 18 94 125 201 1.09e-27 100
MS.gene066525.t1 AT5G07100 56.757 74 32 0 21 94 124 197 3.08e-27 99.4
MS.gene066525.t1 AT5G07100 46.552 58 31 0 38 95 24 81 3.79e-14 65.5
MS.gene066525.t1 AT5G07100 56.757 74 32 0 21 94 124 197 3.08e-27 99.4
MS.gene066525.t1 AT5G07100 46.552 58 31 0 38 95 24 81 3.79e-14 65.5
MS.gene066525.t1 AT5G07100 56.757 74 32 0 21 94 124 197 3.08e-27 99.4
MS.gene066525.t1 AT5G07100 46.552 58 31 0 38 95 24 81 3.79e-14 65.5
MS.gene066525.t1 AT5G07100 56.757 74 32 0 21 94 124 197 3.08e-27 99.4
MS.gene066525.t1 AT5G07100 46.552 58 31 0 38 95 24 81 3.79e-14 65.5
MS.gene066525.t1 AT5G56270 56.962 79 34 0 16 94 465 543 5.39e-27 103
MS.gene066525.t1 AT5G56270 50.877 57 27 1 38 94 273 328 1.61e-12 62.0
MS.gene066525.t1 AT5G07100 54.545 77 35 0 18 94 214 290 7.15e-27 100
MS.gene066525.t1 AT5G07100 46.552 58 31 0 38 95 117 174 8.09e-14 65.5
MS.gene066525.t1 AT4G26440 54.430 79 36 0 16 94 350 428 7.15e-27 102
MS.gene066525.t1 AT4G26440 47.368 57 29 1 38 94 178 233 6.23e-13 63.2
MS.gene066525.t1 AT5G41570 53.165 79 37 0 17 95 77 155 7.62e-27 97.4
MS.gene066525.t1 AT2G21900 46.602 103 47 2 2 96 65 167 3.93e-26 96.3
MS.gene066525.t1 AT1G69310 61.250 80 27 1 15 94 124 199 7.80e-26 97.1
MS.gene066525.t1 AT1G13960 52.632 76 36 0 19 94 390 465 1.33e-25 99.0
MS.gene066525.t1 AT1G13960 54.386 57 25 1 38 94 229 284 1.34e-14 68.2
MS.gene066525.t1 AT2G38470 55.263 76 34 0 19 94 343 418 1.73e-25 99.0
MS.gene066525.t1 AT2G38470 50.000 58 28 1 38 95 184 240 3.93e-14 66.6
MS.gene066525.t1 AT1G13960 52.632 76 36 0 19 94 237 312 5.83e-25 96.3
MS.gene066525.t1 AT1G13960 54.386 57 25 1 38 94 76 131 6.46e-14 65.9
MS.gene066525.t1 AT3G01080 52.326 86 39 1 9 94 317 400 7.04e-25 97.1
MS.gene066525.t1 AT3G01080 49.123 57 28 1 38 94 205 260 9.86e-11 57.0
MS.gene066525.t1 AT3G01080 52.326 86 39 1 9 94 279 362 7.83e-25 96.7
MS.gene066525.t1 AT2G03340 51.316 76 37 0 19 94 396 471 4.16e-24 95.1
MS.gene066525.t1 AT2G03340 52.632 57 26 1 38 94 250 305 2.16e-13 64.7
MS.gene066525.t1 AT2G30250 52.632 76 36 0 19 94 309 384 1.39e-23 92.8
MS.gene066525.t1 AT2G30250 35.000 100 49 3 11 95 124 222 4.81e-11 57.8
MS.gene066525.t1 AT2G46130 47.126 87 42 1 9 95 1 83 5.28e-23 85.5
MS.gene066525.t1 AT4G04450 44.706 85 46 1 12 95 172 256 8.10e-23 90.9
MS.gene066525.t1 AT4G22070 44.706 85 46 1 12 95 271 355 1.26e-22 90.9
MS.gene066525.t1 AT4G22070 44.706 85 46 1 12 95 271 355 1.26e-22 90.9
MS.gene066525.t1 AT4G22070 44.706 85 46 1 12 95 271 355 1.26e-22 90.9
MS.gene066525.t1 AT4G22070 44.706 85 46 1 12 95 271 355 1.26e-22 90.9
MS.gene066525.t1 AT1G55600 45.349 86 46 1 12 96 280 365 1.29e-22 90.5
MS.gene066525.t1 AT2G04880 51.948 77 37 0 18 94 287 363 3.13e-22 89.7
MS.gene066525.t1 AT2G04880 45.000 60 32 1 36 95 109 167 1.35e-11 59.3
MS.gene066525.t1 AT2G04880 51.948 77 37 0 18 94 263 339 3.20e-22 89.4
MS.gene066525.t1 AT2G04880 45.000 60 32 1 36 95 109 167 1.35e-11 59.3
MS.gene066525.t1 AT4G04450 44.706 85 46 1 12 95 266 350 3.74e-22 89.4
MS.gene066525.t1 AT1G62300 50.000 76 37 1 21 95 295 370 5.78e-22 89.0
MS.gene066525.t1 AT5G15130 46.250 80 42 1 16 94 163 242 7.57e-22 88.6
MS.gene066525.t1 AT5G15130 46.250 80 42 1 16 94 205 284 8.26e-22 88.6
MS.gene066525.t1 AT1G68150 50.667 75 36 1 22 95 219 293 6.10e-21 85.5
MS.gene066525.t1 AT2G25000 52.459 61 28 1 39 98 104 164 6.59e-21 83.2
MS.gene066525.t1 AT2G25000 52.459 61 28 1 39 98 104 164 6.59e-21 83.2
MS.gene066525.t1 AT2G25000 52.459 61 28 1 39 98 126 186 1.61e-20 82.8
MS.gene066525.t1 AT2G25000 52.459 61 28 1 39 98 147 207 1.95e-20 82.8
MS.gene066525.t1 AT4G31800 56.140 57 24 1 39 94 112 168 2.15e-20 82.4
MS.gene066525.t1 AT4G01720 43.373 83 46 1 14 95 215 297 4.51e-20 83.6
MS.gene066525.t1 AT4G31800 42.857 84 47 1 12 94 150 233 6.62e-20 82.0
MS.gene066525.t1 AT5G43290 55.932 59 26 0 36 94 112 170 1.18e-19 80.9
MS.gene066525.t1 AT4G30935 47.619 84 39 1 11 94 309 387 1.22e-19 82.4
MS.gene066525.t1 AT2G37260 56.452 62 27 0 33 94 264 325 1.53e-19 81.3
MS.gene066525.t1 AT2G37260 46.667 75 35 2 24 94 67 140 1.25e-12 62.4
MS.gene066525.t1 AT2G37260 56.452 62 27 0 33 94 262 323 1.70e-19 81.3
MS.gene066525.t1 AT2G37260 46.667 75 35 2 24 94 65 138 1.40e-12 62.0
MS.gene066525.t1 AT2G37260 56.452 62 27 0 33 94 344 405 2.21e-19 81.3
MS.gene066525.t1 AT2G37260 46.667 75 35 2 24 94 147 220 1.24e-12 62.4
MS.gene066525.t1 AT1G18860 44.737 76 41 1 21 95 222 297 4.13e-19 80.9
MS.gene066525.t1 AT1G80840 43.243 74 39 2 25 95 131 204 2.23e-18 77.8
MS.gene066525.t1 AT4G31550 54.098 61 27 1 35 94 242 302 1.10e-17 76.3
MS.gene066525.t1 AT4G31550 54.098 61 27 1 35 94 243 303 1.11e-17 76.3
MS.gene066525.t1 AT4G24240 45.783 83 44 1 13 94 256 338 1.12e-17 76.3
MS.gene066525.t1 AT2G23320 45.783 83 44 1 13 94 215 297 1.66e-17 75.5
MS.gene066525.t1 AT3G04670 48.649 74 37 1 22 94 246 319 3.61e-17 74.7
MS.gene066525.t1 AT2G30590 44.156 77 42 1 22 97 297 373 5.36e-17 74.7
MS.gene066525.t1 AT5G28650 48.649 74 37 1 22 94 246 319 6.63e-17 73.9
MS.gene066525.t1 AT5G52830 58.621 58 23 1 39 95 166 223 7.30e-17 73.9
MS.gene066525.t1 AT2G24570 46.154 78 38 2 18 94 226 300 7.55e-17 73.9
MS.gene066525.t1 AT1G29280 53.448 58 26 1 39 95 75 132 7.74e-17 73.2
MS.gene066525.t1 AT4G01250 39.773 88 50 2 10 96 102 187 1.09e-16 73.2
MS.gene066525.t1 AT2G34830 46.479 71 36 2 27 95 212 282 4.08e-16 72.4
MS.gene066525.t1 AT2G34830 46.479 71 36 2 27 95 203 273 4.24e-16 72.0
MS.gene066525.t1 AT1G30650 46.479 71 36 2 27 95 205 275 5.88e-16 71.6
MS.gene066525.t1 AT3G58710 50.000 68 33 1 29 95 61 128 7.09e-16 70.5
MS.gene066525.t1 AT3G58710 50.000 68 33 1 29 95 62 129 7.23e-16 70.5
MS.gene066525.t1 AT1G69810 43.210 81 44 2 17 95 124 204 1.43e-15 70.5
MS.gene066525.t1 AT1G69810 43.210 81 44 2 17 95 182 262 1.50e-15 70.5
MS.gene066525.t1 AT5G45050 47.297 74 32 2 29 95 1026 1099 1.57e-15 70.9
MS.gene066525.t1 AT5G45050 47.297 74 32 2 29 95 1165 1238 2.16e-15 70.5
MS.gene066525.t1 AT5G45050 47.297 74 32 2 29 95 1137 1210 2.40e-15 70.1
MS.gene066525.t1 AT4G23550 47.619 63 32 1 33 94 135 197 1.45e-14 67.4
MS.gene066525.t1 AT3G01080 49.123 57 28 1 38 94 205 260 3.75e-12 60.8
MS.gene066525.t1 AT1G66560 50.000 58 26 1 38 92 103 160 3.82e-12 60.5
MS.gene066525.t1 AT1G66600 48.438 64 30 1 37 97 102 165 4.79e-12 60.1
MS.gene066525.t1 AT3G56400 42.188 64 34 1 33 93 115 178 1.10e-11 59.3
MS.gene066525.t1 AT2G46400 43.056 72 38 1 25 93 91 162 1.55e-11 58.9
MS.gene066525.t1 AT5G01900 35.802 81 49 1 15 92 87 167 2.97e-11 58.2
MS.gene066525.t1 AT2G40740 47.458 59 28 1 38 93 182 240 3.69e-11 58.2
MS.gene066525.t1 AT4G11070 45.763 59 29 1 38 93 141 199 3.91e-11 58.2
MS.gene066525.t1 AT5G45260 34.940 83 49 2 18 95 1186 1268 6.94e-11 57.4
MS.gene066525.t1 AT1G66550 40.000 75 42 1 26 97 91 165 7.84e-11 56.6
MS.gene066525.t1 AT1G80590 46.552 58 28 1 39 93 86 143 7.98e-11 56.6
MS.gene066525.t1 AT1G66550 40.000 75 42 1 26 97 96 170 8.36e-11 56.6

Find 25 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGTCAGAGGTTGAAATTC+TGG 0.266650 3.3:+86168725 MS.gene066525:CDS
TTGGTTTGTGTCATATTTAT+AGG 0.324455 3.3:+86168642 MS.gene066525:CDS
ATAGGAGGTGTCAGCAATAA+AGG 0.363243 3.3:+86168660 MS.gene066525:CDS
TTGTTTACAGGAACTACTAT+AGG 0.382242 3.3:+86168948 MS.gene066525:intron
CAGAATTTCAACCTCTGACT+TGG 0.388820 3.3:-86168724 None:intergenic
TGTAGATGCTACCTTGGATT+GGG 0.399112 3.3:-86168798 None:intergenic
GAGGTTGAAATTCTGGATGA+TGG 0.407475 3.3:+86168732 MS.gene066525:CDS
AAATTCTGGATGATGGGTTC+AGG 0.414441 3.3:+86168739 MS.gene066525:CDS
TAGGTTGTTATTACATAACT+TGG 0.446464 3.3:-86169025 None:intergenic
AACTTGATCTAGTGAAAACT+TGG 0.453876 3.3:-86169067 None:intergenic
ATGTAGATGCTACCTTGGAT+TGG 0.508242 3.3:-86168799 None:intergenic
TGGATGATGGGTTCAGGTGG+AGG 0.514242 3.3:+86168745 MS.gene066525:CDS
TACTATAGGTGTTCAGTGGA+TGG 0.515134 3.3:+86168962 MS.gene066525:CDS
GGGTTCAGGTGGAGGAAGTA+CGG 0.526912 3.3:+86168753 MS.gene066525:CDS
AGGTTGAAATTCTGGATGAT+GGG 0.535485 3.3:+86168733 MS.gene066525:CDS
CAGCAATAAAGGTGAGAAGA+AGG 0.542475 3.3:+86168671 MS.gene066525:CDS
GAGGAAGTACGGAAAGAAGA+TGG 0.552357 3.3:+86168764 MS.gene066525:CDS
GTTTGTGTCATATTTATAGG+AGG 0.565606 3.3:+86168645 MS.gene066525:CDS
AAAGAGAGTTGAGAGAGATG+TGG 0.568317 3.3:+86168997 MS.gene066525:CDS
TTCTGGATGATGGGTTCAGG+TGG 0.569211 3.3:+86168742 MS.gene066525:CDS
TGCATTCAAAACCAAGTCAG+AGG 0.595589 3.3:+86168713 MS.gene066525:CDS
ATGAGTATGTGTGCCTTCGT+AGG 0.623197 3.3:-86169044 None:intergenic
GAACTACTATAGGTGTTCAG+TGG 0.652914 3.3:+86168958 MS.gene066525:CDS
GTAGATGCTACCTTGGATTG+GGG 0.663888 3.3:-86168797 None:intergenic
TATGTAATAACAACCTACGA+AGG 0.679326 3.3:+86169031 MS.gene066525:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATACATAATATATAAGAGA+TGG - chr3.3:86168904-86168923 None:intergenic 15.0%
!! GAATTTCCAAATTTGTTTAC+AGG + chr3.3:86168936-86168955 MS.gene066525:intron 25.0%
!! TAGGTTGTTATTACATAACT+TGG - chr3.3:86169028-86169047 None:intergenic 25.0%
!!! TTGGTTTGTGTCATATTTAT+AGG + chr3.3:86168642-86168661 MS.gene066525:CDS 25.0%
GTAGTTCCTGTAAACAAATT+TGG - chr3.3:86168945-86168964 None:intergenic 30.0%
GTTTGTGTCATATTTATAGG+AGG + chr3.3:86168645-86168664 MS.gene066525:CDS 30.0%
TATGTAATAACAACCTACGA+AGG + chr3.3:86169031-86169050 MS.gene066525:CDS 30.0%
TTGTTTACAGGAACTACTAT+AGG + chr3.3:86168948-86168967 MS.gene066525:intron 30.0%
AGGTTGAAATTCTGGATGAT+GGG + chr3.3:86168733-86168752 MS.gene066525:CDS 35.0%
!! TCTCAACTCTCTTTTTCACT+TGG - chr3.3:86168991-86169010 None:intergenic 35.0%
AAAGAGAGTTGAGAGAGATG+TGG + chr3.3:86168997-86169016 MS.gene066525:CDS 40.0%
ATGTAGATGCTACCTTGGAT+TGG - chr3.3:86168802-86168821 None:intergenic 40.0%
CAAGTCAGAGGTTGAAATTC+TGG + chr3.3:86168725-86168744 MS.gene066525:CDS 40.0%
CAGAATTTCAACCTCTGACT+TGG - chr3.3:86168727-86168746 None:intergenic 40.0%
CAGCAATAAAGGTGAGAAGA+AGG + chr3.3:86168671-86168690 MS.gene066525:CDS 40.0%
GAACTACTATAGGTGTTCAG+TGG + chr3.3:86168958-86168977 MS.gene066525:CDS 40.0%
GAGGTTGAAATTCTGGATGA+TGG + chr3.3:86168732-86168751 MS.gene066525:CDS 40.0%
TACTATAGGTGTTCAGTGGA+TGG + chr3.3:86168962-86168981 MS.gene066525:CDS 40.0%
TGCATTCAAAACCAAGTCAG+AGG + chr3.3:86168713-86168732 MS.gene066525:CDS 40.0%
TGTAGATGCTACCTTGGATT+GGG - chr3.3:86168801-86168820 None:intergenic 40.0%
TTCACATGTAGATGCTACCT+TGG - chr3.3:86168807-86168826 None:intergenic 40.0%
!! AAATTCTGGATGATGGGTTC+AGG + chr3.3:86168739-86168758 MS.gene066525:CDS 40.0%
!! ATAGGAGGTGTCAGCAATAA+AGG + chr3.3:86168660-86168679 MS.gene066525:CDS 40.0%
ATGAGTATGTGTGCCTTCGT+AGG - chr3.3:86169047-86169066 None:intergenic 45.0%
GTAGATGCTACCTTGGATTG+GGG - chr3.3:86168800-86168819 None:intergenic 45.0%
TGAAAAACAGCCCCAATCCA+AGG + chr3.3:86168787-86168806 MS.gene066525:CDS 45.0%
! GAGGAAGTACGGAAAGAAGA+TGG + chr3.3:86168764-86168783 MS.gene066525:CDS 45.0%
!! TTCTGGATGATGGGTTCAGG+TGG + chr3.3:86168742-86168761 MS.gene066525:CDS 50.0%
GGGTTCAGGTGGAGGAAGTA+CGG + chr3.3:86168753-86168772 MS.gene066525:CDS 55.0%
!! TGGATGATGGGTTCAGGTGG+AGG + chr3.3:86168745-86168764 MS.gene066525:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 86168636 86169081 86168636 ID=MS.gene066525
chr3.3 mRNA 86168636 86169081 86168636 ID=MS.gene066525.t1;Parent=MS.gene066525
chr3.3 exon 86168636 86168808 86168636 ID=MS.gene066525.t1.exon1;Parent=MS.gene066525.t1
chr3.3 CDS 86168636 86168808 86168636 ID=cds.MS.gene066525.t1;Parent=MS.gene066525.t1
chr3.3 exon 86168958 86169081 86168958 ID=MS.gene066525.t1.exon2;Parent=MS.gene066525.t1
chr3.3 CDS 86168958 86169081 86168958 ID=cds.MS.gene066525.t1;Parent=MS.gene066525.t1
Gene Sequence

>MS.gene066525

ATGTCATTGGTTTGTGTCATATTTATAGGAGGTGTCAGCAATAAAGGTGAGAAGAAGGAAATTAGAGATCATAAAGTTGCATTCAAAACCAAGTCAGAGGTTGAAATTCTGGATGATGGGTTCAGGTGGAGGAAGTACGGAAAGAAGATGGTGAAAAACAGCCCCAATCCAAGGTAGCATCTACATGTGAATATGATATAGTTTAAGTCTAAGTATAAGTAAGATCTCTGTTTTAGAATTTATTTTAGATTGTTTCTAACATGAACCATCTCTTATATATTATGTATTTATGTTAGTTGAGAATTTCCAAATTTGTTTACAGGAACTACTATAGGTGTTCAGTGGATGGATGCCAAGTGAAAAAGAGAGTTGAGAGAGATGTGGACGATCCAAGTTATGTAATAACAACCTACGAAGGCACACATACTCATCCAAGTTTTCACTAG
Protein sequence

>MS.gene066525.t1

MSLVCVIFIGGVSNKGEKKEIRDHKVAFKTKSEVEILDDGFRWRKYGKKMVKNSPNPRNYYRCSVDGCQVKKRVERDVDDPSYVITTYEGTHTHPSFH