AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066682

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066682.t1 MTR_8g078410 95.000 40 2 0 108 147 160 199 5.21e-25 99.8
MS.gene066682.t1 MTR_5g018900 87.500 40 5 0 108 147 157 196 3.16e-22 91.7
MS.gene066682.t1 MTR_5g018910 58.571 70 22 2 78 147 474 536 1.31e-19 84.3
MS.gene066682.t1 MTR_1g035230 92.308 39 3 0 36 74 133 171 1.30e-17 78.6
MS.gene066682.t1 MTR_1g021835 72.500 40 11 0 108 147 110 149 2.93e-16 74.7
MS.gene066682.t1 MTR_1g021835 72.500 40 11 0 108 147 110 149 4.21e-16 74.3
MS.gene066682.t1 MTR_1g021835 72.500 40 11 0 108 147 110 149 4.30e-16 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066682.t1 AT4G36860 80.000 40 8 0 108 147 189 228 2.89e-20 86.3
MS.gene066682.t1 AT4G36860 80.000 40 8 0 108 147 213 252 2.94e-20 86.3
MS.gene066682.t1 AT4G36860 80.000 40 8 0 108 147 189 228 3.05e-20 86.3
MS.gene066682.t1 AT1G19270 65.000 40 14 0 108 147 171 210 1.74e-16 75.5
MS.gene066682.t1 AT1G19270 65.000 40 14 0 108 147 171 210 1.74e-16 75.5
MS.gene066682.t1 AT1G20950 80.000 40 8 0 36 75 152 191 6.40e-15 70.9
MS.gene066682.t1 AT1G76550 76.923 39 9 0 36 74 152 190 1.06e-13 67.4
MS.gene066682.t1 AT2G39830 55.814 43 19 0 105 147 158 200 1.20e-11 61.6
MS.gene066682.t1 AT2G39830 55.814 43 19 0 105 147 149 191 1.25e-11 61.6
MS.gene066682.t1 AT2G39830 55.814 43 19 0 105 147 158 200 1.27e-11 61.6
MS.gene066682.t1 AT2G39830 55.814 43 19 0 105 147 158 200 1.72e-11 61.2

Find 29 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCCTGGATCTTCATTTA+TGG 0.162017 4.3:+76086301 MS.gene066682:CDS
ATCGATTAGTTTGCAATGAT+TGG 0.253265 4.3:+76083945 None:intergenic
TGCAACAATTTGAAACTTGA+TGG 0.277323 4.3:+76086254 MS.gene066682:CDS
ATTGGAGGTATAATTCTTCC+TGG 0.347408 4.3:+76086287 MS.gene066682:CDS
AGGTATATGGATACCTCCTT+AGG 0.349000 4.3:-76084032 None:intergenic
CAGGTATCAGAGATCAGTTT+TGG 0.350700 4.3:+76084527 MS.gene066682:intron
GTCATCTTTCACATAGTTAC+CGG 0.377245 4.3:-76086357 None:intergenic
GGCGGACAAAAGATCAAATT+AGG 0.380499 4.3:+76086198 MS.gene066682:CDS
CATAGTTACCGGTTCTATCA+AGG 0.405748 4.3:-76086346 None:intergenic
TAGGTGGTTATGATTTGCTT+GGG 0.438309 4.3:+76086177 MS.gene066682:intron
GCTGGCTGCAATGCCGAGAT+TGG 0.440822 4.3:+76086631 MS.gene066682:CDS
TTAGGTGGTTATGATTTGCT+TGG 0.454502 4.3:+76086176 MS.gene066682:intron
CAAACCTCGTAATCAGTGAT+TGG 0.457824 4.3:-76086727 None:intergenic
TACAATTGATTTCAACCCTA+AGG 0.478843 4.3:+76084016 MS.gene066682:CDS
TGATTGGAAGTTTGCAAGCA+TGG 0.482821 4.3:-76086711 None:intergenic
TGAGTTGCATGGGAGCTGTA+TGG 0.499390 4.3:+76086668 MS.gene066682:CDS
TTGAGCTTGCTCGCCTCAAG+TGG 0.500004 4.3:+76083985 MS.gene066682:CDS
AAAACTTCCATGACCAATCT+CGG 0.503649 4.3:-76086644 None:intergenic
GGTATATGGATACCTCCTTA+GGG 0.518954 4.3:-76084031 None:intergenic
ATAGTTACCGGTTCTATCAA+GGG 0.547174 4.3:-76086345 None:intergenic
GTTGCCATAAATGAAGATCC+AGG 0.557665 4.3:-76086305 None:intergenic
TTGTAAATGTGCGCCACTTG+AGG 0.559876 4.3:-76083998 None:intergenic
GTATTTAGACTTACATACTA+CGG 0.578160 4.3:-76086388 None:intergenic
TATGCCTGTAGAATCTGCGC+TGG 0.583339 4.3:+76086613 MS.gene066682:intron
TGCAATGCCGAGATTGGTCA+TGG 0.599329 4.3:+76086637 MS.gene066682:CDS
AGCAACATTAACTTGTTCAG+TGG 0.610807 4.3:-76086222 None:intergenic
ACTTCCAATCACTGATTACG+AGG 0.616089 4.3:+76086723 MS.gene066682:CDS
AATTGATTTCAACCCTAAGG+AGG 0.622628 4.3:+76084019 MS.gene066682:CDS
GTGGTTATGATTTGCTTGGG+CGG 0.640052 4.3:+76086180 MS.gene066682:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAGTTAAAAACAAA+TGG - chr4.3:76084419-76084438 None:intergenic 10.0%
!! AAAGAAAAATAAAATTGCAA+TGG - chr4.3:76084293-76084312 None:intergenic 15.0%
!! TTGTTAAAATGTACAAAAAT+TGG + chr4.3:76084322-76084341 MS.gene066682:intron 15.0%
!!! TGCAATTTTATTTTTCTTTT+GGG + chr4.3:76084294-76084313 MS.gene066682:intron 15.0%
!!! TTGCAATTTTATTTTTCTTT+TGG + chr4.3:76084293-76084312 MS.gene066682:intron 15.0%
!!! TTTTAACTATTTTTTTTCAG+TGG + chr4.3:76084424-76084443 MS.gene066682:intron 15.0%
!! TAATATATTGTAAGCTTGAT+TGG - chr4.3:76085190-76085209 None:intergenic 20.0%
!! TATTCCAAAGTTATAAACAT+CGG - chr4.3:76084633-76084652 None:intergenic 20.0%
!! TCAAGCTTACAATATATTAA+AGG + chr4.3:76085191-76085210 MS.gene066682:intron 20.0%
!!! AAAGAAACTACATTAGTTTA+TGG + chr4.3:76084170-76084189 MS.gene066682:intron 20.0%
!!! AGTCTATGCTTTTATATATA+AGG - chr4.3:76086574-76086593 None:intergenic 20.0%
!!! ATAGTTTTTTGTAAAGCTTT+TGG - chr4.3:76085974-76085993 None:intergenic 20.0%
!!! GCAATTTTATTTTTCTTTTG+GGG + chr4.3:76084295-76084314 MS.gene066682:intron 20.0%
!!! TTAGTCAATCAATTATTTCT+TGG + chr4.3:76086110-76086129 MS.gene066682:intron 20.0%
!!! TTTCCATGATTTTACATATT+AGG - chr4.3:76086469-76086488 None:intergenic 20.0%
! AGACTATCTTAATACAACAA+AGG - chr4.3:76085656-76085675 None:intergenic 25.0%
! AGCTTTACAAAAAACTATGA+AGG + chr4.3:76085976-76085995 MS.gene066682:intron 25.0%
! ATAGTCATTCCTTAAGAAAA+TGG + chr4.3:76084911-76084930 MS.gene066682:intron 25.0%
! ATAGTCATTCCTTAAGAAAA+TGG - chr4.3:76084930-76084911 None:intergenic 25.0%
! CTATGATATTTGAGTCATTA+AGG - chr4.3:76086040-76086059 None:intergenic 25.0%
! CTTAAGAAAATGGTGAAATT+TGG + chr4.3:76084921-76084940 MS.gene066682:intron 25.0%
! GTATTTAGACTTACATACTA+CGG - chr4.3:76086391-76086410 None:intergenic 25.0%
! TACATTTATTGTTCGAAAGT+TGG - chr4.3:76084090-76084109 None:intergenic 25.0%
! TACGATGATTGTTCTTTATT+AGG + chr4.3:76086158-76086177 MS.gene066682:intron 25.0%
! TATTGTGCTTTATAGGTATA+TGG - chr4.3:76084048-76084067 None:intergenic 25.0%
! TGATGTAGAAAAGAAAAATC+AGG - chr4.3:76085946-76085965 None:intergenic 25.0%
! TGCAGATAAAATTCTTAGAA+AGG - chr4.3:76084765-76084784 None:intergenic 25.0%
! TGGTAAATATTGTGCTTTAT+AGG - chr4.3:76084055-76084074 None:intergenic 25.0%
!! ACTTCATCTGTTCAATATTT+TGG + chr4.3:76084375-76084394 MS.gene066682:intron 25.0%
!! ATAAAAGCATAGACTAAAAC+CGG + chr4.3:76086579-76086598 MS.gene066682:intron 25.0%
!! CAAATTTCACCATTTTCTTA+AGG + chr4.3:76085820-76085839 MS.gene066682:intron 25.0%
!! CAAATTTCACCATTTTCTTA+AGG - chr4.3:76085839-76085820 None:intergenic 25.0%
!!! CTAAAAAGTTTGCTTTTCAT+AGG + chr4.3:76086540-76086559 MS.gene066682:intron 25.0%
!!! GTCATTCTAAAATTGCAAAA+AGG + chr4.3:76084874-76084893 MS.gene066682:intron 25.0%
!!! GTCTTTCTAAAATTGCAAAA+AGG + chr4.3:76084852-76084871 MS.gene066682:intron 25.0%
!!! TTACAAATTTTTTTGAGCTC+CGG - chr4.3:76085627-76085646 None:intergenic 25.0%
AAAACGAAATTCTGTAGAGA+TGG + chr4.3:76085894-76085913 MS.gene066682:intron 30.0%
AAAATTGCAAAAAGGTCACT+GGG + chr4.3:76084882-76084901 MS.gene066682:intron 30.0%
AAAATTGGCAATGTTAATCG+AGG + chr4.3:76084337-76084356 MS.gene066682:intron 30.0%
AAAGTTGGCAAAATTAGAGT+TGG - chr4.3:76084075-76084094 None:intergenic 30.0%
AATTGCAATGGACATGAAAT+AGG - chr4.3:76084281-76084300 None:intergenic 30.0%
ATCGCTACATACGAAAAAAA+GGG + chr4.3:76086432-76086451 MS.gene066682:intron 30.0%
ATGTGAAAATAGTTCCATGT+TGG + chr4.3:76084798-76084817 MS.gene066682:intron 30.0%
CGAAAAAAAGGGAAAATGAT+TGG + chr4.3:76086443-76086462 MS.gene066682:intron 30.0%
GAAACCGATGTTTATAACTT+TGG + chr4.3:76084626-76084645 MS.gene066682:intron 30.0%
GATGATTGTTCTTTATTAGG+TGG + chr4.3:76086161-76086180 MS.gene066682:intron 30.0%
TAAAATTGCAAAAAGGTCAC+TGG + chr4.3:76084881-76084900 MS.gene066682:intron 30.0%
TAATCGAGGAATAAGACAAA+GGG + chr4.3:76084351-76084370 MS.gene066682:intron 30.0%
TCTTTAGCATATTCGATATG+TGG - chr4.3:76086413-76086432 None:intergenic 30.0%
TGGCCTAATATGTAAAATCA+TGG + chr4.3:76086463-76086482 MS.gene066682:intron 30.0%
TTAAAAACCCTTGATAGAAC+CGG + chr4.3:76086338-76086357 MS.gene066682:CDS 30.0%
TTAATCGAGGAATAAGACAA+AGG + chr4.3:76084350-76084369 MS.gene066682:intron 30.0%
TTGCATGTAGTCCATATAAA+TGG - chr4.3:76084216-76084235 None:intergenic 30.0%
TTTCGCATCAACCATTTATA+TGG + chr4.3:76084202-76084221 MS.gene066682:intron 30.0%
! AACTACATTAGTTTATGGAG+AGG + chr4.3:76084175-76084194 MS.gene066682:intron 30.0%
! AACTATTTTCACATGCAAGA+AGG - chr4.3:76084792-76084811 None:intergenic 30.0%
! ATTTTAGATCTCATCCAACA+TGG - chr4.3:76084815-76084834 None:intergenic 30.0%
! CTTTTTAGATGTGTACTATC+TGG - chr4.3:76086528-76086547 None:intergenic 30.0%
! TACAATTGATTTCAACCCTA+AGG + chr4.3:76084016-76084035 MS.gene066682:CDS 30.0%
! TGCAACAATTTGAAACTTGA+TGG + chr4.3:76086254-76086273 MS.gene066682:CDS 30.0%
!! AAGTTATAAACATCGGTTTC+AGG - chr4.3:76084626-76084645 None:intergenic 30.0%
!! AATTGTGAATTTTGCTGTCA+CGG + chr4.3:76085000-76085019 MS.gene066682:intron 30.0%
!! AATTGTGAATTTTGCTGTCA+CGG - chr4.3:76085019-76085000 None:intergenic 30.0%
!! TGCTTTCTAAAACTGCAAAA+AGG - chr4.3:76085869-76085888 None:intergenic 30.0%
!!! CTTGATGGTTTTGTCATTAT+TGG + chr4.3:76086269-76086288 MS.gene066682:CDS 30.0%
AAAACTGCAAAAAGGTCACT+GGG - chr4.3:76085861-76085880 None:intergenic 35.0%
AAAACTTCCATGACCAATCT+CGG - chr4.3:76086647-76086666 None:intergenic 35.0%
AACAAGGTCACCTTATGAAT+TGG + chr4.3:76084696-76084715 MS.gene066682:intron 35.0%
ACAAGGTCACCTTATGAATT+GGG + chr4.3:76084697-76084716 MS.gene066682:intron 35.0%
CACAATCTACTATTCTGTCA+TGG - chr4.3:76085057-76085076 None:intergenic 35.0%
CAGAAAATTGCAGAACTCTT+TGG + chr4.3:76085501-76085520 MS.gene066682:intron 35.0%
CGAAACTTACCCAATTCATA+AGG - chr4.3:76084709-76084728 None:intergenic 35.0%
GATCGCTACATACGAAAAAA+AGG + chr4.3:76086431-76086450 MS.gene066682:intron 35.0%
GTCATCTTTCACATAGTTAC+CGG - chr4.3:76086360-76086379 None:intergenic 35.0%
GTTAATGAGTAACGTGTATG+AGG + chr4.3:76084135-76084154 MS.gene066682:intron 35.0%
TAAAACTGCAAAAAGGTCAC+TGG - chr4.3:76085862-76085881 None:intergenic 35.0%
TCTTCCTGGATCTTCATTTA+TGG + chr4.3:76086301-76086320 MS.gene066682:CDS 35.0%
! AATTGATTTCAACCCTAAGG+AGG + chr4.3:76084019-76084038 MS.gene066682:CDS 35.0%
! ATTGCAGATTTTGAACTTGC+TGG - chr4.3:76085579-76085598 None:intergenic 35.0%
! ATTGGAGGTATAATTCTTCC+TGG + chr4.3:76086287-76086306 MS.gene066682:CDS 35.0%
! CTTAGAAAGGAGAGTTCTAA+AGG - chr4.3:76084752-76084771 None:intergenic 35.0%
! GATCTATTTTGTCACCATAG+AGG - chr4.3:76086506-76086525 None:intergenic 35.0%
!! AATTTGTTGTTCTGTCATGG+TGG + chr4.3:76085759-76085778 MS.gene066682:intron 35.0%
!! AATTTGTTGTTCTGTCATGG+TGG - chr4.3:76085778-76085759 None:intergenic 35.0%
!! AGCAACATTAACTTGTTCAG+TGG - chr4.3:76086225-76086244 None:intergenic 35.0%
!! ATAGTTACCGGTTCTATCAA+GGG - chr4.3:76086348-76086367 None:intergenic 35.0%
!! ATTGTGAATTTTGCTGTCAG+TGG + chr4.3:76085071-76085090 MS.gene066682:intron 35.0%
!! CACAATTTGTTGTTCTGTCA+TGG + chr4.3:76085756-76085775 MS.gene066682:intron 35.0%
!! CACAATTTGTTGTTCTGTCA+TGG - chr4.3:76085775-76085756 None:intergenic 35.0%
!! TAGGTGGTTATGATTTGCTT+GGG + chr4.3:76086177-76086196 MS.gene066682:intron 35.0%
!! TTAGGTGGTTATGATTTGCT+TGG + chr4.3:76086176-76086195 MS.gene066682:intron 35.0%
!!! GAGATCAGTTTTGGAAGTAA+TGG + chr4.3:76084536-76084555 MS.gene066682:intron 35.0%
!!! GATGGTTTTGTCATTATTGG+AGG + chr4.3:76086272-76086291 MS.gene066682:CDS 35.0%
!!! GGAAGTTTTTTGAGTTGCAT+GGG + chr4.3:76086658-76086677 MS.gene066682:CDS 35.0%
!!! TGGAAGTTTTTTGAGTTGCA+TGG + chr4.3:76086657-76086676 MS.gene066682:CDS 35.0%
AATTGAAATAGCCGCCTCTA+TGG + chr4.3:76086489-76086508 MS.gene066682:intron 40.0%
AGGTATATGGATACCTCCTT+AGG - chr4.3:76084035-76084054 None:intergenic 40.0%
CAAACCTCGTAATCAGTGAT+TGG - chr4.3:76086730-76086749 None:intergenic 40.0%
GAGTTTATGTGTTGTCATGG+TGG - chr4.3:76085122-76085141 None:intergenic 40.0%
GCATGGCAACAAAAACATTC+TGG - chr4.3:76086697-76086716 None:intergenic 40.0%
GCTGAGTTTATGTGTTGTCA+TGG - chr4.3:76085125-76085144 None:intergenic 40.0%
GGCGGACAAAAGATCAAATT+AGG + chr4.3:76086198-76086217 MS.gene066682:CDS 40.0%
GGTATATGGATACCTCCTTA+GGG - chr4.3:76084034-76084053 None:intergenic 40.0%
GTTGCCATAAATGAAGATCC+AGG - chr4.3:76086308-76086327 None:intergenic 40.0%
TATTAGCTAAGCGAGTCTGA+TGG + chr4.3:76085706-76085725 MS.gene066682:intron 40.0%
TGACCTTGTTCTTCAGAGTA+CGG - chr4.3:76084686-76084705 None:intergenic 40.0%
TGATTGGAAGTTTGCAAGCA+TGG - chr4.3:76086714-76086733 None:intergenic 40.0%
TTATAAACAGCCAGCACAGT+TGG + chr4.3:76084560-76084579 MS.gene066682:intron 40.0%
TTCTTCAGAATGAGATGCAG+TGG + chr4.3:76085535-76085554 MS.gene066682:intron 40.0%
! ACTTCCAATCACTGATTACG+AGG + chr4.3:76086723-76086742 MS.gene066682:CDS 40.0%
! CAGGCATATTTTGCAGTAAC+CGG - chr4.3:76086601-76086620 None:intergenic 40.0%
! CAGGTATCAGAGATCAGTTT+TGG + chr4.3:76084527-76084546 MS.gene066682:intron 40.0%
! CTATTTTGTCACCATAGAGG+CGG - chr4.3:76086503-76086522 None:intergenic 40.0%
! GCTGCGTTTGATTTCTATAC+CGG + chr4.3:76085605-76085624 MS.gene066682:intron 40.0%
! TGATACCTGAGAAAGTGCAA+TGG - chr4.3:76084516-76084535 None:intergenic 40.0%
!! AAAGGAGAGTTCTAAAGGAG+GGG - chr4.3:76084747-76084766 None:intergenic 40.0%
!! AGAAAGGAGAGTTCTAAAGG+AGG - chr4.3:76084749-76084768 None:intergenic 40.0%
!! CATAGTTACCGGTTCTATCA+AGG - chr4.3:76086349-76086368 None:intergenic 40.0%
!! GAAAGGAGAGTTCTAAAGGA+GGG - chr4.3:76084748-76084767 None:intergenic 40.0%
CTGTTCCATTGCACTTTCTC+AGG + chr4.3:76084508-76084527 MS.gene066682:intron 45.0%
TTGTAAATGTGCGCCACTTG+AGG - chr4.3:76084001-76084020 None:intergenic 45.0%
! CTACCGTACTCTGAAGAACA+AGG + chr4.3:76084680-76084699 MS.gene066682:intron 45.0%
!! AAACATCGGTTTCAGGACCT+TGG - chr4.3:76084619-76084638 None:intergenic 45.0%
!! AAGGAGAGTTCTAAAGGAGG+GGG - chr4.3:76084746-76084765 None:intergenic 45.0%
!! GTGGTTATGATTTGCTTGGG+CGG + chr4.3:76086180-76086199 MS.gene066682:CDS 45.0%
!! TGTGAATTTTGCTGTCACGG+TGG + chr4.3:76085003-76085022 MS.gene066682:intron 45.0%
!! TGTGAATTTTGCTGTCACGG+TGG - chr4.3:76085022-76085003 None:intergenic 45.0%
CAGTAGCTTAGCCACCAATC+TGG - chr4.3:76085794-76085813 None:intergenic 50.0%
TAGCTAAGCGAGTCTGATGG+TGG + chr4.3:76085709-76085728 MS.gene066682:intron 50.0%
TATGCCTGTAGAATCTGCGC+TGG + chr4.3:76086613-76086632 MS.gene066682:intron 50.0%
TGAGTTGCATGGGAGCTGTA+TGG + chr4.3:76086668-76086687 MS.gene066682:CDS 50.0%
TGATTCATCGCCAACTGTGC+TGG - chr4.3:76084573-76084592 None:intergenic 50.0%
! ATCGGTTTCAGGACCTTGGT+GGG - chr4.3:76084615-76084634 None:intergenic 50.0%
! TGCAATGCCGAGATTGGTCA+TGG + chr4.3:76086637-76086656 MS.gene066682:CDS 50.0%
!! AGAGTTCTAAAGGAGGGGGA+AGG - chr4.3:76084742-76084761 None:intergenic 50.0%
!! AGTTCTAAAGGAGGGGGAAG+GGG - chr4.3:76084740-76084759 None:intergenic 50.0%
!! GAGTTCTAAAGGAGGGGGAA+GGG - chr4.3:76084741-76084760 None:intergenic 50.0%
!! GGTGAAATTTGGTGTCGCAG+TGG + chr4.3:76084932-76084951 MS.gene066682:intron 50.0%
TCAGTGGCTTAGCCACCATC+TGG + chr4.3:76085087-76085106 MS.gene066682:intron 55.0%
TCGGTTTCAGGACCTTGGTG+GGG - chr4.3:76084614-76084633 None:intergenic 55.0%
TGACTAAGCGAGCCTGATGG+TGG - chr4.3:76085034-76085053 None:intergenic 55.0%
TGGTGACTAAGCGAGCCTGA+TGG - chr4.3:76085037-76085056 None:intergenic 55.0%
! TTGAGCTTGCTCGCCTCAAG+TGG + chr4.3:76083985-76084004 MS.gene066682:CDS 55.0%
!! CATCGGTTTCAGGACCTTGG+TGG - chr4.3:76084616-76084635 None:intergenic 55.0%
ACGGTGGCTTAGCCACCATC+AGG + chr4.3:76085019-76085038 MS.gene066682:intron 60.0%
GCAGCCAGCGCAGATTCTAC+AGG - chr4.3:76086620-76086639 None:intergenic 60.0%
GCTGGCTGCAATGCCGAGAT+TGG + chr4.3:76086631-76086650 MS.gene066682:CDS 60.0%
GGCTAAGCGAGCCAGATTGG+TGG + chr4.3:76085780-76085799 MS.gene066682:intron 60.0%
GGCTAAGCGAGCGAGATTGG+TGG - chr4.3:76084963-76084982 None:intergenic 60.0%
GGTGGCTAAGCGAGCCAGAT+TGG + chr4.3:76085777-76085796 MS.gene066682:intron 60.0%
GGTGGCTAAGCGAGCGAGAT+TGG - chr4.3:76084966-76084985 None:intergenic 60.0%
GTTTCAGGACCTTGGTGGGG+TGG - chr4.3:76084611-76084630 None:intergenic 60.0%
TCTGAGTTGCCACCCCACCA+AGG + chr4.3:76084599-76084618 MS.gene066682:intron 60.0%
TGGCTAAGCGAGCCAGATGG+TGG - chr4.3:76085102-76085121 None:intergenic 60.0%
TGGTGGCTAAGCGAGCCAGA+TGG - chr4.3:76085795-76085776 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 76083960 76086750 76083960 ID=MS.gene066682
chr4.3 mRNA 76083960 76086750 76083960 ID=MS.gene066682.t1;Parent=MS.gene066682
chr4.3 exon 76083960 76084040 76083960 ID=MS.gene066682.t1.exon1;Parent=MS.gene066682.t1
chr4.3 CDS 76083960 76084040 76083960 ID=cds.MS.gene066682.t1;Parent=MS.gene066682.t1
chr4.3 exon 76084530 76084551 76084530 ID=MS.gene066682.t1.exon2;Parent=MS.gene066682.t1
chr4.3 CDS 76084530 76084551 76084530 ID=cds.MS.gene066682.t1;Parent=MS.gene066682.t1
chr4.3 exon 76086180 76086396 76086180 ID=MS.gene066682.t1.exon3;Parent=MS.gene066682.t1
chr4.3 CDS 76086180 76086396 76086180 ID=cds.MS.gene066682.t1;Parent=MS.gene066682.t1
chr4.3 exon 76086624 76086750 76086624 ID=MS.gene066682.t1.exon4;Parent=MS.gene066682.t1
chr4.3 CDS 76086624 76086750 76086624 ID=cds.MS.gene066682.t1;Parent=MS.gene066682.t1
Gene Sequence

>MS.gene066682

ATGATTGGAGATGACAGTAACAGTCTTGAGCTTGCTCGCCTCAAGTGGCGCACATTTACAATTGATTTCAACCCTAAGGAGGTATCCATATACCTATAAAGCACAATATTTACCAACTCTAATTTTGCCAACTTTCGAACAATAAATGTATGAAAAATTTGTGATCTGCATGTTTGTTAATGAGTAACGTGTATGAGGCTCGAGCTTTGAAAAGAAACTACATTAGTTTATGGAGAGGAATATTTCGCATCAACCATTTATATGGACTACATGCAAACATGAAATTTATATTGCTCTAAAATGTTGTGTATAATTCTTCCTATTTCATGTCCATTGCAATTTTATTTTTCTTTTGGGGAAAATTGTTAAAATGTACAAAAATTGGCAATGTTAATCGAGGAATAAGACAAAGGGTACTTCATCTGTTCAATATTTTGGATTGTTTATTCGACTTATCCATTTGTTTTTAACTATTTTTTTTCAGTGGAAACTTGAATGATTTTGAGTAGTTTGCTTTTATCTTATGATGTATGTTATATAACAAATATCTGTTCCATTGCACTTTCTCAGGTATCAGAGATCAGTTTTGGAAGTAATGGATTATAAACAGCCAGCACAGTTGGCGATGAATCAAAGAAATCTGAGTTGCCACCCCACCAAGGTCCTGAAACCGATGTTTATAACTTTGGAATATTATTGCTTGAAATCATTTCTGCGAAACTACCGTACTCTGAAGAACAAGGTCACCTTATGAATTGGGTAAGTTTCGAATCTGCACCCCTTCCCCCTCCTTTAGAACTCTCCTTTCTAAGAATTTTATCTGCAACGTCCTTCTTGCATGTGAAAATAGTTCCATGTTGGATGAGATCTAAAATTTTAATATGATATTAGAGTCTTTCTAAAATTGCAAAAAGGTCATTCTAAAATTGCAAAAAGGTCACTGGGCAAAAAATAGTCATTCCTTAAGAAAATGGTGAAATTTGGTGTCGCAGTGGCTTAGCCACCAATCTCGCTCGCTTAGCCACCATGACAGAACAACAAATTGTGAATTTTGCTGTCACGGTGGCTTAGCCACCATCAGGCTCGCTTAGTCACCATGACAGAATAGTAGATTGTGAATTTTGCTGTCAGTGGCTTAGCCACCATCTGGCTCGCTTAGCCACCATGACAACACATAAACTCAGCAAAATCAACGAAATTCAAGCTTACAAACTCAAATACTTCAGTCCAATCAAGCTTACAATATATTAAAGGATTTCAAAATCTCTGCGACTACATCAGCATTGCAAAATGTTGAGTATACTTTTCTGATCTATAAGAGTTGAGTATACTTTTCTGATTTAGCACACACAACAAGTAACAAAATAGCTAGAACTGAAATTTTAGATGTTACAAATACACTTAAACTACATATTCAAGTAATGTCTAATATCTAAATATTTTATTTAACTTGAACATATGTTATTATTACTGAATTTAATACACTTTATTCTTATGCATCTATGTACATGTGAATTGAGTTTGCATCAAACTCACTTTTACAGAAAATTGCAGAACTCTTTGGTTAAGTCTTTGTTCTTCAGAATGAGATGCAGTGGAGTTTCTATCAAATTCAACCAGCAAGTTCAAAATCTGCAATATCGCAGCTGCGTTTGATTTCTATACCGGAGCTCAAAAAAATTTGTAATATTTACCTTTGTTGTATTAAGATAGTCTCTTGCTGATATTTTGATAAGATATATGCTATATTAGCTAAGCGAGTCTGATGGTGGCTAAGCCACCGTGACAGCAAAATTCACAATTTGTTGTTCTGTCATGGTGGCTAAGCGAGCCAGATTGGTGGCTAAGCTACTGTGACAACAAATTTCACCATTTTCTTAAGGAATGACTATTTTCTGCCCAGTGACCTTTTTGCAGTTTTAGAAAGCATTAATAAAACGAAATTCTGTAGAGATGGATAGATGACATCATACAATCGCTAATCCTGATTTTTCTTTTCTACATCATGCATCCAAAAGCTTTACAAAAAACTATGAAGGATACACAATTGTTTTATGAAAGTATAATGATTCATATTCCTTAATGACTCAAATATCATAGAAGTTACATGATTTTTATTTTTTAGTCTTTATTAATAATGCATGAAATTATTAGTCAATCAATTATTTCTTGGAAATTTTTTAACATTGTTATTATCATACGATGATTGTTCTTTATTAGGTGGTTATGATTTGCTTGGGCGGACAAAAGATCAAATTAGGACCACTGAACAAGTTAATGTTGCTCTTGCTACATGCAACAATTTGAAACTTGATGGTTTTGTCATTATTGGAGGTATAATTCTTCCTGGATCTTCATTTATGGCAACATTCACATCTTTAAAAACCCTTGATAGAACCGGTAACTATGTGAAAGATGACATCTCTATCCGTAGTATGTAAGTCTAAATACCACATATCGAATATGCTAAAGATCGCTACATACGAAAAAAAGGGAAAATGATTGGCCTAATATGTAAAATCATGGAAAAATTGAAATAGCCGCCTCTATGGTGACAAAATAGATCCAGATAGTACACATCTAAAAAGTTTGCTTTTCATAGGTTCTTGAACCTTATATATAAAAGCATAGACTAAAACCGGTTACTGCAAAATATGCCTGTAGAATCTGCGCTGGCTGCAATGCCGAGATTGGTCATGGAAGTTTTTTGAGTTGCATGGGAGCTGTATGGCATCCAGAATGTTTTTGTTGCCATGCTTGCAAACTTCCAATCACTGATTACGAGGTTTGA
Protein sequence

>MS.gene066682.t1

MIGDDSNSLELARLKWRTFTIDFNPKEVSEISFGSGYDLLGRTKDQIRTTEQVNVALATCNNLKLDGFVIIGGIILPGSSFMATFTSLKTLDRTGNYVKDDISIRSIICAGCNAEIGHGSFLSCMGAVWHPECFCCHACKLPITDYEV