AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066688

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066688.t1 MTR_4g022370 97.541 366 7 1 1 366 1 364 0.0 726
MS.gene066688.t1 MTR_7g059400 49.612 387 125 15 1 362 1 342 7.79e-88 269
MS.gene066688.t1 MTR_4g063780 44.669 347 138 15 1 325 1 315 4.13e-66 213
MS.gene066688.t1 MTR_2g096740 40.122 329 112 17 1 310 1 263 6.77e-55 182
MS.gene066688.t1 MTR_2g096740 38.217 314 124 16 1 310 1 248 1.43e-47 163
MS.gene066688.t1 MTR_7g024670 36.896 393 183 16 1 356 1 365 2.38e-47 165
MS.gene066688.t1 MTR_3g091820 63.636 88 28 1 51 138 16 99 3.15e-32 123
MS.gene066688.t1 MTR_4g088580 52.459 122 52 2 83 200 56 175 2.66e-31 122
MS.gene066688.t1 MTR_2g093220 80.328 61 12 0 76 136 34 94 4.60e-31 119
MS.gene066688.t1 MTR_8g027295 45.324 139 65 3 69 205 11 140 7.12e-31 118
MS.gene066688.t1 MTR_8g479350 51.351 111 49 2 82 188 68 177 9.31e-30 117
MS.gene066688.t1 MTR_5g031440 81.818 55 10 0 78 132 39 93 3.63e-29 115
MS.gene066688.t1 MTR_4g461080 65.385 78 26 1 57 134 5 81 3.70e-29 114
MS.gene066688.t1 MTR_8g015840 75.000 68 15 2 74 140 13 79 4.92e-29 112
MS.gene066688.t1 MTR_3g077750 63.855 83 27 2 50 132 32 111 6.02e-29 115
MS.gene066688.t1 MTR_3g090430 64.865 74 26 0 63 136 32 105 7.23e-29 114
MS.gene066688.t1 MTR_8g068210 69.841 63 19 0 79 141 29 91 2.23e-28 113
MS.gene066688.t1 MTR_8g079060 54.255 94 32 2 61 153 17 100 2.38e-28 110
MS.gene066688.t1 MTR_2g014170 86.275 51 7 0 81 131 44 94 3.14e-28 112
MS.gene066688.t1 MTR_1g056810 56.250 96 36 1 84 179 14 103 2.87e-27 109
MS.gene066688.t1 MTR_2g059540 86.000 50 7 0 81 130 30 79 4.78e-27 106
MS.gene066688.t1 MTR_1g077600 55.000 80 34 1 59 136 18 97 8.66e-27 107
MS.gene066688.t1 MTR_1g077600 55.000 80 34 1 59 136 18 97 1.08e-26 107
MS.gene066688.t1 MTR_4g109980 80.769 52 10 0 81 132 39 90 1.39e-26 108
MS.gene066688.t1 MTR_2g014060 66.667 72 19 2 62 133 10 76 3.25e-26 107
MS.gene066688.t1 MTR_4g089095 74.138 58 14 1 82 139 50 106 6.88e-26 105
MS.gene066688.t1 MTR_4g089095 74.138 58 14 1 82 139 16 72 7.55e-26 105
MS.gene066688.t1 MTR_8g044220 68.333 60 19 0 78 137 114 173 7.89e-26 108
MS.gene066688.t1 MTR_6g012450 67.213 61 20 0 78 138 132 192 2.54e-25 107
MS.gene066688.t1 MTR_2g013370 71.930 57 15 1 80 136 44 99 2.57e-25 103
MS.gene066688.t1 MTR_2g016030 70.175 57 17 0 79 135 44 100 6.56e-25 99.8
MS.gene066688.t1 MTR_6g027450 62.903 62 23 0 75 136 82 143 7.36e-25 103
MS.gene066688.t1 MTR_7g010950 62.500 64 24 0 75 138 128 191 1.21e-24 105
MS.gene066688.t1 MTR_4g082060 65.079 63 22 0 78 140 118 180 1.35e-24 104
MS.gene066688.t1 MTR_5g041420 41.509 106 57 1 33 138 94 194 2.72e-24 102
MS.gene066688.t1 MTR_7g086780 62.295 61 23 0 75 135 92 152 3.53e-24 103
MS.gene066688.t1 MTR_3g435480 63.492 63 23 0 78 140 132 194 4.90e-24 103
MS.gene066688.t1 MTR_6g027460 60.656 61 24 0 75 135 89 149 6.97e-24 101
MS.gene066688.t1 MTR_5g041380 53.247 77 36 0 66 142 111 187 1.01e-22 98.2
MS.gene066688.t1 MTR_5g041400 48.235 85 44 0 58 142 95 179 4.25e-22 96.7
MS.gene066688.t1 MTR_5g041530 45.977 87 44 1 52 135 107 193 7.47e-22 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066688.t1 AT3G55370 37.340 391 169 16 1 353 1 353 1.67e-54 183
MS.gene066688.t1 AT3G55370 42.321 293 116 12 1 278 1 255 3.02e-54 181
MS.gene066688.t1 AT2G37590 41.566 332 146 11 1 311 1 305 3.90e-50 171
MS.gene066688.t1 AT5G02460 39.096 376 144 13 1 315 1 352 4.58e-48 167
MS.gene066688.t1 AT3G55370 45.455 242 94 11 45 278 89 300 4.27e-47 164
MS.gene066688.t1 AT2G28810 34.409 372 176 15 1 345 1 331 6.47e-38 139
MS.gene066688.t1 AT1G07640 50.694 144 65 3 5 142 2 145 5.55e-37 136
MS.gene066688.t1 AT5G60200 57.407 108 45 1 78 184 51 158 1.71e-36 133
MS.gene066688.t1 AT1G07640 53.731 134 56 3 15 142 4 137 2.07e-36 135
MS.gene066688.t1 AT1G07640 70.886 79 23 0 64 142 3 81 3.83e-36 132
MS.gene066688.t1 AT2G28810 34.593 344 164 12 23 345 11 314 1.45e-35 132
MS.gene066688.t1 AT3G45610 55.238 105 44 2 78 181 36 138 4.65e-34 126
MS.gene066688.t1 AT5G65590 38.235 238 102 8 42 249 8 230 2.07e-32 124
MS.gene066688.t1 AT1G51700 60.000 95 34 3 77 167 27 121 8.96e-31 116
MS.gene066688.t1 AT1G28310 79.310 58 12 0 82 139 27 84 3.52e-30 117
MS.gene066688.t1 AT2G28510 81.967 61 11 0 81 141 47 107 3.99e-30 117
MS.gene066688.t1 AT1G28310 79.310 58 12 0 82 139 51 108 5.25e-30 117
MS.gene066688.t1 AT5G60850 71.014 69 20 0 69 137 38 106 1.69e-29 115
MS.gene066688.t1 AT3G61850 75.410 61 15 0 78 138 58 118 6.76e-29 114
MS.gene066688.t1 AT3G61850 75.410 61 15 0 78 138 58 118 6.76e-29 114
MS.gene066688.t1 AT3G61850 75.410 61 15 0 78 138 70 130 7.24e-29 114
MS.gene066688.t1 AT3G21270 84.906 53 8 0 82 134 29 81 8.22e-29 111
MS.gene066688.t1 AT3G61850 75.410 61 15 0 78 138 58 118 9.38e-29 114
MS.gene066688.t1 AT3G61850 75.410 61 15 0 78 138 70 130 1.18e-28 114
MS.gene066688.t1 AT3G52440 58.140 86 36 0 79 164 22 107 4.75e-28 110
MS.gene066688.t1 AT4G38000 73.438 64 16 1 76 138 35 98 4.79e-28 110
MS.gene066688.t1 AT3G52440 58.140 86 36 0 79 164 41 126 8.31e-28 110
MS.gene066688.t1 AT5G66940 48.958 96 38 2 63 147 2 97 4.05e-27 107
MS.gene066688.t1 AT1G21340 68.750 64 20 0 80 143 36 99 8.24e-27 107
MS.gene066688.t1 AT5G62940 86.000 50 7 0 83 132 74 123 1.73e-26 108
MS.gene066688.t1 AT2G34140 51.685 89 40 1 47 135 26 111 2.18e-26 103
MS.gene066688.t1 AT3G50410 69.492 59 18 0 82 140 30 88 3.12e-26 105
MS.gene066688.t1 AT4G00940 71.186 59 17 0 78 136 64 122 4.90e-26 106
MS.gene066688.t1 AT4G00940 71.186 59 17 0 78 136 64 122 4.90e-26 106
MS.gene066688.t1 AT4G00940 71.186 59 17 0 78 136 64 122 4.90e-26 106
MS.gene066688.t1 AT2G46590 78.846 52 11 0 78 129 64 115 2.17e-25 105
MS.gene066688.t1 AT2G46590 78.846 52 11 0 78 129 76 127 2.53e-25 105
MS.gene066688.t1 AT4G21050 66.129 62 21 0 75 136 17 78 5.08e-25 101
MS.gene066688.t1 AT1G29160 68.966 58 18 0 78 135 58 115 5.63e-25 100
MS.gene066688.t1 AT3G47500 64.062 64 23 0 75 138 103 166 1.06e-24 105
MS.gene066688.t1 AT1G64620 74.545 55 14 0 76 130 43 97 1.31e-24 103
MS.gene066688.t1 AT1G26790 55.072 69 31 0 67 135 96 164 4.04e-24 102
MS.gene066688.t1 AT1G69570 62.295 61 23 0 75 135 125 185 5.95e-24 102
MS.gene066688.t1 AT5G62430 58.209 67 28 0 75 141 47 113 1.32e-23 99.8
MS.gene066688.t1 AT5G39660 62.903 62 23 0 74 135 130 191 1.38e-23 102
MS.gene066688.t1 AT5G39660 62.903 62 23 0 74 135 130 191 1.38e-23 102
MS.gene066688.t1 AT5G39660 62.903 62 23 0 74 135 130 191 1.38e-23 102
MS.gene066688.t1 AT1G47655 68.333 60 17 1 73 130 18 77 1.87e-23 97.1
MS.gene066688.t1 AT4G21040 50.562 89 40 1 84 172 27 111 4.37e-22 94.0
MS.gene066688.t1 AT4G21080 47.674 86 41 1 84 169 27 108 8.94e-21 90.9
MS.gene066688.t1 AT4G21030 55.932 59 23 1 71 129 13 68 3.03e-14 71.2

Find 100 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAGGAAGTGAACAATTT+TGG 0.154411 4.3:-76154599 MS.gene066688:CDS
AATTGGACTGATCATGTTTC+TGG 0.157222 4.3:-76154543 MS.gene066688:CDS
TCAACTTGCTTCTGTGAAAA+TGG 0.216358 4.3:-76154676 MS.gene066688:CDS
CCAGAAGTTGAAATCACTTT+TGG 0.251474 4.3:+76154708 None:intergenic
GCAAGTCTTGCAGAAGTGTC+TGG 0.259253 4.3:+76155268 None:intergenic
CCAAAAGTGATTTCAACTTC+TGG 0.271253 4.3:-76154708 MS.gene066688:CDS
GGTGATTTGGGAAGTGCTTT+AGG 0.279369 4.3:-76154909 MS.gene066688:CDS
GGAAGAGTGGATATTGTTAT+TGG 0.289267 4.3:+76155079 None:intergenic
ATGGGAGGAGTAGGTGATTT+GGG 0.291520 4.3:-76154921 MS.gene066688:CDS
GAGACGGAGCCGCCGTTTCC+TGG 0.321013 4.3:+76155503 None:intergenic
CATGGGAGGAGTAGGTGATT+TGG 0.324852 4.3:-76154922 MS.gene066688:CDS
TGGAATAGTGCTGTTGTTAC+TGG 0.330687 4.3:-76154579 MS.gene066688:CDS
TCCCGGTTGGTGGAGGCTTC+CGG 0.334717 4.3:-76155208 MS.gene066688:CDS
CTGGTAATTCTGCAGCTAAT+TGG 0.337179 4.3:-76154560 MS.gene066688:CDS
GGACAAGAGGCGGCGCTCTA+AGG 0.351075 4.3:-76155235 MS.gene066688:CDS
CCGACGACGACGAAATCATT+AGG 0.368177 4.3:+76154981 None:intergenic
CTCCGGAAGCCTCCACCAAC+CGG 0.381217 4.3:+76155206 None:intergenic
GGAGATTTAGGGTTAAACTA+TGG 0.382883 4.3:-76154954 MS.gene066688:CDS
GTCGTCGGAGGAGGAGATTT+AGG 0.386239 4.3:-76154966 MS.gene066688:CDS
CACTCTTCCGCCGATATATT+AGG 0.387275 4.3:-76155065 MS.gene066688:CDS
TCGTCGGAGGAGGAGATTTA+GGG 0.387617 4.3:-76154965 MS.gene066688:CDS
GCCCTATCGGACATCGATCC+TGG 0.393185 4.3:+76155395 None:intergenic
AATGGAAGATAGTATCAATA+GGG 0.409067 4.3:-76154658 MS.gene066688:CDS
TGCTTGATCTCTTGTTTCTC+CGG 0.410783 4.3:+76155189 None:intergenic
CTTCAAGTGGTTTGGAGCAA+TGG 0.413375 4.3:-76154869 MS.gene066688:CDS
TTGAACTAGCACTCCCCGTT+TGG 0.413567 4.3:+76155117 None:intergenic
AAATGGAAGATAGTATCAAT+AGG 0.414206 4.3:-76154659 MS.gene066688:CDS
CTTATCTTGATGCAGCCAAC+TGG 0.417162 4.3:-76155802 MS.gene066688:CDS
GCTAGCTCTGACCGCCAAAC+GGG 0.425727 4.3:-76155131 MS.gene066688:CDS
ACACTATAAAATTCTACATC+TGG 0.437923 4.3:+76154490 None:intergenic
TGTGACTGTGGTGGTGGTGG+TGG 0.440507 4.3:+76155458 None:intergenic
GCAAGACTTGCAGAAGGTAC+TGG 0.454376 4.3:-76155256 MS.gene066688:CDS
TGCAGCCAACTGGCAGCAAC+AGG 0.457644 4.3:-76155792 MS.gene066688:intron
CGACGACGACGAAATCATTA+GGG 0.464276 4.3:+76154982 None:intergenic
CTAAGGAATGTCCCGGTTGG+TGG 0.464766 4.3:-76155218 MS.gene066688:CDS
GAGGATCCACCGGCTCAATT+AGG 0.467697 4.3:-76155418 MS.gene066688:CDS
GTTCGCCATCCTTGCCCTAT+CGG 0.470894 4.3:+76155382 None:intergenic
TTTGAACATTTCCTTCAAAA+GGG 0.473490 4.3:+76154766 None:intergenic
AACTAGCACTCCCCGTTTGG+CGG 0.478302 4.3:+76155120 None:intergenic
GATTTCGTCGTCGTCGGAGG+AGG 0.481714 4.3:-76154975 MS.gene066688:CDS
AGGTGTAAGGCCTAATATAT+CGG 0.483491 4.3:+76155055 None:intergenic
GGCTAGCTCTGACCGCCAAA+CGG 0.486093 4.3:-76155132 MS.gene066688:CDS
GGAAACAATAACAGTAATCA+AGG 0.492146 4.3:-76154615 MS.gene066688:CDS
TTTAGTTACATGGGAGGAGT+AGG 0.502079 4.3:-76154930 MS.gene066688:CDS
CAAGTCTTGCAGAAGTGTCT+GGG 0.504581 4.3:+76155269 None:intergenic
GACTGTGACTGTGGTGGTGG+TGG 0.506110 4.3:+76155455 None:intergenic
AGGAATGTCCCGGTTGGTGG+AGG 0.511348 4.3:-76155215 MS.gene066688:CDS
GGACATTTCAGTGCTGCCTC+CGG 0.520025 4.3:+76155350 None:intergenic
AGAGTGGATATTGTTATTGG+AGG 0.520652 4.3:+76155082 None:intergenic
TTCAGTGCTGCCTCCGGCAT+AGG 0.523439 4.3:+76155356 None:intergenic
TTACTGAAGTTATGATGTAA+AGG 0.534579 4.3:+76155005 None:intergenic
ATCAAGATAAGCTGCTGGTA+TGG 0.536728 4.3:+76155814 None:intergenic
ATTTACCTGTTGCTGCCAGT+TGG 0.539661 4.3:+76155787 None:intergenic
GATGGCGAACATGCCTATGC+CGG 0.544192 4.3:-76155369 MS.gene066688:CDS
CATCATCAGCAACCAGGAAA+CGG 0.545159 4.3:-76155515 MS.gene066688:CDS
CACCTCAAATGTCATCATTG+AGG 0.546296 4.3:-76155037 MS.gene066688:CDS
CCTAATGATTTCGTCGTCGT+CGG 0.550564 4.3:-76154981 MS.gene066688:CDS
CGATCCTGGCCTAATTGAGC+CGG 0.550851 4.3:+76155409 None:intergenic
TGGAAACTCAACAAAGTCTC+CGG 0.552418 4.3:-76155153 MS.gene066688:CDS
TGAGACTGTGACTGTGGTGG+TGG 0.553445 4.3:+76155452 None:intergenic
AATGATTTCGTCGTCGTCGG+AGG 0.556123 4.3:-76154978 MS.gene066688:CDS
CAAGTGGTTTGGAGCAATGG+AGG 0.557508 4.3:-76154866 MS.gene066688:CDS
CCTCATGGAGGAGGATCCAC+CGG 0.558615 4.3:-76155428 MS.gene066688:CDS
ATGATGACATTTGAGGTGTA+AGG 0.560208 4.3:+76155042 None:intergenic
CAGTCACAGTCTCAACCTCA+TGG 0.560556 4.3:-76155443 MS.gene066688:CDS
GCCAGGATCGATGTCCGATA+GGG 0.563470 4.3:-76155396 MS.gene066688:CDS
TCAAGCAAAAGTAGCAACAA+TGG 0.567762 4.3:-76155173 MS.gene066688:CDS
TCCTGGCCTAATTGAGCCGG+TGG 0.569726 4.3:+76155412 None:intergenic
CAGAATCATCATCAGCAACC+AGG 0.572861 4.3:-76155521 MS.gene066688:CDS
GATGTCCGATAGGGCAAGGA+TGG 0.573727 4.3:-76155387 MS.gene066688:CDS
AACCTCAATGATGACATTTG+AGG 0.576269 4.3:+76155035 None:intergenic
CATGAAATGATAACTAGTGG+TGG 0.580805 4.3:-76154741 MS.gene066688:CDS
CTAATATATCGGCGGAAGAG+TGG 0.581651 4.3:+76155066 None:intergenic
TCCGGAAGCCTCCACCAACC+GGG 0.583043 4.3:+76155207 None:intergenic
TCCACCGGCTCAATTAGGCC+AGG 0.583672 4.3:-76155413 MS.gene066688:CDS
GATTTGGGAAGTGCTTTAGG+AGG 0.585086 4.3:-76154906 MS.gene066688:CDS
CGGCGCTCTAAGGAATGTCC+CGG 0.585823 4.3:-76155225 MS.gene066688:CDS
GGCCAGGATCGATGTCCGAT+AGG 0.588624 4.3:-76155397 MS.gene066688:CDS
CAGTCTCAACCTCATGGAGG+AGG 0.588847 4.3:-76155437 MS.gene066688:CDS
CTGAAGCAGTTGTTGAGAGA+CGG 0.593094 4.3:+76155487 None:intergenic
GGCGAACATGCCTATGCCGG+AGG 0.593250 4.3:-76155366 MS.gene066688:CDS
GCTGCATCAAGATAAGCTGC+TGG 0.594629 4.3:+76155809 None:intergenic
GCTCTAAGGAATGTCCCGGT+TGG 0.597092 4.3:-76155221 MS.gene066688:CDS
GTTTGGCGGTCAGAGCTAGC+CGG 0.603255 4.3:+76155134 None:intergenic
TGCAGAAGGTACTGGACAAG+AGG 0.603431 4.3:-76155248 MS.gene066688:CDS
AATCATGAAATGATAACTAG+TGG 0.606438 4.3:-76154744 MS.gene066688:CDS
TGAGGTTGAGACTGTGACTG+TGG 0.613393 4.3:+76155446 None:intergenic
AGAAGGTACTGGACAAGAGG+CGG 0.614306 4.3:-76155245 MS.gene066688:CDS
GATCGATGTCCGATAGGGCA+AGG 0.614474 4.3:-76155391 MS.gene066688:CDS
CTAGCTCTGACCGCCAAACG+GGG 0.632683 4.3:-76155130 MS.gene066688:CDS
ATGGAAGATAGTATCAATAG+GGG 0.643018 4.3:-76154657 MS.gene066688:CDS
TGTTGATGAGTCATTGACAT+TGG 0.657135 4.3:+76154840 None:intergenic
CATCAGCAACCAGGAAACGG+CGG 0.659295 4.3:-76155512 MS.gene066688:CDS
ACTTCTGCAAGACTTGCAGA+AGG 0.679196 4.3:-76155262 MS.gene066688:CDS
GGTTGAGACTGTGACTGTGG+TGG 0.680166 4.3:+76155449 None:intergenic
CCGGTGGATCCTCCTCCATG+AGG 0.687789 4.3:+76155428 None:intergenic
TGTAAGGCCTAATATATCGG+CGG 0.708601 4.3:+76155058 None:intergenic
TAACTAGTGGTGGATATGTG+AGG 0.730675 4.3:-76154731 MS.gene066688:CDS
GTGTTGGTTGATTCACAACG+TGG 0.731087 4.3:+76155329 None:intergenic
TCACAGTCTCAACCTCATGG+AGG 0.766876 4.3:-76155440 MS.gene066688:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTCAAAAGATAAAA+AGG - chr4.3:76154626-76154645 MS.gene066688:CDS 10.0%
!! TAAGAAAGAAATCAAATATT+TGG + chr4.3:76154704-76154723 None:intergenic 15.0%
!! AACAACATAAATAGACAAAA+AGG + chr4.3:76154779-76154798 None:intergenic 20.0%
!! AGTATATGAATTGAGAATAA+GGG + chr4.3:76154738-76154757 None:intergenic 20.0%
!!! AATTCAATTTTATCTTCAAG+TGG - chr4.3:76155448-76155467 MS.gene066688:CDS 20.0%
!!! TTTTGAATTATTTTGCCTTT+TGG + chr4.3:76154619-76154638 None:intergenic 20.0%
! AAAGCAAGAACCAAATTAAA+TGG + chr4.3:76154585-76154604 None:intergenic 25.0%
! AAATGGAAGATAGTATCAAT+AGG - chr4.3:76155671-76155690 MS.gene066688:intron 25.0%
! AATCATGAAATGATAACTAG+TGG - chr4.3:76155586-76155605 MS.gene066688:intron 25.0%
! AATGGAAGATAGTATCAATA+GGG - chr4.3:76155672-76155691 MS.gene066688:intron 25.0%
! ACAGGTAAATATAATTAGCA+AGG - chr4.3:76154556-76154575 MS.gene066688:CDS 25.0%
! GAGTATATGAATTGAGAATA+AGG + chr4.3:76154739-76154758 None:intergenic 25.0%
! TATTTGGTATATTCAAGAAG+AGG + chr4.3:76154688-76154707 None:intergenic 25.0%
! TTACTGAAGTTATGATGTAA+AGG + chr4.3:76155328-76155347 None:intergenic 25.0%
! TTTGAACATTTCCTTCAAAA+GGG + chr4.3:76155567-76155586 None:intergenic 25.0%
!! AATTTTATCTTCAAGTGGTT+TGG - chr4.3:76155453-76155472 MS.gene066688:CDS 25.0%
!! GAAGTAACAAAATTTTGTGT+TGG + chr4.3:76155020-76155039 None:intergenic 25.0%
!! TTTTGAACATTTCCTTCAAA+AGG + chr4.3:76155568-76155587 None:intergenic 25.0%
!!! AAACTATGGTTTTAGTTACA+TGG - chr4.3:76155390-76155409 MS.gene066688:CDS 25.0%
!!! AACTATGGTTTTAGTTACAT+GGG - chr4.3:76155391-76155410 MS.gene066688:CDS 25.0%
!!! ATTTCCTTTTTTGGCTAATT+TGG - chr4.3:76155519-76155538 MS.gene066688:CDS 25.0%
AATTGAGAATAAGGGAAGAA+AGG + chr4.3:76154730-76154749 None:intergenic 30.0%
AGCAAGGTTTCCATTTAATT+TGG - chr4.3:76154572-76154591 MS.gene066688:CDS 30.0%
ATGGAAGATAGTATCAATAG+GGG - chr4.3:76155673-76155692 MS.gene066688:intron 30.0%
ATTGAGAATAAGGGAAGAAA+GGG + chr4.3:76154729-76154748 None:intergenic 30.0%
GGAAACAATAACAGTAATCA+AGG - chr4.3:76155715-76155734 MS.gene066688:intron 30.0%
! ATCAAGGAAGTGAACAATTT+TGG - chr4.3:76155731-76155750 MS.gene066688:intron 30.0%
!! AGTAATTTGTACCCTTTTGA+AGG - chr4.3:76155553-76155572 MS.gene066688:intron 30.0%
!! CTTTTGTTTCTTTGTCCAAA+AGG - chr4.3:76154601-76154620 MS.gene066688:CDS 30.0%
!!! ACAGCATCAATTTCCTTTTT+TGG - chr4.3:76155510-76155529 MS.gene066688:CDS 30.0%
AACCTCAATGATGACATTTG+AGG + chr4.3:76155298-76155317 None:intergenic 35.0%
AATTGGACTGATCATGTTTC+TGG - chr4.3:76155787-76155806 MS.gene066688:intron 35.0%
AGGTGTAAGGCCTAATATAT+CGG + chr4.3:76155278-76155297 None:intergenic 35.0%
CATGAAATGATAACTAGTGG+TGG - chr4.3:76155589-76155608 MS.gene066688:intron 35.0%
CCAAAAGTGATTTCAACTTC+TGG - chr4.3:76155622-76155641 MS.gene066688:intron 35.0%
GGAAGAGTGGATATTGTTAT+TGG + chr4.3:76155254-76155273 None:intergenic 35.0%
TCAAGCAAAAGTAGCAACAA+TGG - chr4.3:76155157-76155176 MS.gene066688:CDS 35.0%
! AGAGTGGATATTGTTATTGG+AGG + chr4.3:76155251-76155270 None:intergenic 35.0%
! AGTATCAATAGGGGCTTTTT+GGG - chr4.3:76155682-76155701 MS.gene066688:intron 35.0%
! ATGATGACATTTGAGGTGTA+AGG + chr4.3:76155291-76155310 None:intergenic 35.0%
! CCAGAAGTTGAAATCACTTT+TGG + chr4.3:76155625-76155644 None:intergenic 35.0%
! GGAGATTTAGGGTTAAACTA+TGG - chr4.3:76155376-76155395 MS.gene066688:CDS 35.0%
! TAGTATCAATAGGGGCTTTT+TGG - chr4.3:76155681-76155700 MS.gene066688:intron 35.0%
! TCAACTTGCTTCTGTGAAAA+TGG - chr4.3:76155654-76155673 MS.gene066688:intron 35.0%
! TGTTGATGAGTCATTGACAT+TGG + chr4.3:76155493-76155512 None:intergenic 35.0%
!!! CCTTTTTTGGCTAATTTGGA+GGG - chr4.3:76155523-76155542 MS.gene066688:CDS 35.0%
!!! TATGGTTTTAGTTACATGGG+AGG - chr4.3:76155394-76155413 MS.gene066688:CDS 35.0%
!!! TCCTTTTTTGGCTAATTTGG+AGG - chr4.3:76155522-76155541 MS.gene066688:CDS 35.0%
ATCAAGATAAGCTGCTGGTA+TGG + chr4.3:76154519-76154538 None:intergenic 40.0%
CACCTCAAATGTCATCATTG+AGG - chr4.3:76155293-76155312 MS.gene066688:CDS 40.0%
CCCTCCAAATTAGCCAAAAA+AGG + chr4.3:76155526-76155545 None:intergenic 40.0%
CTGGTAATTCTGCAGCTAAT+TGG - chr4.3:76155770-76155789 MS.gene066688:intron 40.0%
TGGAAACTCAACAAAGTCTC+CGG - chr4.3:76155177-76155196 MS.gene066688:CDS 40.0%
TGTAAGGCCTAATATATCGG+CGG + chr4.3:76155275-76155294 None:intergenic 40.0%
TTTAGTTACATGGGAGGAGT+AGG - chr4.3:76155400-76155419 MS.gene066688:CDS 40.0%
! TGCTTGATCTCTTGTTTCTC+CGG + chr4.3:76155144-76155163 None:intergenic 40.0%
! TGGAATAGTGCTGTTGTTAC+TGG - chr4.3:76155751-76155770 MS.gene066688:intron 40.0%
!! TAACTAGTGGTGGATATGTG+AGG - chr4.3:76155599-76155618 MS.gene066688:intron 40.0%
ACTTCTGCAAGACTTGCAGA+AGG - chr4.3:76155068-76155087 MS.gene066688:CDS 45.0%
ATGGGAGGAGTAGGTGATTT+GGG - chr4.3:76155409-76155428 MS.gene066688:CDS 45.0%
ATTTACCTGTTGCTGCCAGT+TGG + chr4.3:76154546-76154565 None:intergenic 45.0%
CACTCTTCCGCCGATATATT+AGG - chr4.3:76155265-76155284 MS.gene066688:CDS 45.0%
CAGAATCATCATCAGCAACC+AGG - chr4.3:76154809-76154828 MS.gene066688:CDS 45.0%
CATCATCAGCAACCAGGAAA+CGG - chr4.3:76154815-76154834 MS.gene066688:CDS 45.0%
CCTAATGATTTCGTCGTCGT+CGG - chr4.3:76155349-76155368 MS.gene066688:CDS 45.0%
CGACGACGACGAAATCATTA+GGG + chr4.3:76155351-76155370 None:intergenic 45.0%
CTAATATATCGGCGGAAGAG+TGG + chr4.3:76155267-76155286 None:intergenic 45.0%
CTGAAGCAGTTGTTGAGAGA+CGG + chr4.3:76154846-76154865 None:intergenic 45.0%
CTTATCTTGATGCAGCCAAC+TGG - chr4.3:76154528-76154547 MS.gene066688:CDS 45.0%
! CTTCAAGTGGTTTGGAGCAA+TGG - chr4.3:76155461-76155480 MS.gene066688:CDS 45.0%
!! CAAGTCTTGCAGAAGTGTCT+GGG + chr4.3:76155064-76155083 None:intergenic 45.0%
!! GATTTGGGAAGTGCTTTAGG+AGG - chr4.3:76155424-76155443 MS.gene066688:CDS 45.0%
!! GGCTTTTTGGGAATTAGCAC+AGG - chr4.3:76155694-76155713 MS.gene066688:intron 45.0%
!! GGTGATTTGGGAAGTGCTTT+AGG - chr4.3:76155421-76155440 MS.gene066688:CDS 45.0%
!! GTGTTGGTTGATTCACAACG+TGG + chr4.3:76155004-76155023 None:intergenic 45.0%
AATGATTTCGTCGTCGTCGG+AGG - chr4.3:76155352-76155371 MS.gene066688:CDS 50.0%
CAGTCACAGTCTCAACCTCA+TGG - chr4.3:76154887-76154906 MS.gene066688:CDS 50.0%
CATGGGAGGAGTAGGTGATT+TGG - chr4.3:76155408-76155427 MS.gene066688:CDS 50.0%
CCGACGACGACGAAATCATT+AGG + chr4.3:76155352-76155371 None:intergenic 50.0%
GCTGCATCAAGATAAGCTGC+TGG + chr4.3:76154524-76154543 None:intergenic 50.0%
TCACAGTCTCAACCTCATGG+AGG - chr4.3:76154890-76154909 MS.gene066688:CDS 50.0%
TCGTCGGAGGAGGAGATTTA+GGG - chr4.3:76155365-76155384 MS.gene066688:CDS 50.0%
TGAGGTTGAGACTGTGACTG+TGG + chr4.3:76154887-76154906 None:intergenic 50.0%
TTGAACTAGCACTCCCCGTT+TGG + chr4.3:76155216-76155235 None:intergenic 50.0%
! CAAGTGGTTTGGAGCAATGG+AGG - chr4.3:76155464-76155483 MS.gene066688:CDS 50.0%
! GCAAGACTTGCAGAAGGTAC+TGG - chr4.3:76155074-76155093 MS.gene066688:CDS 50.0%
!! AGAAGGTACTGGACAAGAGG+CGG - chr4.3:76155085-76155104 MS.gene066688:CDS 50.0%
!! GCAAGTCTTGCAGAAGTGTC+TGG + chr4.3:76155065-76155084 None:intergenic 50.0%
!! TGCAGAAGGTACTGGACAAG+AGG - chr4.3:76155082-76155101 MS.gene066688:CDS 50.0%
AACTAGCACTCCCCGTTTGG+CGG + chr4.3:76155213-76155232 None:intergenic 55.0%
CAGTCTCAACCTCATGGAGG+AGG - chr4.3:76154893-76154912 MS.gene066688:CDS 55.0%
CATCAGCAACCAGGAAACGG+CGG - chr4.3:76154818-76154837 MS.gene066688:CDS 55.0%
CGATCCTGGCCTAATTGAGC+CGG + chr4.3:76154924-76154943 None:intergenic 55.0%
CTAAGGAATGTCCCGGTTGG+TGG - chr4.3:76155112-76155131 MS.gene066688:CDS 55.0%
GATCGATGTCCGATAGGGCA+AGG - chr4.3:76154939-76154958 MS.gene066688:CDS 55.0%
GATGGCGAACATGCCTATGC+CGG - chr4.3:76154961-76154980 MS.gene066688:CDS 55.0%
GATGTCCGATAGGGCAAGGA+TGG - chr4.3:76154943-76154962 MS.gene066688:CDS 55.0%
GCCAGGATCGATGTCCGATA+GGG - chr4.3:76154934-76154953 MS.gene066688:CDS 55.0%
GGACATTTCAGTGCTGCCTC+CGG + chr4.3:76154983-76155002 None:intergenic 55.0%
GGTTGAGACTGTGACTGTGG+TGG + chr4.3:76154884-76154903 None:intergenic 55.0%
GTCGTCGGAGGAGGAGATTT+AGG - chr4.3:76155364-76155383 MS.gene066688:CDS 55.0%
GTTCGCCATCCTTGCCCTAT+CGG + chr4.3:76154951-76154970 None:intergenic 55.0%
TGAGACTGTGACTGTGGTGG+TGG + chr4.3:76154881-76154900 None:intergenic 55.0%
!! GAGGATCCACCGGCTCAATT+AGG - chr4.3:76154912-76154931 MS.gene066688:CDS 55.0%
!! GCTCTAAGGAATGTCCCGGT+TGG - chr4.3:76155109-76155128 MS.gene066688:CDS 55.0%
CCTCATGGAGGAGGATCCAC+CGG - chr4.3:76154902-76154921 MS.gene066688:CDS 60.0%
GATTTCGTCGTCGTCGGAGG+AGG - chr4.3:76155355-76155374 MS.gene066688:CDS 60.0%
GCCCTATCGGACATCGATCC+TGG + chr4.3:76154938-76154957 None:intergenic 60.0%
GGCCAGGATCGATGTCCGAT+AGG - chr4.3:76154933-76154952 MS.gene066688:CDS 60.0%
GTTTGGCGGTCAGAGCTAGC+CGG + chr4.3:76155199-76155218 None:intergenic 60.0%
TCCTGGCCTAATTGAGCCGG+TGG + chr4.3:76154921-76154940 None:intergenic 60.0%
TGCAGCCAACTGGCAGCAAC+AGG - chr4.3:76154538-76154557 MS.gene066688:CDS 60.0%
TTCAGTGCTGCCTCCGGCAT+AGG + chr4.3:76154977-76154996 None:intergenic 60.0%
!! AGGAATGTCCCGGTTGGTGG+AGG - chr4.3:76155115-76155134 MS.gene066688:CDS 60.0%
!! CGGCGCTCTAAGGAATGTCC+CGG - chr4.3:76155105-76155124 MS.gene066688:CDS 60.0%
!! CTAGCTCTGACCGCCAAACG+GGG - chr4.3:76155200-76155219 MS.gene066688:CDS 60.0%
!! GACTGTGACTGTGGTGGTGG+TGG + chr4.3:76154878-76154897 None:intergenic 60.0%
!! GCTAGCTCTGACCGCCAAAC+GGG - chr4.3:76155199-76155218 MS.gene066688:CDS 60.0%
!! GGCTAGCTCTGACCGCCAAA+CGG - chr4.3:76155198-76155217 MS.gene066688:CDS 60.0%
!! TCCACCGGCTCAATTAGGCC+AGG - chr4.3:76154917-76154936 MS.gene066688:CDS 60.0%
!! TGTGACTGTGGTGGTGGTGG+TGG + chr4.3:76154875-76154894 None:intergenic 60.0%
CCGGTGGATCCTCCTCCATG+AGG + chr4.3:76154905-76154924 None:intergenic 65.0%
CTCCGGAAGCCTCCACCAAC+CGG + chr4.3:76155127-76155146 None:intergenic 65.0%
GGACAAGAGGCGGCGCTCTA+AGG - chr4.3:76155095-76155114 MS.gene066688:CDS 65.0%
GGCGAACATGCCTATGCCGG+AGG - chr4.3:76154964-76154983 MS.gene066688:CDS 65.0%
TCCGGAAGCCTCCACCAACC+GGG + chr4.3:76155126-76155145 None:intergenic 65.0%
!! GACTGTGGTGGTGGTGGTGG+TGG + chr4.3:76154872-76154891 None:intergenic 65.0%
!! TCCCGGTTGGTGGAGGCTTC+CGG - chr4.3:76155122-76155141 MS.gene066688:CDS 65.0%
GAGACGGAGCCGCCGTTTCC+TGG + chr4.3:76154830-76154849 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 76154503 76155849 76154503 ID=MS.gene066688
chr4.3 mRNA 76154503 76155849 76154503 ID=MS.gene066688.t1;Parent=MS.gene066688
chr4.3 exon 76155793 76155849 76155793 ID=MS.gene066688.t1.exon1;Parent=MS.gene066688.t1
chr4.3 CDS 76155793 76155849 76155793 ID=cds.MS.gene066688.t1;Parent=MS.gene066688.t1
chr4.3 exon 76154503 76155546 76154503 ID=MS.gene066688.t1.exon2;Parent=MS.gene066688.t1
chr4.3 CDS 76154503 76155546 76154503 ID=cds.MS.gene066688.t1;Parent=MS.gene066688.t1
Gene Sequence

>MS.gene066688

ATGGTTTTTACTTCCATACCAGCAGCTTATCTTGATGCAGCCAACTGGCAGCAACAGGTAAATATAATTAGCAAGGTTTCCATTTAATTTGGTTCTTGCTTTTGTTTCTTTGTCCAAAAGGCAAAATAATTCAAAAGATAAAAAGGAAAAACAATATTCTTGTTACTTTAATTTTATTTATACCTCTTCTTGAATATACCAAATATTTGATTTCTTTCTTATTCCCTTTCTTCCCTTATTCTCAATTCATATACTCTTTTTCTAATTTTTCTTCCTTTTTGTCTATTTATGTTGTTATTTCAGCAACAGAATCATCATCAGCAACCAGGAAACGGCGGCTCCGTCTCTCAACAACTGCTTCAGACACCACCACCACCACCACCACAGTCACAGTCTCAACCTCATGGAGGAGGATCCACCGGCTCAATTAGGCCAGGATCGATGTCCGATAGGGCAAGGATGGCGAACATGCCTATGCCGGAGGCAGCACTGAAATGTCCACGTTGTGAATCAACCAACACAAAATTTTGTTACTTCAACAACTACAGTCTCTCTCAGCCCAGACACTTCTGCAAGACTTGCAGAAGGTACTGGACAAGAGGCGGCGCTCTAAGGAATGTCCCGGTTGGTGGAGGCTTCCGGAGAAACAAGAGATCAAGCAAAAGTAGCAACAATGGAAACTCAACAAAGTCTCCGGCTAGCTCTGACCGCCAAACGGGGAGTGCTAGTTCAACAAACTCAATCACCTCCAATAACAATATCCACTCTTCCGCCGATATATTAGGCCTTACACCTCAAATGTCATCATTGAGGTTCATGACACCTTTACATCATAACTTCAGTAACCCTAATGATTTCGTCGTCGTCGGAGGAGGAGATTTAGGGTTAAACTATGGTTTTAGTTACATGGGAGGAGTAGGTGATTTGGGAAGTGCTTTAGGAGGTAATTCAATTTTATCTTCAAGTGGTTTGGAGCAATGGAGGATGCCAATGTCAATGACTCATCAACAGCATCAATTTCCTTTTTTGGCTAATTTGGAGGGTTCAAATAGTAATTTGTACCCTTTTGAAGGAAATGTTCAAAATCATGAAATGATAACTAGTGGTGGATATGTGAGGCCAAAAGTGATTTCAACTTCTGGAATTATGACTCAACTTGCTTCTGTGAAAATGGAAGATAGTATCAATAGGGGCTTTTTGGGAATTAGCACAGGAAACAATAACAGTAATCAAGGAAGTGAACAATTTTGGAATAGTGCTGTTGTTACTGGTAATTCTGCAGCTAATTGGACTGATCATGTTTCTGGTTTTAGCTCTTCTTCTAATACTACTAGTAACCAGATGTAG
Protein sequence

>MS.gene066688.t1

MVFTSIPAAYLDAANWQQQQQNHHQQPGNGGSVSQQLLQTPPPPPPQSQSQPHGGGSTGSIRPGSMSDRARMANMPMPEAALKCPRCESTNTKFCYFNNYSLSQPRHFCKTCRRYWTRGGALRNVPVGGGFRRNKRSSKSSNNGNSTKSPASSDRQTGSASSTNSITSNNNIHSSADILGLTPQMSSLRFMTPLHHNFSNPNDFVVVGGGDLGLNYGFSYMGGVGDLGSALGGNSILSSSGLEQWRMPMSMTHQQHQFPFLANLEGSNSNLYPFEGNVQNHEMITSGGYVRPKVISTSGIMTQLASVKMEDSINRGFLGISTGNNNSNQGSEQFWNSAVVTGNSAANWTDHVSGFSSSSNTTSNQM