Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066689.t1 | AES63738.2 | 86 | 121 | 17 | 0 | 1 | 121 | 1 | 121 | 5.70E-55 | 223.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066689.t1 | G7INX6 | 86.0 | 121 | 17 | 0 | 1 | 121 | 1 | 121 | 4.1e-55 | 223.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene066689.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066689.t1 | MTR_2g012700 | 85.950 | 121 | 17 | 0 | 1 | 121 | 1 | 121 | 2.47e-73 | 214 |
| MS.gene066689.t1 | MTR_2g062330 | 80.672 | 119 | 22 | 1 | 3 | 121 | 23 | 140 | 1.01e-65 | 196 |
| MS.gene066689.t1 | MTR_7g117585 | 38.000 | 100 | 60 | 1 | 18 | 115 | 42 | 141 | 1.02e-20 | 81.6 |
| MS.gene066689.t1 | MTR_5g071410 | 38.000 | 100 | 60 | 1 | 18 | 115 | 165 | 264 | 1.39e-19 | 81.6 |
| MS.gene066689.t1 | MTR_7g117590 | 35.338 | 133 | 67 | 5 | 7 | 122 | 44 | 174 | 3.18e-19 | 78.6 |
| MS.gene066689.t1 | MTR_0010s0310 | 43.956 | 91 | 49 | 1 | 22 | 110 | 150 | 240 | 1.53e-18 | 78.6 |
| MS.gene066689.t1 | MTR_3g008190 | 28.866 | 97 | 69 | 0 | 24 | 120 | 6 | 102 | 3.23e-17 | 71.6 |
| MS.gene066689.t1 | MTR_5g078270 | 36.264 | 91 | 56 | 1 | 22 | 110 | 208 | 298 | 3.56e-13 | 64.7 |
| MS.gene066689.t1 | MTR_0010s0320 | 38.462 | 91 | 52 | 2 | 24 | 110 | 192 | 282 | 1.47e-12 | 62.8 |
| MS.gene066689.t1 | MTR_0010s0290 | 38.462 | 91 | 52 | 2 | 24 | 110 | 223 | 313 | 2.80e-12 | 62.0 |
| MS.gene066689.t1 | MTR_6g477950 | 34.568 | 81 | 53 | 0 | 39 | 119 | 64 | 144 | 7.27e-12 | 60.8 |
| MS.gene066689.t1 | MTR_8g069780 | 24.719 | 89 | 67 | 0 | 31 | 119 | 67 | 155 | 5.09e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATGTTACTGCTTCTATTT+TGG | 0.123735 | 4.3:+76164878 | MS.gene066689:CDS |
| GAAGTCAATGATCCTAAATT+AGG | 0.174988 | 4.3:+76164819 | MS.gene066689:CDS |
| AAAATGTCGATGTTCATTAT+TGG | 0.262235 | 4.3:+76164850 | MS.gene066689:CDS |
| TGTATGCTACCGTCGTTAAC+TGG | 0.266398 | 4.3:+76165237 | MS.gene066689:CDS |
| TACCAGTTTCTTCGTCAAAA+AGG | 0.277417 | 4.3:-76165070 | None:intergenic |
| TTTGGATGGAAACAATGTTT+TGG | 0.356448 | 4.3:+76164896 | MS.gene066689:CDS |
| CCGTCAATAATCCTCCTAAT+AGG | 0.365364 | 4.3:+76165213 | MS.gene066689:CDS |
| GTATGACTTTGTTCTTGAAA+AGG | 0.398223 | 4.3:+76165160 | MS.gene066689:CDS |
| AAGTCAATGATCCTAAATTA+GGG | 0.407284 | 4.3:+76164820 | MS.gene066689:CDS |
| TATGACTTTGTTCTTGAAAA+GGG | 0.438949 | 4.3:+76165161 | MS.gene066689:CDS |
| ACGGTAGCATACATCCTATT+AGG | 0.440754 | 4.3:-76165227 | None:intergenic |
| GTATGCTACCGTCGTTAACT+GGG | 0.475193 | 4.3:+76165238 | MS.gene066689:CDS |
| CTTGAAAAGGGCCTAAAAGT+TGG | 0.503487 | 4.3:+76165173 | MS.gene066689:CDS |
| GCATGCATCAGAAGTACATA+GGG | 0.543465 | 4.3:+76165129 | MS.gene066689:CDS |
| AGCATGCATCAGAAGTACAT+AGG | 0.566294 | 4.3:+76165128 | MS.gene066689:CDS |
| CCTATTAGGAGGATTATTGA+CGG | 0.567175 | 4.3:-76165213 | None:intergenic |
| AGAAGTACATAGGGAGAGGA+TGG | 0.574105 | 4.3:+76165138 | MS.gene066689:CDS |
| CATCAGAAGTACATAGGGAG+AGG | 0.578101 | 4.3:+76165134 | MS.gene066689:CDS |
| GTAGCATACATCCTATTAGG+AGG | 0.633251 | 4.3:-76165224 | None:intergenic |
| ACATTCTCAGCAACACCTCG+AGG | 0.684927 | 4.3:-76165020 | None:intergenic |
| TATGCTACCGTCGTTAACTG+GGG | 0.696887 | 4.3:+76165239 | MS.gene066689:CDS |
| AAATTATCCCCAGTTAACGA+CGG | 0.712817 | 4.3:-76165246 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATGTCGATGTTCATTAT+TGG | + | chr4.3:76164850-76164869 | MS.gene066689:CDS | 25.0% |
| ! | AAGTCAATGATCCTAAATTA+GGG | + | chr4.3:76164820-76164839 | MS.gene066689:CDS | 25.0% |
| ! | TATGACTTTGTTCTTGAAAA+GGG | + | chr4.3:76165161-76165180 | MS.gene066689:CDS | 25.0% |
| !! | AAATAATGTCACCAACTTTT+AGG | - | chr4.3:76165187-76165206 | None:intergenic | 25.0% |
| !! | TAATGTTACTGCTTCTATTT+TGG | + | chr4.3:76164878-76164897 | MS.gene066689:CDS | 25.0% |
| !! | TTATGTTGTTAAGTTGTTGT+TGG | + | chr4.3:76164950-76164969 | MS.gene066689:intron | 25.0% |
| !! | TTTTGAACATTCCCTAATTT+AGG | - | chr4.3:76164834-76164853 | None:intergenic | 25.0% |
| CAACAAAAATGCTTCGTAAA+TGG | - | chr4.3:76164928-76164947 | None:intergenic | 30.0% | |
| GAAGTCAATGATCCTAAATT+AGG | + | chr4.3:76164819-76164838 | MS.gene066689:CDS | 30.0% | |
| GTATGACTTTGTTCTTGAAA+AGG | + | chr4.3:76165160-76165179 | MS.gene066689:CDS | 30.0% | |
| ! | TTTGGATGGAAACAATGTTT+TGG | + | chr4.3:76164896-76164915 | MS.gene066689:CDS | 30.0% |
| CCTATTAGGAGGATTATTGA+CGG | - | chr4.3:76165216-76165235 | None:intergenic | 35.0% | |
| TACCAGTTTCTTCGTCAAAA+AGG | - | chr4.3:76165073-76165092 | None:intergenic | 35.0% | |
| ! | TACTTCTGATGCATGCTTTT+TGG | - | chr4.3:76165125-76165144 | None:intergenic | 35.0% |
| !! | GTTACTGCTTCTATTTTGGA+TGG | + | chr4.3:76164882-76164901 | MS.gene066689:CDS | 35.0% |
| ACGGTAGCATACATCCTATT+AGG | - | chr4.3:76165230-76165249 | None:intergenic | 40.0% | |
| CCGTCAATAATCCTCCTAAT+AGG | + | chr4.3:76165213-76165232 | MS.gene066689:CDS | 40.0% | |
| CTTGAAAAGGGCCTAAAAGT+TGG | + | chr4.3:76165173-76165192 | MS.gene066689:CDS | 40.0% | |
| GTAGCATACATCCTATTAGG+AGG | - | chr4.3:76165227-76165246 | None:intergenic | 40.0% | |
| ! | AGCATGCATCAGAAGTACAT+AGG | + | chr4.3:76165128-76165147 | MS.gene066689:CDS | 40.0% |
| ! | CTATTGTAGCATTTTCCTCG+AGG | + | chr4.3:76165005-76165024 | MS.gene066689:intron | 40.0% |
| ! | CTCCTTTTTGACGAAGAAAC+TGG | + | chr4.3:76165068-76165087 | MS.gene066689:CDS | 40.0% |
| ! | GCATGCATCAGAAGTACATA+GGG | + | chr4.3:76165129-76165148 | MS.gene066689:CDS | 40.0% |
| GTATGCTACCGTCGTTAACT+GGG | + | chr4.3:76165238-76165257 | MS.gene066689:CDS | 45.0% | |
| TATGCTACCGTCGTTAACTG+GGG | + | chr4.3:76165239-76165258 | MS.gene066689:CDS | 45.0% | |
| TGTATGCTACCGTCGTTAAC+TGG | + | chr4.3:76165237-76165256 | MS.gene066689:CDS | 45.0% | |
| ! | AGAAGTACATAGGGAGAGGA+TGG | + | chr4.3:76165138-76165157 | MS.gene066689:CDS | 45.0% |
| ! | CATCAGAAGTACATAGGGAG+AGG | + | chr4.3:76165134-76165153 | MS.gene066689:CDS | 45.0% |
| ACATTCTCAGCAACACCTCG+AGG | - | chr4.3:76165023-76165042 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 76164801 | 76165265 | 76164801 | ID=MS.gene066689 |
| chr4.3 | mRNA | 76164801 | 76165265 | 76164801 | ID=MS.gene066689.t1;Parent=MS.gene066689 |
| chr4.3 | exon | 76164801 | 76164917 | 76164801 | ID=MS.gene066689.t1.exon1;Parent=MS.gene066689.t1 |
| chr4.3 | CDS | 76164801 | 76164917 | 76164801 | ID=cds.MS.gene066689.t1;Parent=MS.gene066689.t1 |
| chr4.3 | exon | 76165014 | 76165265 | 76165014 | ID=MS.gene066689.t1.exon2;Parent=MS.gene066689.t1 |
| chr4.3 | CDS | 76165014 | 76165265 | 76165014 | ID=cds.MS.gene066689.t1;Parent=MS.gene066689.t1 |
| Gene Sequence |
| Protein sequence |
