AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066710.t1 MTR_4g021855 84.385 301 46 1 1 300 1 301 0.0 526
MS.gene066710.t1 MTR_4g021790 77.667 300 41 1 1 300 1 274 7.16e-171 474
MS.gene066710.t1 MTR_4g021845 83.871 217 35 0 1 217 1 217 4.10e-135 382
MS.gene066710.t1 MTR_4g021760 69.963 273 76 2 1 267 1 273 1.53e-132 379
MS.gene066710.t1 MTR_5g055260 61.364 264 75 4 1 264 1 237 2.34e-98 290
MS.gene066710.t1 MTR_4g023980 76.119 134 32 0 58 191 38 171 4.78e-73 223
MS.gene066710.t1 MTR_1g032570 45.600 250 99 4 10 225 11 257 7.31e-66 217
MS.gene066710.t1 MTR_6g045327 45.417 240 120 5 10 247 14 244 1.05e-57 190
MS.gene066710.t1 MTR_2g034960 38.351 279 142 4 10 261 29 304 2.03e-56 192
MS.gene066710.t1 MTR_4g121020 42.213 244 111 3 10 225 31 272 1.20e-55 191
MS.gene066710.t1 MTR_4g121020 42.213 244 111 3 10 225 31 272 1.36e-55 191
MS.gene066710.t1 MTR_3g086100 41.803 244 114 5 10 225 13 256 3.80e-54 185
MS.gene066710.t1 MTR_8g079940 41.152 243 117 4 9 225 24 266 7.15e-54 184
MS.gene066710.t1 MTR_8g105600 41.803 244 122 2 10 233 9 252 1.85e-53 183
MS.gene066710.t1 MTR_4g131600 39.076 238 122 4 10 224 20 257 1.66e-47 167
MS.gene066710.t1 MTR_1g013170 38.462 247 124 3 7 225 2 248 2.80e-46 162
MS.gene066710.t1 MTR_3g106220 38.710 248 120 4 10 225 8 255 4.84e-46 164
MS.gene066710.t1 MTR_7g117705 36.179 246 129 4 7 224 2 247 5.47e-44 156
MS.gene066710.t1 MTR_1g013180 37.200 250 129 3 7 228 153 402 6.98e-43 156
MS.gene066710.t1 MTR_4g131570 35.200 250 134 4 10 231 17 266 1.45e-42 154
MS.gene066710.t1 MTR_1g032570 42.932 191 73 3 68 225 1 188 1.75e-42 154
MS.gene066710.t1 MTR_4g131580 35.628 247 134 4 10 231 17 263 2.89e-42 154
MS.gene066710.t1 MTR_2g450070 34.252 254 130 4 10 226 18 271 8.02e-42 153
MS.gene066710.t1 MTR_2g450070 34.252 254 130 4 10 226 18 271 9.40e-42 153
MS.gene066710.t1 MTR_8g093040 40.171 234 119 6 1 229 1 218 2.06e-41 144
MS.gene066710.t1 MTR_6g016850 42.945 163 90 2 10 169 9 171 6.42e-40 137
MS.gene066710.t1 MTR_3g102600 36.406 217 102 2 10 225 8 189 1.13e-39 139
MS.gene066710.t1 MTR_3g086100 41.622 185 81 4 68 225 1 185 5.56e-37 137
MS.gene066710.t1 MTR_3g102590 36.449 214 109 4 10 221 8 196 7.46e-37 132
MS.gene066710.t1 MTR_3g102600 34.597 211 102 2 10 219 8 183 1.50e-35 127
MS.gene066710.t1 MTR_3g102590 35.714 210 108 4 10 217 8 192 5.37e-34 123
MS.gene066710.t1 MTR_7g026400 37.321 209 98 5 45 229 19 218 1.37e-32 127
MS.gene066710.t1 MTR_3g102600 36.047 172 75 1 54 225 4 140 2.37e-32 118
MS.gene066710.t1 MTR_0450s0040 31.330 233 145 3 12 233 6 234 3.97e-30 116
MS.gene066710.t1 MTR_4g121020 41.212 165 68 2 88 225 6 168 9.77e-30 118
MS.gene066710.t1 MTR_3g102600 33.735 166 75 1 54 219 4 134 2.87e-28 106
MS.gene066710.t1 MTR_5g041350 47.126 87 42 3 168 251 15 100 3.92e-17 80.9
MS.gene066710.t1 MTR_6g032990 63.636 55 19 1 168 221 42 96 1.30e-16 79.7
MS.gene066710.t1 MTR_1g013160 31.081 148 75 2 71 191 1 148 3.54e-16 74.7
MS.gene066710.t1 MTR_2g086450 60.000 55 21 1 168 221 45 99 1.57e-15 76.6
MS.gene066710.t1 MTR_8g032710 25.660 265 149 8 12 229 19 282 3.93e-15 75.9
MS.gene066710.t1 MTR_7g098250 46.835 79 41 1 159 236 253 331 3.99e-15 75.9
MS.gene066710.t1 MTR_4g064730 43.878 98 49 4 130 225 95 188 8.76e-15 73.9
MS.gene066710.t1 MTR_7g115530 52.055 73 32 2 168 237 218 290 1.25e-14 73.2
MS.gene066710.t1 MTR_2g016220 48.649 74 32 2 153 220 69 142 1.63e-14 73.6
MS.gene066710.t1 MTR_2g016220 48.649 74 32 2 153 220 69 142 1.83e-14 73.6
MS.gene066710.t1 MTR_7g115530 52.055 73 32 2 168 237 218 290 1.95e-14 72.8
MS.gene066710.t1 MTR_2g090675 41.573 89 51 1 137 224 129 217 2.46e-14 73.2
MS.gene066710.t1 MTR_7g115530 52.055 73 32 2 168 237 218 290 3.04e-14 73.2
MS.gene066710.t1 MTR_7g089010 55.932 59 26 0 167 225 90 148 3.39e-14 72.0
MS.gene066710.t1 MTR_4g081710 56.364 55 23 1 168 221 17 71 5.18e-14 71.6
MS.gene066710.t1 MTR_1g080330 48.684 76 36 2 164 238 171 244 8.22e-14 70.9
MS.gene066710.t1 MTR_7g115530 53.731 67 28 2 168 233 218 282 1.12e-13 70.9
MS.gene066710.t1 MTR_1g080330 47.436 78 38 2 164 240 171 246 1.22e-13 70.9
MS.gene066710.t1 MTR_7g088070 43.564 101 53 3 168 265 50 149 1.22e-13 70.1
MS.gene066710.t1 MTR_1g093080 47.500 80 34 2 149 227 199 271 2.34e-13 70.1
MS.gene066710.t1 MTR_7g088070 47.368 76 37 2 168 240 50 125 2.36e-13 69.3
MS.gene066710.t1 MTR_2g016220 42.857 84 41 3 153 229 69 152 2.47e-13 70.1
MS.gene066710.t1 MTR_4g078810 55.172 58 26 0 167 224 157 214 3.05e-13 70.1
MS.gene066710.t1 MTR_4g098870 55.357 56 25 0 167 222 329 384 6.87e-13 69.3
MS.gene066710.t1 MTR_4g098870 55.357 56 25 0 167 222 309 364 6.99e-13 69.3
MS.gene066710.t1 MTR_0223s0040 53.571 56 25 1 166 220 16 71 9.76e-13 67.4
MS.gene066710.t1 MTR_4g098870 55.357 56 25 0 167 222 329 384 9.78e-13 68.9
MS.gene066710.t1 MTR_4g098870 55.357 56 25 0 167 222 348 403 1.05e-12 68.6
MS.gene066710.t1 MTR_4g098870 55.357 56 25 0 167 222 309 364 1.05e-12 68.6
MS.gene066710.t1 MTR_4g086835 51.667 60 29 0 166 225 272 331 1.14e-12 68.2
MS.gene066710.t1 MTR_5g014040 43.182 88 49 1 157 244 300 386 1.26e-12 68.6
MS.gene066710.t1 MTR_7g069660 51.786 56 26 1 166 220 69 124 1.29e-12 67.8
MS.gene066710.t1 MTR_2g027860 53.425 73 30 3 168 236 49 121 1.35e-12 67.8
MS.gene066710.t1 MTR_4g113140 46.753 77 39 2 158 233 226 301 1.69e-12 67.8
MS.gene066710.t1 MTR_1g053835 44.595 74 38 2 166 238 256 327 1.91e-12 67.8
MS.gene066710.t1 MTR_7g093030 47.143 70 34 2 166 234 210 277 1.94e-12 66.6
MS.gene066710.t1 MTR_3g450310 53.571 56 25 1 166 220 17 72 2.48e-12 65.5
MS.gene066710.t1 MTR_7g093030 53.571 56 25 1 166 220 210 265 3.44e-12 66.6
MS.gene066710.t1 MTR_7g115530 59.615 52 20 1 168 218 218 269 6.85e-12 65.1
MS.gene066710.t1 MTR_1g112370 35.294 102 52 4 154 250 6 98 8.87e-12 65.1
MS.gene066710.t1 MTR_2g023580 53.571 56 25 1 166 220 53 108 9.33e-12 64.3
MS.gene066710.t1 MTR_8g086410 50.000 56 27 1 166 220 107 162 1.08e-11 65.5
MS.gene066710.t1 MTR_8g086410 50.000 56 27 1 166 220 109 164 1.08e-11 65.5
MS.gene066710.t1 MTR_6g444980 56.364 55 23 1 168 221 35 89 1.38e-11 64.7
MS.gene066710.t1 MTR_1g090670 38.261 115 49 5 168 281 44 137 1.51e-11 64.3
MS.gene066710.t1 MTR_6g444980 54.237 59 26 1 164 221 31 89 1.64e-11 64.3
MS.gene066710.t1 MTR_5g017980 51.667 60 29 0 166 225 191 250 1.66e-11 64.3
MS.gene066710.t1 MTR_1g053800 53.968 63 28 1 162 223 171 233 1.67e-11 64.3
MS.gene066710.t1 MTR_5g017980 51.667 60 29 0 166 225 190 249 1.71e-11 64.3
MS.gene066710.t1 MTR_2g084230 43.243 74 38 2 148 220 80 150 1.99e-11 63.9
MS.gene066710.t1 MTR_2g027800 36.207 116 55 5 168 282 39 136 2.01e-11 63.9
MS.gene066710.t1 MTR_5g054300 33.333 114 71 2 114 226 148 257 2.18e-11 64.3
MS.gene066710.t1 MTR_2g084230 43.243 74 38 2 148 220 80 150 2.52e-11 63.9
MS.gene066710.t1 MTR_5g027440 46.377 69 34 2 164 229 21 89 3.39e-11 63.2
MS.gene066710.t1 MTR_4g086835 50.820 61 29 1 166 225 272 332 3.96e-11 63.5
MS.gene066710.t1 MTR_1g053830 49.057 53 26 1 170 221 20 72 4.36e-11 58.9
MS.gene066710.t1 MTR_2g027800 51.667 60 28 1 168 226 39 98 6.58e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066710.t1 AT2G01760 39.924 263 119 5 7 232 4 264 7.41e-57 188
MS.gene066710.t1 AT3G16857 37.171 304 134 8 10 261 33 331 7.61e-52 181
MS.gene066710.t1 AT3G16857 37.171 304 134 8 10 261 33 331 1.03e-51 181
MS.gene066710.t1 AT2G01760 36.082 291 119 6 7 232 4 292 6.46e-51 173
MS.gene066710.t1 AT1G67710 39.754 244 118 4 11 225 8 251 5.00e-48 168
MS.gene066710.t1 AT4G31920 38.889 234 129 5 10 230 13 245 4.22e-44 158
MS.gene066710.t1 AT2G25180 38.430 242 121 6 10 225 13 252 7.26e-43 155
MS.gene066710.t1 AT3G16857 35.849 265 113 8 49 261 2 261 1.91e-39 146
MS.gene066710.t1 AT5G58080 35.915 284 141 9 10 266 14 283 5.33e-38 142
MS.gene066710.t1 AT2G01760 37.811 201 87 4 68 232 1 199 6.45e-37 134
MS.gene066710.t1 AT3G62670 33.333 222 125 6 28 226 53 274 2.88e-33 126
MS.gene066710.t1 AT1G49190 29.213 267 152 7 10 243 30 292 2.54e-32 124
MS.gene066710.t1 AT1G67710 39.785 186 84 3 68 225 1 186 2.03e-31 122
MS.gene066710.t1 AT5G49240 32.627 236 132 5 9 217 37 272 1.52e-30 116
MS.gene066710.t1 AT5G07210 31.399 293 141 9 14 252 16 302 1.16e-28 115
MS.gene066710.t1 AT5G07210 30.565 301 141 10 14 252 16 310 3.37e-26 108
MS.gene066710.t1 AT1G67710 38.554 166 74 3 88 225 12 177 6.16e-23 98.6
MS.gene066710.t1 AT2G27070 28.716 296 148 10 14 252 16 305 2.20e-22 97.4
MS.gene066710.t1 AT1G68210 24.825 286 158 6 8 237 7 291 2.53e-20 91.7
MS.gene066710.t1 AT1G49190 34.677 124 76 2 10 130 30 151 7.81e-19 87.0
MS.gene066710.t1 AT3G04030 61.818 55 20 1 168 221 45 99 3.17e-16 78.6
MS.gene066710.t1 AT3G04030 61.818 55 20 1 168 221 45 99 3.30e-16 78.6
MS.gene066710.t1 AT5G29000 38.938 113 61 3 134 245 161 266 3.53e-16 78.6
MS.gene066710.t1 AT5G29000 38.938 113 61 3 134 245 161 266 3.53e-16 78.6
MS.gene066710.t1 AT5G29000 38.938 113 61 3 134 245 204 309 4.02e-16 78.6
MS.gene066710.t1 AT2G01060 49.398 83 39 2 157 236 4 86 4.43e-16 77.4
MS.gene066710.t1 AT5G18240 61.818 55 20 1 168 221 45 99 6.84e-16 77.8
MS.gene066710.t1 AT5G18240 61.818 55 20 1 168 221 45 99 6.84e-16 77.8
MS.gene066710.t1 AT5G29000 40.777 103 53 3 134 235 204 299 7.32e-16 77.0
MS.gene066710.t1 AT5G18240 61.818 55 20 1 168 221 45 99 7.58e-16 77.8
MS.gene066710.t1 AT5G18240 61.818 55 20 1 168 221 45 99 7.94e-16 77.8
MS.gene066710.t1 AT5G18240 61.818 55 20 1 168 221 45 99 7.94e-16 77.8
MS.gene066710.t1 AT3G46640 44.706 85 44 1 156 237 129 213 2.91e-15 75.5
MS.gene066710.t1 AT3G46640 44.706 85 44 1 156 237 129 213 3.47e-15 75.1
MS.gene066710.t1 AT3G46640 44.706 85 44 1 156 237 129 213 3.47e-15 75.1
MS.gene066710.t1 AT3G04030 57.407 54 23 0 168 221 45 98 3.76e-15 75.5
MS.gene066710.t1 AT5G59570 40.952 105 56 2 156 257 126 227 3.82e-15 74.7
MS.gene066710.t1 AT5G59570 40.952 105 56 2 156 257 126 227 3.82e-15 74.7
MS.gene066710.t1 AT5G05090 55.932 59 26 0 167 225 80 138 1.04e-14 73.2
MS.gene066710.t1 AT3G10760 55.932 59 26 0 167 225 104 162 1.99e-14 73.2
MS.gene066710.t1 AT2G40970 54.237 59 27 0 167 225 104 162 3.01e-14 71.6
MS.gene066710.t1 AT5G44190 58.621 58 24 0 167 224 146 203 7.22e-14 71.6
MS.gene066710.t1 AT5G44190 58.621 58 24 0 167 224 146 203 8.31e-14 71.2
MS.gene066710.t1 AT3G04450 57.407 54 22 1 168 220 238 291 8.53e-14 71.6
MS.gene066710.t1 AT3G04450 57.407 54 22 1 168 220 198 251 9.13e-14 71.6
MS.gene066710.t1 AT3G04450 57.407 54 22 1 168 220 198 251 9.13e-14 71.6
MS.gene066710.t1 AT3G04450 57.407 54 22 1 168 220 238 291 1.15e-13 70.5
MS.gene066710.t1 AT4G28610 60.000 55 21 1 168 221 225 279 1.41e-13 70.9
MS.gene066710.t1 AT3G24120 38.462 104 58 2 168 270 41 139 3.41e-13 68.9
MS.gene066710.t1 AT3G24120 38.462 104 58 2 168 270 41 139 3.78e-13 68.9
MS.gene066710.t1 AT1G69580 49.231 65 32 1 168 231 34 98 4.18e-13 69.3
MS.gene066710.t1 AT1G69580 49.231 65 32 1 168 231 34 98 4.32e-13 69.3
MS.gene066710.t1 AT2G20570 45.783 83 39 1 148 224 127 209 7.47e-13 68.9
MS.gene066710.t1 AT1G25550 48.000 75 38 1 154 227 195 269 8.05e-13 68.6
MS.gene066710.t1 AT2G40260 40.594 101 53 3 134 233 51 145 8.68e-13 68.6
MS.gene066710.t1 AT1G14600 61.538 52 19 1 170 220 25 76 1.24e-12 67.0
MS.gene066710.t1 AT5G45580 38.739 111 59 3 164 272 19 122 1.40e-12 67.0
MS.gene066710.t1 AT5G45580 38.739 111 59 3 164 272 3 106 1.44e-12 67.0
MS.gene066710.t1 AT5G06800 51.471 68 30 2 168 234 192 257 2.55e-12 67.0
MS.gene066710.t1 AT1G79430 43.617 94 48 2 149 237 15 108 2.74e-12 67.0
MS.gene066710.t1 AT5G06800 58.182 55 22 1 168 221 192 246 3.28e-12 66.2
MS.gene066710.t1 AT3G13040 53.571 56 25 1 167 221 240 295 3.60e-12 67.0
MS.gene066710.t1 AT3G13040 53.571 56 25 1 167 221 240 295 3.60e-12 67.0
MS.gene066710.t1 AT3G13040 53.571 56 25 1 167 221 240 295 3.60e-12 67.0
MS.gene066710.t1 AT4G13640 51.471 68 29 2 168 231 37 104 3.81e-12 65.9
MS.gene066710.t1 AT4G13640 51.471 68 29 2 168 231 37 104 3.93e-12 65.9
MS.gene066710.t1 AT4G04605 47.222 72 36 2 166 235 12 83 4.86e-12 64.7
MS.gene066710.t1 AT5G06800 58.182 55 22 1 168 221 192 246 5.10e-12 66.2
MS.gene066710.t1 AT2G20570 58.182 55 23 0 170 224 171 225 7.07e-12 65.9
MS.gene066710.t1 AT1G68670 46.429 84 43 2 145 227 193 275 7.23e-12 65.5
MS.gene066710.t1 AT2G20400 59.259 54 21 1 168 220 231 284 9.52e-12 65.5
MS.gene066710.t1 AT2G20400 59.259 54 21 1 168 220 231 284 9.72e-12 65.5
MS.gene066710.t1 AT3G12730 52.727 55 25 1 168 221 23 77 1.57e-11 63.5
MS.gene066710.t1 AT4G18020 48.485 66 33 1 159 224 288 352 2.31e-11 64.7
MS.gene066710.t1 AT4G18020 48.485 66 33 1 159 224 288 352 2.31e-11 64.7
MS.gene066710.t1 AT4G18020 48.485 66 33 1 159 224 288 352 2.31e-11 64.7
MS.gene066710.t1 AT4G18020 48.485 66 33 1 159 224 288 352 2.31e-11 64.7
MS.gene066710.t1 AT4G18020 48.485 66 33 1 159 224 288 352 2.31e-11 64.7
MS.gene066710.t1 AT4G18020 48.485 66 33 1 159 224 288 352 2.43e-11 64.3
MS.gene066710.t1 AT4G18020 48.485 66 33 1 159 224 288 352 2.43e-11 64.3
MS.gene066710.t1 AT4G18020 48.485 66 33 1 159 224 288 352 2.43e-11 64.3
MS.gene066710.t1 AT4G18020 48.485 66 33 1 159 224 288 352 2.43e-11 64.3
MS.gene066710.t1 AT4G18020 48.485 66 33 1 159 224 288 352 2.43e-11 64.3
MS.gene066710.t1 AT4G18020 48.485 66 33 1 159 224 288 352 2.43e-11 64.3
MS.gene066710.t1 AT2G03500 50.000 62 30 1 167 227 232 293 2.54e-11 64.3
MS.gene066710.t1 AT2G38300 48.214 56 28 1 166 220 52 107 4.57e-11 63.2
MS.gene066710.t1 AT2G42660 43.750 64 35 1 158 220 40 103 5.50e-11 62.0

Find 64 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATGATTCTCAAAATTA+TGG 0.243357 4.3:+76373493 MS.gene066710:CDS
ACATGTTGCTAGTCATTTAC+AGG 0.286115 4.3:+76373538 MS.gene066710:CDS
AACTGCATCAACGATTTCTA+TGG 0.292867 4.3:+76373346 MS.gene066710:CDS
ATCCTACCTTTACAACCTTA+TGG 0.307805 4.3:+76373905 MS.gene066710:CDS
CTCAAAATTATGGATGTTCC+AGG 0.335551 4.3:+76373503 MS.gene066710:CDS
AATTATGGATGTTCCAGGTT+TGG 0.345656 4.3:+76373508 MS.gene066710:CDS
ATTATGGATGTTCCAGGTTT+GGG 0.349053 4.3:+76373509 MS.gene066710:CDS
CAGTTTGAATGTTATCGTTC+CGG 0.359832 4.3:-76373821 None:intergenic
AGAAATCGTTGATGCAGTTC+TGG 0.372917 4.3:-76373342 None:intergenic
AAAGTGACTAACCTTGATAA+TGG 0.378712 4.3:-76372703 None:intergenic
TACATATTGGACTAAGCCTT+TGG 0.386876 4.3:+76373146 MS.gene066710:CDS
GGTTGAAGAAAATAGAAAAC+GGG 0.411991 4.3:+76373257 MS.gene066710:CDS
ATCAGAAGCTGTGTTACATT+TGG 0.412873 4.3:-76372844 None:intergenic
AATGTGGGGCTTGTACATAT+TGG 0.414028 4.3:+76373133 MS.gene066710:CDS
AATAAAGAATATGAAATTGT+TGG 0.416766 4.3:+76373225 MS.gene066710:CDS
AAACTGCATATTAGGCCATA+AGG 0.423301 4.3:-76373920 None:intergenic
AATGTCGTTTGCAGAAATAT+AGG 0.426550 4.3:+76373667 MS.gene066710:intron
TGTAATCGATTCCATTATCA+AGG 0.450836 4.3:+76372692 MS.gene066710:CDS
TTCTGGCGACCAAGACAAAC+GGG 0.460294 4.3:-76373325 None:intergenic
AATAAATTCTTACTAATGAC+AGG 0.461511 4.3:-76372981 None:intergenic
ATATGAAATTGTTGGAAGCT+TGG 0.461542 4.3:+76373233 MS.gene066710:CDS
TTGAGACCAGCAGGGAATGA+TGG 0.467536 4.3:-76372617 None:intergenic
AAAAGATACACATGCAAAGA+AGG 0.470493 4.3:+76373302 MS.gene066710:CDS
AAGTCATAAGCATCCATGTT+TGG 0.471983 4.3:-76372927 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.473177 4.3:+76373316 MS.gene066710:CDS
TAGCTGAGTGGATTGATTGT+GGG 0.482429 4.3:+76373877 MS.gene066710:CDS
CTAGCTGAGTGGATTGATTG+TGG 0.491435 4.3:+76373876 MS.gene066710:CDS
CACTTTCCTCAACTCTATTG+AGG 0.492388 4.3:+76372664 MS.gene066710:CDS
CAGCTAGAAAACCATCATTC+CGG 0.492887 4.3:-76373860 None:intergenic
GTTCTGGCGACCAAGACAAA+CGG 0.493157 4.3:-76373326 None:intergenic
GCAATAACCTTGAGACCAGC+AGG 0.494358 4.3:-76372626 None:intergenic
TCAAATTCAATCGATCGATC+AGG 0.496009 4.3:+76373797 MS.gene066710:CDS
GATGTTCCAGGTTTGGGTGT+AGG 0.505057 4.3:+76373515 MS.gene066710:CDS
TGGTTGAAGAAAATAGAAAA+CGG 0.506154 4.3:+76373256 MS.gene066710:CDS
TAGGATTCATTTGAAGAGAT+CGG 0.515087 4.3:+76373686 MS.gene066710:CDS
ACTGATCACGACCGGAATGA+TGG 0.518029 4.3:+76373849 MS.gene066710:CDS
CTATGGGCTGTCAATCAACT+AGG 0.518108 4.3:+76373363 MS.gene066710:CDS
ATCATTCCCTGCTGGTCTCA+AGG 0.520371 4.3:+76372619 MS.gene066710:CDS
AGGAAAGAAGCATACTATCA+AGG 0.524721 4.3:-76373386 None:intergenic
TTATGAAGTCTATTCAATGT+GGG 0.525603 4.3:+76373118 MS.gene066710:CDS
ATTAGGCCATAAGGTTGTAA+AGG 0.531488 4.3:-76373911 None:intergenic
GAGTGGATTGATTGTGGGAC+GGG 0.533110 4.3:+76373882 MS.gene066710:CDS
ACATGTCCTACACCCAAACC+TGG 0.534820 4.3:-76373521 None:intergenic
TTGACGTCTTCTTCAACCAA+AGG 0.537631 4.3:-76373162 None:intergenic
GACAACCCATCATTCCCTGC+TGG 0.539365 4.3:+76372611 MS.gene066710:CDS
TGAGACCAGCAGGGAATGAT+GGG 0.539790 4.3:-76372616 None:intergenic
TGGCAACATGTTGTCAGAAA+CGG 0.543020 4.3:+76373192 MS.gene066710:CDS
TTCAATCGATCGATCAGGAC+CGG 0.553886 4.3:+76373802 MS.gene066710:CDS
ATTATGAAGTCTATTCAATG+TGG 0.558796 4.3:+76373117 MS.gene066710:CDS
CTGAGCTGACTGATCACGAC+CGG 0.562799 4.3:+76373841 MS.gene066710:CDS
TGAAATTGTTGGAAGCTTGG+TGG 0.576315 4.3:+76373236 MS.gene066710:CDS
GAAAGTGATGTCATGATCAA+TGG 0.586165 4.3:-76372648 None:intergenic
ACTGCATCAACGATTTCTAT+GGG 0.587946 4.3:+76373347 MS.gene066710:CDS
TCAAGACGAGTGCTTTGACA+AGG 0.588298 4.3:+76373758 MS.gene066710:CDS
GGCCATAAGGTTGTAAAGGT+AGG 0.602949 4.3:-76373907 None:intergenic
TGATGCTCATATGCCAAACA+TGG 0.604750 4.3:+76372914 MS.gene066710:CDS
TATGAAGTCTATTCAATGTG+GGG 0.614025 4.3:+76373119 MS.gene066710:CDS
ACATCTCCTCAATAGAGTTG+AGG 0.625514 4.3:-76372670 None:intergenic
AAGAAGACGTCAAGAGCATG+TGG 0.649026 4.3:+76373172 MS.gene066710:CDS
TGAGTGGATTGATTGTGGGA+CGG 0.659734 4.3:+76373881 MS.gene066710:CDS
CGGCTGATGAAACAAAACGT+GGG 0.694912 4.3:+76373706 MS.gene066710:CDS
CAATAACCTTGAGACCAGCA+GGG 0.699486 4.3:-76372625 None:intergenic
TCGGCTGATGAAACAAAACG+TGG 0.707531 4.3:+76373705 MS.gene066710:CDS
GTTGAAGAAAATAGAAAACG+GGG 0.712341 4.3:+76373258 MS.gene066710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAAGTGAGATAAAAA+TGG - chr4.3:76373460-76373479 None:intergenic 15.0%
!! AATAAAGAATATGAAATTGT+TGG + chr4.3:76373225-76373244 MS.gene066710:CDS 15.0%
!!! ATAATTTTGAGAATCATTTT+GGG - chr4.3:76373494-76373513 None:intergenic 15.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4.3:76372984-76373003 None:intergenic 20.0%
!! CAAAATGATTCTCAAAATTA+TGG + chr4.3:76373493-76373512 MS.gene066710:CDS 20.0%
!!! CATAATTTTGAGAATCATTT+TGG - chr4.3:76373495-76373514 None:intergenic 20.0%
!!! TTATTTCTTTTTTGTAGTGA+TGG + chr4.3:76373071-76373090 MS.gene066710:intron 20.0%
! ACAAAGCAAAGAAATGAATT+AGG - chr4.3:76373409-76373428 None:intergenic 25.0%
! ATTATGAAGTCTATTCAATG+TGG + chr4.3:76373117-76373136 MS.gene066710:CDS 25.0%
! CATTCTTTCCAAAAAATTCA+AGG - chr4.3:76372871-76372890 None:intergenic 25.0%
! TGGTTGAAGAAAATAGAAAA+CGG + chr4.3:76373256-76373275 MS.gene066710:CDS 25.0%
! TTATGAAGTCTATTCAATGT+GGG + chr4.3:76373118-76373137 MS.gene066710:CDS 25.0%
!!! CTTGAATTTTTTGGAAAGAA+TGG + chr4.3:76372869-76372888 MS.gene066710:CDS 25.0%
AAAAGATACACATGCAAAGA+AGG + chr4.3:76373302-76373321 MS.gene066710:CDS 30.0%
AAAGTGACTAACCTTGATAA+TGG - chr4.3:76372706-76372725 None:intergenic 30.0%
ATATGAAATTGTTGGAAGCT+TGG + chr4.3:76373233-76373252 MS.gene066710:CDS 30.0%
GGTTGAAGAAAATAGAAAAC+GGG + chr4.3:76373257-76373276 MS.gene066710:CDS 30.0%
GTTGAAGAAAATAGAAAACG+GGG + chr4.3:76373258-76373277 MS.gene066710:CDS 30.0%
TAGGATTCATTTGAAGAGAT+CGG + chr4.3:76373686-76373705 MS.gene066710:CDS 30.0%
TATGAAGTCTATTCAATGTG+GGG + chr4.3:76373119-76373138 MS.gene066710:CDS 30.0%
! AATGTCGTTTGCAGAAATAT+AGG + chr4.3:76373667-76373686 MS.gene066710:intron 30.0%
! TGTAATCGATTCCATTATCA+AGG + chr4.3:76372692-76372711 MS.gene066710:CDS 30.0%
!! GTCATTTACAGGTTTGTTTT+CGG + chr4.3:76373549-76373568 MS.gene066710:intron 30.0%
!!! TGAGTTTTAGGGTAACATTT+TGG - chr4.3:76372956-76372975 None:intergenic 30.0%
!!! TTCTGATGCCTTGAATTTTT+TGG + chr4.3:76372860-76372879 MS.gene066710:CDS 30.0%
AACTGCATCAACGATTTCTA+TGG + chr4.3:76373346-76373365 MS.gene066710:CDS 35.0%
AAGTCATAAGCATCCATGTT+TGG - chr4.3:76372930-76372949 None:intergenic 35.0%
AATTATGGATGTTCCAGGTT+TGG + chr4.3:76373508-76373527 MS.gene066710:CDS 35.0%
ACATGTTGCTAGTCATTTAC+AGG + chr4.3:76373538-76373557 MS.gene066710:CDS 35.0%
ACTGCATCAACGATTTCTAT+GGG + chr4.3:76373347-76373366 MS.gene066710:CDS 35.0%
ATCAGAAGCTGTGTTACATT+TGG - chr4.3:76372847-76372866 None:intergenic 35.0%
ATCCTACCTTTACAACCTTA+TGG + chr4.3:76373905-76373924 MS.gene066710:CDS 35.0%
ATTATGGATGTTCCAGGTTT+GGG + chr4.3:76373509-76373528 MS.gene066710:CDS 35.0%
CAGTTTGAATGTTATCGTTC+CGG - chr4.3:76373824-76373843 None:intergenic 35.0%
CTCAAAATTATGGATGTTCC+AGG + chr4.3:76373503-76373522 MS.gene066710:CDS 35.0%
GAAAGTGATGTCATGATCAA+TGG - chr4.3:76372651-76372670 None:intergenic 35.0%
GTGGGAAGAAAAAATCAAAG+TGG + chr4.3:76373724-76373743 MS.gene066710:CDS 35.0%
TACATATTGGACTAAGCCTT+TGG + chr4.3:76373146-76373165 MS.gene066710:CDS 35.0%
TCAAATTCAATCGATCGATC+AGG + chr4.3:76373797-76373816 MS.gene066710:CDS 35.0%
! AGGAAAGAAGCATACTATCA+AGG - chr4.3:76373389-76373408 None:intergenic 35.0%
! CTTTGCATGTGTATCTTTTG+AGG - chr4.3:76373301-76373320 None:intergenic 35.0%
! GACAGGAATGTTGAGTTTTA+GGG - chr4.3:76372967-76372986 None:intergenic 35.0%
! TGACAGGAATGTTGAGTTTT+AGG - chr4.3:76372968-76372987 None:intergenic 35.0%
!! TTTACAGGTTTGTTTTCGGA+AGG + chr4.3:76373553-76373572 MS.gene066710:intron 35.0%
AATGTGGGGCTTGTACATAT+TGG + chr4.3:76373133-76373152 MS.gene066710:CDS 40.0%
ACATCTCCTCAATAGAGTTG+AGG - chr4.3:76372673-76372692 None:intergenic 40.0%
AGAAATCGTTGATGCAGTTC+TGG - chr4.3:76373345-76373364 None:intergenic 40.0%
CACTTTCCTCAACTCTATTG+AGG + chr4.3:76372664-76372683 MS.gene066710:CDS 40.0%
CAGCTAGAAAACCATCATTC+CGG - chr4.3:76373863-76373882 None:intergenic 40.0%
TGAAATTGTTGGAAGCTTGG+TGG + chr4.3:76373236-76373255 MS.gene066710:CDS 40.0%
TGATGCTCATATGCCAAACA+TGG + chr4.3:76372914-76372933 MS.gene066710:CDS 40.0%
TGGCAACATGTTGTCAGAAA+CGG + chr4.3:76373192-76373211 MS.gene066710:CDS 40.0%
TTGACGTCTTCTTCAACCAA+AGG - chr4.3:76373165-76373184 None:intergenic 40.0%
!! TAGCTGAGTGGATTGATTGT+GGG + chr4.3:76373877-76373896 MS.gene066710:CDS 40.0%
!!! ATGATGGTTTTCTAGCTGAG+TGG + chr4.3:76373865-76373884 MS.gene066710:CDS 40.0%
CAATAACCTTGAGACCAGCA+GGG - chr4.3:76372628-76372647 None:intergenic 45.0%
CGGCTGATGAAACAAAACGT+GGG + chr4.3:76373706-76373725 MS.gene066710:CDS 45.0%
GGCCATAAGGTTGTAAAGGT+AGG - chr4.3:76373910-76373929 None:intergenic 45.0%
TCAAGACGAGTGCTTTGACA+AGG + chr4.3:76373758-76373777 MS.gene066710:CDS 45.0%
TCGGCTGATGAAACAAAACG+TGG + chr4.3:76373705-76373724 MS.gene066710:CDS 45.0%
TTCAATCGATCGATCAGGAC+CGG + chr4.3:76373802-76373821 MS.gene066710:CDS 45.0%
! AAGAAGACGTCAAGAGCATG+TGG + chr4.3:76373172-76373191 MS.gene066710:CDS 45.0%
! CTATGGGCTGTCAATCAACT+AGG + chr4.3:76373363-76373382 MS.gene066710:CDS 45.0%
!! CTAGCTGAGTGGATTGATTG+TGG + chr4.3:76373876-76373895 MS.gene066710:CDS 45.0%
!! TGAGTGGATTGATTGTGGGA+CGG + chr4.3:76373881-76373900 MS.gene066710:CDS 45.0%
ACATGTCCTACACCCAAACC+TGG - chr4.3:76373524-76373543 None:intergenic 50.0%
ACTGATCACGACCGGAATGA+TGG + chr4.3:76373849-76373868 MS.gene066710:CDS 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4.3:76373316-76373335 MS.gene066710:CDS 50.0%
GATGTTCCAGGTTTGGGTGT+AGG + chr4.3:76373515-76373534 MS.gene066710:CDS 50.0%
GCAATAACCTTGAGACCAGC+AGG - chr4.3:76372629-76372648 None:intergenic 50.0%
GTTCTGGCGACCAAGACAAA+CGG - chr4.3:76373329-76373348 None:intergenic 50.0%
TGAGACCAGCAGGGAATGAT+GGG - chr4.3:76372619-76372638 None:intergenic 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4.3:76373328-76373347 None:intergenic 50.0%
TTGAGACCAGCAGGGAATGA+TGG - chr4.3:76372620-76372639 None:intergenic 50.0%
! ATCATTCCCTGCTGGTCTCA+AGG + chr4.3:76372619-76372638 MS.gene066710:CDS 50.0%
!! GAGTGGATTGATTGTGGGAC+GGG + chr4.3:76373882-76373901 MS.gene066710:CDS 50.0%
CTGAGCTGACTGATCACGAC+CGG + chr4.3:76373841-76373860 MS.gene066710:CDS 55.0%
GACAACCCATCATTCCCTGC+TGG + chr4.3:76372611-76372630 MS.gene066710:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 76372596 76373932 76372596 ID=MS.gene066710
chr4.3 mRNA 76372596 76373932 76372596 ID=MS.gene066710.t1;Parent=MS.gene066710
chr4.3 exon 76372596 76372713 76372596 ID=MS.gene066710.t1.exon1;Parent=MS.gene066710.t1
chr4.3 CDS 76372596 76372713 76372596 ID=cds.MS.gene066710.t1;Parent=MS.gene066710.t1
chr4.3 exon 76372841 76372990 76372841 ID=MS.gene066710.t1.exon2;Parent=MS.gene066710.t1
chr4.3 CDS 76372841 76372990 76372841 ID=cds.MS.gene066710.t1;Parent=MS.gene066710.t1
chr4.3 exon 76373088 76373393 76373088 ID=MS.gene066710.t1.exon3;Parent=MS.gene066710.t1
chr4.3 CDS 76373088 76373393 76373088 ID=cds.MS.gene066710.t1;Parent=MS.gene066710.t1
chr4.3 exon 76373483 76373559 76373483 ID=MS.gene066710.t1.exon4;Parent=MS.gene066710.t1
chr4.3 CDS 76373483 76373559 76373483 ID=cds.MS.gene066710.t1;Parent=MS.gene066710.t1
chr4.3 exon 76373681 76373932 76373681 ID=MS.gene066710.t1.exon5;Parent=MS.gene066710.t1
chr4.3 CDS 76373681 76373932 76373681 ID=cds.MS.gene066710.t1;Parent=MS.gene066710.t1
Gene Sequence

>MS.gene066710

ATGGCTGTTTTCGTTGACAACCCATCATTCCCTGCTGGTCTCAAGGTTATTGCCATTGATCATGACATCACTTTCCTCAACTCTATTGAGGAGATGTGTAATCGATTCCATTATCAAGGTTAGTCACTTTGTTATTTCACTTTATTCAATTCTGAATTTTCTATCTATGTTCTTACTTTTATGTATTTTCATGTTTGTTTGTTTCTTCATTCTTATGCATATTTTTATATGTTTCTTTATTTCAGTTACCAAATGTAACACAGCTTCTGATGCCTTGAATTTTTTGGAAAGAATGGACTGTTTCGATTTAATGTTGATTGATGCTCATATGCCAAACATGGATGCTTATGACTTTGTCCAAAATGTTACCCTAAAACTCAACATTCCTGTCATTAGTAAGAATTTATTCAACTTTACACAATTCACATGATTCATTCATATATATGTACTTAATTAGTGTGAACTGACATTATTATTATTTCTTTTTTGTAGTGATGGCTGTTGATTTCGCAAAAAGTTCTATTATGAAGTCTATTCAATGTGGGGCTTGTACATATTGGACTAAGCCTTTGGTTGAAGAAGACGTCAAGAGCATGTGGCAACATGTTGTCAGAAACGGTTCGACTGAAAATAAAGAATATGAAATTGTTGGAAGCTTGGTGGTTGAAGAAAATAGAAAACGGGGTAGAGAAGATGATAATGCCTCAAAAGATACACATGCAAAGAAGGCCCGTTTGTCTTGGTCGCCAGAACTGCATCAACGATTTCTATGGGCTGTCAATCAACTAGGCCTTGATAGTATGCTTCTTTCCTAATTCATTTCTTTGCTTTGTTTGTATTATTTAACAACTCAACTTATGTCCATTTTTATCTCACTTATTTTTCAGAAGCAAGACCCAAAATGATTCTCAAAATTATGGATGTTCCAGGTTTGGGTGTAGGACATGTTGCTAGTCATTTACAGGTTTGTTTTCGGAAGGAGTCTAATTAAATTATTAATAATATACTACATGTATTTTGAGTGATTTTAAATTGAAACTTACAGAGTACTATTGTTAAAATTGTGATTGCAATGTCGTTTGCAGAAATATAGGATTCATTTGAAGAGATCGGCTGATGAAACAAAACGTGGGAAGAAAAAATCAAAGTGGAGTGATAGCTTTCAAGACGAGTGCTTTGACAAGGTTCATAGTGATAGCTTTCAAATTCAATCGATCGATCAGGACCGGAACGATAACATTCAAACTGAGCTGACTGATCACGACCGGAATGATGGTTTTCTAGCTGAGTGGATTGATTGTGGGACGGGATCCTACCTTTACAACCTTATGGCCTAA
Protein sequence

>MS.gene066710.t1

MAVFVDNPSFPAGLKVIAIDHDITFLNSIEEMCNRFHYQVTKCNTASDALNFLERMDCFDLMLIDAHMPNMDAYDFVQNVTLKLNIPVIMMAVDFAKSSIMKSIQCGACTYWTKPLVEEDVKSMWQHVVRNGSTENKEYEIVGSLVVEENRKRGREDDNASKDTHAKKARLSWSPELHQRFLWAVNQLGLDKARPKMILKIMDVPGLGVGHVASHLQKYRIHLKRSADETKRGKKKSKWSDSFQDECFDKVHSDSFQIQSIDQDRNDNIQTELTDHDRNDGFLAEWIDCGTGSYLYNLMA