AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066712

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066712.t1 MTR_4g021760 82.484 314 52 2 1 313 1 312 0.0 532
MS.gene066712.t1 MTR_4g021855 79.752 242 47 1 1 242 1 240 4.95e-138 392
MS.gene066712.t1 MTR_4g021845 84.932 219 31 1 1 219 1 217 2.60e-136 385
MS.gene066712.t1 MTR_4g021790 75.207 242 58 1 1 242 1 240 1.94e-127 365
MS.gene066712.t1 MTR_5g055260 59.307 231 65 5 1 231 1 202 4.43e-85 256
MS.gene066712.t1 MTR_4g023980 76.978 139 30 1 55 193 35 171 1.64e-71 219
MS.gene066712.t1 MTR_1g032570 38.082 365 159 9 10 309 11 373 7.27e-69 226
MS.gene066712.t1 MTR_6g045327 46.075 293 139 8 10 296 14 293 3.14e-68 218
MS.gene066712.t1 MTR_3g086100 40.316 253 118 5 10 231 13 263 1.12e-57 195
MS.gene066712.t1 MTR_8g079940 39.695 262 125 6 9 239 24 283 2.12e-55 188
MS.gene066712.t1 MTR_4g121020 38.868 265 133 4 10 248 31 292 1.42e-54 189
MS.gene066712.t1 MTR_4g121020 38.868 265 133 4 10 248 31 292 1.52e-54 188
MS.gene066712.t1 MTR_2g034960 39.245 265 133 4 10 248 29 291 1.74e-54 187
MS.gene066712.t1 MTR_8g105600 39.759 249 126 4 4 231 3 248 4.88e-52 180
MS.gene066712.t1 MTR_1g013170 39.200 250 118 4 7 226 2 247 7.02e-50 172
MS.gene066712.t1 MTR_4g131600 36.992 246 134 4 10 234 20 265 3.71e-49 172
MS.gene066712.t1 MTR_7g117705 36.466 266 141 4 7 244 2 267 1.26e-48 169
MS.gene066712.t1 MTR_8g093040 41.949 236 114 6 1 231 1 218 1.26e-46 159
MS.gene066712.t1 MTR_3g106220 37.652 247 124 3 10 226 8 254 3.09e-46 165
MS.gene066712.t1 MTR_1g013180 36.992 246 129 3 7 226 153 398 1.36e-44 161
MS.gene066712.t1 MTR_1g032570 34.314 306 135 8 68 309 1 304 2.59e-44 159
MS.gene066712.t1 MTR_4g131570 32.800 250 142 4 10 233 17 266 1.04e-41 152
MS.gene066712.t1 MTR_4g131580 34.008 247 140 4 10 233 17 263 1.56e-41 152
MS.gene066712.t1 MTR_2g450070 33.333 255 135 3 10 229 18 272 3.22e-41 152
MS.gene066712.t1 MTR_2g450070 33.333 255 135 3 10 229 18 272 3.87e-41 152
MS.gene066712.t1 MTR_3g102590 38.140 215 104 4 10 222 8 195 1.31e-40 142
MS.gene066712.t1 MTR_3g086100 39.691 194 85 4 68 231 1 192 2.54e-39 144
MS.gene066712.t1 MTR_3g102600 36.564 227 104 4 1 226 1 188 1.26e-38 136
MS.gene066712.t1 MTR_6g016850 42.857 161 86 3 4 161 3 160 1.26e-38 134
MS.gene066712.t1 MTR_3g102590 37.264 212 104 4 10 219 8 192 3.62e-38 134
MS.gene066712.t1 MTR_3g102600 35.135 222 104 4 1 221 1 183 4.06e-36 129
MS.gene066712.t1 MTR_0450s0040 29.918 244 160 4 12 248 6 245 1.67e-31 120
MS.gene066712.t1 MTR_7g026400 33.175 211 120 4 52 248 30 233 1.73e-31 124
MS.gene066712.t1 MTR_3g102600 35.838 173 74 2 54 226 4 139 2.46e-31 116
MS.gene066712.t1 MTR_3g102600 33.929 168 74 2 54 221 4 134 9.14e-29 108
MS.gene066712.t1 MTR_4g121020 36.559 186 90 3 88 248 6 188 1.41e-28 115
MS.gene066712.t1 MTR_8g032710 23.249 357 214 9 12 309 19 374 1.03e-21 95.5
MS.gene066712.t1 MTR_1g013160 34.211 152 67 3 71 193 1 148 4.72e-19 82.8
MS.gene066712.t1 MTR_2g090675 38.710 93 55 1 136 226 125 217 7.25e-14 72.0
MS.gene066712.t1 MTR_5g041350 55.556 54 23 1 170 222 15 68 1.49e-13 70.9
MS.gene066712.t1 MTR_7g098250 40.594 101 55 3 161 256 253 353 4.59e-13 69.7
MS.gene066712.t1 MTR_6g032990 44.872 78 42 1 170 246 42 119 4.66e-13 69.7
MS.gene066712.t1 MTR_4g078810 48.276 58 30 0 169 226 157 214 6.95e-13 68.9
MS.gene066712.t1 MTR_7g088070 40.659 91 51 2 168 257 48 136 1.04e-12 67.8
MS.gene066712.t1 MTR_4g081710 51.852 54 25 1 170 222 17 70 1.90e-12 67.4
MS.gene066712.t1 MTR_2g086450 53.704 54 24 1 170 222 45 98 2.99e-12 67.0
MS.gene066712.t1 MTR_7g088070 51.786 56 26 1 168 222 48 103 3.60e-12 65.9
MS.gene066712.t1 MTR_5g014040 34.940 83 54 0 169 251 310 392 3.75e-12 67.0
MS.gene066712.t1 MTR_7g115530 47.222 72 36 2 170 239 218 289 5.90e-12 65.5
MS.gene066712.t1 MTR_7g115530 40.659 91 51 3 170 258 218 307 7.43e-12 65.5
MS.gene066712.t1 MTR_7g115530 50.000 64 30 2 170 231 218 281 1.47e-11 64.7
MS.gene066712.t1 MTR_2g027860 55.556 54 23 1 170 222 49 102 1.66e-11 64.7
MS.gene066712.t1 MTR_7g115530 40.659 91 51 3 170 258 218 307 1.66e-11 65.1
MS.gene066712.t1 MTR_7g089010 48.276 58 30 0 169 226 90 147 1.69e-11 64.3
MS.gene066712.t1 MTR_4g098870 48.148 54 28 0 169 222 309 362 1.86e-11 65.1
MS.gene066712.t1 MTR_4g098870 48.148 54 28 0 169 222 309 362 1.94e-11 65.1
MS.gene066712.t1 MTR_4g098870 48.148 54 28 0 169 222 329 382 2.13e-11 65.1
MS.gene066712.t1 MTR_4g098870 48.148 54 28 0 169 222 348 401 2.14e-11 65.1
MS.gene066712.t1 MTR_4g098870 48.148 54 28 0 169 222 329 382 2.22e-11 64.7
MS.gene066712.t1 MTR_4g064730 41.250 80 45 2 147 226 110 187 3.24e-11 63.5
MS.gene066712.t1 MTR_1g093080 45.122 82 35 3 151 229 199 273 3.27e-11 63.9
MS.gene066712.t1 MTR_1g080330 42.857 84 45 3 161 242 165 247 3.38e-11 63.2
MS.gene066712.t1 MTR_7g093030 42.466 73 40 2 167 238 209 280 3.49e-11 63.2
MS.gene066712.t1 MTR_1g080330 47.059 68 34 2 166 231 171 238 8.12e-11 62.8
MS.gene066712.t1 MTR_7g093030 47.368 57 29 1 167 222 209 265 8.63e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066712.t1 AT2G01760 38.783 263 120 6 7 232 4 262 7.26e-55 183
MS.gene066712.t1 AT1G67710 39.700 267 125 9 11 243 8 272 3.38e-53 182
MS.gene066712.t1 AT3G16857 36.577 298 134 8 10 252 33 330 8.52e-51 178
MS.gene066712.t1 AT3G16857 36.577 298 134 8 10 252 33 330 1.49e-50 178
MS.gene066712.t1 AT2G01760 35.052 291 120 7 7 232 4 290 6.53e-49 168
MS.gene066712.t1 AT4G31920 36.910 233 137 2 10 232 13 245 1.13e-46 165
MS.gene066712.t1 AT2G25180 38.750 240 123 6 10 226 13 251 2.82e-43 157
MS.gene066712.t1 AT3G16857 35.521 259 112 8 49 252 2 260 7.61e-40 147
MS.gene066712.t1 AT5G58080 37.008 254 126 7 10 236 14 260 1.09e-38 144
MS.gene066712.t1 AT1G67710 39.713 209 91 8 68 243 1 207 1.21e-35 134
MS.gene066712.t1 AT2G01760 36.318 201 88 5 68 232 1 197 8.91e-35 129
MS.gene066712.t1 AT5G49240 31.356 236 137 5 9 219 37 272 1.33e-33 125
MS.gene066712.t1 AT3G62670 30.258 271 159 9 28 272 53 319 7.56e-32 123
MS.gene066712.t1 AT1G49190 27.734 256 154 6 10 234 30 285 5.13e-28 112
MS.gene066712.t1 AT1G67710 38.095 189 82 8 88 243 12 198 1.41e-26 108
MS.gene066712.t1 AT5G07210 28.148 270 140 7 14 231 16 283 1.46e-26 110
MS.gene066712.t1 AT5G07210 27.338 278 140 8 14 231 16 291 5.18e-24 102
MS.gene066712.t1 AT2G27070 25.758 264 139 8 14 222 16 277 2.26e-20 92.0
MS.gene066712.t1 AT1G49190 32.258 124 83 1 10 132 30 153 4.08e-17 82.4
MS.gene066712.t1 AT5G29000 44.086 93 47 3 148 238 212 301 1.81e-15 75.9
MS.gene066712.t1 AT5G29000 42.000 100 53 3 148 245 212 308 2.26e-15 76.6
MS.gene066712.t1 AT5G29000 42.000 100 53 3 148 245 169 265 2.69e-15 76.3
MS.gene066712.t1 AT5G29000 42.000 100 53 3 148 245 169 265 2.69e-15 76.3
MS.gene066712.t1 AT5G44190 48.333 60 31 0 167 226 144 203 1.28e-13 71.2
MS.gene066712.t1 AT5G44190 48.333 60 31 0 167 226 144 203 1.36e-13 70.9
MS.gene066712.t1 AT3G04030 55.556 54 23 1 170 222 45 98 4.47e-13 69.7
MS.gene066712.t1 AT3G04030 55.556 54 23 1 170 222 45 98 5.01e-13 69.3
MS.gene066712.t1 AT4G28610 47.126 87 40 4 170 252 225 309 8.03e-13 68.9
MS.gene066712.t1 AT5G18240 55.556 54 23 1 170 222 45 98 9.20e-13 68.6
MS.gene066712.t1 AT5G18240 55.556 54 23 1 170 222 45 98 9.20e-13 68.6
MS.gene066712.t1 AT5G18240 55.556 54 23 1 170 222 45 98 1.04e-12 68.6
MS.gene066712.t1 AT5G18240 55.556 54 23 1 170 222 45 98 1.05e-12 68.6
MS.gene066712.t1 AT5G18240 55.556 54 23 1 170 222 45 98 1.05e-12 68.6
MS.gene066712.t1 AT2G01060 45.783 83 42 2 159 238 4 86 1.43e-12 67.4
MS.gene066712.t1 AT5G59570 42.105 76 41 1 151 226 125 197 1.58e-12 67.4
MS.gene066712.t1 AT5G59570 42.105 76 41 1 151 226 125 197 1.58e-12 67.4
MS.gene066712.t1 AT3G04450 48.276 58 29 1 166 222 234 291 1.97e-12 67.0
MS.gene066712.t1 AT3G04030 52.830 53 25 0 170 222 45 97 2.86e-12 67.4
MS.gene066712.t1 AT3G46640 43.210 81 43 2 146 226 123 200 3.17e-12 66.6
MS.gene066712.t1 AT3G46640 43.210 81 43 2 146 226 123 200 3.17e-12 66.6
MS.gene066712.t1 AT3G46640 43.210 81 43 2 146 226 123 200 3.47e-12 66.6
MS.gene066712.t1 AT3G04450 48.276 58 29 1 166 222 234 291 4.14e-12 67.0
MS.gene066712.t1 AT3G04450 48.276 58 29 1 166 222 194 251 4.92e-12 66.6
MS.gene066712.t1 AT3G04450 48.276 58 29 1 166 222 194 251 4.92e-12 66.6
MS.gene066712.t1 AT2G20570 46.552 58 31 0 169 226 152 209 7.30e-12 66.2
MS.gene066712.t1 AT1G69580 51.852 54 25 1 170 222 34 87 8.06e-12 65.5
MS.gene066712.t1 AT1G69580 51.852 54 25 1 170 222 34 87 8.56e-12 65.5
MS.gene066712.t1 AT2G40970 46.552 58 31 0 169 226 104 161 1.14e-11 64.3
MS.gene066712.t1 AT3G10760 48.276 58 30 0 169 226 104 161 1.30e-11 65.1
MS.gene066712.t1 AT5G05090 48.276 58 30 0 169 226 80 137 1.58e-11 63.9
MS.gene066712.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.98e-11 65.1
MS.gene066712.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.98e-11 65.1
MS.gene066712.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.98e-11 65.1
MS.gene066712.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.98e-11 65.1
MS.gene066712.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.98e-11 65.1
MS.gene066712.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.98e-11 65.1
MS.gene066712.t1 AT4G18020 36.842 76 45 1 152 227 281 353 2.22e-11 64.7
MS.gene066712.t1 AT4G18020 36.842 76 45 1 152 227 281 353 2.22e-11 64.7
MS.gene066712.t1 AT4G18020 36.842 76 45 1 152 227 281 353 2.22e-11 64.7
MS.gene066712.t1 AT4G18020 36.842 76 45 1 152 227 281 353 2.22e-11 64.7
MS.gene066712.t1 AT4G18020 36.842 76 45 1 152 227 281 353 2.22e-11 64.7
MS.gene066712.t1 AT2G06020 29.221 154 92 3 116 260 32 177 4.38e-11 63.2
MS.gene066712.t1 AT2G20570 47.273 55 29 0 172 226 171 225 4.79e-11 63.5
MS.gene066712.t1 AT3G24120 48.529 68 31 2 170 233 41 108 7.58e-11 62.4
MS.gene066712.t1 AT3G24120 48.529 68 31 2 170 233 41 108 7.93e-11 62.4
MS.gene066712.t1 AT4G04605 46.970 66 33 2 168 231 12 77 9.83e-11 61.2

Find 60 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATTGTGTCGCAGAATTC+AGG 0.268438 4.3:-76400290 None:intergenic
ACATATTGCCAGCCATTTAC+AGG 0.282271 4.3:+76399784 MS.gene066712:CDS
TTCAGTTACCAAATGTAGTA+TGG 0.293347 4.3:+76399059 MS.gene066712:intron
CAACGACATTGGGTGGAAAT+TGG 0.345706 4.3:+76400330 MS.gene066712:CDS
TGAGTTGTAGGGTAACATTT+TGG 0.376494 4.3:-76399176 None:intergenic
CACATATTGGACTAAGCCTT+TGG 0.377509 4.3:+76399385 MS.gene066712:CDS
GATAAATCATCATCAACATA+TGG 0.380393 4.3:-76400402 None:intergenic
TGACAGGAATGTTGAGTTGT+AGG 0.408636 4.3:-76399188 None:intergenic
ATCAGAAGCCATACTACATT+TGG 0.409595 4.3:-76399067 None:intergenic
CTTTCTTTCCAACATGTTGA+AGG 0.419409 4.3:-76399091 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.435563 4.3:+76399561 MS.gene066712:CDS
AAAAGAAACACTTGCAAAGA+AGG 0.435703 4.3:+76399547 MS.gene066712:CDS
CGAGACCGGCTGGGAATGTT+GGG 0.438211 4.3:-76398862 None:intergenic
GGTTCAAGAAAATAGAAAAC+GGG 0.441425 4.3:+76399502 MS.gene066712:CDS
GCAATAACCTCGAGACCGGC+TGG 0.445702 4.3:-76398872 None:intergenic
TTGCGGTGCACAACGACATT+GGG 0.452490 4.3:+76400320 MS.gene066712:CDS
CTTCAACATGTTGGAAAGAA+AGG 0.459586 4.3:+76399092 MS.gene066712:CDS
AATAAATTCTTACTAATGAC+AGG 0.461511 4.3:-76399204 None:intergenic
AATAAAGAATATGAAAATGT+TGG 0.463084 4.3:+76399470 MS.gene066712:CDS
TTCTCGTGACCAAGACAAAC+GGG 0.467708 4.3:-76399570 None:intergenic
AATGTGGGGCTTGCACATAT+TGG 0.467947 4.3:+76399372 MS.gene066712:CDS
TCGAGACCGGCTGGGAATGT+TGG 0.467950 4.3:-76398863 None:intergenic
TCAACATATGGGAACAAGTT+GGG 0.468173 4.3:-76400390 None:intergenic
TGTTGGGTTATCAGTGAAAC+GGG 0.473997 4.3:-76398846 None:intergenic
ATTGCGGTGCACAACGACAT+TGG 0.484724 4.3:+76400319 MS.gene066712:CDS
AAAAGTGTTGCTCATAATGT+TGG 0.490442 4.3:-76400358 None:intergenic
AAGTCATAAACATCCATGTC+TGG 0.491105 4.3:-76399150 None:intergenic
CACTTTCCTCAACTCTATTG+AGG 0.492388 4.3:+76398910 MS.gene066712:CDS
TGGTTCAAGAAAATAGAAAA+CGG 0.501089 4.3:+76399501 MS.gene066712:CDS
AGGAAAGAATCATACTATCA+AGG 0.510494 4.3:-76399631 None:intergenic
ATTATGAAGTCTATTGAATG+TGG 0.513075 4.3:+76399356 MS.gene066712:CDS
ATGTTGGGTTATCAGTGAAA+CGG 0.516952 4.3:-76398847 None:intergenic
TGTAATCGATATCATTATCA+AGG 0.517908 4.3:+76398938 MS.gene066712:CDS
GACAGGAATGTTGAGTTGTA+GGG 0.519779 4.3:-76399187 None:intergenic
ATTCTCGTGACCAAGACAAA+CGG 0.526657 4.3:-76399571 None:intergenic
AAAATGAACCTGTAAATGGC+TGG 0.527802 4.3:-76399792 None:intergenic
TTCTGATGCCTTCAACATGT+TGG 0.527887 4.3:+76399083 MS.gene066712:CDS
CATGTTGCAAGAATTAGAAA+CGG 0.528421 4.3:+76399437 MS.gene066712:CDS
TTATGAAGTCTATTGAATGT+GGG 0.532174 4.3:+76399357 MS.gene066712:CDS
ATATGAAAATGTTGGAAGCT+CGG 0.535763 4.3:+76399478 MS.gene066712:CDS
AGAAAACTCTGGTCATGCTC+CGG 0.537353 4.3:-76400267 None:intergenic
CTACGTGCAGTCAATCAACT+AGG 0.547867 4.3:+76399608 MS.gene066712:CDS
ATAAATCATCATCAACATAT+GGG 0.548211 4.3:-76400401 None:intergenic
AGCTCAACAGAAGAGACAAA+AGG 0.548661 4.3:+76400177 MS.gene066712:CDS
AATTGCAATAACCTCGAGAC+CGG 0.556151 4.3:-76398876 None:intergenic
ATCAACATATGGGAACAAGT+TGG 0.563419 4.3:-76400391 None:intergenic
CGGTGCACAACGACATTGGG+TGG 0.580466 4.3:+76400323 MS.gene066712:CDS
AGAAGCTACTCACTCAATGC+CGG 0.591913 4.3:+76400248 MS.gene066712:CDS
TATGAAGTCTATTGAATGTG+GGG 0.598969 4.3:+76399358 MS.gene066712:CDS
CAATAACCTCGAGACCGGCT+GGG 0.602751 4.3:-76398871 None:intergenic
AACATTCCCAGCCGGTCTCG+AGG 0.602762 4.3:+76398865 MS.gene066712:CDS
CAGAATTCAGGAGAAAACTC+TGG 0.602905 4.3:-76400278 None:intergenic
CAACAGAAGAGACAAAAGGT+AGG 0.617016 4.3:+76400181 MS.gene066712:CDS
ACACAATGTCGCGACAATTG+CGG 0.621469 4.3:+76400303 MS.gene066712:CDS
ACATCTCCTCAATAGAGTTG+AGG 0.625514 4.3:-76398916 None:intergenic
TGATGCTCATATGCCAGACA+TGG 0.629107 4.3:+76399137 MS.gene066712:CDS
GATAACCCAACATTCCCAGC+CGG 0.655532 4.3:+76398857 MS.gene066712:CDS
TGAAAATGTTGGAAGCTCGG+TGG 0.688521 4.3:+76399481 MS.gene066712:CDS
ATGATTTATCATCACTTGTG+TGG 0.696325 4.3:+76400415 MS.gene066712:CDS
GTTCAAGAAAATAGAAAACG+GGG 0.742093 4.3:+76399503 MS.gene066712:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGAATATGAAAATGT+TGG + chr4.3:76399470-76399489 MS.gene066712:CDS 15.0%
!!! AATATGTTCTTTTTTCATAT+CGG - chr4.3:76399771-76399790 None:intergenic 15.0%
!!! TGATTTTAAACTGAAATTTA+TGG + chr4.3:76400092-76400111 MS.gene066712:intron 15.0%
!! AAAAGATGAAAACTTGAATA+AGG - chr4.3:76398981-76399000 None:intergenic 20.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4.3:76399207-76399226 None:intergenic 20.0%
!! AGCATAGTAAAAAAGTAATT+AGG - chr4.3:76399654-76399673 None:intergenic 20.0%
!! ATAAATCATCATCAACATAT+GGG - chr4.3:76400404-76400423 None:intergenic 20.0%
!! CAATATAAGAATAAACAAAC+AGG - chr4.3:76399034-76399053 None:intergenic 20.0%
!!! AAAAAAGATTCTGAAAATCA+TGG + chr4.3:76399739-76399758 MS.gene066712:CDS 20.0%
!!! ATATGTTCTTTTTTCATATC+GGG - chr4.3:76399770-76399789 None:intergenic 20.0%
!!! ATATTTCTTTTTTGTAGTGA+TGG + chr4.3:76399310-76399329 MS.gene066712:intron 20.0%
!!! ATGATTTTCAGAATCTTTTT+TGG - chr4.3:76399740-76399759 None:intergenic 20.0%
!!! ATGTTTTTCTCTTAATTTTC+AGG + chr4.3:76399707-76399726 MS.gene066712:intron 20.0%
!!! ATTCAAGTTTTCATCTTTTT+TGG + chr4.3:76398982-76399001 MS.gene066712:intron 20.0%
!!! TGTTTTTCTCTTAATTTTCA+GGG + chr4.3:76399708-76399727 MS.gene066712:intron 20.0%
!!! TTTTGCAGAAATATAGAAAT+CGG + chr4.3:76400148-76400167 MS.gene066712:intron 20.0%
! AGTAGTATATTATCAACAAG+AGG - chr4.3:76400061-76400080 None:intergenic 25.0%
! ATTATGAAGTCTATTGAATG+TGG + chr4.3:76399356-76399375 MS.gene066712:CDS 25.0%
! GATAAATCATCATCAACATA+TGG - chr4.3:76400405-76400424 None:intergenic 25.0%
! TACAAAAATGAACCTGTAAA+TGG - chr4.3:76399799-76399818 None:intergenic 25.0%
! TGGTTCAAGAAAATAGAAAA+CGG + chr4.3:76399501-76399520 MS.gene066712:CDS 25.0%
! TGTAATCGATATCATTATCA+AGG + chr4.3:76398938-76398957 MS.gene066712:CDS 25.0%
! TTATGAAGTCTATTGAATGT+GGG + chr4.3:76399357-76399376 MS.gene066712:CDS 25.0%
!! CTTCATAATAGAACTTTTTG+TGG - chr4.3:76399345-76399364 None:intergenic 25.0%
!! GTTTTTCTCTTAATTTTCAG+GGG + chr4.3:76399709-76399728 MS.gene066712:intron 25.0%
!!! AAGTCAAATTTTGTTTCAAG+AGG - chr4.3:76400228-76400247 None:intergenic 25.0%
AAAAGAAACACTTGCAAAGA+AGG + chr4.3:76399547-76399566 MS.gene066712:CDS 30.0%
AAAAGATAACTGACAAACCT+AGG - chr4.3:76399875-76399894 None:intergenic 30.0%
AGGAAAGAATCATACTATCA+AGG - chr4.3:76399634-76399653 None:intergenic 30.0%
ATATGAAAATGTTGGAAGCT+CGG + chr4.3:76399478-76399497 MS.gene066712:CDS 30.0%
ATGATTTATCATCACTTGTG+TGG + chr4.3:76400415-76400434 MS.gene066712:CDS 30.0%
CATGTTGCAAGAATTAGAAA+CGG + chr4.3:76399437-76399456 MS.gene066712:CDS 30.0%
GGTTCAAGAAAATAGAAAAC+GGG + chr4.3:76399502-76399521 MS.gene066712:CDS 30.0%
GTTCAAGAAAATAGAAAACG+GGG + chr4.3:76399503-76399522 MS.gene066712:CDS 30.0%
TATGAAGTCTATTGAATGTG+GGG + chr4.3:76399358-76399377 MS.gene066712:CDS 30.0%
TGATTTACAACAACAGACTT+AGG - chr4.3:76399899-76399918 None:intergenic 30.0%
TTCAGTTACCAAATGTAGTA+TGG + chr4.3:76399059-76399078 MS.gene066712:intron 30.0%
TTGAATAACATGCAAGACTA+AGG - chr4.3:76399937-76399956 None:intergenic 30.0%
!! AAAAGTGTTGCTCATAATGT+TGG - chr4.3:76400361-76400380 None:intergenic 30.0%
AAAAAGAGGTCAAGATCATG+TGG + chr4.3:76399411-76399430 MS.gene066712:CDS 35.0%
AAAATGAACCTGTAAATGGC+TGG - chr4.3:76399795-76399814 None:intergenic 35.0%
AAGTCATAAACATCCATGTC+TGG - chr4.3:76399153-76399172 None:intergenic 35.0%
AGAGGATGTATAGAAGCTAA+TGG - chr4.3:76400043-76400062 None:intergenic 35.0%
AGTAGTGAATTATGCAGTCA+TGG + chr4.3:76399973-76399992 MS.gene066712:intron 35.0%
ATCAACATATGGGAACAAGT+TGG - chr4.3:76400394-76400413 None:intergenic 35.0%
ATCAGAAGCCATACTACATT+TGG - chr4.3:76399070-76399089 None:intergenic 35.0%
ATGTTGGGTTATCAGTGAAA+CGG - chr4.3:76398850-76398869 None:intergenic 35.0%
CTTCAACATGTTGGAAAGAA+AGG + chr4.3:76399092-76399111 MS.gene066712:CDS 35.0%
CTTTCTTTCCAACATGTTGA+AGG - chr4.3:76399094-76399113 None:intergenic 35.0%
TAAGCCTTTGGTTGAAAAAG+AGG + chr4.3:76399397-76399416 MS.gene066712:CDS 35.0%
TCAACATATGGGAACAAGTT+GGG - chr4.3:76400393-76400412 None:intergenic 35.0%
! CTTTGCAAGTGTTTCTTTTG+AGG - chr4.3:76399546-76399565 None:intergenic 35.0%
! TGAGTTGTAGGGTAACATTT+TGG - chr4.3:76399179-76399198 None:intergenic 35.0%
!!! TTGACCTCTTTTTCAACCAA+AGG - chr4.3:76399404-76399423 None:intergenic 35.0%
AATTGCAATAACCTCGAGAC+CGG - chr4.3:76398879-76398898 None:intergenic 40.0%
ACATATTGCCAGCCATTTAC+AGG + chr4.3:76399784-76399803 MS.gene066712:CDS 40.0%
ACATCTCCTCAATAGAGTTG+AGG - chr4.3:76398919-76398938 None:intergenic 40.0%
ACCTAAGTCTATAGTTGCCT+AGG + chr4.3:76399855-76399874 MS.gene066712:intron 40.0%
ACCTAGGCAACTATAGACTT+AGG - chr4.3:76399859-76399878 None:intergenic 40.0%
AGCTCAACAGAAGAGACAAA+AGG + chr4.3:76400177-76400196 MS.gene066712:CDS 40.0%
ATTCTCGTGACCAAGACAAA+CGG - chr4.3:76399574-76399593 None:intergenic 40.0%
CAACAGAAGAGACAAAAGGT+AGG + chr4.3:76400181-76400200 MS.gene066712:CDS 40.0%
CACATATTGGACTAAGCCTT+TGG + chr4.3:76399385-76399404 MS.gene066712:CDS 40.0%
CACTTTCCTCAACTCTATTG+AGG + chr4.3:76398910-76398929 MS.gene066712:CDS 40.0%
CAGAATTCAGGAGAAAACTC+TGG - chr4.3:76400281-76400300 None:intergenic 40.0%
TGTTGGGTTATCAGTGAAAC+GGG - chr4.3:76398849-76398868 None:intergenic 40.0%
TTCTGATGCCTTCAACATGT+TGG + chr4.3:76399083-76399102 MS.gene066712:CDS 40.0%
! GACAGGAATGTTGAGTTGTA+GGG - chr4.3:76399190-76399209 None:intergenic 40.0%
! TGACAGGAATGTTGAGTTGT+AGG - chr4.3:76399191-76399210 None:intergenic 40.0%
AATGTGGGGCTTGCACATAT+TGG + chr4.3:76399372-76399391 MS.gene066712:CDS 45.0%
ACACAATGTCGCGACAATTG+CGG + chr4.3:76400303-76400322 MS.gene066712:CDS 45.0%
AGAAAACTCTGGTCATGCTC+CGG - chr4.3:76400270-76400289 None:intergenic 45.0%
AGAAGCTACTCACTCAATGC+CGG + chr4.3:76400248-76400267 MS.gene066712:CDS 45.0%
CAACGACATTGGGTGGAAAT+TGG + chr4.3:76400330-76400349 MS.gene066712:CDS 45.0%
CTACGTGCAGTCAATCAACT+AGG + chr4.3:76399608-76399627 MS.gene066712:CDS 45.0%
GACATTGTGTCGCAGAATTC+AGG - chr4.3:76400293-76400312 None:intergenic 45.0%
TGAAAATGTTGGAAGCTCGG+TGG + chr4.3:76399481-76399500 MS.gene066712:CDS 45.0%
TGATGCTCATATGCCAGACA+TGG + chr4.3:76399137-76399156 MS.gene066712:CDS 45.0%
TTCTCGTGACCAAGACAAAC+GGG - chr4.3:76399573-76399592 None:intergenic 45.0%
ATTGCGGTGCACAACGACAT+TGG + chr4.3:76400319-76400338 MS.gene066712:CDS 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4.3:76399561-76399580 MS.gene066712:CDS 50.0%
GATAACCCAACATTCCCAGC+CGG + chr4.3:76398857-76398876 MS.gene066712:CDS 50.0%
TTGCGGTGCACAACGACATT+GGG + chr4.3:76400320-76400339 MS.gene066712:CDS 50.0%
CAATAACCTCGAGACCGGCT+GGG - chr4.3:76398874-76398893 None:intergenic 55.0%
AACATTCCCAGCCGGTCTCG+AGG + chr4.3:76398865-76398884 MS.gene066712:CDS 60.0%
CGAGACCGGCTGGGAATGTT+GGG - chr4.3:76398865-76398884 None:intergenic 60.0%
CGGTGCACAACGACATTGGG+TGG + chr4.3:76400323-76400342 MS.gene066712:CDS 60.0%
GCAATAACCTCGAGACCGGC+TGG - chr4.3:76398875-76398894 None:intergenic 60.0%
TCGAGACCGGCTGGGAATGT+TGG - chr4.3:76398866-76398885 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 76398842 76400440 76398842 ID=MS.gene066712
chr4.3 mRNA 76398842 76400440 76398842 ID=MS.gene066712.t1;Parent=MS.gene066712
chr4.3 exon 76398842 76398959 76398842 ID=MS.gene066712.t1.exon1;Parent=MS.gene066712.t1
chr4.3 CDS 76398842 76398959 76398842 ID=cds.MS.gene066712.t1;Parent=MS.gene066712.t1
chr4.3 exon 76399064 76399213 76399064 ID=MS.gene066712.t1.exon2;Parent=MS.gene066712.t1
chr4.3 CDS 76399064 76399213 76399064 ID=cds.MS.gene066712.t1;Parent=MS.gene066712.t1
chr4.3 exon 76399327 76399638 76399327 ID=MS.gene066712.t1.exon3;Parent=MS.gene066712.t1
chr4.3 CDS 76399327 76399638 76399327 ID=cds.MS.gene066712.t1;Parent=MS.gene066712.t1
chr4.3 exon 76399729 76399805 76399729 ID=MS.gene066712.t1.exon4;Parent=MS.gene066712.t1
chr4.3 CDS 76399729 76399805 76399729 ID=cds.MS.gene066712.t1;Parent=MS.gene066712.t1
chr4.3 exon 76400156 76400440 76400156 ID=MS.gene066712.t1.exon5;Parent=MS.gene066712.t1
chr4.3 CDS 76400156 76400440 76400156 ID=cds.MS.gene066712.t1;Parent=MS.gene066712.t1
Gene Sequence

>MS.gene066712

ATGGCCCGTTTCACTGATAACCCAACATTCCCAGCCGGTCTCGAGGTTATTGCAATTGATCATGACATCACTTTCCTCAACTCTATTGAGGAGATGTGTAATCGATATCATTATCAAGGTTAGTTACTGAATCATACCTTATTCAAGTTTTCATCTTTTTTGGTAAAAATTCTTACTTTTATATGTATTCCTGTTTGTTTATTCTTATATTGTTTATTTCAGTTACCAAATGTAGTATGGCTTCTGATGCCTTCAACATGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTGATGCTCATATGCCAGACATGGATGTTTATGACTTTGTCCAAAATGTTACCCTACAACTCAACATTCCTGTCATTAGTAAGAATTTATTCAACTTTTTCATTAAAATTCATTAGTTACACATTTCACATAATTTATTCATATATATGTACTTAAGAAGTGTGAATTGACATTATATTTCTTTTTTGTAGTGATGGCTGTTGATTCCACAAAAAGTTCTATTATGAAGTCTATTGAATGTGGGGCTTGCACATATTGGACTAAGCCTTTGGTTGAAAAAGAGGTCAAGATCATGTGGCAACATGTTGCAAGAATTAGAAACGGTTTGATTGAAAATAAAGAATATGAAAATGTTGGAAGCTCGGTGGTTCAAGAAAATAGAAAACGGGGTAGAGAAGATGCTAATGCCTCAAAAGAAACACTTGCAAAGAAGGCCCGTTTGTCTTGGTCACGAGAATTGCATCAGCAGTTTCTACGTGCAGTCAATCAACTAGGCCTTGATAGTATGATTCTTTCCTAATTACTTTTTTACTATGCTTTGTTGCATTCTTCAACAGTTGAACTTATGTGCATGTTTTTCTCTTAATTTTCAGGGGCAACGCCAAAAAAGATTCTGAAAATCATGGATGTTCCCGATATGAAAAAAGAACATATTGCCAGCCATTTACAGGTTCATTTTTGTATTAATTTTTGTTGATTCTGTAAATTTGATATATTTTACCTAAGTCTATAGTTGCCTAGGTTTGTCAGTTATCTTTTACCTAAGTCTGTTGTTGTAAATCATTTATGTTGTTTATACCTTAGTCTTGCATGTTATTCAAACTATTGAAGTTGTAAAGTAGTGAATTATGCAGTCATGGCAGATCTTGAAGTCTGAGTTTTAGAATAGAATATAAGTATTTCTCCATTAGCTTCTATACATCCTCTTGTTGATAATATACTACTTGTCTTTTGTGTGATTTTAAACTGAAATTTATGGAGTATTATTATTAACATTGTAACTGCAATGTTGTTTTGCAGAAATATAGAAATCGGTTAAAGAGCTCAACAGAAGAGACAAAAGGTAGGATGAAAAAATCAAAGACGAGTCCTCTTGAAACAAAATTTGACTTAGAAGCTACTCACTCAATGCCGGAGCATGACCAGAGTTTTCTCCTGAATTCTGCGACACAATGTCGCGACAATTGCGGTGCACAACGACATTGGGTGGAAATTGGTGAATCCAACATTATGAGCAACACTTTTTCAGACTTGCCCAACTTGTTCCCATATGTTGATGATGATTTATCATCACTTGTGTGGTGA
Protein sequence

>MS.gene066712.t1

MARFTDNPTFPAGLEVIAIDHDITFLNSIEEMCNRYHYQVTKCSMASDAFNMLERKDCFDVMLIDAHMPDMDVYDFVQNVTLQLNIPVIMMAVDSTKSSIMKSIECGACTYWTKPLVEKEVKIMWQHVARIRNGLIENKEYENVGSSVVQENRKRGREDANASKETLAKKARLSWSRELHQQFLRAVNQLGLDRATPKKILKIMDVPDMKKEHIASHLQKYRNRLKSSTEETKGRMKKSKTSPLETKFDLEATHSMPEHDQSFLLNSATQCRDNCGAQRHWVEIGESNIMSNTFSDLPNLFPYVDDDLSSLVW