AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066732

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066732.t1 MTR_5g015480 88.649 185 21 0 1 185 1 185 1.31e-110 337
MS.gene066732.t1 MTR_0402s0040 51.964 331 85 6 416 746 200 456 5.18e-89 288
MS.gene066732.t1 MTR_0402s0040 69.500 200 56 2 36 231 2 200 5.67e-86 280
MS.gene066732.t1 MTR_6g017200 71.074 121 31 1 36 152 34 154 2.56e-50 173
MS.gene066732.t1 MTR_2g070590 69.748 119 36 0 40 158 1 119 3.67e-50 171
MS.gene066732.t1 MTR_7g113010 56.875 160 51 2 26 181 423 568 1.18e-45 172
MS.gene066732.t1 MTR_1g057920 64.228 123 40 1 27 145 1 123 4.95e-44 154
MS.gene066732.t1 MTR_1g086270 81.250 80 15 0 351 430 1 80 3.07e-42 149
MS.gene066732.t1 MTR_6g051425 30.952 294 181 6 63 337 24 314 1.20e-40 152
MS.gene066732.t1 MTR_0558s0020 30.769 247 152 4 70 297 50 296 2.49e-35 136
MS.gene066732.t1 MTR_0137s0040 68.182 88 24 1 25 108 1 88 5.71e-34 126
MS.gene066732.t1 MTR_1g070355 30.526 285 178 7 21 293 35 311 2.06e-31 130
MS.gene066732.t1 MTR_8g446020 31.694 183 124 1 116 297 74 256 2.61e-30 120
MS.gene066732.t1 MTR_6g090505 32.967 182 121 1 116 296 49 230 1.40e-27 115
MS.gene066732.t1 MTR_1g050985 80.000 60 12 0 126 185 39 98 6.08e-27 105
MS.gene066732.t1 MTR_7g074790 43.023 172 76 5 56 226 10 160 6.64e-27 110
MS.gene066732.t1 MTR_4g035820 31.939 263 166 8 48 301 66 324 2.32e-25 112
MS.gene066732.t1 MTR_4g102720 35.119 168 73 3 56 223 7 138 2.71e-23 99.4
MS.gene066732.t1 MTR_0291s0060 63.014 73 23 1 38 106 13 85 1.45e-22 92.8
MS.gene066732.t1 MTR_5g076070 27.236 246 167 5 46 285 43 282 7.81e-22 96.7
MS.gene066732.t1 MTR_1g083640 26.991 226 146 4 62 268 57 282 2.06e-21 95.5
MS.gene066732.t1 MTR_7g446520 29.796 245 154 4 59 302 91 318 2.17e-21 96.7
MS.gene066732.t1 MTR_4g067290 46.296 108 52 3 56 161 56 159 6.59e-21 92.8
MS.gene066732.t1 MTR_4g067290 46.296 108 52 3 56 161 56 159 6.94e-21 92.8
MS.gene066732.t1 MTR_4g067290 46.296 108 52 3 56 161 76 179 1.00e-20 92.8
MS.gene066732.t1 MTR_4g067290 46.296 108 52 3 56 161 74 177 1.01e-20 92.8
MS.gene066732.t1 MTR_4g067290 46.296 108 52 3 56 161 76 179 1.01e-20 92.8
MS.gene066732.t1 MTR_4g067290 46.296 108 52 3 56 161 116 219 2.14e-20 92.8
MS.gene066732.t1 MTR_5g039760 36.224 196 95 7 60 252 14 182 5.12e-20 89.0
MS.gene066732.t1 MTR_2g098920 35.294 153 88 5 19 165 44 191 1.18e-19 90.1
MS.gene066732.t1 MTR_1g091013 67.857 56 18 0 69 124 14 69 9.91e-18 78.6
MS.gene066732.t1 MTR_6g022080 30.519 154 104 2 62 213 91 243 8.05e-17 81.3
MS.gene066732.t1 MTR_7g061980 41.176 119 66 3 50 167 4 119 1.12e-16 77.0
MS.gene066732.t1 MTR_0337s0040 27.703 148 106 1 116 262 75 222 1.23e-15 77.4
MS.gene066732.t1 MTR_7g446480 32.955 176 115 3 132 305 140 314 1.60e-15 79.0
MS.gene066732.t1 MTR_1g008290 32.653 147 93 3 108 253 3 144 2.26e-15 74.3
MS.gene066732.t1 MTR_6g022070 28.161 174 120 1 351 524 1 169 3.63e-15 77.8
MS.gene066732.t1 MTR_7g446510 32.386 176 116 3 132 305 155 329 4.25e-15 77.8
MS.gene066732.t1 MTR_3g450850 24.797 246 173 5 62 296 53 297 3.03e-14 75.5
MS.gene066732.t1 MTR_5g045210 38.202 89 55 0 588 676 20 108 6.13e-14 71.6
MS.gene066732.t1 MTR_3g006370 39.048 105 62 2 56 159 50 153 7.66e-14 72.0
MS.gene066732.t1 MTR_2g099430 40.909 88 50 1 80 167 27 112 1.63e-13 68.2
MS.gene066732.t1 MTR_1g019850 30.488 164 104 3 34 194 45 201 8.40e-13 69.3
MS.gene066732.t1 MTR_2g014100 23.383 201 132 4 62 240 50 250 1.23e-12 68.9
MS.gene066732.t1 MTR_2g098880 32.414 145 92 4 42 182 59 201 1.24e-12 69.3
MS.gene066732.t1 MTR_2g098880 34.351 131 80 4 56 182 61 189 1.49e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066732.t1 AT4G38180 34.390 631 402 7 56 677 73 700 9.25e-121 381
MS.gene066732.t1 AT4G15090 31.731 624 412 7 56 673 51 666 4.47e-102 333
MS.gene066732.t1 AT4G15090 31.731 624 412 7 56 673 51 666 4.47e-102 333
MS.gene066732.t1 AT4G15090 31.731 624 412 7 56 673 51 666 4.47e-102 333
MS.gene066732.t1 AT4G15090 31.731 624 412 7 56 673 51 666 4.47e-102 333
MS.gene066732.t1 AT4G15090 31.731 624 412 7 56 673 51 666 4.47e-102 333
MS.gene066732.t1 AT3G22170 32.472 619 369 11 56 656 70 657 8.61e-97 319
MS.gene066732.t1 AT3G22170 32.472 619 369 11 56 656 70 657 8.61e-97 319
MS.gene066732.t1 AT1G76320 31.485 613 399 12 60 668 1 596 2.14e-88 292
MS.gene066732.t1 AT1G76320 31.485 613 399 12 60 668 1 596 1.01e-87 292
MS.gene066732.t1 AT1G76320 31.485 613 399 12 60 668 1 596 1.01e-87 292
MS.gene066732.t1 AT1G76320 31.485 613 399 12 60 668 1 596 1.16e-87 292
MS.gene066732.t1 AT2G27110 31.250 624 382 14 56 669 49 635 4.84e-86 290
MS.gene066732.t1 AT2G27110 31.250 624 382 14 56 669 49 635 4.84e-86 290
MS.gene066732.t1 AT3G06250 28.140 629 394 12 47 668 181 758 4.32e-76 261
MS.gene066732.t1 AT3G06250 28.140 629 394 12 47 668 181 758 4.32e-76 261
MS.gene066732.t1 AT3G06250 28.140 629 394 12 47 668 181 758 4.32e-76 261
MS.gene066732.t1 AT1G10240 29.350 569 388 9 49 608 41 604 1.17e-74 256
MS.gene066732.t1 AT1G10240 29.350 569 388 9 49 608 41 604 1.17e-74 256
MS.gene066732.t1 AT5G18960 29.022 634 390 15 44 668 200 782 1.27e-74 258
MS.gene066732.t1 AT5G18960 33.051 118 65 2 41 158 28 131 2.28e-11 67.8
MS.gene066732.t1 AT5G18960 29.022 634 390 15 44 668 200 782 1.27e-74 258
MS.gene066732.t1 AT5G18960 33.051 118 65 2 41 158 28 131 2.28e-11 67.8
MS.gene066732.t1 AT2G27110 33.183 443 281 8 235 669 55 490 5.56e-74 254
MS.gene066732.t1 AT2G32250 28.019 646 400 12 60 691 42 636 6.94e-73 253
MS.gene066732.t1 AT2G32250 27.752 645 403 11 60 691 1 595 7.79e-73 252
MS.gene066732.t1 AT2G32250 27.752 645 403 11 60 691 1 595 9.76e-73 252
MS.gene066732.t1 AT2G32250 27.752 645 403 11 60 691 42 636 1.27e-72 253
MS.gene066732.t1 AT2G32250 27.752 645 403 11 60 691 42 636 1.27e-72 253
MS.gene066732.t1 AT2G32250 27.752 645 403 11 60 691 42 636 1.41e-72 253
MS.gene066732.t1 AT2G32250 27.752 645 403 11 60 691 42 636 1.41e-72 253
MS.gene066732.t1 AT2G32250 27.752 645 403 11 60 691 42 636 1.41e-72 253
MS.gene066732.t1 AT4G19990 27.910 670 413 10 61 702 26 653 1.20e-65 232
MS.gene066732.t1 AT4G19990 27.910 670 413 10 61 702 26 653 1.20e-65 232
MS.gene066732.t1 AT4G19990 27.910 670 413 10 61 702 26 653 1.98e-65 232
MS.gene066732.t1 AT4G19990 27.910 670 413 10 61 702 26 653 1.98e-65 232
MS.gene066732.t1 AT4G19990 27.910 670 413 10 61 702 26 653 2.04e-65 232
MS.gene066732.t1 AT4G19990 27.910 670 413 10 61 702 40 667 2.32e-65 232
MS.gene066732.t1 AT4G38170 30.526 380 251 5 240 612 27 400 1.30e-61 217
MS.gene066732.t1 AT1G80010 29.372 589 361 15 56 611 95 661 9.58e-61 218
MS.gene066732.t1 AT5G28530 26.589 598 406 12 56 631 55 641 1.14e-60 217
MS.gene066732.t1 AT5G28530 26.821 604 393 13 56 631 55 637 1.58e-60 217
MS.gene066732.t1 AT5G28530 26.821 604 393 13 56 631 55 637 1.58e-60 217
MS.gene066732.t1 AT1G52520 27.875 574 376 14 54 609 83 636 8.91e-59 212
MS.gene066732.t1 AT4G19990 27.358 636 392 10 95 702 3 596 1.11e-57 209
MS.gene066732.t1 AT5G28530 24.074 594 359 13 56 631 55 574 2.68e-41 160
MS.gene066732.t1 AT5G28530 23.810 588 372 12 56 631 55 578 2.88e-41 160
MS.gene066732.t1 AT3G59470 46.226 106 55 2 56 161 70 173 2.04e-21 94.7
MS.gene066732.t1 AT3G59470 46.226 106 55 2 56 161 70 173 2.04e-21 94.7
MS.gene066732.t1 AT3G59470 46.226 106 55 2 56 161 82 185 2.91e-21 94.7
MS.gene066732.t1 AT3G07500 38.760 129 76 2 34 160 9 136 1.75e-15 76.6
MS.gene066732.t1 AT2G43280 36.220 127 74 3 56 181 22 142 6.58e-14 71.6
MS.gene066732.t1 AT4G12850 32.540 126 78 3 56 181 11 129 2.50e-12 65.5
MS.gene066732.t1 AT2G43280 40.659 91 50 3 71 159 2 90 1.25e-11 64.3
MS.gene066732.t1 AT4G12850 34.951 103 63 2 56 158 11 109 2.96e-11 62.4
MS.gene066732.t1 AT4G12850 34.951 103 63 2 56 158 11 109 8.72e-11 62.0

Find 114 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAAATAACAAACATATTT+TGG 0.063618 2.3:+34985137 MS.gene066732:CDS
TCCAACTTGGCTTGAGAAAT+TGG 0.175914 2.3:+34986542 MS.gene066732:CDS
CACAGACAAACAATTGTTTA+TGG 0.199392 2.3:+34985277 MS.gene066732:CDS
ATTCATCAGCCAATACAATT+TGG 0.204201 2.3:-34984901 None:intergenic
TTGTTTGTCTGTGGCTATTA+AGG 0.204260 2.3:-34985267 None:intergenic
GCACAAATGATAAATGATTA+TGG 0.212778 2.3:+34985166 MS.gene066732:CDS
CACAATCCATGAAATGTTTC+TGG 0.237682 2.3:-34985442 None:intergenic
GAAATTTGCTTCGCACTAAA+AGG 0.249768 2.3:+34985011 MS.gene066732:CDS
GGTTAGGATGTAATCAAAAT+TGG 0.289185 2.3:+34986196 MS.gene066732:CDS
ACTAATTCATGAAAATCTTT+TGG 0.310628 2.3:-34984935 None:intergenic
ATTGTATTGGCTGATGAATC+TGG 0.322531 2.3:+34984905 MS.gene066732:CDS
CATTTCATGGATTGTGCACT+TGG 0.329207 2.3:+34985449 MS.gene066732:CDS
TCTTGGAGTGTAAACATTAC+CGG 0.342856 2.3:-34985905 None:intergenic
TAGTGCGAAGCAAATTTCTA+TGG 0.349224 2.3:-34985006 None:intergenic
GACATTCCTCATATTCATCT+TGG 0.362108 2.3:-34985942 None:intergenic
CTTCTGTCTTGACTCATATT+CGG 0.366494 2.3:-34985839 None:intergenic
ACAGACAAACAATTGTTTAT+GGG 0.367661 2.3:+34985278 MS.gene066732:CDS
CAAATAACCATTGAAAAGAA+TGG 0.368518 2.3:-34985327 None:intergenic
TATAGTAGGAAATGTGGTTT+TGG 0.369598 2.3:+34984587 MS.gene066732:CDS
CGTCCGTTGGGAGTATTTGT+TGG 0.371935 2.3:+34985244 MS.gene066732:CDS
TTCATCAGCCAATACAATTT+GGG 0.375471 2.3:-34984900 None:intergenic
GAAAGGGGAACATATTGAAT+TGG 0.379587 2.3:+34986218 MS.gene066732:CDS
GGTGAATGATATCGTGCAAC+AGG 0.381657 2.3:+34984481 MS.gene066732:CDS
AGCAAGGAGCGGATAGTAGT+AGG 0.390919 2.3:+34986024 MS.gene066732:CDS
ATGAGACTCTTCATAAACTT+TGG 0.394637 2.3:-34986343 None:intergenic
CCCACACATTGCTTTGAGAA+AGG 0.396961 2.3:-34985353 None:intergenic
ATTTCACTTGATCGGAAGAT+TGG 0.399360 2.3:+34984770 MS.gene066732:CDS
GTCATGCCAGAAACATTTCA+TGG 0.400517 2.3:+34985436 MS.gene066732:CDS
CACAAATGATAAATGATTAT+GGG 0.407470 2.3:+34985167 MS.gene066732:CDS
AACACCGCGTCTACCCTCTT+TGG 0.411087 2.3:-34984678 None:intergenic
ATAGATGATTCACATGCCTT+AGG 0.411459 2.3:-34985489 None:intergenic
GATTGTGCACTTGGCACATA+AGG 0.422392 2.3:+34985458 MS.gene066732:CDS
TAATGTACCACACTTGGAAA+TGG 0.430430 2.3:+34984523 MS.gene066732:CDS
TACACCTTCAACCATTGGAT+TGG 0.436578 2.3:-34984450 None:intergenic
ATCATTTATCATTTGTGCAT+CGG 0.444064 2.3:-34985161 None:intergenic
ACGTGTGTCGTTTCTACCAT+TGG 0.448381 2.3:-34984225 None:intergenic
TGTTTAAGAAATTTGAAAGA+AGG 0.449252 2.3:+34985973 MS.gene066732:CDS
TCCAATTTCTCAAGCCAAGT+TGG 0.453093 2.3:-34986543 None:intergenic
TAGATGATTCACATGCCTTA+GGG 0.455627 2.3:-34985488 None:intergenic
AGAGCCGAGATTGCTTAGAT+AGG 0.461774 2.3:+34986505 MS.gene066732:CDS
AACAGGATGTTTAGCACGTA+TGG 0.468473 2.3:+34984745 MS.gene066732:CDS
TGCATCTTGATCTGTTAGAA+TGG 0.469730 2.3:-34985389 None:intergenic
GTGGACATTGTTGATCTTGG+TGG 0.470761 2.3:+34986468 MS.gene066732:CDS
TCACCATTCATCACCACCTA+TGG 0.473057 2.3:+34984266 MS.gene066732:CDS
GGAGTTTGAGTCTGAGGCCT+TGG 0.475074 2.3:+34984544 MS.gene066732:CDS
GTTTAAGAAATTTGAAAGAA+GGG 0.482494 2.3:+34985974 MS.gene066732:CDS
ATAAATGATTATGGGTACTT+TGG 0.482723 2.3:+34985175 MS.gene066732:CDS
AATGTGGACATTGTTGATCT+TGG 0.483171 2.3:+34986465 MS.gene066732:CDS
GGAATTCCAAGATGAATATG+AGG 0.483458 2.3:+34985936 MS.gene066732:CDS
AATAAGAATTATCGTCCGTT+GGG 0.484650 2.3:+34985232 MS.gene066732:CDS
GATGTAATCAAAATTGGAAA+GGG 0.486259 2.3:+34986202 MS.gene066732:CDS
ACAAGCATCCCAAATTGTAT+TGG 0.495869 2.3:+34984892 MS.gene066732:CDS
AACAATATATACTAAAACGT+TGG 0.503287 2.3:+34986160 MS.gene066732:CDS
TCACTAAGGTATGGTGAAGT+AGG 0.507546 2.3:+34985040 MS.gene066732:CDS
TCTATGGATGGAGAGTCAAT+TGG 0.511024 2.3:+34986396 MS.gene066732:CDS
TGGACAAGAGATGCAAGGTT+AGG 0.511096 2.3:+34986180 MS.gene066732:CDS
ATCATATAAACATACTCAGC+TGG 0.522562 2.3:-34984842 None:intergenic
GATAGTAGTAGGGAATCATA+TGG 0.523742 2.3:+34986035 MS.gene066732:CDS
CATACTCAGCTGGCAGAAGA+AGG 0.525063 2.3:-34984832 None:intergenic
AAATAAGAATTATCGTCCGT+TGG 0.526037 2.3:+34985231 MS.gene066732:CDS
TGGACATTGTTGATCTTGGT+GGG 0.528035 2.3:+34986469 MS.gene066732:CDS
TGAAACTCATAAAAGAATGA+AGG 0.528219 2.3:-34985100 None:intergenic
TAGTCCTATCTAAGCAATCT+CGG 0.530700 2.3:-34986509 None:intergenic
ACATCCAATCCAATGGTTGA+AGG 0.531051 2.3:+34984446 MS.gene066732:CDS
GTATGGTTATTTCACTTGAT+CGG 0.532614 2.3:+34984762 MS.gene066732:CDS
ATCCCTATGCTTGTACAAGC+CGG 0.533806 2.3:+34985886 MS.gene066732:CDS
GCTCGAGCAGAAACTAGAAC+AGG 0.536024 2.3:+34984728 MS.gene066732:CDS
GCAAGGAGCGGATAGTAGTA+GGG 0.537014 2.3:+34986025 MS.gene066732:CDS
TCAAGATGCAGCAATGGCTA+AGG 0.541612 2.3:+34985402 MS.gene066732:CDS
ACGTGATTACTTAACAAAAG+AGG 0.543276 2.3:+34984706 MS.gene066732:CDS
CTAAAAGGAACCACTCACTA+AGG 0.543419 2.3:+34985026 MS.gene066732:CDS
CAGACAAACAATTGTTTATG+GGG 0.545894 2.3:+34985279 MS.gene066732:CDS
TTACCGGCTTGTACAAGCAT+AGG 0.547981 2.3:-34985889 None:intergenic
ATAATGTTTCTAAAGACTCA+TGG 0.548211 2.3:+34985614 MS.gene066732:CDS
GGAAATGGAGTTTGAGTCTG+AGG 0.549669 2.3:+34984538 MS.gene066732:CDS
AATGAGTATAGTAGGAAATG+TGG 0.549866 2.3:+34984581 MS.gene066732:CDS
ACCTTTCTCAAAGCAATGTG+TGG 0.550009 2.3:+34985352 MS.gene066732:CDS
TCGGAAGATTGGAAAATACA+AGG 0.556421 2.3:+34984781 MS.gene066732:CDS
TGTCCATAGGTGGTGATGAA+TGG 0.557395 2.3:-34984269 None:intergenic
GGATGTAATCAAAATTGGAA+AGG 0.558394 2.3:+34986201 MS.gene066732:CDS
TGTTAGTAATGTACCACACT+TGG 0.560348 2.3:+34984517 MS.gene066732:CDS
AAAGTCTTACCGGTGTCCAT+AGG 0.562691 2.3:-34984282 None:intergenic
ATTGTGCACTTGGCACATAA+GGG 0.563830 2.3:+34985459 MS.gene066732:CDS
TTGCTCAATTTAACTGCTCG+AGG 0.564353 2.3:-34986282 None:intergenic
TGTCGAAAATTTGAGACACA+TGG 0.568497 2.3:+34986078 MS.gene066732:CDS
ATTATAAAATTCATATGCCA+AGG 0.568982 2.3:-34984561 None:intergenic
AACAGATCAAGATGCAGCAA+TGG 0.573110 2.3:+34985396 MS.gene066732:CDS
ATTCTTATTTGTCTTGAATG+TGG 0.574010 2.3:-34985218 None:intergenic
AGGAACCACTCACTAAGGTA+TGG 0.576328 2.3:+34985031 MS.gene066732:CDS
CATAAACAATTGTTTGTCTG+TGG 0.584673 2.3:-34985276 None:intergenic
AAAGAGTTGTCAACGGAAAG+CGG 0.589356 2.3:+34985800 MS.gene066732:CDS
AAAATTTGTGTGCTCCAAAG+AGG 0.589431 2.3:+34984664 MS.gene066732:CDS
TACCGGCTTGTACAAGCATA+GGG 0.592516 2.3:-34985888 None:intergenic
GAATTAGTGTCAAAACAAGC+TGG 0.597771 2.3:+34984950 MS.gene066732:CDS
GTCTTACCGGTGTCCATAGG+TGG 0.607232 2.3:-34984279 None:intergenic
CCTTTCTCAAAGCAATGTGT+GGG 0.608035 2.3:+34985353 MS.gene066732:CDS
CACTTTGAAAGAGTTGTCAA+CGG 0.610932 2.3:+34985793 MS.gene066732:CDS
AACGTTGGACAAGAGATGCA+AGG 0.614203 2.3:+34986175 MS.gene066732:CDS
GAATATTACATCCAATCCAA+TGG 0.614280 2.3:+34984439 MS.gene066732:CDS
AAATTTGTGTGCTCCAAAGA+GGG 0.614397 2.3:+34984665 MS.gene066732:CDS
CTTCACCATACCTTAGTGAG+TGG 0.628280 2.3:-34985036 None:intergenic
TTAGTGTCAAAACAAGCTGG+TGG 0.629473 2.3:+34984953 MS.gene066732:CDS
ACATAAGGGAAAATGCCCTA+AGG 0.636592 2.3:+34985473 MS.gene066732:CDS
TACAAATTATGATAACAGCA+AGG 0.637062 2.3:+34986008 MS.gene066732:CDS
GCTGGTGGAATAAACACTGT+TGG 0.638577 2.3:+34984968 MS.gene066732:CDS
ATGTAATCAAAATTGGAAAG+GGG 0.639132 2.3:+34986203 MS.gene066732:CDS
TCAAACTCCATTTCCAAGTG+TGG 0.642402 2.3:-34984530 None:intergenic
GGACATTGTTGATCTTGGTG+GGG 0.645736 2.3:+34986470 MS.gene066732:CDS
AGGCCAACAAATACTCCCAA+CGG 0.650363 2.3:-34985247 None:intergenic
GTGTATTGATCTACACGAAG+AGG 0.652482 2.3:+34985549 MS.gene066732:CDS
TGCTCCAAAGAGGGTAGACG+CGG 0.660484 2.3:+34984674 MS.gene066732:CDS
GTGAATGATATCGTGCAACA+GGG 0.667513 2.3:+34984482 MS.gene066732:CDS
TCATCACCACCTATGGACAC+CGG 0.690966 2.3:+34984273 MS.gene066732:CDS
ATTATGATAACAGCAAGGAG+CGG 0.694152 2.3:+34986013 MS.gene066732:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTATAATGAGTATAGT+AGG + chr2.3:34984573-34984592 MS.gene066732:CDS 15.0%
!!! ATGAATATTTTGAAAGTATT+CGG + chr2.3:34984337-34984356 MS.gene066732:intron 15.0%
!! AACAATATATACTAAAACGT+TGG + chr2.3:34986160-34986179 MS.gene066732:CDS 20.0%
!! ATCAATTGCAAATGAAAAAA+AGG + chr2.3:34986437-34986456 MS.gene066732:CDS 20.0%
!! ATTATAAAATTCATATGCCA+AGG - chr2.3:34984564-34984583 None:intergenic 20.0%
!! CACAAATGATAAATGATTAT+GGG + chr2.3:34985167-34985186 MS.gene066732:CDS 20.0%
!! GTTTAAGAAATTTGAAAGAA+GGG + chr2.3:34985974-34985993 MS.gene066732:CDS 20.0%
!! TATTCACATTATCTAGAAAA+TGG - chr2.3:34984424-34984443 None:intergenic 20.0%
!! TGTTTAAGAAATTTGAAAGA+AGG + chr2.3:34985973-34985992 MS.gene066732:CDS 20.0%
!!! ACTAATTCATGAAAATCTTT+TGG - chr2.3:34984938-34984957 None:intergenic 20.0%
!!! AGCAAATAACAAACATATTT+TGG + chr2.3:34985137-34985156 MS.gene066732:CDS 20.0%
!!! ATCTTTTTCATTTTCAATCT+TGG - chr2.3:34985868-34985887 None:intergenic 20.0%
!!! GCAAATAACAAACATATTTT+GGG + chr2.3:34985138-34985157 MS.gene066732:CDS 20.0%
! ACAGACAAACAATTGTTTAT+GGG + chr2.3:34985278-34985297 MS.gene066732:CDS 25.0%
! ATAAATGATTATGGGTACTT+TGG + chr2.3:34985175-34985194 MS.gene066732:CDS 25.0%
! ATCATTTATCATTTGTGCAT+CGG - chr2.3:34985164-34985183 None:intergenic 25.0%
! ATGTAATCAAAATTGGAAAG+GGG + chr2.3:34986203-34986222 MS.gene066732:CDS 25.0%
! CAAATAACCATTGAAAAGAA+TGG - chr2.3:34985330-34985349 None:intergenic 25.0%
! CCTTGAATCGAAAAATATTA+TGG - chr2.3:34985642-34985661 None:intergenic 25.0%
! GACTAAGAGAAAGAAAAAAT+TGG + chr2.3:34986593-34986612 MS.gene066732:CDS 25.0%
! GATGTAATCAAAATTGGAAA+GGG + chr2.3:34986202-34986221 MS.gene066732:CDS 25.0%
! GCAAATAAAAGCAAAAAAGA+TGG + chr2.3:34984626-34984645 MS.gene066732:CDS 25.0%
! GCACAAATGATAAATGATTA+TGG + chr2.3:34985166-34985185 MS.gene066732:CDS 25.0%
! TACAAATTATGATAACAGCA+AGG + chr2.3:34986008-34986027 MS.gene066732:CDS 25.0%
! TGAAACTCATAAAAGAATGA+AGG - chr2.3:34985103-34985122 None:intergenic 25.0%
!! AAACAATCCATTCTTTTCAA+TGG + chr2.3:34985320-34985339 MS.gene066732:CDS 25.0%
!! ATAATGTTTCTAAAGACTCA+TGG + chr2.3:34985614-34985633 MS.gene066732:CDS 25.0%
!! ATTCTTATTTGTCTTGAATG+TGG - chr2.3:34985221-34985240 None:intergenic 25.0%
!! ATTCTTTTATGAGTTTCAGA+TGG + chr2.3:34985105-34985124 MS.gene066732:CDS 25.0%
!! TTTTCGATTCAAGGAAAAAT+GGG + chr2.3:34985648-34985667 MS.gene066732:CDS 25.0%
!!! CCATAATATTTTTCGATTCA+AGG + chr2.3:34985639-34985658 MS.gene066732:CDS 25.0%
!!! CGTTTTAGTATATATTGTTG+AGG - chr2.3:34986159-34986178 None:intergenic 25.0%
!!! TTTTATTTGCATATTCTCGA+CGG - chr2.3:34984616-34984635 None:intergenic 25.0%
!!! TTTTTCGATTCAAGGAAAAA+TGG + chr2.3:34985647-34985666 MS.gene066732:CDS 25.0%
AAATAAGAATTATCGTCCGT+TGG + chr2.3:34985231-34985250 MS.gene066732:CDS 30.0%
AATAAGAATTATCGTCCGTT+GGG + chr2.3:34985232-34985251 MS.gene066732:CDS 30.0%
AATGAGTATAGTAGGAAATG+TGG + chr2.3:34984581-34984600 MS.gene066732:CDS 30.0%
ACGTGATTACTTAACAAAAG+AGG + chr2.3:34984706-34984725 MS.gene066732:CDS 30.0%
ATCATATAAACATACTCAGC+TGG - chr2.3:34984845-34984864 None:intergenic 30.0%
ATGAGACTCTTCATAAACTT+TGG - chr2.3:34986346-34986365 None:intergenic 30.0%
ATTCATCAGCCAATACAATT+TGG - chr2.3:34984904-34984923 None:intergenic 30.0%
CACAGACAAACAATTGTTTA+TGG + chr2.3:34985277-34985296 MS.gene066732:CDS 30.0%
CAGACAAACAATTGTTTATG+GGG + chr2.3:34985279-34985298 MS.gene066732:CDS 30.0%
CATAAACAATTGTTTGTCTG+TGG - chr2.3:34985279-34985298 None:intergenic 30.0%
GAATATTACATCCAATCCAA+TGG + chr2.3:34984439-34984458 MS.gene066732:CDS 30.0%
GCTTAGATAGGACTAAAAAA+AGG + chr2.3:34986517-34986536 MS.gene066732:CDS 30.0%
GGATGTAATCAAAATTGGAA+AGG + chr2.3:34986201-34986220 MS.gene066732:CDS 30.0%
GGTTAGGATGTAATCAAAAT+TGG + chr2.3:34986196-34986215 MS.gene066732:CDS 30.0%
TGAAAAAAAGGATCAGAATG+TGG + chr2.3:34986449-34986468 MS.gene066732:CDS 30.0%
TGGAATTCCTCAAAAATTCT+TGG - chr2.3:34985925-34985944 None:intergenic 30.0%
TGGATAAAAAGAGACAGAAA+AGG + chr2.3:34986562-34986581 MS.gene066732:CDS 30.0%
TTCATCAGCCAATACAATTT+GGG - chr2.3:34984903-34984922 None:intergenic 30.0%
! GTATGGTTATTTCACTTGAT+CGG + chr2.3:34984762-34984781 MS.gene066732:CDS 30.0%
! TATAGTAGGAAATGTGGTTT+TGG + chr2.3:34984587-34984606 MS.gene066732:CDS 30.0%
! TATGCTGACTAAAAAGTCTA+TGG + chr2.3:34986380-34986399 MS.gene066732:CDS 30.0%
! TGATCTGTTAGAATGGTTTT+AGG - chr2.3:34985385-34985404 None:intergenic 30.0%
! TTACACTCCAAGAATTTTTG+AGG + chr2.3:34985915-34985934 MS.gene066732:CDS 30.0%
!!! AAGAAGAATTTTTGAGTGGA+TGG + chr2.3:34985572-34985591 MS.gene066732:CDS 30.0%
!!! AGAAGAATTTTTGAGTGGAT+GGG + chr2.3:34985573-34985592 MS.gene066732:CDS 30.0%
AAAATTTGTGTGCTCCAAAG+AGG + chr2.3:34984664-34984683 MS.gene066732:CDS 35.0%
AAATTTGTGTGCTCCAAAGA+GGG + chr2.3:34984665-34984684 MS.gene066732:CDS 35.0%
AATGTGGACATTGTTGATCT+TGG + chr2.3:34986465-34986484 MS.gene066732:CDS 35.0%
ACAAGCATCCCAAATTGTAT+TGG + chr2.3:34984892-34984911 MS.gene066732:CDS 35.0%
ATAGATGATTCACATGCCTT+AGG - chr2.3:34985492-34985511 None:intergenic 35.0%
ATTATGATAACAGCAAGGAG+CGG + chr2.3:34986013-34986032 MS.gene066732:CDS 35.0%
ATTCAAGGAAAAATGGGCAT+GGG + chr2.3:34985654-34985673 MS.gene066732:CDS 35.0%
ATTTCACTTGATCGGAAGAT+TGG + chr2.3:34984770-34984789 MS.gene066732:CDS 35.0%
CACAATCCATGAAATGTTTC+TGG - chr2.3:34985445-34985464 None:intergenic 35.0%
CTGACTAAAAAGTCTATGGA+TGG + chr2.3:34986384-34986403 MS.gene066732:CDS 35.0%
CTTCTGTCTTGACTCATATT+CGG - chr2.3:34985842-34985861 None:intergenic 35.0%
GAAAGGGGAACATATTGAAT+TGG + chr2.3:34986218-34986237 MS.gene066732:CDS 35.0%
GAATTAGTGTCAAAACAAGC+TGG + chr2.3:34984950-34984969 MS.gene066732:CDS 35.0%
GACAAAGAACAAAGTCTTAC+CGG - chr2.3:34984295-34984314 None:intergenic 35.0%
GACATTCCTCATATTCATCT+TGG - chr2.3:34985945-34985964 None:intergenic 35.0%
GATAGTAGTAGGGAATCATA+TGG + chr2.3:34986035-34986054 MS.gene066732:CDS 35.0%
GGAATTCCAAGATGAATATG+AGG + chr2.3:34985936-34985955 MS.gene066732:CDS 35.0%
TAATGTACCACACTTGGAAA+TGG + chr2.3:34984523-34984542 MS.gene066732:CDS 35.0%
TAGATGATTCACATGCCTTA+GGG - chr2.3:34985491-34985510 None:intergenic 35.0%
TAGTCCTATCTAAGCAATCT+CGG - chr2.3:34986512-34986531 None:intergenic 35.0%
TAGTGCGAAGCAAATTTCTA+TGG - chr2.3:34985009-34985028 None:intergenic 35.0%
TCGGAAGATTGGAAAATACA+AGG + chr2.3:34984781-34984800 MS.gene066732:CDS 35.0%
TCTTGGAGTGTAAACATTAC+CGG - chr2.3:34985908-34985927 None:intergenic 35.0%
TGCATCTTGATCTGTTAGAA+TGG - chr2.3:34985392-34985411 None:intergenic 35.0%
TGTTAGTAATGTACCACACT+TGG + chr2.3:34984517-34984536 MS.gene066732:CDS 35.0%
TTGTTTGTCTGTGGCTATTA+AGG - chr2.3:34985270-34985289 None:intergenic 35.0%
! CACTTTGAAAGAGTTGTCAA+CGG + chr2.3:34985793-34985812 MS.gene066732:CDS 35.0%
! GAAATTTGCTTCGCACTAAA+AGG + chr2.3:34985011-34985030 MS.gene066732:CDS 35.0%
! GGTTGAAGGTGTAGAAAAAT+TGG + chr2.3:34984460-34984479 MS.gene066732:CDS 35.0%
! TGTCGAAAATTTGAGACACA+TGG + chr2.3:34986078-34986097 MS.gene066732:CDS 35.0%
! TTTTCTACACCTTCAACCAT+TGG - chr2.3:34984458-34984477 None:intergenic 35.0%
!! ATTGTATTGGCTGATGAATC+TGG + chr2.3:34984905-34984924 MS.gene066732:CDS 35.0%
!! CTAAAAAAAGGCATCCAACT+TGG + chr2.3:34986529-34986548 MS.gene066732:CDS 35.0%
!! GAGGAAGAAGAATTTTTGAG+TGG + chr2.3:34985568-34985587 MS.gene066732:CDS 35.0%
AAAGAGTTGTCAACGGAAAG+CGG + chr2.3:34985800-34985819 MS.gene066732:CDS 40.0%
AACAGATCAAGATGCAGCAA+TGG + chr2.3:34985396-34985415 MS.gene066732:CDS 40.0%
AACAGGATGTTTAGCACGTA+TGG + chr2.3:34984745-34984764 MS.gene066732:CDS 40.0%
ACATAAGGGAAAATGCCCTA+AGG + chr2.3:34985473-34985492 MS.gene066732:CDS 40.0%
ACATCCAATCCAATGGTTGA+AGG + chr2.3:34984446-34984465 MS.gene066732:CDS 40.0%
ACCTTTCTCAAAGCAATGTG+TGG + chr2.3:34985352-34985371 MS.gene066732:CDS 40.0%
ATTGTGCACTTGGCACATAA+GGG + chr2.3:34985459-34985478 MS.gene066732:CDS 40.0%
CCTTTCTCAAAGCAATGTGT+GGG + chr2.3:34985353-34985372 MS.gene066732:CDS 40.0%
CTAAAAGGAACCACTCACTA+AGG + chr2.3:34985026-34985045 MS.gene066732:CDS 40.0%
GATTCAAGGAAAAATGGGCA+TGG + chr2.3:34985653-34985672 MS.gene066732:CDS 40.0%
GTCATGCCAGAAACATTTCA+TGG + chr2.3:34985436-34985455 MS.gene066732:CDS 40.0%
GTGAATGATATCGTGCAACA+GGG + chr2.3:34984482-34984501 MS.gene066732:CDS 40.0%
TACACCTTCAACCATTGGAT+TGG - chr2.3:34984453-34984472 None:intergenic 40.0%
TCAAACTCCATTTCCAAGTG+TGG - chr2.3:34984533-34984552 None:intergenic 40.0%
TCACTAAGGTATGGTGAAGT+AGG + chr2.3:34985040-34985059 MS.gene066732:CDS 40.0%
TCCAATTTCTCAAGCCAAGT+TGG - chr2.3:34986546-34986565 None:intergenic 40.0%
TCTATGGATGGAGAGTCAAT+TGG + chr2.3:34986396-34986415 MS.gene066732:CDS 40.0%
TGGACATTGTTGATCTTGGT+GGG + chr2.3:34986469-34986488 MS.gene066732:CDS 40.0%
TTAGTGTCAAAACAAGCTGG+TGG + chr2.3:34984953-34984972 MS.gene066732:CDS 40.0%
TTGCTCAATTTAACTGCTCG+AGG - chr2.3:34986285-34986304 None:intergenic 40.0%
! CATTTCATGGATTGTGCACT+TGG + chr2.3:34985449-34985468 MS.gene066732:CDS 40.0%
! GTGTATTGATCTACACGAAG+AGG + chr2.3:34985549-34985568 MS.gene066732:CDS 40.0%
! TCCAACTTGGCTTGAGAAAT+TGG + chr2.3:34986542-34986561 MS.gene066732:CDS 40.0%
!!! AGAATTTTTGAGTGGATGGG+AGG + chr2.3:34985576-34985595 MS.gene066732:CDS 40.0%
AAAGTCTTACCGGTGTCCAT+AGG - chr2.3:34984285-34984304 None:intergenic 45.0%
AACGTTGGACAAGAGATGCA+AGG + chr2.3:34986175-34986194 MS.gene066732:CDS 45.0%
AGAGCCGAGATTGCTTAGAT+AGG + chr2.3:34986505-34986524 MS.gene066732:CDS 45.0%
AGGAACCACTCACTAAGGTA+TGG + chr2.3:34985031-34985050 MS.gene066732:CDS 45.0%
AGGCCAACAAATACTCCCAA+CGG - chr2.3:34985250-34985269 None:intergenic 45.0%
ATCCCTATGCTTGTACAAGC+CGG + chr2.3:34985886-34985905 MS.gene066732:CDS 45.0%
CTTCACCATACCTTAGTGAG+TGG - chr2.3:34985039-34985058 None:intergenic 45.0%
GATTGTGCACTTGGCACATA+AGG + chr2.3:34985458-34985477 MS.gene066732:CDS 45.0%
GCTGGTGGAATAAACACTGT+TGG + chr2.3:34984968-34984987 MS.gene066732:CDS 45.0%
GGAAATGGAGTTTGAGTCTG+AGG + chr2.3:34984538-34984557 MS.gene066732:CDS 45.0%
GGACATTGTTGATCTTGGTG+GGG + chr2.3:34986470-34986489 MS.gene066732:CDS 45.0%
GGTGAATGATATCGTGCAAC+AGG + chr2.3:34984481-34984500 MS.gene066732:CDS 45.0%
GTGGACATTGTTGATCTTGG+TGG + chr2.3:34986468-34986487 MS.gene066732:CDS 45.0%
TCAAGATGCAGCAATGGCTA+AGG + chr2.3:34985402-34985421 MS.gene066732:CDS 45.0%
TCACCATTCATCACCACCTA+TGG + chr2.3:34984266-34984285 MS.gene066732:CDS 45.0%
TGGACAAGAGATGCAAGGTT+AGG + chr2.3:34986180-34986199 MS.gene066732:CDS 45.0%
! TGTCCATAGGTGGTGATGAA+TGG - chr2.3:34984272-34984291 None:intergenic 45.0%
!! CCCACACATTGCTTTGAGAA+AGG - chr2.3:34985356-34985375 None:intergenic 45.0%
!! TACCGGCTTGTACAAGCATA+GGG - chr2.3:34985891-34985910 None:intergenic 45.0%
!! TTACCGGCTTGTACAAGCAT+AGG - chr2.3:34985892-34985911 None:intergenic 45.0%
AGCAAGGAGCGGATAGTAGT+AGG + chr2.3:34986024-34986043 MS.gene066732:CDS 50.0%
CATACTCAGCTGGCAGAAGA+AGG - chr2.3:34984835-34984854 None:intergenic 50.0%
GCAAGGAGCGGATAGTAGTA+GGG + chr2.3:34986025-34986044 MS.gene066732:CDS 50.0%
GCTCGAGCAGAAACTAGAAC+AGG + chr2.3:34984728-34984747 MS.gene066732:CDS 50.0%
TCATCACCACCTATGGACAC+CGG + chr2.3:34984273-34984292 MS.gene066732:CDS 50.0%
!! CGTCCGTTGGGAGTATTTGT+TGG + chr2.3:34985244-34985263 MS.gene066732:CDS 50.0%
AACACCGCGTCTACCCTCTT+TGG - chr2.3:34984681-34984700 None:intergenic 55.0%
GTCTTACCGGTGTCCATAGG+TGG - chr2.3:34984282-34984301 None:intergenic 55.0%
TGCTCCAAAGAGGGTAGACG+CGG + chr2.3:34984674-34984693 MS.gene066732:CDS 55.0%
! GGAGTTTGAGTCTGAGGCCT+TGG + chr2.3:34984544-34984563 MS.gene066732:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 34984228 34986629 34984228 ID=MS.gene066732
chr2.3 mRNA 34984228 34986629 34984228 ID=MS.gene066732.t1;Parent=MS.gene066732
chr2.3 exon 34984228 34984294 34984228 ID=MS.gene066732.t1.exon1;Parent=MS.gene066732.t1
chr2.3 CDS 34984228 34984294 34984228 ID=cds.MS.gene066732.t1;Parent=MS.gene066732.t1
chr2.3 exon 34984432 34986629 34984432 ID=MS.gene066732.t1.exon2;Parent=MS.gene066732.t1
chr2.3 CDS 34984432 34986629 34984432 ID=cds.MS.gene066732.t1;Parent=MS.gene066732.t1
Gene Sequence

>MS.gene066732

ATGGTAGAAACGACACACGTAGAACATGATGCAAATCTTCACCATTCATCACCACCTATGGACACCGGTAAGACTTTGTTCTTTGTCGATGAATATACACACGTAGAACATGAATATTTTGAAAGTATTCGGACAATATAAAATTAATATAAAATGTCTATGAATATTAACATGTTGATTTTATATTTTTTTTCCATTTTCTAGATAATGTGAATATTACATCCAATCCAATGGTTGAAGGTGTAGAAAAATTGGTGAATGATATCGTGCAACAGGGAAAAGAGAGTACTGTTAGTAATGTACCACACTTGGAAATGGAGTTTGAGTCTGAGGCCTTGGCATATGAATTTTATAATGAGTATAGTAGGAAATGTGGTTTTGGTATCCGTCGAGAATATGCAAATAAAAGCAAAAAAGATGGAGTTCTTACTTCAAGAAAATTTGTGTGCTCCAAAGAGGGTAGACGCGGTGTTGACAAACGTGATTACTTAACAAAAGAGGCTCGAGCAGAAACTAGAACAGGATGTTTAGCACGTATGGTTATTTCACTTGATCGGAAGATTGGAAAATACAAGGTTATTGACTTTGTAGCTGAACATAATCACCTTCTTCTGCCAGCTGAGTATGTTTATATGATTCGCTCTCATCGACACATATCTGAGTCACAAGCATCCCAAATTGTATTGGCTGATGAATCTGGATTGAAACCAAAAGATTTTCATGAATTAGTGTCAAAACAAGCTGGTGGAATAAACACTGTTGGTTATACAAGACAAGACCATAGAAATTTGCTTCGCACTAAAAGGAACCACTCACTAAGGTATGGTGAAGTAGGTGCGTTACTAATACATTTCAAAAAGCAAAGTGAAAATCCTTCATTCTTTTATGAGTTTCAGATGGATGTTGAAGAGCAAATAACAAACATATTTTGGGCCGATGCACAAATGATAAATGATTATGGGTACTTTGGTGATGTTGTCACTTTTGACACCACATTCAAGACAAATAAGAATTATCGTCCGTTGGGAGTATTTGTTGGCCTTAATAGCCACAGACAAACAATTGTTTATGGGGCAGCATTGTTATATGATGAAACAATCCATTCTTTTCAATGGTTATTTGAAACCTTTCTCAAAGCAATGTGTGGGAAAAAGCCTAAAACCATTCTAACAGATCAAGATGCAGCAATGGCTAAGGCTCTTTCTATAGTCATGCCAGAAACATTTCATGGATTGTGCACTTGGCACATAAGGGAAAATGCCCTAAGGCATGTGAATCATCTATATCAACAGAGCTCACAATTCTGCTCAGATTTTGAAGCGTGTATTGATCTACACGAAGAGGAAGAAGAATTTTTGAGTGGATGGGAGGCTTTACTAGTTGAGCATAATGTTTCTAAAGACTCATGGCTCCATAATATTTTTCGATTCAAGGAAAAATGGGCATGGGCTTATGTGCGAAAGACTTTCACTGCAGATATGAGATCTACTCAATTAAGTGAGAGTTTTAATGCTGATTTAAAAAATCATTTGAAGTCAGATTTAAATTTGATACAATTTTTCACACACTTTGAAAGAGTTGTCAACGGAAAGCGGAATAATGAGTCAGAAGCCGAATATGAGTCAAGACAGAAGTTGCCAAGATTGAAAATGAAAAAGATCCCTATGCTTGTACAAGCCGGTAATGTTTACACTCCAAGAATTTTTGAGGAATTCCAAGATGAATATGAGGAATGTCAAGCTTCTTGTTTAAGAAATTTGAAAGAAGGGTCATATGTTGTTACAAATTATGATAACAGCAAGGAGCGGATAGTAGTAGGGAATCATATGGATAAAACAGTTGCTTGTGATTGTCGAAAATTTGAGACACATGGAATCTTATGCAGTCATGCTTTGAAAGTTTTAGATTTTATGAATGTTAAGCTAATTCCTCAACAATATATACTAAAACGTTGGACAAGAGATGCAAGGTTAGGATGTAATCAAAATTGGAAAGGGGAACATATTGAATTGGACATAAAAGCTCATTTCATGAAACGATACAATGAGTTATGTCCTCGAGCAGTTAAATTGAGCAATAGAGCATCAGAATCTCATGAAACTTACACATTTTTGTCCAAAGTTTATGAAGAGTCTCATAAAATAATTGAAGATATGCTGACTAAAAAGTCTATGGATGGAGAGTCAATTGGAATGAGTCATGTGTCTATATCAATTGCAAATGAAAAAAAGGATCAGAATGTGGACATTGTTGATCTTGGTGGGGCAAAAGCAATAAAGAGCCGAGATTGCTTAGATAGGACTAAAAAAAGGCATCCAACTTGGCTTGAGAAATTGGATAAAAAGAGACAGAAAAGGCTATCACAGACTAAGAGAAAGAAAAAATTGGTAAACAACTCTTAA
Protein sequence

>MS.gene066732.t1

MVETTHVEHDANLHHSSPPMDTDNVNITSNPMVEGVEKLVNDIVQQGKESTVSNVPHLEMEFESEALAYEFYNEYSRKCGFGIRREYANKSKKDGVLTSRKFVCSKEGRRGVDKRDYLTKEARAETRTGCLARMVISLDRKIGKYKVIDFVAEHNHLLLPAEYVYMIRSHRHISESQASQIVLADESGLKPKDFHELVSKQAGGINTVGYTRQDHRNLLRTKRNHSLRYGEVGALLIHFKKQSENPSFFYEFQMDVEEQITNIFWADAQMINDYGYFGDVVTFDTTFKTNKNYRPLGVFVGLNSHRQTIVYGAALLYDETIHSFQWLFETFLKAMCGKKPKTILTDQDAAMAKALSIVMPETFHGLCTWHIRENALRHVNHLYQQSSQFCSDFEACIDLHEEEEEFLSGWEALLVEHNVSKDSWLHNIFRFKEKWAWAYVRKTFTADMRSTQLSESFNADLKNHLKSDLNLIQFFTHFERVVNGKRNNESEAEYESRQKLPRLKMKKIPMLVQAGNVYTPRIFEEFQDEYEECQASCLRNLKEGSYVVTNYDNSKERIVVGNHMDKTVACDCRKFETHGILCSHALKVLDFMNVKLIPQQYILKRWTRDARLGCNQNWKGEHIELDIKAHFMKRYNELCPRAVKLSNRASESHETYTFLSKVYEESHKIIEDMLTKKSMDGESIGMSHVSISIANEKKDQNVDIVDLGGAKAIKSRDCLDRTKKRHPTWLEKLDKKRQKRLSQTKRKKKLVNNS