Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06678.t1 | RHN49639.1 | 73.5 | 162 | 27 | 2 | 3 | 149 | 7 | 167 | 1.20E-57 | 232.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06678.t1 | A0A396H8N5 | 73.5 | 162 | 27 | 2 | 3 | 149 | 7 | 167 | 8.3e-58 | 232.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene06678.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06678.t1 | MTR_7g117590 | 71.605 | 162 | 31 | 2 | 2 | 149 | 12 | 172 | 7.17e-78 | 228 |
| MS.gene06678.t1 | MTR_1g054470 | 52.083 | 144 | 43 | 2 | 2 | 145 | 23 | 140 | 9.25e-45 | 144 |
| MS.gene06678.t1 | MTR_2g012700 | 37.273 | 110 | 68 | 1 | 40 | 148 | 10 | 119 | 1.84e-27 | 99.4 |
| MS.gene06678.t1 | MTR_2g062330 | 39.655 | 116 | 65 | 3 | 34 | 148 | 27 | 138 | 3.60e-26 | 96.7 |
| MS.gene06678.t1 | MTR_5g071400 | 62.500 | 56 | 20 | 1 | 3 | 58 | 9 | 63 | 9.91e-19 | 76.3 |
| MS.gene06678.t1 | MTR_6g477950 | 38.095 | 105 | 55 | 3 | 48 | 148 | 46 | 144 | 3.24e-15 | 71.2 |
| MS.gene06678.t1 | MTR_0010s0310 | 36.471 | 85 | 51 | 1 | 58 | 139 | 156 | 240 | 1.92e-14 | 68.6 |
| MS.gene06678.t1 | MTR_5g071400 | 65.854 | 41 | 13 | 1 | 18 | 58 | 1 | 40 | 2.21e-12 | 58.9 |
| MS.gene06678.t1 | MTR_5g078270 | 34.118 | 85 | 53 | 1 | 58 | 139 | 214 | 298 | 2.59e-12 | 63.2 |
| MS.gene06678.t1 | MTR_7g117585 | 36.905 | 84 | 50 | 2 | 68 | 149 | 64 | 146 | 4.72e-12 | 60.1 |
| MS.gene06678.t1 | MTR_6g087720 | 33.333 | 87 | 57 | 1 | 51 | 136 | 212 | 298 | 8.51e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCATCAACTATTCGAATTT+TGG | 0.090848 | 3.3:-76633441 | None:intergenic |
| TACAGGTAATGGGTGAAAAT+TGG | 0.209416 | 3.3:-76633153 | None:intergenic |
| GTGGATGGTTTGAGTTTGTT+AGG | 0.256057 | 3.3:+76633531 | MS.gene06678:CDS |
| CCATCGACTTCTTCTTCGTT+TGG | 0.266681 | 3.3:-76633185 | None:intergenic |
| GGCTGTTGGACGGAGTGTTC+TGG | 0.356206 | 3.3:+76633283 | MS.gene06678:CDS |
| CGAATAGTTGATGATGAAAC+TGG | 0.373610 | 3.3:+76633449 | MS.gene06678:CDS |
| GAATGTGAAGTTCGTGATAA+AGG | 0.381874 | 3.3:+76633482 | MS.gene06678:CDS |
| AAGTTCGTGATAAAGGAAAT+TGG | 0.388847 | 3.3:+76633489 | MS.gene06678:CDS |
| ACAGGTAATGGGTGAAAATT+GGG | 0.407202 | 3.3:-76633152 | None:intergenic |
| ATTAGTTCTGCTACGGCTGT+TGG | 0.467637 | 3.3:+76633269 | MS.gene06678:CDS |
| TGGATGGTTTGAGTTTGTTA+GGG | 0.477462 | 3.3:+76633532 | MS.gene06678:CDS |
| AAATGTCTTCTTCTAGTAGC+AGG | 0.479507 | 3.3:+76633022 | None:intergenic |
| TTTGAAATTGTGCAGATACT+GGG | 0.501474 | 3.3:-76633208 | None:intergenic |
| CTGCAGCCATGTAATTGATC+CGG | 0.509939 | 3.3:+76633112 | MS.gene06678:intron |
| ATGGAGAAATACGTTGGTAG+TGG | 0.538951 | 3.3:+76633512 | MS.gene06678:CDS |
| CCATGTAATTGATCCGGAGT+TGG | 0.545499 | 3.3:+76633118 | MS.gene06678:CDS |
| GGCATCAATTCGTCCAACTC+CGG | 0.549037 | 3.3:-76633131 | None:intergenic |
| CCAACTCCGGATCAATTACA+TGG | 0.550497 | 3.3:-76633118 | None:intergenic |
| AGAAATACGTTGGTAGTGGA+TGG | 0.551017 | 3.3:+76633516 | MS.gene06678:CDS |
| CTTTGAATTGCAATCTTCCG+AGG | 0.557056 | 3.3:-76633401 | None:intergenic |
| TCGTGATAAAGGAAATTGGA+TGG | 0.565929 | 3.3:+76633493 | MS.gene06678:CDS |
| GCATAGTATTAGTTCTGCTA+CGG | 0.571784 | 3.3:+76633262 | MS.gene06678:CDS |
| TGAATGTGTAGCATATTCCT+CGG | 0.575111 | 3.3:+76633384 | MS.gene06678:intron |
| AGGGCAAAAGAACTCAAAGA+AGG | 0.584629 | 3.3:+76633551 | MS.gene06678:CDS |
| GATACAAATAATTTGTGTGG+AGG | 0.593345 | 3.3:-76633602 | None:intergenic |
| GTTCTGCTACGGCTGTTGGA+CGG | 0.598239 | 3.3:+76633273 | MS.gene06678:CDS |
| AATTGGATGGAGAAATACGT+TGG | 0.604235 | 3.3:+76633506 | MS.gene06678:CDS |
| ACTGATACAAATAATTTGTG+TGG | 0.607437 | 3.3:-76633605 | None:intergenic |
| CCAAACGAAGAAGAAGTCGA+TGG | 0.670927 | 3.3:+76633185 | MS.gene06678:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTTTAATTTTATTTTTGC+TGG | + | chr3.3:76633308-76633327 | MS.gene06678:intron | 15.0% |
| !!! | CAATTTCAAAATTTTCAAAA+CGG | + | chr3.3:76633221-76633240 | MS.gene06678:CDS | 15.0% |
| !!! | TTTTTGTTTTTTTAATCTTC+TGG | + | chr3.3:76633066-76633085 | MS.gene06678:intron | 15.0% |
| !! | ACTGATACAAATAATTTGTG+TGG | - | chr3.3:76633608-76633627 | None:intergenic | 25.0% |
| !! | ATCATCAACTATTCGAATTT+TGG | - | chr3.3:76633444-76633463 | None:intergenic | 25.0% |
| AAGTTCGTGATAAAGGAAAT+TGG | + | chr3.3:76633489-76633508 | MS.gene06678:CDS | 30.0% | |
| ! | GATACAAATAATTTGTGTGG+AGG | - | chr3.3:76633605-76633624 | None:intergenic | 30.0% |
| ! | TTTGAAATTGTGCAGATACT+GGG | - | chr3.3:76633211-76633230 | None:intergenic | 30.0% |
| !!! | TTTTGAAATTGTGCAGATAC+TGG | - | chr3.3:76633212-76633231 | None:intergenic | 30.0% |
| AATTGGATGGAGAAATACGT+TGG | + | chr3.3:76633506-76633525 | MS.gene06678:CDS | 35.0% | |
| GAATGTGAAGTTCGTGATAA+AGG | + | chr3.3:76633482-76633501 | MS.gene06678:CDS | 35.0% | |
| TCGTGATAAAGGAAATTGGA+TGG | + | chr3.3:76633493-76633512 | MS.gene06678:CDS | 35.0% | |
| TGAATGTGTAGCATATTCCT+CGG | + | chr3.3:76633384-76633403 | MS.gene06678:intron | 35.0% | |
| ! | GATTTTCGTCTACAGGTAAT+GGG | - | chr3.3:76633166-76633185 | None:intergenic | 35.0% |
| !! | ACAGGTAATGGGTGAAAATT+GGG | - | chr3.3:76633155-76633174 | None:intergenic | 35.0% |
| !! | CGAATAGTTGATGATGAAAC+TGG | + | chr3.3:76633449-76633468 | MS.gene06678:CDS | 35.0% |
| !! | GCATAGTATTAGTTCTGCTA+CGG | + | chr3.3:76633262-76633281 | MS.gene06678:CDS | 35.0% |
| !! | TACAGGTAATGGGTGAAAAT+TGG | - | chr3.3:76633156-76633175 | None:intergenic | 35.0% |
| !! | TGGATGGTTTGAGTTTGTTA+GGG | + | chr3.3:76633532-76633551 | MS.gene06678:CDS | 35.0% |
| AGGGCAAAAGAACTCAAAGA+AGG | + | chr3.3:76633551-76633570 | MS.gene06678:CDS | 40.0% | |
| CTTTGAATTGCAATCTTCCG+AGG | - | chr3.3:76633404-76633423 | None:intergenic | 40.0% | |
| TTGCTGGATTATTTGCGCAT+GGG | + | chr3.3:76633324-76633343 | MS.gene06678:intron | 40.0% | |
| TTTGCTGGATTATTTGCGCA+TGG | + | chr3.3:76633323-76633342 | MS.gene06678:intron | 40.0% | |
| ! | ATGGAGAAATACGTTGGTAG+TGG | + | chr3.3:76633512-76633531 | MS.gene06678:CDS | 40.0% |
| ! | GGATTTTCGTCTACAGGTAA+TGG | - | chr3.3:76633167-76633186 | None:intergenic | 40.0% |
| ! | GTGGATGGTTTGAGTTTGTT+AGG | + | chr3.3:76633531-76633550 | MS.gene06678:CDS | 40.0% |
| ! | TCGTTTGGATTTTCGTCTAC+AGG | - | chr3.3:76633173-76633192 | None:intergenic | 40.0% |
| !! | AGAAATACGTTGGTAGTGGA+TGG | + | chr3.3:76633516-76633535 | MS.gene06678:CDS | 40.0% |
| CCAAACGAAGAAGAAGTCGA+TGG | + | chr3.3:76633185-76633204 | MS.gene06678:CDS | 45.0% | |
| CCAACTCCGGATCAATTACA+TGG | - | chr3.3:76633121-76633140 | None:intergenic | 45.0% | |
| CCATGTAATTGATCCGGAGT+TGG | + | chr3.3:76633118-76633137 | MS.gene06678:CDS | 45.0% | |
| CTGCAGCCATGTAATTGATC+CGG | + | chr3.3:76633112-76633131 | MS.gene06678:intron | 45.0% | |
| !! | ATTAGTTCTGCTACGGCTGT+TGG | + | chr3.3:76633269-76633288 | MS.gene06678:CDS | 45.0% |
| !! | CCATCGACTTCTTCTTCGTT+TGG | - | chr3.3:76633188-76633207 | None:intergenic | 45.0% |
| CTGGATTATTTGCGCATGGG+TGG | + | chr3.3:76633327-76633346 | MS.gene06678:intron | 50.0% | |
| GATTATTTGCGCATGGGTGG+TGG | + | chr3.3:76633330-76633349 | MS.gene06678:intron | 50.0% | |
| GGCATCAATTCGTCCAACTC+CGG | - | chr3.3:76633134-76633153 | None:intergenic | 50.0% | |
| GTTCTGCTACGGCTGTTGGA+CGG | + | chr3.3:76633273-76633292 | MS.gene06678:CDS | 55.0% | |
| ! | GGCTGTTGGACGGAGTGTTC+TGG | + | chr3.3:76633283-76633302 | MS.gene06678:CDS | 60.0% |
| ! | GCGCATGGGTGGTGGCTTTC+TGG | + | chr3.3:76633338-76633357 | MS.gene06678:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 76633024 | 76633637 | 76633024 | ID=MS.gene06678 |
| chr3.3 | mRNA | 76633024 | 76633637 | 76633024 | ID=MS.gene06678.t1;Parent=MS.gene06678 |
| chr3.3 | exon | 76633024 | 76633043 | 76633024 | ID=MS.gene06678.t1.exon1;Parent=MS.gene06678.t1 |
| chr3.3 | CDS | 76633024 | 76633043 | 76633024 | ID=cds.MS.gene06678.t1;Parent=MS.gene06678.t1 |
| chr3.3 | exon | 76633118 | 76633304 | 76633118 | ID=MS.gene06678.t1.exon2;Parent=MS.gene06678.t1 |
| chr3.3 | CDS | 76633118 | 76633304 | 76633118 | ID=cds.MS.gene06678.t1;Parent=MS.gene06678.t1 |
| chr3.3 | exon | 76633395 | 76633637 | 76633395 | ID=MS.gene06678.t1.exon3;Parent=MS.gene06678.t1 |
| chr3.3 | CDS | 76633395 | 76633637 | 76633395 | ID=cds.MS.gene06678.t1;Parent=MS.gene06678.t1 |
| Gene Sequence |
| Protein sequence |
