AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066805

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066805.t1 MTR_4g021760 85.304 313 44 2 1 312 1 312 0.0 548
MS.gene066805.t1 MTR_4g021845 80.184 217 43 0 1 217 1 217 1.61e-128 365
MS.gene066805.t1 MTR_4g021855 73.333 240 64 0 1 240 1 240 9.53e-127 364
MS.gene066805.t1 MTR_4g021790 71.250 240 69 0 1 240 1 240 1.18e-120 347
MS.gene066805.t1 MTR_5g055260 58.515 229 68 4 1 229 1 202 9.44e-86 258
MS.gene066805.t1 MTR_4g023980 76.642 137 32 0 55 191 35 171 2.20e-73 224
MS.gene066805.t1 MTR_6g045327 45.902 305 143 9 1 295 1 293 1.76e-70 223
MS.gene066805.t1 MTR_1g032570 35.556 360 168 6 13 308 14 373 2.61e-66 219
MS.gene066805.t1 MTR_8g079940 39.316 234 116 2 13 220 28 261 2.78e-56 191
MS.gene066805.t1 MTR_3g086100 40.254 236 113 3 13 220 16 251 4.08e-56 191
MS.gene066805.t1 MTR_2g034960 41.379 232 112 2 13 220 32 263 3.72e-55 189
MS.gene066805.t1 MTR_4g121020 40.171 234 114 2 13 220 34 267 1.57e-54 188
MS.gene066805.t1 MTR_4g121020 40.171 234 114 2 13 220 34 267 1.65e-54 188
MS.gene066805.t1 MTR_8g105600 42.291 227 111 2 14 220 13 239 3.81e-52 180
MS.gene066805.t1 MTR_8g093040 44.017 234 110 6 1 229 1 218 8.86e-49 164
MS.gene066805.t1 MTR_1g013170 38.983 236 116 3 13 220 8 243 6.49e-48 167
MS.gene066805.t1 MTR_4g131600 36.626 243 131 4 13 232 23 265 1.04e-47 169
MS.gene066805.t1 MTR_7g117705 34.737 285 153 6 13 267 8 289 2.45e-46 162
MS.gene066805.t1 MTR_3g106220 38.333 240 116 3 13 220 11 250 2.45e-46 166
MS.gene066805.t1 MTR_1g013180 34.495 287 160 4 13 271 159 445 2.39e-45 163
MS.gene066805.t1 MTR_1g032570 32.237 304 143 5 68 308 1 304 2.04e-43 156
MS.gene066805.t1 MTR_3g102590 40.000 210 99 4 13 220 11 195 2.97e-42 146
MS.gene066805.t1 MTR_4g131570 33.198 247 137 4 13 231 20 266 9.85e-42 152
MS.gene066805.t1 MTR_4g131580 33.197 244 138 4 13 231 20 263 2.65e-40 149
MS.gene066805.t1 MTR_2g450070 33.730 252 130 4 13 227 21 272 3.42e-40 149
MS.gene066805.t1 MTR_2g450070 33.730 252 130 4 13 227 21 272 4.62e-40 149
MS.gene066805.t1 MTR_3g102590 39.130 207 99 4 13 217 11 192 9.60e-40 138
MS.gene066805.t1 MTR_3g102600 35.780 218 102 3 4 220 4 184 3.97e-39 137
MS.gene066805.t1 MTR_6g016850 42.767 159 88 2 14 169 13 171 1.75e-38 134
MS.gene066805.t1 MTR_3g102600 34.389 221 107 3 4 223 4 187 1.37e-37 132
MS.gene066805.t1 MTR_3g086100 38.889 180 83 2 68 220 1 180 1.77e-37 139
MS.gene066805.t1 MTR_0450s0040 31.696 224 136 3 14 225 8 226 6.52e-32 122
MS.gene066805.t1 MTR_3g102600 34.731 167 74 1 54 220 4 135 1.35e-31 116
MS.gene066805.t1 MTR_7g026400 32.093 215 113 4 54 247 32 234 3.08e-31 123
MS.gene066805.t1 MTR_3g102600 32.941 170 79 1 54 223 4 138 6.06e-30 111
MS.gene066805.t1 MTR_4g121020 39.873 158 70 2 88 220 6 163 1.72e-29 118
MS.gene066805.t1 MTR_1g013160 33.108 148 72 2 71 191 1 148 1.36e-17 79.0
MS.gene066805.t1 MTR_5g041350 55.556 54 23 1 168 220 15 68 1.45e-13 70.9
MS.gene066805.t1 MTR_7g098250 46.429 84 40 3 168 249 262 342 6.02e-13 69.3
MS.gene066805.t1 MTR_5g014040 36.957 92 54 2 167 256 310 399 7.14e-13 69.3
MS.gene066805.t1 MTR_6g032990 55.556 54 23 1 168 220 42 95 7.45e-13 68.9
MS.gene066805.t1 MTR_4g078810 46.552 58 31 0 167 224 157 214 8.65e-13 68.9
MS.gene066805.t1 MTR_7g088070 46.053 76 31 2 166 231 48 123 1.11e-12 67.4
MS.gene066805.t1 MTR_7g088070 53.571 56 25 1 166 220 48 103 1.39e-12 67.0
MS.gene066805.t1 MTR_2g086450 48.529 68 33 2 154 220 32 98 2.00e-12 67.8
MS.gene066805.t1 MTR_4g081710 51.852 54 25 1 168 220 17 70 2.18e-12 67.4
MS.gene066805.t1 MTR_2g090675 36.264 91 56 1 136 224 127 217 2.97e-12 67.4
MS.gene066805.t1 MTR_7g115530 47.222 72 36 2 168 237 218 289 5.22e-12 65.9
MS.gene066805.t1 MTR_7g115530 47.222 72 36 2 168 237 218 289 7.36e-12 65.5
MS.gene066805.t1 MTR_2g027860 57.407 54 22 1 168 220 49 102 8.80e-12 65.5
MS.gene066805.t1 MTR_4g098870 50.000 54 27 0 167 220 309 362 1.34e-11 65.5
MS.gene066805.t1 MTR_7g115530 50.000 64 30 2 168 229 218 281 1.45e-11 64.7
MS.gene066805.t1 MTR_4g098870 50.000 54 27 0 167 220 309 362 1.53e-11 65.5
MS.gene066805.t1 MTR_4g098870 50.000 54 27 0 167 220 329 382 1.53e-11 65.5
MS.gene066805.t1 MTR_4g098870 50.000 54 27 0 167 220 348 401 1.55e-11 65.5
MS.gene066805.t1 MTR_4g098870 50.000 54 27 0 167 220 329 382 1.56e-11 65.5
MS.gene066805.t1 MTR_7g115530 47.222 72 36 2 168 237 218 289 1.60e-11 65.1
MS.gene066805.t1 MTR_7g089010 46.269 67 31 1 154 220 82 143 1.71e-11 64.3
MS.gene066805.t1 MTR_7g093030 49.123 57 28 1 165 220 209 265 2.56e-11 63.5
MS.gene066805.t1 MTR_1g080330 43.836 73 39 2 159 229 166 238 2.73e-11 63.5
MS.gene066805.t1 MTR_8g024260 28.689 122 83 2 14 131 36 157 2.82e-11 64.7
MS.gene066805.t1 MTR_1g080330 47.619 63 32 1 159 220 166 228 4.60e-11 63.5
MS.gene066805.t1 MTR_1g093080 46.575 73 31 2 149 220 199 264 4.72e-11 63.5
MS.gene066805.t1 MTR_7g093030 49.123 57 28 1 165 220 209 265 5.51e-11 63.2
MS.gene066805.t1 MTR_4g064730 46.552 58 31 0 167 224 130 187 6.39e-11 62.8
MS.gene066805.t1 MTR_5g014040 35.052 97 54 3 167 256 310 404 7.58e-11 63.2
MS.gene066805.t1 MTR_1g053835 46.552 58 30 1 164 220 254 311 8.34e-11 62.8
MS.gene066805.t1 MTR_5g014040 35.052 97 54 3 167 256 270 364 8.55e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066805.t1 AT2G01760 38.039 255 119 5 13 230 10 262 1.79e-53 179
MS.gene066805.t1 AT1G67710 40.928 237 111 4 13 220 10 246 6.75e-53 181
MS.gene066805.t1 AT3G16857 38.189 254 111 5 13 220 36 289 1.52e-48 172
MS.gene066805.t1 AT3G16857 38.189 254 111 5 13 220 36 289 1.87e-48 172
MS.gene066805.t1 AT2G01760 34.276 283 119 6 13 230 10 290 1.55e-47 164
MS.gene066805.t1 AT4G31920 36.032 247 141 5 13 246 16 258 3.59e-45 161
MS.gene066805.t1 AT2G25180 36.207 232 124 3 13 220 16 247 2.25e-42 154
MS.gene066805.t1 AT5G58080 37.931 232 118 5 13 220 17 246 2.77e-38 143
MS.gene066805.t1 AT3G16857 36.697 218 92 5 49 220 2 219 3.21e-37 140
MS.gene066805.t1 AT2G01760 35.678 199 90 4 68 230 1 197 4.60e-36 132
MS.gene066805.t1 AT1G67710 40.331 181 80 3 68 220 1 181 7.44e-34 129
MS.gene066805.t1 AT5G49240 29.289 239 136 3 9 217 37 272 4.76e-29 113
MS.gene066805.t1 AT1G49190 29.237 236 136 5 16 220 36 271 2.06e-26 108
MS.gene066805.t1 AT1G67710 39.130 161 70 3 88 220 12 172 6.57e-26 107
MS.gene066805.t1 AT3G62670 28.689 244 149 6 6 226 33 274 9.43e-26 106
MS.gene066805.t1 AT5G07210 27.969 261 132 8 14 220 16 274 2.96e-23 100
MS.gene066805.t1 AT5G07210 27.138 269 132 9 14 220 16 282 8.64e-21 93.2
MS.gene066805.t1 AT2G27070 25.000 264 139 9 14 220 16 277 3.27e-17 82.4
MS.gene066805.t1 AT5G29000 35.714 140 74 5 103 233 166 298 1.69e-16 79.0
MS.gene066805.t1 AT5G29000 33.962 159 89 5 103 252 123 274 2.13e-16 79.3
MS.gene066805.t1 AT5G29000 33.962 159 89 5 103 252 123 274 2.13e-16 79.3
MS.gene066805.t1 AT5G29000 33.962 159 89 5 103 252 166 317 2.17e-16 79.7
MS.gene066805.t1 AT1G49190 31.933 119 80 1 16 133 36 154 6.31e-15 75.9
MS.gene066805.t1 AT5G44190 50.000 60 30 0 165 224 144 203 1.00e-13 71.6
MS.gene066805.t1 AT5G44190 50.000 60 30 0 165 224 144 203 1.14e-13 71.2
MS.gene066805.t1 AT5G18240 49.296 71 34 2 151 220 29 98 2.27e-13 70.5
MS.gene066805.t1 AT5G18240 49.296 71 34 2 151 220 29 98 2.27e-13 70.5
MS.gene066805.t1 AT5G18240 49.296 71 34 2 151 220 29 98 2.62e-13 70.5
MS.gene066805.t1 AT5G18240 49.296 71 34 2 151 220 29 98 2.64e-13 70.5
MS.gene066805.t1 AT5G18240 49.296 71 34 2 151 220 29 98 2.64e-13 70.5
MS.gene066805.t1 AT3G04030 55.556 54 23 1 168 220 45 98 4.34e-13 69.7
MS.gene066805.t1 AT3G04030 55.556 54 23 1 168 220 45 98 4.82e-13 69.7
MS.gene066805.t1 AT4G28610 47.619 84 40 3 168 249 225 306 1.05e-12 68.6
MS.gene066805.t1 AT3G04450 50.000 58 28 1 164 220 234 291 1.20e-12 67.8
MS.gene066805.t1 AT2G01060 50.769 65 31 1 157 220 4 68 1.51e-12 67.4
MS.gene066805.t1 AT3G04450 50.000 58 28 1 164 220 234 291 2.24e-12 67.8
MS.gene066805.t1 AT5G59570 43.056 72 38 1 149 220 125 193 2.54e-12 66.6
MS.gene066805.t1 AT5G59570 43.056 72 38 1 149 220 125 193 2.54e-12 66.6
MS.gene066805.t1 AT3G04030 52.830 53 25 0 168 220 45 97 2.61e-12 67.4
MS.gene066805.t1 AT3G04450 50.000 58 28 1 164 220 194 251 2.65e-12 67.4
MS.gene066805.t1 AT3G04450 50.000 58 28 1 164 220 194 251 2.65e-12 67.4
MS.gene066805.t1 AT5G60100 30.894 123 79 3 14 131 64 185 4.15e-12 67.0
MS.gene066805.t1 AT2G20570 48.276 58 30 0 167 224 152 209 4.17e-12 66.6
MS.gene066805.t1 AT5G60100 30.894 123 79 3 14 131 64 185 4.78e-12 66.6
MS.gene066805.t1 AT5G60100 30.894 123 79 3 14 131 64 185 4.78e-12 66.6
MS.gene066805.t1 AT5G60100 30.894 123 79 3 14 131 64 185 4.78e-12 66.6
MS.gene066805.t1 AT5G60100 30.894 123 79 3 14 131 64 185 5.13e-12 66.6
MS.gene066805.t1 AT5G60100 30.894 123 79 3 14 131 64 185 5.13e-12 66.6
MS.gene066805.t1 AT5G60100 30.894 123 79 3 14 131 64 185 5.13e-12 66.6
MS.gene066805.t1 AT2G40970 46.269 67 31 1 154 220 96 157 5.60e-12 65.1
MS.gene066805.t1 AT1G69580 51.852 54 25 1 168 220 34 87 9.34e-12 65.5
MS.gene066805.t1 AT5G05090 41.176 85 38 2 136 220 61 133 1.02e-11 64.7
MS.gene066805.t1 AT1G69580 51.852 54 25 1 168 220 34 87 1.06e-11 65.1
MS.gene066805.t1 AT5G24470 27.778 126 81 3 13 131 49 171 1.46e-11 65.5
MS.gene066805.t1 AT3G46640 41.558 77 42 1 144 220 123 196 1.85e-11 64.3
MS.gene066805.t1 AT3G46640 41.558 77 42 1 144 220 123 196 1.85e-11 64.3
MS.gene066805.t1 AT3G10760 46.269 67 31 1 154 220 96 157 1.94e-11 64.3
MS.gene066805.t1 AT3G46640 41.558 77 42 1 144 220 123 196 2.14e-11 64.3
MS.gene066805.t1 AT2G20570 49.091 55 28 0 170 224 171 225 3.23e-11 64.3
MS.gene066805.t1 AT4G18020 38.158 76 44 1 150 225 281 353 4.61e-11 63.9
MS.gene066805.t1 AT4G18020 38.158 76 44 1 150 225 281 353 4.61e-11 63.9
MS.gene066805.t1 AT4G18020 38.158 76 44 1 150 225 281 353 4.61e-11 63.9
MS.gene066805.t1 AT4G18020 38.158 76 44 1 150 225 281 353 4.61e-11 63.9
MS.gene066805.t1 AT4G18020 38.158 76 44 1 150 225 281 353 4.61e-11 63.9
MS.gene066805.t1 AT4G18020 38.158 76 44 1 150 225 281 353 4.61e-11 63.9
MS.gene066805.t1 AT4G18020 38.158 76 44 1 150 225 281 353 4.89e-11 63.9
MS.gene066805.t1 AT4G18020 38.158 76 44 1 150 225 281 353 4.89e-11 63.9
MS.gene066805.t1 AT4G18020 38.158 76 44 1 150 225 281 353 4.89e-11 63.9
MS.gene066805.t1 AT4G18020 38.158 76 44 1 150 225 281 353 4.89e-11 63.9
MS.gene066805.t1 AT4G18020 38.158 76 44 1 150 225 281 353 4.89e-11 63.9
MS.gene066805.t1 AT5G06800 45.312 64 34 1 158 220 182 245 4.92e-11 62.8
MS.gene066805.t1 AT5G42630 32.414 145 81 6 111 251 59 190 7.75e-11 61.6

Find 56 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATGTTGCCAGCCATTTAC+AGG 0.283862 4.2:-80880049 MS.gene066805:intron
GTGTAATCGATGCAATTATA+AGG 0.312923 4.2:-80880871 MS.gene066805:CDS
ACATCTCCTCGACGAAATTA+AGG 0.321564 4.2:+80880892 None:intergenic
CAACAACATTGGGTGGAAAT+TGG 0.345706 4.2:-80879385 MS.gene066805:CDS
TGAGTTGTAGGGTAACATTT+TGG 0.373812 4.2:+80880631 None:intergenic
CGAATATTGGACCAAGCCTT+TGG 0.374007 4.2:-80880442 MS.gene066805:CDS
GATAAATCATCATCAACATA+TGG 0.380393 4.2:+80879313 None:intergenic
AATGTGGGGCTTGCGAATAT+TGG 0.387677 4.2:-80880455 MS.gene066805:CDS
CATCTTACCTCTTGTCTCAT+CGG 0.413533 4.2:+80879534 None:intergenic
ATCAGAAGCCATGCTACATT+TGG 0.418312 4.2:+80880740 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.418636 4.2:-80880272 MS.gene066805:CDS
GTTTCAAGAAAATAGAAAAC+GGG 0.418887 4.2:-80880331 MS.gene066805:CDS
CTTTCTTTCCAACATGTTGA+AGG 0.419409 4.2:+80880716 None:intergenic
TGGCAACATGTTATAAGAAA+AGG 0.422808 4.2:-80880396 MS.gene066805:CDS
TGATGGTTTGTCGGTGAAAA+GGG 0.427097 4.2:+80880962 None:intergenic
AAAAGTGTTGCTCATAATGT+TGG 0.434928 4.2:+80879357 None:intergenic
CAGAATTATGAAGAAAACTT+TGG 0.439068 4.2:+80879437 None:intergenic
ATATGAAATTGTTGGAAGCT+TGG 0.446291 4.2:-80880355 MS.gene066805:CDS
AAAAGAAACACTTGCAAAGA+AGG 0.446990 4.2:-80880286 MS.gene066805:CDS
TCAACATATGGGAACAAGTT+GGG 0.453000 4.2:+80879325 None:intergenic
ATGATGGTTTGTCGGTGAAA+AGG 0.455733 4.2:+80880961 None:intergenic
CTTCAACATGTTGGAAAGAA+AGG 0.459586 4.2:-80880715 MS.gene066805:CDS
AATAAATTCTTACTAATGAC+AGG 0.461511 4.2:+80880603 None:intergenic
TTCTCGCGACCAAGACAAAC+GGG 0.470376 4.2:+80880263 None:intergenic
TTGCAATGCACAACAACATT+GGG 0.475265 4.2:-80879395 MS.gene066805:CDS
TGTAATCGATGCAATTATAA+GGG 0.476930 4.2:-80880870 MS.gene066805:intron
TTGAGACCTTCTGATAATGA+TGG 0.479264 4.2:+80880945 None:intergenic
TACTCACTCAATACCGGAGC+AGG 0.481604 4.2:-80879461 MS.gene066805:CDS
TTCTGATAATGATGGTTTGT+CGG 0.493949 4.2:+80880953 None:intergenic
AAGTCGTAAACATCCATGTT+TGG 0.494866 4.2:+80880657 None:intergenic
TGTTTCAAGAAAATAGAAAA+CGG 0.510078 4.2:-80880332 MS.gene066805:CDS
AGGAAAGAATCATACTATCA+AGG 0.510494 4.2:+80880202 None:intergenic
ATTATGAAGTCTATTGAATG+TGG 0.513075 4.2:-80880471 MS.gene066805:CDS
AAGCTAAATTGTGTTTCAAG+AGG 0.513615 4.2:+80879493 None:intergenic
AGAAAACTTTGGTCCTGCTC+CGG 0.527471 4.2:+80879448 None:intergenic
TTCTGATGCCTTCAACATGT+TGG 0.527887 4.2:-80880724 MS.gene066805:CDS
TTATGAAGTCTATTGAATGT+GGG 0.532174 4.2:-80880470 MS.gene066805:CDS
AAAACGAACCTGTAAATGGC+TGG 0.538065 4.2:+80880041 None:intergenic
TTCAGTTACCAAATGTAGCA+TGG 0.538628 4.2:-80880748 MS.gene066805:intron
ATTGCAATGCACAACAACAT+TGG 0.541421 4.2:-80879396 MS.gene066805:CDS
ATTCTCGCGACCAAGACAAA+CGG 0.543227 4.2:+80880262 None:intergenic
CTACGTGCAGTCAATCAACT+AGG 0.547867 4.2:-80880225 MS.gene066805:CDS
ATAAATCATCATCAACATAT+GGG 0.548211 4.2:+80879314 None:intergenic
ATCAACATATGGGAACAAGT+TGG 0.552596 4.2:+80879324 None:intergenic
AGAAGCTACTCACTCAATAC+CGG 0.567403 4.2:-80879467 MS.gene066805:CDS
ATCATTATCAGAAGGTCTCA+AGG 0.582734 4.2:-80880943 MS.gene066805:CDS
CAATGCACAACAACATTGGG+TGG 0.593262 4.2:-80879392 MS.gene066805:CDS
AGAACTTTCTGCAGAATCAA+CGG 0.593830 4.2:+80880494 None:intergenic
TCTTATAACATGTTGCCACA+TGG 0.594344 4.2:+80880401 None:intergenic
TATGAAGTCTATTGAATGTG+GGG 0.598969 4.2:-80880469 MS.gene066805:CDS
TGATGCTCATATGCCAAACA+TGG 0.604750 4.2:-80880670 MS.gene066805:CDS
CACTCTCCTTAATTTCGTCG+AGG 0.625060 4.2:-80880898 MS.gene066805:CDS
GACAAACCATCATTATCAGA+AGG 0.636966 4.2:-80880951 MS.gene066805:CDS
AGCTCAACCGATGAGACAAG+AGG 0.641822 4.2:-80879541 MS.gene066805:CDS
ATGATTTATCATCACTTGTG+TGG 0.696325 4.2:-80879300 MS.gene066805:CDS
TTTCAAGAAAATAGAAAACG+GGG 0.722642 4.2:-80880330 MS.gene066805:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAAAATATGAAATTGT+TGG - chr4.2:80879900-80879919 MS.gene066805:intron 15.0%
!! AACATAGTAAAAAAGTAATT+AGG + chr4.2:80880084-80880103 None:intergenic 15.0%
!! AATAAATTCTTACTAATGAC+AGG + chr4.2:80879663-80879682 None:intergenic 20.0%
!! ATAAATCATCATCAACATAT+GGG + chr4.2:80880952-80880971 None:intergenic 20.0%
!! ATAGATGAAAACTTTGAATA+AGG + chr4.2:80879436-80879455 None:intergenic 20.0%
!! TGAAAAATAAGAGAAAAACA+TGG + chr4.2:80880138-80880157 None:intergenic 20.0%
!! TGATTGTAAACTGAAATTTA+TGG - chr4.2:80880633-80880652 MS.gene066805:CDS 20.0%
!! TGTTTCAAGAAAATAGAAAA+CGG - chr4.2:80879931-80879950 MS.gene066805:intron 20.0%
!!! AAAAAAGATTCTGAAAATCA+TGG - chr4.2:80880169-80880188 MS.gene066805:intron 20.0%
!!! AACATGTTCTTTTTTCATAT+CGG + chr4.2:80880201-80880220 None:intergenic 20.0%
!!! ATGATTTTCAGAATCTTTTT+TGG + chr4.2:80880170-80880189 None:intergenic 20.0%
!!! ATGTTTTTCTCTTATTTTTC+AGG - chr4.2:80880137-80880156 MS.gene066805:intron 20.0%
!!! TGTTTTTCTCTTATTTTTCA+GGG - chr4.2:80880138-80880157 MS.gene066805:intron 20.0%
!!! TTATTTCTTTTTTGTAGTGA+TGG - chr4.2:80879746-80879765 MS.gene066805:intron 20.0%
!!! TTCAAAGTTTTCATCTATTT+TGG - chr4.2:80879438-80879457 MS.gene066805:CDS 20.0%
! AGTAGTATATTATCAACAAG+AGG + chr4.2:80880597-80880616 None:intergenic 25.0%
! ATGCATAAGCTATTAAATTC+AGG + chr4.2:80880454-80880473 None:intergenic 25.0%
! ATTATGAAGTCTATTGAATG+TGG - chr4.2:80879792-80879811 MS.gene066805:intron 25.0%
! CAGAATTATGAAGAAAACTT+TGG + chr4.2:80880829-80880848 None:intergenic 25.0%
! GATAAATCATCATCAACATA+TGG + chr4.2:80880953-80880972 None:intergenic 25.0%
! GTTTCAAGAAAATAGAAAAC+GGG - chr4.2:80879932-80879951 MS.gene066805:intron 25.0%
! TAACAGCTAGAAAAAGAATT+TGG + chr4.2:80880488-80880507 None:intergenic 25.0%
! TGTAATCGATGCAATTATAA+GGG - chr4.2:80879393-80879412 MS.gene066805:CDS 25.0%
! TTATGAAGTCTATTGAATGT+GGG - chr4.2:80879793-80879812 MS.gene066805:intron 25.0%
! TTTCAAGAAAATAGAAAACG+GGG - chr4.2:80879933-80879952 MS.gene066805:intron 25.0%
!!! ACATGTTCTTTTTTCATATC+GGG + chr4.2:80880200-80880219 None:intergenic 25.0%
!!! GTTTTTCTCTTATTTTTCAG+GGG - chr4.2:80880139-80880158 MS.gene066805:intron 25.0%
AAAAGAAACACTTGCAAAGA+AGG - chr4.2:80879977-80879996 MS.gene066805:intron 30.0%
AAGCTAAATTGTGTTTCAAG+AGG + chr4.2:80880773-80880792 None:intergenic 30.0%
AGAGGATATATAGAAGCTAA+CGG + chr4.2:80880579-80880598 None:intergenic 30.0%
AGGAAAGAATCATACTATCA+AGG + chr4.2:80880064-80880083 None:intergenic 30.0%
ATATGAAATTGTTGGAAGCT+TGG - chr4.2:80879908-80879927 MS.gene066805:intron 30.0%
ATGATTTATCATCACTTGTG+TGG - chr4.2:80880963-80880982 MS.gene066805:CDS 30.0%
GTGTAATCGATGCAATTATA+AGG - chr4.2:80879392-80879411 MS.gene066805:CDS 30.0%
TACACAATTACAACAGACTT+AGG + chr4.2:80880326-80880345 None:intergenic 30.0%
TATGAAGTCTATTGAATGTG+GGG - chr4.2:80879794-80879813 MS.gene066805:intron 30.0%
TGGCAACATGTTATAAGAAA+AGG - chr4.2:80879867-80879886 MS.gene066805:intron 30.0%
TTGTATAACATGCAAGACTA+TGG + chr4.2:80880369-80880388 None:intergenic 30.0%
! CTTTGCAAGTGTTTCTTTTA+AGG + chr4.2:80879976-80879995 None:intergenic 30.0%
! TTCTGATAATGATGGTTTGT+CGG + chr4.2:80879313-80879332 None:intergenic 30.0%
!! AAAAGTGTTGCTCATAATGT+TGG + chr4.2:80880909-80880928 None:intergenic 30.0%
!! TTGAACTGTTTTTCAACCAA+AGG + chr4.2:80879840-80879859 None:intergenic 30.0%
AAAAACAGTTCAAGACCATG+TGG - chr4.2:80879847-80879866 MS.gene066805:intron 35.0%
AAAAGATCACTGACAAACCT+AGG + chr4.2:80880302-80880321 None:intergenic 35.0%
AAGTCGTAAACATCCATGTT+TGG + chr4.2:80879609-80879628 None:intergenic 35.0%
AGAACTTTCTGCAGAATCAA+CGG + chr4.2:80879772-80879791 None:intergenic 35.0%
ATCAACATATGGGAACAAGT+TGG + chr4.2:80880942-80880961 None:intergenic 35.0%
ATCATTATCAGAAGGTCTCA+AGG - chr4.2:80879320-80879339 MS.gene066805:CDS 35.0%
ATTGCAATGCACAACAACAT+TGG - chr4.2:80880867-80880886 MS.gene066805:intron 35.0%
CTTCAACATGTTGGAAAGAA+AGG - chr4.2:80879548-80879567 MS.gene066805:CDS 35.0%
CTTTCTTTCCAACATGTTGA+AGG + chr4.2:80879550-80879569 None:intergenic 35.0%
GACAAACCATCATTATCAGA+AGG - chr4.2:80879312-80879331 MS.gene066805:CDS 35.0%
TCAACATATGGGAACAAGTT+GGG + chr4.2:80880941-80880960 None:intergenic 35.0%
TCTTATAACATGTTGCCACA+TGG + chr4.2:80879865-80879884 None:intergenic 35.0%
TGCAAAAACGAACCTGTAAA+TGG + chr4.2:80880229-80880248 None:intergenic 35.0%
TTCAGTTACCAAATGTAGCA+TGG - chr4.2:80879515-80879534 MS.gene066805:CDS 35.0%
TTGCAATGCACAACAACATT+GGG - chr4.2:80880868-80880887 MS.gene066805:intron 35.0%
! TGAGTTGTAGGGTAACATTT+TGG + chr4.2:80879635-80879654 None:intergenic 35.0%
! TTGAGACCTTCTGATAATGA+TGG + chr4.2:80879321-80879340 None:intergenic 35.0%
!!! GACAGGAATTTTGAGTTGTA+GGG + chr4.2:80879646-80879665 None:intergenic 35.0%
!!! TGACAGGAATTTTGAGTTGT+AGG + chr4.2:80879647-80879666 None:intergenic 35.0%
AAAACGAACCTGTAAATGGC+TGG + chr4.2:80880225-80880244 None:intergenic 40.0%
AAACCTAGGCAACTAGACTT+AGG + chr4.2:80880288-80880307 None:intergenic 40.0%
ACATCTCCTCGACGAAATTA+AGG + chr4.2:80879374-80879393 None:intergenic 40.0%
AGAAGCTACTCACTCAATAC+CGG - chr4.2:80880796-80880815 MS.gene066805:intron 40.0%
ATCAGAAGCCATGCTACATT+TGG + chr4.2:80879526-80879545 None:intergenic 40.0%
CAACAACATTGGGTGGAAAT+TGG - chr4.2:80880878-80880897 MS.gene066805:CDS 40.0%
CAGGTTCGTTTCATTTACAC+CGG + chr4.2:80880435-80880454 None:intergenic 40.0%
CATCTTACCTCTTGTCTCAT+CGG + chr4.2:80880732-80880751 None:intergenic 40.0%
GAGTAGCTTCAGCTACTTAT+AGG - chr4.2:80880409-80880428 MS.gene066805:CDS 40.0%
TGATGCTCATATGCCAAACA+TGG - chr4.2:80879593-80879612 MS.gene066805:intron 40.0%
TTCTGATGCCTTCAACATGT+TGG - chr4.2:80879539-80879558 MS.gene066805:CDS 40.0%
! ATGATGGTTTGTCGGTGAAA+AGG + chr4.2:80879305-80879324 None:intergenic 40.0%
! TGATGGTTTGTCGGTGAAAA+GGG + chr4.2:80879304-80879323 None:intergenic 40.0%
!! CTGTTTTTCAACCAAAGGCT+TGG + chr4.2:80879835-80879854 None:intergenic 40.0%
AATGTGGGGCTTGCGAATAT+TGG - chr4.2:80879808-80879827 MS.gene066805:intron 45.0%
ACATGTTGCCAGCCATTTAC+AGG - chr4.2:80880214-80880233 MS.gene066805:intron 45.0%
AGAAAACTTTGGTCCTGCTC+CGG + chr4.2:80880818-80880837 None:intergenic 45.0%
AGCTTCAGCTACTTATAGGC+CGG - chr4.2:80880413-80880432 MS.gene066805:CDS 45.0%
ATTCTCGCGACCAAGACAAA+CGG + chr4.2:80880004-80880023 None:intergenic 45.0%
CAATGCACAACAACATTGGG+TGG - chr4.2:80880871-80880890 MS.gene066805:CDS 45.0%
CACTCTCCTTAATTTCGTCG+AGG - chr4.2:80879365-80879384 MS.gene066805:CDS 45.0%
CGAATATTGGACCAAGCCTT+TGG - chr4.2:80879821-80879840 MS.gene066805:intron 45.0%
CTACGTGCAGTCAATCAACT+AGG - chr4.2:80880038-80880057 MS.gene066805:intron 45.0%
GTACCTAAGTCTAGTTGCCT+AGG - chr4.2:80880282-80880301 MS.gene066805:CDS 45.0%
AGCTCAACCGATGAGACAAG+AGG - chr4.2:80880722-80880741 MS.gene066805:CDS 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG - chr4.2:80879991-80880010 MS.gene066805:intron 50.0%
TACTCACTCAATACCGGAGC+AGG - chr4.2:80880802-80880821 MS.gene066805:intron 50.0%
TTCTCGCGACCAAGACAAAC+GGG + chr4.2:80880003-80880022 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 80879297 80880988 80879297 ID=MS.gene066805
chr4.2 mRNA 80879297 80880988 80879297 ID=MS.gene066805.t1;Parent=MS.gene066805
chr4.2 exon 80880871 80880988 80880871 ID=MS.gene066805.t1.exon1;Parent=MS.gene066805.t1
chr4.2 CDS 80880871 80880988 80880871 ID=cds.MS.gene066805.t1;Parent=MS.gene066805.t1
chr4.2 exon 80880616 80880765 80880616 ID=MS.gene066805.t1.exon2;Parent=MS.gene066805.t1
chr4.2 CDS 80880616 80880765 80880616 ID=cds.MS.gene066805.t1;Parent=MS.gene066805.t1
chr4.2 exon 80880217 80880522 80880217 ID=MS.gene066805.t1.exon3;Parent=MS.gene066805.t1
chr4.2 CDS 80880217 80880522 80880217 ID=cds.MS.gene066805.t1;Parent=MS.gene066805.t1
chr4.2 exon 80880050 80880126 80880050 ID=MS.gene066805.t1.exon4;Parent=MS.gene066805.t1
chr4.2 CDS 80880050 80880126 80880050 ID=cds.MS.gene066805.t1;Parent=MS.gene066805.t1
chr4.2 exon 80879297 80879584 80879297 ID=MS.gene066805.t1.exon5;Parent=MS.gene066805.t1
chr4.2 CDS 80879297 80879584 80879297 ID=cds.MS.gene066805.t1;Parent=MS.gene066805.t1
Gene Sequence

>MS.gene066805

ATGGCCCTTTTCACCGACAAACCATCATTATCAGAAGGTCTCAAGGTTCTTGCAATTGATCATGACATCACTCTCCTTAATTTCGTCGAGGAGATGTGTAATCGATGCAATTATAAGGGTTAGTAACTGAATCACACCTTATTCAAAGTTTTCATCTATTTTGGTAAAAATTCTGACTTTTATATGTTTTCATGTTTGCTTATTCTGATATTGTTTATTTCAGTTACCAAATGTAGCATGGCTTCTGATGCCTTCAACATGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTGATGCTCATATGCCAAACATGGATGTTTACGACTTTGTCCAAAATGTTACCCTACAACTCAAAATTCCTGTCATTAGTAAGAATTTATTCAACTTTGCACAATTCAAATGATTCATTCATATTATGTACTTAATAAGTGTGAACTTACATTATTATTTCTTTTTTGTAGTGATGGCCGTTGATTCTGCAGAAAGTTCTATTATGAAGTCTATTGAATGTGGGGCTTGCGAATATTGGACCAAGCCTTTGGTTGAAAAACAGTTCAAGACCATGTGGCAACATGTTATAAGAAAAGGTTTGACTCAAAACAAAAAATATGAAATTGTTGGAAGCTTGGTGTTTCAAGAAAATAGAAAACGGGGTAGAGAAGATGCTAATGCCTTAAAAGAAACACTTGCAAAGAAGGCCCGTTTGTCTTGGTCGCGAGAATTGCATCAGCAGTTTCTACGTGCAGTCAATCAACTAGGCCTTGATAGTATGATTCTTTCCTAATTACTTTTTTACTATGTTTTATTGCATTCTTCAACAGTTGAACTTATGTCCATGTTTTTCTCTTATTTTTCAGGGGCAACGCCAAAAAAGATTCTGAAAATCATGGATGTTCCCGATATGAAAAAAGAACATGTTGCCAGCCATTTACAGGTTCGTTTTTGCATAAATTTTATTGATTCTGTAAATTTGATATATTGTACCTAAGTCTAGTTGCCTAGGTTTGTCAGTGATCTTTTACCTAAGTCTGTTGTAATTGTGTAAATCATGTATGTTGTTTATACCATAGTCTTGCATGTTATACAACTTATAACAGTTATAATTGAGAGTAGCTTCAGCTACTTATAGGCCGGTGTAAATGAAACGAACCTGAATTTAATAGCTTATGCATGACATGTAATTCCAAATTCTTTTTCTAGCTGTTACAGCTTGCATTAATCATTTGCAGTGACTATTTCTTGTTGTATTGCTAGAATAGAATATAAGTATTTGTCCGTTAGCTTCTATATATCCTCTTGTTGATAATATACTACTTGTGTTCAATTTTGTATGATTGTAAACTGAAATTTATGGAGCTAGTATTATTATTAACATTGTAACTGCAATGTTGTTTTGCAGAAGTATAGAAATCAGTTTAAGAGCTCAACCGATGAGACAAGAGGTAAGATGAAAAAATCAAAGAAGAGTCCTCTTGAAACACAATTTAGCTTAGAAGAAGCTACTCACTCAATACCGGAGCAGGACCAAAGTTTTCTTCATAATTCTGCGACACAATGTCGCGACAATTGCAATGCACAACAACATTGGGTGGAAATTGGTGAATCCAACATTATGAGCAACACTTTTTCTGACTTTCCCAACTTGTTCCCATATGTTGATGATGATTTATCATCACTTGTGTGGTGA
Protein sequence

>MS.gene066805.t1

MALFTDKPSLSEGLKVLAIDHDITLLNFVEEMCNRCNYKVTKCSMASDAFNMLERKDCFDVMLIDAHMPNMDVYDFVQNVTLQLKIPVIMMAVDSAESSIMKSIECGACEYWTKPLVEKQFKTMWQHVIRKGLTQNKKYEIVGSLVFQENRKRGREDANALKETLAKKARLSWSRELHQQFLRAVNQLGLDRATPKKILKIMDVPDMKKEHVASHLQKYRNQFKSSTDETRGKMKKSKKSPLETQFSLEEATHSIPEQDQSFLHNSATQCRDNCNAQQHWVEIGESNIMSNTFSDFPNLFPYVDDDLSSLVW