AlfalfaGEDB | MS.gene066807

M. sativa cultivar XinJiangDaYe / MS.gene066807

KEGG: -
GO: GO:0000160 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0007165 GO:0008150 GO:0009987 GO:0035556 GO:0050789 GO:0050794 GO:0065007 GO:0097159 GO:1901363
PFAM: PF00072.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066807.t1	XP_013455011.1	88.7	274	31	0	1	274	1	274	1.30E-136	495.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066807.t1	Q5N6V8	39.8	249	122	4	5	225	1	249	2.0e-43	177.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066807.t1	A0A072UH21	88.7	274	31	0	1	274	1	274	9.7e-137	495.7

TFs/TRs:

Gene ID	Type	Classification
MS.gene066807.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066807.t1	MTR_4g021790	88.686	274	31	0	1	274	1	274	0.0	507
MS.gene066807.t1	MTR_4g021855	77.741	301	40	1	1	274	1	301	6.02e-172	477
MS.gene066807.t1	MTR_4g021845	85.253	217	32	0	1	217	1	217	1.70e-136	384
MS.gene066807.t1	MTR_4g021760	78.423	241	51	1	1	240	1	241	5.39e-136	386
MS.gene066807.t1	MTR_5g055260	65.267	262	64	4	1	262	1	235	1.73e-108	314
MS.gene066807.t1	MTR_4g023980	77.372	137	31	0	55	191	35	171	2.99e-75	227
MS.gene066807.t1	MTR_1g032570	47.368	247	99	3	10	225	11	257	1.88e-68	223
MS.gene066807.t1	MTR_6g045327	45.992	237	117	4	10	244	14	241	3.55e-57	188
MS.gene066807.t1	MTR_8g105600	42.917	240	117	2	10	229	9	248	1.02e-56	191
MS.gene066807.t1	MTR_4g121020	41.036	251	118	3	10	232	31	279	2.82e-55	189
MS.gene066807.t1	MTR_4g121020	41.036	251	118	3	10	232	31	279	3.03e-55	189
MS.gene066807.t1	MTR_2g034960	41.667	240	116	2	10	225	29	268	6.33e-55	187
MS.gene066807.t1	MTR_3g086100	39.695	262	128	4	10	241	13	274	7.88e-55	186
MS.gene066807.t1	MTR_8g079940	39.918	243	120	2	9	225	24	266	7.52e-54	182
MS.gene066807.t1	MTR_4g131600	38.655	238	123	4	10	224	20	257	2.52e-48	169
MS.gene066807.t1	MTR_1g013170	39.271	247	122	3	7	225	2	248	8.77e-48	166
MS.gene066807.t1	MTR_7g117705	36.179	246	129	4	7	224	2	247	1.06e-44	157
MS.gene066807.t1	MTR_3g106220	37.849	251	118	4	10	225	8	255	1.51e-44	159
MS.gene066807.t1	MTR_6g016850	44.737	152	82	1	10	159	9	160	1.08e-43	146
MS.gene066807.t1	MTR_1g032570	44.149	188	75	2	68	225	1	188	1.38e-43	155
MS.gene066807.t1	MTR_1g013180	36.800	250	130	3	7	228	153	402	1.59e-43	157
MS.gene066807.t1	MTR_8g093040	41.453	234	116	6	1	229	1	218	3.41e-43	148
MS.gene066807.t1	MTR_4g131570	34.400	250	136	4	10	231	17	266	4.73e-42	152
MS.gene066807.t1	MTR_4g131580	35.223	247	135	3	10	231	17	263	1.04e-41	151
MS.gene066807.t1	MTR_2g450070	33.333	255	133	4	10	227	18	272	1.25e-40	149
MS.gene066807.t1	MTR_2g450070	33.333	255	133	4	10	227	18	272	1.65e-40	149
MS.gene066807.t1	MTR_3g102590	36.916	214	108	4	10	221	8	196	9.01e-39	136
MS.gene066807.t1	MTR_3g102600	35.897	234	113	4	10	242	8	205	3.92e-37	131
MS.gene066807.t1	MTR_3g086100	37.931	203	97	3	68	241	1	203	3.46e-36	135
MS.gene066807.t1	MTR_3g102590	36.190	210	107	4	10	217	8	192	4.80e-36	127
MS.gene066807.t1	MTR_7g026400	36.364	209	100	4	45	229	19	218	4.51e-34	130
MS.gene066807.t1	MTR_3g102600	34.597	211	102	3	10	219	8	183	8.30e-33	119
MS.gene066807.t1	MTR_0450s0040	30.901	233	146	3	12	233	6	234	1.88e-31	119
MS.gene066807.t1	MTR_3g102600	35.602	191	87	3	52	242	2	156	7.48e-30	111
MS.gene066807.t1	MTR_4g121020	38.953	172	76	2	88	232	6	175	4.67e-29	115
MS.gene066807.t1	MTR_3g102600	33.929	168	76	2	52	219	2	134	2.49e-25	98.2
MS.gene066807.t1	MTR_5g041350	51.429	70	33	1	168	236	15	84	3.91e-17	80.5
MS.gene066807.t1	MTR_1g013160	32.432	148	73	2	71	191	1	148	1.20e-16	75.5
MS.gene066807.t1	MTR_6g032990	63.636	55	19	1	168	221	42	96	1.50e-16	79.0
MS.gene066807.t1	MTR_2g086450	60.000	55	21	1	168	221	45	99	2.01e-15	75.9
MS.gene066807.t1	MTR_2g016220	52.703	74	29	3	153	220	69	142	4.14e-15	74.7
MS.gene066807.t1	MTR_2g016220	52.703	74	29	3	153	220	69	142	4.75e-15	74.7
MS.gene066807.t1	MTR_4g064730	50.704	71	34	1	156	225	118	188	6.92e-15	73.6
MS.gene066807.t1	MTR_7g098250	51.429	70	33	1	168	236	262	331	1.78e-14	73.2
MS.gene066807.t1	MTR_7g089010	55.932	59	26	0	167	225	90	148	2.91e-14	71.6
MS.gene066807.t1	MTR_7g115530	52.857	70	30	2	168	234	218	287	2.99e-14	71.6
MS.gene066807.t1	MTR_2g090675	39.785	93	55	1	133	224	125	217	3.95e-14	72.0
MS.gene066807.t1	MTR_7g088070	43.182	88	47	2	159	243	41	128	4.26e-14	70.9
MS.gene066807.t1	MTR_7g115530	52.857	70	30	2	168	234	218	287	4.93e-14	71.2
MS.gene066807.t1	MTR_7g115530	52.857	70	30	2	168	234	218	287	5.97e-14	71.6
MS.gene066807.t1	MTR_4g081710	56.364	55	23	1	168	221	17	71	6.16e-14	71.2
MS.gene066807.t1	MTR_2g016220	46.429	84	38	4	153	229	69	152	6.36e-14	71.2
MS.gene066807.t1	MTR_7g088070	40.206	97	52	2	159	249	41	137	6.47e-14	70.5
MS.gene066807.t1	MTR_1g080330	41.935	93	51	2	158	249	165	255	7.88e-14	70.5
MS.gene066807.t1	MTR_1g080330	44.318	88	46	2	163	249	170	255	8.59e-14	70.9
MS.gene066807.t1	MTR_1g093080	47.500	80	34	2	149	227	199	271	1.38e-13	70.5
MS.gene066807.t1	MTR_7g115530	53.731	67	28	2	168	233	218	282	1.59e-13	70.1
MS.gene066807.t1	MTR_4g078810	55.172	58	26	0	167	224	157	214	2.00e-13	70.1
MS.gene066807.t1	MTR_4g098870	55.357	56	25	0	167	222	329	384	5.17e-13	68.9
MS.gene066807.t1	MTR_4g098870	55.357	56	25	0	167	222	309	364	5.18e-13	68.9
MS.gene066807.t1	MTR_4g086835	51.667	60	29	0	166	225	272	331	5.90e-13	68.6
MS.gene066807.t1	MTR_4g098870	55.357	56	25	0	167	222	329	384	6.80e-13	68.6
MS.gene066807.t1	MTR_4g098870	55.357	56	25	0	167	222	309	364	7.14e-13	68.6
MS.gene066807.t1	MTR_4g098870	55.357	56	25	0	167	222	348	403	7.64e-13	68.6
MS.gene066807.t1	MTR_4g113140	47.368	76	38	2	159	233	227	301	1.16e-12	67.8
MS.gene066807.t1	MTR_0223s0040	53.571	56	25	1	166	220	16	71	1.17e-12	67.0
MS.gene066807.t1	MTR_7g069660	51.786	56	26	1	166	220	69	124	1.34e-12	67.4
MS.gene066807.t1	MTR_1g053835	51.562	64	28	2	159	221	251	312	1.36e-12	67.8
MS.gene066807.t1	MTR_5g014040	46.377	69	37	0	157	225	300	368	1.47e-12	67.8
MS.gene066807.t1	MTR_7g093030	53.571	56	25	1	166	220	210	265	2.26e-12	66.2
MS.gene066807.t1	MTR_1g053800	53.968	63	28	1	162	223	171	233	2.43e-12	66.2
MS.gene066807.t1	MTR_3g450310	53.571	56	25	1	166	220	17	72	2.58e-12	65.1
MS.gene066807.t1	MTR_2g027860	53.425	73	30	3	168	236	49	121	2.67e-12	66.2
MS.gene066807.t1	MTR_7g093030	53.571	56	25	1	166	220	210	265	2.73e-12	66.6
MS.gene066807.t1	MTR_6g444980	49.254	67	33	1	156	221	23	89	3.78e-12	66.2
MS.gene066807.t1	MTR_6g444980	49.254	67	33	1	156	221	23	89	4.33e-12	65.9
MS.gene066807.t1	MTR_7g115530	59.615	52	20	1	168	218	218	269	7.85e-12	64.3
MS.gene066807.t1	MTR_2g023580	53.571	56	25	1	166	220	53	108	8.73e-12	63.9
MS.gene066807.t1	MTR_5g017980	51.667	60	29	0	166	225	191	250	8.97e-12	64.7
MS.gene066807.t1	MTR_5g017980	51.667	60	29	0	166	225	190	249	9.26e-12	64.7
MS.gene066807.t1	MTR_1g112370	45.714	70	35	2	154	220	6	75	9.47e-12	64.7
MS.gene066807.t1	MTR_8g086410	43.284	67	37	1	155	220	96	162	1.16e-11	64.7
MS.gene066807.t1	MTR_8g086410	43.284	67	37	1	155	220	98	164	1.18e-11	64.7
MS.gene066807.t1	MTR_1g090670	53.333	60	27	1	163	221	39	98	1.31e-11	63.9
MS.gene066807.t1	MTR_2g027800	36.207	116	66	3	143	250	11	126	1.67e-11	63.9
MS.gene066807.t1	MTR_5g054300	52.459	61	28	1	167	226	197	257	1.70e-11	63.9
MS.gene066807.t1	MTR_4g086835	50.820	61	29	1	166	225	272	332	2.08e-11	63.9
MS.gene066807.t1	MTR_5g027440	45.714	70	35	2	163	229	20	89	2.24e-11	63.2
MS.gene066807.t1	MTR_2g084230	41.892	74	39	2	148	220	80	150	3.92e-11	62.8
MS.gene066807.t1	MTR_2g084230	41.892	74	39	2	148	220	80	150	4.37e-11	62.8
MS.gene066807.t1	MTR_2g027800	47.761	67	34	1	163	228	34	100	4.67e-11	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066807.t1	AT2G01760	38.129	278	131	5	7	245	4	279	1.41e-56	186
MS.gene066807.t1	AT1G67710	40.530	264	126	5	11	243	8	271	2.01e-51	176
MS.gene066807.t1	AT3G16857	38.007	271	118	4	10	232	33	301	4.76e-51	177
MS.gene066807.t1	AT3G16857	38.007	271	118	4	10	232	33	301	6.39e-51	177
MS.gene066807.t1	AT2G01760	34.641	306	131	6	7	245	4	307	9.56e-51	172
MS.gene066807.t1	AT2G25180	34.629	283	157	4	10	268	13	291	8.63e-44	157
MS.gene066807.t1	AT4G31920	38.627	233	129	4	10	229	13	244	1.27e-43	155
MS.gene066807.t1	AT5G58080	38.272	243	118	6	10	225	14	251	5.21e-39	144
MS.gene066807.t1	AT3G16857	36.638	232	97	4	49	232	2	231	9.32e-39	143
MS.gene066807.t1	AT2G01760	35.648	216	99	4	68	245	1	214	1.53e-35	129
MS.gene066807.t1	AT1G67710	40.860	186	82	3	68	225	1	186	3.50e-33	126
MS.gene066807.t1	AT3G62670	31.982	222	128	5	28	226	53	274	2.01e-32	123
MS.gene066807.t1	AT1G49190	30.645	248	141	5	10	226	30	277	5.32e-32	122
MS.gene066807.t1	AT5G49240	31.780	236	134	5	9	217	37	272	3.37e-31	117
MS.gene066807.t1	AT5G07210	29.259	270	135	7	14	229	16	283	1.94e-26	108
MS.gene066807.t1	AT1G67710	40.361	166	71	3	88	225	12	177	6.93e-26	105
MS.gene066807.t1	AT5G07210	28.417	278	135	8	14	229	16	291	6.75e-24	101
MS.gene066807.t1	AT2G27070	26.022	269	140	7	14	225	16	282	4.05e-19	87.0
MS.gene066807.t1	AT1G49190	33.600	125	82	1	10	133	30	154	9.09e-19	86.3
MS.gene066807.t1	AT1G68210	24.910	277	145	8	8	225	7	279	9.85e-17	80.5
MS.gene066807.t1	AT5G29000	44.681	94	44	3	128	220	198	284	1.80e-16	78.2
MS.gene066807.t1	AT5G29000	37.008	127	65	4	103	221	123	242	2.47e-16	78.2
MS.gene066807.t1	AT5G29000	37.008	127	65	4	103	221	123	242	2.47e-16	78.2
MS.gene066807.t1	AT5G29000	44.211	95	45	3	128	221	198	285	2.63e-16	78.6
MS.gene066807.t1	AT3G04030	61.818	55	20	1	168	221	45	99	3.53e-16	78.2
MS.gene066807.t1	AT3G04030	61.818	55	20	1	168	221	45	99	3.81e-16	77.8
MS.gene066807.t1	AT2G01060	49.398	83	40	2	157	238	4	85	6.90e-16	76.3
MS.gene066807.t1	AT5G18240	61.818	55	20	1	168	221	45	99	7.78e-16	77.0
MS.gene066807.t1	AT5G18240	61.818	55	20	1	168	221	45	99	7.78e-16	77.0
MS.gene066807.t1	AT5G18240	61.818	55	20	1	168	221	45	99	7.83e-16	77.0
MS.gene066807.t1	AT5G18240	61.818	55	20	1	168	221	45	99	7.90e-16	77.0
MS.gene066807.t1	AT5G18240	61.818	55	20	1	168	221	45	99	7.90e-16	77.0
MS.gene066807.t1	AT3G04030	57.407	54	23	0	168	221	45	98	4.09e-15	75.1
MS.gene066807.t1	AT5G59570	50.649	77	35	2	149	225	125	198	9.06e-15	73.2
MS.gene066807.t1	AT5G59570	50.649	77	35	2	149	225	125	198	9.06e-15	73.2
MS.gene066807.t1	AT5G05090	55.932	59	26	0	167	225	80	138	9.32e-15	72.8
MS.gene066807.t1	AT3G46640	43.333	90	48	1	144	233	123	209	1.09e-14	73.2
MS.gene066807.t1	AT3G46640	41.489	94	52	1	144	237	123	213	1.48e-14	72.8
MS.gene066807.t1	AT3G46640	41.489	94	52	1	144	237	123	213	1.48e-14	72.8
MS.gene066807.t1	AT3G10760	55.932	59	26	0	167	225	104	162	2.07e-14	72.4
MS.gene066807.t1	AT2G40970	54.237	59	27	0	167	225	104	162	2.96e-14	71.2
MS.gene066807.t1	AT5G44190	58.621	58	24	0	167	224	146	203	5.42e-14	71.6
MS.gene066807.t1	AT5G44190	58.621	58	24	0	167	224	146	203	6.56e-14	71.2
MS.gene066807.t1	AT3G04450	57.407	54	22	1	168	220	238	291	9.46e-14	71.2
MS.gene066807.t1	AT3G04450	57.407	54	22	1	168	220	198	251	1.02e-13	70.9
MS.gene066807.t1	AT3G04450	57.407	54	22	1	168	220	198	251	1.02e-13	70.9
MS.gene066807.t1	AT3G04450	54.839	62	25	2	160	220	232	291	1.20e-13	70.1
MS.gene066807.t1	AT4G28610	60.000	55	21	1	168	221	225	279	1.67e-13	70.5
MS.gene066807.t1	AT3G24120	37.864	103	55	2	163	256	36	138	1.80e-13	69.3
MS.gene066807.t1	AT3G24120	37.864	103	55	2	163	256	36	138	1.92e-13	69.3
MS.gene066807.t1	AT4G13640	39.623	106	45	3	163	267	32	119	2.81e-13	68.9
MS.gene066807.t1	AT4G13640	39.623	106	45	3	163	267	32	119	2.97e-13	68.9
MS.gene066807.t1	AT1G79430	40.566	106	57	2	149	253	15	115	3.41e-13	69.3
MS.gene066807.t1	AT1G69580	49.231	65	32	1	168	231	34	98	4.02e-13	68.9
MS.gene066807.t1	AT1G69580	49.231	65	32	1	168	231	34	98	4.09e-13	68.9
MS.gene066807.t1	AT3G12730	34.746	118	73	3	149	264	3	118	4.34e-13	67.4
MS.gene066807.t1	AT1G25550	48.000	75	38	1	154	227	195	269	6.25e-13	68.2
MS.gene066807.t1	AT2G20570	56.897	58	25	0	167	224	152	209	7.66e-13	68.6
MS.gene066807.t1	AT2G40260	40.594	101	53	3	134	233	51	145	9.81e-13	68.2
MS.gene066807.t1	AT1G14600	61.538	52	19	1	170	220	25	76	1.27e-12	66.6
MS.gene066807.t1	AT5G06800	58.182	55	22	1	168	221	192	246	1.99e-12	66.2
MS.gene066807.t1	AT5G06800	51.471	68	30	2	168	234	192	257	2.08e-12	67.0
MS.gene066807.t1	AT5G06800	58.182	55	22	1	168	221	192	246	3.04e-12	66.6
MS.gene066807.t1	AT4G04605	45.333	75	39	2	166	238	12	86	3.56e-12	64.7
MS.gene066807.t1	AT3G13040	53.571	56	25	1	167	221	240	295	4.68e-12	66.2
MS.gene066807.t1	AT3G13040	53.571	56	25	1	167	221	240	295	4.68e-12	66.2
MS.gene066807.t1	AT3G13040	53.571	56	25	1	167	221	240	295	4.68e-12	66.2
MS.gene066807.t1	AT2G20570	58.182	55	23	0	170	224	171	225	5.17e-12	65.9
MS.gene066807.t1	AT5G45580	39.080	87	52	1	157	242	12	98	7.36e-12	64.7
MS.gene066807.t1	AT2G20400	59.259	54	21	1	168	220	231	284	8.74e-12	65.1
MS.gene066807.t1	AT2G20400	59.259	54	21	1	168	220	231	284	8.88e-12	65.1
MS.gene066807.t1	AT4G18020	42.667	75	40	1	150	224	281	352	1.20e-11	65.1
MS.gene066807.t1	AT4G18020	42.667	75	40	1	150	224	281	352	1.20e-11	65.1
MS.gene066807.t1	AT4G18020	42.667	75	40	1	150	224	281	352	1.20e-11	65.1
MS.gene066807.t1	AT4G18020	42.667	75	40	1	150	224	281	352	1.20e-11	65.1
MS.gene066807.t1	AT4G18020	42.667	75	40	1	150	224	281	352	1.20e-11	65.1
MS.gene066807.t1	AT4G18020	42.667	75	40	1	150	224	281	352	1.20e-11	65.1
MS.gene066807.t1	AT4G18020	42.667	75	40	1	150	224	281	352	1.27e-11	65.1
MS.gene066807.t1	AT4G18020	42.667	75	40	1	150	224	281	352	1.27e-11	65.1
MS.gene066807.t1	AT4G18020	42.667	75	40	1	150	224	281	352	1.27e-11	65.1
MS.gene066807.t1	AT4G18020	42.667	75	40	1	150	224	281	352	1.27e-11	65.1
MS.gene066807.t1	AT4G18020	42.667	75	40	1	150	224	281	352	1.27e-11	65.1
MS.gene066807.t1	AT2G03500	50.000	62	30	1	167	227	232	293	1.62e-11	64.3
MS.gene066807.t1	AT2G38300	44.928	69	34	2	156	220	39	107	1.69e-11	63.9
MS.gene066807.t1	AT5G45580	40.741	81	47	1	163	242	2	82	1.93e-11	63.2
MS.gene066807.t1	AT2G42660	43.750	64	35	1	158	220	40	103	2.65e-11	62.8

Find 65 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTGCATCAACGATTTCTA+TGG	0.226165	4.2:-80839957	MS.gene066807:CDS
CAAAATGATTCTCAAAATTA+TGG	0.243357	4.2:-80839810	MS.gene066807:CDS
ATGACAGGAATGTTGAGTTT+TGG	0.275913	4.2:+80840337	None:intergenic
ACATGTTGCTAGTCATTTAC+AGG	0.286115	4.2:-80839765	MS.gene066807:intron
ATCCTATCTTTACAACCTTA+TGG	0.297081	4.2:-80839476	MS.gene066807:CDS
CTCAAAATTATGGATGTTCC+TGG	0.317981	4.2:-80839800	MS.gene066807:CDS
CTTGAATTTGTTGGAAAGAA+AGG	0.326280	4.2:-80840434	MS.gene066807:CDS
AGAAATCGTTGATGCAATTC+TGG	0.327125	4.2:+80839961	None:intergenic
CTCTGATGCCTTGAATTTGT+TGG	0.328493	4.2:-80840443	MS.gene066807:CDS
CACATATTGGACTAAGCCTT+TGG	0.377509	4.2:-80840157	MS.gene066807:CDS
AAAGTGACTAACCTTGATAA+TGG	0.378712	4.2:+80840595	None:intergenic
CTGAATGGATTGATCAAAAT+CGG	0.378920	4.2:-80839540	MS.gene066807:CDS
AATTATGGATGTTCCTGGTT+TGG	0.386251	4.2:-80839795	MS.gene066807:CDS
ATTATGGATGTTCCTGGTTT+GGG	0.388266	4.2:-80839794	MS.gene066807:CDS
CTTTCTTTCCAACAAATTCA+AGG	0.396613	4.2:+80840435	None:intergenic
ATCAGAGGCTGTATTACATT+TGG	0.397305	4.2:+80840459	None:intergenic
GGTTCAAGAAAATAGAAAAC+GGG	0.418887	4.2:-80840046	MS.gene066807:CDS
AATGTCGTTTGCAGAAATAT+AGG	0.426550	4.2:-80839636	MS.gene066807:intron
AATAAAGAATATGAAATTGT+TGG	0.433266	4.2:-80840078	MS.gene066807:CDS
TGTAATCGATTCCATTATCA+AGG	0.450836	4.2:-80840606	MS.gene066807:intron
ATATGAAATTGTTGGGAGCT+TGG	0.451488	4.2:-80840070	MS.gene066807:CDS
CGAAGAAGGCCCGTTTGTCT+TGG	0.454157	4.2:-80839987	MS.gene066807:CDS
AATAAATTCTTACTAATGAC+AGG	0.461511	4.2:+80840322	None:intergenic
ATAAAGAATATGAAATTGTT+GGG	0.463791	4.2:-80840077	MS.gene066807:CDS
AATGTGGTGCTTGCACATAT+TGG	0.466383	4.2:-80840170	MS.gene066807:CDS
TTGAGACCAGCAGGGAATGA+TGG	0.467536	4.2:+80840681	None:intergenic
TTCTGGCGACCAAGACAAAC+GGG	0.468019	4.2:+80839978	None:intergenic
AAGTCATAAGCATCCATGTT+TGG	0.471983	4.2:+80840376	None:intergenic
GGCCATAAGGTTGTAAAGAT+AGG	0.473529	4.2:+80839474	None:intergenic
ACGATGACTTTCTAGCTGAA+TGG	0.481545	4.2:-80839516	MS.gene066807:CDS
GATGTTCCTGGTTTGGGTGT+AGG	0.487511	4.2:-80839788	MS.gene066807:CDS
GAATGGATTGACTGTGGGGC+GGG	0.487587	4.2:-80839499	MS.gene066807:CDS
AAAAGTAACACATGCGAAGA+AGG	0.492090	4.2:-80840001	MS.gene066807:CDS
TAGCTGAATGGATTGACTGT+GGG	0.492334	4.2:-80839504	MS.gene066807:CDS
CACTTTCCTCAACTCTATTG+AGG	0.492388	4.2:-80840634	MS.gene066807:CDS
GCAATAACCTTGAGACCAGC+AGG	0.494358	4.2:+80840672	None:intergenic
TAAACGGCATCTAGGCCATA+AGG	0.496018	4.2:+80839461	None:intergenic
ATTCTGGCGACCAAGACAAA+CGG	0.509916	4.2:+80839977	None:intergenic
AGGAAAGAATCATACTATCA+AGG	0.510494	4.2:+80839917	None:intergenic
TAGGATTCATTTGAAGAGAT+CGG	0.515087	4.2:-80839617	MS.gene066807:CDS
TGGTTCAAGAAAATAGAAAA+CGG	0.517102	4.2:-80840047	MS.gene066807:CDS
CTATGGGCTGTCAATCAACT+AGG	0.518108	4.2:-80839940	MS.gene066807:CDS
GTGATAGCTCTCAAGCTGAA+TGG	0.519299	4.2:-80839555	MS.gene066807:CDS
ATCATTCCCTGCTGGTCTCA+AGG	0.520371	4.2:-80840679	MS.gene066807:CDS
TTGAACTCTTCTTCAACCAA+AGG	0.521847	4.2:+80840141	None:intergenic
GACAACCCATCATTCCCTGC+TGG	0.539365	4.2:-80840687	MS.gene066807:CDS
TGAGACCAGCAGGGAATGAT+GGG	0.539790	4.2:+80840682	None:intergenic
ATTGCATCAACGATTTCTAT+GGG	0.552524	4.2:-80839956	MS.gene066807:CDS
AGAACTTTCTGCGGAATCAA+CGG	0.562052	4.2:+80840209	None:intergenic
CTAGCTGAATGGATTGACTG+TGG	0.582882	4.2:-80839505	MS.gene066807:CDS
GAAAGTGATGTCATGATCAA+TGG	0.586165	4.2:+80840650	None:intergenic
CAACAAATTCAAGGCATCAG+AGG	0.592040	4.2:+80840444	None:intergenic
CTTCATAATAGAACTTTCTG+CGG	0.598305	4.2:+80840200	None:intergenic
TGATGCTCATATGCCAAACA+TGG	0.604750	4.2:-80840389	MS.gene066807:CDS
ACATGTCCTACACCCAAACC+AGG	0.611300	4.2:+80839782	None:intergenic
AGCTGAATGGATTGACTGTG+GGG	0.620097	4.2:-80839503	MS.gene066807:CDS
ACATCTCCTCAATAGAGTTG+AGG	0.625514	4.2:+80840628	None:intergenic
TGAAATTGTTGGGAGCTTGG+TGG	0.628025	4.2:-80840067	MS.gene066807:CDS
AAGAAGAGTTCAAGATCATG+TGG	0.629722	4.2:-80840131	MS.gene066807:CDS
ATTATGAAGTCTATTGAATG+TGG	0.654435	4.2:-80840186	MS.gene066807:CDS
TGAATGGATTGACTGTGGGG+CGG	0.657142	4.2:-80839500	MS.gene066807:CDS
CGGCTGATGAAACAAAACAC+GGG	0.673622	4.2:-80839597	MS.gene066807:CDS
CAATAACCTTGAGACCAGCA+GGG	0.699486	4.2:+80840673	None:intergenic
TCGGCTGATGAAACAAAACA+CGG	0.707340	4.2:-80839598	MS.gene066807:CDS
GTTCAAGAAAATAGAAAACG+GGG	0.742093	4.2:-80840045	MS.gene066807:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAAAGAATATGAAATTGT+TGG	-	chr4.2:80840095-80840114	MS.gene066807:CDS	15.0%
!!	ATAAAGAATATGAAATTGTT+GGG	-	chr4.2:80840096-80840115	MS.gene066807:CDS	15.0%
!!!	ATAATTTTGAGAATCATTTT+GGG	+	chr4.2:80840364-80840383	None:intergenic	15.0%
!!	AATAAATTCTTACTAATGAC+AGG	+	chr4.2:80839854-80839873	None:intergenic	20.0%
!!	CAAAATGATTCTCAAAATTA+TGG	-	chr4.2:80840363-80840382	MS.gene066807:CDS	20.0%
!!!	CATAATTTTGAGAATCATTT+TGG	+	chr4.2:80840365-80840384	None:intergenic	20.0%
!!!	TTATTTCTTTTTTGTAGTGA+TGG	-	chr4.2:80839941-80839960	MS.gene066807:CDS	20.0%
!	ACAAAGCAAAGAAAAGAATT+AGG	+	chr4.2:80840279-80840298	None:intergenic	25.0%
!	ATTATGAAGTCTATTGAATG+TGG	-	chr4.2:80839987-80840006	MS.gene066807:CDS	25.0%
!	TGGTTCAAGAAAATAGAAAA+CGG	-	chr4.2:80840126-80840145	MS.gene066807:CDS	25.0%
!!	TCAAACCTTTTCTTAAAACA+TGG	+	chr4.2:80840070-80840089	None:intergenic	25.0%
	AAAGTGACTAACCTTGATAA+TGG	+	chr4.2:80839581-80839600	None:intergenic	30.0%
	AATTGCATCAACGATTTCTA+TGG	-	chr4.2:80840216-80840235	MS.gene066807:CDS	30.0%
	AGGAAAGAATCATACTATCA+AGG	+	chr4.2:80840259-80840278	None:intergenic	30.0%
	ATCCTATCTTTACAACCTTA+TGG	-	chr4.2:80840697-80840716	MS.gene066807:CDS	30.0%
	ATTGCATCAACGATTTCTAT+GGG	-	chr4.2:80840217-80840236	MS.gene066807:CDS	30.0%
	CTTCATAATAGAACTTTCTG+CGG	+	chr4.2:80839976-80839995	None:intergenic	30.0%
	CTTTCTTTCCAACAAATTCA+AGG	+	chr4.2:80839741-80839760	None:intergenic	30.0%
	GGTTCAAGAAAATAGAAAAC+GGG	-	chr4.2:80840127-80840146	MS.gene066807:CDS	30.0%
	GTTCAAGAAAATAGAAAACG+GGG	-	chr4.2:80840128-80840147	MS.gene066807:CDS	30.0%
	TAGGATTCATTTGAAGAGAT+CGG	-	chr4.2:80840556-80840575	MS.gene066807:intron	30.0%
!	AATGTCGTTTGCAGAAATAT+AGG	-	chr4.2:80840537-80840556	MS.gene066807:intron	30.0%
!	TGTAATCGATTCCATTATCA+AGG	-	chr4.2:80839567-80839586	MS.gene066807:CDS	30.0%
!!	CTGAATGGATTGATCAAAAT+CGG	-	chr4.2:80840633-80840652	MS.gene066807:CDS	30.0%
!!	CTTGAATTTGTTGGAAAGAA+AGG	-	chr4.2:80839739-80839758	MS.gene066807:intron	30.0%
!!	GTCATTTACAGGTTTGTTTT+CGG	-	chr4.2:80840419-80840438	MS.gene066807:CDS	30.0%
	AAAAGTAACACATGCGAAGA+AGG	-	chr4.2:80840172-80840191	MS.gene066807:CDS	35.0%
	AAGAAGAGTTCAAGATCATG+TGG	-	chr4.2:80840042-80840061	MS.gene066807:CDS	35.0%
	AAGTCATAAGCATCCATGTT+TGG	+	chr4.2:80839800-80839819	None:intergenic	35.0%
	AATTATGGATGTTCCTGGTT+TGG	-	chr4.2:80840378-80840397	MS.gene066807:CDS	35.0%
	ACATGTTGCTAGTCATTTAC+AGG	-	chr4.2:80840408-80840427	MS.gene066807:CDS	35.0%
	ACGGGAAGAAAAAAACTAAG+TGG	-	chr4.2:80840594-80840613	MS.gene066807:intron	35.0%
	AGAAATCGTTGATGCAATTC+TGG	+	chr4.2:80840215-80840234	None:intergenic	35.0%
	ATATGAAATTGTTGGGAGCT+TGG	-	chr4.2:80840103-80840122	MS.gene066807:CDS	35.0%
	ATCAGAGGCTGTATTACATT+TGG	+	chr4.2:80839717-80839736	None:intergenic	35.0%
	ATTATGGATGTTCCTGGTTT+GGG	-	chr4.2:80840379-80840398	MS.gene066807:CDS	35.0%
	CTCAAAATTATGGATGTTCC+TGG	-	chr4.2:80840373-80840392	MS.gene066807:CDS	35.0%
	GAAAGTGATGTCATGATCAA+TGG	+	chr4.2:80839526-80839545	None:intergenic	35.0%
	TTGAACTCTTCTTCAACCAA+AGG	+	chr4.2:80840035-80840054	None:intergenic	35.0%
!	ATGACAGGAATGTTGAGTTT+TGG	+	chr4.2:80839839-80839858	None:intergenic	35.0%
!	TGACAGGAATGTTGAGTTTT+GGG	+	chr4.2:80839838-80839857	None:intergenic	35.0%
!!	TTTACAGGTTTGTTTTCGGA+AGG	-	chr4.2:80840423-80840442	MS.gene066807:CDS	35.0%
!!!	TGAGTTTTGGGGTAACATTT+TGG	+	chr4.2:80839826-80839845	None:intergenic	35.0%
!!!	TGGCACCATGTTTTAAGAAA+AGG	-	chr4.2:80840062-80840081	MS.gene066807:CDS	35.0%
	AATGTGGTGCTTGCACATAT+TGG	-	chr4.2:80840003-80840022	MS.gene066807:CDS	40.0%
	ACATCTCCTCAATAGAGTTG+AGG	+	chr4.2:80839548-80839567	None:intergenic	40.0%
	AGAACTTTCTGCGGAATCAA+CGG	+	chr4.2:80839967-80839986	None:intergenic	40.0%
	CACATATTGGACTAAGCCTT+TGG	-	chr4.2:80840016-80840035	MS.gene066807:CDS	40.0%
	CACTTTCCTCAACTCTATTG+AGG	-	chr4.2:80839539-80839558	MS.gene066807:CDS	40.0%
	CTCTGATGCCTTGAATTTGT+TGG	-	chr4.2:80839730-80839749	MS.gene066807:intron	40.0%
	GGCCATAAGGTTGTAAAGAT+AGG	+	chr4.2:80840702-80840721	None:intergenic	40.0%
	TCGGCTGATGAAACAAAACA+CGG	-	chr4.2:80840575-80840594	MS.gene066807:intron	40.0%
	TGATGCTCATATGCCAAACA+TGG	-	chr4.2:80839784-80839803	MS.gene066807:CDS	40.0%
!	CAACAAATTCAAGGCATCAG+AGG	+	chr4.2:80839732-80839751	None:intergenic	40.0%
!	GACAGGAATGTTGAGTTTTG+GGG	+	chr4.2:80839837-80839856	None:intergenic	40.0%
!!	ACGATGACTTTCTAGCTGAA+TGG	-	chr4.2:80840657-80840676	MS.gene066807:CDS	40.0%
!!	TAGCTGAATGGATTGACTGT+GGG	-	chr4.2:80840669-80840688	MS.gene066807:CDS	40.0%
!!!	CTTCGCATGTGTTACTTTTG+AGG	+	chr4.2:80840171-80840190	None:intergenic	40.0%
	ATTCTGGCGACCAAGACAAA+CGG	+	chr4.2:80840199-80840218	None:intergenic	45.0%
	CAATAACCTTGAGACCAGCA+GGG	+	chr4.2:80839503-80839522	None:intergenic	45.0%
	CGGCTGATGAAACAAAACAC+GGG	-	chr4.2:80840576-80840595	MS.gene066807:intron	45.0%
	GTGATAGCTCTCAAGCTGAA+TGG	-	chr4.2:80840618-80840637	MS.gene066807:CDS	45.0%
	TGAAATTGTTGGGAGCTTGG+TGG	-	chr4.2:80840106-80840125	MS.gene066807:CDS	45.0%
!	CTATGGGCTGTCAATCAACT+AGG	-	chr4.2:80840233-80840252	MS.gene066807:intron	45.0%
!!	AGCTGAATGGATTGACTGTG+GGG	-	chr4.2:80840670-80840689	MS.gene066807:CDS	45.0%
!!	CTAGCTGAATGGATTGACTG+TGG	-	chr4.2:80840668-80840687	MS.gene066807:CDS	45.0%
	ACATGTCCTACACCCAAACC+AGG	+	chr4.2:80840394-80840413	None:intergenic	50.0%
	GCAATAACCTTGAGACCAGC+AGG	+	chr4.2:80839504-80839523	None:intergenic	50.0%
	TGAGACCAGCAGGGAATGAT+GGG	+	chr4.2:80839494-80839513	None:intergenic	50.0%
	TTCTGGCGACCAAGACAAAC+GGG	+	chr4.2:80840198-80840217	None:intergenic	50.0%
	TTGAGACCAGCAGGGAATGA+TGG	+	chr4.2:80839495-80839514	None:intergenic	50.0%
!	ATCATTCCCTGCTGGTCTCA+AGG	-	chr4.2:80839494-80839513	MS.gene066807:CDS	50.0%
!!	GATGTTCCTGGTTTGGGTGT+AGG	-	chr4.2:80840385-80840404	MS.gene066807:CDS	50.0%
!!	TGAATGGATTGACTGTGGGG+CGG	-	chr4.2:80840673-80840692	MS.gene066807:CDS	50.0%
	CGAAGAAGGCCCGTTTGTCT+TGG	-	chr4.2:80840186-80840205	MS.gene066807:CDS	55.0%
	GACAACCCATCATTCCCTGC+TGG	-	chr4.2:80839486-80839505	MS.gene066807:CDS	55.0%
!!	GAATGGATTGACTGTGGGGC+GGG	-	chr4.2:80840674-80840693	MS.gene066807:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	80839471	80840724	80839471	ID=MS.gene066807
chr4.2	mRNA	80839471	80840724	80839471	ID=MS.gene066807.t1;Parent=MS.gene066807
chr4.2	exon	80840607	80840724	80840607	ID=MS.gene066807.t1.exon1;Parent=MS.gene066807.t1
chr4.2	CDS	80840607	80840724	80840607	ID=cds.MS.gene066807.t1;Parent=MS.gene066807.t1
chr4.2	exon	80840335	80840484	80840335	ID=MS.gene066807.t1.exon2;Parent=MS.gene066807.t1
chr4.2	CDS	80840335	80840484	80840335	ID=cds.MS.gene066807.t1;Parent=MS.gene066807.t1
chr4.2	exon	80839932	80840237	80839932	ID=MS.gene066807.t1.exon3;Parent=MS.gene066807.t1
chr4.2	CDS	80839932	80840237	80839932	ID=cds.MS.gene066807.t1;Parent=MS.gene066807.t1
chr4.2	exon	80839766	80839842	80839766	ID=MS.gene066807.t1.exon4;Parent=MS.gene066807.t1
chr4.2	CDS	80839766	80839842	80839766	ID=cds.MS.gene066807.t1;Parent=MS.gene066807.t1
chr4.2	exon	80839471	80839644	80839471	ID=MS.gene066807.t1.exon5;Parent=MS.gene066807.t1
chr4.2	CDS	80839471	80839644	80839471	ID=cds.MS.gene066807.t1;Parent=MS.gene066807.t1

Gene Sequence

>MS.gene066807

ATGGCTGTTTTCGTTGACAACCCATCATTCCCTGCTGGTCTCAAGGTTATTGCCATTGATCATGACATCACTTTCCTCAACTCTATTGAGGAGATGTGTAATCGATTCCATTATCAAGGTTAGTCACTTTGTTAATTTCACTTTATTCAATTCTGAATTTTCTATCTATGTTCTTACTTTTATGTATTTTCATGTTTGTTTCTTCATGCTTATGTCTATTTTTATGTTTGTTTATTTCAGTTACCAAATGTAATACAGCCTCTGATGCCTTGAATTTGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTGATGCTCATATGCCAAACATGGATGCTTATGACTTTGTCCAAAATGTTACCCCAAAACTCAACATTCCTGTCATTAGTAAGAATTTATTCAACTTTACACAATTCACATGATTCATTCATATATATGTACTTAATAAGTGTGAACTGACATTATTATTATTTCTTTTTTGTAGTGATGGCCGTTGATTCCGCAGAAAGTTCTATTATGAAGTCTATTGAATGTGGTGCTTGCACATATTGGACTAAGCCTTTGGTTGAAGAAGAGTTCAAGATCATGTGGCACCATGTTTTAAGAAAAGGTTTGACTGAAAATAAAGAATATGAAATTGTTGGGAGCTTGGTGGTTCAAGAAAATAGAAAACGGGGTAGAGAAGATGATAATGCCTCAAAAGTAACACATGCGAAGAAGGCCCGTTTGTCTTGGTCGCCAGAATTGCATCAACGATTTCTATGGGCTGTCAATCAACTAGGCCTTGATAGTATGATTCTTTCCTAATTCTTTTCTTTGCTTTGTTTGTATTATTTAACAACTCAACTTATGTGCATTTTTATCTCACTTATTTTTCAGAAGCAAGACCCAAAATGATTCTCAAAATTATGGATGTTCCTGGTTTGGGTGTAGGACATGTTGCTAGTCATTTACAGGTTTGTTTTCGGAAGGAGTCTAATTAAATTATTAATAATATACTACATGTATTTTGAGTGATATTAAATTGAAACTTATAGAGTACTATTGTTAAAATTGTGATTGCAATGTCGTTTGCAGAAATATAGGATTCATTTGAAGAGATCGGCTGATGAAACAAAACACGGGAAGAAAAAAACTAAGTGGAGTGATAGCTCTCAAGCTGAATGGATTGATCAAAATCGGAACGATGACTTTCTAGCTGAATGGATTGACTGTGGGGCGGGATCCTATCTTTACAACCTTATGGCCTAG

Protein sequence

>MS.gene066807.t1

MAVFVDNPSFPAGLKVIAIDHDITFLNSIEEMCNRFHYQVTKCNTASDALNLLERKDCFDVMLIDAHMPNMDAYDFVQNVTPKLNIPVIMMAVDSAESSIMKSIECGACTYWTKPLVEEEFKIMWHHVLRKGLTENKEYEIVGSLVVQENRKRGREDDNASKVTHAKKARLSWSPELHQRFLWAVNQLGLDKARPKMILKIMDVPGLGVGHVASHLQKYRIHLKRSADETKHGKKKTKWSDSSQAEWIDQNRNDDFLAEWIDCGAGSYLYNLMA