AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067193

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067193.t1 MTR_1g035570 51.986 277 95 3 158 432 163 403 1.91e-86 270
MS.gene067193.t1 MTR_1g035570 43.511 131 63 1 1 120 167 297 2.54e-25 107
MS.gene067193.t1 MTR_1g035490 52.256 266 106 5 161 425 85 330 1.60e-84 262
MS.gene067193.t1 MTR_1g035490 35.106 282 140 9 1 279 86 327 3.00e-36 136
MS.gene067193.t1 MTR_1g035620 48.529 272 108 12 161 422 159 408 7.27e-64 211
MS.gene067193.t1 MTR_1g035620 37.162 296 134 14 1 285 160 414 7.28e-39 145
MS.gene067193.t1 MTR_1g035640 42.308 312 133 10 157 424 157 465 4.15e-58 197
MS.gene067193.t1 MTR_1g035640 53.254 169 67 3 2 160 163 329 2.35e-44 161
MS.gene067193.t1 MTR_6g061070 39.604 303 164 12 137 423 171 470 9.65e-50 176
MS.gene067193.t1 MTR_6g061070 33.333 279 148 13 10 279 220 469 5.22e-28 115
MS.gene067193.t1 MTR_1g050845 48.879 223 91 10 175 390 158 364 3.17e-49 179
MS.gene067193.t1 MTR_1g050845 50.000 152 59 6 12 158 165 304 5.21e-32 129
MS.gene067193.t1 MTR_5g044670 28.571 245 133 8 76 287 74 309 2.56e-17 83.2
MS.gene067193.t1 MTR_5g044670 36.842 114 69 2 12 124 206 317 3.54e-14 73.6
MS.gene067193.t1 MTR_1g108780 27.350 351 200 16 110 422 123 456 2.02e-16 81.6
MS.gene067193.t1 MTR_8g103940 26.708 322 194 9 128 422 187 493 2.73e-16 81.3
MS.gene067193.t1 MTR_1g035520 38.596 114 65 4 314 423 271 383 5.45e-14 73.6
MS.gene067193.t1 MTR_1g035540 45.588 68 33 2 219 286 8 71 4.87e-12 63.5
MS.gene067193.t1 MTR_1g035540 53.191 47 22 0 75 121 31 77 1.17e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 77 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTGTTCTATTGTTCTTTA+AGG 0.153282 1.2:+20717252 MS.gene067193:CDS
AAACTCTCTGGAATTGAAAA+TGG 0.167608 1.2:+20717044 MS.gene067193:CDS
GTATTTGATGTTTCAATTGT+AGG 0.254587 1.2:+20717213 MS.gene067193:CDS
TCAAAGAGAGAAGAATTTAA+AGG 0.254601 1.2:+20716380 MS.gene067193:CDS
CCAAAGAGAAAAGGATTTAA+AGG 0.262203 1.2:+20715124 MS.gene067193:CDS
GCACACTTGAACAAATTTAA+AGG 0.264956 1.2:+20715973 MS.gene067193:CDS
ATCCCAATTACCAGGTTATA+AGG 0.281923 1.2:+20716331 MS.gene067193:intron
ACGATAGTATTCATGGTTAT+AGG 0.290213 1.2:+20717100 MS.gene067193:CDS
AAGCTTGGTTGCTCCCTTGA+TGG 0.305403 1.2:-20716186 None:intergenic
GAGCTTGATTGCTCCCTTGA+TGG 0.312398 1.2:-20714931 None:intergenic
AAGAATTTAAAGGAACTTCT+AGG 0.316735 1.2:+20716390 MS.gene067193:CDS
AATTATTACGATAGTATTCA+TGG 0.354510 1.2:+20717093 MS.gene067193:CDS
AACTCTCTGGAATTGAAAAT+GGG 0.368127 1.2:+20717045 MS.gene067193:CDS
CACACTTGAACAAATTTAAA+GGG 0.370401 1.2:+20715974 MS.gene067193:CDS
CTCTCATTAACAACTTCAAC+TGG 0.412647 1.2:-20715736 None:intergenic
TTTCAAGAAGATTATGTGTT+GGG 0.419256 1.2:-20715162 None:intergenic
GGGATTGCAAATTTAAGAGT+TGG 0.438060 1.2:+20715994 MS.gene067193:CDS
CCAAAGGAGTTTATGAAAAG+AGG 0.438669 1.2:+20717012 MS.gene067193:CDS
ATACTTTCAAGATGATTTGT+TGG 0.445012 1.2:-20716419 None:intergenic
AAAGAATGCAAAGTGGAGAT+TGG 0.446068 1.2:+20717150 MS.gene067193:CDS
AGTCACAAGAGATCTTCTAT+TGG 0.450938 1.2:-20716058 None:intergenic
AGGAAGTCATGAAAACTCTC+TGG 0.451275 1.2:+20717032 MS.gene067193:CDS
AACAATAGAACAAGCAGCTA+AGG 0.460018 1.2:-20717244 None:intergenic
TACTTTCAAGATGATTTGTT+GGG 0.461255 1.2:-20716418 None:intergenic
GTTATAGGTTCTCTGAAGGT+TGG 0.464034 1.2:+20717115 MS.gene067193:CDS
GATATTAATTTCTTTAAGCT+TGG 0.469147 1.2:-20716201 None:intergenic
CCGTTACTATTATTCCATCA+AGG 0.471250 1.2:+20714917 MS.gene067193:CDS
AGAAGATCTCTTGTGACTAC+TGG 0.477196 1.2:+20716063 MS.gene067193:CDS
AAGGATTTAAAGGAACTTCA+AGG 0.480588 1.2:+20715134 MS.gene067193:CDS
CACACTTGAACAAATTCAAA+GGG 0.482195 1.2:+20714719 MS.gene067193:CDS
CAGAGGAGGCATGGTTAAGA+AGG 0.489632 1.2:+20714498 None:intergenic
CCGTTACTATTACTCCATCA+AGG 0.499776 1.2:+20716172 MS.gene067193:CDS
GACTTACCATCTTGCAGCCA+TGG 0.504164 1.2:-20714582 None:intergenic
GGTGATAATCCAAAGAGAAA+AGG 0.509436 1.2:+20715115 MS.gene067193:CDS
GGGATTGCAAACTTAAGAGT+TGG 0.509708 1.2:+20714739 MS.gene067193:CDS
CAATTACCAGGTTATAAAGA+AGG 0.517705 1.2:+20715079 MS.gene067193:intron
TGCAAATTTAAGAGTTGGTG+AGG 0.518069 1.2:+20715999 MS.gene067193:CDS
CGTTACTATTATTCCATCAA+GGG 0.530233 1.2:+20714918 MS.gene067193:CDS
TGAATTATCACCACAAGAAG+AGG 0.531434 1.2:-20716360 None:intergenic
ACAATAGAACAAGCAGCTAA+GGG 0.537935 1.2:-20717243 None:intergenic
TTACCAACATATTGCTGCCA+TGG 0.540173 1.2:-20715810 None:intergenic
CATGGTTATAGGTTCTCTGA+AGG 0.540492 1.2:+20717111 MS.gene067193:CDS
GCACACTTGAACAAATTCAA+AGG 0.541236 1.2:+20714718 MS.gene067193:CDS
CAGCCATGGGCGTAAGATTG+TGG 0.541599 1.2:-20714568 None:intergenic
CTTTCAAGAAGATTATGTGT+TGG 0.544833 1.2:-20715163 None:intergenic
TCAAAGAAATTAAAATCGCA+AGG 0.552843 1.2:+20714952 MS.gene067193:CDS
ATAGTAACGGTAAATGAAAG+TGG 0.556798 1.2:-20714904 None:intergenic
TGGATTATCACCACAAGAAG+AGG 0.557132 1.2:-20715104 None:intergenic
GATCTCTTGTGACTACTGGT+TGG 0.563285 1.2:+20716067 MS.gene067193:CDS
CAATTACCAGGTTATAAGGA+AGG 0.565649 1.2:+20716335 MS.gene067193:intron
TTAAAGAAATTAATATCGCA+AGG 0.569842 1.2:+20716207 MS.gene067193:CDS
CAGTGGAGGCATGATTAAGA+AGG 0.573799 1.2:+20715699 MS.gene067193:CDS
ATAGTAACGGTGAATGAAAG+TGG 0.574816 1.2:-20716159 None:intergenic
TTGTCAAAATCTTACGTCCA+TGG 0.576626 1.2:+20715793 MS.gene067193:CDS
TGTGTTTCTCCAGATTCAGA+AGG 0.578280 1.2:+20716832 MS.gene067193:intron
ACTTACCATCTTGCAGCCAT+GGG 0.578802 1.2:-20714581 None:intergenic
CGTCCATGGCAGCAATATGT+TGG 0.579724 1.2:+20715807 MS.gene067193:CDS
TTACGCCCATGGCTGCAAGA+TGG 0.581402 1.2:+20714576 MS.gene067193:CDS
AATTACCAGGTTATAAAGAA+GGG 0.583013 1.2:+20715080 MS.gene067193:intron
CTCTCTGGAATTGAAAATGG+GGG 0.588733 1.2:+20717047 MS.gene067193:CDS
ATATTTGCAGAATGTTCCAA+AGG 0.598638 1.2:+20716996 MS.gene067193:intron
CGTTACTATTACTCCATCAA+GGG 0.606445 1.2:+20716173 MS.gene067193:CDS
TTGCCACAATCTTACGCCCA+TGG 0.607348 1.2:+20714565 MS.gene067193:CDS
ACTCTCTGGAATTGAAAATG+GGG 0.608150 1.2:+20717046 MS.gene067193:CDS
TACTTTGCAGGTAGGAACAG+TGG 0.609109 1.2:+20715682 MS.gene067193:intron
CCTTGATGGAATAATAGTAA+CGG 0.610637 1.2:-20714917 None:intergenic
ATTCATAAAAGAATGCAAAG+TGG 0.613826 1.2:+20717143 MS.gene067193:CDS
TGAAGACATGACTATGAAAG+TGG 0.619803 1.2:+20714762 MS.gene067193:CDS
AATTACCAGGTTATAAGGAA+GGG 0.622190 1.2:+20716336 MS.gene067193:intron
AAAGAAGGGACCTCTTCTTG+TGG 0.632268 1.2:+20715094 MS.gene067193:CDS
AAGGAAGGGACCTCTTCTTG+TGG 0.640604 1.2:+20716350 MS.gene067193:CDS
CAATAGAACAAGCAGCTAAG+GGG 0.645203 1.2:-20717242 None:intergenic
AAAACGGGATTCTCAACTGG+TGG 0.653085 1.2:-20714532 None:intergenic
TGATGTGTATTCAAAATACC+CGG 0.654603 1.2:+20716888 MS.gene067193:CDS
CCTTGATGGAGTAATAGTAA+CGG 0.655922 1.2:-20716172 None:intergenic
TCTCTGGAATTGAAAATGGG+GGG 0.663804 1.2:+20717048 MS.gene067193:CDS
TTTGCAGGTAGGAACAGTGG+AGG 0.682532 1.2:+20715685 MS.gene067193:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTAATATTTAATCAATTAT+TGG - chr1.2:20715242-20715261 None:intergenic 10.0%
!! ATATTTAAAAAATGACTAAT+AGG - chr1.2:20715506-20715525 None:intergenic 10.0%
!!! ATCATTTTATATTTATTTTG+GGG - chr1.2:20715444-20715463 None:intergenic 10.0%
!!! CTATCATTTTATATTTATTT+TGG - chr1.2:20715446-20715465 None:intergenic 10.0%
!!! TTTTTTTTAAATAAACCAAA+AGG + chr1.2:20715395-20715414 MS.gene067193:intron 10.0%
!! AATCAATTATTGATAAATTC+AGG - chr1.2:20716484-20716503 None:intergenic 15.0%
!! AATCAATTATTGGTAAATTA+AGG - chr1.2:20715232-20715251 None:intergenic 15.0%
!! ACATTATTTCAAAATCATTA+GGG - chr1.2:20716578-20716597 None:intergenic 15.0%
!! ACGATTAATTACATAAAATA+TGG + chr1.2:20716912-20716931 MS.gene067193:intron 15.0%
!! ATCAATTATTGATAAATTCA+GGG - chr1.2:20716483-20716502 None:intergenic 15.0%
!! CATGATATATAAATAAATGT+AGG + chr1.2:20715553-20715572 MS.gene067193:intron 15.0%
!! GATCTTTAATAAATAAAATG+TGG + chr1.2:20714651-20714670 MS.gene067193:intron 15.0%
!! TACATTATTTCAAAATCATT+AGG - chr1.2:20716579-20716598 None:intergenic 15.0%
!!! ACTTATCAATAATTTGAAAT+AGG - chr1.2:20716687-20716706 None:intergenic 15.0%
!!! AGCATTTTTAATAACAAAAA+AGG - chr1.2:20714983-20715002 None:intergenic 15.0%
!!! TTTTATTTATTAAAGATCAG+TGG - chr1.2:20714649-20714668 None:intergenic 15.0%
!! AAAATGATAGAAACAAAAGT+TGG + chr1.2:20715456-20715475 MS.gene067193:intron 20.0%
!! AAATGATAGAAACAAAAGTT+GGG + chr1.2:20715457-20715476 MS.gene067193:intron 20.0%
!! AACAAATTATTAGAGATTAG+TGG - chr1.2:20715908-20715927 None:intergenic 20.0%
!! AATTATTACGATAGTATTCA+TGG + chr1.2:20717093-20717112 MS.gene067193:CDS 20.0%
!! CAAAAATATAACTTACAAGT+TGG + chr1.2:20714847-20714866 MS.gene067193:CDS 20.0%
!! GACAATGTAACATAAAAAAA+AGG - chr1.2:20715778-20715797 None:intergenic 20.0%
!! GATATTAATTTCTTTAAGCT+TGG - chr1.2:20716204-20716223 None:intergenic 20.0%
!! TATTAACTAATGCCAAAATT+AGG - chr1.2:20714626-20714645 None:intergenic 20.0%
!! TCAAATTATTGATAAGTTCA+AGG + chr1.2:20716690-20716709 MS.gene067193:intron 20.0%
!! TTAAAGAAATTAATATCGCA+AGG + chr1.2:20716207-20716226 MS.gene067193:CDS 20.0%
!!! AATTAGTAGACATTTTTAGA+TGG - chr1.2:20715841-20715860 None:intergenic 20.0%
!!! CAACTTGTAAGTTATATTTT+TGG - chr1.2:20714849-20714868 None:intergenic 20.0%
!!! CTAAAAATGTCTACTAATTT+TGG + chr1.2:20715842-20715861 MS.gene067193:intron 20.0%
!!! GATTAGTGGATTTTTATTTT+TGG - chr1.2:20715894-20715913 None:intergenic 20.0%
!!! GTTTTTTGTTCTTTCTTATT+TGG + chr1.2:20716229-20716248 MS.gene067193:intron 20.0%
!!! TTTTAAATAAACCAAAAGGA+TGG + chr1.2:20715399-20715418 MS.gene067193:intron 20.0%
!!! TTTTATGTAATTAATCGTAC+CGG - chr1.2:20716910-20716929 None:intergenic 20.0%
! AAGAATTTAAAGGAACTTCT+AGG + chr1.2:20716390-20716409 MS.gene067193:CDS 25.0%
! AATGATAGAAACAAAAGTTG+GGG + chr1.2:20715458-20715477 MS.gene067193:intron 25.0%
! AATTACCAGGTTATAAAGAA+GGG + chr1.2:20715080-20715099 MS.gene067193:intron 25.0%
! ATTCATAAAAGAATGCAAAG+TGG + chr1.2:20717143-20717162 MS.gene067193:CDS 25.0%
! ATTTCCTCATTATTCTCAAA+AGG - chr1.2:20715620-20715639 None:intergenic 25.0%
! CACACTTGAACAAATTTAAA+GGG + chr1.2:20715974-20715993 MS.gene067193:CDS 25.0%
! GTATTTGATGTTTCAATTGT+AGG + chr1.2:20717213-20717232 MS.gene067193:CDS 25.0%
! TCAAAGAAATTAAAATCGCA+AGG + chr1.2:20714952-20714971 MS.gene067193:CDS 25.0%
! TCAAAGAGAGAAGAATTTAA+AGG + chr1.2:20716380-20716399 MS.gene067193:CDS 25.0%
! TGGCAATGTAACATAAAAAA+CGG - chr1.2:20714551-20714570 None:intergenic 25.0%
! TTCTTTATAACCTGGTAATT+GGG - chr1.2:20715080-20715099 None:intergenic 25.0%
! TTTATGTAATTAATCGTACC+GGG - chr1.2:20716909-20716928 None:intergenic 25.0%
! TTTCAAGAAGATTATGTGTT+GGG - chr1.2:20715165-20715184 None:intergenic 25.0%
!! ACATCAATCTTGAATGTTTT+AGG - chr1.2:20716874-20716893 None:intergenic 25.0%
!! ATACTTTCAAGATGATTTGT+TGG - chr1.2:20716422-20716441 None:intergenic 25.0%
!! CTAAAAATGTCTCCTAATTT+TGG + chr1.2:20714611-20714630 MS.gene067193:intron 25.0%
!!! AAATCCTTTTGAGAATAATG+AGG + chr1.2:20715613-20715632 MS.gene067193:intron 25.0%
!!! TACTTTCAAGATGATTTGTT+GGG - chr1.2:20716421-20716440 None:intergenic 25.0%
AAGGATTTAAAGGAACTTCA+AGG + chr1.2:20715134-20715153 MS.gene067193:CDS 30.0%
AATTACCAGGTTATAAGGAA+GGG + chr1.2:20716336-20716355 MS.gene067193:intron 30.0%
ACGATAGTATTCATGGTTAT+AGG + chr1.2:20717100-20717119 MS.gene067193:CDS 30.0%
ATAGTAACGGTAAATGAAAG+TGG - chr1.2:20714907-20714926 None:intergenic 30.0%
ATATTTGCAGAATGTTCCAA+AGG + chr1.2:20716996-20717015 MS.gene067193:intron 30.0%
CAATTACCAGGTTATAAAGA+AGG + chr1.2:20715079-20715098 MS.gene067193:intron 30.0%
CACACTTGAACAAATTCAAA+GGG + chr1.2:20714719-20714738 MS.gene067193:CDS 30.0%
CCAAAGAGAAAAGGATTTAA+AGG + chr1.2:20715124-20715143 MS.gene067193:CDS 30.0%
CCTTGATGGAATAATAGTAA+CGG - chr1.2:20714920-20714939 None:intergenic 30.0%
CGTTACTATTATTCCATCAA+GGG + chr1.2:20714918-20714937 MS.gene067193:CDS 30.0%
CTGCAAAGTAATTCTCAAAA+GGG - chr1.2:20715672-20715691 None:intergenic 30.0%
CTTCTTTATAACCTGGTAAT+TGG - chr1.2:20715081-20715100 None:intergenic 30.0%
CTTTCAAGAAGATTATGTGT+TGG - chr1.2:20715166-20715185 None:intergenic 30.0%
GCACACTTGAACAAATTTAA+AGG + chr1.2:20715973-20715992 MS.gene067193:CDS 30.0%
GGCAATGTAACATAAAAAAC+GGG - chr1.2:20714550-20714569 None:intergenic 30.0%
TGATGTGTATTCAAAATACC+CGG + chr1.2:20716888-20716907 MS.gene067193:CDS 30.0%
TGTACATGATGAATTGAAAC+AGG + chr1.2:20715927-20715946 MS.gene067193:intron 30.0%
TGTGCTCTTGAAAAATTACT+TGG - chr1.2:20715958-20715977 None:intergenic 30.0%
TTCCTTATAACCTGGTAATT+GGG - chr1.2:20716336-20716355 None:intergenic 30.0%
! ATCATTTCAAAGTTCTAGCT+AGG + chr1.2:20716623-20716642 MS.gene067193:intron 30.0%
! CCTTTAAATCCTTTTCTCTT+TGG - chr1.2:20715127-20715146 None:intergenic 30.0%
! TCATTTCAAAGTTCTAGCTA+GGG + chr1.2:20716624-20716643 MS.gene067193:intron 30.0%
!! AAACTCTCTGGAATTGAAAA+TGG + chr1.2:20717044-20717063 MS.gene067193:CDS 30.0%
!! AACTCTCTGGAATTGAAAAT+GGG + chr1.2:20717045-20717064 MS.gene067193:CDS 30.0%
AAAGAATGCAAAGTGGAGAT+TGG + chr1.2:20717150-20717169 MS.gene067193:CDS 35.0%
AACAATAGAACAAGCAGCTA+AGG - chr1.2:20717247-20717266 None:intergenic 35.0%
ACAATAGAACAAGCAGCTAA+GGG - chr1.2:20717246-20717265 None:intergenic 35.0%
ACATTATCACCTTCTGAATC+TGG - chr1.2:20716844-20716863 None:intergenic 35.0%
AGTCACAAGAGATCTTCTAT+TGG - chr1.2:20716061-20716080 None:intergenic 35.0%
ATAGTAACGGTGAATGAAAG+TGG - chr1.2:20716162-20716181 None:intergenic 35.0%
ATCCCAATTACCAGGTTATA+AGG + chr1.2:20715075-20715094 MS.gene067193:intron 35.0%
ATTCTTTGATCCCAATTACC+AGG + chr1.2:20716323-20716342 MS.gene067193:intron 35.0%
CAATTACCAGGTTATAAGGA+AGG + chr1.2:20716335-20716354 MS.gene067193:intron 35.0%
CCAAAGGAGTTTATGAAAAG+AGG + chr1.2:20717012-20717031 MS.gene067193:CDS 35.0%
CCGTTACTATTATTCCATCA+AGG + chr1.2:20714917-20714936 MS.gene067193:CDS 35.0%
CCTGCAAAGTAATTCTCAAA+AGG - chr1.2:20715673-20715692 None:intergenic 35.0%
CCTTGATGGAGTAATAGTAA+CGG - chr1.2:20716175-20716194 None:intergenic 35.0%
CGTTACTATTACTCCATCAA+GGG + chr1.2:20716173-20716192 MS.gene067193:CDS 35.0%
CTCTCATTAACAACTTCAAC+TGG - chr1.2:20715739-20715758 None:intergenic 35.0%
CTTCCTTATAACCTGGTAAT+TGG - chr1.2:20716337-20716356 None:intergenic 35.0%
GCACACTTGAACAAATTCAA+AGG + chr1.2:20714718-20714737 MS.gene067193:CDS 35.0%
GGGATCAAAGAAAAAGAAGT+TGG - chr1.2:20715060-20715079 None:intergenic 35.0%
GGGATTGCAAATTTAAGAGT+TGG + chr1.2:20715994-20716013 MS.gene067193:CDS 35.0%
GGTGATAATCCAAAGAGAAA+AGG + chr1.2:20715115-20715134 MS.gene067193:CDS 35.0%
TAAAAAACGGGATTCTCAAC+TGG - chr1.2:20714538-20714557 None:intergenic 35.0%
TAGAAACAAAAGTTGGGGAA+AGG + chr1.2:20715463-20715482 MS.gene067193:intron 35.0%
TCACCTGTTTCAATTCATCA+TGG - chr1.2:20714678-20714697 None:intergenic 35.0%
TGAAGACATGACTATGAAAG+TGG + chr1.2:20714762-20714781 MS.gene067193:CDS 35.0%
TGAATTATCACCACAAGAAG+AGG - chr1.2:20716363-20716382 None:intergenic 35.0%
TGGTTAAGAAGGCTAAAAAG+TGG + chr1.2:20714509-20714528 MS.gene067193:CDS 35.0%
TTGAGAATTACTTTGCAGGT+AGG + chr1.2:20715674-20715693 MS.gene067193:intron 35.0%
TTGTCAAAATCTTACGTCCA+TGG + chr1.2:20715793-20715812 MS.gene067193:CDS 35.0%
TTTCTTTGATCCCAATTACC+AGG + chr1.2:20715067-20715086 MS.gene067193:intron 35.0%
! CCTCTTTTCATAAACTCCTT+TGG - chr1.2:20717015-20717034 None:intergenic 35.0%
!! ACTCTCTGGAATTGAAAATG+GGG + chr1.2:20717046-20717065 MS.gene067193:CDS 35.0%
!! CCTTTTGAGAATTACTTTGC+AGG + chr1.2:20715670-20715689 MS.gene067193:intron 35.0%
!! TGCAAATTTAAGAGTTGGTG+AGG + chr1.2:20715999-20716018 MS.gene067193:CDS 35.0%
!!! AGAAGATCTGTTTTGACTAC+TGG + chr1.2:20714808-20714827 MS.gene067193:CDS 35.0%
AACCAAAAGGATGGTAGCAT+AGG + chr1.2:20715408-20715427 MS.gene067193:intron 40.0%
AGAAGATCTCTTGTGACTAC+TGG + chr1.2:20716063-20716082 MS.gene067193:CDS 40.0%
AGGAAGTCATGAAAACTCTC+TGG + chr1.2:20717032-20717051 MS.gene067193:CDS 40.0%
CAATAGAACAAGCAGCTAAG+GGG - chr1.2:20717245-20717264 None:intergenic 40.0%
CATGGTTATAGGTTCTCTGA+AGG + chr1.2:20717111-20717130 MS.gene067193:CDS 40.0%
CCGTTACTATTACTCCATCA+AGG + chr1.2:20716172-20716191 MS.gene067193:CDS 40.0%
GGGATTGCAAACTTAAGAGT+TGG + chr1.2:20714739-20714758 MS.gene067193:CDS 40.0%
GGTCCATGATGAATTGAAAC+AGG + chr1.2:20714672-20714691 MS.gene067193:intron 40.0%
TGGATTATCACCACAAGAAG+AGG - chr1.2:20715107-20715126 None:intergenic 40.0%
TGTGTTTCTCCAGATTCAGA+AGG + chr1.2:20716832-20716851 MS.gene067193:intron 40.0%
TTACCAACATATTGCTGCCA+TGG - chr1.2:20715813-20715832 None:intergenic 40.0%
! GTTATAGGTTCTCTGAAGGT+TGG + chr1.2:20717115-20717134 MS.gene067193:CDS 40.0%
! TCAAAGTTCTAGCTAGGGTA+GGG + chr1.2:20716629-20716648 MS.gene067193:intron 40.0%
! TTCAAAGTTCTAGCTAGGGT+AGG + chr1.2:20716628-20716647 MS.gene067193:intron 40.0%
!! CTCTCTGGAATTGAAAATGG+GGG + chr1.2:20717047-20717066 MS.gene067193:CDS 40.0%
!! TCTCTGGAATTGAAAATGGG+GGG + chr1.2:20717048-20717067 MS.gene067193:CDS 40.0%
!!! GATCTGTTTTGACTACTGGT+TGG + chr1.2:20714812-20714831 MS.gene067193:CDS 40.0%
AAAACGGGATTCTCAACTGG+TGG - chr1.2:20714535-20714554 None:intergenic 45.0%
AAAGAAGGGACCTCTTCTTG+TGG + chr1.2:20715094-20715113 MS.gene067193:CDS 45.0%
ACTTACCATCTTGCAGCCAT+GGG - chr1.2:20714584-20714603 None:intergenic 45.0%
GAGGTCCCTTCTTTATAACC+TGG - chr1.2:20715088-20715107 None:intergenic 45.0%
GATCTCTTGTGACTACTGGT+TGG + chr1.2:20716067-20716086 MS.gene067193:CDS 45.0%
TACTTTGCAGGTAGGAACAG+TGG + chr1.2:20715682-20715701 MS.gene067193:intron 45.0%
! CACCTATGCTACCATCCTTT+TGG - chr1.2:20715413-20715432 None:intergenic 45.0%
! CAGTGGAGGCATGATTAAGA+AGG + chr1.2:20715699-20715718 MS.gene067193:CDS 45.0%
!! ATATAAATTTATTTAAGAAA+AGG - chr1.2:20716775-20716794 None:intergenic 5.0%
!!! AAACTTTTTTTTTTTTAATT+TGG + chr1.2:20715204-20715223 MS.gene067193:intron 5.0%
!!! TATCATTTTATATTTATTTT+GGG - chr1.2:20715445-20715464 None:intergenic 5.0%
AAGCTTGGTTGCTCCCTTGA+TGG - chr1.2:20716189-20716208 None:intergenic 50.0%
AAGGAAGGGACCTCTTCTTG+TGG + chr1.2:20716350-20716369 MS.gene067193:CDS 50.0%
CGTCCATGGCAGCAATATGT+TGG + chr1.2:20715807-20715826 MS.gene067193:CDS 50.0%
GACTTACCATCTTGCAGCCA+TGG - chr1.2:20714585-20714604 None:intergenic 50.0%
GAGCTTGATTGCTCCCTTGA+TGG - chr1.2:20714934-20714953 None:intergenic 50.0%
GAGGTCCCTTCCTTATAACC+TGG - chr1.2:20716344-20716363 None:intergenic 50.0%
TGAAAATGGGGGGACGATCA+TGG + chr1.2:20717058-20717077 MS.gene067193:CDS 50.0%
TTGCCACAATCTTACGCCCA+TGG + chr1.2:20714565-20714584 MS.gene067193:CDS 50.0%
TTTGCAGGTAGGAACAGTGG+AGG + chr1.2:20715685-20715704 MS.gene067193:intron 50.0%
! AGTTCTAGCTAGGGTAGGGT+AGG + chr1.2:20716633-20716652 MS.gene067193:intron 50.0%
! GTTCTAGCTAGGGTAGGGTA+GGG + chr1.2:20716634-20716653 MS.gene067193:intron 50.0%
CAGCCATGGGCGTAAGATTG+TGG - chr1.2:20714571-20714590 None:intergenic 55.0%
TTACGCCCATGGCTGCAAGA+TGG + chr1.2:20714576-20714595 MS.gene067193:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 20714508 20717272 20714508 ID=MS.gene067193
chr1.2 mRNA 20714508 20717272 20714508 ID=MS.gene067193.t1;Parent=MS.gene067193
chr1.2 exon 20714508 20714597 20714508 ID=MS.gene067193.t1.exon1;Parent=MS.gene067193.t1
chr1.2 CDS 20714508 20714597 20714508 ID=cds.MS.gene067193.t1;Parent=MS.gene067193.t1
chr1.2 exon 20714694 20714973 20714694 ID=MS.gene067193.t1.exon2;Parent=MS.gene067193.t1
chr1.2 CDS 20714694 20714973 20714694 ID=cds.MS.gene067193.t1;Parent=MS.gene067193.t1
chr1.2 exon 20715089 20715184 20715089 ID=MS.gene067193.t1.exon3;Parent=MS.gene067193.t1
chr1.2 CDS 20715089 20715184 20715089 ID=cds.MS.gene067193.t1;Parent=MS.gene067193.t1
chr1.2 exon 20715692 20715828 20715692 ID=MS.gene067193.t1.exon4;Parent=MS.gene067193.t1
chr1.2 CDS 20715692 20715828 20715692 ID=cds.MS.gene067193.t1;Parent=MS.gene067193.t1
chr1.2 exon 20715949 20716228 20715949 ID=MS.gene067193.t1.exon5;Parent=MS.gene067193.t1
chr1.2 CDS 20715949 20716228 20715949 ID=cds.MS.gene067193.t1;Parent=MS.gene067193.t1
chr1.2 exon 20716345 20716437 20716345 ID=MS.gene067193.t1.exon6;Parent=MS.gene067193.t1
chr1.2 CDS 20716345 20716437 20716345 ID=cds.MS.gene067193.t1;Parent=MS.gene067193.t1
chr1.2 exon 20716845 20716909 20716845 ID=MS.gene067193.t1.exon7;Parent=MS.gene067193.t1
chr1.2 CDS 20716845 20716909 20716845 ID=cds.MS.gene067193.t1;Parent=MS.gene067193.t1
chr1.2 exon 20717006 20717272 20717006 ID=MS.gene067193.t1.exon8;Parent=MS.gene067193.t1
chr1.2 CDS 20717006 20717272 20717006 ID=cds.MS.gene067193.t1;Parent=MS.gene067193.t1
Gene Sequence

>MS.gene067193

ATGGTTAAGAAGGCTAAAAAGTGGCCACCAGTTGAGAATCCCGTTTTTTATGTTACATTGCCACAATCTTACGCCCATGGCTGCAAGATGGTAAGTCTACTAGCTAAAAATGTCTCCTAATTTTGGCATTAGTTAATACCACTGATCTTTAATAAATAAAATGTGGTCCATGATGAATTGAAACAGGTGATACAAAGTGATTTTTCAAGAGCACACTTGAACAAATTCAAAGGGATTGCAAACTTAAGAGTTGGTGAAGACATGACTATGAAAGTGGATGTGATGTTTGAAGATAAGTATAGAAGATCTGTTTTGACTACTGGTTGGAAGTCATTTAACCAAAAATATAACTTACAAGTTGGTGATAAATGTAAATTTGTGATGACTCAAAGAGAACCACTTTCATTTACCGTTACTATTATTCCATCAAGGGAGCAATCAAGCTCAAAGAAATTAAAATCGCAAGGTTTTTCCTTTTTTGTTATTAAAAATGCTTTTATTTGATATTTGAATTCACTAGTTTTTTTTGTACTTTTGTACTTTTATTTTCCAACTTCTTTTTCTTTGATCCCAATTACCAGGTTATAAAGAAGGGACCTCTTCTTGTGGTGATAATCCAAAGAGAAAAGGATTTAAAGGAACTTCAAGGAGCTGCCCAACACATAATCTTCTTGAAAGTATTCTTTTAACTTCTCTAAACTTTTTTTTTTTTAATTTGGTTCCTTAATTTACCAATAATTGATTAAATATTAGTTGTTTATTGTATATTTAAACTAAAATATTGAATTTTTATATATCACTATTTTAATAAATCAAAAAATTTAATTATTTTAATGAATTTTTCATCATTTGATTACTATTTGAATAAAATGAAAATGTTGAACTTTTTTTTTTTAAATAAACCAAAAGGATGGTAGCATAGGTGCTAGTCAACCCCAAAATAAATATAAAATGATAGAAACAAAAGTTGGGGAAAGGTGTTTTGAATAAATTTTCCTATTAGTCATTTTTTAAATATGATATGATATGATTTAATTTTTTTTATCATGATATATAAATAAATGTAGGCTTGTTTCTAGTAAAATTACTTTTTCTAATACGTTGCAAATCCTTTTGAGAATAATGAGGAAATTTTTAATGATGAATGACAATAATGAGAATCCCTTTTGAGAATTACTTTGCAGGTAGGAACAGTGGAGGCATGATTAAGAAGGCTAAAAATTGCTCACCAGTTGAAGTTGTTAATGAGAGTGACAATAATGAGAATCCTTTTTTTTATGTTACATTGTCAAAATCTTACGTCCATGGCAGCAATATGTTGGTAAGTTTACCATCTAAAAATGTCTACTAATTTTGGCTTTAATTAACACTAATATATTGTTTCCAAAAATAAAAATCCACTAATCTCTAATAATTTGTTGTACATGATGAATTGAAACAGGCGATACCAAGTAATTTTTCAAGAGCACACTTGAACAAATTTAAAGGGATTGCAAATTTAAGAGTTGGTGAGGACAAGACTATTGAAGTGAATGTGACATTTGAAGAAGCCAATAGAAGATCTCTTGTGACTACTGGTTGGAAATCATTTAACCAAAAATATAACTTACAAGTTGGTGATAAATGTAAATTTGTGATGACTCAAAGAGAACCACTTTCATTCACCGTTACTATTACTCCATCAAGGGAGCAACCAAGCTTAAAGAAATTAATATCGCAAGGTTTTTTGTTCTTTCTTATTTGGTTTTATGTTATTAAAAATGCTTTTATTTGATATTTGAATTCACTAGTTTTTTTCTTCTTTTTCAACTTCTTATTCTTTGATCCCAATTACCAGGTTATAAGGAAGGGACCTCTTCTTGTGGTGATAATTCAAAGAGAGAAGAATTTAAAGGAACTTCTAGGAGCTGCCCAACAAATCATCTTGAAAGTATTCTTTTAACTTATCTTAAATTTTATTTTTTAATTTGATCCCTGAATTTATCAATAATTGATTAAGTCTTTGAACTTAAATGTTTTTAGTTTAGTGTATATTTAAACTTAAATTTTAAATTATTTATGATTATGCCCTAATGATTTTGAAATAATGTAAGTTTTTTAATTGTGTATTTACAAATCATTTCAAAGTTCTAGCTAGGGTAGGGTAGGGTTCGATTAATTTTTTTAATTAACTCAACCTATTTCAAATTATTGATAAGTTCAAGGACATAATTTAAATTTTTTAGTTCTAAGAAATATTTAAAAATCTTTAAATAATTTGTAGACCTTTTCTTAAATAAATTTATATATTTTTTTGAAGAAGAAATAAATTTATATATTAACATTGTGTTTCTCCAGATTCAGAAGGTGATAATGTGAAGAAACCTAAAACATTCAAGATTGATGTGTATTCAAAATACCCGGTACGATTAATTACATAAAATATGGTTACTATGTTAATATATAGATAGAGATGTTAGAGCTACATATATACATAATATTTTTGTTTATATTTGCAGAATGTTCCAAAGGAGTTTATGAAAAGAGGAAGTCATGAAAACTCTCTGGAATTGAAAATGGGGGGACGATCATGGTTTGTAAAAGTGAATTATTACGATAGTATTCATGGTTATAGGTTCTCTGAAGGTTGGAGACAATTCATAAAAGAATGCAAAGTGGAGATTGGAGATACTCTTCTCTTTAAGTTGATTGATGAGAGCAAGTTTGTATTTGATGTTTCAATTGTAGGAAAGAACCCCTTAGCTGCTTGTTCTATTGTTCTTTAA
Protein sequence

>MS.gene067193.t1

MVKKAKKWPPVENPVFYVTLPQSYAHGCKMVIQSDFSRAHLNKFKGIANLRVGEDMTMKVDVMFEDKYRRSVLTTGWKSFNQKYNLQVGDKCKFVMTQREPLSFTVTIIPSREQSSSKKLKSQGYKEGTSSCGDNPKRKGFKGTSRSCPTHNLLESRNSGGMIKKAKNCSPVEVVNESDNNENPFFYVTLSKSYVHGSNMLAIPSNFSRAHLNKFKGIANLRVGEDKTIEVNVTFEEANRRSLVTTGWKSFNQKYNLQVGDKCKFVMTQREPLSFTVTITPSREQPSLKKLISQGYKEGTSSCGDNSKREEFKGTSRSCPTNHLENSEGDNVKKPKTFKIDVYSKYPNVPKEFMKRGSHENSLELKMGGRSWFVKVNYYDSIHGYRFSEGWRQFIKECKVEIGDTLLFKLIDESKFVFDVSIVGKNPLAACSIVL