Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067201.t1 | XP_024630392.1 | 88.8 | 160 | 17 | 1 | 1 | 160 | 147 | 305 | 1.60E-73 | 285.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067201.t1 | A0A396JU70 | 88.6 | 158 | 17 | 1 | 1 | 158 | 147 | 303 | 9.8e-73 | 282.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene067201.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067201.t1 | MTR_4g119990 | 58.824 | 170 | 52 | 4 | 5 | 157 | 155 | 323 | 2.99e-58 | 186 |
| MS.gene067201.t1 | MTR_6g043890 | 59.091 | 154 | 45 | 4 | 1 | 137 | 145 | 297 | 9.85e-51 | 165 |
| MS.gene067201.t1 | MTR_1g035460 | 32.432 | 111 | 70 | 3 | 44 | 154 | 190 | 295 | 5.91e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGAAAATAATTTGAAATT+TGG | 0.140450 | 1.2:-20621622 | MS.gene067201:CDS |
| ATTTATCTTTGTCAACAAAA+AGG | 0.196489 | 1.2:+20621707 | None:intergenic |
| CTCTATCAATGTATGACTTT+TGG | 0.220204 | 1.2:+20622099 | None:intergenic |
| AAGTAGTTGTGATGATATTA+AGG | 0.299219 | 1.2:-20622195 | MS.gene067201:CDS |
| ATATAATTACCTTCAAAATA+AGG | 0.343429 | 1.2:+20621511 | None:intergenic |
| GAGAAAATAACAACTTGAAA+TGG | 0.395512 | 1.2:+20621556 | None:intergenic |
| TTTCTCTTTGGAAAAGGACT+TGG | 0.399841 | 1.2:-20621539 | MS.gene067201:CDS |
| TGTGTCTTAACAAATTCATT+TGG | 0.404885 | 1.2:+20621763 | None:intergenic |
| TGAATTTGTTAAGACACATA+TGG | 0.433535 | 1.2:-20621758 | MS.gene067201:CDS |
| CAACCATTGTTCACATGCTC+AGG | 0.447561 | 1.2:+20622313 | None:intergenic |
| ATACATTGATAGAGATCGCT+TGG | 0.539899 | 1.2:-20622090 | MS.gene067201:intron |
| AATCTGCACTAACTTGTGGT+TGG | 0.573287 | 1.2:-20621657 | MS.gene067201:CDS |
| TAAGACACATATGGACATGA+TGG | 0.584937 | 1.2:-20621749 | MS.gene067201:CDS |
| AAACAATCTGCACTAACTTG+TGG | 0.589005 | 1.2:-20621661 | MS.gene067201:CDS |
| ACACATATGGACATGATGGA+AGG | 0.627742 | 1.2:-20621745 | MS.gene067201:CDS |
| AAGCCTGAGCATGTGAACAA+TGG | 0.703416 | 1.2:-20622316 | MS.gene067201:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGATTTATGAAAAATTTA+TGG | - | chr1.2:20622302-20622321 | MS.gene067201:intron | 10.0% |
| !! | ATATAATTACCTTCAAAATA+AGG | + | chr1.2:20622270-20622289 | None:intergenic | 15.0% |
| !!! | ATTTCAAATTATTTTCTTCA+CGG | + | chr1.2:20622155-20622174 | None:intergenic | 15.0% |
| !!! | GAAGAAAATAATTTGAAATT+TGG | - | chr1.2:20622156-20622175 | MS.gene067201:CDS | 15.0% |
| !!! | TATTTTGAAGGTAATTATAT+TGG | - | chr1.2:20622270-20622289 | MS.gene067201:intron | 15.0% |
| !!! | TTTTTGCTACTAATTTTTTT+TGG | - | chr1.2:20621537-20621556 | MS.gene067201:CDS | 15.0% |
| !! | AACTATATGTGTGTATTTAT+AGG | - | chr1.2:20621987-20622006 | MS.gene067201:intron | 20.0% |
| !! | ATTTATCTTTGTCAACAAAA+AGG | + | chr1.2:20622074-20622093 | None:intergenic | 20.0% |
| !! | CCAAATTATTATACCATAAT+TGG | - | chr1.2:20621813-20621832 | MS.gene067201:intron | 20.0% |
| !! | CCAATTATGGTATAATAATT+TGG | + | chr1.2:20621816-20621835 | None:intergenic | 20.0% |
| !! | TTTATCTTTGTCAACAAAAA+GGG | + | chr1.2:20622073-20622092 | None:intergenic | 20.0% |
| !!! | CTTTAAAAATACCTACATAA+TGG | - | chr1.2:20621863-20621882 | MS.gene067201:intron | 20.0% |
| !!! | TGAATTCTTATTTTTTTAGC+AGG | - | chr1.2:20621560-20621579 | MS.gene067201:CDS | 20.0% |
| !!! | TTTTTGTTGACAAAGATAAA+TGG | - | chr1.2:20622073-20622092 | MS.gene067201:intron | 20.0% |
| ! | AAGTAGTTGTGATGATATTA+AGG | - | chr1.2:20621583-20621602 | MS.gene067201:CDS | 25.0% |
| ! | AATATAGCAAAGTCCAATTA+TGG | + | chr1.2:20621829-20621848 | None:intergenic | 25.0% |
| ! | CAATATAATAATTCATGTGC+TGG | - | chr1.2:20622346-20622365 | MS.gene067201:CDS | 25.0% |
| ! | GAGAAAATAACAACTTGAAA+TGG | + | chr1.2:20622225-20622244 | None:intergenic | 25.0% |
| ! | TGTGTCTTAACAAATTCATT+TGG | + | chr1.2:20622018-20622037 | None:intergenic | 25.0% |
| !!! | TCAAGTTGTTATTTTCTCTT+TGG | - | chr1.2:20622227-20622246 | MS.gene067201:intron | 25.0% |
| !!! | TGAATTTGTTAAGACACATA+TGG | - | chr1.2:20622020-20622039 | MS.gene067201:intron | 25.0% |
| !!! | TGTGTTTTTGTGTTAAACAA+AGG | - | chr1.2:20622186-20622205 | MS.gene067201:CDS | 25.0% |
| !!! | TGTTATTTTCTCTTTGGAAA+AGG | - | chr1.2:20622233-20622252 | MS.gene067201:intron | 25.0% |
| GAAAAAATTCGACTCTGATA+AGG | - | chr1.2:20621637-20621656 | MS.gene067201:CDS | 30.0% | |
| ! | CTCTATCAATGTATGACTTT+TGG | + | chr1.2:20621682-20621701 | None:intergenic | 30.0% |
| !!! | CGAATTTTTTCTTCACTTTC+TGG | + | chr1.2:20621628-20621647 | None:intergenic | 30.0% |
| !!! | TTGGAAACTCCTTATTTTGA+AGG | - | chr1.2:20622258-20622277 | MS.gene067201:intron | 30.0% |
| AAACAATCTGCACTAACTTG+TGG | - | chr1.2:20622117-20622136 | MS.gene067201:CDS | 35.0% | |
| ATACATTGATAGAGATCGCT+TGG | - | chr1.2:20621688-20621707 | MS.gene067201:CDS | 35.0% | |
| CCAATCACAATCCATTATGT+AGG | + | chr1.2:20621877-20621896 | None:intergenic | 35.0% | |
| TAAGACACATATGGACATGA+TGG | - | chr1.2:20622029-20622048 | MS.gene067201:intron | 35.0% | |
| ! | TTTCTCTTTGGAAAAGGACT+TGG | - | chr1.2:20622239-20622258 | MS.gene067201:intron | 35.0% |
| !! | CCTACATAATGGATTGTGAT+TGG | - | chr1.2:20621874-20621893 | MS.gene067201:intron | 35.0% |
| TCATGTGCTGGACTATTGTA+GGG | - | chr1.2:20622358-20622377 | MS.gene067201:CDS | 40.0% | |
| TTCATGTGCTGGACTATTGT+AGG | - | chr1.2:20622357-20622376 | MS.gene067201:CDS | 40.0% | |
| ! | AATCTGCACTAACTTGTGGT+TGG | - | chr1.2:20622121-20622140 | MS.gene067201:CDS | 40.0% |
| ! | ACACATATGGACATGATGGA+AGG | - | chr1.2:20622033-20622052 | MS.gene067201:intron | 40.0% |
| AAGCCTGAGCATGTGAACAA+TGG | - | chr1.2:20621462-20621481 | MS.gene067201:intron | 45.0% | |
| CAACCATTGTTCACATGCTC+AGG | + | chr1.2:20621468-20621487 | None:intergenic | 45.0% | |
| CTGAGCATGTGAACAATGGT+TGG | - | chr1.2:20621466-20621485 | MS.gene067201:intron | 45.0% | |
| GTGCTGGACTATTGTAGGGT+TGG | - | chr1.2:20622362-20622381 | MS.gene067201:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 20621411 | 20622389 | 20621411 | ID=MS.gene067201 |
| chr1.2 | mRNA | 20621411 | 20622389 | 20621411 | ID=MS.gene067201.t1;Parent=MS.gene067201 |
| chr1.2 | exon | 20622317 | 20622389 | 20622317 | ID=MS.gene067201.t1.exon1;Parent=MS.gene067201.t1 |
| chr1.2 | CDS | 20622317 | 20622389 | 20622317 | ID=cds.MS.gene067201.t1;Parent=MS.gene067201.t1 |
| chr1.2 | exon | 20622091 | 20622218 | 20622091 | ID=MS.gene067201.t1.exon2;Parent=MS.gene067201.t1 |
| chr1.2 | CDS | 20622091 | 20622218 | 20622091 | ID=cds.MS.gene067201.t1;Parent=MS.gene067201.t1 |
| chr1.2 | exon | 20621521 | 20621791 | 20621521 | ID=MS.gene067201.t1.exon3;Parent=MS.gene067201.t1 |
| chr1.2 | CDS | 20621521 | 20621791 | 20621521 | ID=cds.MS.gene067201.t1;Parent=MS.gene067201.t1 |
| chr1.2 | exon | 20621411 | 20621421 | 20621411 | ID=MS.gene067201.t1.exon4;Parent=MS.gene067201.t1 |
| chr1.2 | CDS | 20621411 | 20621421 | 20621411 | ID=cds.MS.gene067201.t1;Parent=MS.gene067201.t1 |
| Gene Sequence |
| Protein sequence |
