Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067207.t1 | KEH40802.1 | 91.2 | 80 | 7 | 0 | 1 | 80 | 304 | 383 | 1.00E-36 | 162.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067207.t1 | A0A072VG92 | 91.2 | 80 | 7 | 0 | 1 | 80 | 304 | 383 | 7.4e-37 | 162.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene067207.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067207.t1 | MTR_1g035520 | 91.250 | 80 | 7 | 0 | 1 | 80 | 304 | 383 | 6.84e-49 | 158 |
| MS.gene067207.t1 | MTR_6g061070 | 83.750 | 80 | 12 | 1 | 1 | 80 | 392 | 470 | 5.68e-43 | 144 |
| MS.gene067207.t1 | MTR_1g035490 | 48.718 | 78 | 36 | 3 | 4 | 80 | 254 | 328 | 6.53e-18 | 75.9 |
| MS.gene067207.t1 | MTR_1g035570 | 46.835 | 79 | 36 | 2 | 4 | 80 | 320 | 394 | 6.79e-17 | 73.6 |
| MS.gene067207.t1 | MTR_1g035620 | 42.466 | 73 | 40 | 1 | 7 | 79 | 338 | 408 | 4.07e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGACATAATATATTTAG+TGG | 0.334344 | 1.2:-20548101 | MS.gene067207:CDS |
| TATAATGAACATCTCGACTA+TGG | 0.355043 | 1.2:-20548026 | MS.gene067207:CDS |
| TAATATATTTAGTGGAAATT+CGG | 0.396970 | 1.2:-20548093 | MS.gene067207:CDS |
| ATTCGGTGGAGTTGAAAGTA+GGG | 0.471281 | 1.2:-20548076 | MS.gene067207:CDS |
| TGTGCAAAATCACGTGTTCA+AGG | 0.494647 | 1.2:-20547922 | MS.gene067207:CDS |
| TGAAAGTAGGGGAAGGAACA+TGG | 0.498346 | 1.2:-20548064 | MS.gene067207:CDS |
| AATTCGGTGGAGTTGAAAGT+AGG | 0.502201 | 1.2:-20548077 | MS.gene067207:CDS |
| AGAGATTGCAAAGTAAAGAC+TGG | 0.513081 | 1.2:-20547972 | MS.gene067207:CDS |
| GACTATGGATGGTTCACTAA+AGG | 0.536976 | 1.2:-20548011 | MS.gene067207:CDS |
| TATATTTAGTGGAAATTCGG+TGG | 0.544355 | 1.2:-20548090 | MS.gene067207:CDS |
| ATGGATGGTTCACTAAAGGC+TGG | 0.558556 | 1.2:-20548007 | MS.gene067207:CDS |
| ATGAACATCTCGACTATGGA+TGG | 0.566956 | 1.2:-20548022 | MS.gene067207:CDS |
| GGAAGGAACATGGTTTGTAG+AGG | 0.629163 | 1.2:-20548054 | MS.gene067207:CDS |
| TTCGGTGGAGTTGAAAGTAG+GGG | 0.634551 | 1.2:-20548075 | MS.gene067207:CDS |
| GTGGAGTTGAAAGTAGGGGA+AGG | 0.644645 | 1.2:-20548071 | MS.gene067207:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGACATAATATATTTAG+TGG | - | chr1.2:20547935-20547954 | MS.gene067207:CDS | 15.0% |
| !! | TAATATATTTAGTGGAAATT+CGG | - | chr1.2:20547943-20547962 | MS.gene067207:CDS | 15.0% |
| TATAATGAACATCTCGACTA+TGG | - | chr1.2:20548010-20548029 | MS.gene067207:CDS | 30.0% | |
| TATATTTAGTGGAAATTCGG+TGG | - | chr1.2:20547946-20547965 | MS.gene067207:CDS | 30.0% | |
| AGAGATTGCAAAGTAAAGAC+TGG | - | chr1.2:20548064-20548083 | MS.gene067207:CDS | 35.0% | |
| ! | TGTCTTTTTAACAGCTCACT+TGG | + | chr1.2:20547923-20547942 | None:intergenic | 35.0% |
| ATGAACATCTCGACTATGGA+TGG | - | chr1.2:20548014-20548033 | MS.gene067207:CDS | 40.0% | |
| GACTATGGATGGTTCACTAA+AGG | - | chr1.2:20548025-20548044 | MS.gene067207:CDS | 40.0% | |
| TGTGCAAAATCACGTGTTCA+AGG | - | chr1.2:20548114-20548133 | MS.gene067207:CDS | 40.0% | |
| ! | AATTCGGTGGAGTTGAAAGT+AGG | - | chr1.2:20547959-20547978 | MS.gene067207:CDS | 40.0% |
| ! | ATTCGGTGGAGTTGAAAGTA+GGG | - | chr1.2:20547960-20547979 | MS.gene067207:CDS | 40.0% |
| ATGGATGGTTCACTAAAGGC+TGG | - | chr1.2:20548029-20548048 | MS.gene067207:CDS | 45.0% | |
| TGAAAGTAGGGGAAGGAACA+TGG | - | chr1.2:20547972-20547991 | MS.gene067207:CDS | 45.0% | |
| TTCGGTGGAGTTGAAAGTAG+GGG | - | chr1.2:20547961-20547980 | MS.gene067207:CDS | 45.0% | |
| ! | GGAAGGAACATGGTTTGTAG+AGG | - | chr1.2:20547982-20548001 | MS.gene067207:CDS | 45.0% |
| GTGGAGTTGAAAGTAGGGGA+AGG | - | chr1.2:20547965-20547984 | MS.gene067207:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 20547908 | 20548150 | 20547908 | ID=MS.gene067207 |
| chr1.2 | mRNA | 20547908 | 20548150 | 20547908 | ID=MS.gene067207.t1;Parent=MS.gene067207 |
| chr1.2 | exon | 20547908 | 20548150 | 20547908 | ID=MS.gene067207.t1.exon1;Parent=MS.gene067207.t1 |
| chr1.2 | CDS | 20547908 | 20548150 | 20547908 | ID=cds.MS.gene067207.t1;Parent=MS.gene067207.t1 |
| Gene Sequence |
| Protein sequence |
