AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067248

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067248.t1 MTR_7g446520 56.376 298 105 3 58 350 30 307 6.00e-105 328
MS.gene067248.t1 MTR_7g446480 75.000 176 44 0 190 365 140 315 5.81e-87 280
MS.gene067248.t1 MTR_7g446510 74.432 176 45 0 190 365 155 330 3.80e-85 276
MS.gene067248.t1 MTR_7g446510 28.395 243 86 6 451 693 1 155 3.37e-17 84.3
MS.gene067248.t1 MTR_1g050570 76.744 172 40 0 717 888 1 172 6.06e-72 234
MS.gene067248.t1 MTR_7g028855 54.460 213 84 2 57 256 51 263 1.06e-69 239
MS.gene067248.t1 MTR_8g467420 78.462 130 28 0 37 166 2 131 8.27e-51 176
MS.gene067248.t1 MTR_1g070355 37.652 247 152 1 104 350 65 309 5.04e-49 183
MS.gene067248.t1 MTR_6g051425 33.462 260 169 3 123 379 27 285 1.29e-45 167
MS.gene067248.t1 MTR_5g076070 34.034 238 155 1 105 342 45 280 4.54e-42 155
MS.gene067248.t1 MTR_6g022070 36.207 174 111 0 410 583 1 174 8.24e-29 119
MS.gene067248.t1 MTR_0645s0010 39.370 127 74 1 488 614 1 124 4.35e-27 107
MS.gene067248.t1 MTR_4g035820 30.400 250 160 7 119 360 81 324 5.82e-23 105
MS.gene067248.t1 MTR_6g022080 29.070 172 122 0 104 275 76 247 1.17e-21 95.9
MS.gene067248.t1 MTR_0402s0040 32.558 215 120 8 80 289 6 200 1.54e-21 99.8
MS.gene067248.t1 MTR_0558s0020 26.820 261 164 7 117 355 40 295 3.30e-20 92.8
MS.gene067248.t1 MTR_8g468460 45.918 98 52 1 532 629 9 105 3.75e-20 87.0
MS.gene067248.t1 MTR_3g450850 27.848 237 153 7 119 342 53 284 1.11e-15 80.1
MS.gene067248.t1 MTR_1g008290 31.298 131 84 3 185 314 22 147 4.89e-14 70.9
MS.gene067248.t1 MTR_5g015480 32.258 186 111 7 53 234 2 176 1.67e-12 68.9
MS.gene067248.t1 MTR_0060s0090 39.241 79 48 0 116 194 59 137 3.42e-12 65.9
MS.gene067248.t1 MTR_6g090505 24.731 279 161 9 158 418 21 268 4.56e-12 69.3
MS.gene067248.t1 MTR_2g098880 35.514 107 67 2 114 219 62 167 5.06e-12 67.4
MS.gene067248.t1 MTR_2g098880 35.514 107 67 2 114 219 74 179 6.03e-12 67.4
MS.gene067248.t1 MTR_6g053140 25.668 187 122 3 38 224 35 204 7.25e-12 66.2
MS.gene067248.t1 MTR_8g446020 26.437 174 122 4 185 355 85 255 9.18e-12 66.6
MS.gene067248.t1 MTR_7g074790 30.723 166 94 3 115 280 12 156 2.30e-11 65.1
MS.gene067248.t1 MTR_2g101460 30.400 125 87 0 104 228 46 170 3.46e-11 64.3
MS.gene067248.t1 MTR_2g101460 30.400 125 87 0 104 228 36 160 4.66e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067248.t1 AT4G38180 31.194 670 428 11 56 708 21 674 1.40e-102 337
MS.gene067248.t1 AT4G15090 27.258 598 417 10 117 706 55 642 5.47e-81 279
MS.gene067248.t1 AT4G15090 27.258 598 417 10 117 706 55 642 5.47e-81 279
MS.gene067248.t1 AT4G15090 27.258 598 417 10 117 706 55 642 5.47e-81 279
MS.gene067248.t1 AT4G15090 27.258 598 417 10 117 706 55 642 5.47e-81 279
MS.gene067248.t1 AT4G15090 27.258 598 417 10 117 706 55 642 5.47e-81 279
MS.gene067248.t1 AT3G06250 27.994 618 388 13 110 719 187 755 4.25e-77 267
MS.gene067248.t1 AT3G06250 27.994 618 388 13 110 719 187 755 4.25e-77 267
MS.gene067248.t1 AT3G06250 27.994 618 388 13 110 719 187 755 4.25e-77 267
MS.gene067248.t1 AT3G22170 27.287 656 424 14 72 707 29 651 8.19e-72 254
MS.gene067248.t1 AT3G22170 27.287 656 424 14 72 707 29 651 8.19e-72 254
MS.gene067248.t1 AT5G18960 28.433 619 373 18 117 719 215 779 8.52e-72 253
MS.gene067248.t1 AT5G18960 28.433 619 373 18 117 719 215 779 8.52e-72 253
MS.gene067248.t1 AT1G76320 26.643 563 387 10 117 671 1 545 5.69e-65 233
MS.gene067248.t1 AT1G76320 26.643 563 387 10 117 671 1 545 5.82e-65 233
MS.gene067248.t1 AT1G76320 26.643 563 387 10 117 671 1 545 5.82e-65 233
MS.gene067248.t1 AT1G76320 26.643 563 387 10 117 671 1 545 5.90e-65 231
MS.gene067248.t1 AT1G80010 28.019 621 400 17 89 683 75 674 5.54e-63 227
MS.gene067248.t1 AT2G27110 26.452 620 407 14 58 670 2 579 2.25e-61 224
MS.gene067248.t1 AT2G27110 26.452 620 407 14 58 670 2 579 2.25e-61 224
MS.gene067248.t1 AT1G10240 26.813 593 392 13 119 689 54 626 6.94e-61 220
MS.gene067248.t1 AT1G10240 26.813 593 392 13 119 689 54 626 6.94e-61 220
MS.gene067248.t1 AT2G32250 24.795 609 393 11 117 719 1 550 5.13e-59 216
MS.gene067248.t1 AT2G32250 24.795 609 393 11 117 719 1 550 5.80e-59 216
MS.gene067248.t1 AT2G32250 24.795 609 393 11 117 719 42 591 5.90e-59 216
MS.gene067248.t1 AT2G32250 24.795 609 393 11 117 719 42 591 5.90e-59 216
MS.gene067248.t1 AT2G32250 24.795 609 393 11 117 719 42 591 6.86e-59 216
MS.gene067248.t1 AT2G32250 24.795 609 393 11 117 719 42 591 8.03e-59 216
MS.gene067248.t1 AT2G32250 24.795 609 393 11 117 719 42 591 8.03e-59 216
MS.gene067248.t1 AT2G32250 24.795 609 393 11 117 719 42 591 8.03e-59 216
MS.gene067248.t1 AT1G52520 28.055 581 387 11 117 683 89 652 5.06e-58 212
MS.gene067248.t1 AT4G19990 26.342 596 365 13 118 689 26 571 7.24e-58 213
MS.gene067248.t1 AT4G19990 26.342 596 365 13 118 689 26 571 7.33e-58 213
MS.gene067248.t1 AT4G19990 26.342 596 365 13 118 689 26 571 7.33e-58 213
MS.gene067248.t1 AT4G19990 26.342 596 365 13 118 689 40 585 8.14e-58 213
MS.gene067248.t1 AT4G19990 26.342 596 365 13 118 689 26 571 1.05e-57 213
MS.gene067248.t1 AT4G19990 26.342 596 365 13 118 689 26 571 1.05e-57 213
MS.gene067248.t1 AT2G27110 29.716 387 264 5 289 670 51 434 2.40e-55 204
MS.gene067248.t1 AT5G28530 25.297 589 404 13 119 685 61 635 9.71e-53 196
MS.gene067248.t1 AT4G38170 27.835 388 267 6 290 670 18 399 1.68e-52 193
MS.gene067248.t1 AT4G19990 26.046 526 327 10 176 689 39 514 2.99e-52 195
MS.gene067248.t1 AT5G28530 24.958 589 402 13 119 685 61 631 1.41e-51 193
MS.gene067248.t1 AT5G28530 24.958 589 402 13 119 685 61 631 1.41e-51 193
MS.gene067248.t1 AT5G28530 22.396 576 374 12 119 685 61 572 2.14e-35 144
MS.gene067248.t1 AT5G28530 22.049 576 372 12 119 685 61 568 1.82e-34 140
MS.gene067248.t1 AT3G07500 33.333 126 80 3 94 218 14 136 4.33e-11 63.9

Find 154 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGCATTCTTCTTCTTCTT+TGG 0.173135 8.1:-59163397 None:intergenic
GAAATTCTTAAACTTGAATT+TGG 0.176852 8.1:+59161468 MS.gene067248:CDS
TGTAAAGAAAACACCATTTC+TGG 0.203603 8.1:+59162442 MS.gene067248:CDS
ACCGCCATGAAGTCCATAAT+TGG 0.212290 8.1:-59163938 None:intergenic
TTGGCACAAGTCATGAATTT+TGG 0.213622 8.1:+59163988 MS.gene067248:CDS
CATTCACAGACCATTATATT+TGG 0.220424 8.1:+59162221 MS.gene067248:CDS
CCATCTGTTACAACTGCTTT+TGG 0.225965 8.1:-59162326 None:intergenic
TATTTACGTGATAAGTTCTT+TGG 0.228072 8.1:+59162608 MS.gene067248:CDS
GAATCATCAACTTCCTGTTC+TGG 0.232408 8.1:+59163961 MS.gene067248:CDS
GAAGAATGAAAAGTCTGTTT+TGG 0.246187 8.1:+59162081 MS.gene067248:CDS
CAGCCAATTTACCCCAATTA+TGG 0.247084 8.1:+59163925 MS.gene067248:CDS
CGGAGCAATTTGAGGAGTTT+TGG 0.252202 8.1:+59162501 MS.gene067248:CDS
ACACCATTTCTGGAGGGTTT+TGG 0.254714 8.1:+59162452 MS.gene067248:CDS
TGTACGTTACAAAGCCAAAA+AGG 0.274297 8.1:+59161710 MS.gene067248:CDS
AGTCCATAATTGGGGTAAAT+TGG 0.292438 8.1:-59163928 None:intergenic
AATCAGAACCTGTCATATAA+TGG 0.299188 8.1:+59161869 MS.gene067248:CDS
CTGTCATATAATGGGATATA+TGG 0.309391 8.1:+59161878 MS.gene067248:CDS
AGCACAATTTACAGAGATTT+CGG 0.314466 8.1:+59163822 MS.gene067248:CDS
GCCTTGCTTCACAATTTGTT+CGG 0.315678 8.1:-59161683 None:intergenic
AACTTCCTGTTCTGGGCTCT+TGG 0.321078 8.1:+59163969 MS.gene067248:CDS
TACCCCAATTATGGACTTCA+TGG 0.333754 8.1:+59163934 MS.gene067248:CDS
CGGAGTGAAGATGGCGCTGC+TGG 0.341163 8.1:-59160365 None:intergenic
CCATTAGGAAAGGTGATGTT+AGG 0.342221 8.1:+59161547 MS.gene067248:CDS
TATATTTGGCGTTGCGTTGT+TGG 0.345466 8.1:+59162235 MS.gene067248:CDS
CCTTGCTTCACAATTTGTTC+GGG 0.352426 8.1:-59161682 None:intergenic
TGATCACATACCAAAATGTT+TGG 0.362382 8.1:+59163090 MS.gene067248:CDS
ATGATTGAAGTTGCTAGATT+TGG 0.363762 8.1:+59163187 MS.gene067248:CDS
TTCGGAGACAGCTGATTTGC+CGG 0.364644 8.1:+59163840 MS.gene067248:CDS
GGAACACCACAAAGATTGTT+TGG 0.365076 8.1:+59161248 MS.gene067248:CDS
ATGAACTTGAAGAAAATGAT+TGG 0.365813 8.1:+59162543 MS.gene067248:CDS
ATTTAGGTGGGGTTAATTCA+TGG 0.368536 8.1:-59161770 None:intergenic
CTTGTGCTAACATTACCATT+AGG 0.373645 8.1:-59163775 None:intergenic
CAACGATTCCAATGGGGAAA+TGG 0.377917 8.1:+59163156 MS.gene067248:CDS
CGGGTCATAACCAAACATTT+TGG 0.385088 8.1:-59163100 None:intergenic
CCGCCATGAAGTCCATAATT+GGG 0.393287 8.1:-59163937 None:intergenic
CCTTTACTCTTCACAGGGAT+TGG 0.395830 8.1:-59163370 None:intergenic
ATCAGAACCTGTCATATAAT+GGG 0.403538 8.1:+59161870 MS.gene067248:CDS
GGTTGAACTGCAGTTGTTAC+CGG 0.408018 8.1:-59163859 None:intergenic
AATCATCAACTTCCTGTTCT+GGG 0.413052 8.1:+59163962 MS.gene067248:CDS
TTTCCAAAACCCTCCAGAAA+TGG 0.414871 8.1:-59162455 None:intergenic
CAACGCAACGCCAAATATAA+TGG 0.420340 8.1:-59162231 None:intergenic
GAGACAAGAAGCACTTATCT+AGG 0.423504 8.1:+59161628 MS.gene067248:CDS
TGGTGTATTTAGGGACATAA+TGG 0.425462 8.1:+59163261 MS.gene067248:CDS
GGGTGCAAGTGTGTAAATTT+AGG 0.429032 8.1:-59161786 None:intergenic
TTCTGAACAACGATTCCAAT+GGG 0.429337 8.1:+59163149 MS.gene067248:CDS
CATCTCATCGCTTATGTGCC+TGG 0.431687 8.1:+59162393 MS.gene067248:CDS
CGCGTTCTTATGCAGATGCC+AGG 0.434981 8.1:-59162411 None:intergenic
TGAGAAATTAACAGATGATC+AGG 0.435117 8.1:+59163339 MS.gene067248:CDS
AGAATTGGTATCTAACTCTA+TGG 0.436678 8.1:+59163632 MS.gene067248:CDS
GATCTCTGCTGTGTAAATCT+TGG 0.442124 8.1:-59162837 None:intergenic
CCTAACATCACCTTTCCTAA+TGG 0.442699 8.1:-59161547 None:intergenic
TTTGGGCTGATGGGAGTAGT+AGG 0.444063 8.1:+59162099 MS.gene067248:CDS
GAATACGATGTCAACATTGA+TGG 0.449029 8.1:+59162059 MS.gene067248:CDS
CATAAGCGATGAGATGCATC+AGG 0.456050 8.1:-59162386 None:intergenic
ACATGAACATGATGCAGAAT+TGG 0.463393 8.1:+59163617 MS.gene067248:CDS
TGAACTTGAAGAAAATGATT+GGG 0.463828 8.1:+59162544 MS.gene067248:CDS
AATGAGGGAAGCTATTAAAC+AGG 0.464184 8.1:+59162358 MS.gene067248:CDS
AGGCTTGGAGAGCGAGGTGG+TGG 0.470187 8.1:-59160389 None:intergenic
AAAGATGAAATTATTGAGGC+TGG 0.470276 8.1:+59162872 MS.gene067248:CDS
GGAGCACCTTTACTCTTCAC+AGG 0.470733 8.1:-59163376 None:intergenic
TAAATTGGCTGCATTTGCAT+TGG 0.473815 8.1:-59163913 None:intergenic
GTATTCTACTTCACTGGAAT+CGG 0.474179 8.1:-59161274 None:intergenic
ATCAGAATCCATTTCCCCAT+TGG 0.476697 8.1:-59163164 None:intergenic
AAGAATTTCATCAGAGGCAA+TGG 0.477228 8.1:-59161454 None:intergenic
ATCTGTGTATTCTACTTCAC+TGG 0.479751 8.1:-59161280 None:intergenic
GGGATATATGGTTGCTCAGA+AGG 0.480775 8.1:+59161890 MS.gene067248:CDS
TCAATCAACAATGCATTAGT+AGG 0.482447 8.1:+59161408 MS.gene067248:CDS
TTGTGCCAAGAGCCCAGAAC+AGG 0.484522 8.1:-59163974 None:intergenic
TGGCGCTGCTGGGTCGCACG+TGG 0.486275 8.1:-59160354 None:intergenic
GCAAGTGTGTAAATTTAGGT+GGG 0.497265 8.1:-59161782 None:intergenic
TCATACTGCAAGGCATTGTT+CGG 0.499503 8.1:+59163459 MS.gene067248:CDS
GGTGGTGGACGGAGTGAAGA+TGG 0.501161 8.1:-59160374 None:intergenic
TCAAGAGCAAAGAATGTGTA+GGG 0.501912 8.1:+59162906 MS.gene067248:CDS
TTGCGAAAATATCGTGAGAA+AGG 0.510344 8.1:+59162947 MS.gene067248:CDS
CACCTCGCTCTCCAAGCCTC+AGG 0.510859 8.1:+59160393 MS.gene067248:CDS
ATTTGCATTGGATTAATAGC+AGG 0.513071 8.1:-59163901 None:intergenic
CAAAACTCCTCAAATTGCTC+CGG 0.514523 8.1:-59162500 None:intergenic
GATGCTGAAGATGATGTTGA+TGG 0.520081 8.1:+59161339 MS.gene067248:CDS
GAAAGAAGAGGCAACGGAGT+GGG 0.522053 8.1:-59160314 None:intergenic
CGGCGTCCAAACAATCTTTG+TGG 0.524396 8.1:-59161254 None:intergenic
CATATATCCCATTATATGAC+AGG 0.525157 8.1:-59161877 None:intergenic
CAAGCAAAAGAAATATAAAG+AGG 0.527664 8.1:+59163798 MS.gene067248:CDS
AGTTAAAGATGAAATTATTG+AGG 0.530146 8.1:+59162868 MS.gene067248:CDS
CTTGATGCTCTTCAATCACA+TGG 0.535182 8.1:+59161846 MS.gene067248:CDS
TGCAAGTGTGTAAATTTAGG+TGG 0.535511 8.1:-59161783 None:intergenic
GTGATTGAAGAGCATCAAGT+TGG 0.536251 8.1:-59161842 None:intergenic
TGATTGAAGAGCATCAAGTT+GGG 0.536427 8.1:-59161841 None:intergenic
TATCGTTATGGAAGATGCAA+AGG 0.541216 8.1:+59161519 MS.gene067248:CDS
TGCATTAGTAGGTGAAAGGT+TGG 0.543116 8.1:+59161419 MS.gene067248:CDS
GAGAGAGAAAGAAGAGGCAA+CGG 0.543213 8.1:-59160320 None:intergenic
GCATTGGATTAATAGCAGGC+TGG 0.543520 8.1:-59163897 None:intergenic
GGAGTGAAGATGGCGCTGCT+GGG 0.543895 8.1:-59160364 None:intergenic
CCAATCCCTGTGAAGAGTAA+AGG 0.544969 8.1:+59163370 MS.gene067248:CDS
ATTTGGCGTTGCGTTGTTGG+AGG 0.548289 8.1:+59162238 MS.gene067248:CDS
AGAAAGAAGAGGCAACGGAG+TGG 0.548447 8.1:-59160315 None:intergenic
AAGGGTGGATACGCTGATGT+AGG 0.558445 8.1:+59161909 MS.gene067248:CDS
TTGGAGAGCGAGGTGGTGGA+CGG 0.559403 8.1:-59160385 None:intergenic
CCAAAAGCAGTTGTAACAGA+TGG 0.560492 8.1:+59162326 MS.gene067248:CDS
TAGAAAACGAACCTGAGGCT+TGG 0.561009 8.1:-59160404 None:intergenic
AATGTTTGGTTATGACCCGT+TGG 0.566267 8.1:+59163104 MS.gene067248:CDS
AAACAATCGAGTCATATAAG+TGG 0.567873 8.1:+59162264 MS.gene067248:CDS
GAGTTTGTGTGCAACAAGCA+AGG 0.575972 8.1:+59161600 MS.gene067248:CDS
GTAGTGTCTAAATTTGAAGA+GGG 0.581617 8.1:+59161741 MS.gene067248:CDS
GCAGTTGTAACAGATGGTGA+TGG 0.582363 8.1:+59162332 MS.gene067248:CDS
AGCATATAACATTGGATCGG+TGG 0.585153 8.1:-59162036 None:intergenic
CAAATATAATGGTCTGTGAA+TGG 0.585642 8.1:-59162220 None:intergenic
CCCGAACAAATTGTGAAGCA+AGG 0.585769 8.1:+59161682 MS.gene067248:CDS
TTTCTGAACAACGATTCCAA+TGG 0.587464 8.1:+59163148 MS.gene067248:CDS
AAGACATATGTCAGAGCGAA+AGG 0.588375 8.1:+59162677 MS.gene067248:CDS
GGATATATGGTTGCTCAGAA+GGG 0.589834 8.1:+59161891 MS.gene067248:CDS
AAGAAAACACCATTTCTGGA+GGG 0.593121 8.1:+59162446 MS.gene067248:CDS
AACAATCGAGTCATATAAGT+GGG 0.594755 8.1:+59162265 MS.gene067248:CDS
GATAGCTTCACATTGAGATG+TGG 0.595390 8.1:-59162642 None:intergenic
TGTAGTGTCTAAATTTGAAG+AGG 0.597171 8.1:+59161740 MS.gene067248:CDS
TGAGAAAGGCATTGAAAGAG+AGG 0.597237 8.1:+59162961 MS.gene067248:CDS
ACAATGCATTAGTAGGTGAA+AGG 0.599354 8.1:+59161415 MS.gene067248:CDS
CGCCATGAAGTCCATAATTG+GGG 0.600485 8.1:-59163936 None:intergenic
TGATGAATCAGTTCAACAAG+AGG 0.603374 8.1:+59161383 MS.gene067248:CDS
AAAGAAAACACCATTTCTGG+AGG 0.604034 8.1:+59162445 MS.gene067248:CDS
CTCAAGAGCAAAGAATGTGT+AGG 0.605188 8.1:+59162905 MS.gene067248:CDS
GATGTTAGGAGCAATAGTAG+TGG 0.605946 8.1:+59161561 MS.gene067248:CDS
CAAGTGTGTAAATTTAGGTG+GGG 0.607267 8.1:-59161781 None:intergenic
ATGCGTGATGTCATTAAAGA+CGG 0.612992 8.1:+59161972 MS.gene067248:CDS
TATATGGTTGCTCAGAAGGG+TGG 0.613989 8.1:+59161894 MS.gene067248:CDS
CGCAGGTGAGTCGTCAACAC+AGG 0.614146 8.1:-59162794 None:intergenic
TTCAGCATATAACATTGGAT+CGG 0.614583 8.1:-59162039 None:intergenic
GAGCACCTTTACTCTTCACA+GGG 0.616222 8.1:-59163375 None:intergenic
GGCGCTGCTGGGTCGCACGT+GGG 0.618011 8.1:-59160353 None:intergenic
AACCTGAGGCTTGGAGAGCG+AGG 0.625854 8.1:-59160395 None:intergenic
AAGTTTAAGAATTTCATCAG+AGG 0.626746 8.1:-59161460 None:intergenic
AAATTAACAGATGATCAGGT+AGG 0.630827 8.1:+59163343 MS.gene067248:CDS
TCGTATTCAGCATATAACAT+TGG 0.632685 8.1:-59162044 None:intergenic
TGTAGACTATTTGAGACACG+TGG 0.633840 8.1:+59163022 MS.gene067248:CDS
GGCTGGAACACATGAACAGT+AGG 0.634224 8.1:-59163880 None:intergenic
AGATGGTGATGGATCAATGA+GGG 0.640655 8.1:+59162343 MS.gene067248:CDS
GAAGTACAAGTCATACTGCA+AGG 0.642891 8.1:+59163449 MS.gene067248:CDS
CCCAATTATGGACTTCATGG+CGG 0.651938 8.1:+59163937 MS.gene067248:CDS
GAACTTATCACGTAAATATG+CGG 0.655600 8.1:-59162603 None:intergenic
AGAATGTGTAGGGGATACCA+TGG 0.663034 8.1:+59162916 MS.gene067248:CDS
ATACGCGAACAAGTCACTGT+GGG 0.666824 8.1:+59162577 MS.gene067248:CDS
GCACACGAGAGAGAAAGAAG+AGG 0.667424 8.1:-59160326 None:intergenic
GATGAATCAGTTCAACAAGA+GGG 0.669122 8.1:+59161384 MS.gene067248:CDS
GTGATACTGAATACTCTACT+CGG 0.673362 8.1:+59163314 MS.gene067248:CDS
TAGTGTCTAAATTTGAAGAG+GGG 0.673727 8.1:+59161742 MS.gene067248:CDS
CATCACTCTCAATCGAACGC+AGG 0.676080 8.1:-59162811 None:intergenic
CTGAGGCTTGGAGAGCGAGG+TGG 0.685584 8.1:-59160392 None:intergenic
CACCCAGAAAATATACACAA+CGG 0.691486 8.1:-59162191 None:intergenic
CAAGAGCAAAGAATGTGTAG+GGG 0.691842 8.1:+59162907 MS.gene067248:CDS
ATATAATGGTCTGTGAATGG+TGG 0.703942 8.1:-59162217 None:intergenic
TCTGAACAACGATTCCAATG+GGG 0.705472 8.1:+59163150 MS.gene067248:CDS
ATAAGCGATGAGATGCATCA+GGG 0.712304 8.1:-59162385 None:intergenic
CAGATGGTGATGGATCAATG+AGG 0.720761 8.1:+59162342 MS.gene067248:CDS
GTAGACTATTTGAGACACGT+GGG 0.732024 8.1:+59163023 MS.gene067248:CDS
CATACGCGAACAAGTCACTG+TGG 0.733360 8.1:+59162576 MS.gene067248:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTAAATTTTTGAAATTTT+AGG + chr8.1:59161217-59161236 MS.gene067248:intron 10.0%
!!! TATGAATTTTATTATCGTTA+TGG + chr8.1:59161507-59161526 MS.gene067248:CDS 15.0%
!! AAAAAAGAATGGTGTATTTA+GGG + chr8.1:59163252-59163271 MS.gene067248:CDS 20.0%
!! AAAAATAAAAGGAAACCTAA+TGG + chr8.1:59163760-59163779 MS.gene067248:CDS 20.0%
!! AACCCTAATTAAAAAGAAAA+AGG - chr8.1:59160463-59160482 None:intergenic 20.0%
!! ACTAGGTTATAACAAAAAAA+AGG + chr8.1:59160851-59160870 MS.gene067248:intron 20.0%
!! AGTTAAAGATGAAATTATTG+AGG + chr8.1:59162868-59162887 MS.gene067248:CDS 20.0%
!! GAAATTCTTAAACTTGAATT+TGG + chr8.1:59161468-59161487 MS.gene067248:CDS 20.0%
!! GTTATATACTTGTTTAGTAT+TGG + chr8.1:59160885-59160904 MS.gene067248:intron 20.0%
!! TGAATGTAATAGTGTTTAAT+TGG + chr8.1:59160506-59160525 MS.gene067248:intron 20.0%
!!! AATTGGAGATTATTTTTTTG+GGG + chr8.1:59160629-59160648 MS.gene067248:intron 20.0%
!!! ATTTATGCATAATTTTGAAC+AGG + chr8.1:59162712-59162731 MS.gene067248:CDS 20.0%
!!! CAATTGGAGATTATTTTTTT+GGG + chr8.1:59160628-59160647 MS.gene067248:intron 20.0%
!!! CTCCTTTTTCTTTTTAATTA+GGG + chr8.1:59160458-59160477 MS.gene067248:intron 20.0%
!!! TAATTAGGGTTTTTTGATTT+TGG + chr8.1:59160472-59160491 MS.gene067248:intron 20.0%
!!! TCTCCTTTTTCTTTTTAATT+AGG + chr8.1:59160457-59160476 MS.gene067248:intron 20.0%
!!! TTTCCATACATTCTAAAAAA+CGG - chr8.1:59162298-59162317 None:intergenic 20.0%
!!! TTTTGCTTGCAATAATATAT+TGG + chr8.1:59161063-59161082 MS.gene067248:intron 20.0%
! AAGTTTAAGAATTTCATCAG+AGG - chr8.1:59161463-59161482 None:intergenic 25.0%
! ACAAAAAAAACATGACAACA+TGG - chr8.1:59163052-59163071 None:intergenic 25.0%
! ATGAACTTGAAGAAAATGAT+TGG + chr8.1:59162543-59162562 MS.gene067248:CDS 25.0%
! ATTATGAAATTGGCATTATG+AGG + chr8.1:59160536-59160555 MS.gene067248:intron 25.0%
! CAAAAAAGAATGGTGTATTT+AGG + chr8.1:59163251-59163270 MS.gene067248:CDS 25.0%
! CAAGCAAAAGAAATATAAAG+AGG + chr8.1:59163798-59163817 MS.gene067248:CDS 25.0%
! GTCATTCACAGAAAAATAAA+AGG + chr8.1:59163749-59163768 MS.gene067248:intron 25.0%
! TATTTACGTGATAAGTTCTT+TGG + chr8.1:59162608-59162627 MS.gene067248:CDS 25.0%
! TGAACTTGAAGAAAATGATT+GGG + chr8.1:59162544-59162563 MS.gene067248:CDS 25.0%
! TGGTTGATTGATTATGAAAT+TGG + chr8.1:59160526-59160545 MS.gene067248:intron 25.0%
! TTCTTTAAGCCAAAAAGAAA+AGG + chr8.1:59163659-59163678 MS.gene067248:CDS 25.0%
!! AAGAATGAAAAGTCTGTTTT+GGG + chr8.1:59162082-59162101 MS.gene067248:CDS 25.0%
!! AATCACAAAATCGCAAATTT+TGG - chr8.1:59160605-59160624 None:intergenic 25.0%
!! AATGTACTCAAATTTTACAC+CGG + chr8.1:59162481-59162500 MS.gene067248:CDS 25.0%
!! ACAAACATACCTTTTCTTTT+TGG - chr8.1:59163671-59163690 None:intergenic 25.0%
!! ATAAGCAAGTTAATTTTTGC+AGG + chr8.1:59163578-59163597 MS.gene067248:intron 25.0%
!! ATATACTTGTTTAGTATTGG+AGG + chr8.1:59160888-59160907 MS.gene067248:intron 25.0%
!! TGCCATTTATATGTACATTT+GGG + chr8.1:59160968-59160987 MS.gene067248:intron 25.0%
!!! AAATACACCATTCTTTTTTG+AGG - chr8.1:59163251-59163270 None:intergenic 25.0%
!!! AGGTCTGATTATTTTTGTTT+TGG + chr8.1:59162119-59162138 MS.gene067248:CDS 25.0%
!!! GCAATTGGAGATTATTTTTT+TGG + chr8.1:59160627-59160646 MS.gene067248:intron 25.0%
!!! TCATGTTTTTTTTGTGATGA+AGG + chr8.1:59163057-59163076 MS.gene067248:CDS 25.0%
AAACAATCGAGTCATATAAG+TGG + chr8.1:59162264-59162283 MS.gene067248:CDS 30.0%
AAAGAAGCCTCAAAAAAGAA+TGG + chr8.1:59163241-59163260 MS.gene067248:CDS 30.0%
AAAGATGAAATTATTGAGGC+TGG + chr8.1:59162872-59162891 MS.gene067248:CDS 30.0%
AAATTAACAGATGATCAGGT+AGG + chr8.1:59163343-59163362 MS.gene067248:CDS 30.0%
AACAATCGAGTCATATAAGT+GGG + chr8.1:59162265-59162284 MS.gene067248:CDS 30.0%
AAGACGTAGATTTCATAGTT+TGG - chr8.1:59161133-59161152 None:intergenic 30.0%
AATCAGAACCTGTCATATAA+TGG + chr8.1:59161869-59161888 MS.gene067248:CDS 30.0%
AGAATTGGTATCTAACTCTA+TGG + chr8.1:59163632-59163651 MS.gene067248:CDS 30.0%
AGCACAATTTACAGAGATTT+CGG + chr8.1:59163822-59163841 MS.gene067248:CDS 30.0%
ATCAGAACCTGTCATATAAT+GGG + chr8.1:59161870-59161889 MS.gene067248:CDS 30.0%
ATGATTGAAGTTGCTAGATT+TGG + chr8.1:59163187-59163206 MS.gene067248:CDS 30.0%
ATTTGCATTGGATTAATAGC+AGG - chr8.1:59163904-59163923 None:intergenic 30.0%
CAAATATAATGGTCTGTGAA+TGG - chr8.1:59162223-59162242 None:intergenic 30.0%
CACAATGAACCAAAACAATA+TGG - chr8.1:59160742-59160761 None:intergenic 30.0%
CACCAAATTAGTACCAATTT+CGG - chr8.1:59160656-59160675 None:intergenic 30.0%
CACCCAAATGTACATATAAA+TGG - chr8.1:59160973-59160992 None:intergenic 30.0%
CATATATCCCATTATATGAC+AGG - chr8.1:59161880-59161899 None:intergenic 30.0%
CATTCACAGACCATTATATT+TGG + chr8.1:59162221-59162240 MS.gene067248:CDS 30.0%
CATTTATATGTACATTTGGG+TGG + chr8.1:59160971-59160990 MS.gene067248:intron 30.0%
GAACTTATCACGTAAATATG+CGG - chr8.1:59162606-59162625 None:intergenic 30.0%
GTAGTGTCTAAATTTGAAGA+GGG + chr8.1:59161741-59161760 MS.gene067248:CDS 30.0%
TAGTGTCTAAATTTGAAGAG+GGG + chr8.1:59161742-59161761 MS.gene067248:CDS 30.0%
TAGTTATTAAAGCAGACACT+AGG + chr8.1:59160834-59160853 MS.gene067248:intron 30.0%
TCAATCAACAATGCATTAGT+AGG + chr8.1:59161408-59161427 MS.gene067248:CDS 30.0%
TCGTATTCAGCATATAACAT+TGG - chr8.1:59162047-59162066 None:intergenic 30.0%
TGAGAAATTAACAGATGATC+AGG + chr8.1:59163339-59163358 MS.gene067248:CDS 30.0%
TGATCACATACCAAAATGTT+TGG + chr8.1:59163090-59163109 MS.gene067248:CDS 30.0%
TGTAAAGAAAACACCATTTC+TGG + chr8.1:59162442-59162461 MS.gene067248:CDS 30.0%
TGTAGTGTCTAAATTTGAAG+AGG + chr8.1:59161740-59161759 MS.gene067248:CDS 30.0%
TGTTGATATGAATCTGTTGA+GGG + chr8.1:59160683-59160702 MS.gene067248:intron 30.0%
TTCAGCATATAACATTGGAT+CGG - chr8.1:59162042-59162061 None:intergenic 30.0%
TTGGTTCATTGTGTAATTCA+AGG + chr8.1:59160749-59160768 MS.gene067248:intron 30.0%
TTGTTGATATGAATCTGTTG+AGG + chr8.1:59160682-59160701 MS.gene067248:intron 30.0%
! ATCCGTTGTGTATATTTTCT+GGG + chr8.1:59162189-59162208 MS.gene067248:CDS 30.0%
! CGATTTTGTGATTATGCAAT+TGG + chr8.1:59160612-59160631 MS.gene067248:intron 30.0%
! CTGCCATTTATATGTACATT+TGG + chr8.1:59160967-59160986 MS.gene067248:intron 30.0%
! CTGTCATATAATGGGATATA+TGG + chr8.1:59161878-59161897 MS.gene067248:CDS 30.0%
! GAAGAATGAAAAGTCTGTTT+TGG + chr8.1:59162081-59162100 MS.gene067248:CDS 30.0%
! TATCCGTTGTGTATATTTTC+TGG + chr8.1:59162188-59162207 MS.gene067248:CDS 30.0%
!! CAAAACAATATGGCTTGTTT+GGG - chr8.1:59160732-59160751 None:intergenic 30.0%
!!! GAACCGTTTTTTAGAATGTA+TGG + chr8.1:59162292-59162311 MS.gene067248:CDS 30.0%
!!! TTGAAATTTTAGGGTTGTCA+TGG + chr8.1:59161227-59161246 MS.gene067248:intron 30.0%
AACACAATTGAGCTACTTCA+AGG + chr8.1:59160803-59160822 MS.gene067248:intron 35.0%
AAGAATTTCATCAGAGGCAA+TGG - chr8.1:59161457-59161476 None:intergenic 35.0%
AATCATCAACTTCCTGTTCT+GGG + chr8.1:59163962-59163981 MS.gene067248:CDS 35.0%
AATGAGGGAAGCTATTAAAC+AGG + chr8.1:59162358-59162377 MS.gene067248:CDS 35.0%
ACAATGCATTAGTAGGTGAA+AGG + chr8.1:59161415-59161434 MS.gene067248:CDS 35.0%
ACATGAACATGATGCAGAAT+TGG + chr8.1:59163617-59163636 MS.gene067248:CDS 35.0%
AGTCCATAATTGGGGTAAAT+TGG - chr8.1:59163931-59163950 None:intergenic 35.0%
AGTGTTGTGAAACCATTGTT+AGG + chr8.1:59160567-59160586 MS.gene067248:intron 35.0%
ATATAATGGTCTGTGAATGG+TGG - chr8.1:59162220-59162239 None:intergenic 35.0%
ATCTGTGTATTCTACTTCAC+TGG - chr8.1:59161283-59161302 None:intergenic 35.0%
ATGCGTGATGTCATTAAAGA+CGG + chr8.1:59161972-59161991 MS.gene067248:CDS 35.0%
ATTTAGGTGGGGTTAATTCA+TGG - chr8.1:59161773-59161792 None:intergenic 35.0%
CAAGTGTGTAAATTTAGGTG+GGG - chr8.1:59161784-59161803 None:intergenic 35.0%
CACCCAGAAAATATACACAA+CGG - chr8.1:59162194-59162213 None:intergenic 35.0%
CTTGTGCTAACATTACCATT+AGG - chr8.1:59163778-59163797 None:intergenic 35.0%
GAATACGATGTCAACATTGA+TGG + chr8.1:59162059-59162078 MS.gene067248:CDS 35.0%
GATGAATCAGTTCAACAAGA+GGG + chr8.1:59161384-59161403 MS.gene067248:CDS 35.0%
GCAAGTGTGTAAATTTAGGT+GGG - chr8.1:59161785-59161804 None:intergenic 35.0%
GTACGTTACAAAGCCAAAAA+GGG + chr8.1:59161711-59161730 MS.gene067248:CDS 35.0%
GTAGATTTCATAGTTTGGCT+AGG - chr8.1:59161128-59161147 None:intergenic 35.0%
GTAGCATTCTTCTTCTTCTT+TGG - chr8.1:59163400-59163419 None:intergenic 35.0%
GTATTCTACTTCACTGGAAT+CGG - chr8.1:59161277-59161296 None:intergenic 35.0%
GTCCGAAATTGGTACTAATT+TGG + chr8.1:59160651-59160670 MS.gene067248:intron 35.0%
GTGATACTGAATACTCTACT+CGG + chr8.1:59163314-59163333 MS.gene067248:CDS 35.0%
GTGTTGTGAAACCATTGTTA+GGG + chr8.1:59160568-59160587 MS.gene067248:intron 35.0%
TAAATTGGCTGCATTTGCAT+TGG - chr8.1:59163916-59163935 None:intergenic 35.0%
TACGTTACAAAGCCAAAAAG+GGG + chr8.1:59161712-59161731 MS.gene067248:CDS 35.0%
TATAAATGGCAGCACACAAA+TGG - chr8.1:59160959-59160978 None:intergenic 35.0%
TATCGTTATGGAAGATGCAA+AGG + chr8.1:59161519-59161538 MS.gene067248:CDS 35.0%
TCAAGAGCAAAGAATGTGTA+GGG + chr8.1:59162906-59162925 MS.gene067248:CDS 35.0%
TCTTCCTAAGACAAGAAAGA+AGG - chr8.1:59161040-59161059 None:intergenic 35.0%
TCTTTCTTGTCTTAGGAAGA+TGG + chr8.1:59161040-59161059 MS.gene067248:intron 35.0%
TGATGAATCAGTTCAACAAG+AGG + chr8.1:59161383-59161402 MS.gene067248:CDS 35.0%
TGCAAGTGTGTAAATTTAGG+TGG - chr8.1:59161786-59161805 None:intergenic 35.0%
TGGTGTATTTAGGGACATAA+TGG + chr8.1:59163261-59163280 MS.gene067248:CDS 35.0%
TGTACGTTACAAAGCCAAAA+AGG + chr8.1:59161710-59161729 MS.gene067248:CDS 35.0%
TTCTGAACAACGATTCCAAT+GGG + chr8.1:59163149-59163168 MS.gene067248:CDS 35.0%
TTGCGAAAATATCGTGAGAA+AGG + chr8.1:59162947-59162966 MS.gene067248:CDS 35.0%
TTTCATAGTTTGGCTAGGTA+TGG - chr8.1:59161123-59161142 None:intergenic 35.0%
TTTCTGAACAACGATTCCAA+TGG + chr8.1:59163148-59163167 MS.gene067248:CDS 35.0%
! AAAGAAAACACCATTTCTGG+AGG + chr8.1:59162445-59162464 MS.gene067248:CDS 35.0%
! AAAGGTTTTGCCATTAGGAA+AGG + chr8.1:59161537-59161556 MS.gene067248:CDS 35.0%
! AAGAAAACACCATTTCTGGA+GGG + chr8.1:59162446-59162465 MS.gene067248:CDS 35.0%
! CAGAGATTTTACGATACAGT+GGG - chr8.1:59161809-59161828 None:intergenic 35.0%
! CCAAACAAGCCATATTGTTT+TGG + chr8.1:59160730-59160749 MS.gene067248:intron 35.0%
! TCAGAGATTTTACGATACAG+TGG - chr8.1:59161810-59161829 None:intergenic 35.0%
! TTGGCACAAGTCATGAATTT+TGG + chr8.1:59163988-59164007 MS.gene067248:CDS 35.0%
! TTTTCGCAATGTGTAAACCA+TGG - chr8.1:59162936-59162955 None:intergenic 35.0%
! TTTTTGCAGGATACACAGAA+CGG + chr8.1:59163591-59163610 MS.gene067248:intron 35.0%
!! CATCTGTTACAACTGCTTTT+GGG - chr8.1:59162328-59162347 None:intergenic 35.0%
!! CCAAAACAATATGGCTTGTT+TGG - chr8.1:59160733-59160752 None:intergenic 35.0%
!! TGATTGAAGAGCATCAAGTT+GGG - chr8.1:59161844-59161863 None:intergenic 35.0%
!!! ATTTTTTTGGGGTCCGAAAT+TGG + chr8.1:59160640-59160659 MS.gene067248:intron 35.0%
!!! TTTTTGTTTTGGCGACGTTT+TGG + chr8.1:59162130-59162149 MS.gene067248:CDS 35.0%
AAGACATATGTCAGAGCGAA+AGG + chr8.1:59162677-59162696 MS.gene067248:CDS 40.0%
AATGTTTGGTTATGACCCGT+TGG + chr8.1:59163104-59163123 MS.gene067248:CDS 40.0%
AGCACCTTCTTTCTTGTCTT+AGG + chr8.1:59161033-59161052 MS.gene067248:intron 40.0%
AGCATATAACATTGGATCGG+TGG - chr8.1:59162039-59162058 None:intergenic 40.0%
AGTGTTAGAAAACGAACCTG+AGG - chr8.1:59160412-59160431 None:intergenic 40.0%
ATAAGCGATGAGATGCATCA+GGG - chr8.1:59162388-59162407 None:intergenic 40.0%
ATCAGAATCCATTTCCCCAT+TGG - chr8.1:59163167-59163186 None:intergenic 40.0%
ATTGGAGGCAACAATTACGT+TGG + chr8.1:59160903-59160922 MS.gene067248:intron 40.0%
CAAAACTCCTCAAATTGCTC+CGG - chr8.1:59162503-59162522 None:intergenic 40.0%
CAACGCAACGCCAAATATAA+TGG - chr8.1:59162234-59162253 None:intergenic 40.0%
CAAGAGCAAAGAATGTGTAG+GGG + chr8.1:59162907-59162926 MS.gene067248:CDS 40.0%
CAGCCAATTTACCCCAATTA+TGG + chr8.1:59163925-59163944 MS.gene067248:CDS 40.0%
CCAAAAGCAGTTGTAACAGA+TGG + chr8.1:59162326-59162345 MS.gene067248:CDS 40.0%
CCACATCAAAACCCTAACAA+TGG - chr8.1:59160582-59160601 None:intergenic 40.0%
CCATTAGGAAAGGTGATGTT+AGG + chr8.1:59161547-59161566 MS.gene067248:CDS 40.0%
CCTAACATCACCTTTCCTAA+TGG - chr8.1:59161550-59161569 None:intergenic 40.0%
CTCAAGAGCAAAGAATGTGT+AGG + chr8.1:59162905-59162924 MS.gene067248:CDS 40.0%
CTTGATGCTCTTCAATCACA+TGG + chr8.1:59161846-59161865 MS.gene067248:CDS 40.0%
GAAGTACAAGTCATACTGCA+AGG + chr8.1:59163449-59163468 MS.gene067248:CDS 40.0%
GAATCATCAACTTCCTGTTC+TGG + chr8.1:59163961-59163980 MS.gene067248:CDS 40.0%
GATAGCTTCACATTGAGATG+TGG - chr8.1:59162645-59162664 None:intergenic 40.0%
GATATGAATCTGTTGAGGGT+TGG + chr8.1:59160687-59160706 MS.gene067248:intron 40.0%
GATCTCTGCTGTGTAAATCT+TGG - chr8.1:59162840-59162859 None:intergenic 40.0%
GATGCTGAAGATGATGTTGA+TGG + chr8.1:59161339-59161358 MS.gene067248:CDS 40.0%
GATGTTAGGAGCAATAGTAG+TGG + chr8.1:59161561-59161580 MS.gene067248:CDS 40.0%
GGAACACCACAAAGATTGTT+TGG + chr8.1:59161248-59161267 MS.gene067248:CDS 40.0%
GGATATATGGTTGCTCAGAA+GGG + chr8.1:59161891-59161910 MS.gene067248:CDS 40.0%
GGGTGCAAGTGTGTAAATTT+AGG - chr8.1:59161789-59161808 None:intergenic 40.0%
TACCCCAATTATGGACTTCA+TGG + chr8.1:59163934-59163953 MS.gene067248:CDS 40.0%
TATATTTGGCGTTGCGTTGT+TGG + chr8.1:59162235-59162254 MS.gene067248:CDS 40.0%
TCTGAACAACGATTCCAATG+GGG + chr8.1:59163150-59163169 MS.gene067248:CDS 40.0%
TGAAATCTACGTCTTGTGCT+AGG + chr8.1:59161138-59161157 MS.gene067248:intron 40.0%
TGCATTAGTAGGTGAAAGGT+TGG + chr8.1:59161419-59161438 MS.gene067248:CDS 40.0%
TTTCCAAAACCCTCCAGAAA+TGG - chr8.1:59162458-59162477 None:intergenic 40.0%
! CACTACATATCTCCCCTTTT+TGG - chr8.1:59161727-59161746 None:intergenic 40.0%
! CCATCTGTTACAACTGCTTT+TGG - chr8.1:59162329-59162348 None:intergenic 40.0%
! CCTTGCTTCACAATTTGTTC+GGG - chr8.1:59161685-59161704 None:intergenic 40.0%
! CGGGTCATAACCAAACATTT+TGG - chr8.1:59163103-59163122 None:intergenic 40.0%
! CTTTTGCGTTCTTTGTCCAA+CGG - chr8.1:59163123-59163142 None:intergenic 40.0%
! GATGCAAAGGTTTTGCCATT+AGG + chr8.1:59161532-59161551 MS.gene067248:CDS 40.0%
! GCACAAGTCATGAATTTTGG+TGG + chr8.1:59163991-59164010 MS.gene067248:CDS 40.0%
! GCCTTGCTTCACAATTTGTT+CGG - chr8.1:59161686-59161705 None:intergenic 40.0%
! GTAGACTATTTGAGACACGT+GGG + chr8.1:59163023-59163042 MS.gene067248:CDS 40.0%
! TGTAGACTATTTGAGACACG+TGG + chr8.1:59163022-59163041 MS.gene067248:CDS 40.0%
! TTTTACACCGGAGCAATTTG+AGG + chr8.1:59162493-59162512 MS.gene067248:CDS 40.0%
! TTTTGCGTTCTTTGTCCAAC+GGG - chr8.1:59163122-59163141 None:intergenic 40.0%
!! AAAAGTCTGTTTTGGGCTGA+TGG + chr8.1:59162089-59162108 MS.gene067248:CDS 40.0%
!! AAAGTCTGTTTTGGGCTGAT+GGG + chr8.1:59162090-59162109 MS.gene067248:CDS 40.0%
!! AGATGGTGATGGATCAATGA+GGG + chr8.1:59162343-59162362 MS.gene067248:CDS 40.0%
!! CCATTGTTAGGGTTTTGATG+TGG + chr8.1:59160579-59160598 MS.gene067248:intron 40.0%
!! GAGACAAGAAGCACTTATCT+AGG + chr8.1:59161628-59161647 MS.gene067248:CDS 40.0%
!! GTCATGAATTTTGGTGGTCA+TGG + chr8.1:59163997-59164016 MS.gene067248:CDS 40.0%
!! GTGATTGAAGAGCATCAAGT+TGG - chr8.1:59161845-59161864 None:intergenic 40.0%
!! TCATACTGCAAGGCATTGTT+CGG + chr8.1:59163459-59163478 MS.gene067248:CDS 40.0%
!! TGAGAAAGGCATTGAAAGAG+AGG + chr8.1:59162961-59162980 MS.gene067248:CDS 40.0%
AACCCCAGCGTATATGTTAC+AGG - chr8.1:59160998-59161017 None:intergenic 45.0%
ACCCCAGCGTATATGTTACA+GGG - chr8.1:59160997-59161016 None:intergenic 45.0%
ACCGCCATGAAGTCCATAAT+TGG - chr8.1:59163941-59163960 None:intergenic 45.0%
AGAATGTGTAGGGGATACCA+TGG + chr8.1:59162916-59162935 MS.gene067248:CDS 45.0%
ATACGCGAACAAGTCACTGT+GGG + chr8.1:59162577-59162596 MS.gene067248:CDS 45.0%
CAACGATTCCAATGGGGAAA+TGG + chr8.1:59163156-59163175 MS.gene067248:CDS 45.0%
CATAAGCGATGAGATGCATC+AGG - chr8.1:59162389-59162408 None:intergenic 45.0%
CCAATCCCTGTGAAGAGTAA+AGG + chr8.1:59163370-59163389 MS.gene067248:CDS 45.0%
CCCAATTATGGACTTCATGG+CGG + chr8.1:59163937-59163956 MS.gene067248:CDS 45.0%
CCCGAACAAATTGTGAAGCA+AGG + chr8.1:59161682-59161701 MS.gene067248:CDS 45.0%
CCGCCATGAAGTCCATAATT+GGG - chr8.1:59163940-59163959 None:intergenic 45.0%
CCTTTACTCTTCACAGGGAT+TGG - chr8.1:59163373-59163392 None:intergenic 45.0%
CGCCATGAAGTCCATAATTG+GGG - chr8.1:59163939-59163958 None:intergenic 45.0%
GAGAGAGAAAGAAGAGGCAA+CGG - chr8.1:59160323-59160342 None:intergenic 45.0%
GAGCACCTTTACTCTTCACA+GGG - chr8.1:59163378-59163397 None:intergenic 45.0%
GAGTTTGTGTGCAACAAGCA+AGG + chr8.1:59161600-59161619 MS.gene067248:CDS 45.0%
GCAGTTGTAACAGATGGTGA+TGG + chr8.1:59162332-59162351 MS.gene067248:CDS 45.0%
GCATTGGATTAATAGCAGGC+TGG - chr8.1:59163900-59163919 None:intergenic 45.0%
GGGATATATGGTTGCTCAGA+AGG + chr8.1:59161890-59161909 MS.gene067248:CDS 45.0%
GGTTGAACTGCAGTTGTTAC+CGG - chr8.1:59163862-59163881 None:intergenic 45.0%
TAGAAAACGAACCTGAGGCT+TGG - chr8.1:59160407-59160426 None:intergenic 45.0%
TATATGGTTGCTCAGAAGGG+TGG + chr8.1:59161894-59161913 MS.gene067248:CDS 45.0%
! CGGAGCAATTTGAGGAGTTT+TGG + chr8.1:59162501-59162520 MS.gene067248:CDS 45.0%
!! ACACCATTTCTGGAGGGTTT+TGG + chr8.1:59162452-59162471 MS.gene067248:CDS 45.0%
!! CAGATGGTGATGGATCAATG+AGG + chr8.1:59162342-59162361 MS.gene067248:CDS 45.0%
!!! TTTAAATTTTTGAAATTTTA+GGG + chr8.1:59161218-59161237 MS.gene067248:intron 5.0%
AAGGGTGGATACGCTGATGT+AGG + chr8.1:59161909-59161928 MS.gene067248:CDS 50.0%
AGAAAGAAGAGGCAACGGAG+TGG - chr8.1:59160318-59160337 None:intergenic 50.0%
ATTTGGCGTTGCGTTGTTGG+AGG + chr8.1:59162238-59162257 MS.gene067248:CDS 50.0%
CATACGCGAACAAGTCACTG+TGG + chr8.1:59162576-59162595 MS.gene067248:CDS 50.0%
CATCACTCTCAATCGAACGC+AGG - chr8.1:59162814-59162833 None:intergenic 50.0%
CATCTCATCGCTTATGTGCC+TGG + chr8.1:59162393-59162412 MS.gene067248:CDS 50.0%
CGGCGTCCAAACAATCTTTG+TGG - chr8.1:59161257-59161276 None:intergenic 50.0%
GAAAGAAGAGGCAACGGAGT+GGG - chr8.1:59160317-59160336 None:intergenic 50.0%
GCACACGAGAGAGAAAGAAG+AGG - chr8.1:59160329-59160348 None:intergenic 50.0%
GCCCTGTAACATATACGCTG+GGG + chr8.1:59160993-59161012 MS.gene067248:intron 50.0%
GGCCCTGTAACATATACGCT+GGG + chr8.1:59160992-59161011 MS.gene067248:intron 50.0%
GGCTGGAACACATGAACAGT+AGG - chr8.1:59163883-59163902 None:intergenic 50.0%
TGGCCCTGTAACATATACGC+TGG + chr8.1:59160991-59161010 MS.gene067248:intron 50.0%
TTTGGGCTGATGGGAGTAGT+AGG + chr8.1:59162099-59162118 MS.gene067248:CDS 50.0%
! AACTTCCTGTTCTGGGCTCT+TGG + chr8.1:59163969-59163988 MS.gene067248:CDS 50.0%
! GGAGCACCTTTACTCTTCAC+AGG - chr8.1:59163379-59163398 None:intergenic 50.0%
!! TTCGGAGACAGCTGATTTGC+CGG + chr8.1:59163840-59163859 MS.gene067248:CDS 50.0%
CGCGTTCTTATGCAGATGCC+AGG - chr8.1:59162414-59162433 None:intergenic 55.0%
TTGTGCCAAGAGCCCAGAAC+AGG - chr8.1:59163977-59163996 None:intergenic 55.0%
AACCTGAGGCTTGGAGAGCG+AGG - chr8.1:59160398-59160417 None:intergenic 60.0%
CGCAGGTGAGTCGTCAACAC+AGG - chr8.1:59162797-59162816 None:intergenic 60.0%
GGAGTGAAGATGGCGCTGCT+GGG - chr8.1:59160367-59160386 None:intergenic 60.0%
!! GGTGGTGGACGGAGTGAAGA+TGG - chr8.1:59160377-59160396 None:intergenic 60.0%
!! TTGGAGAGCGAGGTGGTGGA+CGG - chr8.1:59160388-59160407 None:intergenic 60.0%
AGGCTTGGAGAGCGAGGTGG+TGG - chr8.1:59160392-59160411 None:intergenic 65.0%
CACCTCGCTCTCCAAGCCTC+AGG + chr8.1:59160393-59160412 MS.gene067248:CDS 65.0%
CGGAGTGAAGATGGCGCTGC+TGG - chr8.1:59160368-59160387 None:intergenic 65.0%
CTGAGGCTTGGAGAGCGAGG+TGG - chr8.1:59160395-59160414 None:intergenic 65.0%
GGCGCTGCTGGGTCGCACGT+GGG - chr8.1:59160356-59160375 None:intergenic 75.0%
TGGCGCTGCTGGGTCGCACG+TGG - chr8.1:59160357-59160376 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 59160308 59164050 59160308 ID=MS.gene067248
chr8.1 mRNA 59160308 59164050 59160308 ID=MS.gene067248.t1;Parent=MS.gene067248
chr8.1 exon 59160308 59160414 59160308 ID=MS.gene067248.t1.exon1;Parent=MS.gene067248.t1
chr8.1 CDS 59160308 59160414 59160308 ID=cds.MS.gene067248.t1;Parent=MS.gene067248.t1
chr8.1 exon 59161239 59163480 59161239 ID=MS.gene067248.t1.exon2;Parent=MS.gene067248.t1
chr8.1 CDS 59161239 59163480 59161239 ID=cds.MS.gene067248.t1;Parent=MS.gene067248.t1
chr8.1 exon 59163600 59163680 59163600 ID=MS.gene067248.t1.exon3;Parent=MS.gene067248.t1
chr8.1 CDS 59163600 59163680 59163600 ID=cds.MS.gene067248.t1;Parent=MS.gene067248.t1
chr8.1 exon 59163760 59164050 59163760 ID=MS.gene067248.t1.exon4;Parent=MS.gene067248.t1
chr8.1 CDS 59163760 59164050 59163760 ID=cds.MS.gene067248.t1;Parent=MS.gene067248.t1
Gene Sequence

>MS.gene067248

ATGCGACCCACTCCGTTGCCTCTTCTTTCTCTCTCGTGTGCGCAACCCACGTGCGACCCAGCAGCGCCATCTTCACTCCGTCCACCACCTCGCTCTCCAAGCCTCAGGTTCGTTTTCTAACACTCGACAGAACAATCTGAATGAATGAATCTCCTTTTTCTTTTTAATTAGGGTTTTTTGATTTTGGATTCTTTTATATGAATGTAATAGTGTTTAATTGGTTGATTGATTATGAAATTGGCATTATGAGGAATATATTAGTGTTGTGAAACCATTGTTAGGGTTTTGATGTGGCCAAAATTTGCGATTTTGTGATTATGCAATTGGAGATTATTTTTTTGGGGTCCGAAATTGGTACTAATTTGGTGTTATTGTTGTTGATATGAATCTGTTGAGGGTTGGTGTTTTTGCTCTTGATATGCCCAAACAAGCCATATTGTTTTGGTTCATTGTGTAATTCAAGGACTGTTATTTCACTTTTCATATCAATCTTCTAACACAATTGAGCTACTTCAAGGATGTAGTGTAGTTATTAAAGCAGACACTAGGTTATAACAAAAAAAAGGTTTTCAACAATGTTATATACTTGTTTAGTATTGGAGGCAACAATTACGTTGGCATTCACTACATCTTATATTGTCTTTGTTTCCATTTGTGTGCTGCCATTTATATGTACATTTGGGTGGCCCTGTAACATATACGCTGGGGTTTTTGTTTGTTTTCTTAGCACCTTCTTTCTTGTCTTAGGAAGATGGTTTTGCTTGCAATAATATATTGGCTATTTAAAAAAAAAAATATACATGAAATAGTTTCCATACCTAGCCAAACTATGAAATCTACGTCTTGTGCTAGGCTGCATCTTTTGAAATAATATTATGCTGTTTAAAAAAAAAAAAAAACTCATGCTTTGTTTAAATTTTTGAAATTTTAGGGTTGTCATGGAACACCACAAAGATTGTTTGGACGCCGATTCCAGTGAAGTAGAATACACAGATGATTTATATGATGATGAAGAACATCATAGTTCGTCTGATGCTGAAGATGATGTTGATGGTAATGCTGCTGATGATGTTCATGATGAATCAGTTCAACAAGAGGGTTCAATCAACAATGCATTAGTAGGTGAAAGGTTGGTGAACGTAAATTCCATTGCCTCTGATGAAATTCTTAAACTTGAATTTGGCACTGTTGATGAAGCATATGAATTTTATTATCGTTATGGAAGATGCAAAGGTTTTGCCATTAGGAAAGGTGATGTTAGGAGCAATAGTAGTGGAATAATTACAATGAGAGAGTTTGTGTGCAACAAGCAAGGCTTAAGAGACAAGAAGCACTTATCTAGGAATGATAGAAAAAGAGATCATAAACGTATCACCCGAACAAATTGTGAAGCAAGGCTTCGTGTACGTTACAAAGCCAAAAAGGGGAGATATGTAGTGTCTAAATTTGAAGAGGGGCATAACCATGAATTAACCCCACCTAAATTTACACACTTGCACCCACTGTATCGTAAAATCTCTGAAGCAGATAGTGCCCAACTTGATGCTCTTCAATCACATGGAATCAGAACCTGTCATATAATGGGATATATGGTTGCTCAGAAGGGTGGATACGCTGATGTAGGATTTACTAAGAAAGATCTGTATAACTATTTTGATAAAAAGATGCGTGATGTCATTAAAGACGGTGATGTTGCTGCTGCTCTAAAGCATCTTAATGTGAAGTCTTCCACCGATCCAATGTTATATGCTGAATACGATGTCAACATTGATGGAAGAATGAAAAGTCTGTTTTGGGCTGATGGGAGTAGTAGGTCTGATTATTTTTGTTTTGGCGACGTTTTGGCGTTTGACGCAACGTACAAGAAGAACAAATACAACTATCCGTTGTGTATATTTTCTGGGTGTAACCACCATTCACAGACCATTATATTTGGCGTTGCGTTGTTGGAGGATGAAACAATCGAGTCATATAAGTGGGTCTTGAACCGTTTTTTAGAATGTATGGAAAATAAATTCCCAAAAGCAGTTGTAACAGATGGTGATGGATCAATGAGGGAAGCTATTAAACAGGTATTCCCTGATGCATCTCATCGCTTATGTGCCTGGCATCTGCATAAGAACGCGCAAGAAAATGTAAAGAAAACACCATTTCTGGAGGGTTTTGGAAAAGCAATGTACTCAAATTTTACACCGGAGCAATTTGAGGAGTTTTGGTCAGAGTTAATTCAGAATAATGAACTTGAAGAAAATGATTGGGTTAAGAAGACATACGCGAACAAGTCACTGTGGGCAACCGCATATTTACGTGATAAGTTCTTTGGACGTATTAGAACCACATCTCAATGTGAAGCTATCAATGCAATTGTCAAGACATATGTCAGAGCGAAAGGCAACATTTTTGAATTTATGCATAATTTTGAACAGGTTTTGAGAGATTACAGAAATAGTGAGCTTGCTGCTGAGTTTAAATCAAAATTCACAGAACCTGTGTTGACGACTCACCTGCGTTCGATTGAGAGTGATGCTGCCAAGATTTACACAGCAGAGATCTTTAAAGAAGTTAAAGATGAAATTATTGAGGCTGGTGCAGTAAGTCTCAAGAGCAAAGAATGTGTAGGGGATACCATGGTTTACACATTGCGAAAATATCGTGAGAAAGGCATTGAAAGAGAGGTTGTATACGATGCTGCAAGTTTAGAATTTCAGTGTTCGTGTAGACTATTTGAGACACGTGGGATTCCATGTTGTCATGTTTTTTTTGTGATGAAGGAAGAAGACATTGATCACATACCAAAATGTTTGGTTATGACCCGTTGGACAAAGAACGCAAAAGATGCATTTCTGAACAACGATTCCAATGGGGAAATGGATTCTGATATGATTGAAGTTGCTAGATTTGGTGCTTACTGTTCTGCTTTTACTGCATTCTGTAAAGAAGCCTCAAAAAAGAATGGTGTATTTAGGGACATAATGGATGAAATTTTAAATCTTCAACAAAAGTATTGTGATACTGAATACTCTACTCGGTCTGAGAAATTAACAGATGATCAGGTAGGTGATCCAATCCCTGTGAAGAGTAAAGGTGCTCCAAAGAAGAAGAAGAATGCTACAAAAAGTGTCAGACACTGTTCAAAATGCAGAAGTACAAGTCATACTGCAAGGCATTGTTCGGTAATTTGTCTATATATGATCTGATGAATAATATTTTCACGTAATATGATTTATAATGTGCATGATAAGATATATATATATATATATATATATATATATAAGCAAGTTAATTTTTGCAGGATACACAGAACGGTGAACATGAACATGATGCAGAATTGGTATCTAACTCTATGGATGATTCTTTAAGCCAAAAAGAAAAGGTATGTTTGTTTGCTATGTTTTTACAATGCATCAGAATTACATCTTAACAAAATGTGTATTAATTTATGTCATTCACAGAAAAATAAAAGGAAACCTAATGGTAATGTTAGCACAAGCAAGCAAAAGAAATATAAAGAGGCAGCACAATTTACAGAGATTTCGGAGACAGCTGATTTGCCGGTAACAACTGCAGTTCAACCTACTGTTCATGTGTTCCAGCCTGCTATTAATCCAATGCAAATGCAGCCAATTTACCCCAATTATGGACTTCATGGCGGTGAATCATCAACTTCCTGTTCTGGGCTCTTGGCACAAGTCATGAATTTTGGTGGTCATGGATTACATTTGAATAGTAATTTAGATAAATAA
Protein sequence

>MS.gene067248.t1

MRPTPLPLLSLSCAQPTCDPAAPSSLRPPPRSPSLRVVMEHHKDCLDADSSEVEYTDDLYDDEEHHSSSDAEDDVDGNAADDVHDESVQQEGSINNALVGERLVNVNSIASDEILKLEFGTVDEAYEFYYRYGRCKGFAIRKGDVRSNSSGIITMREFVCNKQGLRDKKHLSRNDRKRDHKRITRTNCEARLRVRYKAKKGRYVVSKFEEGHNHELTPPKFTHLHPLYRKISEADSAQLDALQSHGIRTCHIMGYMVAQKGGYADVGFTKKDLYNYFDKKMRDVIKDGDVAAALKHLNVKSSTDPMLYAEYDVNIDGRMKSLFWADGSSRSDYFCFGDVLAFDATYKKNKYNYPLCIFSGCNHHSQTIIFGVALLEDETIESYKWVLNRFLECMENKFPKAVVTDGDGSMREAIKQVFPDASHRLCAWHLHKNAQENVKKTPFLEGFGKAMYSNFTPEQFEEFWSELIQNNELEENDWVKKTYANKSLWATAYLRDKFFGRIRTTSQCEAINAIVKTYVRAKGNIFEFMHNFEQVLRDYRNSELAAEFKSKFTEPVLTTHLRSIESDAAKIYTAEIFKEVKDEIIEAGAVSLKSKECVGDTMVYTLRKYREKGIEREVVYDAASLEFQCSCRLFETRGIPCCHVFFVMKEEDIDHIPKCLVMTRWTKNAKDAFLNNDSNGEMDSDMIEVARFGAYCSAFTAFCKEASKKNGVFRDIMDEILNLQQKYCDTEYSTRSEKLTDDQVGDPIPVKSKGAPKKKKNATKSVRHCSKCRSTSHTARHCSDTQNGEHEHDAELVSNSMDDSLSQKEKKNKRKPNGNVSTSKQKKYKEAAQFTEISETADLPVTTAVQPTVHVFQPAINPMQMQPIYPNYGLHGGESSTSCSGLLAQVMNFGGHGLHLNSNLDK