AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067315

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067315.t1 MTR_8g024770 92.457 464 25 1 1 464 1 454 0.0 839
MS.gene067315.t1 MTR_8g024790 82.057 457 67 8 13 464 16 462 0.0 744
MS.gene067315.t1 MTR_5g030430 51.980 202 88 4 270 464 474 673 4.38e-54 192
MS.gene067315.t1 MTR_5g030430 33.514 185 90 3 22 177 44 224 3.00e-29 121
MS.gene067315.t1 MTR_8g067280 47.668 193 93 3 271 456 444 635 4.48e-49 178
MS.gene067315.t1 MTR_8g067280 33.005 203 102 7 22 195 53 250 3.75e-26 112
MS.gene067315.t1 MTR_7g096350 27.754 472 239 12 22 436 49 475 8.53e-38 144
MS.gene067315.t1 MTR_5g030420 34.857 175 81 4 22 167 27 197 9.14e-30 116
MS.gene067315.t1 MTR_5g030420 34.857 175 81 4 22 167 27 197 1.08e-29 117
MS.gene067315.t1 MTR_8g027495 38.012 171 92 5 287 456 456 613 2.26e-24 106
MS.gene067315.t1 MTR_7g117670 35.465 172 95 3 287 456 400 557 3.25e-22 100
MS.gene067315.t1 MTR_3g104100 32.927 164 102 3 277 433 183 345 1.05e-17 85.1
MS.gene067315.t1 MTR_1g072320 31.429 210 116 6 253 445 431 629 2.23e-17 85.5
MS.gene067315.t1 MTR_2g081040 33.548 155 95 3 286 433 174 327 5.69e-16 79.7
MS.gene067315.t1 MTR_4g009110 25.989 177 120 3 275 448 164 332 8.36e-16 79.0
MS.gene067315.t1 MTR_4g009540 27.778 180 110 5 275 445 142 310 3.77e-15 76.6
MS.gene067315.t1 MTR_3g030700 35.795 176 98 5 266 433 173 341 5.77e-15 76.6
MS.gene067315.t1 MTR_0250s0040 29.730 148 80 4 260 402 69 197 7.91e-15 74.7
MS.gene067315.t1 MTR_4g097940 29.193 161 99 4 275 429 138 289 8.72e-15 75.5
MS.gene067315.t1 MTR_4g009540 29.268 164 96 5 275 429 142 294 1.23e-14 75.1
MS.gene067315.t1 MTR_7g083900 60.000 55 22 0 287 341 284 338 1.96e-13 72.4
MS.gene067315.t1 MTR_7g083900 60.000 55 22 0 287 341 284 338 2.05e-13 72.4
MS.gene067315.t1 MTR_3g498695 58.182 55 23 0 287 341 296 350 2.14e-13 72.4
MS.gene067315.t1 MTR_3g498695 58.182 55 23 0 287 341 296 350 2.15e-13 72.4
MS.gene067315.t1 MTR_5g014640 31.944 144 83 3 290 429 155 287 2.69e-13 71.2
MS.gene067315.t1 MTR_4g066460 30.263 152 72 5 256 402 139 261 1.52e-12 68.9
MS.gene067315.t1 MTR_4g067010 28.788 132 74 4 276 402 137 253 1.70e-12 68.9
MS.gene067315.t1 MTR_2g022280 27.381 168 105 5 288 442 1 164 2.15e-12 66.2
MS.gene067315.t1 MTR_6g047570 29.323 133 80 3 275 402 145 268 2.51e-12 68.2
MS.gene067315.t1 MTR_4g097950 34.815 135 78 5 274 401 54 185 3.25e-12 67.0
MS.gene067315.t1 MTR_0246s0020 28.814 118 75 2 289 401 198 311 7.16e-12 67.4
MS.gene067315.t1 MTR_4g092700 25.490 204 124 6 272 460 143 333 7.17e-12 67.0
MS.gene067315.t1 MTR_0246s0020 28.814 118 75 2 289 401 198 311 7.48e-12 67.0
MS.gene067315.t1 MTR_4g097920 29.167 168 98 7 268 431 121 271 8.41e-12 66.6
MS.gene067315.t1 MTR_4g097920 29.655 145 87 5 289 431 155 286 8.93e-12 66.6
MS.gene067315.t1 MTR_8g098275 56.364 55 24 0 288 342 243 297 1.02e-11 67.0
MS.gene067315.t1 MTR_2g010450 25.904 166 102 3 289 445 177 330 1.03e-11 66.6
MS.gene067315.t1 MTR_7g110810 31.667 120 71 2 236 345 150 268 1.04e-11 66.6
MS.gene067315.t1 MTR_8g098275 56.364 55 24 0 288 342 307 361 1.14e-11 67.0
MS.gene067315.t1 MTR_0011s0260 29.078 141 96 2 289 429 183 319 1.20e-11 66.2
MS.gene067315.t1 MTR_8g098275 56.364 55 24 0 288 342 400 454 1.26e-11 67.0
MS.gene067315.t1 MTR_2g084280 28.000 150 94 3 293 438 137 276 1.29e-11 65.9
MS.gene067315.t1 MTR_4g094762 30.968 155 93 6 290 434 293 443 1.66e-11 66.6
MS.gene067315.t1 MTR_2g104490 26.630 184 114 8 272 443 116 290 2.09e-11 65.1
MS.gene067315.t1 MTR_0011s0210 27.660 141 98 2 289 429 183 319 2.14e-11 65.5
MS.gene067315.t1 MTR_2g038040 50.000 62 24 1 290 344 53 114 2.31e-11 64.3
MS.gene067315.t1 MTR_2g104550 23.267 202 135 7 249 439 98 290 3.04e-11 64.7
MS.gene067315.t1 MTR_2g104490 26.667 180 111 8 272 439 116 286 3.28e-11 64.7
MS.gene067315.t1 MTR_1g112680 31.092 119 57 2 224 342 277 370 6.26e-11 64.7
MS.gene067315.t1 MTR_5g014600 35.366 82 50 1 269 347 126 207 7.30e-11 63.9
MS.gene067315.t1 MTR_4g057270 52.000 50 24 0 293 342 153 202 9.20e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067315.t1 AT4G00870 35.189 449 217 11 17 455 29 413 5.90e-69 226
MS.gene067315.t1 AT5G46830 32.475 505 252 15 22 464 33 510 3.81e-61 207
MS.gene067315.t1 AT1G32640 49.057 212 90 6 259 464 423 622 1.62e-52 187
MS.gene067315.t1 AT1G32640 31.579 190 93 3 22 178 67 252 8.79e-31 125
MS.gene067315.t1 AT4G17880 48.691 191 83 5 270 455 397 577 5.26e-50 179
MS.gene067315.t1 AT4G17880 30.208 192 94 3 23 178 63 250 1.28e-28 119
MS.gene067315.t1 AT5G46760 47.475 198 96 5 270 464 396 588 5.71e-49 177
MS.gene067315.t1 AT5G46760 32.609 184 88 4 22 173 51 230 1.05e-26 113
MS.gene067315.t1 AT2G46510 27.701 509 251 15 22 442 47 526 1.74e-43 161
MS.gene067315.t1 AT4G16430 26.068 468 262 13 3 437 25 441 2.44e-36 140
MS.gene067315.t1 AT1G01260 38.953 172 78 3 273 444 417 561 1.09e-26 113
MS.gene067315.t1 AT1G01260 38.953 172 78 3 273 444 417 561 1.09e-26 113
MS.gene067315.t1 AT1G01260 38.953 172 78 3 273 444 417 561 1.09e-26 113
MS.gene067315.t1 AT4G00480 21.414 495 278 16 28 436 26 495 1.63e-18 88.6
MS.gene067315.t1 AT4G00480 21.328 497 280 16 28 438 26 497 2.21e-18 88.2
MS.gene067315.t1 AT4G00480 21.328 497 280 16 28 438 26 497 2.32e-18 88.2
MS.gene067315.t1 AT4G37850 29.493 217 121 7 235 429 84 290 5.60e-16 79.3
MS.gene067315.t1 AT4G37850 29.493 217 121 7 235 429 84 290 5.60e-16 79.3
MS.gene067315.t1 AT3G26744 60.000 55 22 0 287 341 305 359 3.40e-14 75.1
MS.gene067315.t1 AT3G26744 60.000 55 22 0 287 341 305 359 3.45e-14 75.1
MS.gene067315.t1 AT3G26744 60.000 55 22 0 287 341 305 359 3.45e-14 75.1
MS.gene067315.t1 AT3G26744 60.000 55 22 0 287 341 305 359 3.45e-14 75.1
MS.gene067315.t1 AT1G12860 60.000 55 22 0 287 341 265 319 4.82e-14 74.3
MS.gene067315.t1 AT1G12860 60.000 55 22 0 287 341 265 319 5.80e-14 74.3
MS.gene067315.t1 AT1G63650 26.061 165 106 4 289 438 405 568 6.35e-13 71.2
MS.gene067315.t1 AT1G63650 26.061 165 106 4 289 438 405 568 6.35e-13 71.2
MS.gene067315.t1 AT1G63650 26.061 165 106 4 289 438 405 568 6.35e-13 71.2
MS.gene067315.t1 AT5G57150 28.977 176 114 5 267 437 38 207 1.07e-12 67.8
MS.gene067315.t1 AT5G57150 28.977 176 114 5 267 437 39 208 1.53e-12 67.4
MS.gene067315.t1 AT5G57150 28.977 176 114 5 267 437 38 207 1.58e-12 67.8
MS.gene067315.t1 AT5G57150 28.977 176 114 5 267 437 39 208 1.66e-12 67.4
MS.gene067315.t1 AT4G29930 62.222 45 17 0 290 334 55 99 2.40e-12 67.0
MS.gene067315.t1 AT5G57150 31.288 163 107 4 290 447 56 218 2.44e-12 67.4
MS.gene067315.t1 AT4G29930 62.222 45 17 0 290 334 55 99 2.60e-12 66.6
MS.gene067315.t1 AT4G29930 62.222 45 17 0 290 334 55 99 2.60e-12 66.6
MS.gene067315.t1 AT4G09820 50.909 55 27 0 288 342 362 416 4.36e-12 68.6
MS.gene067315.t1 AT4G29930 63.636 44 16 0 291 334 56 99 4.92e-12 65.1
MS.gene067315.t1 AT4G29930 62.222 45 17 0 290 334 55 99 5.26e-12 66.6
MS.gene067315.t1 AT4G29930 62.222 45 17 0 290 334 55 99 5.26e-12 66.6
MS.gene067315.t1 AT4G29930 62.222 45 17 0 290 334 55 99 5.27e-12 66.2
MS.gene067315.t1 AT2G16910 29.843 191 107 7 293 461 318 503 5.51e-12 68.2
MS.gene067315.t1 AT3G24140 24.537 216 138 5 269 460 175 389 2.01e-11 66.2
MS.gene067315.t1 AT3G24140 24.537 216 138 5 269 460 175 389 2.62e-11 65.9
MS.gene067315.t1 AT2G22750 36.090 133 76 4 276 401 137 267 4.11e-11 64.7
MS.gene067315.t1 AT5G41315 24.581 179 113 5 280 438 433 609 4.14e-11 65.9
MS.gene067315.t1 AT5G41315 24.581 179 113 5 280 438 441 617 4.21e-11 65.9
MS.gene067315.t1 AT5G41315 24.581 179 113 5 280 438 438 614 4.46e-11 65.5
MS.gene067315.t1 AT2G22750 36.090 133 76 4 276 401 133 263 4.54e-11 64.3
MS.gene067315.t1 AT2G22750 36.090 133 76 4 276 401 137 267 4.61e-11 64.3
MS.gene067315.t1 AT2G22750 36.090 133 76 4 276 401 109 239 5.24e-11 63.9
MS.gene067315.t1 AT2G22750 36.090 133 76 4 276 401 109 239 5.38e-11 63.9

Find 68 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATTGAAGATTTAAAATT+AGG 0.097958 8.4:-70413284 MS.gene067315:CDS
CTTGAAAGATTTGGAGTTTC+AGG 0.118528 8.4:-70413045 MS.gene067315:CDS
CTTCTCATGGGTGGCTTCTT+TGG 0.173047 8.4:+70413634 None:intergenic
GAGATTGATGTGAAGATTAT+AGG 0.182361 8.4:-70413134 MS.gene067315:CDS
TCCAAACCCAATCCGAAAAT+TGG 0.228757 8.4:-70414201 MS.gene067315:CDS
TGAGAATTGCAGACCTAATT+AGG 0.229660 8.4:+70412931 None:intergenic
TTGCTTTAGATTCTGTTCTT+TGG 0.229970 8.4:-70413955 MS.gene067315:CDS
AAGGTAAACCTTTCTTATCT+TGG 0.238310 8.4:-70414141 MS.gene067315:CDS
GTTTGAAGTTGTTGTTTCTT+TGG 0.252021 8.4:+70414096 None:intergenic
ATGACAATATACAACAAAAT+TGG 0.257338 8.4:-70413811 MS.gene067315:CDS
TACTACAACTAGGTTTGTTT+TGG 0.270219 8.4:+70413174 None:intergenic
TCTTCATTTCTCATCTCATT+AGG 0.290165 8.4:+70412957 None:intergenic
AGGTAAACCTTTCTTATCTT+GGG 0.292720 8.4:-70414140 MS.gene067315:CDS
ATCTTAAAGCATAGAATCTA+TGG 0.304209 8.4:+70413387 None:intergenic
AGATTGAAGATTTAAAATTA+GGG 0.310294 8.4:-70413283 MS.gene067315:CDS
AACCATTATAACTTTATTAA+CGG 0.312837 8.4:+70413001 None:intergenic
AACCGTTAATAAAGTTATAA+TGG 0.312996 8.4:-70413003 MS.gene067315:CDS
ATCTAAAGCAAAAGCTTTAC+CGG 0.323817 8.4:+70413967 None:intergenic
TTATCCATCTTTGAAACGTT+TGG 0.324231 8.4:+70413356 None:intergenic
AACTAACGGTGTAATCGAAA+TGG 0.327388 8.4:-70413840 MS.gene067315:CDS
GCCAACATGGGTGTCGTCTC+TGG 0.337682 8.4:-70413665 MS.gene067315:CDS
TTGATTCTGAGGAGGATGTT+TGG 0.339396 8.4:+70413759 None:intergenic
AATGAGTGTCTTGAAAGATT+TGG 0.347886 8.4:-70413054 MS.gene067315:CDS
GGATGTTTGGAACATAGATT+TGG 0.360854 8.4:+70413772 None:intergenic
TCCATGCATGTGTGCTTCAT+TGG 0.364769 8.4:+70413889 None:intergenic
TGTTGAAGGGTTGGTGTTTG+TGG 0.365685 8.4:+70414241 None:intergenic
TGTTGGCAAAAGATGTGTTT+TGG 0.367022 8.4:+70413681 None:intergenic
AGGGTTGGTGTTTGTGGTGA+AGG 0.385693 8.4:+70414247 None:intergenic
TTTATCTCTCCTTTGTCTCT+CGG 0.386285 8.4:+70413415 None:intergenic
TCGATTACACCGTTAGTTGT+AGG 0.388592 8.4:+70413845 None:intergenic
ATGGGTGGCTTCTTTGGCGC+TGG 0.396457 8.4:+70413640 None:intergenic
CAAACAACAAAAGATGAAAA+AGG 0.404302 8.4:-70414160 MS.gene067315:CDS
GGTTCCAAACGTTTCAAAGA+TGG 0.424360 8.4:-70413360 MS.gene067315:CDS
GGGTTGGTGTTTGTGGTGAA+GGG 0.433509 8.4:+70414248 None:intergenic
GACAGTAACACGTGTACAAA+CGG 0.437917 8.4:-70414064 MS.gene067315:CDS
GAAATGAAGAGAGCCTAATT+AGG 0.443372 8.4:-70412944 MS.gene067315:CDS
CCTTTCTTATCTTGGGGTGA+AGG 0.446476 8.4:-70414133 MS.gene067315:CDS
GAGATAATGAAATCTTTCAT+AGG 0.448427 8.4:+70414295 None:intergenic
ACATTTGTCAAGTGGGGTGG+TGG 0.451062 8.4:+70413715 None:intergenic
TTTAAAATTAGGGCAACAAA+AGG 0.456499 8.4:-70413273 MS.gene067315:CDS
AAAAGAGAACCGAAGAAAAG+AGG 0.478932 8.4:-70413485 MS.gene067315:CDS
GTACAAACGGCGATGCTGAA+TGG 0.480446 8.4:-70414051 MS.gene067315:CDS
ACTAACGGTGTAATCGAAAT+GGG 0.485666 8.4:-70413839 MS.gene067315:CDS
TCGGCTTCCACATGGTTCAT+CGG 0.486588 8.4:+70413434 None:intergenic
TTTGGAACATAGATTTGGCT+TGG 0.488416 8.4:+70413777 None:intergenic
CCTTCACCCCAAGATAAGAA+AGG 0.497048 8.4:+70414133 None:intergenic
GGTGTTTGAGTTCGTGTCAA+CGG 0.501817 8.4:+70413455 None:intergenic
TAAATTTGAGTTTGATTCTG+AGG 0.509911 8.4:+70413748 None:intergenic
ATTCTATGCTTTAAGATCTG+TGG 0.527981 8.4:-70413381 MS.gene067315:CDS
ACACTCATTAATCGTGCACT+AGG 0.531276 8.4:+70413068 None:intergenic
AATTGAAGAAGCATTACCTA+TGG 0.532558 8.4:+70413997 None:intergenic
GGTAAACCTTTCTTATCTTG+GGG 0.537701 8.4:-70414139 MS.gene067315:CDS
TTCTTTGTTATCAGATGCAG+TGG 0.539939 8.4:-70413330 MS.gene067315:CDS
TTTGTCTCTCGGCTTCCACA+TGG 0.548386 8.4:+70413426 None:intergenic
TCCAATGAAGCACACATGCA+TGG 0.551566 8.4:-70413890 MS.gene067315:CDS
GCTTCTTCAATTTCATTACC+CGG 0.556623 8.4:-70413986 MS.gene067315:CDS
ATGTGGAAGCCGAGAGACAA+AGG 0.579932 8.4:-70413424 MS.gene067315:CDS
ATTTGAGTTTGATTCTGAGG+AGG 0.594452 8.4:+70413751 None:intergenic
ATTTGTATACCTACAACTAA+CGG 0.599891 8.4:-70413854 MS.gene067315:CDS
ATAAGATTCTTACTACAACT+AGG 0.600771 8.4:+70413164 None:intergenic
TTTACATTTGTCAAGTGGGG+TGG 0.601476 8.4:+70413712 None:intergenic
GCCAGAGACGACACCCATGT+TGG 0.603838 8.4:+70413664 None:intergenic
GATTATAGGACATGATGCTA+TGG 0.615350 8.4:-70413120 MS.gene067315:CDS
CACTCATTAATCGTGCACTA+GGG 0.626623 8.4:+70413069 None:intergenic
TAGATTTGGCTTGGTATACG+AGG 0.640872 8.4:+70413786 None:intergenic
TCTAAAGCAAAAGCTTTACC+GGG 0.640910 8.4:+70413968 None:intergenic
TGCAACGACAACTTCAATCG+TGG 0.678130 8.4:-70413201 MS.gene067315:CDS
TCAAACACCGATGAACCATG+TGG 0.723026 8.4:-70413441 MS.gene067315:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACCATTATAACTTTATTAA+CGG + chr8.4:70414214-70414233 None:intergenic 15.0%
!!! AAGATTGAAGATTTAAAATT+AGG - chr8.4:70413928-70413947 MS.gene067315:CDS 15.0%
!!! AGATTGAAGATTTAAAATTA+GGG - chr8.4:70413929-70413948 MS.gene067315:CDS 15.0%
!! AACCGTTAATAAAGTTATAA+TGG - chr8.4:70414209-70414228 MS.gene067315:CDS 20.0%
!! ATGACAATATACAACAAAAT+TGG - chr8.4:70413401-70413420 MS.gene067315:CDS 20.0%
! ATAAGATTCTTACTACAACT+AGG + chr8.4:70414051-70414070 None:intergenic 25.0%
! ATCTTAAAGCATAGAATCTA+TGG + chr8.4:70413828-70413847 None:intergenic 25.0%
! ATTTGTATACCTACAACTAA+CGG - chr8.4:70413358-70413377 MS.gene067315:CDS 25.0%
! CAAACAACAAAAGATGAAAA+AGG - chr8.4:70413052-70413071 MS.gene067315:CDS 25.0%
!! AATGTTTTACATTTGTCAAG+TGG + chr8.4:70413508-70413527 None:intergenic 25.0%
!! ATGTTTTACATTTGTCAAGT+GGG + chr8.4:70413507-70413526 None:intergenic 25.0%
!! TAAATTTGAGTTTGATTCTG+AGG + chr8.4:70413467-70413486 None:intergenic 25.0%
!! TTTAAAATTAGGGCAACAAA+AGG - chr8.4:70413939-70413958 MS.gene067315:CDS 25.0%
!!! AAATTTGAAGCTTTTGTTGA+AGG + chr8.4:70412988-70413007 None:intergenic 25.0%
!!! AATTTGAAGCTTTTGTTGAA+GGG + chr8.4:70412987-70413006 None:intergenic 25.0%
!!! CAATTTTTTTTGCTTCTCAT+GGG + chr8.4:70413593-70413612 None:intergenic 25.0%
AAGGTAAACCTTTCTTATCT+TGG - chr8.4:70413071-70413090 MS.gene067315:CDS 30.0%
AATGAGTGTCTTGAAAGATT+TGG - chr8.4:70414158-70414177 MS.gene067315:CDS 30.0%
AGGTAAACCTTTCTTATCTT+GGG - chr8.4:70413072-70413091 MS.gene067315:CDS 30.0%
GAGATTGATGTGAAGATTAT+AGG - chr8.4:70414078-70414097 MS.gene067315:CDS 30.0%
TCTTCATTTCTCATCTCATT+AGG + chr8.4:70414258-70414277 None:intergenic 30.0%
! AATGGCATAGATCCAATTTT+CGG + chr8.4:70413026-70413045 None:intergenic 30.0%
! ATAGATCCAATTTTCGGATT+GGG + chr8.4:70413020-70413039 None:intergenic 30.0%
! ATCTAAAGCAAAAGCTTTAC+CGG + chr8.4:70413248-70413267 None:intergenic 30.0%
! ATGACTCGAAATTTTTCCAT+AGG - chr8.4:70413199-70413218 MS.gene067315:CDS 30.0%
! TGTTTTACATTTGTCAAGTG+GGG + chr8.4:70413506-70413525 None:intergenic 30.0%
! TTGCTTTAGATTCTGTTCTT+TGG - chr8.4:70413257-70413276 MS.gene067315:CDS 30.0%
! TTTTCATGAGAAGATGAAGA+TGG + chr8.4:70412944-70412963 None:intergenic 30.0%
!! AATTGAAGAAGCATTACCTA+TGG + chr8.4:70413218-70413237 None:intergenic 30.0%
!! ATTCTATGCTTTAAGATCTG+TGG - chr8.4:70413831-70413850 MS.gene067315:CDS 30.0%
!! GTTTGAAGTTGTTGTTTCTT+TGG + chr8.4:70413119-70413138 None:intergenic 30.0%
!! TACTACAACTAGGTTTGTTT+TGG + chr8.4:70414041-70414060 None:intergenic 30.0%
!! TTATCCATCTTTGAAACGTT+TGG + chr8.4:70413859-70413878 None:intergenic 30.0%
!!! GCAATTTTTTTTGCTTCTCA+TGG + chr8.4:70413594-70413613 None:intergenic 30.0%
!!! GTTGTTTTTGTTGCTAATAG+TGG + chr8.4:70413682-70413701 None:intergenic 30.0%
!!! TTTTGTTGTTTGCCACAAAA+TGG + chr8.4:70413044-70413063 None:intergenic 30.0%
AAAAGAGAACCGAAGAAAAG+AGG - chr8.4:70413727-70413746 MS.gene067315:CDS 35.0%
AACTAACGGTGTAATCGAAA+TGG - chr8.4:70413372-70413391 MS.gene067315:CDS 35.0%
ACTAACGGTGTAATCGAAAT+GGG - chr8.4:70413373-70413392 MS.gene067315:CDS 35.0%
GAAATGAAGAGAGCCTAATT+AGG - chr8.4:70414268-70414287 MS.gene067315:CDS 35.0%
GATTATAGGACATGATGCTA+TGG - chr8.4:70414092-70414111 MS.gene067315:CDS 35.0%
GCTTCTTCAATTTCATTACC+CGG - chr8.4:70413226-70413245 MS.gene067315:CDS 35.0%
GGTAAACCTTTCTTATCTTG+GGG - chr8.4:70413073-70413092 MS.gene067315:CDS 35.0%
TGAGAATTGCAGACCTAATT+AGG + chr8.4:70414284-70414303 None:intergenic 35.0%
TTTATCTCTCCTTTGTCTCT+CGG + chr8.4:70413800-70413819 None:intergenic 35.0%
! AAACACATCTTTTGCCAACA+TGG - chr8.4:70413534-70413553 MS.gene067315:CDS 35.0%
! AACACATCTTTTGCCAACAT+GGG - chr8.4:70413535-70413554 MS.gene067315:CDS 35.0%
! ATTTGAGTTTGATTCTGAGG+AGG + chr8.4:70413464-70413483 None:intergenic 35.0%
! CATAGATCCAATTTTCGGAT+TGG + chr8.4:70413021-70413040 None:intergenic 35.0%
! CTTGAAAGATTTGGAGTTTC+AGG - chr8.4:70414167-70414186 MS.gene067315:CDS 35.0%
! GGATGTTTGGAACATAGATT+TGG + chr8.4:70413443-70413462 None:intergenic 35.0%
! GGTTTTCTTCCTCTTTTCTT+CGG + chr8.4:70413739-70413758 None:intergenic 35.0%
! TCTAAAGCAAAAGCTTTACC+GGG + chr8.4:70413247-70413266 None:intergenic 35.0%
! TGTTGGCAAAAGATGTGTTT+TGG + chr8.4:70413534-70413553 None:intergenic 35.0%
! TTCTTTGTTATCAGATGCAG+TGG - chr8.4:70413882-70413901 MS.gene067315:CDS 35.0%
! TTTGGAACATAGATTTGGCT+TGG + chr8.4:70413438-70413457 None:intergenic 35.0%
!!! ATTGGATCTATGCCATTTTG+TGG - chr8.4:70413029-70413048 MS.gene067315:CDS 35.0%
!!! TTTTTTTTGCTTCTCATGGG+TGG + chr8.4:70413590-70413609 None:intergenic 35.0%
ACACTCATTAATCGTGCACT+AGG + chr8.4:70414147-70414166 None:intergenic 40.0%
CACTCATTAATCGTGCACTA+GGG + chr8.4:70414146-70414165 None:intergenic 40.0%
GACAGTAACACGTGTACAAA+CGG - chr8.4:70413148-70413167 MS.gene067315:CDS 40.0%
GGTTCCAAACGTTTCAAAGA+TGG - chr8.4:70413852-70413871 MS.gene067315:CDS 40.0%
TCCAAACCCAATCCGAAAAT+TGG - chr8.4:70413011-70413030 MS.gene067315:CDS 40.0%
TCGATTACACCGTTAGTTGT+AGG + chr8.4:70413370-70413389 None:intergenic 40.0%
TTTACATTTGTCAAGTGGGG+TGG + chr8.4:70413503-70413522 None:intergenic 40.0%
! TGAAGCTTTTGTTGAAGGGT+TGG + chr8.4:70412983-70413002 None:intergenic 40.0%
! TTGATTCTGAGGAGGATGTT+TGG + chr8.4:70413456-70413475 None:intergenic 40.0%
!! TAGATTTGGCTTGGTATACG+AGG + chr8.4:70413429-70413448 None:intergenic 40.0%
!! TCCAATTTTCGGATTGGGTT+TGG + chr8.4:70413015-70413034 None:intergenic 40.0%
CCTTCACCCCAAGATAAGAA+AGG + chr8.4:70413082-70413101 None:intergenic 45.0%
CCTTTCTTATCTTGGGGTGA+AGG - chr8.4:70413079-70413098 MS.gene067315:CDS 45.0%
TCAAACACCGATGAACCATG+TGG - chr8.4:70413771-70413790 MS.gene067315:CDS 45.0%
TCCAATGAAGCACACATGCA+TGG - chr8.4:70413322-70413341 MS.gene067315:CDS 45.0%
TCCATGCATGTGTGCTTCAT+TGG + chr8.4:70413326-70413345 None:intergenic 45.0%
TGCAACGACAACTTCAATCG+TGG - chr8.4:70414011-70414030 MS.gene067315:CDS 45.0%
! GGTGTTTGAGTTCGTGTCAA+CGG + chr8.4:70413760-70413779 None:intergenic 45.0%
!! TGGGGTGAAGGCTATTTTCA+AGG - chr8.4:70413091-70413110 MS.gene067315:CDS 45.0%
!! TGTTGAAGGGTTGGTGTTTG+TGG + chr8.4:70412974-70412993 None:intergenic 45.0%
ACATTTGTCAAGTGGGGTGG+TGG + chr8.4:70413500-70413519 None:intergenic 50.0%
ATGTGGAAGCCGAGAGACAA+AGG - chr8.4:70413788-70413807 MS.gene067315:CDS 50.0%
CTTCTCATGGGTGGCTTCTT+TGG + chr8.4:70413581-70413600 None:intergenic 50.0%
GTACAAACGGCGATGCTGAA+TGG - chr8.4:70413161-70413180 MS.gene067315:CDS 50.0%
TCGGCTTCCACATGGTTCAT+CGG + chr8.4:70413781-70413800 None:intergenic 50.0%
TTTGTCTCTCGGCTTCCACA+TGG + chr8.4:70413789-70413808 None:intergenic 50.0%
!! AGGGTTGGTGTTTGTGGTGA+AGG + chr8.4:70412968-70412987 None:intergenic 50.0%
!! GGGTTGGTGTTTGTGGTGAA+GGG + chr8.4:70412967-70412986 None:intergenic 50.0%
ATGGGTGGCTTCTTTGGCGC+TGG + chr8.4:70413575-70413594 None:intergenic 60.0%
GCCAGAGACGACACCCATGT+TGG + chr8.4:70413551-70413570 None:intergenic 60.0%
! GCCAACATGGGTGTCGTCTC+TGG - chr8.4:70413547-70413566 MS.gene067315:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 70412920 70414314 70412920 ID=MS.gene067315
chr8.4 mRNA 70412920 70414314 70412920 ID=MS.gene067315.t1;Parent=MS.gene067315
chr8.4 exon 70412920 70414314 70412920 ID=MS.gene067315.t1.exon1;Parent=MS.gene067315.t1
chr8.4 CDS 70412920 70414314 70412920 ID=cds.MS.gene067315.t1;Parent=MS.gene067315.t1
Gene Sequence

>MS.gene067315

ATGAAAGATTTCATTATCTCACCATCTTCATCTTCTCATGAAAACCCTTCACCACAAACACCAACCCTTCAACAAAAGCTTCAAATTTTAGTCCAAACCCAATCCGAAAATTGGATCTATGCCATTTTGTGGCAAACAACAAAAGATGAAAAAGGTAAACCTTTCTTATCTTGGGGTGAAGGCTATTTTCAAGGAACCAAAGAAACAACAACTTCAAACAAACAAAACGACAGTAACACGTGTACAAACGGCGATGCTGAATGGTTCTACATGATGTCGATGACTCGAAATTTTTCCATAGGTAATGCTTCTTCAATTTCATTACCCGGTAAAGCTTTTGCTTTAGATTCTGTTCTTTGGTTGAATAGTAAACATGAACTTCAAATTTATAACTGTGAGCGATCCAATGAAGCACACATGCATGGAATTGAAACACTGATTTGTATACCTACAACTAACGGTGTAATCGAAATGGGTTCTTATGACAATATACAACAAAATTGGAACCTCGTATACCAAGCCAAATCTATGTTCCAAACATCCTCCTCAGAATCAAACTCAAATTTAGATCTTAACTCCACCACCCCACTTGACAAATGTAAAACATTCATCCAAAACACATCTTTTGCCAACATGGGTGTCGTCTCTGGCGCCAGCGCCAAAGAAGCCACCCATGAGAAGCAAAAAAAATTGCAAGAAAAACATAAAAATGTTTCATCATGTTCTTTAGATTCTGAAGATTCTGATTCAGAATATTGTCCACTATTAGCAACAAAAACAACAACGACAAAGAACGATTCTTTTGAGAAAAGAGAACCGAAGAAAAGAGGAAGAAAACCGTTGACACGAACTCAAACACCGATGAACCATGTGGAAGCCGAGAGACAAAGGAGAGATAAACTAAACCATAGATTCTATGCTTTAAGATCTGTGGTTCCAAACGTTTCAAAGATGGATAAAGCTTCTTTGTTATCAGATGCAGTGGATTACATCAATGAGTTAAAAGCAAAGATTGAAGATTTAAAATTAGGGCAACAAAAGGAATCGAAAAAGATGATGATGAAAACGATGAAAATTGTCGATAATAACAGTGCAACGACAACTTCAATCGTGGTAGACCAAAACAAACCTAGTTGTAGTAAGAATCTTATTGCTTTAGAGATTGATGTGAAGATTATAGGACATGATGCTATGGTGAGAGTTCAAAGTTTGAATGTTAATCACCCTAGTGCACGATTAATGAGTGTCTTGAAAGATTTGGAGTTTCAGGTACATCATGCTAGTATATCAACCGTTAATAAAGTTATAATGGTTCAAGATGTTATTGTTAAAATTCCTAATGAGATGAGAAATGAAGAGAGCCTAATTAGGTCTGCAATTCTCATGAAATTATGA
Protein sequence

>MS.gene067315.t1

MKDFIISPSSSSHENPSPQTPTLQQKLQILVQTQSENWIYAILWQTTKDEKGKPFLSWGEGYFQGTKETTTSNKQNDSNTCTNGDAEWFYMMSMTRNFSIGNASSISLPGKAFALDSVLWLNSKHELQIYNCERSNEAHMHGIETLICIPTTNGVIEMGSYDNIQQNWNLVYQAKSMFQTSSSESNSNLDLNSTTPLDKCKTFIQNTSFANMGVVSGASAKEATHEKQKKLQEKHKNVSSCSLDSEDSDSEYCPLLATKTTTTKNDSFEKREPKKRGRKPLTRTQTPMNHVEAERQRRDKLNHRFYALRSVVPNVSKMDKASLLSDAVDYINELKAKIEDLKLGQQKESKKMMMKTMKIVDNNSATTTSIVVDQNKPSCSKNLIALEIDVKIIGHDAMVRVQSLNVNHPSARLMSVLKDLEFQVHHASISTVNKVIMVQDVIVKIPNEMRNEESLIRSAILMKL