AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067374

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067374.t1 MTR_5g027440 93.885 278 16 1 1 277 1 278 0.0 538
MS.gene067374.t1 MTR_6g444980 52.439 164 63 4 10 171 22 172 3.47e-51 172
MS.gene067374.t1 MTR_2g086450 49.744 195 72 4 18 194 38 224 6.20e-51 172
MS.gene067374.t1 MTR_6g032990 50.777 193 85 4 18 204 35 223 2.24e-50 171
MS.gene067374.t1 MTR_7g088070 54.545 143 52 1 25 167 50 179 2.52e-48 162
MS.gene067374.t1 MTR_6g444980 49.153 177 75 4 10 184 22 185 5.99e-48 163
MS.gene067374.t1 MTR_7g088070 55.245 143 55 2 25 167 50 183 4.97e-47 159
MS.gene067374.t1 MTR_4g081710 51.592 157 69 3 18 171 10 162 6.37e-45 155
MS.gene067374.t1 MTR_2g027860 39.526 253 127 5 25 270 49 282 9.45e-45 154
MS.gene067374.t1 MTR_5g041350 50.000 156 72 3 19 171 9 161 1.45e-44 154
MS.gene067374.t1 MTR_2g027800 47.590 166 85 2 7 171 20 184 5.97e-43 149
MS.gene067374.t1 MTR_1g090670 50.000 154 73 2 18 171 37 186 2.23e-42 147
MS.gene067374.t1 MTR_2g027800 48.193 166 70 4 7 171 20 170 6.87e-41 143
MS.gene067374.t1 MTR_7g098250 49.645 141 59 2 25 165 262 390 5.46e-36 134
MS.gene067374.t1 MTR_7g115530 44.785 163 78 2 25 187 218 368 8.29e-36 133
MS.gene067374.t1 MTR_1g080330 45.270 148 69 2 18 165 168 303 1.44e-33 126
MS.gene067374.t1 MTR_1g053835 43.448 145 68 1 25 169 258 388 3.08e-33 125
MS.gene067374.t1 MTR_7g093030 41.497 147 73 4 25 171 212 345 9.47e-30 115
MS.gene067374.t1 MTR_1g080330 45.669 127 57 2 18 144 168 282 5.52e-27 106
MS.gene067374.t1 MTR_7g115530 46.667 120 52 2 25 144 218 325 5.31e-26 104
MS.gene067374.t1 MTR_7g068600 39.860 143 67 3 25 162 13 141 7.34e-25 99.8
MS.gene067374.t1 MTR_7g115530 58.462 65 27 0 25 89 218 282 4.79e-21 90.9
MS.gene067374.t1 MTR_1g053800 32.558 172 98 2 12 178 159 317 1.18e-20 90.1
MS.gene067374.t1 MTR_7g115530 57.812 64 27 0 25 88 218 281 5.74e-20 88.6
MS.gene067374.t1 MTR_7g093030 57.895 57 24 0 25 81 212 268 8.57e-20 87.0
MS.gene067374.t1 MTR_2g023580 46.939 98 50 1 16 113 46 141 6.73e-19 83.6
MS.gene067374.t1 MTR_1g053830 55.357 56 25 0 27 82 20 75 7.99e-19 79.7
MS.gene067374.t1 MTR_1g112370 47.059 102 42 3 1 97 1 95 1.68e-18 84.3
MS.gene067374.t1 MTR_7g069660 62.069 58 22 0 21 78 67 124 2.91e-18 83.6
MS.gene067374.t1 MTR_0223s0040 58.209 67 28 0 12 78 5 71 4.22e-18 82.4
MS.gene067374.t1 MTR_7g115530 65.385 52 18 0 25 76 218 269 6.29e-18 81.6
MS.gene067374.t1 MTR_6g071625 61.667 60 23 0 26 85 203 262 2.09e-17 81.3
MS.gene067374.t1 MTR_8g066310 69.811 53 16 0 26 78 174 226 2.89e-17 80.9
MS.gene067374.t1 MTR_7g115530 64.706 51 18 0 25 75 218 268 3.66e-17 79.7
MS.gene067374.t1 MTR_2g084230 52.941 68 32 0 11 78 83 150 1.01e-16 79.0
MS.gene067374.t1 MTR_5g029470 69.811 53 16 0 26 78 220 272 1.01e-16 79.7
MS.gene067374.t1 MTR_1g021520 67.925 53 17 0 26 78 127 179 1.06e-16 78.6
MS.gene067374.t1 MTR_2g084230 52.941 68 32 0 11 78 83 150 1.14e-16 79.3
MS.gene067374.t1 MTR_8g086410 51.389 72 28 1 7 78 100 164 1.31e-16 79.3
MS.gene067374.t1 MTR_8g086410 51.389 72 28 1 7 78 98 162 1.36e-16 79.3
MS.gene067374.t1 MTR_3g450310 48.611 72 30 1 7 78 8 72 2.14e-16 76.3
MS.gene067374.t1 MTR_3g064840 67.925 53 17 0 26 78 275 327 2.71e-16 78.6
MS.gene067374.t1 MTR_1g090670 40.870 115 64 2 57 171 1 111 6.80e-16 75.1
MS.gene067374.t1 MTR_2g016220 49.333 75 38 0 4 78 68 142 1.11e-15 76.6
MS.gene067374.t1 MTR_2g016220 49.333 75 38 0 4 78 68 142 1.15e-15 76.6
MS.gene067374.t1 MTR_7g093010 36.486 148 74 4 25 168 213 344 1.74e-15 75.9
MS.gene067374.t1 MTR_8g086410 48.611 72 30 1 7 78 100 164 2.65e-15 75.9
MS.gene067374.t1 MTR_2g016220 48.611 72 37 0 4 75 68 139 3.43e-14 72.0
MS.gene067374.t1 MTR_4g121020 58.182 55 22 1 25 79 111 164 7.14e-13 68.6
MS.gene067374.t1 MTR_4g121020 58.182 55 22 1 25 79 215 268 8.38e-13 68.6
MS.gene067374.t1 MTR_4g121020 58.182 55 22 1 25 79 215 268 8.54e-13 68.6
MS.gene067374.t1 MTR_5g017980 37.143 105 63 2 25 128 193 295 4.57e-12 65.5
MS.gene067374.t1 MTR_7g089010 56.364 55 23 1 25 79 91 144 6.15e-12 65.1
MS.gene067374.t1 MTR_4g113140 54.098 61 26 1 25 85 235 293 7.43e-12 65.5
MS.gene067374.t1 MTR_2g450070 52.727 55 25 1 25 79 213 266 7.65e-12 65.9
MS.gene067374.t1 MTR_2g450070 52.727 55 25 1 25 79 213 266 8.37e-12 65.5
MS.gene067374.t1 MTR_1g093080 50.820 61 28 1 25 85 211 269 1.07e-11 64.7
MS.gene067374.t1 MTR_4g064730 54.545 55 24 1 25 79 131 184 1.46e-11 63.9
MS.gene067374.t1 MTR_1g032570 52.727 55 25 1 25 79 131 184 1.50e-11 64.7
MS.gene067374.t1 MTR_1g032570 52.727 55 25 1 25 79 200 253 1.87e-11 64.7
MS.gene067374.t1 MTR_4g086835 54.098 61 26 1 25 85 274 332 2.01e-11 64.3
MS.gene067374.t1 MTR_4g021790 34.653 101 62 3 25 125 168 264 2.16e-11 63.2
MS.gene067374.t1 MTR_1g013170 54.237 59 26 1 21 79 187 244 3.21e-11 63.5
MS.gene067374.t1 MTR_5g041350 60.417 48 19 0 124 171 3 50 8.73e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067374.t1 AT5G45580 50.174 287 121 11 2 274 1 279 2.45e-76 234
MS.gene067374.t1 AT5G45580 50.929 269 111 10 20 274 2 263 2.45e-74 228
MS.gene067374.t1 AT3G24120 50.829 181 87 1 16 194 32 212 1.69e-57 186
MS.gene067374.t1 AT4G13640 50.286 175 85 1 16 188 28 202 1.08e-56 184
MS.gene067374.t1 AT3G24120 50.000 184 87 2 16 194 32 215 3.03e-55 180
MS.gene067374.t1 AT4G13640 49.438 178 85 2 16 188 28 205 1.82e-54 178
MS.gene067374.t1 AT3G04030 48.718 195 87 5 7 193 29 218 6.58e-52 174
MS.gene067374.t1 AT5G18240 48.187 193 91 4 7 193 29 218 1.35e-50 171
MS.gene067374.t1 AT5G18240 48.187 193 91 4 7 193 29 218 1.35e-50 171
MS.gene067374.t1 AT3G04030 47.692 195 88 6 7 193 29 217 7.44e-49 166
MS.gene067374.t1 AT1G79430 40.517 232 98 5 10 217 21 236 5.96e-48 163
MS.gene067374.t1 AT3G12730 47.273 165 69 4 18 173 16 171 1.07e-47 159
MS.gene067374.t1 AT5G18240 47.150 193 91 5 7 193 29 216 3.53e-47 162
MS.gene067374.t1 AT3G04030 46.667 195 85 6 7 193 29 212 4.82e-47 162
MS.gene067374.t1 AT5G18240 46.114 193 89 5 7 193 29 212 1.16e-45 158
MS.gene067374.t1 AT5G18240 46.114 193 89 5 7 193 29 212 1.16e-45 158
MS.gene067374.t1 AT1G69580 49.398 166 79 2 18 178 27 192 6.09e-45 155
MS.gene067374.t1 AT1G69580 48.503 167 80 2 18 178 27 193 3.49e-44 153
MS.gene067374.t1 AT2G01060 54.545 143 54 2 25 167 15 146 7.13e-44 150
MS.gene067374.t1 AT3G13040 46.250 160 76 2 25 181 241 393 1.48e-37 138
MS.gene067374.t1 AT3G13040 46.250 160 76 2 25 181 241 393 1.48e-37 138
MS.gene067374.t1 AT3G13040 46.250 160 76 2 25 181 241 393 1.48e-37 138
MS.gene067374.t1 AT4G28610 43.750 160 78 2 25 184 225 372 7.96e-35 130
MS.gene067374.t1 AT3G04450 44.521 146 66 2 25 167 198 331 7.16e-33 124
MS.gene067374.t1 AT3G04450 44.521 146 66 2 25 167 198 331 7.16e-33 124
MS.gene067374.t1 AT3G04450 44.521 146 66 2 25 167 238 371 9.47e-33 125
MS.gene067374.t1 AT5G06800 44.056 143 72 3 25 167 192 326 1.08e-32 124
MS.gene067374.t1 AT5G06800 44.755 143 66 3 25 167 192 321 2.20e-32 122
MS.gene067374.t1 AT5G29000 44.828 145 67 2 25 167 188 321 2.49e-32 122
MS.gene067374.t1 AT5G29000 44.828 145 67 2 25 167 188 321 2.49e-32 122
MS.gene067374.t1 AT5G29000 44.828 145 67 2 25 167 231 364 4.29e-32 122
MS.gene067374.t1 AT5G45580 39.815 216 109 10 73 274 16 224 2.06e-31 116
MS.gene067374.t1 AT3G04030 46.897 145 66 4 57 193 1 142 2.68e-29 113
MS.gene067374.t1 AT2G20400 43.165 139 68 1 27 165 233 360 2.48e-27 109
MS.gene067374.t1 AT2G20400 43.165 139 67 2 27 165 233 359 2.63e-26 106
MS.gene067374.t1 AT3G04030 44.138 145 64 5 57 193 1 136 1.40e-24 100
MS.gene067374.t1 AT5G18240 42.657 143 69 4 57 193 1 136 7.38e-24 99.0
MS.gene067374.t1 AT2G06020 39.597 149 61 5 3 130 58 198 7.10e-23 95.9
MS.gene067374.t1 AT2G01060 48.148 108 45 2 60 167 1 97 2.39e-22 93.2
MS.gene067374.t1 AT1G79430 44.167 120 48 3 57 171 1 106 6.31e-22 93.2
MS.gene067374.t1 AT3G12730 38.889 126 59 4 57 173 1 117 5.81e-21 87.8
MS.gene067374.t1 AT5G06800 54.688 64 29 0 25 88 192 255 7.41e-20 87.0
MS.gene067374.t1 AT2G42660 69.811 53 16 0 26 78 51 103 3.59e-19 84.7
MS.gene067374.t1 AT4G04605 55.556 63 28 0 16 78 5 67 6.74e-19 83.2
MS.gene067374.t1 AT2G02060 51.765 85 37 1 1 81 1 85 8.29e-19 84.0
MS.gene067374.t1 AT2G02060 51.765 85 37 1 1 81 1 85 8.29e-19 84.0
MS.gene067374.t1 AT5G29000 53.623 69 32 0 25 93 231 299 1.66e-18 84.0
MS.gene067374.t1 AT1G14600 56.000 75 31 1 6 78 2 76 2.74e-18 82.4
MS.gene067374.t1 AT3G04450 56.897 58 25 0 25 82 238 295 4.52e-18 82.8
MS.gene067374.t1 AT2G40260 57.812 64 27 0 15 78 72 135 5.14e-18 83.6
MS.gene067374.t1 AT4G04580 46.250 80 38 1 14 93 3 77 4.16e-17 77.0
MS.gene067374.t1 AT1G32240 69.811 53 16 0 26 78 214 266 5.58e-17 80.1
MS.gene067374.t1 AT1G32240 69.811 53 16 0 26 78 214 266 7.72e-17 80.1
MS.gene067374.t1 AT5G16560 67.925 53 17 0 26 78 220 272 9.97e-17 79.7
MS.gene067374.t1 AT5G42630 66.038 53 18 0 26 78 106 158 1.13e-16 77.4
MS.gene067374.t1 AT2G38300 61.404 57 22 0 22 78 51 107 1.40e-16 79.0
MS.gene067374.t1 AT5G42630 66.038 53 18 0 26 78 106 158 2.75e-16 77.4
MS.gene067374.t1 AT4G17695 66.038 53 18 0 26 78 165 217 5.04e-16 77.0
MS.gene067374.t1 AT4G37180 55.932 59 24 1 27 85 219 275 3.51e-13 69.3
MS.gene067374.t1 AT4G37180 55.932 59 24 1 27 85 212 268 3.60e-13 69.3
MS.gene067374.t1 AT3G25790 39.286 112 51 4 30 128 201 308 6.37e-13 68.6
MS.gene067374.t1 AT3G10760 51.351 74 31 3 6 79 90 158 8.42e-13 67.8
MS.gene067374.t1 AT1G49560 46.988 83 42 1 25 107 192 272 2.11e-12 66.6
MS.gene067374.t1 AT4G04555 46.032 63 34 0 16 78 5 67 2.23e-12 62.8
MS.gene067374.t1 AT5G05090 56.364 55 23 1 25 79 81 134 2.62e-12 65.9
MS.gene067374.t1 AT3G46640 54.545 55 24 1 25 79 144 197 6.08e-12 65.5
MS.gene067374.t1 AT3G46640 54.545 55 24 1 25 79 144 197 6.08e-12 65.5
MS.gene067374.t1 AT5G59570 54.545 55 24 1 25 79 141 194 6.34e-12 65.1
MS.gene067374.t1 AT5G59570 54.545 55 24 1 25 79 141 194 6.34e-12 65.1
MS.gene067374.t1 AT3G46640 54.545 55 24 1 25 79 144 197 6.85e-12 65.1
MS.gene067374.t1 AT5G58080 51.515 66 29 2 25 90 194 256 7.03e-12 65.9
MS.gene067374.t1 AT2G40970 56.364 55 23 1 25 79 105 158 1.05e-11 63.9
MS.gene067374.t1 AT2G03500 54.098 61 26 1 25 85 233 291 2.53e-11 63.9
MS.gene067374.t1 AT3G16857 52.727 55 25 1 25 79 167 220 4.12e-11 63.5
MS.gene067374.t1 AT1G13300 50.820 61 28 1 25 85 181 239 4.19e-11 63.2
MS.gene067374.t1 AT3G16857 52.727 55 25 1 25 79 237 290 4.78e-11 63.5
MS.gene067374.t1 AT3G16857 52.727 55 25 1 25 79 237 290 5.01e-11 63.2
MS.gene067374.t1 AT2G01760 52.727 55 25 1 25 79 135 188 7.27e-11 62.0

Find 67 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAGCTTTGTCAAATTTCA+TGG 0.192891 5.2:-18174075 MS.gene067374:CDS
TGCCTTAGTGCTTCTCCTAT+TGG 0.203121 5.2:+18174661 None:intergenic
ATTAACCTTAGAACAGATTT+AGG 0.207865 5.2:+18175589 None:intergenic
TAAATCTGTTCTAAGGTTAA+TGG 0.217308 5.2:-18175587 MS.gene067374:CDS
GCCTTAGTGCTTCTCCTATT+GGG 0.237177 5.2:+18174662 None:intergenic
CTAAGGTTAATGGGATTAAA+AGG 0.242641 5.2:-18175577 MS.gene067374:CDS
GTCCACCTCAACCTCGGCTT+CGG 0.264907 5.2:+18176033 None:intergenic
AGAGGCTCAAGGGAAGTATT+TGG 0.284186 5.2:-18174186 MS.gene067374:CDS
CAGCTAGACTTACACATTAA+AGG 0.298635 5.2:-18173906 MS.gene067374:CDS
TCATTTGAAGAGCCATTTAC+AGG 0.303807 5.2:-18175542 MS.gene067374:intron
AATTTCAGTAACCATTCTTC+AGG 0.305474 5.2:-18174945 MS.gene067374:CDS
AAATCTGTTCTAAGGTTAAT+GGG 0.321195 5.2:-18175586 MS.gene067374:CDS
ATCCAATATGGTTTCATATA+GGG 0.323553 5.2:-18173844 MS.gene067374:CDS
AATCCAATATGGTTTCATAT+AGG 0.356297 5.2:-18173845 MS.gene067374:CDS
GGAGGGAGAGGAGGAATTGA+AGG 0.362824 5.2:-18176090 MS.gene067374:CDS
ACTAGGGACAGAAGAAAATA+AGG 0.364063 5.2:-18173961 MS.gene067374:CDS
TAGAAGTTGTTACCTGTAAA+TGG 0.368392 5.2:+18175530 None:intergenic
TAATGCATCAAAAGCTCAAT+TGG 0.371170 5.2:-18174108 MS.gene067374:CDS
AATTGAAGTTCAAAAGAGAT+TGG 0.388648 5.2:-18174635 MS.gene067374:CDS
AGCTGAAATGGAATCCAATA+TGG 0.390326 5.2:-18173856 MS.gene067374:CDS
TACCCTATATGAAACCATAT+TGG 0.394354 5.2:+18173842 None:intergenic
CGACTCCTAAATCTGTTCTA+AGG 0.397762 5.2:-18175594 MS.gene067374:CDS
TCCACCTCAACCTCGGCTTC+GGG 0.399137 5.2:+18176034 None:intergenic
TCAGGGACTAATAACAACTA+TGG 0.437815 5.2:-18174927 MS.gene067374:CDS
ATCTGTAGATGGAGCTGAAA+TGG 0.439210 5.2:-18173868 MS.gene067374:CDS
TGCTCTGTCCATCTTGAGAA+AGG 0.439571 5.2:+18174135 None:intergenic
ATGATGGAAAGTGGAGGGAG+AGG 0.440510 5.2:-18176102 MS.gene067374:CDS
TTCAATTATCAAGAAGTACT+AGG 0.442529 5.2:-18173978 MS.gene067374:CDS
GGAGAGGGTATGATGGAAAG+TGG 0.456884 5.2:-18176111 None:intergenic
GAACTAAAGCCTCAAGTTGA+AGG 0.464648 5.2:-18173939 MS.gene067374:CDS
TGTTATTAGTCCCTGAAGAA+TGG 0.466053 5.2:+18174934 None:intergenic
TAAATTGCAAATGAGAATAG+AGG 0.480939 5.2:-18174204 MS.gene067374:CDS
AACAAAGCGGTCGTGAAGAT+CGG 0.492450 5.2:+18176008 None:intergenic
ATTCACAGAGAAATCCCAAT+AGG 0.498124 5.2:-18174676 MS.gene067374:intron
GTTGACGCTGTCACAAAGCT+TGG 0.498295 5.2:-18175988 MS.gene067374:CDS
ATTTCAGTAACCATTCTTCA+GGG 0.498736 5.2:-18174944 MS.gene067374:CDS
AATGAACTTGTATCTGTAGA+TGG 0.501826 5.2:-18173879 MS.gene067374:CDS
ACTATGGAGGTGATAATGTA+GGG 0.521460 5.2:-18174911 MS.gene067374:CDS
ATGGAAAGTGGAGGGAGAGG+AGG 0.521924 5.2:-18176099 MS.gene067374:CDS
AACTATGGAGGTGATAATGT+AGG 0.537140 5.2:-18174912 MS.gene067374:CDS
GAGGGAGAGGAGGAATTGAA+GGG 0.537312 5.2:-18176089 MS.gene067374:CDS
GGAGGAATTGAAGGGTACGA+TGG 0.538015 5.2:-18176081 MS.gene067374:CDS
ACCCGAAGCCGAGGTTGAGG+TGG 0.538160 5.2:-18176035 MS.gene067374:CDS
GAGACCCGAAGCCGAGGTTG+AGG 0.538216 5.2:-18176038 MS.gene067374:CDS
AACTAAAGCCTCAAGTTGAA+GGG 0.540564 5.2:-18173938 MS.gene067374:CDS
TCAATTATCAAGAAGTACTA+GGG 0.553916 5.2:-18173977 MS.gene067374:CDS
ACTAAAGCCTCAAGTTGAAG+GGG 0.554788 5.2:-18173937 MS.gene067374:CDS
TCCCAATAGGAGAAGCACTA+AGG 0.559258 5.2:-18174663 MS.gene067374:CDS
CAAACAAGCCTTTCTCAAGA+TGG 0.560229 5.2:-18174143 MS.gene067374:CDS
GAGGGTATGATGGAAAGTGG+AGG 0.565667 5.2:-18176108 None:intergenic
AGGGTATGATGGAAAGTGGA+GGG 0.568191 5.2:-18176107 None:intergenic
GGCTCAAGGGAAGTATTTGG+AGG 0.583375 5.2:-18174183 MS.gene067374:CDS
AACTTGCAGAAGTATAGACT+TGG 0.584376 5.2:-18175229 MS.gene067374:intron
GACGCTGTCACAAAGCTTGG+TGG 0.598228 5.2:-18175985 MS.gene067374:CDS
AAATGAGAATAGAGGCTCAA+GGG 0.615268 5.2:-18174196 MS.gene067374:CDS
CAAATGAGAATAGAGGCTCA+AGG 0.629250 5.2:-18174197 MS.gene067374:CDS
TGACGAGAGACCCGAAGCCG+AGG 0.631016 5.2:-18176044 MS.gene067374:CDS
AGAAGAGGTACATACTGTCA+GGG 0.640171 5.2:+18175962 None:intergenic
CTAGACTTACACATTAAAGG+TGG 0.668133 5.2:-18173903 MS.gene067374:CDS
GGGACTAATAACAACTATGG+AGG 0.669862 5.2:-18174924 MS.gene067374:CDS
ATGGAGGTGATAATGTAGGG+GGG 0.671828 5.2:-18174908 MS.gene067374:intron
TATGGAGGTGATAATGTAGG+GGG 0.674191 5.2:-18174909 MS.gene067374:CDS
CTATGGAGGTGATAATGTAG+GGG 0.674421 5.2:-18174910 MS.gene067374:CDS
TCGGTTGTCCACCTCAACCT+CGG 0.676640 5.2:+18176027 None:intergenic
GAGATTGGAAGAACAACTTG+AGG 0.683401 5.2:-18174620 MS.gene067374:intron
TTGTGACAGCGTCAACAAAG+CGG 0.705022 5.2:+18175995 None:intergenic
CTAAAGCCTCAAGTTGAAGG+GGG 0.707595 5.2:-18173936 MS.gene067374:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGATATATTTAATTTATGA+AGG - chr5.2:18174493-18174512 MS.gene067374:intron 10.0%
!!! ATTTTATTTTTTGACTAAAA+AGG + chr5.2:18174431-18174450 None:intergenic 10.0%
!!! TAATTAGCATTTTAATTTTT+AGG - chr5.2:18175193-18175212 MS.gene067374:CDS 10.0%
!!! TCTAAATATTTTCTTATTAA+TGG - chr5.2:18174227-18174246 MS.gene067374:intron 10.0%
!! AAATTAAAATGCTAATTACA+TGG + chr5.2:18175191-18175210 None:intergenic 15.0%
!! AATTAAAATGCTAATTACAT+GGG + chr5.2:18175190-18175209 None:intergenic 15.0%
!! TATTATCATTAATTAGAGAA+TGG - chr5.2:18174579-18174598 MS.gene067374:intron 15.0%
!!! AACACTAGAATTTTAAATAA+AGG + chr5.2:18174099-18174118 None:intergenic 15.0%
!! ATAAACTAGCAGTTAAAAAT+AGG - chr5.2:18174517-18174536 MS.gene067374:intron 20.0%
!! TATATCATCACTATGTAAAA+AGG + chr5.2:18174481-18174500 None:intergenic 20.0%
!!! GCAATTATTTCTATTACATT+TGG - chr5.2:18174922-18174941 MS.gene067374:CDS 20.0%
!!! TTATTTTTTGACTAAAAAGG+TGG + chr5.2:18174428-18174447 None:intergenic 20.0%
!!! TTTTTGTCTTTCATCTTTTT+AGG - chr5.2:18174951-18174970 MS.gene067374:CDS 20.0%
! AATCCAATATGGTTTCATAT+AGG - chr5.2:18176087-18176106 MS.gene067374:CDS 25.0%
! AATTGAAGTTCAAAAGAGAT+TGG - chr5.2:18175297-18175316 MS.gene067374:intron 25.0%
! ATCCAATATGGTTTCATATA+GGG - chr5.2:18176088-18176107 MS.gene067374:CDS 25.0%
! ATGTAAAAAGGCAAAAGAAA+AGG + chr5.2:18174469-18174488 None:intergenic 25.0%
! ATTAACCTTAGAACAGATTT+AGG + chr5.2:18174346-18174365 None:intergenic 25.0%
! TAAATTGCAAATGAGAATAG+AGG - chr5.2:18175728-18175747 MS.gene067374:intron 25.0%
! TAAGGATATATATACTCCTT+TGG + chr5.2:18175655-18175674 None:intergenic 25.0%
! TTCTCAATTGTCAATTACAT+AGG + chr5.2:18175423-18175442 None:intergenic 25.0%
!! ACATTCAAGTTAACTTGAAA+GGG - chr5.2:18174125-18174144 MS.gene067374:CDS 25.0%
!!! AAATCTGTTCTAAGGTTAAT+GGG - chr5.2:18174346-18174365 MS.gene067374:intron 25.0%
!!! AGAAGTTTTAGATGAGAATT+TGG + chr5.2:18175165-18175184 None:intergenic 25.0%
!!! TAAATCTGTTCTAAGGTTAA+TGG - chr5.2:18174345-18174364 MS.gene067374:intron 25.0%
!!! TCAATTATCAAGAAGTACTA+GGG - chr5.2:18175955-18175974 MS.gene067374:intron 25.0%
!!! TTCAATTATCAAGAAGTACT+AGG - chr5.2:18175954-18175973 MS.gene067374:intron 25.0%
!!! TTTTGAAAGAGATGAGAATT+TGG + chr5.2:18175127-18175146 None:intergenic 25.0%
AAAGAGTCTCAAATAAGTGA+AGG - chr5.2:18174794-18174813 MS.gene067374:intron 30.0%
AAGAGTCTCAAATAAGTGAA+GGG - chr5.2:18174795-18174814 MS.gene067374:intron 30.0%
AATGAACTTGTATCTGTAGA+TGG - chr5.2:18176053-18176072 MS.gene067374:CDS 30.0%
AATTTCAGTAACCATTCTTC+AGG - chr5.2:18174987-18175006 MS.gene067374:intron 30.0%
ACAAAACTAGATGAAAGAAG+AGG + chr5.2:18173988-18174007 None:intergenic 30.0%
ATTTCAGTAACCATTCTTCA+GGG - chr5.2:18174988-18175007 MS.gene067374:intron 30.0%
CAATTGGACTGTTTAATTCT+AGG - chr5.2:18175698-18175717 MS.gene067374:intron 30.0%
CTAAGGTTAATGGGATTAAA+AGG - chr5.2:18174355-18174374 MS.gene067374:intron 30.0%
CTAGAATTAAACAGTCCAAT+TGG + chr5.2:18175700-18175719 None:intergenic 30.0%
CTAGTGAATTTCTAACCAAT+TGG - chr5.2:18175682-18175701 MS.gene067374:intron 30.0%
GAATCATAAGCTTTCACAAT+TGG + chr5.2:18174209-18174228 None:intergenic 30.0%
GAATTGTCGTTAAACTAGTT+AGG + chr5.2:18175240-18175259 None:intergenic 30.0%
GGTTAGAAATTCACTAGTTA+GGG + chr5.2:18175679-18175698 None:intergenic 30.0%
GTCTTATAATAAGAGACCAA+AGG - chr5.2:18175636-18175655 MS.gene067374:intron 30.0%
TAATGCATCAAAAGCTCAAT+TGG - chr5.2:18175824-18175843 MS.gene067374:intron 30.0%
TACCCTATATGAAACCATAT+TGG + chr5.2:18176093-18176112 None:intergenic 30.0%
TAGAAGTTGTTACCTGTAAA+TGG + chr5.2:18174405-18174424 None:intergenic 30.0%
TGGTTAGAAATTCACTAGTT+AGG + chr5.2:18175680-18175699 None:intergenic 30.0%
TGGTTAGATTTGACATTGAA+AGG - chr5.2:18174157-18174176 MS.gene067374:CDS 30.0%
! CACATTCAAGTTAACTTGAA+AGG - chr5.2:18174124-18174143 MS.gene067374:CDS 30.0%
! GACTCACATACTAATTTTAG+TGG + chr5.2:18175619-18175638 None:intergenic 30.0%
! GTTTTTCTATGTCTTTGCTA+CGG + chr5.2:18174044-18174063 None:intergenic 30.0%
!! TTGTTTGCTTTTGTGTTTAG+AGG - chr5.2:18174315-18174334 MS.gene067374:intron 30.0%
!!! ACTTGAAAGGGAAGTTTTTT+TGG - chr5.2:18174137-18174156 MS.gene067374:CDS 30.0%
AAATGAGAATAGAGGCTCAA+GGG - chr5.2:18175736-18175755 MS.gene067374:intron 35.0%
AAATTCACTAGTTAGGGCTA+AGG + chr5.2:18175673-18175692 None:intergenic 35.0%
ACTAGGGACAGAAGAAAATA+AGG - chr5.2:18175971-18175990 MS.gene067374:intron 35.0%
AGAGAATGGTGCACTTTAAA+GGG - chr5.2:18174593-18174612 MS.gene067374:intron 35.0%
AGAGTCTCAAATAAGTGAAG+GGG - chr5.2:18174796-18174815 MS.gene067374:intron 35.0%
AGCTGAAATGGAATCCAATA+TGG - chr5.2:18176076-18176095 MS.gene067374:CDS 35.0%
ATTCACAGAGAAATCCCAAT+AGG - chr5.2:18175256-18175275 MS.gene067374:intron 35.0%
CAGCTAGACTTACACATTAA+AGG - chr5.2:18176026-18176045 MS.gene067374:CDS 35.0%
CTAGACTTACACATTAAAGG+TGG - chr5.2:18176029-18176048 MS.gene067374:CDS 35.0%
TAGAGAATGGTGCACTTTAA+AGG - chr5.2:18174592-18174611 MS.gene067374:intron 35.0%
TCAGGGACTAATAACAACTA+TGG - chr5.2:18175005-18175024 MS.gene067374:intron 35.0%
TCATTTGAAGAGCCATTTAC+AGG - chr5.2:18174390-18174409 MS.gene067374:intron 35.0%
TGTTATTAGTCCCTGAAGAA+TGG + chr5.2:18175001-18175020 None:intergenic 35.0%
! AACTATGGAGGTGATAATGT+AGG - chr5.2:18175020-18175039 MS.gene067374:intron 35.0%
! AACTTGCAGAAGTATAGACT+TGG - chr5.2:18174703-18174722 MS.gene067374:intron 35.0%
! ACTATGGAGGTGATAATGTA+GGG - chr5.2:18175021-18175040 MS.gene067374:intron 35.0%
! AGGAAGTATGATGGAGAATT+TGG - chr5.2:18174177-18174196 MS.gene067374:CDS 35.0%
! CTCAGCTTTGTCAAATTTCA+TGG - chr5.2:18175857-18175876 MS.gene067374:intron 35.0%
! GACATTGAAAGGAAGTATGA+TGG - chr5.2:18174168-18174187 MS.gene067374:CDS 35.0%
! GGAAGTATGATGGAGAATTT+GGG - chr5.2:18174178-18174197 MS.gene067374:CDS 35.0%
!! AACTAAAGCCTCAAGTTGAA+GGG - chr5.2:18175994-18176013 MS.gene067374:CDS 35.0%
ATCTGTAGATGGAGCTGAAA+TGG - chr5.2:18176064-18176083 MS.gene067374:CDS 40.0%
CAAACAAGCCTTTCTCAAGA+TGG - chr5.2:18175789-18175808 MS.gene067374:intron 40.0%
CAAATGAGAATAGAGGCTCA+AGG - chr5.2:18175735-18175754 MS.gene067374:intron 40.0%
CGAACTAGAGCGCAATATTT+AGG + chr5.2:18174066-18174085 None:intergenic 40.0%
CGACTCCTAAATCTGTTCTA+AGG - chr5.2:18174338-18174357 MS.gene067374:intron 40.0%
GAGATTGGAAGAACAACTTG+AGG - chr5.2:18175312-18175331 MS.gene067374:intron 40.0%
GAGTCTCAAATAAGTGAAGG+GGG - chr5.2:18174797-18174816 MS.gene067374:intron 40.0%
GGCTTGATTGATGTATGTAG+TGG - chr5.2:18174248-18174267 MS.gene067374:intron 40.0%
GGGACTAATAACAACTATGG+AGG - chr5.2:18175008-18175027 MS.gene067374:intron 40.0%
TGCCTATAATGTATCATGCC+CGG - chr5.2:18175489-18175508 MS.gene067374:intron 40.0%
! AAGAAGAGGTACATACTGTC+AGG + chr5.2:18173974-18173993 None:intergenic 40.0%
! AGAAGAGGTACATACTGTCA+GGG + chr5.2:18173973-18173992 None:intergenic 40.0%
! CTATGGAGGTGATAATGTAG+GGG - chr5.2:18175022-18175041 MS.gene067374:intron 40.0%
! TATGGAGGTGATAATGTAGG+GGG - chr5.2:18175023-18175042 MS.gene067374:intron 40.0%
!! ACTAAAGCCTCAAGTTGAAG+GGG - chr5.2:18175995-18176014 MS.gene067374:CDS 40.0%
!! GAACTAAAGCCTCAAGTTGA+AGG - chr5.2:18175993-18176012 MS.gene067374:CDS 40.0%
AACAAAGCGGTCGTGAAGAT+CGG + chr5.2:18173927-18173946 None:intergenic 45.0%
AGTCCTATTGTAGAGTGCGA+AGG + chr5.2:18175528-18175547 None:intergenic 45.0%
GACCGGGCATGATACATTAT+AGG + chr5.2:18175494-18175513 None:intergenic 45.0%
GCCTTAGTGCTTCTCCTATT+GGG + chr5.2:18175273-18175292 None:intergenic 45.0%
TGCCTTAGTGCTTCTCCTAT+TGG + chr5.2:18175274-18175293 None:intergenic 45.0%
TGCTCTGTCCATCTTGAGAA+AGG + chr5.2:18175800-18175819 None:intergenic 45.0%
TGTCCTTCGCACTCTACAAT+AGG - chr5.2:18175522-18175541 MS.gene067374:intron 45.0%
TTGTGACAGCGTCAACAAAG+CGG + chr5.2:18173940-18173959 None:intergenic 45.0%
! ATGGAGGTGATAATGTAGGG+GGG - chr5.2:18175024-18175043 MS.gene067374:intron 45.0%
!! AGAGGCTCAAGGGAAGTATT+TGG - chr5.2:18175746-18175765 MS.gene067374:intron 45.0%
!! CTAAAGCCTCAAGTTGAAGG+GGG - chr5.2:18175996-18176015 MS.gene067374:CDS 45.0%
!! TCCCAATAGGAGAAGCACTA+AGG - chr5.2:18175269-18175288 MS.gene067374:intron 45.0%
ATTGCACCCCCTTCAACTTG+AGG + chr5.2:18176005-18176024 None:intergenic 50.0%
GAGGGAGAGGAGGAATTGAA+GGG - chr5.2:18173843-18173862 MS.gene067374:CDS 50.0%
GTTGACGCTGTCACAAAGCT+TGG - chr5.2:18173944-18173963 MS.gene067374:CDS 50.0%
! ATGATGGAAAGTGGAGGGAG+AGG - chr5.2:18173830-18173849 MS.gene067374:CDS 50.0%
! GGAGGAATTGAAGGGTACGA+TGG - chr5.2:18173851-18173870 MS.gene067374:CDS 50.0%
!! GGCTCAAGGGAAGTATTTGG+AGG - chr5.2:18175749-18175768 MS.gene067374:intron 50.0%
ATGGAAAGTGGAGGGAGAGG+AGG - chr5.2:18173833-18173852 MS.gene067374:CDS 55.0%
CGAAGGACACAACGAACGAC+CGG + chr5.2:18175511-18175530 None:intergenic 55.0%
GAAGGACACAACGAACGACC+GGG + chr5.2:18175510-18175529 None:intergenic 55.0%
GACGCTGTCACAAAGCTTGG+TGG - chr5.2:18173947-18173966 MS.gene067374:CDS 55.0%
GGAGGGAGAGGAGGAATTGA+AGG - chr5.2:18173842-18173861 MS.gene067374:CDS 55.0%
TCGGTTGTCCACCTCAACCT+CGG + chr5.2:18173908-18173927 None:intergenic 55.0%
GTCCACCTCAACCTCGGCTT+CGG + chr5.2:18173902-18173921 None:intergenic 60.0%
TCCACCTCAACCTCGGCTTC+GGG + chr5.2:18173901-18173920 None:intergenic 60.0%
ACCCGAAGCCGAGGTTGAGG+TGG - chr5.2:18173897-18173916 MS.gene067374:CDS 65.0%
GAGACCCGAAGCCGAGGTTG+AGG - chr5.2:18173894-18173913 MS.gene067374:CDS 65.0%
TGACGAGAGACCCGAAGCCG+AGG - chr5.2:18173888-18173907 MS.gene067374:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 18173830 18176124 18173830 ID=MS.gene067374
chr5.2 mRNA 18173830 18176124 18173830 ID=MS.gene067374.t1;Parent=MS.gene067374
chr5.2 exon 18175977 18176124 18175977 ID=MS.gene067374.t1.exon1;Parent=MS.gene067374.t1
chr5.2 CDS 18175977 18176124 18175977 ID=cds.MS.gene067374.t1;Parent=MS.gene067374.t1
chr5.2 exon 18175543 18175619 18175543 ID=MS.gene067374.t1.exon2;Parent=MS.gene067374.t1
chr5.2 CDS 18175543 18175619 18175543 ID=cds.MS.gene067374.t1;Parent=MS.gene067374.t1
chr5.2 exon 18175182 18175242 18175182 ID=MS.gene067374.t1.exon3;Parent=MS.gene067374.t1
chr5.2 CDS 18175182 18175242 18175182 ID=cds.MS.gene067374.t1;Parent=MS.gene067374.t1
chr5.2 exon 18174909 18174981 18174909 ID=MS.gene067374.t1.exon4;Parent=MS.gene067374.t1
chr5.2 CDS 18174909 18174981 18174909 ID=cds.MS.gene067374.t1;Parent=MS.gene067374.t1
chr5.2 exon 18174621 18174690 18174621 ID=MS.gene067374.t1.exon5;Parent=MS.gene067374.t1
chr5.2 CDS 18174621 18174690 18174621 ID=cds.MS.gene067374.t1;Parent=MS.gene067374.t1
chr5.2 exon 18173830 18174234 18173830 ID=MS.gene067374.t1.exon6;Parent=MS.gene067374.t1
chr5.2 CDS 18173830 18174234 18173830 ID=cds.MS.gene067374.t1;Parent=MS.gene067374.t1
Gene Sequence

>MS.gene067374

ATGATGGAAAGTGGAGGGAGAGGAGGAATTGAAGGGTACGATGGAGTGATGATGACGATGACGAGAGACCCGAAGCCGAGGTTGAGGTGGACAACCGATCTTCACGACCGCTTTGTTGACGCTGTCACAAAGCTTGGTGGCCCTGACAGTATGTACCTCTTCTTTCATCTAGTTTTGTTAAAGTTTTTAAATTATAGTTGTATTTTTGTTGCCGTAGCAAAGACATAGAAAAACCTAAATATTGCGCTCTAGTTCGTAGTTGCAGACCTTTATTTAAAATTCTAGTGTTTGATACACATTCAAGTTAACTTGAAAGGGAAGTTTTTTTGGTTAGATTTGACATTGAAAGGAAGTATGATGGAGAATTTGGGCTAGTCCAATTGTGAAAGCTTATGATTCTAAATATTTTCTTATTAATGGCTTGATTGATGTATGTAGTGGTTCTTAGCTTGTCACAAAATGTTATTAATTTGCAATGTTATTTTTTGTTTGCTTTTGTGTTTAGAGGCGACTCCTAAATCTGTTCTAAGGTTAATGGGATTAAAAGGTTTGACATTATATCATTTGAAGAGCCATTTACAGGTAACAACTTCTACCACCTTTTTAGTCAAAAAATAAAATAATAAAACTTCTACACCTTTTCTTTTGCCTTTTTACATAGTGATGATATATTTAATTTATGAAGGTATAAACTAGCAGTTAAAAATAGGTTGAGAAGTGAAAAGACAAAGTATTAGTTATAGAGATTATATTATCATTAATTAGAGAATGGTGCACTTTAAAGGGTAAACTAATTGAAAAACAAATAGTTGAAGCTTGAGTTTGTGATAAAATATTTACATGACTTTCTATAATGAAGAACATTTTTGTGTTAACTTGCAGAAGTATAGACTTGGACAACATGCTCGAAAACAAAATGAAGAACAATTCAAAGAGAATAACAGTGAGTTCATTACAAATTTTGAAAGAGTCTCAAATAAGTGAAGGGGGTGTTTAAATTGACTTGTTTAAGAGAGTTTACGAATAGAGCTTATGAAAATAACTTATGATATGTTCATAACTTCATAAGCTTTTTTCAGATAACTTTCATTAGCAATTATTTCTATTACATTTGGTTGATTTTTTTGTCTTTCATCTTTTTAGGATGTTCGTATGTAAATTTCAGTAACCATTCTTCAGGGACTAATAACAACTATGGAGGTGATAATGTAGGGGGGTATGTTTAGAATTTAATCAGAATTCACTTATTTTGCTTAATAGTTATTATTATACGTATGACTATATTTTTCATGAGCCAAATTCTCATCTCTTTCAAAAAAAAAAAAAAAAGAGCCAAATTCTCATCTAAAACTTCTTGCCCATGTAATTAGCATTTTAATTTTTAGGTATATACAACTTATTATATTTCCTAACTAGTTTAACGACAATTCACAGAGAAATCCCAATAGGAGAAGCACTAAGGCAACAAATTGAAGTTCAAAAGAGATTGGAAGAACAACTTGAGGTACTCTCTCACTTTTTCTTTTACTTGCACTATAAGACACTTTTTAAAATTTTACATGTATTAATCATTTTTTTTACAAAAATATTCCTATGTAATTGACAATTGAGAAAAGTAAATTCATTATCTTTAATTTTATTTTAAAAACATAAAAGCACTGCCTATAATGTATCATGCCCGGTCGTTCGTTGTGTCCTTCGCACTCTACAATAGGACTCTTCTAGAAAAATAACGCATTTTTTTTCAATAAATTTATTGCTAATATACTATTTTTTTTTCGTAATACCACTAAAATTAGTATGTGAGTCTTATAATAAGAGACCAAAGGAGTATATATATCCTTAGCCCTAACTAGTGAATTTCTAACCAATTGGACTGTTTAATTCTAGGTACAAAATAAATTGCAAATGAGAATAGAGGCTCAAGGGAAGTATTTGGAGGCAGTGTTAGAGAAAGCACAAACAAGCCTTTCTCAAGATGGACAGAGCAATCTTAATGCATCAAAAGCTCAATTGGTTGAGTTCAACTCAGCTTTGTCAAATTTCATGGAAAATATGAATAAAGATAGTAAAGAAAACATACTAGACATGAATGAATTTCATAATAAGAATCATGCTCAAGCTTTCAATTATCAAGAAGTACTAGGGACAGAAGAAAATAAGGAACTAAAGCCTCAAGTTGAAGGGGGTGCAATACAGCTAGACTTACACATTAAAGGTGGCAATGAACTTGTATCTGTAGATGGAGCTGAAATGGAATCCAATATGGTTTCATATAGGGTATACCATTTTTGA
Protein sequence

>MS.gene067374.t1

MMESGGRGGIEGYDGVMMTMTRDPKPRLRWTTDLHDRFVDAVTKLGGPDKATPKSVLRLMGLKGLTLYHLKSHLQKYRLGQHARKQNEEQFKENNRCSYVNFSNHSSGTNNNYGGDNVGGEIPIGEALRQQIEVQKRLEEQLEVQNKLQMRIEAQGKYLEAVLEKAQTSLSQDGQSNLNASKAQLVEFNSALSNFMENMNKDSKENILDMNEFHNKNHAQAFNYQEVLGTEENKELKPQVEGGAIQLDLHIKGGNELVSVDGAEMESNMVSYRVYHF