AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067386

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067386.t1 MTR_5g027550 84.167 240 16 4 1 229 1 229 1.39e-127 360
MS.gene067386.t1 MTR_5g027570 64.463 242 62 7 1 229 1 231 1.14e-98 287
MS.gene067386.t1 MTR_5g088060 52.128 282 73 8 1 229 189 461 3.01e-78 243
MS.gene067386.t1 MTR_5g488160 52.128 282 73 8 1 229 189 461 3.01e-78 243
MS.gene067386.t1 MTR_5g088010 51.779 253 66 6 29 229 1 249 1.83e-75 228
MS.gene067386.t1 MTR_7g067080 56.579 76 30 1 84 159 136 208 1.58e-22 93.6
MS.gene067386.t1 MTR_1g111830 33.146 178 102 6 34 200 67 238 2.29e-22 92.0
MS.gene067386.t1 MTR_3g462790 43.165 139 66 6 29 159 82 215 2.51e-22 93.2
MS.gene067386.t1 MTR_5g069710 57.895 76 29 1 84 159 128 200 1.22e-21 91.3
MS.gene067386.t1 MTR_5g081860 50.538 93 41 2 64 156 105 192 4.91e-21 89.0
MS.gene067386.t1 MTR_2g100930 39.252 107 62 1 84 190 105 208 1.69e-20 88.6
MS.gene067386.t1 MTR_8g063600 50.000 80 39 1 84 163 102 180 1.88e-20 87.8
MS.gene067386.t1 MTR_8g063870 41.463 123 56 4 41 163 60 166 3.95e-20 86.7
MS.gene067386.t1 MTR_4g111975 47.561 82 40 1 84 165 134 212 1.12e-19 85.9
MS.gene067386.t1 MTR_6g092540 52.778 72 31 1 84 155 93 161 2.85e-19 84.3
MS.gene067386.t1 MTR_4g107230 31.737 167 86 5 6 171 22 161 3.40e-19 84.3
MS.gene067386.t1 MTR_4g100630 40.217 92 52 1 76 167 83 171 1.57e-18 82.8
MS.gene067386.t1 MTR_8g101650 52.778 72 30 1 84 155 101 168 3.65e-18 81.3
MS.gene067386.t1 MTR_1g048660 51.389 72 32 1 90 161 14 82 5.64e-18 76.3
MS.gene067386.t1 MTR_4g015130 54.839 62 25 1 98 159 3 61 1.22e-17 75.1
MS.gene067386.t1 MTR_3g104370 45.946 74 40 0 82 155 85 158 1.70e-17 78.6
MS.gene067386.t1 MTR_5g037080 42.609 115 53 4 50 164 71 172 1.89e-17 79.7
MS.gene067386.t1 MTR_5g075760 35.417 144 81 5 37 180 61 192 4.42e-17 77.4
MS.gene067386.t1 MTR_5g075790 32.061 131 80 3 54 180 75 200 1.03e-15 73.6
MS.gene067386.t1 MTR_1g083180 43.590 78 43 1 85 162 58 134 5.86e-15 70.9
MS.gene067386.t1 MTR_1g033620 42.697 89 44 2 104 192 21 102 1.36e-14 70.9
MS.gene067386.t1 MTR_3g070110 48.611 72 33 1 89 156 96 167 1.89e-14 70.1
MS.gene067386.t1 MTR_0036s0260 43.478 69 36 1 88 156 28 93 2.68e-14 69.3
MS.gene067386.t1 MTR_2g090305 44.928 69 35 1 88 156 28 93 2.04e-13 66.6
MS.gene067386.t1 MTR_5g027770 40.566 106 53 3 10 114 95 191 4.74e-11 61.6
MS.gene067386.t1 MTR_8g072990 38.235 68 41 1 83 150 95 161 8.95e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067386.t1 AT5G23650 38.547 179 90 6 37 205 61 229 3.09e-23 96.3
MS.gene067386.t1 AT2G38090 39.726 146 66 6 31 159 69 209 3.39e-23 95.5
MS.gene067386.t1 AT5G08520 37.179 156 81 4 23 165 41 192 5.01e-23 94.7
MS.gene067386.t1 AT5G58900 57.895 76 29 1 84 159 137 209 9.77e-23 94.0
MS.gene067386.t1 AT5G01200 38.514 148 68 5 31 159 71 214 3.11e-22 92.0
MS.gene067386.t1 AT3G11280 39.695 131 69 4 31 159 73 195 8.44e-22 90.9
MS.gene067386.t1 AT3G11280 39.695 131 69 4 31 159 73 195 8.44e-22 90.9
MS.gene067386.t1 AT5G04760 48.913 92 42 2 76 167 87 173 2.78e-21 88.6
MS.gene067386.t1 AT5G05790 38.346 133 74 4 31 159 71 199 9.95e-21 88.2
MS.gene067386.t1 AT5G05790 38.346 133 74 4 31 159 71 199 9.95e-21 88.2
MS.gene067386.t1 AT3G10580 36.364 132 66 4 37 168 56 169 1.23e-20 87.8
MS.gene067386.t1 AT3G10580 36.364 132 66 4 37 168 56 169 2.37e-20 87.4
MS.gene067386.t1 AT1G49010 47.126 87 42 2 80 165 126 209 8.87e-20 86.3
MS.gene067386.t1 AT5G61620 49.383 81 34 2 77 157 101 174 3.93e-19 84.7
MS.gene067386.t1 AT1G19000 41.739 115 58 3 83 197 97 202 6.45e-19 83.6
MS.gene067386.t1 AT1G19000 41.739 115 58 3 83 197 97 202 6.45e-19 83.6
MS.gene067386.t1 AT5G47390 36.066 122 65 3 50 171 65 173 2.64e-18 82.8
MS.gene067386.t1 AT5G56840 48.052 77 38 1 84 160 87 161 7.95e-18 79.7
MS.gene067386.t1 AT1G74840 56.250 64 28 0 71 134 79 142 8.26e-18 79.7
MS.gene067386.t1 AT1G74840 56.250 64 28 0 71 134 79 142 9.61e-18 80.1
MS.gene067386.t1 AT4G09450 36.885 122 60 3 37 158 54 158 2.98e-17 77.4
MS.gene067386.t1 AT3G10590 46.078 102 48 3 62 161 84 180 1.09e-16 75.9
MS.gene067386.t1 AT1G70000 42.553 94 49 2 82 175 92 180 2.33e-16 76.3
MS.gene067386.t1 AT1G70000 42.553 94 49 2 82 175 92 180 2.33e-16 76.3
MS.gene067386.t1 AT3G16350 43.023 86 49 0 50 135 99 184 3.40e-16 77.0

Find 42 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTGTGTCATTGTTGTTTA+AGG 0.102533 5.2:-18325698 None:intergenic
GTCATTGTCCTTGTTATTTA+AGG 0.115096 5.2:-18325773 None:intergenic
TTTCTTCTTCTCTATTTCTT+TGG 0.241888 5.2:-18325650 None:intergenic
TCTCTATTTCTTTGGCAGTT+TGG 0.295652 5.2:-18325642 None:intergenic
TTCTCAAGTTTGTTCATATC+AGG 0.295923 5.2:-18325859 None:intergenic
TTTGCTTCATGACTTCCTTC+TGG 0.307695 5.2:-18323915 None:intergenic
TGTCAAGTTTCTCTTGTTGT+TGG 0.325112 5.2:-18323785 None:intergenic
GCGCAACTTGTTTAACTTGT+TGG 0.342312 5.2:+18325885 MS.gene067386:CDS
TGTTGGTCCATGCTATTCTC+AGG 0.370831 5.2:-18323768 None:intergenic
GATATGTAGGTCGTTTCTAC+AGG 0.371977 5.2:+18325510 MS.gene067386:intron
ATGTTATCATCATTAGAGTT+TGG 0.374150 5.2:-18323843 None:intergenic
AGGCCAACTGATACATTTGT+TGG 0.437602 5.2:-18325810 None:intergenic
CTATTTCTTTGGCAGTTTGG+TGG 0.446999 5.2:-18325639 None:intergenic
TTGGCCTTGCTTTATCCAAT+CGG 0.464369 5.2:+18325826 MS.gene067386:CDS
GACACTGATGTCGTTGAGTC+GGG 0.467326 5.2:+18323804 MS.gene067386:CDS
GGCCAACTGATACATTTGTT+GGG 0.476834 5.2:-18325809 None:intergenic
GCATGACTAGCAATTTGAGT+TGG 0.487768 5.2:-18325598 None:intergenic
GTTGGTCCATGCTATTCTCA+GGG 0.498294 5.2:-18323767 None:intergenic
AAAGAGAGGGCGTAAGGAAA+GGG 0.530997 5.2:+18323935 MS.gene067386:CDS
CAAAGAGAGGGCGTAAGGAA+AGG 0.532803 5.2:+18323934 MS.gene067386:CDS
TCAGGAAGTGCAGTACCGAT+TGG 0.538889 5.2:-18325841 None:intergenic
ATATGTAGGTCGTTTCTACA+GGG 0.546671 5.2:+18325511 MS.gene067386:intron
TGACACTGATGTCGTTGAGT+CGG 0.546703 5.2:+18323803 MS.gene067386:CDS
TTTAAGGTAGCTGTGTCTTG+AGG 0.565081 5.2:-18325757 None:intergenic
TGAAGCAAAGAGAGGGCGTA+AGG 0.567823 5.2:+18323929 MS.gene067386:CDS
CACAGCTACCTTAAATAACA+AGG 0.577999 5.2:+18325765 MS.gene067386:CDS
AACCCAACAAATGTATCAGT+TGG 0.579773 5.2:+18325807 MS.gene067386:CDS
GGCGTAAGGAAAGGGTACAT+TGG 0.585151 5.2:+18323943 MS.gene067386:CDS
AAGGTAGCTGTGTCTTGAGG+AGG 0.587982 5.2:-18325754 None:intergenic
TAAGGAAAGGGTACATTGGA+CGG 0.593107 5.2:+18323947 MS.gene067386:CDS
GAAAGGGTACATTGGACGGA+AGG 0.614122 5.2:+18323951 MS.gene067386:CDS
TTGGTCCATGCTATTCTCAG+GGG 0.618470 5.2:-18323766 None:intergenic
TGGTCCATGCTATTCTCAGG+GGG 0.624422 5.2:-18323765 None:intergenic
ATTAGAGTTTGGAATTGCGA+CGG 0.624770 5.2:-18323832 None:intergenic
ATCATATTGATCTAAATGAG+AGG 0.625540 5.2:+18323727 MS.gene067386:CDS
GAAGTCATGAAGCAAAGAGA+GGG 0.628581 5.2:+18323922 MS.gene067386:CDS
GGAAGTCATGAAGCAAAGAG+AGG 0.629765 5.2:+18323921 MS.gene067386:CDS
CAAGCTGAGTCAGTACCAGA+AGG 0.630435 5.2:+18323900 MS.gene067386:CDS
ATGTAGGTCGTTTCTACAGG+GGG 0.657825 5.2:+18325513 MS.gene067386:intron
TCATTTAGATCAATATGATG+AGG 0.665709 5.2:-18323723 None:intergenic
AGTACCGATTGGATAAAGCA+AGG 0.686649 5.2:-18325830 None:intergenic
TATGTAGGTCGTTTCTACAG+GGG 0.695640 5.2:+18325512 MS.gene067386:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACATTATAATTCTATTTA+GGG + chr5.2:18324011-18324030 MS.gene067386:intron 10.0%
!! TATACATTATAATTCTATTT+AGG + chr5.2:18324010-18324029 MS.gene067386:intron 10.0%
!! TATTTATTATATTCTCTATT+TGG + chr5.2:18324190-18324209 MS.gene067386:intron 10.0%
!!! AAATATATTTTTGTTCATAT+AGG + chr5.2:18325341-18325360 MS.gene067386:intron 10.0%
!!! ATATTGATAATATTAATTAG+TGG + chr5.2:18324634-18324653 MS.gene067386:intron 10.0%
!!! TGAAATTTTATAAAATTTGT+GGG + chr5.2:18324084-18324103 MS.gene067386:intron 10.0%
!!! TTGAAATTTTATAAAATTTG+TGG + chr5.2:18324083-18324102 MS.gene067386:intron 10.0%
!! AAAATGAAGTAACTATATAT+TGG - chr5.2:18325455-18325474 None:intergenic 15.0%
!! ATTATAATGTATATATTCCA+AGG - chr5.2:18324003-18324022 None:intergenic 15.0%
!! TAAATATCTCTATAGATAAA+AGG - chr5.2:18324589-18324608 None:intergenic 15.0%
!!! AATAAATAAGGGTATAATAA+TGG - chr5.2:18325156-18325175 None:intergenic 15.0%
!!! ATAAATAAGGGTATAATAAT+GGG - chr5.2:18325155-18325174 None:intergenic 15.0%
!!! ATTTATATTTTGTGATATGT+AGG + chr5.2:18325497-18325516 MS.gene067386:intron 15.0%
!!! GAAATTTTATAAAATTTGTG+GGG + chr5.2:18324085-18324104 MS.gene067386:intron 15.0%
!! AAAAATAATAAATGCTGCAT+TGG - chr5.2:18325131-18325150 None:intergenic 20.0%
!! AATTTGTAGATAGTGTATTT+AGG + chr5.2:18324729-18324748 MS.gene067386:intron 20.0%
!! GGATGAAATTCAATAAATAA+GGG - chr5.2:18325167-18325186 None:intergenic 20.0%
!! TCTAAATACTACAAAAAATG+TGG - chr5.2:18324878-18324897 None:intergenic 20.0%
!! TCTATAGATAAAAGGTAATT+GGG - chr5.2:18324581-18324600 None:intergenic 20.0%
!! TGGATGAAATTCAATAAATA+AGG - chr5.2:18325168-18325187 None:intergenic 20.0%
!! TGTAGATTATTCCTATAAAA+GGG - chr5.2:18324494-18324513 None:intergenic 20.0%
!! TTGTAGATTATTCCTATAAA+AGG - chr5.2:18324495-18324514 None:intergenic 20.0%
! AAACGACAATAATTTAGAGA+CGG + chr5.2:18325300-18325319 MS.gene067386:intron 25.0%
! ATCATATTGATCTAAATGAG+AGG + chr5.2:18323727-18323746 MS.gene067386:CDS 25.0%
! ATGTTATCATCATTAGAGTT+TGG - chr5.2:18323846-18323865 None:intergenic 25.0%
! CCACTAACTACAATTAATAA+GGG + chr5.2:18325199-18325218 MS.gene067386:intron 25.0%
! CTCTATAGATAAAAGGTAAT+TGG - chr5.2:18324582-18324601 None:intergenic 25.0%
! GCAAAGTAGACTTATAAAAA+GGG + chr5.2:18324771-18324790 MS.gene067386:intron 25.0%
! GCAAATTTAATCTTAGAGTA+AGG + chr5.2:18324316-18324335 MS.gene067386:intron 25.0%
! TCATTTAGATCAATATGATG+AGG - chr5.2:18323726-18323745 None:intergenic 25.0%
! TCCACTAACTACAATTAATA+AGG + chr5.2:18325198-18325217 MS.gene067386:intron 25.0%
! TGCAAAGTAGACTTATAAAA+AGG + chr5.2:18324770-18324789 MS.gene067386:intron 25.0%
!! CTCTAAATTATTGTCGTTTA+AGG - chr5.2:18325299-18325318 None:intergenic 25.0%
!! TTTCTTCTTCTCTATTTCTT+TGG - chr5.2:18325653-18325672 None:intergenic 25.0%
!!! ATTGATTGTGTTGATTTCAA+TGG - chr5.2:18325251-18325270 None:intergenic 25.0%
!!! ATTTCAAGTTGTTTTTCAAG+AGG - chr5.2:18325733-18325752 None:intergenic 25.0%
!!! TTTCAAGTTGTTTTTCAAGA+GGG - chr5.2:18325732-18325751 None:intergenic 25.0%
AAACAAACAGATACAAAGAC+TGG - chr5.2:18324169-18324188 None:intergenic 30.0%
AACAAACAGATACAAAGACT+GGG - chr5.2:18324168-18324187 None:intergenic 30.0%
AAGAGACTATTATTGTGAGA+CGG - chr5.2:18325065-18325084 None:intergenic 30.0%
AGTAGACTTATAAAAAGGGA+CGG + chr5.2:18324775-18324794 MS.gene067386:intron 30.0%
ATTAGCTATGTGATGACAAT+TGG - chr5.2:18324949-18324968 None:intergenic 30.0%
CCCTTATTAATTGTAGTTAG+TGG - chr5.2:18325202-18325221 None:intergenic 30.0%
GGAATAATCTACAAAAATCG+AGG + chr5.2:18324501-18324520 MS.gene067386:intron 30.0%
GTAAGTATTCAATCTATCCT+TGG + chr5.2:18323983-18324002 MS.gene067386:intron 30.0%
TTGAATATCAGTAAAGTCAG+AGG + chr5.2:18325384-18325403 MS.gene067386:intron 30.0%
! TTCTCAAGTTTGTTCATATC+AGG - chr5.2:18325862-18325881 None:intergenic 30.0%
!! GTCATTGTCCTTGTTATTTA+AGG - chr5.2:18325776-18325795 None:intergenic 30.0%
!! TACTGTGTCATTGTTGTTTA+AGG - chr5.2:18325701-18325720 None:intergenic 30.0%
!!! TGAGTATCAATTTTGGAGTA+AGG - chr5.2:18324465-18324484 None:intergenic 30.0%
AAATACTCGTATACCCGTTT+GGG + chr5.2:18324115-18324134 MS.gene067386:intron 35.0%
AACCCAACAAATGTATCAGT+TGG + chr5.2:18325807-18325826 MS.gene067386:CDS 35.0%
ACATGTGTCTAGGTTTAGAT+TGG - chr5.2:18325007-18325026 None:intergenic 35.0%
AGTTAGTGGAGTAGTTAAGT+TGG - chr5.2:18325188-18325207 None:intergenic 35.0%
ATATGTAGGTCGTTTCTACA+GGG + chr5.2:18325511-18325530 MS.gene067386:intron 35.0%
CACAGCTACCTTAAATAACA+AGG + chr5.2:18325765-18325784 MS.gene067386:CDS 35.0%
CTCATCTTCACTCTATACTA+AGG + chr5.2:18324430-18324449 MS.gene067386:intron 35.0%
GACAATAATTTAGAGACGGA+GGG + chr5.2:18325304-18325323 MS.gene067386:intron 35.0%
TAAATACTCGTATACCCGTT+TGG + chr5.2:18324114-18324133 MS.gene067386:intron 35.0%
! ACTTTTCTCCGAATAGAGTA+GGG + chr5.2:18324341-18324360 MS.gene067386:intron 35.0%
! ATTAGAGTTTGGAATTGCGA+CGG - chr5.2:18323835-18323854 None:intergenic 35.0%
! GACAGAGTGAGTATCAATTT+TGG - chr5.2:18324472-18324491 None:intergenic 35.0%
! GCAAGTGTTTGCATTGTTTT+GGG + chr5.2:18325421-18325440 MS.gene067386:intron 35.0%
! TCTCTATTTCTTTGGCAGTT+TGG - chr5.2:18325645-18325664 None:intergenic 35.0%
! TGCAAGTGTTTGCATTGTTT+TGG + chr5.2:18325420-18325439 MS.gene067386:intron 35.0%
! TGTCAAGTTTCTCTTGTTGT+TGG - chr5.2:18323788-18323807 None:intergenic 35.0%
AAACAGATACAAAGACTGGG+AGG - chr5.2:18324165-18324184 None:intergenic 40.0%
AGACTATTATTGTGAGACGG+AGG - chr5.2:18325062-18325081 None:intergenic 40.0%
AGACTTATAAAAAGGGACGG+AGG + chr5.2:18324778-18324797 MS.gene067386:intron 40.0%
AGCTAATGACTAGACACTCA+AGG + chr5.2:18324962-18324981 MS.gene067386:intron 40.0%
AGGCCAACTGATACATTTGT+TGG - chr5.2:18325813-18325832 None:intergenic 40.0%
AGGTTTGTGCACGGTTATTA+AGG - chr5.2:18325279-18325298 None:intergenic 40.0%
CAACTCATCCCTACTCTATT+CGG - chr5.2:18324352-18324371 None:intergenic 40.0%
CCTAGACACATGTAGAACAA+CGG + chr5.2:18325014-18325033 MS.gene067386:intron 40.0%
CGACAATAATTTAGAGACGG+AGG + chr5.2:18325303-18325322 MS.gene067386:intron 40.0%
GAAGTCATGAAGCAAAGAGA+GGG + chr5.2:18323922-18323941 MS.gene067386:CDS 40.0%
GACTATTATTGTGAGACGGA+GGG - chr5.2:18325061-18325080 None:intergenic 40.0%
GATATGTAGGTCGTTTCTAC+AGG + chr5.2:18325510-18325529 MS.gene067386:intron 40.0%
GCATGACTAGCAATTTGAGT+TGG - chr5.2:18325601-18325620 None:intergenic 40.0%
GCTAATGACTAGACACTCAA+GGG + chr5.2:18324963-18324982 MS.gene067386:intron 40.0%
GGCCAACTGATACATTTGTT+GGG - chr5.2:18325812-18325831 None:intergenic 40.0%
TATGTAGGTCGTTTCTACAG+GGG + chr5.2:18325512-18325531 MS.gene067386:intron 40.0%
TTTGCTTCATGACTTCCTTC+TGG - chr5.2:18323918-18323937 None:intergenic 40.0%
! ACTCACTCTGTCCCTTTTAT+AGG + chr5.2:18324480-18324499 MS.gene067386:intron 40.0%
! CTATTTCTTTGGCAGTTTGG+TGG - chr5.2:18325642-18325661 None:intergenic 40.0%
! GACTTTTCTCCGAATAGAGT+AGG + chr5.2:18324340-18324359 MS.gene067386:intron 40.0%
! TAAGGAAAGGGTACATTGGA+CGG + chr5.2:18323947-18323966 MS.gene067386:CDS 40.0%
! TGTCGTTTAAGGTTTGTGCA+CGG - chr5.2:18325288-18325307 None:intergenic 40.0%
! TTTAAGGTAGCTGTGTCTTG+AGG - chr5.2:18325760-18325779 None:intergenic 40.0%
!! AGTACCGATTGGATAAAGCA+AGG - chr5.2:18325833-18325852 None:intergenic 40.0%
!! GCGCAACTTGTTTAACTTGT+TGG + chr5.2:18325885-18325904 MS.gene067386:CDS 40.0%
!! TTGGCCTTGCTTTATCCAAT+CGG + chr5.2:18325826-18325845 MS.gene067386:CDS 40.0%
AAAAACATGGTAGGGGACGT+TGG + chr5.2:18325539-18325558 MS.gene067386:CDS 45.0%
AATGACTAGACACTCAAGGG+TGG + chr5.2:18324966-18324985 MS.gene067386:intron 45.0%
AGGGGGTGAAAAAACATGGT+AGG + chr5.2:18325530-18325549 MS.gene067386:CDS 45.0%
ATGTAGGTCGTTTCTACAGG+GGG + chr5.2:18325513-18325532 MS.gene067386:intron 45.0%
CCGTTGTTCTACATGTGTCT+AGG - chr5.2:18325017-18325036 None:intergenic 45.0%
CTACAGGGGGTGAAAAAACA+TGG + chr5.2:18325526-18325545 MS.gene067386:CDS 45.0%
GGAAGTCATGAAGCAAAGAG+AGG + chr5.2:18323921-18323940 MS.gene067386:CDS 45.0%
GGGGGTGAAAAAACATGGTA+GGG + chr5.2:18325531-18325550 MS.gene067386:CDS 45.0%
GGGGTGAAAAAACATGGTAG+GGG + chr5.2:18325532-18325551 MS.gene067386:CDS 45.0%
GTTGGTCCATGCTATTCTCA+GGG - chr5.2:18323770-18323789 None:intergenic 45.0%
TACAAAGACTGGGAGGACAT+TGG - chr5.2:18324158-18324177 None:intergenic 45.0%
TGACACTGATGTCGTTGAGT+CGG + chr5.2:18323803-18323822 MS.gene067386:CDS 45.0%
TGACCCAGGATTAACCCAAA+CGG - chr5.2:18324132-18324151 None:intergenic 45.0%
TGTTGGTCCATGCTATTCTC+AGG - chr5.2:18323771-18323790 None:intergenic 45.0%
TTGGTCCATGCTATTCTCAG+GGG - chr5.2:18323769-18323788 None:intergenic 45.0%
! AAAGAGAGGGCGTAAGGAAA+GGG + chr5.2:18323935-18323954 MS.gene067386:CDS 45.0%
! ATACCCGTTTGGGTTAATCC+TGG + chr5.2:18324125-18324144 MS.gene067386:intron 45.0%
! TACCCGTTTGGGTTAATCCT+GGG + chr5.2:18324126-18324145 MS.gene067386:intron 45.0%
AAGGTAGCTGTGTCTTGAGG+AGG - chr5.2:18325757-18325776 None:intergenic 50.0%
AGATCCCCCTGAGAATAGCA+TGG + chr5.2:18323761-18323780 MS.gene067386:CDS 50.0%
CAAGCTGAGTCAGTACCAGA+AGG + chr5.2:18323900-18323919 MS.gene067386:CDS 50.0%
GACACTGATGTCGTTGAGTC+GGG + chr5.2:18323804-18323823 MS.gene067386:CDS 50.0%
GACCCAGGATTAACCCAAAC+GGG - chr5.2:18324131-18324150 None:intergenic 50.0%
TGGTCCATGCTATTCTCAGG+GGG - chr5.2:18323768-18323787 None:intergenic 50.0%
! CAAAGAGAGGGCGTAAGGAA+AGG + chr5.2:18323934-18323953 MS.gene067386:CDS 50.0%
! GAAAGGGTACATTGGACGGA+AGG + chr5.2:18323951-18323970 MS.gene067386:CDS 50.0%
! GGCGTAAGGAAAGGGTACAT+TGG + chr5.2:18323943-18323962 MS.gene067386:CDS 50.0%
! GTTTGGGTTAATCCTGGGTC+AGG + chr5.2:18324131-18324150 MS.gene067386:intron 50.0%
! TGAAGCAAAGAGAGGGCGTA+AGG + chr5.2:18323929-18323948 MS.gene067386:CDS 50.0%
!! TCAGGAAGTGCAGTACCGAT+TGG - chr5.2:18325844-18325863 None:intergenic 50.0%
GACACTCAAGGGTGGAGAAC+TGG + chr5.2:18324974-18324993 MS.gene067386:intron 55.0%
GAGGACATTGGACCTGACCC+AGG - chr5.2:18324146-18324165 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 18323696 18325921 18323696 ID=MS.gene067386
chr5.2 mRNA 18323696 18325921 18323696 ID=MS.gene067386.t1;Parent=MS.gene067386
chr5.2 exon 18323696 18323982 18323696 ID=MS.gene067386.t1.exon1;Parent=MS.gene067386.t1
chr5.2 CDS 18323696 18323982 18323696 ID=cds.MS.gene067386.t1;Parent=MS.gene067386.t1
chr5.2 exon 18325519 18325921 18325519 ID=MS.gene067386.t1.exon2;Parent=MS.gene067386.t1
chr5.2 CDS 18325519 18325921 18325519 ID=cds.MS.gene067386.t1;Parent=MS.gene067386.t1
Gene Sequence

>MS.gene067386

ATGGAAGGAAGAAAAGTTCAACAATTACCTCATCATATTGATCTAAATGAGAGGACTTCAATTCAAGATCCCCCTGAGAATAGCATGGACCAACAACAAGAGAAACTTGACACTGATGTCGTTGAGTCGGGTTGTGCCGTCGCAATTCCAAACTCTAATGATGATAACATTGCGACTCTAAACTCTAATGATAATAACTTAGTTCAAGCTGAGTCAGTACCAGAAGGAAGTCATGAAGCAAAGAGAGGGCGTAAGGAAAGGGTACATTGGACGGAAGGTGAACATAAGTAAGTATTCAATCTATCCTTGGAATATATACATTATAATTCTATTTAGGGTTAAATATATTTGATCAATTGAAGTTATTAGATATGTATTAAATAGTTTTTGAAATTTTATAAAATTTGTGGGGAGTTGATAAATACTCGTATACCCGTTTGGGTTAATCCTGGGTCAGGTCCAATGTCCTCCCAGTCTTTGTATCTGTTTGTTTTTATTTATTATATTCTCTATTTGGCTTAAAAAAAATATATATACTTAATACTCAACAGTGATGAATGAATCATATGTTTACAATGAATTTTTCTTTTTTGAAATGTATCTTTACGATGTAACTCTATGCAAATTTAATCTTAGAGTAAGGTGACTTTTCTCCGAATAGAGTAGGGATGAGTTGACTCAGAAGTAACTCTAAGATAACTTCTCTTTATATTTTACTAAGCACAATAACTCAACTCATCTTCACTCTATACTAAGGAGATACTCACCTTACTCCAAAATTGATACTCACTCTGTCCCTTTTATAGGAATAATCTACAAAAATCGAGGTATTAAGAAAAATGTTTGAAGTAGTAAATATTTTATAAATGTATGTTACTATTACCCAATTACCTTTTATCTATAGAGATATTTAATGTTAACTTTTCAAATTTCAATGTATATTGATAATATTAATTAGTGGTGTATTTAAAAAAAAAGTAATAAATGCATCTAAAAATTTGTTTTAAAAAAAAAAGTAATAAATGCATCTAAAAATTTGTAGATAGTGTATTTAGGAACAATTTTTTTTTTTTTTGCAAAGTAGACTTATAAAAAGGGACGGAGGAGTGAATCATTATAAATATTGAATTCATAAATTCAACATCACATCAAAAGTTCTTATTAAATACTATATCAACTCCACATTTTTTGTAGTATTTAGATTTTAGAAACTGTTTAATATTTCATCAAATAATTTCGATGAGTAAATACCAATTGTCATCACATAGCTAATGACTAGACACTCAAGGGTGGAGAACTGGAAAATCTCCAATCTAAACCTAGACACATGTAGAACAACGGATAAAAAATCACACATATACTCCCTCCGTCTCACAATAATAGTCTCTTTAACATTTTTTCTTTTTCTCAAAATAATTGTCACTTTAGAATACCAATGCAGCATTTATTATTTTTTCCCATTATTATACCCTTATTTATTGAATTTCATCCAACTTAACTACTCCACTAACTACAATTAATAAGGGTATTTTAGTAAATGATATTAATTTTACCATTGAAATCAACACAATCAATCATTTCCTTAATAACCGTGCACAAACCTTAAACGACAATAATTTAGAGACGGAGGGAGTAGCTTTTTAGAAAATATATTTTTGTTCATATAGGTGCATATTAATATTTCTATGTTGAATATCAGTAAAGTCAGAGGAAAAAAAAATCAATGCAAGTGTTTGCATTGTTTTGGGTACATATACCAATATATAGTTACTTCATTTTTCTTCTCTTGTTATTTAATTGAATTTATATTTTGTGATATGTAGGTCGTTTCTACAGGGGGTGAAAAAACATGGTAGGGGACGTTGGAAAGATATTTCAAAAGAATTTGTGCGAACAAAAACTCCAACTCAAATTGCTAGTCATGCTCAAAAATACTTTGTTCACCACCAAACTGCCAAAGAAATAGAGAAGAAGAAAAAGAGAAGAAGTATTCACGACATAACCTTAAACAACAATGACACAGTAGTCACTCTCCCTCTTGAAAAACAACTTGAAATTCCTCCTCAAGACACAGCTACCTTAAATAACAAGGACAATGACAACTTGCAACAAACCCAACAAATGTATCAGTTGGCCTTGCTTTATCCAATCGGTACTGCACTTCCTGATATGAACAAACTTGAGAAGATGCGCAACTTGTTTAACTTGTTGGCAAAAGACCTTTGA
Protein sequence

>MS.gene067386.t1

MEGRKVQQLPHHIDLNERTSIQDPPENSMDQQQEKLDTDVVESGCAVAIPNSNDDNIATLNSNDNNLVQAESVPEGSHEAKRGRKERVHWTEGEHKSFLQGVKKHGRGRWKDISKEFVRTKTPTQIASHAQKYFVHHQTAKEIEKKKKRRSIHDITLNNNDTVVTLPLEKQLEIPPQDTATLNNKDNDNLQQTQQMYQLALLYPIGTALPDMNKLEKMRNLFNLLAKDL