Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067586.t1 | AES90030.2 | 87.2 | 156 | 17 | 1 | 10 | 162 | 38 | 193 | 1.10E-69 | 272.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067586.t1 | Q7XLP4 | 38.2 | 110 | 64 | 2 | 34 | 140 | 83 | 191 | 6.0e-16 | 85.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067586.t1 | G7JHL3 | 87.2 | 156 | 17 | 1 | 10 | 162 | 38 | 193 | 7.9e-70 | 272.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene067586.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067586.t1 | MTR_4g083390 | 87.179 | 156 | 17 | 1 | 10 | 162 | 38 | 193 | 2.10e-96 | 276 |
| MS.gene067586.t1 | MTR_4g083390 | 91.729 | 133 | 11 | 0 | 30 | 162 | 1 | 133 | 3.41e-89 | 256 |
| MS.gene067586.t1 | MTR_4g083390 | 86.260 | 131 | 18 | 0 | 30 | 160 | 1 | 131 | 2.96e-81 | 237 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGGAATTGAACCGCTTTC+AGG | 0.230161 | 4.1:+24387804 | MS.gene067586:CDS |
| TTCAAAGTTCAAATTCTTAG+AGG | 0.303256 | 4.1:+24388209 | MS.gene067586:CDS |
| AGTTGGGCCACGACTTATAA+TGG | 0.308961 | 4.1:+24388065 | MS.gene067586:CDS |
| ACGGGTACTAAGATGTGTTT+TGG | 0.320299 | 4.1:-24387853 | None:intergenic |
| TTCCGACTATTCTCAAATAT+CGG | 0.320685 | 4.1:+24388036 | MS.gene067586:CDS |
| GCATCCCTCTCACCGGTTCC+AGG | 0.356774 | 4.1:-24388263 | None:intergenic |
| GAGAATAGTCGGAACCGATT+TGG | 0.387243 | 4.1:-24388027 | None:intergenic |
| ACACATCTTAGTACCCGTTA+TGG | 0.396282 | 4.1:+24387858 | MS.gene067586:CDS |
| GAAGATAATTATTTGAAGCT+TGG | 0.405476 | 4.1:+24388137 | MS.gene067586:CDS |
| TCCGACTATTCTCAAATATC+GGG | 0.408506 | 4.1:+24388037 | MS.gene067586:CDS |
| GCCAACGCTATCAAACAGTT+TGG | 0.414501 | 4.1:-24388099 | None:intergenic |
| AACTGTTTGATAGCGTTGGC+TGG | 0.415821 | 4.1:+24388102 | MS.gene067586:CDS |
| GAAAGGACCACATGAAAGTA+TGG | 0.437117 | 4.1:-24387831 | None:intergenic |
| CTTTACGAATTTCCTGGAAC+CGG | 0.450094 | 4.1:+24388251 | MS.gene067586:CDS |
| TGAAATTACCAATCCATAAC+GGG | 0.462093 | 4.1:-24387871 | None:intergenic |
| CAAATATCGGGGTAAGAGTT+GGG | 0.464634 | 4.1:+24388049 | MS.gene067586:CDS |
| GATTACTAATCTATGACAAA+TGG | 0.467072 | 4.1:-24388287 | None:intergenic |
| TCAAATATCGGGGTAAGAGT+TGG | 0.468409 | 4.1:+24388048 | MS.gene067586:CDS |
| TGAAGAACTTCCTTCCAAAT+CGG | 0.469161 | 4.1:+24388013 | MS.gene067586:CDS |
| GTTGGGCCACGACTTATAAT+GGG | 0.482324 | 4.1:+24388066 | MS.gene067586:CDS |
| ATGTCGTCTTCAAAATCAGC+TGG | 0.502794 | 4.1:+24387619 | MS.gene067586:CDS |
| TCTTAGTACCCGTTATGGAT+TGG | 0.505736 | 4.1:+24387863 | MS.gene067586:CDS |
| ACCAAACTGTTTGATAGCGT+TGG | 0.508522 | 4.1:+24388098 | MS.gene067586:CDS |
| ATGAAATTACCAATCCATAA+CGG | 0.511215 | 4.1:-24387872 | None:intergenic |
| CCCCGATATTTGAGAATAGT+CGG | 0.515602 | 4.1:-24388038 | None:intergenic |
| ACCTTCGACAAATATGCAAA+CGG | 0.530039 | 4.1:+24387782 | MS.gene067586:CDS |
| ACTGTTTGATAGCGTTGGCT+GGG | 0.539366 | 4.1:+24388103 | MS.gene067586:CDS |
| GAAGGTGATGATAACGAGGC+TGG | 0.539673 | 4.1:-24387765 | None:intergenic |
| TCCGTTTGCATATTTGTCGA+AGG | 0.549182 | 4.1:-24387783 | None:intergenic |
| GTTCTGCCCATTATAAGTCG+TGG | 0.557426 | 4.1:-24388072 | None:intergenic |
| TCTTCAAAATCAGCTGGAAG+AGG | 0.564849 | 4.1:+24387625 | MS.gene067586:CDS |
| AAATGGTGCATCCCTCTCAC+CGG | 0.578086 | 4.1:-24388270 | None:intergenic |
| AGGAGTGCCATACTTTCATG+TGG | 0.578309 | 4.1:+24387824 | MS.gene067586:CDS |
| TCGACAAATATGCAAACGGA+TGG | 0.583245 | 4.1:+24387786 | MS.gene067586:CDS |
| GAGTAGCAGTCAAGTTGCTG+TGG | 0.583739 | 4.1:+24387737 | MS.gene067586:CDS |
| TCTTCACATATGCTGCTGCT+CGG | 0.585483 | 4.1:-24387996 | None:intergenic |
| ATTTCCTGGAACCGGTGAGA+GGG | 0.587927 | 4.1:+24388259 | MS.gene067586:CDS |
| AGTAGCAGTCAAGTTGCTGT+GGG | 0.603556 | 4.1:+24387738 | MS.gene067586:CDS |
| AATTTCCTGGAACCGGTGAG+AGG | 0.633829 | 4.1:+24388258 | MS.gene067586:CDS |
| ATAGTCGGAACCGATTTGGA+AGG | 0.643344 | 4.1:-24388023 | None:intergenic |
| AAGTATGGCACTCCTGAAAG+CGG | 0.678549 | 4.1:-24387816 | None:intergenic |
| CCGACTATTCTCAAATATCG+GGG | 0.681942 | 4.1:+24388038 | MS.gene067586:CDS |
| TGTCGAAGGTGATGATAACG+AGG | 0.728967 | 4.1:-24387769 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATCTTGTATTGTATTGT+TGG | + | chr4.1:24387963-24387982 | MS.gene067586:intron | 20.0% |
| !! | TAATGTGATATGAAATACTA+AGG | - | chr4.1:24387658-24387677 | None:intergenic | 20.0% |
| !! | TTATAGATCAAACTAAATGA+AGG | + | chr4.1:24387912-24387931 | MS.gene067586:intron | 20.0% |
| ! | AAGATTAACTTTCATGTACA+CGG | + | chr4.1:24387680-24387699 | MS.gene067586:intron | 25.0% |
| ! | ATGAAATTACCAATCCATAA+CGG | - | chr4.1:24387875-24387894 | None:intergenic | 25.0% |
| ! | TATTGTATTGTTGGCATATA+GGG | + | chr4.1:24387972-24387991 | MS.gene067586:intron | 25.0% |
| ! | TTCAAAGTTCAAATTCTTAG+AGG | + | chr4.1:24388209-24388228 | MS.gene067586:CDS | 25.0% |
| !! | ATCAAACTAAATGAAGGTTT+TGG | + | chr4.1:24387918-24387937 | MS.gene067586:intron | 25.0% |
| !! | GAAGATAATTATTTGAAGCT+TGG | + | chr4.1:24388137-24388156 | MS.gene067586:CDS | 25.0% |
| ATTGTATTGTTGGCATATAG+GGG | + | chr4.1:24387973-24387992 | MS.gene067586:intron | 30.0% | |
| GGAAATTCGTAAAGCAAAAA+TGG | - | chr4.1:24388245-24388264 | None:intergenic | 30.0% | |
| GTATTGTATTGTTGGCATAT+AGG | + | chr4.1:24387971-24387990 | MS.gene067586:intron | 30.0% | |
| GTGTACATGAAAGTTAATCT+TGG | - | chr4.1:24387681-24387700 | None:intergenic | 30.0% | |
| TGAAATTACCAATCCATAAC+GGG | - | chr4.1:24387874-24387893 | None:intergenic | 30.0% | |
| TTCCGACTATTCTCAAATAT+CGG | + | chr4.1:24388036-24388055 | MS.gene067586:CDS | 30.0% | |
| ! | TTTTTGCTTTACGAATTTCC+TGG | + | chr4.1:24388245-24388264 | MS.gene067586:CDS | 30.0% |
| !!! | ACTAAATGAAGGTTTTGGAA+AGG | + | chr4.1:24387923-24387942 | MS.gene067586:intron | 30.0% |
| !!! | TACTAAGATGTGTTTTGGAA+AGG | - | chr4.1:24387851-24387870 | None:intergenic | 30.0% |
| ACCTTCGACAAATATGCAAA+CGG | + | chr4.1:24387782-24387801 | MS.gene067586:CDS | 35.0% | |
| TCCGACTATTCTCAAATATC+GGG | + | chr4.1:24388037-24388056 | MS.gene067586:CDS | 35.0% | |
| TGAAGAACTTCCTTCCAAAT+CGG | + | chr4.1:24388013-24388032 | MS.gene067586:CDS | 35.0% | |
| TTAACTTTCATGTACACGGT+CGG | + | chr4.1:24387684-24387703 | MS.gene067586:intron | 35.0% | |
| ACACATCTTAGTACCCGTTA+TGG | + | chr4.1:24387858-24387877 | MS.gene067586:CDS | 40.0% | |
| ATGTCGTCTTCAAAATCAGC+TGG | + | chr4.1:24387619-24387638 | MS.gene067586:CDS | 40.0% | |
| CAAATATCGGGGTAAGAGTT+GGG | + | chr4.1:24388049-24388068 | MS.gene067586:CDS | 40.0% | |
| CCCCGATATTTGAGAATAGT+CGG | - | chr4.1:24388041-24388060 | None:intergenic | 40.0% | |
| CCGACTATTCTCAAATATCG+GGG | + | chr4.1:24388038-24388057 | MS.gene067586:CDS | 40.0% | |
| CTTTACGAATTTCCTGGAAC+CGG | + | chr4.1:24388251-24388270 | MS.gene067586:CDS | 40.0% | |
| GAAAGGACCACATGAAAGTA+TGG | - | chr4.1:24387834-24387853 | None:intergenic | 40.0% | |
| GCTCATGAAAAACAGTGAAG+AGG | + | chr4.1:24388178-24388197 | MS.gene067586:CDS | 40.0% | |
| TCAAATATCGGGGTAAGAGT+TGG | + | chr4.1:24388048-24388067 | MS.gene067586:CDS | 40.0% | |
| TCCGTTTGCATATTTGTCGA+AGG | - | chr4.1:24387786-24387805 | None:intergenic | 40.0% | |
| TCGACAAATATGCAAACGGA+TGG | + | chr4.1:24387786-24387805 | MS.gene067586:CDS | 40.0% | |
| TCTTAGTACCCGTTATGGAT+TGG | + | chr4.1:24387863-24387882 | MS.gene067586:CDS | 40.0% | |
| TCTTCAAAATCAGCTGGAAG+AGG | + | chr4.1:24387625-24387644 | MS.gene067586:CDS | 40.0% | |
| ! | ACGGGTACTAAGATGTGTTT+TGG | - | chr4.1:24387856-24387875 | None:intergenic | 40.0% |
| !! | ACCAAACTGTTTGATAGCGT+TGG | + | chr4.1:24388098-24388117 | MS.gene067586:CDS | 40.0% |
| AAGTATGGCACTCCTGAAAG+CGG | - | chr4.1:24387819-24387838 | None:intergenic | 45.0% | |
| AGTTGGGCCACGACTTATAA+TGG | + | chr4.1:24388065-24388084 | MS.gene067586:CDS | 45.0% | |
| GAGAATAGTCGGAACCGATT+TGG | - | chr4.1:24388030-24388049 | None:intergenic | 45.0% | |
| GATGGAATTGAACCGCTTTC+AGG | + | chr4.1:24387804-24387823 | MS.gene067586:CDS | 45.0% | |
| GCCAACGCTATCAAACAGTT+TGG | - | chr4.1:24388102-24388121 | None:intergenic | 45.0% | |
| GTTCTGCCCATTATAAGTCG+TGG | - | chr4.1:24388075-24388094 | None:intergenic | 45.0% | |
| GTTGGGCCACGACTTATAAT+GGG | + | chr4.1:24388066-24388085 | MS.gene067586:CDS | 45.0% | |
| TCTTCACATATGCTGCTGCT+CGG | - | chr4.1:24387999-24388018 | None:intergenic | 45.0% | |
| ! | AGTAGCAGTCAAGTTGCTGT+GGG | + | chr4.1:24387738-24387757 | MS.gene067586:CDS | 45.0% |
| ! | TGTCGAAGGTGATGATAACG+AGG | - | chr4.1:24387772-24387791 | None:intergenic | 45.0% |
| !! | AACTGTTTGATAGCGTTGGC+TGG | + | chr4.1:24388102-24388121 | MS.gene067586:CDS | 45.0% |
| !! | ACTGTTTGATAGCGTTGGCT+GGG | + | chr4.1:24388103-24388122 | MS.gene067586:CDS | 45.0% |
| !! | AGGAGTGCCATACTTTCATG+TGG | + | chr4.1:24387824-24387843 | MS.gene067586:CDS | 45.0% |
| !! | ATAGTCGGAACCGATTTGGA+AGG | - | chr4.1:24388026-24388045 | None:intergenic | 45.0% |
| AAATGGTGCATCCCTCTCAC+CGG | - | chr4.1:24388273-24388292 | None:intergenic | 50.0% | |
| AATTTCCTGGAACCGGTGAG+AGG | + | chr4.1:24388258-24388277 | MS.gene067586:CDS | 50.0% | |
| ATTTCCTGGAACCGGTGAGA+GGG | + | chr4.1:24388259-24388278 | MS.gene067586:CDS | 50.0% | |
| ! | GAAGGTGATGATAACGAGGC+TGG | - | chr4.1:24387768-24387787 | None:intergenic | 50.0% |
| ! | GAGTAGCAGTCAAGTTGCTG+TGG | + | chr4.1:24387737-24387756 | MS.gene067586:CDS | 50.0% |
| !! | GCATCCCTCTCACCGGTTCC+AGG | - | chr4.1:24388266-24388285 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 24387619 | 24388304 | 24387619 | ID=MS.gene067586 |
| chr4.1 | mRNA | 24387619 | 24388304 | 24387619 | ID=MS.gene067586.t1;Parent=MS.gene067586 |
| chr4.1 | exon | 24387619 | 24387646 | 24387619 | ID=MS.gene067586.t1.exon1;Parent=MS.gene067586.t1 |
| chr4.1 | CDS | 24387619 | 24387646 | 24387619 | ID=cds.MS.gene067586.t1;Parent=MS.gene067586.t1 |
| chr4.1 | exon | 24387736 | 24387884 | 24387736 | ID=MS.gene067586.t1.exon2;Parent=MS.gene067586.t1 |
| chr4.1 | CDS | 24387736 | 24387884 | 24387736 | ID=cds.MS.gene067586.t1;Parent=MS.gene067586.t1 |
| chr4.1 | exon | 24387993 | 24388304 | 24387993 | ID=MS.gene067586.t1.exon3;Parent=MS.gene067586.t1 |
| chr4.1 | CDS | 24387993 | 24388304 | 24387993 | ID=cds.MS.gene067586.t1;Parent=MS.gene067586.t1 |
| Gene Sequence |
| Protein sequence |
