Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067641.t1 | XP_003605525.1 | 77.8 | 99 | 22 | 0 | 1 | 99 | 1 | 99 | 7.20E-35 | 156.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067641.t1 | Q9FJK3 | 56.0 | 100 | 44 | 0 | 1 | 100 | 1 | 100 | 1.2e-27 | 123.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067641.t1 | G7JN85 | 77.8 | 99 | 22 | 0 | 1 | 99 | 1 | 99 | 5.2e-35 | 156.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene067641.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067641.t1 | MTR_4g032620 | 77.778 | 99 | 22 | 0 | 1 | 99 | 1 | 99 | 2.16e-51 | 161 |
| MS.gene067641.t1 | MTR_8g036130 | 62.376 | 101 | 36 | 1 | 1 | 99 | 1 | 101 | 3.02e-41 | 136 |
| MS.gene067641.t1 | MTR_2g035610 | 62.626 | 99 | 37 | 0 | 1 | 99 | 1 | 99 | 2.08e-38 | 125 |
| MS.gene067641.t1 | MTR_3g065100 | 60.606 | 99 | 39 | 0 | 1 | 99 | 1 | 99 | 7.75e-37 | 124 |
| MS.gene067641.t1 | MTR_4g031910 | 62.626 | 99 | 37 | 0 | 1 | 99 | 1 | 99 | 1.08e-36 | 124 |
| MS.gene067641.t1 | MTR_4g032260 | 65.657 | 99 | 34 | 0 | 1 | 99 | 1 | 99 | 1.79e-36 | 121 |
| MS.gene067641.t1 | MTR_4g028800 | 75.342 | 73 | 16 | 1 | 1 | 73 | 1 | 71 | 3.66e-36 | 117 |
| MS.gene067641.t1 | MTR_3g466980 | 50.505 | 99 | 49 | 0 | 1 | 99 | 1 | 99 | 3.62e-35 | 120 |
| MS.gene067641.t1 | MTR_3g466830 | 48.958 | 96 | 49 | 0 | 4 | 99 | 3 | 98 | 9.82e-34 | 114 |
| MS.gene067641.t1 | MTR_4g032290 | 60.606 | 99 | 39 | 0 | 1 | 99 | 1 | 99 | 1.19e-32 | 114 |
| MS.gene067641.t1 | MTR_4g063790 | 47.917 | 96 | 50 | 0 | 1 | 96 | 1 | 96 | 2.59e-31 | 108 |
| MS.gene067641.t1 | MTR_5g075380 | 46.465 | 99 | 53 | 0 | 1 | 99 | 1 | 99 | 4.42e-30 | 105 |
| MS.gene067641.t1 | MTR_3g466890 | 45.263 | 95 | 52 | 0 | 5 | 99 | 4 | 98 | 7.82e-30 | 104 |
| MS.gene067641.t1 | MTR_2g016210 | 45.918 | 98 | 53 | 0 | 1 | 98 | 1 | 98 | 1.90e-29 | 105 |
| MS.gene067641.t1 | MTR_3g467080 | 42.268 | 97 | 56 | 0 | 1 | 97 | 1 | 97 | 2.77e-28 | 100 |
| MS.gene067641.t1 | MTR_5g047580 | 42.424 | 99 | 56 | 1 | 1 | 99 | 1 | 98 | 1.85e-27 | 98.6 |
| MS.gene067641.t1 | MTR_3g031240 | 39.394 | 99 | 60 | 0 | 1 | 99 | 1 | 99 | 1.95e-27 | 99.0 |
| MS.gene067641.t1 | MTR_1g077390 | 43.299 | 97 | 54 | 1 | 1 | 97 | 1 | 96 | 8.89e-27 | 96.7 |
| MS.gene067641.t1 | MTR_1g090783 | 42.708 | 96 | 54 | 1 | 4 | 99 | 3 | 97 | 6.39e-26 | 94.4 |
| MS.gene067641.t1 | MTR_1g090710 | 40.404 | 99 | 58 | 1 | 1 | 99 | 1 | 98 | 8.33e-26 | 94.4 |
| MS.gene067641.t1 | MTR_1g090697 | 42.424 | 99 | 56 | 1 | 1 | 99 | 1 | 98 | 2.83e-25 | 92.8 |
| MS.gene067641.t1 | MTR_3g031100 | 41.414 | 99 | 58 | 0 | 1 | 99 | 1 | 99 | 5.87e-25 | 94.4 |
| MS.gene067641.t1 | MTR_7g106510 | 42.105 | 95 | 55 | 0 | 4 | 98 | 7 | 101 | 7.59e-23 | 91.7 |
| MS.gene067641.t1 | MTR_4g019670 | 40.000 | 95 | 57 | 0 | 4 | 98 | 7 | 101 | 4.62e-22 | 89.4 |
| MS.gene067641.t1 | MTR_2g035580 | 33.684 | 95 | 63 | 0 | 1 | 95 | 1 | 95 | 8.82e-22 | 84.0 |
| MS.gene067641.t1 | MTR_7g055800 | 46.316 | 95 | 23 | 2 | 1 | 95 | 1 | 67 | 1.26e-21 | 81.6 |
| MS.gene067641.t1 | MTR_1g084950 | 43.299 | 97 | 54 | 1 | 1 | 97 | 1 | 96 | 2.25e-21 | 82.8 |
| MS.gene067641.t1 | MTR_7g055940 | 45.263 | 95 | 24 | 2 | 1 | 95 | 1 | 67 | 7.09e-21 | 79.3 |
| MS.gene067641.t1 | MTR_7g011950 | 39.394 | 99 | 59 | 1 | 1 | 99 | 1 | 98 | 1.07e-20 | 81.3 |
| MS.gene067641.t1 | MTR_3g465410 | 46.377 | 69 | 37 | 0 | 29 | 97 | 1 | 69 | 2.74e-20 | 79.7 |
| MS.gene067641.t1 | MTR_1g077320 | 38.384 | 99 | 60 | 1 | 1 | 99 | 1 | 98 | 1.92e-19 | 78.2 |
| MS.gene067641.t1 | MTR_3g093900 | 37.778 | 90 | 56 | 0 | 4 | 93 | 7 | 96 | 8.42e-19 | 80.1 |
| MS.gene067641.t1 | MTR_1g077300 | 42.268 | 97 | 55 | 1 | 1 | 97 | 1 | 96 | 9.22e-19 | 76.3 |
| MS.gene067641.t1 | MTR_4g028720 | 40.206 | 97 | 57 | 1 | 1 | 97 | 1 | 96 | 1.53e-18 | 75.9 |
| MS.gene067641.t1 | MTR_7g055790 | 44.444 | 99 | 37 | 1 | 1 | 99 | 59 | 139 | 1.46e-17 | 72.8 |
| MS.gene067641.t1 | MTR_3g466930 | 36.765 | 68 | 43 | 0 | 32 | 99 | 2 | 69 | 8.46e-17 | 70.5 |
| MS.gene067641.t1 | MTR_3g466900 | 38.235 | 68 | 42 | 0 | 32 | 99 | 2 | 69 | 9.22e-17 | 70.5 |
| MS.gene067641.t1 | MTR_5g047560 | 34.343 | 99 | 48 | 2 | 1 | 99 | 1 | 82 | 2.47e-15 | 67.0 |
| MS.gene067641.t1 | MTR_1g114730 | 36.000 | 100 | 57 | 3 | 5 | 98 | 5 | 103 | 1.03e-14 | 68.2 |
| MS.gene067641.t1 | MTR_6g018920 | 37.000 | 100 | 56 | 4 | 5 | 98 | 5 | 103 | 1.11e-13 | 65.5 |
| MS.gene067641.t1 | MTR_6g005450 | 37.000 | 100 | 56 | 3 | 5 | 98 | 5 | 103 | 3.87e-13 | 63.9 |
| MS.gene067641.t1 | MTR_6g005440 | 36.000 | 100 | 57 | 3 | 5 | 98 | 5 | 103 | 4.27e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067641.t1 | AT5G48670 | 56.000 | 100 | 44 | 0 | 1 | 100 | 1 | 100 | 5.16e-30 | 108 |
| MS.gene067641.t1 | AT3G05860 | 43.000 | 100 | 57 | 0 | 1 | 100 | 1 | 100 | 8.15e-30 | 105 |
| MS.gene067641.t1 | AT3G05860 | 44.330 | 97 | 54 | 0 | 1 | 97 | 1 | 97 | 4.46e-29 | 105 |
| MS.gene067641.t1 | AT3G05860 | 44.330 | 97 | 54 | 0 | 1 | 97 | 1 | 97 | 6.31e-29 | 105 |
| MS.gene067641.t1 | AT5G26650 | 46.875 | 96 | 51 | 0 | 4 | 99 | 2 | 97 | 1.79e-27 | 103 |
| MS.gene067641.t1 | AT5G27960 | 46.875 | 96 | 51 | 0 | 4 | 99 | 2 | 97 | 4.45e-27 | 101 |
| MS.gene067641.t1 | AT1G65300 | 49.462 | 93 | 47 | 0 | 4 | 96 | 3 | 95 | 8.44e-27 | 99.8 |
| MS.gene067641.t1 | AT1G65330 | 46.739 | 92 | 49 | 0 | 5 | 96 | 4 | 95 | 2.93e-25 | 95.9 |
| MS.gene067641.t1 | AT5G27810 | 50.000 | 68 | 34 | 0 | 29 | 96 | 1 | 68 | 1.53e-21 | 82.4 |
| MS.gene067641.t1 | AT1G31630 | 40.206 | 97 | 55 | 2 | 5 | 101 | 4 | 97 | 5.60e-21 | 85.5 |
| MS.gene067641.t1 | AT5G26630 | 43.000 | 100 | 57 | 0 | 1 | 100 | 1 | 100 | 8.75e-21 | 82.8 |
| MS.gene067641.t1 | AT1G31640 | 38.947 | 95 | 57 | 1 | 5 | 99 | 4 | 97 | 1.17e-20 | 85.5 |
| MS.gene067641.t1 | AT2G28700 | 36.735 | 98 | 62 | 0 | 1 | 98 | 1 | 98 | 3.19e-20 | 83.2 |
| MS.gene067641.t1 | AT5G06500 | 39.216 | 102 | 62 | 0 | 1 | 102 | 1 | 102 | 9.57e-20 | 80.5 |
| MS.gene067641.t1 | AT1G22590 | 32.653 | 98 | 66 | 0 | 1 | 98 | 1 | 98 | 1.79e-19 | 78.2 |
| MS.gene067641.t1 | AT5G26580 | 39.394 | 99 | 46 | 1 | 1 | 99 | 1 | 85 | 3.57e-19 | 80.5 |
| MS.gene067641.t1 | AT2G40210 | 37.374 | 99 | 62 | 0 | 1 | 99 | 1 | 99 | 5.61e-18 | 77.4 |
| MS.gene067641.t1 | AT5G58890 | 38.462 | 91 | 46 | 3 | 1 | 81 | 1 | 91 | 1.16e-12 | 62.4 |
| MS.gene067641.t1 | AT5G55690 | 33.333 | 108 | 59 | 3 | 1 | 101 | 1 | 102 | 1.08e-11 | 59.7 |
| MS.gene067641.t1 | AT5G55690 | 33.333 | 108 | 59 | 3 | 1 | 101 | 1 | 102 | 1.08e-11 | 59.7 |
Find 27 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGCTTGTGCAGTAGTTTA+TGG | 0.155587 | 4.1:-69093907 | MS.gene067641:CDS |
| CACAACAATGACTGGGAAAA+AGG | 0.190222 | 4.1:-69094037 | None:intergenic |
| TCATTGTCGATGATGATTCT+AGG | 0.260008 | 4.1:-69094002 | MS.gene067641:CDS |
| AGATCTCTGGTTGAGGCTCA+TGG | 0.361064 | 4.1:+69093881 | None:intergenic |
| TGTGGTGATGGCCAGATCTC+TGG | 0.397343 | 4.1:+69093868 | None:intergenic |
| GGAAAGCAACATACAATAGA+AGG | 0.409123 | 4.1:-69093981 | MS.gene067641:CDS |
| GATCTCTGGTTGAGGCTCAT+GGG | 0.428787 | 4.1:+69093882 | None:intergenic |
| TCAAAATTCAGAACAATGAC+TGG | 0.434871 | 4.1:-69093826 | MS.gene067641:CDS |
| AAGAGACTTACATGAAAGAA+AGG | 0.463147 | 4.1:-69093774 | MS.gene067641:CDS |
| ATGAGCCTCAACCAGAGATC+TGG | 0.467494 | 4.1:-69093879 | MS.gene067641:CDS |
| ATCTCTGGTTGAGGCTCATG+GGG | 0.492174 | 4.1:+69093883 | None:intergenic |
| AGAGATCTGGCCATCACCAC+AGG | 0.494291 | 4.1:-69093866 | MS.gene067641:CDS |
| ATCACCACAGGGAGTTCAAA+CGG | 0.524260 | 4.1:-69093854 | MS.gene067641:CDS |
| AGAATCATCATCGACAATGA+AGG | 0.542377 | 4.1:+69094005 | None:intergenic |
| GATGGCCAGATCTCTGGTTG+AGG | 0.555241 | 4.1:+69093874 | None:intergenic |
| AACATACAATAGAAGGCGGA+AGG | 0.566279 | 4.1:-69093974 | MS.gene067641:CDS |
| GTTTGAACTCCCTGTGGTGA+TGG | 0.567517 | 4.1:+69093856 | None:intergenic |
| AGAGACTTACATGAAAGAAA+GGG | 0.577279 | 4.1:-69093773 | MS.gene067641:CDS |
| GCGGAAGGGTCTAATGATGA+AGG | 0.578779 | 4.1:-69093959 | MS.gene067641:CDS |
| TATGAACTAAGCACTTTGTG+TGG | 0.579542 | 4.1:-69093934 | MS.gene067641:CDS |
| CAAAATTCAGAACAATGACT+GGG | 0.592691 | 4.1:-69093825 | MS.gene067641:CDS |
| GCGGAAGAAGAGCAACAAAA+CGG | 0.630975 | 4.1:-69093803 | MS.gene067641:CDS |
| ACATACAATAGAAGGCGGAA+GGG | 0.677825 | 4.1:-69093973 | MS.gene067641:CDS |
| GAGATCTGGCCATCACCACA+GGG | 0.678612 | 4.1:-69093865 | MS.gene067641:CDS |
| AAGCAACATACAATAGAAGG+CGG | 0.694844 | 4.1:-69093978 | MS.gene067641:CDS |
| AATTCAGAACAATGACTGGG+CGG | 0.724414 | 4.1:-69093822 | MS.gene067641:CDS |
| AGCACCGTTTGAACTCCCTG+TGG | 0.787870 | 4.1:+69093850 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCTAAAAACTGAAGATACAA+AGG | - | chr4.1:69094025-69094044 | MS.gene067641:CDS | 25.0% |
| AAGAGACTTACATGAAAGAA+AGG | - | chr4.1:69094000-69094019 | MS.gene067641:CDS | 30.0% | |
| AGAGACTTACATGAAAGAAA+GGG | - | chr4.1:69094001-69094020 | MS.gene067641:CDS | 30.0% | |
| CAAAATTCAGAACAATGACT+GGG | - | chr4.1:69093949-69093968 | MS.gene067641:CDS | 30.0% | |
| TCAAAATTCAGAACAATGAC+TGG | - | chr4.1:69093948-69093967 | MS.gene067641:CDS | 30.0% | |
| AAGCAACATACAATAGAAGG+CGG | - | chr4.1:69093796-69093815 | MS.gene067641:CDS | 35.0% | |
| AGAATCATCATCGACAATGA+AGG | + | chr4.1:69093772-69093791 | None:intergenic | 35.0% | |
| GGAAAGCAACATACAATAGA+AGG | - | chr4.1:69093793-69093812 | MS.gene067641:CDS | 35.0% | |
| TATGAACTAAGCACTTTGTG+TGG | - | chr4.1:69093840-69093859 | MS.gene067641:CDS | 35.0% | |
| TCATTGTCGATGATGATTCT+AGG | - | chr4.1:69093772-69093791 | MS.gene067641:CDS | 35.0% | |
| AACATACAATAGAAGGCGGA+AGG | - | chr4.1:69093800-69093819 | MS.gene067641:CDS | 40.0% | |
| AATTCAGAACAATGACTGGG+CGG | - | chr4.1:69093952-69093971 | MS.gene067641:CDS | 40.0% | |
| ACATACAATAGAAGGCGGAA+GGG | - | chr4.1:69093801-69093820 | MS.gene067641:CDS | 40.0% | |
| GAAGCTTGTGCAGTAGTTTA+TGG | - | chr4.1:69093867-69093886 | MS.gene067641:CDS | 40.0% | |
| ATCACCACAGGGAGTTCAAA+CGG | - | chr4.1:69093920-69093939 | MS.gene067641:CDS | 45.0% | |
| GCGGAAGAAGAGCAACAAAA+CGG | - | chr4.1:69093971-69093990 | MS.gene067641:CDS | 45.0% | |
| AGATCTCTGGTTGAGGCTCA+TGG | + | chr4.1:69093896-69093915 | None:intergenic | 50.0% | |
| ATCTCTGGTTGAGGCTCATG+GGG | + | chr4.1:69093894-69093913 | None:intergenic | 50.0% | |
| ATGAGCCTCAACCAGAGATC+TGG | - | chr4.1:69093895-69093914 | MS.gene067641:CDS | 50.0% | |
| GATCTCTGGTTGAGGCTCAT+GGG | + | chr4.1:69093895-69093914 | None:intergenic | 50.0% | |
| GCGGAAGGGTCTAATGATGA+AGG | - | chr4.1:69093815-69093834 | MS.gene067641:CDS | 50.0% | |
| GTTTGAACTCCCTGTGGTGA+TGG | + | chr4.1:69093921-69093940 | None:intergenic | 50.0% | |
| AGAGATCTGGCCATCACCAC+AGG | - | chr4.1:69093908-69093927 | MS.gene067641:CDS | 55.0% | |
| AGCACCGTTTGAACTCCCTG+TGG | + | chr4.1:69093927-69093946 | None:intergenic | 55.0% | |
| GAGATCTGGCCATCACCACA+GGG | - | chr4.1:69093909-69093928 | MS.gene067641:CDS | 55.0% | |
| GATGGCCAGATCTCTGGTTG+AGG | + | chr4.1:69093903-69093922 | None:intergenic | 55.0% | |
| !! | TGTGGTGATGGCCAGATCTC+TGG | + | chr4.1:69093909-69093928 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 69093744 | 69094052 | 69093744 | ID=MS.gene067641 |
| chr4.1 | mRNA | 69093744 | 69094052 | 69093744 | ID=MS.gene067641.t1;Parent=MS.gene067641 |
| chr4.1 | exon | 69093744 | 69094052 | 69093744 | ID=MS.gene067641.t1.exon1;Parent=MS.gene067641.t1 |
| chr4.1 | CDS | 69093744 | 69094052 | 69093744 | ID=cds.MS.gene067641.t1;Parent=MS.gene067641.t1 |
| Gene Sequence |
| Protein sequence |
