Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067643.t1 | RHN76722.1 | 57.8 | 90 | 11 | 1 | 49 | 111 | 504 | 593 | 2.10E-16 | 95.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067643.t1 | Q9FJK3 | 51.3 | 76 | 33 | 1 | 4 | 79 | 3 | 74 | 2.0e-10 | 66.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067643.t1 | G7I3H5 | 57.8 | 90 | 11 | 1 | 49 | 111 | 920 | 1009 | 1.5e-16 | 95.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene067643.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067643.t1 | MTR_1g007990 | 53.535 | 99 | 19 | 1 | 40 | 111 | 911 | 1009 | 4.72e-25 | 98.6 |
| MS.gene067643.t1 | MTR_8g036130 | 80.000 | 45 | 9 | 0 | 4 | 48 | 3 | 47 | 3.19e-16 | 71.6 |
| MS.gene067643.t1 | MTR_4g032620 | 70.833 | 48 | 13 | 1 | 1 | 48 | 1 | 47 | 6.40e-15 | 68.2 |
| MS.gene067643.t1 | MTR_1g090783 | 54.839 | 62 | 24 | 1 | 4 | 65 | 2 | 59 | 1.10e-14 | 65.9 |
| MS.gene067643.t1 | MTR_1g090697 | 47.436 | 78 | 37 | 1 | 4 | 81 | 3 | 76 | 4.73e-14 | 64.3 |
| MS.gene067643.t1 | MTR_5g047580 | 54.839 | 62 | 24 | 1 | 4 | 65 | 3 | 60 | 7.25e-14 | 63.9 |
| MS.gene067643.t1 | MTR_4g028800 | 74.419 | 43 | 9 | 1 | 6 | 48 | 5 | 45 | 2.87e-13 | 60.1 |
| MS.gene067643.t1 | MTR_3g466830 | 68.889 | 45 | 14 | 0 | 4 | 48 | 2 | 46 | 2.92e-13 | 62.4 |
| MS.gene067643.t1 | MTR_1g077390 | 66.667 | 48 | 15 | 1 | 4 | 50 | 3 | 50 | 8.93e-13 | 61.2 |
| MS.gene067643.t1 | MTR_3g466890 | 68.889 | 45 | 14 | 0 | 4 | 48 | 2 | 46 | 1.24e-12 | 60.8 |
| MS.gene067643.t1 | MTR_2g016210 | 67.442 | 43 | 14 | 0 | 6 | 48 | 5 | 47 | 2.11e-12 | 61.6 |
| MS.gene067643.t1 | MTR_3g466980 | 62.222 | 45 | 17 | 0 | 4 | 48 | 3 | 47 | 5.84e-12 | 60.1 |
| MS.gene067643.t1 | MTR_5g075380 | 62.791 | 43 | 16 | 0 | 6 | 48 | 5 | 47 | 4.18e-11 | 56.6 |
| MS.gene067643.t1 | MTR_4g031910 | 82.222 | 45 | 8 | 0 | 4 | 48 | 3 | 47 | 5.04e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067643.t1 | AT3G05860 | 42.169 | 83 | 43 | 2 | 4 | 86 | 3 | 80 | 4.40e-13 | 62.8 |
| MS.gene067643.t1 | AT3G05860 | 42.169 | 83 | 43 | 2 | 4 | 86 | 3 | 80 | 5.60e-13 | 63.2 |
| MS.gene067643.t1 | AT3G05860 | 42.169 | 83 | 43 | 2 | 4 | 86 | 3 | 80 | 5.98e-13 | 63.2 |
| MS.gene067643.t1 | AT1G22590 | 36.134 | 119 | 62 | 5 | 1 | 110 | 1 | 114 | 6.86e-11 | 56.2 |
Find 25 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAACATTTCCGTAGCTTC+TGG | 0.330276 | 4.1:+69075835 | None:intergenic |
| TTACTACTGAAGATTGAAAA+AGG | 0.333620 | 4.1:-69075790 | MS.gene067643:intron |
| GAAGAAGAGTCTATTCAAAA+AGG | 0.366702 | 4.1:-69076060 | MS.gene067643:CDS |
| AGCATAGGTGTCCCAATCTC+TGG | 0.399969 | 4.1:+69075646 | None:intergenic |
| TTGTTAGAAAATCCAGAGAT+TGG | 0.426829 | 4.1:-69075658 | MS.gene067643:CDS |
| GGAAAGCAACATACAAGAAA+AGG | 0.449202 | 4.1:-69076082 | MS.gene067643:CDS |
| AACCTTACTTCTTCTACCCA+TGG | 0.464315 | 4.1:+69076136 | None:intergenic |
| TGTTAGAAAATCCAGAGATT+GGG | 0.467083 | 4.1:-69075657 | MS.gene067643:CDS |
| AGATACAACACCGAAGCCTT+TGG | 0.487832 | 4.1:-69075689 | MS.gene067643:CDS |
| ACAAGCCTCAATACCACTTA+GGG | 0.499751 | 4.1:+69076022 | None:intergenic |
| AACCATGGGTAGAAGAAGTA+AGG | 0.501192 | 4.1:-69076138 | None:intergenic |
| AGCAGCATCATTGACAATGA+AGG | 0.509472 | 4.1:+69076106 | None:intergenic |
| TAGCACCCTAAGTGGTATTG+AGG | 0.517240 | 4.1:-69076027 | MS.gene067643:CDS |
| CACAAGCCTCAATACCACTT+AGG | 0.534016 | 4.1:+69076021 | None:intergenic |
| AATGCCTTGACCAAAGGCTT+CGG | 0.537147 | 4.1:+69075679 | None:intergenic |
| TCTAACAATGCCTTGACCAA+AGG | 0.539314 | 4.1:+69075673 | None:intergenic |
| GGGACACCTATGCTAGACTT+AGG | 0.539982 | 4.1:-69075637 | MS.gene067643:CDS |
| GGATATTCCTCCAGAAGCTA+CGG | 0.564585 | 4.1:-69075845 | MS.gene067643:CDS |
| AACATTTCCGTAGCTTCTGG+AGG | 0.585851 | 4.1:+69075838 | None:intergenic |
| AACACCGAAGCCTTTGGTCA+AGG | 0.591331 | 4.1:-69075683 | MS.gene067643:CDS |
| CCACAAGGAAAATATATGCA+AGG | 0.593677 | 4.1:+69075877 | None:intergenic |
| GTTGAACTTAGCACCCTAAG+TGG | 0.600882 | 4.1:-69076035 | MS.gene067643:CDS |
| TCATTGTCAATGATGCTGCT+AGG | 0.603132 | 4.1:-69076103 | MS.gene067643:CDS |
| CAATCTCCTAAGTCTAGCAT+AGG | 0.607643 | 4.1:+69075631 | None:intergenic |
| ATATCCATCTTGTTCCCACA+AGG | 0.643932 | 4.1:+69075862 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAACAGAATAGTTAGA+TGG | + | chr4.1:69075862-69075881 | None:intergenic | 20.0% |
| !! | TTCGTATTTGTATTATGTAT+AGG | - | chr4.1:69075819-69075838 | MS.gene067643:CDS | 20.0% |
| !!! | ATCTAACTATTCTGTTTTTT+AGG | - | chr4.1:69075861-69075880 | MS.gene067643:CDS | 20.0% |
| ! | TTACTACTGAAGATTGAAAA+AGG | - | chr4.1:69075974-69075993 | MS.gene067643:intron | 25.0% |
| !! | GTATTATGTATAGGTTTTTG+AGG | - | chr4.1:69075828-69075847 | MS.gene067643:CDS | 25.0% |
| GAAGAAGAGTCTATTCAAAA+AGG | - | chr4.1:69075704-69075723 | MS.gene067643:intron | 30.0% | |
| TGTTAGAAAATCCAGAGATT+GGG | - | chr4.1:69076107-69076126 | MS.gene067643:CDS | 30.0% | |
| TTGTTAGAAAATCCAGAGAT+TGG | - | chr4.1:69076106-69076125 | MS.gene067643:CDS | 30.0% | |
| ! | CTTGCATATATTTTCCTTGT+GGG | - | chr4.1:69075888-69075907 | MS.gene067643:intron | 30.0% |
| CCACAAGGAAAATATATGCA+AGG | + | chr4.1:69075890-69075909 | None:intergenic | 35.0% | |
| GGAAAGCAACATACAAGAAA+AGG | - | chr4.1:69075682-69075701 | MS.gene067643:CDS | 35.0% | |
| ! | CCTTGCATATATTTTCCTTG+TGG | - | chr4.1:69075887-69075906 | MS.gene067643:intron | 35.0% |
| ACAAGCCTCAATACCACTTA+GGG | + | chr4.1:69075745-69075764 | None:intergenic | 40.0% | |
| AGCAGCATCATTGACAATGA+AGG | + | chr4.1:69075661-69075680 | None:intergenic | 40.0% | |
| ATATCCATCTTGTTCCCACA+AGG | + | chr4.1:69075905-69075924 | None:intergenic | 40.0% | |
| CAATCTCCTAAGTCTAGCAT+AGG | + | chr4.1:69076136-69076155 | None:intergenic | 40.0% | |
| GAAATGCAGAACTCTCGTAA+AGG | + | chr4.1:69075782-69075801 | None:intergenic | 40.0% | |
| TCATTGTCAATGATGCTGCT+AGG | - | chr4.1:69075661-69075680 | MS.gene067643:CDS | 40.0% | |
| TCTAACAATGCCTTGACCAA+AGG | + | chr4.1:69076094-69076113 | None:intergenic | 40.0% | |
| TTCAACATTTCCGTAGCTTC+TGG | + | chr4.1:69075932-69075951 | None:intergenic | 40.0% | |
| ! | TTTTCCTTGTGGGAACAAGA+TGG | - | chr4.1:69075898-69075917 | MS.gene067643:intron | 40.0% |
| AACATTTCCGTAGCTTCTGG+AGG | + | chr4.1:69075929-69075948 | None:intergenic | 45.0% | |
| AATGCCTTGACCAAAGGCTT+CGG | + | chr4.1:69076088-69076107 | None:intergenic | 45.0% | |
| AGATACAACACCGAAGCCTT+TGG | - | chr4.1:69076075-69076094 | MS.gene067643:CDS | 45.0% | |
| CACAAGCCTCAATACCACTT+AGG | + | chr4.1:69075746-69075765 | None:intergenic | 45.0% | |
| CTCGTAAAGGCCATACACTA+TGG | + | chr4.1:69075769-69075788 | None:intergenic | 45.0% | |
| GGATATTCCTCCAGAAGCTA+CGG | - | chr4.1:69075919-69075938 | MS.gene067643:intron | 45.0% | |
| GTTGAACTTAGCACCCTAAG+TGG | - | chr4.1:69075729-69075748 | MS.gene067643:intron | 45.0% | |
| TAGCACCCTAAGTGGTATTG+AGG | - | chr4.1:69075737-69075756 | MS.gene067643:intron | 45.0% | |
| AACACCGAAGCCTTTGGTCA+AGG | - | chr4.1:69076081-69076100 | MS.gene067643:CDS | 50.0% | |
| AGCATAGGTGTCCCAATCTC+TGG | + | chr4.1:69076121-69076140 | None:intergenic | 50.0% | |
| GAGGCTTGTGCCATAGTGTA+TGG | - | chr4.1:69075756-69075775 | MS.gene067643:intron | 50.0% | |
| GGGACACCTATGCTAGACTT+AGG | - | chr4.1:69076127-69076146 | MS.gene067643:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 69075630 | 69076156 | 69075630 | ID=MS.gene067643 |
| chr4.1 | mRNA | 69075630 | 69076156 | 69075630 | ID=MS.gene067643.t1;Parent=MS.gene067643 |
| chr4.1 | exon | 69076014 | 69076156 | 69076014 | ID=MS.gene067643.t1.exon1;Parent=MS.gene067643.t1 |
| chr4.1 | CDS | 69076014 | 69076156 | 69076014 | ID=cds.MS.gene067643.t1;Parent=MS.gene067643.t1 |
| chr4.1 | exon | 69075791 | 69075903 | 69075791 | ID=MS.gene067643.t1.exon2;Parent=MS.gene067643.t1 |
| chr4.1 | CDS | 69075791 | 69075903 | 69075791 | ID=cds.MS.gene067643.t1;Parent=MS.gene067643.t1 |
| chr4.1 | exon | 69075630 | 69075709 | 69075630 | ID=MS.gene067643.t1.exon3;Parent=MS.gene067643.t1 |
| chr4.1 | CDS | 69075630 | 69075709 | 69075630 | ID=cds.MS.gene067643.t1;Parent=MS.gene067643.t1 |
| Gene Sequence |
| Protein sequence |
