AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067692


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067692.t1 MTR_4g032620 90.476 231 22 0 1 231 1 231 1.05e-159 441
MS.gene067692.t1 MTR_8g036130 59.514 247 84 3 1 231 1 247 1.59e-94 277
MS.gene067692.t1 MTR_4g031910 53.361 238 95 2 1 231 1 229 6.19e-78 234
MS.gene067692.t1 MTR_4g032290 54.918 244 84 4 1 231 1 231 4.35e-74 224
MS.gene067692.t1 MTR_3g065100 48.117 239 112 3 1 228 1 238 6.68e-64 199
MS.gene067692.t1 MTR_4g032260 61.935 155 59 0 1 155 1 155 1.85e-56 177
MS.gene067692.t1 MTR_3g466980 42.672 232 117 5 1 227 1 221 2.64e-55 176
MS.gene067692.t1 MTR_2g035610 67.769 121 39 0 1 121 1 121 1.08e-49 159
MS.gene067692.t1 MTR_3g466830 43.827 162 88 2 3 163 2 161 5.26e-45 148
MS.gene067692.t1 MTR_3g466890 42.767 159 88 2 3 160 2 158 1.57e-39 134
MS.gene067692.t1 MTR_5g047580 40.000 155 91 2 1 155 1 153 1.38e-38 131
MS.gene067692.t1 MTR_5g075380 41.558 154 89 1 1 154 1 153 8.61e-38 129
MS.gene067692.t1 MTR_3g031100 41.935 155 90 0 1 155 1 155 2.23e-37 131
MS.gene067692.t1 MTR_4g063790 37.427 171 107 0 1 171 1 171 3.99e-37 128
MS.gene067692.t1 MTR_3g467080 37.419 155 97 0 1 155 1 155 6.62e-36 125
MS.gene067692.t1 MTR_2g016210 33.480 227 129 4 1 219 1 213 3.11e-35 125
MS.gene067692.t1 MTR_1g090783 41.497 147 84 2 3 149 2 146 3.82e-35 122
MS.gene067692.t1 MTR_7g011950 41.558 154 88 2 1 154 1 152 5.22e-35 122
MS.gene067692.t1 MTR_1g090697 36.709 158 98 2 1 158 1 156 6.37e-33 117
MS.gene067692.t1 MTR_4g028720 40.260 154 90 2 1 154 1 152 1.79e-32 115
MS.gene067692.t1 MTR_1g077390 37.013 154 95 2 1 154 1 152 5.11e-32 114
MS.gene067692.t1 MTR_4g028800 77.143 70 14 1 1 70 1 68 8.54e-32 111
MS.gene067692.t1 MTR_3g031240 35.976 164 101 2 1 162 1 162 1.00e-31 114
MS.gene067692.t1 MTR_1g090710 34.810 158 102 1 1 158 1 157 1.72e-31 113
MS.gene067692.t1 MTR_1g084950 38.710 155 93 2 1 155 1 153 3.29e-31 112
MS.gene067692.t1 MTR_1g077320 37.013 154 95 2 1 154 1 152 7.62e-29 106
MS.gene067692.t1 MTR_2g035580 39.355 155 93 1 1 155 1 154 1.73e-28 105
MS.gene067692.t1 MTR_3g466900 38.462 130 77 2 32 160 2 129 2.85e-28 104
MS.gene067692.t1 MTR_1g077300 40.127 157 84 3 1 154 1 150 3.56e-28 104
MS.gene067692.t1 MTR_3g466930 40.458 131 73 4 32 160 2 129 4.48e-28 103
MS.gene067692.t1 MTR_7g055800 47.899 119 34 2 1 119 1 91 1.49e-26 98.6
MS.gene067692.t1 MTR_3g465410 36.496 137 87 0 29 165 1 137 1.74e-25 97.4
MS.gene067692.t1 MTR_5g047560 38.983 118 55 2 1 118 1 101 4.00e-23 91.3
MS.gene067692.t1 MTR_4g032270 45.133 113 53 3 125 231 22 131 7.07e-23 90.5
MS.gene067692.t1 MTR_7g055790 48.649 111 39 1 1 111 59 151 3.48e-22 89.4
MS.gene067692.t1 MTR_7g055940 46.667 105 28 2 1 105 1 77 3.93e-21 84.3
MS.gene067692.t1 MTR_4g019670 31.847 157 105 1 3 157 6 162 1.01e-20 90.5
MS.gene067692.t1 MTR_7g106510 31.210 157 106 1 3 157 6 162 1.01e-20 90.5
MS.gene067692.t1 MTR_3g093900 37.398 123 75 2 3 124 6 127 1.42e-18 84.3
MS.gene067692.t1 MTR_2g035590 50.562 89 44 0 74 162 1 89 1.18e-17 75.9
MS.gene067692.t1 MTR_6g018920 30.000 160 97 6 1 154 1 151 1.81e-13 68.9
MS.gene067692.t1 MTR_1g114730 35.043 117 61 4 1 103 1 116 2.57e-13 68.6
MS.gene067692.t1 MTR_6g005450 35.593 118 61 4 1 104 1 117 2.61e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067692.t1 AT5G48670 52.795 161 75 1 1 161 1 160 4.91e-50 166
MS.gene067692.t1 AT1G65300 41.420 169 98 1 3 171 2 169 6.37e-40 139
MS.gene067692.t1 AT5G26650 40.462 173 100 2 4 176 2 171 6.18e-38 135
MS.gene067692.t1 AT5G26630 38.756 209 122 3 1 208 1 204 1.46e-37 130
MS.gene067692.t1 AT5G27960 39.306 173 102 2 4 176 2 171 4.30e-37 132
MS.gene067692.t1 AT3G05860 40.667 150 88 1 1 150 1 149 1.33e-36 128
MS.gene067692.t1 AT1G65330 41.875 160 92 1 3 162 2 160 2.13e-36 129
MS.gene067692.t1 AT3G05860 40.667 150 88 1 1 150 1 149 3.88e-36 128
MS.gene067692.t1 AT3G05860 40.667 150 88 1 1 150 1 149 4.97e-36 128
MS.gene067692.t1 AT5G27810 54.839 93 42 0 29 121 1 93 9.70e-33 115
MS.gene067692.t1 AT1G31630 35.220 159 101 2 3 161 2 158 8.97e-32 119
MS.gene067692.t1 AT1G31640 33.775 151 98 2 3 153 2 150 4.12e-29 114
MS.gene067692.t1 AT5G26580 36.774 155 83 2 1 155 1 140 4.76e-28 109
MS.gene067692.t1 AT2G28700 35.152 165 106 1 1 165 1 164 9.25e-27 105
MS.gene067692.t1 AT1G22590 38.614 101 62 0 1 101 1 101 5.47e-21 86.3
MS.gene067692.t1 AT2G40210 41.176 119 70 0 1 119 1 119 5.66e-19 84.7
MS.gene067692.t1 AT5G06500 30.519 154 105 1 1 154 1 152 2.52e-18 81.3
MS.gene067692.t1 AT5G58890 39.394 99 52 3 1 92 1 98 1.46e-12 65.9
MS.gene067692.t1 AT5G55690 38.298 94 51 2 1 87 1 94 3.60e-12 64.7
MS.gene067692.t1 AT5G55690 38.298 94 51 2 1 87 1 94 3.60e-12 64.7

Find 56 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGCCATTTCCTCATTCC+TGG 0.183624 4.1:-68394921 None:intergenic
AATGATGTCACCATTATTCA+TGG 0.300939 4.1:-68394969 None:intergenic
TTGTATCTTCAGTTTCTCTT+TGG 0.302597 4.1:-68394687 None:intergenic
ATTCATTCAAGGCAGTATAT+TGG 0.306143 4.1:-68395077 None:intergenic
TTCATTCAAGGCAGTATATT+GGG 0.306995 4.1:-68395076 None:intergenic
ATCTCTGGTTGAGGCTTGTA+TGG 0.342107 4.1:-68394545 None:intergenic
GAAGCATGTGCCATAGTCTA+TGG 0.364353 4.1:+68394521 MS.gene067692:CDS
GGAAAGCAACATACAATAAC+AGG 0.369187 4.1:+68394447 MS.gene067692:CDS
TATGCAGAGTAATATGTCAT+TGG 0.410310 4.1:+68394775 MS.gene067692:CDS
TGGTAGAAGGTTGGAAGAAG+AGG 0.420916 4.1:+68394847 MS.gene067692:CDS
ACAAGCCTCAACCAGAGATA+TGG 0.424604 4.1:+68394549 MS.gene067692:CDS
TCATTGTAAATGATGCATCT+AGG 0.432410 4.1:+68394426 MS.gene067692:CDS
AGGATGGGTCATGGCCATGC+TGG 0.433216 4.1:+68394941 MS.gene067692:CDS
ATGTTTCAATGTCTTTATGA+AGG 0.443108 4.1:+68394746 MS.gene067692:CDS
AAGAGACATATATGAAAGAA+AGG 0.443744 4.1:+68394654 MS.gene067692:CDS
GAAGATGAGACGGTGCCATT+TGG 0.449628 4.1:+68395031 MS.gene067692:CDS
AAATGAAGGAGGTTGGTAGA+AGG 0.451827 4.1:+68394834 MS.gene067692:CDS
GTCATCCAGGAATGAGGAAA+TGG 0.454775 4.1:+68394916 MS.gene067692:CDS
GAAGGAGGTTGGTAGAAGGT+TGG 0.465981 4.1:+68394838 MS.gene067692:CDS
TTGATTCAAAACATCTTTGT+TGG 0.470119 4.1:+68394798 MS.gene067692:CDS
ATGACAATGGTAGTGCCATT+TGG 0.472182 4.1:-68394898 None:intergenic
TTGGAAGAAGAGGATAATAA+TGG 0.472270 4.1:+68394857 MS.gene067692:CDS
ACATGAAATTCAACTCCAAA+TGG 0.474248 4.1:+68394883 MS.gene067692:CDS
TGTGATGACGGCCATATCTC+TGG 0.481006 4.1:-68394560 None:intergenic
TTGATGGGCTTGATGATGAA+TGG 0.481425 4.1:+68395004 MS.gene067692:CDS
GAATGAGGAAATGGCAAGGA+TGG 0.482915 4.1:+68394925 MS.gene067692:CDS
CTAGGATCGACCTCGCCAAA+TGG 0.502040 4.1:-68395046 None:intergenic
TGGCACTACCATTGTCATCC+AGG 0.504866 4.1:+68394903 MS.gene067692:CDS
GTTGATTGACCAGAAAATGA+AGG 0.505207 4.1:+68394820 MS.gene067692:CDS
ATTCATAACAATGACTGAAA+GGG 0.512113 4.1:+68394607 MS.gene067692:CDS
ATTTGGACTCCTTGTGATGA+CGG 0.513475 4.1:-68394572 None:intergenic
CTTGTATGGTCCATAGACTA+TGG 0.514753 4.1:-68394531 None:intergenic
GCTGGGATGACCATGAATAA+TGG 0.515101 4.1:+68394959 MS.gene067692:CDS
AATTCATAACAATGACTGAA+AGG 0.522955 4.1:+68394606 MS.gene067692:CDS
GGATGGGTCATGGCCATGCT+GGG 0.533577 4.1:+68394942 MS.gene067692:CDS
AGAGACATATATGAAAGAAA+GGG 0.542993 4.1:+68394655 MS.gene067692:CDS
ACCATTGTCATCCAGGAATG+AGG 0.554944 4.1:+68394910 MS.gene067692:CDS
GACGGCCATATCTCTGGTTG+AGG 0.560463 4.1:-68394554 None:intergenic
GACCAGAAAATGAAGGAGGT+TGG 0.561443 4.1:+68394827 MS.gene067692:CDS
AACATACAATAACAGGTGGA+AGG 0.581713 4.1:+68394454 MS.gene067692:CDS
GTGGAAGGGTCTACTGAAGA+AGG 0.590784 4.1:+68394469 MS.gene067692:CDS
AAGAGAAACTGAAGATACAA+AGG 0.595097 4.1:+68394690 MS.gene067692:CDS
GAGATATGGCCGTCATCACA+AGG 0.597681 4.1:+68394563 MS.gene067692:CDS
AATGAGGAAATGGCAAGGAT+GGG 0.613705 4.1:+68394926 MS.gene067692:CDS
GATACAAAGGCATGACAACA+AGG 0.619432 4.1:+68394703 MS.gene067692:CDS
TCCTCATTCCTGGATGACAA+TGG 0.622319 4.1:-68394911 None:intergenic
ACATACAATAACAGGTGGAA+GGG 0.624477 4.1:+68394455 MS.gene067692:CDS
GAAATGGCAAGGATGGGTCA+TGG 0.626584 4.1:+68394932 MS.gene067692:CDS
TGACAACAAGGAGAAAGAGA+TGG 0.637254 4.1:+68394715 MS.gene067692:CDS
AAGCAACATACAATAACAGG+TGG 0.643203 4.1:+68394450 MS.gene067692:CDS
GATTGACCAGAAAATGAAGG+AGG 0.644653 4.1:+68394823 MS.gene067692:CDS
TATTCATGGTCATCCCAGCA+TGG 0.650127 4.1:-68394955 None:intergenic
CCAGGAATGAGGAAATGGCA+AGG 0.651946 4.1:+68394921 MS.gene067692:CDS
TGAGACGGTGCCATTTGGCG+AGG 0.664323 4.1:+68395036 MS.gene067692:CDS
TATGAACTAAGCACTCTGTG+TGG 0.701795 4.1:+68394494 MS.gene067692:CDS
GAATGGTTATGAAGATGAGA+CGG 0.707575 4.1:+68395021 MS.gene067692:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGAAAATCAATTTTTGAT+GGG + chr4.1:68394989-68395008 MS.gene067692:CDS 15.0%
!!! CATTGAAAATCAATTTTTGA+TGG + chr4.1:68394988-68395007 MS.gene067692:CDS 20.0%
! AAGAGACATATATGAAAGAA+AGG + chr4.1:68394654-68394673 MS.gene067692:CDS 25.0%
! AATTCATAACAATGACTGAA+AGG + chr4.1:68394606-68394625 MS.gene067692:CDS 25.0%
! AGAGACATATATGAAAGAAA+GGG + chr4.1:68394655-68394674 MS.gene067692:CDS 25.0%
! ATGTTTCAATGTCTTTATGA+AGG + chr4.1:68394746-68394765 MS.gene067692:CDS 25.0%
! ATTCATAACAATGACTGAAA+GGG + chr4.1:68394607-68394626 MS.gene067692:CDS 25.0%
! TTGATTCAAAACATCTTTGT+TGG + chr4.1:68394798-68394817 MS.gene067692:CDS 25.0%
!!! TATGAAAGAAAGGGTTTTAA+AGG + chr4.1:68394664-68394683 MS.gene067692:CDS 25.0%
AAGAGAAACTGAAGATACAA+AGG + chr4.1:68394690-68394709 MS.gene067692:CDS 30.0%
AATGATGTCACCATTATTCA+TGG - chr4.1:68394972-68394991 None:intergenic 30.0%
ACATGAAATTCAACTCCAAA+TGG + chr4.1:68394883-68394902 MS.gene067692:CDS 30.0%
AGTATATTGGGCAAAAATCT+AGG - chr4.1:68395067-68395086 None:intergenic 30.0%
TATGCAGAGTAATATGTCAT+TGG + chr4.1:68394775-68394794 MS.gene067692:CDS 30.0%
TCATTGTAAATGATGCATCT+AGG + chr4.1:68394426-68394445 MS.gene067692:CDS 30.0%
TTGGAAGAAGAGGATAATAA+TGG + chr4.1:68394857-68394876 MS.gene067692:CDS 30.0%
TTGTATCTTCAGTTTCTCTT+TGG - chr4.1:68394690-68394709 None:intergenic 30.0%
!!! TGAATTTTGAGAGCACTATT+TGG - chr4.1:68394592-68394611 None:intergenic 30.0%
AACATACAATAACAGGTGGA+AGG + chr4.1:68394454-68394473 MS.gene067692:CDS 35.0%
AAGCAACATACAATAACAGG+TGG + chr4.1:68394450-68394469 MS.gene067692:CDS 35.0%
ACATACAATAACAGGTGGAA+GGG + chr4.1:68394455-68394474 MS.gene067692:CDS 35.0%
GAATGGTTATGAAGATGAGA+CGG + chr4.1:68395021-68395040 MS.gene067692:CDS 35.0%
GGAAAGCAACATACAATAAC+AGG + chr4.1:68394447-68394466 MS.gene067692:CDS 35.0%
GTTGATTGACCAGAAAATGA+AGG + chr4.1:68394820-68394839 MS.gene067692:CDS 35.0%
AATGAGGAAATGGCAAGGAT+GGG + chr4.1:68394926-68394945 MS.gene067692:CDS 40.0%
ATTTGGACTCCTTGTGATGA+CGG - chr4.1:68394575-68394594 None:intergenic 40.0%
CTTGTATGGTCCATAGACTA+TGG - chr4.1:68394534-68394553 None:intergenic 40.0%
GATTGACCAGAAAATGAAGG+AGG + chr4.1:68394823-68394842 MS.gene067692:CDS 40.0%
TATGAACTAAGCACTCTGTG+TGG + chr4.1:68394494-68394513 MS.gene067692:CDS 40.0%
TGACAACAAGGAGAAAGAGA+TGG + chr4.1:68394715-68394734 MS.gene067692:CDS 40.0%
! ATGACAATGGTAGTGCCATT+TGG - chr4.1:68394901-68394920 None:intergenic 40.0%
! GATACAAAGGCATGACAACA+AGG + chr4.1:68394703-68394722 MS.gene067692:CDS 40.0%
! TACCAACCTCCTTCATTTTC+TGG - chr4.1:68394832-68394851 None:intergenic 40.0%
!! AAATGAAGGAGGTTGGTAGA+AGG + chr4.1:68394834-68394853 MS.gene067692:CDS 40.0%
!! TTGATGGGCTTGATGATGAA+TGG + chr4.1:68395004-68395023 MS.gene067692:CDS 40.0%
ACAAGCCTCAACCAGAGATA+TGG + chr4.1:68394549-68394568 MS.gene067692:CDS 45.0%
ACCATTGTCATCCAGGAATG+AGG + chr4.1:68394910-68394929 MS.gene067692:CDS 45.0%
ATCTCTGGTTGAGGCTTGTA+TGG - chr4.1:68394548-68394567 None:intergenic 45.0%
GAAGCATGTGCCATAGTCTA+TGG + chr4.1:68394521-68394540 MS.gene067692:CDS 45.0%
GAATGAGGAAATGGCAAGGA+TGG + chr4.1:68394925-68394944 MS.gene067692:CDS 45.0%
GACCAGAAAATGAAGGAGGT+TGG + chr4.1:68394827-68394846 MS.gene067692:CDS 45.0%
GCTGGGATGACCATGAATAA+TGG + chr4.1:68394959-68394978 MS.gene067692:CDS 45.0%
GTCATCCAGGAATGAGGAAA+TGG + chr4.1:68394916-68394935 MS.gene067692:CDS 45.0%
TATTCATGGTCATCCCAGCA+TGG - chr4.1:68394958-68394977 None:intergenic 45.0%
TCCTCATTCCTGGATGACAA+TGG - chr4.1:68394914-68394933 None:intergenic 45.0%
TGGTAGAAGGTTGGAAGAAG+AGG + chr4.1:68394847-68394866 MS.gene067692:CDS 45.0%
CCAGGAATGAGGAAATGGCA+AGG + chr4.1:68394921-68394940 MS.gene067692:CDS 50.0%
GAAATGGCAAGGATGGGTCA+TGG + chr4.1:68394932-68394951 MS.gene067692:CDS 50.0%
GAGATATGGCCGTCATCACA+AGG + chr4.1:68394563-68394582 MS.gene067692:CDS 50.0%
GTGGAAGGGTCTACTGAAGA+AGG + chr4.1:68394469-68394488 MS.gene067692:CDS 50.0%
TGGCACTACCATTGTCATCC+AGG + chr4.1:68394903-68394922 MS.gene067692:CDS 50.0%
! CCTTGCCATTTCCTCATTCC+TGG - chr4.1:68394924-68394943 None:intergenic 50.0%
!! GAAGATGAGACGGTGCCATT+TGG + chr4.1:68395031-68395050 MS.gene067692:CDS 50.0%
!! GAAGGAGGTTGGTAGAAGGT+TGG + chr4.1:68394838-68394857 MS.gene067692:CDS 50.0%
!! TGTGATGACGGCCATATCTC+TGG - chr4.1:68394563-68394582 None:intergenic 50.0%
CTAGGATCGACCTCGCCAAA+TGG - chr4.1:68395049-68395068 None:intergenic 55.0%
GACGGCCATATCTCTGGTTG+AGG - chr4.1:68394557-68394576 None:intergenic 55.0%
AGGATGGGTCATGGCCATGC+TGG + chr4.1:68394941-68394960 MS.gene067692:CDS 60.0%
GGATGGGTCATGGCCATGCT+GGG + chr4.1:68394942-68394961 MS.gene067692:CDS 60.0%
!! TGAGACGGTGCCATTTGGCG+AGG + chr4.1:68395036-68395055 MS.gene067692:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 68394398 68395093 68394398 ID=MS.gene067692
chr4.1 mRNA 68394398 68395093 68394398 ID=MS.gene067692.t1;Parent=MS.gene067692
chr4.1 exon 68394398 68395093 68394398 ID=MS.gene067692.t1.exon1;Parent=MS.gene067692.t1
chr4.1 CDS 68394398 68395093 68394398 ID=cds.MS.gene067692.t1;Parent=MS.gene067692.t1
Gene Sequence

>MS.gene067692

ATGGCTAGGAAAAAGATGAAAATTGCTTTCATTGTAAATGATGCATCTAGGAAAGCAACATACAATAACAGGTGGAAGGGTCTACTGAAGAAGGTTTATGAACTAAGCACTCTGTGTGGAGTTGAAGCATGTGCCATAGTCTATGGACCATACAAGCCTCAACCAGAGATATGGCCGTCATCACAAGGAGTCCAAATAGTGCTCTCAAAATTCATAACAATGACTGAAAGGGATAAGAGCAAGAAAATGCTGAATCAAGAGACATATATGAAAGAAAGGGTTTTAAAGGCCAAAGAGAAACTGAAGATACAAAGGCATGACAACAAGGAGAAAGAGATGGCAATGCTCATGTTTCAATGTCTTTATGAAGGTAACTTTATGCAGAGTAATATGTCATTGGTTGATTCAAAACATCTTTGTTGGTTGATTGACCAGAAAATGAAGGAGGTTGGTAGAAGGTTGGAAGAAGAGGATAATAATGGTCAACATGAAATTCAACTCCAAATGGCACTACCATTGTCATCCAGGAATGAGGAAATGGCAAGGATGGGTCATGGCCATGCTGGGATGACCATGAATAATGGTGACATCATTGAAAATCAATTTTTGATGGGCTTGATGATGAATGGTTATGAAGATGAGACGGTGCCATTTGGCGAGGTCGATCCTAGATTTTTGCCCAATATACTGCCTTGA

Protein sequence

>MS.gene067692.t1

MARKKMKIAFIVNDASRKATYNNRWKGLLKKVYELSTLCGVEACAIVYGPYKPQPEIWPSSQGVQIVLSKFITMTERDKSKKMLNQETYMKERVLKAKEKLKIQRHDNKEKEMAMLMFQCLYEGNFMQSNMSLVDSKHLCWLIDQKMKEVGRRLEEEDNNGQHEIQLQMALPLSSRNEEMARMGHGHAGMTMNNGDIIENQFLMGLMMNGYEDETVPFGEVDPRFLPNILP