AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067718

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067718.t1 MTR_4g047580 53.971 491 98 8 1 416 99 536 3.06e-151 440
MS.gene067718.t1 MTR_4g049760 54.957 464 88 7 1 391 107 522 6.14e-142 416
MS.gene067718.t1 MTR_4g049790 48.980 490 114 8 1 416 132 559 6.31e-120 361
MS.gene067718.t1 MTR_2g088000 35.983 478 174 15 1 406 170 587 6.95e-62 211
MS.gene067718.t1 MTR_2g023930 30.786 458 158 16 1 370 144 530 3.27e-39 148
MS.gene067718.t1 MTR_7g110720 41.589 214 74 6 217 379 201 414 9.99e-38 142
MS.gene067718.t1 MTR_7g110720 41.589 214 74 6 217 379 174 387 1.05e-37 142
MS.gene067718.t1 MTR_3g053330 30.168 358 165 9 2 348 95 378 9.73e-37 140
MS.gene067718.t1 MTR_4g132430 63.551 107 29 1 210 306 105 211 1.02e-36 138
MS.gene067718.t1 MTR_4g132430 63.551 107 29 1 210 306 239 345 8.64e-36 138
MS.gene067718.t1 MTR_2g045360 54.000 100 36 1 217 306 196 295 1.74e-29 119
MS.gene067718.t1 MTR_7g009730 46.774 124 50 2 189 296 170 293 2.44e-28 117
MS.gene067718.t1 MTR_4g094412 54.369 103 37 1 217 309 228 330 7.12e-26 109
MS.gene067718.t1 MTR_4g094412 54.369 103 37 1 217 309 219 321 7.39e-26 109
MS.gene067718.t1 MTR_4g107970 55.263 76 24 1 217 282 163 238 9.24e-23 100
MS.gene067718.t1 MTR_4g107970 54.717 106 38 1 217 312 240 345 9.40e-23 101
MS.gene067718.t1 MTR_6g038890 74.468 47 12 0 210 256 166 212 7.89e-20 88.2
MS.gene067718.t1 MTR_5g044695 74.468 47 12 0 210 256 55 101 1.04e-18 81.6
MS.gene067718.t1 MTR_4g007060 25.424 177 118 2 209 371 105 281 9.78e-18 83.6
MS.gene067718.t1 MTR_4g007060 25.424 177 118 2 209 371 139 315 1.11e-17 84.0
MS.gene067718.t1 MTR_3g056100 35.537 121 66 2 202 310 241 361 1.23e-16 82.0
MS.gene067718.t1 MTR_3g056100 35.537 121 66 2 202 310 228 348 1.28e-16 82.0
MS.gene067718.t1 MTR_4g094588 37.143 105 57 1 218 313 164 268 1.90e-16 80.5
MS.gene067718.t1 MTR_3g056100 35.537 121 66 2 202 310 385 505 1.99e-16 82.0
MS.gene067718.t1 MTR_3g056100 35.537 121 66 2 202 310 372 492 2.23e-16 81.6
MS.gene067718.t1 MTR_5g042920 39.450 109 49 3 189 284 277 381 7.33e-16 79.7
MS.gene067718.t1 MTR_7g011820 38.776 98 51 1 208 296 386 483 7.82e-16 79.7
MS.gene067718.t1 MTR_7g109800 39.785 93 43 2 217 300 130 218 8.38e-15 75.1
MS.gene067718.t1 MTR_4g082580 37.391 115 58 2 214 319 260 369 2.39e-14 74.7
MS.gene067718.t1 MTR_5g074400 33.813 139 78 3 202 327 390 527 3.11e-14 75.1
MS.gene067718.t1 MTR_4g013260 46.154 78 33 1 214 282 318 395 3.16e-14 74.7
MS.gene067718.t1 MTR_5g094430 43.038 79 36 1 215 284 235 313 3.54e-14 74.3
MS.gene067718.t1 MTR_5g094430 43.038 79 36 1 215 284 213 291 3.78e-14 74.3
MS.gene067718.t1 MTR_1g086790 40.506 79 38 1 217 286 143 221 7.04e-14 72.8
MS.gene067718.t1 MTR_3g009470 44.304 79 35 1 215 284 478 556 7.25e-14 73.9
MS.gene067718.t1 MTR_5g094430 43.038 79 36 1 215 284 347 425 7.62e-14 73.9
MS.gene067718.t1 MTR_3g031220 42.500 80 37 1 214 284 347 426 1.14e-13 73.2
MS.gene067718.t1 MTR_2g033820 39.241 79 39 1 217 286 188 266 1.23e-13 72.4
MS.gene067718.t1 MTR_7g071120 45.000 80 35 1 214 284 306 385 1.43e-13 72.8
MS.gene067718.t1 MTR_7g079010 37.255 102 52 2 211 303 120 218 5.36e-13 68.6
MS.gene067718.t1 MTR_7g028415 45.714 70 29 1 221 281 82 151 5.90e-13 67.0
MS.gene067718.t1 MTR_7g028710 45.714 70 29 1 221 281 82 151 5.90e-13 67.0
MS.gene067718.t1 MTR_2g105060 38.824 85 37 3 214 283 71 155 9.56e-13 68.2
MS.gene067718.t1 MTR_2g105060 38.824 85 37 3 214 283 146 230 9.85e-13 69.3
MS.gene067718.t1 MTR_1g015140 35.354 99 55 1 191 280 61 159 1.48e-12 65.9
MS.gene067718.t1 MTR_7g080020 34.906 106 60 1 217 313 478 583 1.65e-12 70.1
MS.gene067718.t1 MTR_1g013760 28.743 167 96 5 217 372 125 279 1.66e-12 68.2
MS.gene067718.t1 MTR_1g013790 28.261 138 87 2 227 352 96 233 2.08e-12 67.4
MS.gene067718.t1 MTR_4g122530 40.541 74 35 1 218 282 194 267 2.80e-12 68.6
MS.gene067718.t1 MTR_7g080000 42.667 75 34 1 219 284 419 493 3.39e-12 68.9
MS.gene067718.t1 MTR_5g097490 43.421 76 34 1 217 283 237 312 3.69e-12 68.2
MS.gene067718.t1 MTR_3g104750 40.845 71 33 1 219 280 91 161 3.93e-12 64.7
MS.gene067718.t1 MTR_8g096730 37.500 80 41 1 215 285 53 132 4.71e-12 63.9
MS.gene067718.t1 MTR_3g106060 30.488 164 74 7 231 369 137 285 7.40e-12 66.2
MS.gene067718.t1 MTR_5g043880 41.558 77 36 1 212 279 104 180 1.34e-11 65.5
MS.gene067718.t1 MTR_7g100510 38.750 80 40 1 212 282 341 420 1.43e-11 66.2
MS.gene067718.t1 MTR_5g091390 38.462 78 39 1 218 286 143 220 1.85e-11 65.1
MS.gene067718.t1 MTR_7g109600 38.372 86 41 2 211 284 30 115 4.88e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067718.t1 AT4G04450 34.985 323 134 8 2 307 20 283 2.44e-42 155
MS.gene067718.t1 AT4G04450 34.985 323 134 8 2 307 114 377 6.38e-42 155
MS.gene067718.t1 AT1G62300 61.261 111 33 1 211 311 291 401 3.17e-34 134
MS.gene067718.t1 AT4G22070 64.634 82 19 1 211 282 276 357 1.64e-31 126
MS.gene067718.t1 AT4G22070 64.634 82 19 1 211 282 276 357 1.64e-31 126
MS.gene067718.t1 AT4G22070 64.634 82 19 1 211 282 276 357 1.64e-31 126
MS.gene067718.t1 AT4G22070 64.634 82 19 1 211 282 276 357 1.64e-31 126
MS.gene067718.t1 AT1G68150 41.509 159 70 4 201 336 201 359 2.65e-29 118
MS.gene067718.t1 AT4G01720 43.939 132 57 3 166 282 170 299 3.43e-28 116
MS.gene067718.t1 AT5G15130 56.667 90 29 1 217 296 170 259 5.06e-28 116
MS.gene067718.t1 AT5G15130 56.667 90 29 1 217 296 212 301 5.67e-28 116
MS.gene067718.t1 AT1G18860 30.584 291 148 10 7 282 48 299 2.94e-25 108
MS.gene067718.t1 AT1G69810 48.544 103 42 2 205 296 118 220 3.84e-23 100
MS.gene067718.t1 AT1G69810 48.544 103 42 2 205 296 176 278 5.74e-23 100
MS.gene067718.t1 AT2G03340 36.522 115 55 2 179 284 371 476 6.54e-16 80.1
MS.gene067718.t1 AT1G29860 35.714 112 57 2 217 319 121 226 1.46e-15 77.0
MS.gene067718.t1 AT4G18170 48.684 76 30 1 217 283 157 232 1.89e-15 77.4
MS.gene067718.t1 AT1G64000 41.053 95 43 2 214 299 96 186 2.11e-15 74.7
MS.gene067718.t1 AT1G13960 44.872 78 34 1 214 282 391 468 3.09e-15 78.2
MS.gene067718.t1 AT5G41570 39.394 99 47 2 218 307 84 178 3.14e-15 73.9
MS.gene067718.t1 AT1G13960 44.872 78 34 1 214 282 238 315 3.14e-15 77.0
MS.gene067718.t1 AT5G13080 47.222 72 29 1 219 281 54 125 6.44e-15 72.0
MS.gene067718.t1 AT5G46350 46.667 75 31 1 218 283 169 243 1.23e-14 75.1
MS.gene067718.t1 AT3G01080 40.476 84 41 1 208 282 282 365 2.11e-14 75.1
MS.gene067718.t1 AT3G01080 40.476 84 41 1 208 282 320 403 2.16e-14 75.5
MS.gene067718.t1 AT4G26640 43.038 79 36 1 215 284 188 266 4.34e-14 73.9
MS.gene067718.t1 AT4G26640 43.038 79 36 1 215 284 188 266 4.34e-14 73.9
MS.gene067718.t1 AT4G26640 43.038 79 36 1 215 284 188 266 4.34e-14 73.9
MS.gene067718.t1 AT4G26640 43.038 79 36 1 215 284 188 266 4.34e-14 73.9
MS.gene067718.t1 AT5G07100 39.796 98 43 2 205 286 107 204 4.59e-14 71.6
MS.gene067718.t1 AT5G07100 39.796 98 43 2 205 286 107 204 4.59e-14 71.6
MS.gene067718.t1 AT5G07100 39.796 98 43 2 205 286 107 204 4.59e-14 71.6
MS.gene067718.t1 AT5G07100 39.796 98 43 2 205 286 107 204 4.59e-14 71.6
MS.gene067718.t1 AT4G26640 43.038 79 36 1 215 284 292 370 6.63e-14 73.9
MS.gene067718.t1 AT4G26640 43.038 79 36 1 215 284 324 402 6.93e-14 73.9
MS.gene067718.t1 AT4G26640 43.038 79 36 1 215 284 364 442 7.38e-14 73.9
MS.gene067718.t1 AT2G38470 42.500 80 37 1 214 284 344 423 1.00e-13 73.6
MS.gene067718.t1 AT5G07100 39.796 98 43 2 205 286 200 297 1.08e-13 72.0
MS.gene067718.t1 AT1G80840 26.816 179 99 7 212 366 121 291 2.64e-13 70.9
MS.gene067718.t1 AT5G49520 39.241 79 39 1 217 286 206 284 2.96e-13 71.6
MS.gene067718.t1 AT5G56270 43.038 79 36 1 215 284 470 548 3.03e-13 72.4
MS.gene067718.t1 AT2G25000 29.319 191 106 4 106 285 16 188 3.07e-13 69.7
MS.gene067718.t1 AT2G30250 43.038 79 36 1 217 286 313 391 4.10e-13 70.9
MS.gene067718.t1 AT2G25000 35.955 89 47 1 207 285 78 166 8.44e-13 68.2
MS.gene067718.t1 AT2G25000 35.955 89 47 1 207 285 78 166 8.44e-13 68.2
MS.gene067718.t1 AT2G25000 35.955 89 47 1 207 285 121 209 1.29e-12 68.2
MS.gene067718.t1 AT3G62340 37.273 110 58 2 218 318 104 211 1.48e-12 68.2
MS.gene067718.t1 AT2G47260 39.024 82 41 1 217 289 159 240 1.68e-12 68.6
MS.gene067718.t1 AT3G01970 45.070 71 30 1 218 279 51 121 3.25e-12 64.3
MS.gene067718.t1 AT2G46130 37.363 91 44 2 218 299 16 102 3.31e-12 63.2
MS.gene067718.t1 AT2G04880 36.047 86 46 1 206 282 257 342 5.29e-12 67.8
MS.gene067718.t1 AT2G04880 36.047 86 46 1 206 282 281 366 6.44e-12 67.8
MS.gene067718.t1 AT5G26170 40.000 70 33 1 219 279 100 169 1.55e-11 63.2
MS.gene067718.t1 AT1G55600 41.333 75 35 1 219 284 294 368 1.93e-11 66.2
MS.gene067718.t1 AT4G31800 38.824 85 37 3 217 286 156 240 2.10e-11 65.1
MS.gene067718.t1 AT4G31800 38.824 85 37 3 217 286 91 175 2.13e-11 64.3
MS.gene067718.t1 AT5G52830 40.260 77 36 1 226 292 159 235 4.57e-11 64.3
MS.gene067718.t1 AT4G26440 40.260 77 37 1 217 284 357 433 6.36e-11 64.7
MS.gene067718.t1 AT1G69310 39.189 74 36 1 215 279 130 203 8.66e-11 63.2
MS.gene067718.t1 AT1G69310 39.189 74 36 1 215 279 130 203 8.66e-11 63.2
MS.gene067718.t1 AT1G69310 39.189 74 36 1 215 279 130 203 8.66e-11 63.2

Find 70 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATTTCTGAGGGGAAATT+TGG 0.148151 4.2:+48962546 MS.gene067718:CDS
GACAAAGATGCAAGAAAATT+TGG 0.170415 4.2:-48964548 None:intergenic
CTTTAACTTCCTTAAACTTA+TGG 0.217911 4.2:-48962404 None:intergenic
CCTGGTCTTACTTCCTTTAT+TGG 0.243665 4.2:+48964572 MS.gene067718:CDS
TTAACTGCCTGAGCAATATT+TGG 0.250376 4.2:-48964506 None:intergenic
GATGCTGGACCATAAGTTTA+AGG 0.279643 4.2:+48962395 MS.gene067718:CDS
CATAAGTTTAAGGAAGTTAA+AGG 0.283329 4.2:+48962405 MS.gene067718:CDS
TGCTGGATTGACTACTAATA+AGG 0.291693 4.2:+48962623 MS.gene067718:CDS
AATAAGGTTAAGGAAGTTAA+AGG 0.305864 4.2:+48962639 MS.gene067718:CDS
CGAGCTTGAACGAAAGAAAA+TGG 0.321409 4.2:+48962296 MS.gene067718:CDS
AAAAGGAAATTTATTGATGC+TGG 0.322652 4.2:+48962606 MS.gene067718:CDS
TCTTGGATCGACTACTAATA+TGG 0.352049 4.2:+48962503 MS.gene067718:CDS
TTATTGTGTTAGATCACTAA+TGG 0.353514 4.2:+48963052 MS.gene067718:intron
GGATGTCAATGGAGAAAGTA+TGG 0.358493 4.2:+48963073 MS.gene067718:CDS
AACTGAGAATGGTGGAGTAC+TGG 0.384618 4.2:+48962467 MS.gene067718:CDS
AATATGGTTAAGGAAGTTAA+AGG 0.385105 4.2:+48962519 MS.gene067718:CDS
TCTTTGGCTGCTGTAGGTAA+AGG 0.399359 4.2:-48964134 None:intergenic
TGGCTGAGTATGACCAATAA+AGG 0.401693 4.2:-48964585 None:intergenic
CTTTGGCTGCTGTAGGTAAA+GGG 0.407283 4.2:-48964133 None:intergenic
TTTCAGATGGCTATTCTTCA+AGG 0.408471 4.2:+48962273 None:intergenic
AAAGGAAAAGAAGTCTTTGA+TGG 0.410843 4.2:+48962423 MS.gene067718:CDS
ATAAAAGCAATGTTGTTGTT+TGG 0.416607 4.2:-48964605 None:intergenic
TAAATCTTATTATCGAAAAC+AGG 0.434395 4.2:+48963126 MS.gene067718:CDS
ATTGACTACTAATAAGGTTA+AGG 0.438970 4.2:+48962629 MS.gene067718:CDS
TAACATCCACATTCTTACTA+AGG 0.444417 4.2:-48962875 None:intergenic
CGATAATAAGATTTAGGACA+TGG 0.445164 4.2:-48963118 None:intergenic
ATCGACTACTAATATGGTTA+AGG 0.451220 4.2:+48962509 MS.gene067718:CDS
ACCTACAGCAGCCAAAGAAA+TGG 0.462971 4.2:+48964139 MS.gene067718:CDS
GCTAGAAGTCAGGACCAGTT+AGG 0.466887 4.2:+48962810 MS.gene067718:CDS
GCTATCATTGTGATTTCCTT+CGG 0.473387 4.2:-48964654 None:intergenic
GAGAAAGAGAGAACTGAGAA+TGG 0.485779 4.2:+48962456 MS.gene067718:CDS
TTTCTTGCATCTTTGTCACC+TGG 0.487925 4.2:+48964554 MS.gene067718:CDS
CTGGGACCGAGGAAAGATCT+TGG 0.499892 4.2:+48962486 MS.gene067718:CDS
ATGGTGGAGAATTAATGAAA+AGG 0.514995 4.2:+48962589 MS.gene067718:CDS
AATGAGAAAAGCTAGAAGTC+AGG 0.518607 4.2:+48962800 MS.gene067718:CDS
ACTGAGAATGGTGGAGTACT+GGG 0.520142 4.2:+48962468 MS.gene067718:CDS
GTCGTAATCACAACTTATGA+AGG 0.532383 4.2:+48964098 MS.gene067718:CDS
GGTACTACTAATAATTGTGA+AGG 0.533946 4.2:-48964485 None:intergenic
AGTAGTACCAAATATTGCTC+AGG 0.534861 4.2:+48964499 MS.gene067718:CDS
AAAATTTGGATTAGCAGCAA+TGG 0.542627 4.2:-48964534 None:intergenic
AAAGAGAGAACTGAGAATGG+TGG 0.543049 4.2:+48962459 MS.gene067718:CDS
ATGGTGGAGTACTGGGACCG+AGG 0.545279 4.2:+48962475 MS.gene067718:CDS
TTCAAATTTAACAGGTACAA+AGG 0.551833 4.2:+48964057 MS.gene067718:intron
GTGTTTCGAGAAAGTGATGC+TGG 0.554643 4.2:+48962380 MS.gene067718:CDS
CCAATAAAGGAAGTAAGACC+AGG 0.560382 4.2:-48964572 None:intergenic
GCCATTTCTTTGGCTGCTGT+AGG 0.560478 4.2:-48964140 None:intergenic
AAGCATCGAGGTTTCTTCGA+AGG 0.562333 4.2:+48962845 MS.gene067718:CDS
ACTTGAGAATGGTGAAGAGT+TGG 0.570463 4.2:+48962701 MS.gene067718:CDS
AGATCACTAATGGATGTCAA+TGG 0.573212 4.2:+48963062 MS.gene067718:intron
AAAGGACAAGAAATTTCTGA+GGG 0.575843 4.2:+48962537 MS.gene067718:CDS
AATAACAATGGAAATATCAC+AGG 0.579865 4.2:+48964698 MS.gene067718:CDS
AGCAACAATAATGTCACAGA+AGG 0.584455 4.2:+48964674 MS.gene067718:CDS
TAAAGGACAAGAAATTTCTG+AGG 0.584954 4.2:+48962536 MS.gene067718:CDS
GTTAGTCCTTAGTAAGAATG+TGG 0.588394 4.2:+48962869 MS.gene067718:CDS
GTACTACTAATAATTGTGAA+GGG 0.595394 4.2:-48964484 None:intergenic
AGCAACAATAATGTCACAGA+AGG 0.601849 4.2:+48964722 MS.gene067718:CDS
GTCGATCCAAGATCTTTCCT+CGG 0.604399 4.2:-48962492 None:intergenic
GTCACAGAAGGCAATAACAA+TGG 0.605929 4.2:+48964686 MS.gene067718:CDS
CAAATGTCAAAATATGCAGA+TGG 0.607406 4.2:+48964461 MS.gene067718:CDS
GATAATAAGATTTAGGACAT+GGG 0.612649 4.2:-48963117 None:intergenic
AAGTCAGGACCAGTTAGGAT+CGG 0.622675 4.2:+48962815 MS.gene067718:CDS
TGATAATGTTGAGGCAACAT+TGG 0.625126 4.2:+48962902 MS.gene067718:CDS
TGTTAATGTTGATAATGTTG+AGG 0.644357 4.2:+48962893 MS.gene067718:CDS
AGCTAGATCAGAAGAAACCA+TGG 0.644714 4.2:+48962947 MS.gene067718:CDS
TCAGCATCAACATCAAGCAA+TGG 0.645156 4.2:+48964197 MS.gene067718:CDS
AATGGCAACAATGTCACCGA+AGG 0.661836 4.2:+48964638 MS.gene067718:CDS
TGGCTGCTGTAGGTAAAGGG+TGG 0.666695 4.2:-48964130 None:intergenic
TACTAATGTTGAGACTACGA+TGG 0.681495 4.2:+48962755 MS.gene067718:CDS
AAGGACAAGAAATTTCTGAG+GGG 0.753560 4.2:+48962538 MS.gene067718:CDS
ATCGGCGATGAAAAGCATCG+AGG 0.769108 4.2:+48962833 MS.gene067718:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTAAAATACTTATGAAA+TGG + chr4.2:48964001-48964020 MS.gene067718:intron 10.0%
!! AAACTAATGATTAAATAACA+AGG - chr4.2:48963625-48963644 None:intergenic 15.0%
!! TAATACAATTCAAATTTAAC+AGG + chr4.2:48964049-48964068 MS.gene067718:intron 15.0%
!!! ATGCATATTTTGATTTTTAA+TGG - chr4.2:48963859-48963878 None:intergenic 15.0%
!! AATAAATTCTTTAAGCCAAA+AGG - chr4.2:48963031-48963050 None:intergenic 20.0%
!! TAAATCTTATTATCGAAAAC+AGG + chr4.2:48963126-48963145 MS.gene067718:CDS 20.0%
!!! AAAGGAAAAGAAGTTTTTAA+TGG + chr4.2:48962657-48962676 MS.gene067718:CDS 20.0%
!!! AACAATGTCAAAATCTTTTT+TGG + chr4.2:48964749-48964768 MS.gene067718:CDS 20.0%
!!! AATTTTGATTGGTTTTGATT+AGG - chr4.2:48964431-48964450 None:intergenic 20.0%
!!! TGTTTTCGATAATAAGATTT+AGG - chr4.2:48963127-48963146 None:intergenic 20.0%
! AAAAGGAAATTTATTGATGC+TGG + chr4.2:48962606-48962625 MS.gene067718:CDS 25.0%
! AATAACAATGGAAATATCAC+AGG + chr4.2:48964698-48964717 MS.gene067718:CDS 25.0%
! AATAAGGTTAAGGAAGTTAA+AGG + chr4.2:48962639-48962658 MS.gene067718:CDS 25.0%
! AATATGGTTAAGGAAGTTAA+AGG + chr4.2:48962519-48962538 MS.gene067718:CDS 25.0%
! ATTGACTACTAATAAGGTTA+AGG + chr4.2:48962629-48962648 MS.gene067718:CDS 25.0%
! CATAAGTTTAAGGAAGTTAA+AGG + chr4.2:48962405-48962424 MS.gene067718:CDS 25.0%
! GTACTACTAATAATTGTGAA+GGG - chr4.2:48964487-48964506 None:intergenic 25.0%
! GTATAATCAAGTGTAATAGT+TGG - chr4.2:48964308-48964327 None:intergenic 25.0%
! TCAAAATATGCATTGAAGTT+TGG + chr4.2:48963866-48963885 MS.gene067718:intron 25.0%
! TTATTGTGTTAGATCACTAA+TGG + chr4.2:48963052-48963071 MS.gene067718:intron 25.0%
! TTCAAATTTAACAGGTACAA+AGG + chr4.2:48964057-48964076 MS.gene067718:intron 25.0%
!! ATAAAAGCAATGTTGTTGTT+TGG - chr4.2:48964608-48964627 None:intergenic 25.0%
!! CAAAACCAATCAAAATTTTC+TGG + chr4.2:48964434-48964453 MS.gene067718:CDS 25.0%
!! CTTTAACTTCCTTAAACTTA+TGG - chr4.2:48962407-48962426 None:intergenic 25.0%
!! GATAATAAGATTTAGGACAT+GGG - chr4.2:48963120-48963139 None:intergenic 25.0%
!!! GCTTTTATTGATAGTAACAA+TGG + chr4.2:48964620-48964639 MS.gene067718:CDS 25.0%
!!! TGTTAATGTTGATAATGTTG+AGG + chr4.2:48962893-48962912 MS.gene067718:CDS 25.0%
AAAAAGAGAATGAAGAATGG+TGG + chr4.2:48962573-48962592 MS.gene067718:CDS 30.0%
AAAGGAAAAGAAGTCTTTGA+TGG + chr4.2:48962423-48962442 MS.gene067718:CDS 30.0%
AAAGGACAAGAAATTTCTGA+GGG + chr4.2:48962537-48962556 MS.gene067718:CDS 30.0%
AAATACTTGAAGAACAAGGA+AGG - chr4.2:48964259-48964278 None:intergenic 30.0%
AAGAAAAAGAGACTTGAGAA+TGG + chr4.2:48962690-48962709 MS.gene067718:CDS 30.0%
AATCTTCAAATACTCCACAA+AGG + chr4.2:48964330-48964349 MS.gene067718:intron 30.0%
ATAATGTGAAAGCAATCACT+AGG - chr4.2:48963474-48963493 None:intergenic 30.0%
ATCGACTACTAATATGGTTA+AGG + chr4.2:48962509-48962528 MS.gene067718:CDS 30.0%
ATGTACACTTTAACATACCA+TGG - chr4.2:48962967-48962986 None:intergenic 30.0%
CAAATGTCAAAATATGCAGA+TGG + chr4.2:48964461-48964480 MS.gene067718:CDS 30.0%
CAACAAACTCAAATCGAAAA+TGG + chr4.2:48963962-48963981 MS.gene067718:intron 30.0%
CAGAAAAAGAGAATGAAGAA+TGG + chr4.2:48962570-48962589 MS.gene067718:CDS 30.0%
GACAAAGATGCAAGAAAATT+TGG - chr4.2:48964551-48964570 None:intergenic 30.0%
GGTACTACTAATAATTGTGA+AGG - chr4.2:48964488-48964507 None:intergenic 30.0%
GTTGAAATACTTGAAGAACA+AGG - chr4.2:48964263-48964282 None:intergenic 30.0%
TAAAGGACAAGAAATTTCTG+AGG + chr4.2:48962536-48962555 MS.gene067718:CDS 30.0%
TAACATCCACATTCTTACTA+AGG - chr4.2:48962878-48962897 None:intergenic 30.0%
TAATGTGAAAGCAATCACTA+GGG - chr4.2:48963473-48963492 None:intergenic 30.0%
TCTAAGAGATTAAAGGAGTT+TGG - chr4.2:48964370-48964389 None:intergenic 30.0%
! ATGGTGGAGAATTAATGAAA+AGG + chr4.2:48962589-48962608 MS.gene067718:CDS 30.0%
! CATGTTTCTATGCTAGAAAA+TGG + chr4.2:48963777-48963796 MS.gene067718:intron 30.0%
! CGATAATAAGATTTAGGACA+TGG - chr4.2:48963121-48963140 None:intergenic 30.0%
! TAAATGCATGGATTGTGAAA+TGG - chr4.2:48963816-48963835 None:intergenic 30.0%
! TCTATGCTAGAAAATGGATT+TGG + chr4.2:48963783-48963802 MS.gene067718:intron 30.0%
!! AAAATTTGGATTAGCAGCAA+TGG - chr4.2:48964537-48964556 None:intergenic 30.0%
!! AACGTAGGGAGTATTTATTA+AGG + chr4.2:48963431-48963450 MS.gene067718:intron 30.0%
!! AGAAACATGAGCTTTGAAAA+TGG - chr4.2:48963766-48963785 None:intergenic 30.0%
!! GTAGTCCAGAAAATTTTGAT+TGG - chr4.2:48964442-48964461 None:intergenic 30.0%
!! TTGCGACATGTATTTTGAAA+CGG - chr4.2:48963192-48963211 None:intergenic 30.0%
!!! AGTTGTTTTTGCCATTTCTT+TGG - chr4.2:48964153-48964172 None:intergenic 30.0%
!!! ATGATAACTTGTTGTCCTTT+TGG + chr4.2:48963013-48963032 MS.gene067718:intron 30.0%
!!! GACATGTATTTTGAAACGTA+GGG + chr4.2:48963417-48963436 MS.gene067718:intron 30.0%
!!! GGTTTTGATTAGGTATTTGA+GGG - chr4.2:48964421-48964440 None:intergenic 30.0%
!!! TGGTTTTGATTAGGTATTTG+AGG - chr4.2:48964422-48964441 None:intergenic 30.0%
AAGGACAAGAAATTTCTGAG+GGG + chr4.2:48962538-48962557 MS.gene067718:CDS 35.0%
AATGAGAAAAGCTAGAAGTC+AGG + chr4.2:48962800-48962819 MS.gene067718:CDS 35.0%
ACAGTGATCTAGGAAACATT+CGG - chr4.2:48963580-48963599 None:intergenic 35.0%
AGCAACAATAATGTCACAGA+AGG + chr4.2:48964674-48964693 MS.gene067718:CDS 35.0%
AGTAGTACCAAATATTGCTC+AGG + chr4.2:48964499-48964518 MS.gene067718:CDS 35.0%
GCTATCATTGTGATTTCCTT+CGG - chr4.2:48964657-48964676 None:intergenic 35.0%
GTCGTAATCACAACTTATGA+AGG + chr4.2:48964098-48964117 MS.gene067718:CDS 35.0%
GTTAGTCCTTAGTAAGAATG+TGG + chr4.2:48962869-48962888 MS.gene067718:CDS 35.0%
TACTAATGTTGAGACTACGA+TGG + chr4.2:48962755-48962774 MS.gene067718:CDS 35.0%
TATGGACAAAAACTGTCGAA+AGG + chr4.2:48963091-48963110 MS.gene067718:CDS 35.0%
TATTTGATATGCATTGCACG+AGG + chr4.2:48963706-48963725 MS.gene067718:intron 35.0%
TCTTGGATCGACTACTAATA+TGG + chr4.2:48962503-48962522 MS.gene067718:CDS 35.0%
TGCTGGATTGACTACTAATA+AGG + chr4.2:48962623-48962642 MS.gene067718:CDS 35.0%
TTAACTGCCTGAGCAATATT+TGG - chr4.2:48964509-48964528 None:intergenic 35.0%
! AGATCACTAATGGATGTCAA+TGG + chr4.2:48963062-48963081 MS.gene067718:intron 35.0%
! GAAATTTCTGAGGGGAAATT+TGG + chr4.2:48962546-48962565 MS.gene067718:CDS 35.0%
! GCAGAACTCTAAGAGATTAA+AGG - chr4.2:48964377-48964396 None:intergenic 35.0%
! TGATAATGTTGAGGCAACAT+TGG + chr4.2:48962902-48962921 MS.gene067718:CDS 35.0%
!!! CGACATGTATTTTGAAACGT+AGG + chr4.2:48963416-48963435 MS.gene067718:intron 35.0%
!!! GACATGTATTTTGAAACGGA+GGG - chr4.2:48963188-48963207 None:intergenic 35.0%
!!! TCTAGCTTTTCTCATTGATG+AGG - chr4.2:48962796-48962815 None:intergenic 35.0%
AAAGAGAGAACTGAGAATGG+TGG + chr4.2:48962459-48962478 MS.gene067718:CDS 40.0%
ACTTGAGAATGGTGAAGAGT+TGG + chr4.2:48962701-48962720 MS.gene067718:CDS 40.0%
AGCTAGATCAGAAGAAACCA+TGG + chr4.2:48962947-48962966 MS.gene067718:CDS 40.0%
ATACTTACTACGTGAATGCC+TGG + chr4.2:48963927-48963946 MS.gene067718:intron 40.0%
ATGGTAGTTGGATCGAAGAT+TGG - chr4.2:48963897-48963916 None:intergenic 40.0%
CCAATAAAGGAAGTAAGACC+AGG - chr4.2:48964575-48964594 None:intergenic 40.0%
CGAGCTTGAACGAAAGAAAA+TGG + chr4.2:48962296-48962315 MS.gene067718:CDS 40.0%
GAGAAAGAGAGAACTGAGAA+TGG + chr4.2:48962456-48962475 MS.gene067718:CDS 40.0%
GATGCTGGACCATAAGTTTA+AGG + chr4.2:48962395-48962414 MS.gene067718:CDS 40.0%
GGATGTCAATGGAGAAAGTA+TGG + chr4.2:48963073-48963092 MS.gene067718:CDS 40.0%
GTCACAGAAGGCAATAACAA+TGG + chr4.2:48964686-48964705 MS.gene067718:CDS 40.0%
TCAGCATCAACATCAAGCAA+TGG + chr4.2:48964197-48964216 MS.gene067718:CDS 40.0%
TGGCTGAGTATGACCAATAA+AGG - chr4.2:48964588-48964607 None:intergenic 40.0%
TTTAATGGCCATCAACGTGA+TGG - chr4.2:48963844-48963863 None:intergenic 40.0%
TTTCTTGCATCTTTGTCACC+TGG + chr4.2:48964554-48964573 MS.gene067718:CDS 40.0%
! AAAGGAGTTTGGAACTGATC+TGG - chr4.2:48964359-48964378 None:intergenic 40.0%
! AAGGAGTTTGGAACTGATCT+GGG - chr4.2:48964358-48964377 None:intergenic 40.0%
! CATGTTTCTGACAGTGATCT+AGG - chr4.2:48963590-48963609 None:intergenic 40.0%
!! CCTGGTCTTACTTCCTTTAT+TGG + chr4.2:48964572-48964591 MS.gene067718:CDS 40.0%
!!! CGACATGTATTTTGAAACGG+AGG - chr4.2:48963189-48963208 None:intergenic 40.0%
AACTGATCTGGGTTCCTTTG+TGG - chr4.2:48964347-48964366 None:intergenic 45.0%
AAGTCAGGACCAGTTAGGAT+CGG + chr4.2:48962815-48962834 MS.gene067718:CDS 45.0%
AATGGCAACAATGTCACCGA+AGG + chr4.2:48964638-48964657 MS.gene067718:CDS 45.0%
ACCTACAGCAGCCAAAGAAA+TGG + chr4.2:48964139-48964158 MS.gene067718:CDS 45.0%
AGTAAGTATGTCGACGTCGA+TGG - chr4.2:48963916-48963935 None:intergenic 45.0%
CTTTGGCTGCTGTAGGTAAA+GGG - chr4.2:48964136-48964155 None:intergenic 45.0%
GAAAATGGTAGCTGCTAGCT+TGG + chr4.2:48963977-48963996 MS.gene067718:intron 45.0%
GCATCAACATCAAGCAATGG+TGG + chr4.2:48964200-48964219 MS.gene067718:intron 45.0%
GTAATAGTTGGAAACGACGC+AGG - chr4.2:48964296-48964315 None:intergenic 45.0%
GTCGATCCAAGATCTTTCCT+CGG - chr4.2:48962495-48962514 None:intergenic 45.0%
GTGTTTCGAGAAAGTGATGC+TGG + chr4.2:48962380-48962399 MS.gene067718:CDS 45.0%
TCTTTGGCTGCTGTAGGTAA+AGG - chr4.2:48964137-48964156 None:intergenic 45.0%
TGATATGCATTGCACGAGGT+TGG + chr4.2:48963710-48963729 MS.gene067718:intron 45.0%
TTACGCATCCATCACGTTGA+TGG + chr4.2:48963833-48963852 MS.gene067718:intron 45.0%
TTGAGGGATCAAAGAAGCTG+AGG - chr4.2:48964405-48964424 None:intergenic 45.0%
TTGTTGCATTCAGCGAATCC+AGG - chr4.2:48963948-48963967 None:intergenic 45.0%
! AACTGAGAATGGTGGAGTAC+TGG + chr4.2:48962467-48962486 MS.gene067718:CDS 45.0%
! ACTGAGAATGGTGGAGTACT+GGG + chr4.2:48962468-48962487 MS.gene067718:CDS 45.0%
! GTGTATTGACATGTGTGCAG+TGG + chr4.2:48963386-48963405 MS.gene067718:intron 45.0%
! TTTTCATCGCCGATCCTAAC+TGG - chr4.2:48962827-48962846 None:intergenic 45.0%
!! AAGCATCGAGGTTTCTTCGA+AGG + chr4.2:48962845-48962864 MS.gene067718:CDS 45.0%
!! GTGATGGATGCGTAAATGCA+TGG - chr4.2:48963828-48963847 None:intergenic 45.0%
!! TATATAATTAAAGATATTAA+TGG + chr4.2:48963355-48963374 MS.gene067718:intron 5.0%
!! TATATTAGTTAAAAAAAATA+TGG - chr4.2:48963251-48963270 None:intergenic 5.0%
ATGTCGACGTCGATGGTAGT+TGG - chr4.2:48963909-48963928 None:intergenic 50.0%
GCCATTTCTTTGGCTGCTGT+AGG - chr4.2:48964143-48964162 None:intergenic 50.0%
GCTAGAAGTCAGGACCAGTT+AGG + chr4.2:48962810-48962829 MS.gene067718:CDS 50.0%
!! ACAAGGAAGGAGTGCTCTTG+TGG - chr4.2:48964246-48964265 None:intergenic 50.0%
!! ATCGGCGATGAAAAGCATCG+AGG + chr4.2:48962833-48962852 MS.gene067718:CDS 50.0%
!! CAAGGAAGGAGTGCTCTTGT+GGG - chr4.2:48964245-48964264 None:intergenic 50.0%
CTGGGACCGAGGAAAGATCT+TGG + chr4.2:48962486-48962505 MS.gene067718:CDS 55.0%
TGGCTGCTGTAGGTAAAGGG+TGG - chr4.2:48964133-48964152 None:intergenic 55.0%
!! ATGGTGGAGTACTGGGACCG+AGG + chr4.2:48962475-48962494 MS.gene067718:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 48962279 48964784 48962279 ID=MS.gene067718
chr4.2 mRNA 48962279 48964784 48962279 ID=MS.gene067718.t1;Parent=MS.gene067718
chr4.2 exon 48962279 48962968 48962279 ID=MS.gene067718.t1.exon1;Parent=MS.gene067718.t1
chr4.2 CDS 48962279 48962968 48962279 ID=cds.MS.gene067718.t1;Parent=MS.gene067718.t1
chr4.2 exon 48963064 48963147 48963064 ID=MS.gene067718.t1.exon2;Parent=MS.gene067718.t1
chr4.2 CDS 48963064 48963147 48963064 ID=cds.MS.gene067718.t1;Parent=MS.gene067718.t1
chr4.2 exon 48964071 48964218 48964071 ID=MS.gene067718.t1.exon3;Parent=MS.gene067718.t1
chr4.2 CDS 48964071 48964218 48964071 ID=cds.MS.gene067718.t1;Parent=MS.gene067718.t1
chr4.2 exon 48964408 48964784 48964408 ID=MS.gene067718.t1.exon4;Parent=MS.gene067718.t1
chr4.2 CDS 48964408 48964784 48964408 ID=cds.MS.gene067718.t1;Parent=MS.gene067718.t1
Gene Sequence

>MS.gene067718

ATGGCTATTCTTCAAGGCGAGCTTGAACGAAAGAAAATGGAGAACTATAGATTAAAATTGATGTATGATCAGCTTAGAACAGATTACAATAACATGCAGATGTGTTTCGAGAAAGTGATGCTGGACCATAAGTTTAAGGAAGTTAAAGGAAAAGAAGTCTTTGATGGAAAATTTAAAGAGAAAGAGAGAACTGAGAATGGTGGAGTACTGGGACCGAGGAAAGATCTTGGATCGACTACTAATATGGTTAAGGAAGTTAAAGGACAAGAAATTTCTGAGGGGAAATTTGGACAGAAAAAGAGAATGAAGAATGGTGGAGAATTAATGAAAAGGAAATTTATTGATGCTGGATTGACTACTAATAAGGTTAAGGAAGTTAAAGGAAAAGAAGTTTTTAATGGAAAATGTGAAAAGAAAAAGAGACTTGAGAATGGTGAAGAGTTGGTGTCAAAGAAATGCAAAGATCTTGTGTTGCTTACTAATGTTGAGACTACGATGGATCATGAAGCTTGTTCCTCATCAATGAGAAAAGCTAGAAGTCAGGACCAGTTAGGATCGGCGATGAAAAGCATCGAGGTTTCTTCGAAGGAGTTAGTCCTTAGTAAGAATGTGGATGTTAATGTTGATAATGTTGAGGCAACATTGGTGAAAGCAAGAGTCTCTATTAGAGCTAGATCAGAAGAAACCATGGTATGTTAAAGTGTACATTAACTTTCAACATTGTAAGTTTTTATATGATAACTTGTTGTCCTTTTGGCTTAAAGAATTTATTTTTATTGTGTTAGATCACTAATGGATGTCAATGGAGAAAGTATGGACAAAAACTGTCGAAAGGAAACCCATGTCCTAAATCTTATTATCGAAAACAGGTAACTTTCTTCTATAATTATCGAAATTACATGTACTCCCTCCGTTTCAAAATACATGTCGCAATTTTACAATGTGCACTATTCACATAGCTTACTTTGACCATATTTTTTTTAACTAATATATAAATACAAATAATAACATGTAAGATGTTTTTTTATTTGTTTTGACGAATATTTTCAAAATATCAAATTTTTATAATTTTCTAGTATATAATTAAAGATATTAATGGTCAAAGTTGTGTATTGACATGTGTGCAGTGGTTGACTGCGACATGTATTTTGAAACGTAGGGAGTATTTATTAAGGTTTTAAATTTATGTCACCCTAGTGATTGCTTTCACATTATTATATTTAATATTGTACATAATTACAGTCAAATATGATTTATACAACGTCAACTGCATTAAATTTGCAACGACATTAAACTCTCCGAATGTTTCCTAGATCACTGTCAGAAACATGCATATTTTAAAACCTTGTTATTTAATCATTAGTTTATGTATATTTTCAATTAATTACAAAATAACTCTACATACATAAACATTATTAGTGATTATTTATTTGATATGCATTGCACGAGGTTGGATAACAATATAATTTAATTCAAATGTGTTTCCATTTTCAAAGCTCATGTTTCTATGCTAGAAAATGGATTTGGATTTGCTCCATTTCACAATCCATGCATTTACGCATCCATCACGTTGATGGCCATTAAAAATCAAAATATGCATTGAAGTTTGGATCATCCAATCTTCGATCCAACTACCATCGACGTCGACATACTTACTACGTGAATGCCTGGATTCGCTGAATGCAACAAACTCAAATCGAAAATGGTAGCTGCTAGCTTGGTTAATTAAAATACTTATGAAATGGTATGTACTGCAATGACAATATTATTTAATACAATTCAAATTTAACAGGTACAAAGGTGTGCTCTTGACAAAACAGTCGTAATCACAACTTATGAAGGAAATGACAGCCACCCTTTACCTACAGCAGCCAAAGAAATGGCAAAAACAACTTCAGCAGTTGCAAAGATGCTTCTTTCAGCATCAACATCAAGCAATGGTGGACAATTGAATAAAAATTTACCCACAAGAGCACTCCTTCCTTGTTCTTCAAGTATTTCAACTATCTCAGCTCCTGCGTCGTTTCCAACTATTACACTTGATTATACTCAATCTTCAAATACTCCACAAAGGAACCCAGATCAGTTCCAAACTCCTTTAATCTCTTAGAGTTCTGCAAATTCCTCAGCTTCTTTGATCCCTCAAATACCTAATCAAAACCAATCAAAATTTTCTGGACTACAAATGTCAAAATATGCAGATGGCCCTTCACAATTATTAGTAGTACCAAATATTGCTCAGGCAGTTAATGCTGCCATTGCTGCTAATCCAAATTTTCTTGCATCTTTGTCACCTGGTCTTACTTCCTTTATTGGTCATACTCAGCCAAACAACAACATTGCTTTTATTGATAGTAACAATGGCAACAATGTCACCGAAGGAAATCACAATGATAGCAACAATAATGTCACAGAAGGCAATAACAATGGAAATATCACAGGTAGCAACAATAATGTCACAGAAGGCAATAACAATGTCAAAATCTTTTTTGGCTCAAAGATTTAG
Protein sequence

>MS.gene067718.t1

MAILQGELERKKMENYRLKLMYDQLRTDYNNMQMCFEKVMLDHKFKEVKGKEVFDGKFKEKERTENGGVLGPRKDLGSTTNMVKEVKGQEISEGKFGQKKRMKNGGELMKRKFIDAGLTTNKVKEVKGKEVFNGKCEKKKRLENGEELVSKKCKDLVLLTNVETTMDHEACSSSMRKARSQDQLGSAMKSIEVSSKELVLSKNVDVNVDNVEATLVKARVSIRARSEETMITNGCQWRKYGQKLSKGNPCPKSYYRKQVQRCALDKTVVITTYEGNDSHPLPTAAKEMAKTTSAVAKMLLSASTSSNASLIPQIPNQNQSKFSGLQMSKYADGPSQLLVVPNIAQAVNAAIAANPNFLASLSPGLTSFIGHTQPNNNIAFIDSNNGNNVTEGNHNDSNNNVTEGNNNGNITGSNNNVTEGNNNVKIFFGSKI