Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068099.t1 | RHN47673.1 | 89.6 | 230 | 21 | 1 | 1 | 227 | 5 | 234 | 3.70E-108 | 401 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068099.t1 | Q39261 | 38.6 | 88 | 41 | 4 | 74 | 158 | 50 | 127 | 5.4e-07 | 56.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068099.t1 | A0A396H4J8 | 89.6 | 230 | 21 | 1 | 1 | 227 | 5 | 234 | 2.7e-108 | 401.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene068099.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068099.t1 | MTR_7g022250 | 80.087 | 231 | 42 | 2 | 1 | 227 | 9 | 239 | 8.19e-137 | 384 |
| MS.gene068099.t1 | MTR_7g028890 | 78.261 | 230 | 33 | 2 | 1 | 227 | 5 | 220 | 1.61e-126 | 357 |
| MS.gene068099.t1 | MTR_7g028915 | 75.217 | 230 | 32 | 2 | 1 | 227 | 5 | 212 | 4.00e-120 | 340 |
| MS.gene068099.t1 | MTR_7g022270 | 59.375 | 192 | 40 | 4 | 18 | 207 | 6 | 161 | 2.35e-71 | 215 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068099.t1 | AT5G57520 | 33.051 | 118 | 57 | 6 | 74 | 186 | 50 | 150 | 9.40e-11 | 58.5 |
Find 34 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTGTGGATATCAATATTA+TGG | 0.285744 | 7.3:+20705559 | MS.gene068099:CDS |
| AAGAAATGGAGAAATTTCTT+TGG | 0.320928 | 7.3:-20705311 | None:intergenic |
| TATATATATATTAAAGCTTA+AGG | 0.335043 | 7.3:-20705825 | None:intergenic |
| TCCAAGCATTGTTAAAAGCT+TGG | 0.375606 | 7.3:-20705477 | None:intergenic |
| CTTAAGGGTCAAATCAATCT+TGG | 0.381650 | 7.3:-20705809 | None:intergenic |
| CACCATCTCTCCATTCCATA+TGG | 0.384400 | 7.3:+20705537 | MS.gene068099:CDS |
| ATTGATATCCACACCCCATA+TGG | 0.402297 | 7.3:-20705552 | None:intergenic |
| AATTACTCAAATGGTGATCA+AGG | 0.411277 | 7.3:+20705502 | MS.gene068099:CDS |
| AATATGTTTGAGATTGAAGT+TGG | 0.424994 | 7.3:+20705712 | MS.gene068099:CDS |
| AACAATATGATTCAGCATGC+AGG | 0.425567 | 7.3:+20705586 | MS.gene068099:CDS |
| ATGTTGAAACTCAGAATTCA+AGG | 0.446337 | 7.3:+20705245 | MS.gene068099:CDS |
| GCTTGGATAAATTACTCAAA+TGG | 0.450589 | 7.3:+20705493 | MS.gene068099:CDS |
| AGTAGCCAATTTGAAGATGA+TGG | 0.452167 | 7.3:+20705180 | MS.gene068099:CDS |
| TTCACTAGTCCACAAGCATT+AGG | 0.461133 | 7.3:+20705406 | MS.gene068099:CDS |
| TCACAGGAAAAGAATTCAAA+AGG | 0.463105 | 7.3:+20705354 | MS.gene068099:CDS |
| TCATCTGCTTCATCTATCTC+TGG | 0.464587 | 7.3:-20705658 | None:intergenic |
| ACCATCTCTCCATTCCATAT+GGG | 0.464694 | 7.3:+20705538 | MS.gene068099:CDS |
| TAGAGAGAAGTCTCAAGAAA+AGG | 0.476425 | 7.3:+20705452 | MS.gene068099:CDS |
| TTGGATTCATTGTTGATCAC+AGG | 0.479894 | 7.3:+20705338 | MS.gene068099:CDS |
| ATATATATATTAAAGCTTAA+GGG | 0.490897 | 7.3:-20705824 | None:intergenic |
| TGTTAGTGATGAAGTTGATC+AGG | 0.506770 | 7.3:+20705756 | MS.gene068099:CDS |
| TCTCCATTCCATATGGGGTG+TGG | 0.536840 | 7.3:+20705544 | MS.gene068099:CDS |
| GGAAAATCAGAACAAGAGAA+AGG | 0.539450 | 7.3:+20705201 | MS.gene068099:CDS |
| CAAGAGAAAGGCAAATGTGA+TGG | 0.544866 | 7.3:+20705213 | MS.gene068099:CDS |
| TGATGACCACCTAATGCTTG+TGG | 0.552879 | 7.3:-20705415 | None:intergenic |
| ACAATATGATTCAGCATGCA+GGG | 0.561522 | 7.3:+20705587 | MS.gene068099:CDS |
| CCCCATATGGAATGGAGAGA+TGG | 0.561790 | 7.3:-20705539 | None:intergenic |
| GTGCAACAAGGGGAAGGAGA+AGG | 0.585340 | 7.3:+20705144 | None:intergenic |
| TATCCACACCCCATATGGAA+TGG | 0.594771 | 7.3:-20705547 | None:intergenic |
| CCATCTCTCCATTCCATATG+GGG | 0.598148 | 7.3:+20705539 | MS.gene068099:CDS |
| ATTACTCAAATGGTGATCAA+GGG | 0.605537 | 7.3:+20705503 | MS.gene068099:CDS |
| GGAAGACAAAGAGACTCAAG+AGG | 0.634668 | 7.3:+20705777 | MS.gene068099:CDS |
| AGACAAAGAGACTCAAGAGG+AGG | 0.673262 | 7.3:+20705780 | MS.gene068099:CDS |
| ACTAGTCCACAAGCATTAGG+TGG | 0.698124 | 7.3:+20705409 | MS.gene068099:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAAATGGAGAAATTTCTT+TGG | - | chr7.3:20705314-20705333 | None:intergenic | 25.0% |
| ! | ATGAATCCAAAAAGAAGAAA+TGG | - | chr7.3:20705328-20705347 | None:intergenic | 25.0% |
| !! | AATATGTTTGAGATTGAAGT+TGG | + | chr7.3:20705712-20705731 | MS.gene068099:CDS | 25.0% |
| !! | ATTTCTCCATTTCTTCTTTT+TGG | + | chr7.3:20705319-20705338 | MS.gene068099:CDS | 25.0% |
| !! | ATTTTCCATCATCTTCAAAT+TGG | - | chr7.3:20705188-20705207 | None:intergenic | 25.0% |
| !! | TTCTTCAACAACATCTTTTT+GGG | - | chr7.3:20705275-20705294 | None:intergenic | 25.0% |
| AATTACTCAAATGGTGATCA+AGG | + | chr7.3:20705502-20705521 | MS.gene068099:CDS | 30.0% | |
| ATGTTGAAACTCAGAATTCA+AGG | + | chr7.3:20705245-20705264 | MS.gene068099:CDS | 30.0% | |
| ATTACTCAAATGGTGATCAA+GGG | + | chr7.3:20705503-20705522 | MS.gene068099:CDS | 30.0% | |
| GCTTGGATAAATTACTCAAA+TGG | + | chr7.3:20705493-20705512 | MS.gene068099:CDS | 30.0% | |
| TCACAGGAAAAGAATTCAAA+AGG | + | chr7.3:20705354-20705373 | MS.gene068099:CDS | 30.0% | |
| ! | ATTTGACCCTTAAGCTTTAA+GGG | + | chr7.3:20705818-20705837 | MS.gene068099:CDS | 30.0% |
| ! | CTTCTTCAACAACATCTTTT+TGG | - | chr7.3:20705276-20705295 | None:intergenic | 30.0% |
| ! | TCTTCAACAACATCTTTTTG+GGG | - | chr7.3:20705274-20705293 | None:intergenic | 30.0% |
| ! | TTTTATGCAGGGAATTTGTA+TGG | + | chr7.3:20705619-20705638 | MS.gene068099:CDS | 30.0% |
| AACAATATGATTCAGCATGC+AGG | + | chr7.3:20705586-20705605 | MS.gene068099:CDS | 35.0% | |
| ACAATATGATTCAGCATGCA+GGG | + | chr7.3:20705587-20705606 | MS.gene068099:CDS | 35.0% | |
| AGTAGCCAATTTGAAGATGA+TGG | + | chr7.3:20705180-20705199 | MS.gene068099:CDS | 35.0% | |
| CTTAAGGGTCAAATCAATCT+TGG | - | chr7.3:20705812-20705831 | None:intergenic | 35.0% | |
| GATTTGACCCTTAAGCTTTA+AGG | + | chr7.3:20705817-20705836 | MS.gene068099:CDS | 35.0% | |
| GGAAAATCAGAACAAGAGAA+AGG | + | chr7.3:20705201-20705220 | MS.gene068099:CDS | 35.0% | |
| GGGTGTGGATATCAATATTA+TGG | + | chr7.3:20705559-20705578 | MS.gene068099:CDS | 35.0% | |
| TAGAGAGAAGTCTCAAGAAA+AGG | + | chr7.3:20705452-20705471 | MS.gene068099:CDS | 35.0% | |
| TTGGATTCATTGTTGATCAC+AGG | + | chr7.3:20705338-20705357 | MS.gene068099:CDS | 35.0% | |
| ! | TCCAAGCATTGTTAAAAGCT+TGG | - | chr7.3:20705480-20705499 | None:intergenic | 35.0% |
| ! | TCCAAGCTTTTAACAATGCT+TGG | + | chr7.3:20705476-20705495 | MS.gene068099:CDS | 35.0% |
| ! | TGTTAGTGATGAAGTTGATC+AGG | + | chr7.3:20705756-20705775 | MS.gene068099:CDS | 35.0% |
| !! | TGAAGTTGGTTCAGTTTTAG+AGG | + | chr7.3:20705726-20705745 | MS.gene068099:CDS | 35.0% |
| !!! | GAAGTTGGTTCAGTTTTAGA+GGG | + | chr7.3:20705727-20705746 | MS.gene068099:CDS | 35.0% |
| ACCATCTCTCCATTCCATAT+GGG | + | chr7.3:20705538-20705557 | MS.gene068099:CDS | 40.0% | |
| ATTGATATCCACACCCCATA+TGG | - | chr7.3:20705555-20705574 | None:intergenic | 40.0% | |
| CAAGAGAAAGGCAAATGTGA+TGG | + | chr7.3:20705213-20705232 | MS.gene068099:CDS | 40.0% | |
| TCATCTGCTTCATCTATCTC+TGG | - | chr7.3:20705661-20705680 | None:intergenic | 40.0% | |
| TTCACTAGTCCACAAGCATT+AGG | + | chr7.3:20705406-20705425 | MS.gene068099:CDS | 40.0% | |
| ! | GGTCATCGAACTTTTATGCA+GGG | + | chr7.3:20705608-20705627 | MS.gene068099:CDS | 40.0% |
| ACTAGTCCACAAGCATTAGG+TGG | + | chr7.3:20705409-20705428 | MS.gene068099:CDS | 45.0% | |
| AGACAAAGAGACTCAAGAGG+AGG | + | chr7.3:20705780-20705799 | MS.gene068099:CDS | 45.0% | |
| CACCATCTCTCCATTCCATA+TGG | + | chr7.3:20705537-20705556 | MS.gene068099:CDS | 45.0% | |
| CCATCTCTCCATTCCATATG+GGG | + | chr7.3:20705539-20705558 | MS.gene068099:CDS | 45.0% | |
| GGAAGACAAAGAGACTCAAG+AGG | + | chr7.3:20705777-20705796 | MS.gene068099:CDS | 45.0% | |
| TATCCACACCCCATATGGAA+TGG | - | chr7.3:20705550-20705569 | None:intergenic | 45.0% | |
| TGATGACCACCTAATGCTTG+TGG | - | chr7.3:20705418-20705437 | None:intergenic | 45.0% | |
| ! | GGGTCATCGAACTTTTATGC+AGG | + | chr7.3:20705607-20705626 | MS.gene068099:CDS | 45.0% |
| !!! | GTTGGTTCAGTTTTAGAGGG+AGG | + | chr7.3:20705730-20705749 | MS.gene068099:CDS | 45.0% |
| CCCCATATGGAATGGAGAGA+TGG | - | chr7.3:20705542-20705561 | None:intergenic | 50.0% | |
| TCTCCATTCCATATGGGGTG+TGG | + | chr7.3:20705544-20705563 | MS.gene068099:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 20705154 | 20705837 | 20705154 | ID=MS.gene068099 |
| chr7.3 | mRNA | 20705154 | 20705837 | 20705154 | ID=MS.gene068099.t1;Parent=MS.gene068099 |
| chr7.3 | exon | 20705154 | 20705837 | 20705154 | ID=MS.gene068099.t1.exon1;Parent=MS.gene068099.t1 |
| chr7.3 | CDS | 20705154 | 20705837 | 20705154 | ID=cds.MS.gene068099.t1;Parent=MS.gene068099.t1 |
| Gene Sequence |
| Protein sequence |
