AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068201

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068201.t1 MTR_1g050502 79.638 221 29 2 1 221 1 205 5.56e-122 345
MS.gene068201.t1 MTR_1g050502 87.113 194 10 1 1 194 1 179 1.35e-119 342
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068201.t1 AT1G42990 51.562 64 31 0 135 198 155 218 2.12e-13 68.6

Find 68 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTCCCTGCTATGGTTCC+TGG 0.201475 1.4:+36649977 MS.gene068201:CDS
GAGAGATTCGAAATCGATTT+GGG 0.252626 1.4:-36648000 None:intergenic
CTAAGAAGGGTGGCTCTAAA+AGG 0.288472 1.4:+36650083 MS.gene068201:CDS
AGAGAGATTCGAAATCGATT+TGG 0.334822 1.4:-36648001 None:intergenic
GAAGGCTCTTGAGGAGTTAC+TGG 0.356888 1.4:-36648263 None:intergenic
GGAACCTCTTGAGGAGTTAC+TGG 0.381189 1.4:-36648308 None:intergenic
TTGCAATCGCGTGGTGGCTT+TGG 0.414223 1.4:+36648694 MS.gene068201:CDS
TGCTGTTGGGTTCCCTGCTA+TGG 0.415075 1.4:+36649970 MS.gene068201:CDS
TTTGCAGAATCCCTGCTGTT+GGG 0.426943 1.4:+36649957 MS.gene068201:intron
GAATATCTTCGAGAAACTTA+TGG 0.427802 1.4:-36648175 None:intergenic
CCTCGACCCGATCCCTCAAT+CGG 0.430109 1.4:+36648199 MS.gene068201:CDS
GGTTCCCTGCTATGGTTCCT+GGG 0.440801 1.4:+36649978 MS.gene068201:CDS
AGCCTTCTTCCGATGAAGCC+AGG 0.446524 1.4:+36648279 MS.gene068201:CDS
TGGATCAACCCTGGCTTCAT+CGG 0.451066 1.4:-36648288 None:intergenic
TGATCATCCGATTGAGGGAT+CGG 0.459847 1.4:-36648206 None:intergenic
TGATGCCCAGGAACCATAGC+AGG 0.462353 1.4:-36649983 None:intergenic
TGAAGGGGAATGCAGAAGAC+TGG 0.491226 1.4:+36648621 MS.gene068201:CDS
TGGAATTGCGTCGTCATCAT+CGG 0.494775 1.4:+36648142 MS.gene068201:CDS
AGCAGACTCCTGCATGGTCA+TGG 0.499805 1.4:-36648722 None:intergenic
AACCTTGATCATCCGATTGA+GGG 0.507174 1.4:-36648211 None:intergenic
ATGCTGAGAACCATGCTTTG+CGG 0.510527 1.4:+36648665 MS.gene068201:CDS
AAACCTTGATCATCCGATTG+AGG 0.512112 1.4:-36648212 None:intergenic
CCGATTGAGGGATCGGGTCG+AGG 0.513783 1.4:-36648199 None:intergenic
CTGTTGTTAGCCACAGTGGA+AGG 0.514860 1.4:-36650025 None:intergenic
TGGAACACAAAGGTCTAAGA+AGG 0.519321 1.4:+36650069 MS.gene068201:CDS
TGGTGCTCCCATGACCATGC+AGG 0.523079 1.4:+36648714 MS.gene068201:CDS
ACCCTGGCTTCATCGGAAGA+AGG 0.528508 1.4:-36648281 None:intergenic
TTGGGGTTCTAAATTGTCCA+TGG 0.534572 1.4:-36647982 None:intergenic
TCATCGGAAGAAGGCTCTTG+AGG 0.542473 1.4:-36648272 None:intergenic
AGGAGTTACTGGATCAACTC+TGG 0.544068 1.4:-36648252 None:intergenic
TTCGAGAAACTTATGGTAGT+CGG 0.545198 1.4:-36648168 None:intergenic
GTTGTTAGCCACAGTGGAAG+GGG 0.545341 1.4:-36650023 None:intergenic
ATCCCTCAATCGGATGATCA+AGG 0.545906 1.4:+36648209 MS.gene068201:CDS
TCAACTCTGGCTTCATCAGA+AGG 0.547608 1.4:-36648239 None:intergenic
ATAGCAGGGAACCCAACAGC+AGG 0.548068 1.4:-36649968 None:intergenic
CTTTGTTTGCAATCGCGTGG+TGG 0.550397 1.4:+36648688 MS.gene068201:CDS
TAAGAAGGGTGGCTCTAAAA+GGG 0.555483 1.4:+36650084 MS.gene068201:CDS
GCCTTCTTCCGATGAAGCCA+GGG 0.556367 1.4:+36648280 MS.gene068201:CDS
GCAGACTCCTGCATGGTCAT+GGG 0.561161 1.4:-36648721 None:intergenic
CTTCTCATGTCTGATGCCGA+TGG 0.561993 1.4:+36648122 MS.gene068201:CDS
GCAAACAAAGCCGCAAAGCA+TGG 0.573484 1.4:-36648675 None:intergenic
TGAGTTGGCACATGATGCCC+AGG 0.575121 1.4:-36649995 None:intergenic
TCAATCTCTGAGAGAGGCGA+GGG 0.577892 1.4:-36648095 None:intergenic
GATCATCCGATTGAGGGATC+GGG 0.580413 1.4:-36648205 None:intergenic
TGTTGTTAGCCACAGTGGAA+GGG 0.582265 1.4:-36650024 None:intergenic
CTCAATCTCTGAGAGAGGCG+AGG 0.584965 1.4:-36648096 None:intergenic
GGTTCAGGAGGAACCTCTTG+AGG 0.589392 1.4:-36648317 None:intergenic
TGATGACGACGCAATTCCAT+CGG 0.590462 1.4:-36648138 None:intergenic
GTTACCTCCAAGAGAAAACA+TGG 0.596124 1.4:+36650049 MS.gene068201:CDS
CTGCTGTGAAGTCAAGAGAG+AGG 0.599823 1.4:+36648554 MS.gene068201:CDS
GTGGAAGGGGTAGACTGAGT+TGG 0.606292 1.4:-36650010 None:intergenic
AGGAGTTACTGGATCAACCC+TGG 0.607536 1.4:-36648297 None:intergenic
CGGCTTTGTTTGCAATCGCG+TGG 0.608761 1.4:+36648685 MS.gene068201:CDS
GTGTCTGCGACGGAAACAGA+AGG 0.621465 1.4:-36648071 None:intergenic
AACACTGTTGTTAGCCACAG+TGG 0.628137 1.4:-36650029 None:intergenic
AAAATCTTGAGACGAAGAGT+AGG 0.630085 1.4:+36648593 MS.gene068201:CDS
AGGCGAGGGAGTGTCTGCGA+CGG 0.634385 1.4:-36648081 None:intergenic
TGATCCAGTAACTCCTCAAG+AGG 0.636811 1.4:+36648304 MS.gene068201:CDS
AGATTCTCAATCTCTGAGAG+AGG 0.640144 1.4:-36648101 None:intergenic
AGAGATTCGAAATCGATTTG+GGG 0.646761 1.4:-36647999 None:intergenic
TTTCAGGCAGATGAGGAACA+GGG 0.660559 1.4:+36648528 MS.gene068201:intron
GGAACACAAAGGTCTAAGAA+GGG 0.664648 1.4:+36650070 MS.gene068201:CDS
TAGCAGGGAACCCAACAGCA+GGG 0.674308 1.4:-36649967 None:intergenic
ACACAAAGGTCTAAGAAGGG+TGG 0.678689 1.4:+36650073 MS.gene068201:CDS
GAGCACAGCAGACTCCTGCA+TGG 0.688063 1.4:-36648728 None:intergenic
GATGCCCAGGAACCATAGCA+GGG 0.695327 1.4:-36649982 None:intergenic
AGAGAAAACATGGAACACAA+AGG 0.721160 1.4:+36650059 MS.gene068201:CDS
TCAGTCTACCCCTTCCACTG+TGG 0.742334 1.4:+36650015 MS.gene068201:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTTTCAATTTCATATAT+TGG + chr1.4:36648987-36649006 MS.gene068201:intron 15.0%
!!! TGTCATTATCATTTTATTTT+AGG + chr1.4:36648810-36648829 MS.gene068201:intron 15.0%
!! ATACATAAACTCACTAATTA+TGG - chr1.4:36649479-36649498 None:intergenic 20.0%
!! ATTCATTTAACATGCATAAA+GGG - chr1.4:36649811-36649830 None:intergenic 20.0%
!! GTAAGGAAAAAAAAATTGTT+AGG - chr1.4:36648933-36648952 None:intergenic 20.0%
!! TAAATGGAAAAAAATTGTGT+GGG + chr1.4:36649593-36649612 MS.gene068201:intron 20.0%
!! TACATAAAACATAAACAACA+TGG + chr1.4:36649753-36649772 MS.gene068201:intron 20.0%
!! TATTCATTTAACATGCATAA+AGG - chr1.4:36649812-36649831 None:intergenic 20.0%
!!! TAATTTGTAATTTGTATTCG+AGG + chr1.4:36648439-36648458 MS.gene068201:intron 20.0%
! AACTATATAGCATTAGCAAA+AGG - chr1.4:36648973-36648992 None:intergenic 25.0%
! AGAGATTAAGGTTACAATTT+GGG - chr1.4:36649027-36649046 None:intergenic 25.0%
! CTAAATGGAAAAAAATTGTG+TGG + chr1.4:36649592-36649611 MS.gene068201:intron 25.0%
! TAGAGATTAAGGTTACAATT+TGG - chr1.4:36649028-36649047 None:intergenic 25.0%
! TTTAACAGTGTTATGAATAG+TGG + chr1.4:36649175-36649194 MS.gene068201:intron 25.0%
!! AAACAGAAAACCTTAGATTT+TGG + chr1.4:36649348-36649367 MS.gene068201:intron 25.0%
!! CTAAAAGTATAACCATATGA+AGG + chr1.4:36648849-36648868 MS.gene068201:intron 25.0%
!! TTTTTCCTTACTTTCTTGAA+TGG + chr1.4:36648942-36648961 MS.gene068201:intron 25.0%
!!! ACGTCTTTAGGTTTTTTATT+GGG + chr1.4:36649113-36649132 MS.gene068201:intron 25.0%
!!! CCTAATTAGCTTTTTTGAAA+CGG - chr1.4:36648342-36648361 None:intergenic 25.0%
!!! CTAATTAGCTTTTTTGAAAC+GGG - chr1.4:36648341-36648360 None:intergenic 25.0%
!!! TTTTTTTTTTTTCAACCTGC+TGG + chr1.4:36648414-36648433 MS.gene068201:intron 25.0%
AAATTACAAATTACTCCAGC+AGG - chr1.4:36648432-36648451 None:intergenic 30.0%
ACAGTAGTCATTAGAGATTA+AGG - chr1.4:36649039-36649058 None:intergenic 30.0%
ACCTTTGGACCAAAAAAAAA+AGG - chr1.4:36649689-36649708 None:intergenic 30.0%
ATAACAGCAAGAAGAAAACA+TGG - chr1.4:36649075-36649094 None:intergenic 30.0%
ATAATATTTCACCTTCTCAC+TGG + chr1.4:36649446-36649465 MS.gene068201:intron 30.0%
CCGTTTCAAAAAAGCTAATT+AGG + chr1.4:36648339-36648358 MS.gene068201:CDS 30.0%
CTAAGATACTCCAAAATCTA+AGG - chr1.4:36649361-36649380 None:intergenic 30.0%
GAATATCTTCGAGAAACTTA+TGG - chr1.4:36648178-36648197 None:intergenic 30.0%
GGTTTCATTATTGCATTCTT+TGG + chr1.4:36648899-36648918 MS.gene068201:intron 30.0%
TAGTGAGTTTATGTATGTAC+TGG + chr1.4:36649483-36649502 MS.gene068201:intron 30.0%
TATTGCATTCTTTGGTTGTT+TGG + chr1.4:36648907-36648926 MS.gene068201:intron 30.0%
TTTGCATAAGTACACAAATC+TGG - chr1.4:36649732-36649751 None:intergenic 30.0%
! GTTTTATGAAGTTGCTCTAA+TGG + chr1.4:36649883-36649902 MS.gene068201:intron 30.0%
!! ATCTAAGGTTTTCTGTTTCA+TGG - chr1.4:36649346-36649365 None:intergenic 30.0%
!! TCTAAGGTTTTCTGTTTCAT+GGG - chr1.4:36649345-36649364 None:intergenic 30.0%
!! TTTAGGTTTTTGCGTCTAAA+TGG + chr1.4:36649577-36649596 MS.gene068201:intron 30.0%
!!! CTTAGATTTTGGAGTATCTT+AGG + chr1.4:36649359-36649378 MS.gene068201:intron 30.0%
!!! GACGTCTTTAGGTTTTTTAT+TGG + chr1.4:36649112-36649131 MS.gene068201:intron 30.0%
!!! TACTTTTTCTCTGAGAGTTT+TGG - chr1.4:36649784-36649803 None:intergenic 30.0%
!!! TGTTTTGGTATCTGATTTTC+AGG + chr1.4:36648512-36648531 MS.gene068201:intron 30.0%
AAAAACACCATGACAGTGTT+CGG + chr1.4:36649397-36649416 MS.gene068201:intron 35.0%
AAAATCTTGAGACGAAGAGT+AGG + chr1.4:36648593-36648612 MS.gene068201:CDS 35.0%
AGAGAAAACATGGAACACAA+AGG + chr1.4:36650059-36650078 MS.gene068201:CDS 35.0%
AGAGAGATTCGAAATCGATT+TGG - chr1.4:36648004-36648023 None:intergenic 35.0%
AGCAACTATGCTATGTTATC+TGG + chr1.4:36649533-36649552 MS.gene068201:intron 35.0%
ATCATCCTCCTGCAAAAAAT+CGG - chr1.4:36648045-36648064 None:intergenic 35.0%
CAAAAAAAAAAGGCTACAGG+AGG - chr1.4:36649679-36649698 None:intergenic 35.0%
CAAATCTGGATTACAGCAAA+AGG - chr1.4:36649718-36649737 None:intergenic 35.0%
CCTAAAGACGTCTTTAACAT+CGG - chr1.4:36649104-36649123 None:intergenic 35.0%
GACCAAAAAAAAAAGGCTAC+AGG - chr1.4:36649682-36649701 None:intergenic 35.0%
GCAACTATGCTATGTTATCT+GGG + chr1.4:36649534-36649553 MS.gene068201:intron 35.0%
TAACAATCACAACCACTTGT+TGG + chr1.4:36649631-36649650 MS.gene068201:intron 35.0%
TCCATCCATTCAAGAAAGTA+AGG - chr1.4:36648950-36648969 None:intergenic 35.0%
TCCTTACTTTCTTGAATGGA+TGG + chr1.4:36648946-36648965 MS.gene068201:intron 35.0%
TGACCTCAAAAACACTATAG+CGG - chr1.4:36649514-36649533 None:intergenic 35.0%
TGTTATGAATAGTGGTGTAG+CGG + chr1.4:36649183-36649202 MS.gene068201:intron 35.0%
TTCGAGAAACTTATGGTAGT+CGG - chr1.4:36648171-36648190 None:intergenic 35.0%
! GAGAGATTCGAAATCGATTT+GGG - chr1.4:36648003-36648022 None:intergenic 35.0%
! TTGTGTTCCATGTTTTCTCT+TGG - chr1.4:36650059-36650078 None:intergenic 35.0%
!! ACGAAGAGTAGGTATTTTGA+AGG + chr1.4:36648604-36648623 MS.gene068201:CDS 35.0%
!! AGAGATTCGAAATCGATTTG+GGG - chr1.4:36648002-36648021 None:intergenic 35.0%
!! CGAAGAGTAGGTATTTTGAA+GGG + chr1.4:36648605-36648624 MS.gene068201:CDS 35.0%
!! GAAGAGTAGGTATTTTGAAG+GGG + chr1.4:36648606-36648625 MS.gene068201:CDS 35.0%
!!! CTCCTGTAGCCTTTTTTTTT+TGG + chr1.4:36649677-36649696 MS.gene068201:intron 35.0%
!!! GCCTTTTTTTTTTGGTCCAA+AGG + chr1.4:36649685-36649704 MS.gene068201:intron 35.0%
!!! GGTTCTAATGAGAAGTGTTT+TGG + chr1.4:36648497-36648516 MS.gene068201:intron 35.0%
AAAACACCATGACAGTGTTC+GGG + chr1.4:36649398-36649417 MS.gene068201:intron 40.0%
AAAACCCACTCTTCGAATGA+TGG - chr1.4:36649868-36649887 None:intergenic 40.0%
AAACCTTGATCATCCGATTG+AGG - chr1.4:36648215-36648234 None:intergenic 40.0%
AACCTTGATCATCCGATTGA+GGG - chr1.4:36648214-36648233 None:intergenic 40.0%
ATCTGGATTACAGCAAAAGG+AGG - chr1.4:36649715-36649734 None:intergenic 40.0%
ATTTCAGATCTCATCTCGTG+CGG - chr1.4:36649293-36649312 None:intergenic 40.0%
CCATAAGACCCCATCAAATT+CGG - chr1.4:36648468-36648487 None:intergenic 40.0%
CCGATGTTAAAGACGTCTTT+AGG + chr1.4:36649101-36649120 MS.gene068201:intron 40.0%
GAAACCAAATCATTCGTGCA+AGG - chr1.4:36648885-36648904 None:intergenic 40.0%
GACCTCAAAAACACTATAGC+GGG - chr1.4:36649513-36649532 None:intergenic 40.0%
GGAACACAAAGGTCTAAGAA+GGG + chr1.4:36650070-36650089 MS.gene068201:CDS 40.0%
GGTCACTGTAATCCTTCATA+TGG - chr1.4:36648864-36648883 None:intergenic 40.0%
GTTACCTCCAAGAGAAAACA+TGG + chr1.4:36650049-36650068 MS.gene068201:CDS 40.0%
TCGAGGTTACCGAATTTGAT+GGG + chr1.4:36648456-36648475 MS.gene068201:intron 40.0%
TCTTCGAATGATGGCAACAT+TGG - chr1.4:36649859-36649878 None:intergenic 40.0%
TGGAACACAAAGGTCTAAGA+AGG + chr1.4:36650069-36650088 MS.gene068201:CDS 40.0%
TTCGAGGTTACCGAATTTGA+TGG + chr1.4:36648455-36648474 MS.gene068201:intron 40.0%
! AGATTCTCAATCTCTGAGAG+AGG - chr1.4:36648104-36648123 None:intergenic 40.0%
! CGAATTTGATGGGGTCTTAT+GGG + chr1.4:36648466-36648485 MS.gene068201:intron 40.0%
! GATACATACCACTGCTGTTT+AGG + chr1.4:36649560-36649579 MS.gene068201:intron 40.0%
! TGTTCCATGTTTTCTCTTGG+AGG - chr1.4:36650056-36650075 None:intergenic 40.0%
!! ATCCCGCTATAGTGTTTTTG+AGG + chr1.4:36649508-36649527 MS.gene068201:intron 40.0%
!! ATCTGATTTTCAGGCAGATG+AGG + chr1.4:36648521-36648540 MS.gene068201:intron 40.0%
!! TAAGAAGGGTGGCTCTAAAA+GGG + chr1.4:36650084-36650103 MS.gene068201:CDS 40.0%
!!! AGCTTTTTTGAAACGGGTTC+AGG - chr1.4:36648335-36648354 None:intergenic 40.0%
!!! TTTTTTGAAACGGGTTCAGG+AGG - chr1.4:36648332-36648351 None:intergenic 40.0%
AACACTGTTGTTAGCCACAG+TGG - chr1.4:36650032-36650051 None:intergenic 45.0%
ACACAAAGGTCTAAGAAGGG+TGG + chr1.4:36650073-36650092 MS.gene068201:CDS 45.0%
AGGAGTTACTGGATCAACTC+TGG - chr1.4:36648255-36648274 None:intergenic 45.0%
ATCCCTCAATCGGATGATCA+AGG + chr1.4:36648209-36648228 MS.gene068201:CDS 45.0%
ATGCTGAGAACCATGCTTTG+CGG + chr1.4:36648665-36648684 MS.gene068201:CDS 45.0%
CCGAATTTGATGGGGTCTTA+TGG + chr1.4:36648465-36648484 MS.gene068201:intron 45.0%
CGAGGTTACCGAATTTGATG+GGG + chr1.4:36648457-36648476 MS.gene068201:intron 45.0%
CGCAAAAACCTAAACAGCAG+TGG - chr1.4:36649571-36649590 None:intergenic 45.0%
CTCTGACAAAGACCAACAAG+TGG - chr1.4:36649646-36649665 None:intergenic 45.0%
CTTCTCATGCTGTCTCATTC+AGG + chr1.4:36649421-36649440 MS.gene068201:intron 45.0%
GGCCAATATAGCTCATGTTC+CGG + chr1.4:36649256-36649275 MS.gene068201:intron 45.0%
GTGACCTTGCACGAATGATT+TGG + chr1.4:36648878-36648897 MS.gene068201:intron 45.0%
GTTGCCATCATTCGAAGAGT+GGG + chr1.4:36649861-36649880 MS.gene068201:intron 45.0%
TGATCATCCGATTGAGGGAT+CGG - chr1.4:36648209-36648228 None:intergenic 45.0%
TGATCCAGTAACTCCTCAAG+AGG + chr1.4:36648259-36648278 MS.gene068201:CDS 45.0%
TGGAATTGCGTCGTCATCAT+CGG + chr1.4:36648142-36648161 MS.gene068201:CDS 45.0%
TGTTGCCATCATTCGAAGAG+TGG + chr1.4:36649860-36649879 MS.gene068201:intron 45.0%
TGTTGTTAGCCACAGTGGAA+GGG - chr1.4:36650027-36650046 None:intergenic 45.0%
TTTCAGGCAGATGAGGAACA+GGG + chr1.4:36648528-36648547 MS.gene068201:intron 45.0%
TTTGCAGAATCCCTGCTGTT+GGG + chr1.4:36649957-36649976 MS.gene068201:intron 45.0%
! AGCAAAAGGAGGTACACCTT+TGG - chr1.4:36649704-36649723 None:intergenic 45.0%
! AGCTTGTTTGTAGCAGCCAA+GGG - chr1.4:36649214-36649233 None:intergenic 45.0%
! GGGGTCTTATGGGATGATTA+TGG + chr1.4:36648476-36648495 MS.gene068201:intron 45.0%
! TCAACTCTGGCTTCATCAGA+AGG - chr1.4:36648242-36648261 None:intergenic 45.0%
! TGATGACGACGCAATTCCAT+CGG - chr1.4:36648141-36648160 None:intergenic 45.0%
! TTTTGCAGAATCCCTGCTGT+TGG + chr1.4:36649956-36649975 MS.gene068201:intron 45.0%
!! CTAAGAAGGGTGGCTCTAAA+AGG + chr1.4:36650083-36650102 MS.gene068201:CDS 45.0%
!! TTTTCAGGCAGATGAGGAAC+AGG + chr1.4:36648527-36648546 MS.gene068201:intron 45.0%
AGGAGTTACTGGATCAACCC+TGG - chr1.4:36648300-36648319 None:intergenic 50.0%
ATGGTGACTGACCAGTGAGA+AGG - chr1.4:36649460-36649479 None:intergenic 50.0%
CTGCTGTGAAGTCAAGAGAG+AGG + chr1.4:36648554-36648573 MS.gene068201:CDS 50.0%
CTGTTGTTAGCCACAGTGGA+AGG - chr1.4:36650028-36650047 None:intergenic 50.0%
CTTCTCATGTCTGATGCCGA+TGG + chr1.4:36648122-36648141 MS.gene068201:CDS 50.0%
GAAGGCTCTTGAGGAGTTAC+TGG - chr1.4:36648266-36648285 None:intergenic 50.0%
GAGTCTGCTGTGCTCTTGTT+GGG + chr1.4:36648736-36648755 MS.gene068201:intron 50.0%
GATCATCCGATTGAGGGATC+GGG - chr1.4:36648208-36648227 None:intergenic 50.0%
GCAAACAAAGCCGCAAAGCA+TGG - chr1.4:36648678-36648697 None:intergenic 50.0%
GGAACCTCTTGAGGAGTTAC+TGG - chr1.4:36648311-36648330 None:intergenic 50.0%
GGCCGGAACATGAGCTATAT+TGG - chr1.4:36649261-36649280 None:intergenic 50.0%
GTTGTTAGCCACAGTGGAAG+GGG - chr1.4:36650026-36650045 None:intergenic 50.0%
TCATCGGAAGAAGGCTCTTG+AGG - chr1.4:36648275-36648294 None:intergenic 50.0%
TGAAGGGGAATGCAGAAGAC+TGG + chr1.4:36648621-36648640 MS.gene068201:CDS 50.0%
TGCTGTGCTCTTGTTGGGTA+AGG + chr1.4:36648741-36648760 MS.gene068201:intron 50.0%
TGGATCAACCCTGGCTTCAT+CGG - chr1.4:36648291-36648310 None:intergenic 50.0%
! CAGCTTGTTTGTAGCAGCCA+AGG - chr1.4:36649215-36649234 None:intergenic 50.0%
! CTTTGTTTGCAATCGCGTGG+TGG + chr1.4:36648688-36648707 MS.gene068201:CDS 50.0%
! TCAATCTCTGAGAGAGGCGA+GGG - chr1.4:36648098-36648117 None:intergenic 50.0%
! TGAATAGTGGTGTAGCGGAG+TGG + chr1.4:36649188-36649207 MS.gene068201:intron 50.0%
!!! AGTCGCCGATTTTTTGCAGG+AGG + chr1.4:36648037-36648056 MS.gene068201:CDS 50.0%
!!! CGAAGTCGCCGATTTTTTGC+AGG + chr1.4:36648034-36648053 MS.gene068201:CDS 50.0%
ACCCTGGCTTCATCGGAAGA+AGG - chr1.4:36648284-36648303 None:intergenic 55.0%
AGCAGACTCCTGCATGGTCA+TGG - chr1.4:36648725-36648744 None:intergenic 55.0%
AGCCTTCTTCCGATGAAGCC+AGG + chr1.4:36648279-36648298 MS.gene068201:CDS 55.0%
ATAGCAGGGAACCCAACAGC+AGG - chr1.4:36649971-36649990 None:intergenic 55.0%
CATCTCGTGCGGCACTTTAG+AGG - chr1.4:36649282-36649301 None:intergenic 55.0%
GAGAAGCCCGAACACTGTCA+TGG - chr1.4:36649407-36649426 None:intergenic 55.0%
GATGCCCAGGAACCATAGCA+GGG - chr1.4:36649985-36650004 None:intergenic 55.0%
GCAGACTCCTGCATGGTCAT+GGG - chr1.4:36648724-36648743 None:intergenic 55.0%
GCCTTCTTCCGATGAAGCCA+GGG + chr1.4:36648280-36648299 MS.gene068201:CDS 55.0%
GGAGTCTGCTGTGCTCTTGT+TGG + chr1.4:36648735-36648754 MS.gene068201:intron 55.0%
GGTTCAGGAGGAACCTCTTG+AGG - chr1.4:36648320-36648339 None:intergenic 55.0%
GGTTCCCTGCTATGGTTCCT+GGG + chr1.4:36649978-36649997 MS.gene068201:CDS 55.0%
GTGGAAGGGGTAGACTGAGT+TGG - chr1.4:36650013-36650032 None:intergenic 55.0%
GTGTCTGCGACGGAAACAGA+AGG - chr1.4:36648074-36648093 None:intergenic 55.0%
TAGCAGGGAACCCAACAGCA+GGG - chr1.4:36649970-36649989 None:intergenic 55.0%
TAGTGCAGCTGCTGCTAGAG+CGG + chr1.4:36649235-36649254 MS.gene068201:intron 55.0%
TCAGTCTACCCCTTCCACTG+TGG + chr1.4:36650015-36650034 MS.gene068201:CDS 55.0%
TGATGCCCAGGAACCATAGC+AGG - chr1.4:36649986-36650005 None:intergenic 55.0%
TGCTGTTGGGTTCCCTGCTA+TGG + chr1.4:36649970-36649989 MS.gene068201:CDS 55.0%
TTGCAATCGCGTGGTGGCTT+TGG + chr1.4:36648694-36648713 MS.gene068201:CDS 55.0%
! CTCAATCTCTGAGAGAGGCG+AGG - chr1.4:36648099-36648118 None:intergenic 55.0%
! TGAGTTGGCACATGATGCCC+AGG - chr1.4:36649998-36650017 None:intergenic 55.0%
!! CGGCTTTGTTTGCAATCGCG+TGG + chr1.4:36648685-36648704 MS.gene068201:CDS 55.0%
CCTCGACCCGATCCCTCAAT+CGG + chr1.4:36648199-36648218 MS.gene068201:CDS 60.0%
GAGCACAGCAGACTCCTGCA+TGG - chr1.4:36648731-36648750 None:intergenic 60.0%
GGGTTCCCTGCTATGGTTCC+TGG + chr1.4:36649977-36649996 MS.gene068201:CDS 60.0%
TGGTGCTCCCATGACCATGC+AGG + chr1.4:36648714-36648733 MS.gene068201:CDS 60.0%
! TCGTGCGGCACTTTAGAGGC+CGG - chr1.4:36649278-36649297 None:intergenic 60.0%
CCGATTGAGGGATCGGGTCG+AGG - chr1.4:36648202-36648221 None:intergenic 65.0%
! TGGTGTAGCGGAGTGGCCCT+TGG + chr1.4:36649195-36649214 MS.gene068201:intron 65.0%
!! AGGCGAGGGAGTGTCTGCGA+CGG - chr1.4:36648084-36648103 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 36647984 36650117 36647984 ID=MS.gene068201
chr1.4 mRNA 36647984 36650117 36647984 ID=MS.gene068201.t1;Parent=MS.gene068201
chr1.4 exon 36647984 36648360 36647984 ID=MS.gene068201.t1.exon1;Parent=MS.gene068201.t1
chr1.4 CDS 36647984 36648360 36647984 ID=cds.MS.gene068201.t1;Parent=MS.gene068201.t1
chr1.4 exon 36648534 36648737 36648534 ID=MS.gene068201.t1.exon2;Parent=MS.gene068201.t1
chr1.4 CDS 36648534 36648737 36648534 ID=cds.MS.gene068201.t1;Parent=MS.gene068201.t1
chr1.4 exon 36649964 36650117 36649964 ID=MS.gene068201.t1.exon3;Parent=MS.gene068201.t1
chr1.4 CDS 36649964 36650117 36649964 ID=cds.MS.gene068201.t1;Parent=MS.gene068201.t1
Gene Sequence

>MS.gene068201

ATGGACAATTTAGAACCCCAAATCGATTTCGAATCTCTCTTCGCAGAATACGAAGTCGCCGATTTTTTGCAGGAGGATGATATCAATCCTTCTGTTTCCGTCGCAGACACTCCCTCGCCTCTCTCAGAGATTGAGAATCTTCTCATGTCTGATGCCGATGGAATTGCGTCGTCATCATCGGATTCCGACTACCATAAGTTTCTCGAAGATATTCTCCTCGACCCGATCCCTCAATCGGATGATCAAGGTTTCGTTCCTTCTGATGAAGCCAGAGTTGATCCAGTAACTCCTCAAGAGCCTTCTTCCGATGAAGCCAGGGTTGATCCAGTAACTCCTCAAGAGGTTCCTCCTGAACCCGTTTCAAAAAAGCTAATTAGGTAAAACACACATAAGTATTAATTAGTCTATAAGAATCATGATTTGTTTTTTTTTTTTTTTTTTTCAACCTGCTGGAGTAATTTGTAATTTGTATTCGAGGTTACCGAATTTGATGGGGTCTTATGGGATGATTATGGTTCTAATGAGAAGTGTTTTGGTATCTGATTTTCAGGCAGATGAGGAACAGGGATGCTGCTGTGAAGTCAAGAGAGAGGAAGAAGATGTACGTGAAAAATCTTGAGACGAAGAGTAGGTATTTTGAAGGGGAATGCAGAAGACTGGAGCATTTGCTTCAGTGCTGCTATGCTGAGAACCATGCTTTGCGGCTTTGTTTGCAATCGCGTGGTGGCTTTGGTGCTCCCATGACCATGCAGGAGTCTGCTGTGCTCTTGTTGGGTAAGGCTTGTGATTGCAAATCAGATTTGCTTTGCTTTGCTTCTTTTTTTATTGTCATTATCATTTTATTTTAGGTATTGTAGTTTAACTTCTAAAAGTATAACCATATGAAGGATTACAGTGACCTTGCACGAATGATTTGGTTTCATTATTGCATTCTTTGGTTGTTTGGCCTAACAATTTTTTTTTCCTTACTTTCTTGAATGGATGGACCTTTTGCTAATGCTATATAGTTTCAATTTCATATATTGGACTTATCATAGTTTCCCAAATTGTAACCTTAATCTCTAATGACTACTGTAGATATATTTATGCCATGTTTTCTTCTTGCTGTTATGTAGATCCGATGTTAAAGACGTCTTTAGGTTTTTTATTGGGTTTTGTAGAACACAAAGTACTGTGTGAATGTATTTTATGTTTAACAGTGTTATGAATAGTGGTGTAGCGGAGTGGCCCTTGGCTGCTACAAACAAGCTGTAGTGCAGCTGCTGCTAGAGCGGCCAATATAGCTCATGTTCCGGCCTCTAAAGTGCCGCACGAGATGAGATCTGAAATTCAAATGAAAAATTTTGACAATAAAAAATCCCATGAAACAGAAAACCTTAGATTTTGGAGTATCTTAGGAATTTTTCAATTATGAAAAACACCATGACAGTGTTCGGGCTTCTCATGCTGTCTCATTCAGGCTATAATATTTCACCTTCTCACTGGTCAGTCACCATAATTAGTGAGTTTATGTATGTACTGGTCATCCCGCTATAGTGTTTTTGAGGTCAGCAACTATGCTATGTTATCTGGGATTGATACATACCACTGCTGTTTAGGTTTTTGCGTCTAAATGGAAAAAAATTGTGTGGGTTGTATGTATAATAATAACAATCACAACCACTTGTTGGTCTTTGTCAGAGTGAAAGTCTACCTCCTGTAGCCTTTTTTTTTTGGTCCAAAGGTGTACCTCCTTTTGCTGTAATCCAGATTTGTGTACTTATGCAAAATACATAAAACATAAACAACATGGAAATTCCAAAACTCTCAGAGAAAAAGTAGATTCCCTTTATGCATGTTAAATGAATAGAAAGTGATTTTGATATGTCAATTCCAATGTTGCCATCATTCGAAGAGTGGGTTTTATGAAGTTGCTCTAATGGAGTTCATGTTAATTAGATCATCATTTATTTACAAATATTGATCATATTCATTTTGCAGAATCCCTGCTGTTGGGTTCCCTGCTATGGTTCCTGGGCATCATGTGCCAACTCAGTCTACCCCTTCCACTGTGGCTAACAACAGTGTTACCTCCAAGAGAAAACATGGAACACAAAGGTCTAAGAAGGGTGGCTCTAAAAGGGCCAAACAGTAA
Protein sequence

>MS.gene068201.t1

MDNLEPQIDFESLFAEYEVADFLQEDDINPSVSVADTPSPLSEIENLLMSDADGIASSSSDSDYHKFLEDILLDPIPQSDDQGFVPSDEARVDPVTPQEPSSDEARVDPVTPQEVPPEPVSKKLIRQMRNRDAAVKSRERKKMYVKNLETKSRYFEGECRRLEHLLQCCYAENHALRLCLQSRGGFGAPMTMQEIPAVGFPAMVPGHHVPTQSTPSTVANNSVTSKRKHGTQRSKKGGSKRAKQ