Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068326.t1 | XP_024632961.1 | 90.9 | 231 | 21 | 0 | 1 | 231 | 81 | 311 | 8.70E-121 | 443 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068326.t1 | Q9SWG3 | 24.7 | 227 | 154 | 5 | 5 | 230 | 81 | 291 | 1.7e-11 | 71.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068326.t1 | A0A072TE13 | 89.6 | 231 | 24 | 0 | 1 | 231 | 66 | 296 | 7.7e-119 | 436.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene068326.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068326.t1 | MTR_0558s0020 | 89.610 | 231 | 24 | 0 | 1 | 231 | 66 | 296 | 1.15e-161 | 449 |
| MS.gene068326.t1 | MTR_6g090505 | 85.652 | 230 | 33 | 0 | 1 | 230 | 1 | 230 | 4.10e-150 | 422 |
| MS.gene068326.t1 | MTR_1g083640 | 90.686 | 204 | 19 | 0 | 1 | 204 | 81 | 284 | 1.12e-143 | 403 |
| MS.gene068326.t1 | MTR_8g446020 | 80.263 | 228 | 45 | 0 | 4 | 231 | 29 | 256 | 1.53e-139 | 391 |
| MS.gene068326.t1 | MTR_2g014100 | 87.209 | 172 | 22 | 0 | 1 | 172 | 74 | 245 | 7.52e-113 | 323 |
| MS.gene068326.t1 | MTR_0337s0040 | 77.720 | 193 | 43 | 0 | 4 | 196 | 30 | 222 | 1.27e-112 | 322 |
| MS.gene068326.t1 | MTR_1g055240 | 88.028 | 142 | 17 | 0 | 27 | 168 | 3 | 144 | 6.26e-93 | 270 |
| MS.gene068326.t1 | MTR_0019s0290 | 82.237 | 152 | 27 | 0 | 18 | 169 | 9 | 160 | 6.28e-92 | 267 |
| MS.gene068326.t1 | MTR_3g008240 | 86.957 | 138 | 18 | 0 | 1 | 138 | 66 | 203 | 6.42e-87 | 256 |
| MS.gene068326.t1 | MTR_4g040430 | 86.232 | 138 | 15 | 1 | 1 | 138 | 65 | 198 | 6.38e-85 | 251 |
| MS.gene068326.t1 | MTR_5g071590 | 83.168 | 101 | 17 | 0 | 1 | 101 | 78 | 178 | 1.09e-61 | 191 |
| MS.gene068326.t1 | MTR_1281s0010 | 82.540 | 63 | 11 | 0 | 1 | 63 | 47 | 109 | 5.31e-32 | 113 |
| MS.gene068326.t1 | MTR_6g051425 | 33.824 | 204 | 123 | 3 | 21 | 224 | 64 | 255 | 3.00e-30 | 115 |
| MS.gene068326.t1 | MTR_1g070355 | 27.053 | 207 | 137 | 2 | 21 | 227 | 119 | 311 | 9.06e-18 | 82.0 |
| MS.gene068326.t1 | MTR_0337s0050 | 75.000 | 36 | 9 | 0 | 195 | 230 | 14 | 49 | 2.06e-14 | 66.6 |
| MS.gene068326.t1 | MTR_0402s0040 | 26.000 | 150 | 96 | 3 | 20 | 169 | 71 | 205 | 5.29e-14 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068326.t1 | AT4G38180 | 30.415 | 217 | 128 | 4 | 20 | 231 | 118 | 316 | 2.85e-26 | 107 |
| MS.gene068326.t1 | AT5G28530 | 23.377 | 231 | 152 | 5 | 5 | 223 | 84 | 301 | 1.46e-12 | 67.0 |
| MS.gene068326.t1 | AT5G28530 | 23.377 | 231 | 152 | 5 | 5 | 223 | 84 | 301 | 1.46e-12 | 67.0 |
| MS.gene068326.t1 | AT5G28530 | 23.377 | 231 | 152 | 5 | 5 | 223 | 84 | 301 | 1.49e-12 | 67.0 |
| MS.gene068326.t1 | AT1G10240 | 24.074 | 216 | 143 | 6 | 21 | 231 | 95 | 294 | 3.64e-12 | 65.9 |
| MS.gene068326.t1 | AT1G10240 | 24.074 | 216 | 143 | 6 | 21 | 231 | 95 | 294 | 3.64e-12 | 65.9 |
| MS.gene068326.t1 | AT4G15090 | 27.586 | 174 | 123 | 3 | 59 | 231 | 121 | 292 | 4.39e-12 | 65.5 |
| MS.gene068326.t1 | AT4G15090 | 27.586 | 174 | 123 | 3 | 59 | 231 | 121 | 292 | 4.39e-12 | 65.5 |
| MS.gene068326.t1 | AT4G15090 | 27.586 | 174 | 123 | 3 | 59 | 231 | 121 | 292 | 4.39e-12 | 65.5 |
| MS.gene068326.t1 | AT4G15090 | 27.586 | 174 | 123 | 3 | 59 | 231 | 121 | 292 | 4.39e-12 | 65.5 |
| MS.gene068326.t1 | AT4G15090 | 27.586 | 174 | 123 | 3 | 59 | 231 | 121 | 292 | 4.39e-12 | 65.5 |
| MS.gene068326.t1 | AT2G27110 | 26.744 | 172 | 109 | 4 | 62 | 231 | 107 | 263 | 6.14e-12 | 65.1 |
| MS.gene068326.t1 | AT2G27110 | 26.744 | 172 | 109 | 4 | 62 | 231 | 107 | 263 | 6.14e-12 | 65.1 |
| MS.gene068326.t1 | AT2G27110 | 33.735 | 83 | 55 | 0 | 149 | 231 | 36 | 118 | 4.88e-11 | 62.4 |
Find 34 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACAAGATTATGCTCTATT+TGG | 0.198611 | 6.3:-69490630 | MS.gene068326:CDS |
| GCCGATATTTATTGTTTGTT+CGG | 0.341758 | 6.3:+69490580 | None:intergenic |
| AACAATAAATATCGGCCATT+AGG | 0.388497 | 6.3:-69490573 | MS.gene068326:CDS |
| ACCGAACAAACAATAAATAT+CGG | 0.389395 | 6.3:-69490581 | MS.gene068326:CDS |
| TTCTCATACTCAACAAGAAC+TGG | 0.395633 | 6.3:+69491110 | None:intergenic |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 6.3:-69490933 | MS.gene068326:CDS |
| AGCTGGAATATATTTGAAGT+TGG | 0.433136 | 6.3:-69491051 | MS.gene068326:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 6.3:-69491068 | MS.gene068326:CDS |
| CGTTATGTTTGTAACAAAGA+AGG | 0.449439 | 6.3:-69491185 | MS.gene068326:CDS |
| GCTTTAATAAATTCTAAAGC+TGG | 0.450264 | 6.3:-69490903 | MS.gene068326:CDS |
| AGTATAAAATACTAACCTAA+TGG | 0.461714 | 6.3:+69490558 | None:intergenic |
| ACAAAATAAGAAATCGGATA+AGG | 0.469178 | 6.3:-69491219 | MS.gene068326:CDS |
| GAAGTGATGGGTCAAGGAGT+TGG | 0.484292 | 6.3:-69490858 | MS.gene068326:CDS |
| TTACAACCAACCCTTTCCTC+TGG | 0.485330 | 6.3:+69491074 | None:intergenic |
| TCTAATGACAACTCGTCAAA+AGG | 0.488137 | 6.3:-69490790 | MS.gene068326:CDS |
| CTCAAGTAGCACAAATGCTC+TGG | 0.489977 | 6.3:+69490963 | None:intergenic |
| CAATTCACAAAATAAGAAAT+CGG | 0.490263 | 6.3:-69491225 | MS.gene068326:CDS |
| CGTCAAAAGGAGATGGTGGC+TGG | 0.492100 | 6.3:-69490777 | MS.gene068326:CDS |
| GAGAACATCTTATGAAGTGA+TGG | 0.499875 | 6.3:-69490871 | MS.gene068326:CDS |
| TGTCTTCTGCAGCATCAACT+TGG | 0.502977 | 6.3:+69490685 | None:intergenic |
| AAATGCTCTGGTTTATGTAA+TGG | 0.504865 | 6.3:+69490975 | None:intergenic |
| TTTAAGTCCGAAAATCGAAA+AGG | 0.508080 | 6.3:+69490819 | None:intergenic |
| TGTCTATAAGATATATAAGT+GGG | 0.514682 | 6.3:-69491015 | MS.gene068326:CDS |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.525236 | 6.3:-69491174 | MS.gene068326:CDS |
| AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | 6.3:-69491173 | MS.gene068326:CDS |
| AATGAAGCTCTCTCGAAACC+AGG | 0.565261 | 6.3:-69490723 | MS.gene068326:CDS |
| GTGATGGGTCAAGGAGTTGG+AGG | 0.568802 | 6.3:-69490855 | MS.gene068326:CDS |
| ATGTCTATAAGATATATAAG+TGG | 0.572287 | 6.3:-69491016 | MS.gene068326:CDS |
| TCTTATGAAGTGATGGGTCA+AGG | 0.589029 | 6.3:-69490864 | MS.gene068326:CDS |
| GACAACTCGTCAAAAGGAGA+TGG | 0.599038 | 6.3:-69490784 | MS.gene068326:CDS |
| AGAACATCTTATGAAGTGAT+GGG | 0.601988 | 6.3:-69490870 | MS.gene068326:CDS |
| AACTCGTCAAAAGGAGATGG+TGG | 0.603142 | 6.3:-69490781 | MS.gene068326:CDS |
| AGAACTGGACTGTCACATGT+TGG | 0.620791 | 6.3:+69491125 | None:intergenic |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.621882 | 6.3:-69490850 | MS.gene068326:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTCTATAAGATATATAAG+TGG | - | chr6.3:69490795-69490814 | MS.gene068326:CDS | 20.0% |
| !! | CAATTCACAAAATAAGAAAT+CGG | - | chr6.3:69490586-69490605 | MS.gene068326:CDS | 20.0% |
| !! | TGTCTATAAGATATATAAGT+GGG | - | chr6.3:69490796-69490815 | MS.gene068326:CDS | 20.0% |
| ! | ACAAAATAAGAAATCGGATA+AGG | - | chr6.3:69490592-69490611 | MS.gene068326:CDS | 25.0% |
| ! | ACCGAACAAACAATAAATAT+CGG | - | chr6.3:69491230-69491249 | MS.gene068326:CDS | 25.0% |
| !!! | CTTATTTTGTGAATTGTTCT+TGG | + | chr6.3:69490582-69490601 | None:intergenic | 25.0% |
| !!! | GCTTTAATAAATTCTAAAGC+TGG | - | chr6.3:69490908-69490927 | MS.gene068326:CDS | 25.0% |
| AACAAAGAAGGTTTCAAGAA+GGG | - | chr6.3:69490638-69490657 | MS.gene068326:CDS | 30.0% | |
| AACAATAAATATCGGCCATT+AGG | - | chr6.3:69491238-69491257 | MS.gene068326:CDS | 30.0% | |
| AGAACATCTTATGAAGTGAT+GGG | - | chr6.3:69490941-69490960 | MS.gene068326:CDS | 30.0% | |
| CAACAAGATTATGCTCTATT+TGG | - | chr6.3:69491181-69491200 | MS.gene068326:CDS | 30.0% | |
| CGTTATGTTTGTAACAAAGA+AGG | - | chr6.3:69490626-69490645 | MS.gene068326:CDS | 30.0% | |
| GCCGATATTTATTGTTTGTT+CGG | + | chr6.3:69491234-69491253 | None:intergenic | 30.0% | |
| TAAAGAAAAACCAGAGGAAA+GGG | - | chr6.3:69490727-69490746 | MS.gene068326:CDS | 30.0% | |
| TAACAAAGAAGGTTTCAAGA+AGG | - | chr6.3:69490637-69490656 | MS.gene068326:CDS | 30.0% | |
| TGGATATCATAGTAAAAACC+TGG | + | chr6.3:69491109-69491128 | None:intergenic | 30.0% | |
| TTTAAGTCCGAAAATCGAAA+AGG | + | chr6.3:69490995-69491014 | None:intergenic | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | + | chr6.3:69490839-69490858 | None:intergenic | 30.0% |
| ! | AGCTGGAATATATTTGAAGT+TGG | - | chr6.3:69490760-69490779 | MS.gene068326:CDS | 30.0% |
| ! | GAATCTTCCTTTTCGATTTT+CGG | - | chr6.3:69490985-69491004 | MS.gene068326:CDS | 30.0% |
| !!! | AAGACATAGCCAGTATTTTT+TGG | - | chr6.3:69491143-69491162 | MS.gene068326:CDS | 30.0% |
| !!! | AGACATAGCCAGTATTTTTT+GGG | - | chr6.3:69491144-69491163 | MS.gene068326:CDS | 30.0% |
| CGAGAAGTAAATGAAGTTCA+AGG | - | chr6.3:69490878-69490897 | MS.gene068326:CDS | 35.0% | |
| CTAAAGAAAAACCAGAGGAA+AGG | - | chr6.3:69490726-69490745 | MS.gene068326:CDS | 35.0% | |
| GAAAGCTAAAGAAAAACCAG+AGG | - | chr6.3:69490721-69490740 | MS.gene068326:CDS | 35.0% | |
| GAGAACATCTTATGAAGTGA+TGG | - | chr6.3:69490940-69490959 | MS.gene068326:CDS | 35.0% | |
| GTCTGCTTAATCCTAAAAAC+AGG | - | chr6.3:69490663-69490682 | MS.gene068326:CDS | 35.0% | |
| TCTAATGACAACTCGTCAAA+AGG | - | chr6.3:69491021-69491040 | MS.gene068326:CDS | 35.0% | |
| TTCTCATACTCAACAAGAAC+TGG | + | chr6.3:69490704-69490723 | None:intergenic | 35.0% | |
| TCCATCTGCCCAAAAAATAC+TGG | + | chr6.3:69491155-69491174 | None:intergenic | 40.0% | |
| !! | TCACATGTTGGCCTGTTTTT+AGG | + | chr6.3:69490677-69490696 | None:intergenic | 40.0% |
| !! | TCTTATGAAGTGATGGGTCA+AGG | - | chr6.3:69490947-69490966 | MS.gene068326:CDS | 40.0% |
| AACTCGTCAAAAGGAGATGG+TGG | - | chr6.3:69491030-69491049 | MS.gene068326:CDS | 45.0% | |
| AATGAAGCTCTCTCGAAACC+AGG | - | chr6.3:69491088-69491107 | MS.gene068326:CDS | 45.0% | |
| AGAACTGGACTGTCACATGT+TGG | + | chr6.3:69490689-69490708 | None:intergenic | 45.0% | |
| CTCAAGTAGCACAAATGCTC+TGG | + | chr6.3:69490851-69490870 | None:intergenic | 45.0% | |
| GAAAAACCAGAGGAAAGGGT+TGG | - | chr6.3:69490731-69490750 | MS.gene068326:CDS | 45.0% | |
| GACAACTCGTCAAAAGGAGA+TGG | - | chr6.3:69491027-69491046 | MS.gene068326:CDS | 45.0% | |
| TGTCTTCTGCAGCATCAACT+TGG | + | chr6.3:69491129-69491148 | None:intergenic | 45.0% | |
| TTACAACCAACCCTTTCCTC+TGG | + | chr6.3:69490740-69490759 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | chr6.3:69490743-69490762 | MS.gene068326:CDS | 45.0% |
| !!! | GCCAGTATTTTTTGGGCAGA+TGG | - | chr6.3:69491151-69491170 | MS.gene068326:CDS | 45.0% |
| !! | GAAGTGATGGGTCAAGGAGT+TGG | - | chr6.3:69490953-69490972 | MS.gene068326:CDS | 50.0% |
| ! | CGTCAAAAGGAGATGGTGGC+TGG | - | chr6.3:69491034-69491053 | MS.gene068326:CDS | 55.0% |
| !! | GTGATGGGTCAAGGAGTTGG+AGG | - | chr6.3:69490956-69490975 | MS.gene068326:CDS | 55.0% |
| GGGTCAAGGAGTTGGAGGCA+CGG | - | chr6.3:69490961-69490980 | MS.gene068326:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 69490569 | 69491264 | 69490569 | ID=MS.gene068326 |
| chr6.3 | mRNA | 69490569 | 69491264 | 69490569 | ID=MS.gene068326.t1;Parent=MS.gene068326 |
| chr6.3 | exon | 69490569 | 69491264 | 69490569 | ID=MS.gene068326.t1.exon1;Parent=MS.gene068326.t1 |
| chr6.3 | CDS | 69490569 | 69491264 | 69490569 | ID=cds.MS.gene068326.t1;Parent=MS.gene068326.t1 |
| Gene Sequence |
| Protein sequence |
