AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068430.t1 MTR_6g008560 69.519 187 56 1 49 234 35 221 2.04e-87 270
MS.gene068430.t1 MTR_5g062230 70.270 185 55 0 50 234 33 217 2.08e-85 265
MS.gene068430.t1 MTR_3g464190 67.204 186 60 1 50 234 36 221 9.86e-81 253
MS.gene068430.t1 MTR_6g039440 65.946 185 63 0 50 234 33 217 2.79e-80 251
MS.gene068430.t1 MTR_5g073200 65.054 186 64 1 50 234 28 213 1.43e-77 244
MS.gene068430.t1 MTR_5g073260 65.241 187 63 2 50 234 30 216 2.50e-76 241
MS.gene068430.t1 MTR_4g048460 58.919 185 76 0 50 234 41 225 5.81e-73 230
MS.gene068430.t1 MTR_4g048460 58.919 185 76 0 50 234 41 225 5.69e-72 230
MS.gene068430.t1 MTR_4g081460 58.857 175 70 1 62 234 52 226 7.78e-64 209
MS.gene068430.t1 MTR_5g094620 56.216 185 62 1 50 234 36 201 3.93e-62 204
MS.gene068430.t1 MTR_2g082010 45.213 188 101 2 49 234 45 232 4.39e-49 170
MS.gene068430.t1 MTR_8g007515 45.251 179 96 1 58 234 41 219 2.93e-46 162
MS.gene068430.t1 MTR_2g081980 43.902 205 107 4 38 234 16 220 5.24e-46 162
MS.gene068430.t1 MTR_2g082060 42.553 188 106 2 49 234 39 226 1.89e-43 155
MS.gene068430.t1 MTR_4g019550 42.487 193 102 3 44 234 42 227 2.38e-42 152
MS.gene068430.t1 MTR_4g045810 38.172 186 109 2 52 234 62 244 4.95e-33 126
MS.gene068430.t1 MTR_2g082050 37.430 179 88 3 57 234 1 156 1.74e-30 118
MS.gene068430.t1 MTR_2g064425 41.954 174 95 3 62 234 83 251 3.23e-29 114
MS.gene068430.t1 MTR_2g064425 41.954 174 95 3 62 234 83 251 9.22e-29 114
MS.gene068430.t1 MTR_2g089130 32.821 195 117 3 44 235 48 231 3.34e-28 112
MS.gene068430.t1 MTR_6g005210 96.226 53 2 0 1 53 187 239 2.23e-27 110
MS.gene068430.t1 MTR_2g089120 35.260 173 104 2 63 235 67 231 4.31e-27 109
MS.gene068430.t1 MTR_2g065450 36.257 171 102 3 66 234 90 255 1.84e-24 102
MS.gene068430.t1 MTR_8g057020 34.503 171 107 2 65 234 87 253 4.11e-24 101
MS.gene068430.t1 MTR_4g092020 34.091 176 109 3 58 233 64 232 6.79e-24 100
MS.gene068430.t1 MTR_4g097810 63.158 76 23 2 1 76 7 77 1.99e-23 91.3
MS.gene068430.t1 MTR_6g008560 70.769 65 19 0 170 234 1 65 1.91e-22 95.5
MS.gene068430.t1 MTR_1g028230 87.500 48 6 0 6 53 91 138 4.99e-22 92.0
MS.gene068430.t1 MTR_4g092010 34.756 164 104 3 69 229 61 224 1.68e-21 93.6
MS.gene068430.t1 MTR_2g010960 32.258 186 112 4 56 232 2 182 9.20e-21 91.7
MS.gene068430.t1 MTR_2g064425 36.782 174 89 4 62 234 83 236 1.30e-19 87.8
MS.gene068430.t1 MTR_2g064425 36.782 174 89 4 62 234 83 236 1.67e-19 87.8
MS.gene068430.t1 MTR_7g094970 31.414 191 109 5 82 256 206 390 2.45e-17 81.6
MS.gene068430.t1 MTR_6g064980 32.571 175 115 3 68 240 13 186 3.17e-16 78.2
MS.gene068430.t1 MTR_7g011663 29.897 194 114 5 82 259 344 531 4.21e-16 78.2
MS.gene068430.t1 MTR_7g011770 29.843 191 112 6 82 256 240 424 5.85e-15 74.7
MS.gene068430.t1 MTR_3g063170 30.460 174 118 3 68 239 13 185 6.06e-15 74.3
MS.gene068430.t1 MTR_7g010710 28.090 178 121 4 62 236 47 220 1.25e-14 73.6
MS.gene068430.t1 MTR_3g465090 32.934 167 109 3 68 232 13 178 1.30e-14 73.6
MS.gene068430.t1 MTR_5g030050 32.222 180 100 5 87 256 3 170 3.02e-14 71.6
MS.gene068430.t1 MTR_3g063160 30.286 175 119 3 68 240 13 186 4.49e-14 70.9
MS.gene068430.t1 MTR_5g094530 63.793 58 17 2 180 234 1 57 5.44e-14 68.9
MS.gene068430.t1 MTR_3g052120 28.191 188 128 4 66 251 11 193 1.10e-13 70.9
MS.gene068430.t1 MTR_3g058160 31.847 157 97 4 84 231 256 411 2.36e-13 70.1
MS.gene068430.t1 MTR_3g058160 31.847 157 97 4 84 231 65 220 2.45e-13 69.7
MS.gene068430.t1 MTR_3g058160 31.847 157 97 4 84 231 276 431 2.47e-13 69.7
MS.gene068430.t1 MTR_3g058160 31.847 157 97 4 84 231 269 424 2.56e-13 69.7
MS.gene068430.t1 MTR_3g058160 31.847 157 97 4 84 231 276 431 2.91e-13 69.7
MS.gene068430.t1 MTR_3g063140 29.714 175 120 3 68 240 13 186 9.18e-13 68.2
MS.gene068430.t1 MTR_3g063120 29.630 162 111 2 82 240 1 162 3.97e-12 66.2
MS.gene068430.t1 MTR_6g090470 56.000 50 22 0 3 52 172 221 9.50e-12 64.7
MS.gene068430.t1 MTR_4g125800 52.000 50 24 0 1 50 118 167 4.22e-11 62.4
MS.gene068430.t1 MTR_4g125800 52.000 50 24 0 1 50 171 220 6.30e-11 62.0
MS.gene068430.t1 MTR_4g125800 52.000 50 24 0 1 50 194 243 6.98e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068430.t1 AT5G23960 39.766 171 100 2 66 234 41 210 3.16e-38 140
MS.gene068430.t1 AT5G23960 39.766 171 100 2 66 234 41 210 3.91e-38 140
MS.gene068430.t1 AT1G31950 36.316 190 106 4 57 234 84 270 4.27e-31 120
MS.gene068430.t1 AT1G31950 36.316 190 106 4 57 234 84 270 7.56e-31 120
MS.gene068430.t1 AT3G14540 37.288 177 105 2 62 234 90 264 1.37e-30 119
MS.gene068430.t1 AT3G14540 37.288 177 105 2 62 234 90 264 2.71e-30 119
MS.gene068430.t1 AT3G29410 36.869 198 115 4 40 234 76 266 4.87e-30 118
MS.gene068430.t1 AT5G44630 39.344 183 96 6 62 234 43 220 5.19e-30 118
MS.gene068430.t1 AT1G31950 36.559 186 103 4 61 234 87 269 6.37e-30 118
MS.gene068430.t1 AT1G70080 37.989 179 104 4 62 234 96 273 6.52e-30 118
MS.gene068430.t1 AT4G13300 36.318 201 116 6 39 234 23 216 6.63e-29 115
MS.gene068430.t1 AT4G15870 38.378 185 106 5 56 234 90 272 1.13e-28 114
MS.gene068430.t1 AT3G14520 35.955 178 106 3 62 234 93 267 1.50e-28 114
MS.gene068430.t1 AT3G14490 36.416 173 104 2 66 234 95 265 2.30e-28 113
MS.gene068430.t1 AT1G33750 35.859 198 117 4 40 234 78 268 3.90e-28 112
MS.gene068430.t1 AT4G20200 34.483 203 117 6 39 234 73 266 6.59e-28 112
MS.gene068430.t1 AT3G29410 38.037 163 98 2 73 234 1 161 5.20e-27 109
MS.gene068430.t1 AT3G29190 33.333 177 112 3 62 234 89 263 6.52e-27 109
MS.gene068430.t1 AT1G48800 36.111 180 105 4 62 235 93 268 9.73e-27 108
MS.gene068430.t1 AT4G20210 35.754 179 105 4 62 234 88 262 1.11e-26 108
MS.gene068430.t1 AT4G20230 33.520 179 109 4 62 234 104 278 2.23e-26 107
MS.gene068430.t1 AT4G20230 33.520 179 109 4 62 234 92 266 3.06e-26 107
MS.gene068430.t1 AT3G29110 37.640 178 105 5 62 235 85 260 9.74e-26 106
MS.gene068430.t1 AT3G32030 34.673 199 120 6 40 234 75 267 1.25e-25 105
MS.gene068430.t1 AT4G13280 35.323 201 118 6 39 234 23 216 1.35e-24 102
MS.gene068430.t1 AT1G66020 33.898 177 111 3 62 234 86 260 1.54e-24 101
MS.gene068430.t1 AT4G13280 35.323 201 118 6 39 234 23 216 1.87e-24 102
MS.gene068430.t1 AT1G66020 33.898 177 111 3 62 234 86 260 2.44e-24 102
MS.gene068430.t1 AT5G40440 84.906 53 8 0 1 53 104 156 3.99e-24 100
MS.gene068430.t1 AT5G40440 84.906 53 8 0 1 53 186 238 4.01e-24 101
MS.gene068430.t1 AT5G40440 84.906 53 8 0 1 53 186 238 4.01e-24 101
MS.gene068430.t1 AT5G40440 84.906 53 8 0 1 53 168 220 4.40e-24 100
MS.gene068430.t1 AT5G40440 84.906 53 8 0 1 53 168 220 4.40e-24 100
MS.gene068430.t1 AT5G40440 84.906 53 8 0 1 53 168 220 4.40e-24 100
MS.gene068430.t1 AT4G16730 34.043 188 111 4 56 234 17 200 2.42e-23 99.0
MS.gene068430.t1 AT1G61680 33.533 167 104 2 68 233 77 237 5.47e-22 95.1
MS.gene068430.t1 AT2G23230 32.222 180 111 5 62 234 86 261 4.36e-21 92.8
MS.gene068430.t1 AT2G23230 32.222 180 111 5 62 234 86 261 4.72e-21 92.4
MS.gene068430.t1 AT4G16730 35.032 157 90 3 87 234 1 154 8.46e-20 88.6
MS.gene068430.t1 AT4G16740 33.889 180 102 5 66 235 60 232 5.63e-19 85.5
MS.gene068430.t1 AT4G16740 33.889 180 102 5 66 235 60 232 5.63e-19 85.5
MS.gene068430.t1 AT4G16740 32.447 188 110 5 58 235 52 232 5.98e-19 85.9
MS.gene068430.t1 AT1G61680 32.335 167 99 3 68 233 77 230 7.77e-19 85.9
MS.gene068430.t1 AT4G16740 33.889 180 102 5 66 235 60 232 1.22e-18 85.5
MS.gene068430.t1 AT2G24210 32.584 178 107 4 64 234 78 249 6.08e-18 83.6
MS.gene068430.t1 AT1G48820 32.386 176 91 5 62 235 93 242 1.75e-17 82.0
MS.gene068430.t1 AT4G02780 30.729 192 109 6 82 256 329 513 5.98e-17 80.5
MS.gene068430.t1 AT3G25820 32.704 159 100 4 35 191 53 206 9.96e-17 79.3
MS.gene068430.t1 AT3G25820 32.704 159 100 4 35 191 53 206 9.96e-17 79.3
MS.gene068430.t1 AT3G25820 32.704 159 100 4 35 191 53 206 1.46e-16 79.0
MS.gene068430.t1 AT3G25830 32.704 159 100 4 35 191 53 206 1.46e-16 79.3
MS.gene068430.t1 AT3G25820 32.704 159 100 4 35 191 53 206 1.46e-16 79.3
MS.gene068430.t1 AT3G25810 34.711 121 67 4 77 191 93 207 3.28e-13 69.3
MS.gene068430.t1 AT4G29810 50.943 53 26 0 1 53 180 232 2.91e-12 66.2
MS.gene068430.t1 AT4G29810 50.943 53 26 0 1 53 171 223 3.32e-12 65.9
MS.gene068430.t1 AT4G29810 50.943 53 26 0 1 53 180 232 3.59e-12 65.9
MS.gene068430.t1 AT5G56580 56.000 50 22 0 3 52 174 223 1.29e-11 64.3
MS.gene068430.t1 AT4G26070 56.818 44 19 0 1 44 156 199 6.88e-11 62.0
MS.gene068430.t1 AT4G26070 56.818 44 19 0 1 44 169 212 6.96e-11 62.0
MS.gene068430.t1 AT4G26070 56.818 44 19 0 1 44 168 211 6.98e-11 62.0
MS.gene068430.t1 AT4G26070 56.818 44 19 0 1 44 169 212 7.83e-11 62.0
MS.gene068430.t1 AT4G26070 56.818 44 19 0 1 44 169 212 7.83e-11 62.0

Find 43 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTCTTGCTGTGCTATTT+AGG 0.103456 6.3:-64198152 MS.gene068430:CDS
TTCTCTTGCTGTGCTATTTA+GGG 0.189824 6.3:-64198151 MS.gene068430:CDS
GAGCCGAAAATTACTGATTT+TGG 0.210770 6.3:-64199371 MS.gene068430:CDS
AGCCTACATCAAAAGGAATT+TGG 0.257080 6.3:-64197160 MS.gene068430:CDS
TACAAGAGTTATGTTCAAAA+TGG 0.269625 6.3:-64198201 MS.gene068430:CDS
AGACGAAATTAATCAAGTTT+TGG 0.301364 6.3:-64198232 MS.gene068430:CDS
TTCCTTCCTCGTCCTTGAAT+TGG 0.345275 6.3:+64197488 None:intergenic
TGTGCCGCAGTATTCATATC+AGG 0.354426 6.3:+64198372 None:intergenic
GATTGATTCAATATGTCGTT+TGG 0.383797 6.3:-64198274 MS.gene068430:CDS
ATTTACTTGTAAACCTCAAA+GGG 0.420841 6.3:-64199394 MS.gene068430:CDS
TTCTGGTCACCTTGAGTCCA+TGG 0.446304 6.3:-64197356 MS.gene068430:CDS
ATTGATTCAATATGTCGTTT+GGG 0.450241 6.3:-64198273 MS.gene068430:CDS
GCTCCAAAGCCTACATCAAA+AGG 0.450495 6.3:-64197167 MS.gene068430:intron
GTTTGAAGCATCACATATGA+TGG 0.470128 6.3:-64197419 MS.gene068430:CDS
TTGCAAGAGAAGGACTCAAT+CGG 0.484754 6.3:+64197329 None:intergenic
AGGTTTACAAGTAAATTAGC+AGG 0.487423 6.3:+64199401 None:intergenic
AATTTACTTGTAAACCTCAA+AGG 0.501450 6.3:-64199395 MS.gene068430:CDS
CTGTGCTATTTAGGGTGTTG+AGG 0.502767 6.3:-64198143 MS.gene068430:CDS
CATGGACTTTACGTTTCACC+TGG 0.512238 6.3:-64198117 MS.gene068430:intron
AGGACTCAATCGGGTGGCCA+TGG 0.512884 6.3:+64197339 None:intergenic
AGATGTGTTCAACCAATTCA+AGG 0.513117 6.3:-64197500 MS.gene068430:CDS
TAACTTCTTGCCACTAGCCT+AGG 0.521919 6.3:+64197262 None:intergenic
ATATAGGTGGTGGAAGGAGT+TGG 0.524102 6.3:-64197028 MS.gene068430:intron
AACATTGGTCAGAGATGTTG+AGG 0.533818 6.3:-64197455 MS.gene068430:CDS
GCATCACATATGATGGTTCA+TGG 0.537961 6.3:-64197412 MS.gene068430:CDS
TGCAAGAGAAGGACTCAATC+GGG 0.546913 6.3:+64197330 None:intergenic
TTTAGGGTGTTGAGGCAACA+TGG 0.552007 6.3:-64198135 MS.gene068430:CDS
TTTACTTGTGTTGCAAGAGA+AGG 0.558925 6.3:+64197319 None:intergenic
CATAGAGAATTCAGTTGCAA+TGG 0.563989 6.3:-64199336 MS.gene068430:intron
ATCGGGTGGCCATGGACTCA+AGG 0.566605 6.3:+64197347 None:intergenic
ACTACTCTATCTTGTGCATA+CGG 0.570577 6.3:+64196988 None:intergenic
ATAGCACAGCAAGAGAATGA+AGG 0.572946 6.3:+64198157 None:intergenic
ACGTAGGAGCTTCTGAAACA+TGG 0.573800 6.3:+64199579 None:intergenic
AGGAAACTTCAGTGAAACAT+TGG 0.576091 6.3:-64197470 MS.gene068430:CDS
CTTTATATATAGGTGGTGGA+AGG 0.588928 6.3:-64197034 MS.gene068430:intron
AACCAATTCAAGGACGAGGA+AGG 0.615055 6.3:-64197490 MS.gene068430:CDS
GTTCAACCAATTCAAGGACG+AGG 0.652307 6.3:-64197494 MS.gene068430:CDS
AAGGGTCTAAGTTATCTGCA+CGG 0.655190 6.3:-64199455 MS.gene068430:CDS
ACATTGGTCAGAGATGTTGA+GGG 0.666287 6.3:-64197454 MS.gene068430:CDS
TTTACTTGTAAACCTCAAAG+GGG 0.694553 6.3:-64199393 MS.gene068430:CDS
AAGAGAAGGACTCAATCGGG+TGG 0.695745 6.3:+64197333 None:intergenic
GGAACCTGATATGAATACTG+CGG 0.696636 6.3:-64198376 MS.gene068430:CDS
AAAGCTTAAGACAATCTCTG+CGG 0.738710 6.3:-64197294 MS.gene068430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAATTAAAAGAAATG+AGG + chr6.3:64198816-64198835 None:intergenic 10.0%
!!! CATTTTTTATGTTTTTAATT+TGG - chr6.3:64198505-64198524 MS.gene068430:intron 10.0%
!!! TATTTTTTGTCTTTATATAT+AGG - chr6.3:64199522-64199541 MS.gene068430:intron 10.0%
!! ACAAATTGATAAACTAAAAA+AGG - chr6.3:64198214-64198233 MS.gene068430:CDS 15.0%
!! ATAAAGACAAAAAATAGTAT+AGG + chr6.3:64199518-64199537 None:intergenic 15.0%
!! TAATATGAATGTCTAAAAAA+TGG - chr6.3:64198637-64198656 MS.gene068430:intron 15.0%
!!! ACTGTATTAAGTATTAATTT+TGG - chr6.3:64197586-64197605 MS.gene068430:intron 15.0%
!!! ATACTTTTATATTTTGATGT+AGG + chr6.3:64199460-64199479 None:intergenic 15.0%
!!! ATTAGATAGATATTTCTTTT+AGG + chr6.3:64197649-64197668 None:intergenic 15.0%
!!! ATTGTTGTTTAATATTATTG+TGG - chr6.3:64199025-64199044 MS.gene068430:intron 15.0%
!!! CTGTATTAAGTATTAATTTT+GGG - chr6.3:64197587-64197606 MS.gene068430:intron 15.0%
!!! GTTTTTTTAATCTTTTTGAA+GGG - chr6.3:64197093-64197112 MS.gene068430:intron 15.0%
!!! TTAGATAGATATTTCTTTTA+GGG + chr6.3:64197648-64197667 None:intergenic 15.0%
!! AATTGATAAACTAAAAAAGG+AGG - chr6.3:64198217-64198236 MS.gene068430:CDS 20.0%
!! ATGCAAGTAAAATTTCATTA+TGG + chr6.3:64199357-64199376 None:intergenic 20.0%
!! TAATATTAATGTATACATGC+AGG - chr6.3:64198875-64198894 MS.gene068430:intron 20.0%
!! TGCAAGTAAAATTTCATTAT+GGG + chr6.3:64199356-64199375 None:intergenic 20.0%
!! TTGAGTAAAACAAATTAGAT+TGG - chr6.3:64198536-64198555 MS.gene068430:intron 20.0%
!!! ATACCAAAATCAGTAATTTT+CGG + chr6.3:64197201-64197220 None:intergenic 20.0%
!!! CTTTTTACTAAAAGATTTAC+AGG + chr6.3:64198677-64198696 None:intergenic 20.0%
!!! GGTTTTTTTAATCTTTTTGA+AGG - chr6.3:64197092-64197111 MS.gene068430:intron 20.0%
!!! TAATGAAATTTTACTTGCAT+TGG - chr6.3:64199357-64199376 MS.gene068430:CDS 20.0%
!!! TTTTTGTCTTTATATATAGG+TGG - chr6.3:64199525-64199544 MS.gene068430:intron 20.0%
! AATTTACTTGTAAACCTCAA+AGG - chr6.3:64197171-64197190 MS.gene068430:intron 25.0%
! ATATGAACATTACACAGATA+AGG + chr6.3:64198793-64198812 None:intergenic 25.0%
! ATTTACTTGTAAACCTCAAA+GGG - chr6.3:64197172-64197191 MS.gene068430:intron 25.0%
! TACAAGAGTTATGTTCAAAA+TGG - chr6.3:64198365-64198384 MS.gene068430:CDS 25.0%
!! AGACGAAATTAATCAAGTTT+TGG - chr6.3:64198334-64198353 MS.gene068430:CDS 25.0%
!! ATGACACTTAATTGTTGTTA+TGG + chr6.3:64198125-64198144 None:intergenic 25.0%
!! ATTGATTCAATATGTCGTTT+GGG - chr6.3:64198293-64198312 MS.gene068430:CDS 25.0%
!! GTAATATTTGACTTTGTCAT+GGG - chr6.3:64198608-64198627 MS.gene068430:intron 25.0%
!! TAAGTTGAGATATATATACC+AGG + chr6.3:64198470-64198489 None:intergenic 25.0%
!! TTAATTTTGGGCCATAAAAA+TGG - chr6.3:64197599-64197618 MS.gene068430:intron 25.0%
!!! ATAGATAGTAGCCATTTTTA+TGG + chr6.3:64197613-64197632 None:intergenic 25.0%
!!! TGTATGTTTTGAATACATAC+AGG - chr6.3:64198169-64198188 MS.gene068430:CDS 25.0%
AGGTTTACAAGTAAATTAGC+AGG + chr6.3:64197168-64197187 None:intergenic 30.0%
ATTGAGATATGCTACTAACT+TGG - chr6.3:64197067-64197086 MS.gene068430:intron 30.0%
GAAGTGCAATTTCTATCTAT+AGG + chr6.3:64197460-64197479 None:intergenic 30.0%
GGTTCATGGTGAAAAAATAT+TGG - chr6.3:64199168-64199187 MS.gene068430:intron 30.0%
TGGTGAAAAAATATTGGAAG+AGG - chr6.3:64199174-64199193 MS.gene068430:intron 30.0%
TTGTCTTTATATATAGGTGG+TGG - chr6.3:64199528-64199547 MS.gene068430:intron 30.0%
TTTACTTGTAAACCTCAAAG+GGG - chr6.3:64197173-64197192 MS.gene068430:intron 30.0%
! CGTAATATTTGACTTTGTCA+TGG - chr6.3:64198607-64198626 MS.gene068430:intron 30.0%
! GATTGATTCAATATGTCGTT+TGG - chr6.3:64198292-64198311 MS.gene068430:CDS 30.0%
! TTCCAAATTCCTTTTGATGT+AGG + chr6.3:64199411-64199430 None:intergenic 30.0%
! TTTACTTGCATTGGCAAAAT+TGG - chr6.3:64199366-64199385 MS.gene068430:CDS 30.0%
!! ACTGATTTTGGTATTAGTGT+TGG - chr6.3:64197207-64197226 MS.gene068430:intron 30.0%
!!! AATCAAGTTGACTTTTTCCA+TGG + chr6.3:64198276-64198295 None:intergenic 30.0%
!!! ATCAAGTTGACTTTTTCCAT+GGG + chr6.3:64198275-64198294 None:intergenic 30.0%
AAAGATTTACAGGCTAACAG+TGG + chr6.3:64198667-64198686 None:intergenic 35.0%
AAAGCTTAAGACAATCTCTG+CGG - chr6.3:64199272-64199291 MS.gene068430:intron 35.0%
AACAAAGTGTGGAAGCAAAT+CGG + chr6.3:64197296-64197315 None:intergenic 35.0%
AATATATGCTCCTTACACGT+AGG + chr6.3:64197006-64197025 None:intergenic 35.0%
AGATATGCTACTAACTTGGT+TGG - chr6.3:64197071-64197090 MS.gene068430:intron 35.0%
AGATGTGTTCAACCAATTCA+AGG - chr6.3:64199066-64199085 MS.gene068430:intron 35.0%
AGCCTACATCAAAAGGAATT+TGG - chr6.3:64199406-64199425 MS.gene068430:CDS 35.0%
AGGAAACTTCAGTGAAACAT+TGG - chr6.3:64199096-64199115 MS.gene068430:intron 35.0%
CATAGAGAATTCAGTTGCAA+TGG - chr6.3:64197230-64197249 MS.gene068430:intron 35.0%
TGGTAAAATGCTAAAAGCCT+TGG + chr6.3:64198022-64198041 None:intergenic 35.0%
TGTTGTTATGGATCAACCTA+TGG + chr6.3:64198113-64198132 None:intergenic 35.0%
TTTACTTGTGTTGCAAGAGA+AGG + chr6.3:64199250-64199269 None:intergenic 35.0%
! AAATTCGTACACATTTTGCC+AGG - chr6.3:64197759-64197778 MS.gene068430:intron 35.0%
! AAGAAATGTTGCATTTTGCC+AGG + chr6.3:64198056-64198075 None:intergenic 35.0%
! AAGGCTTTTAGCATTTTACC+AGG - chr6.3:64198021-64198040 MS.gene068430:intron 35.0%
! AATTCGTACACATTTTGCCA+GGG - chr6.3:64197760-64197779 MS.gene068430:intron 35.0%
! AGAAATGTTGCATTTTGCCA+GGG + chr6.3:64198055-64198074 None:intergenic 35.0%
! AGGCTTTTAGCATTTTACCA+GGG - chr6.3:64198022-64198041 MS.gene068430:intron 35.0%
! ATTCCTTTTGATGTAGGCTT+TGG + chr6.3:64199405-64199424 None:intergenic 35.0%
! GTTTGAAGCATCACATATGA+TGG - chr6.3:64199147-64199166 MS.gene068430:intron 35.0%
! TTTTGCTAAATGCAGTCCAT+AGG - chr6.3:64198094-64198113 MS.gene068430:intron 35.0%
!! AGTCATTTTCAAAACATGCC+AGG + chr6.3:64197805-64197824 None:intergenic 35.0%
!! ATTCTCTTGCTGTGCTATTT+AGG - chr6.3:64198414-64198433 MS.gene068430:intron 35.0%
!! CTTTATATATAGGTGGTGGA+AGG - chr6.3:64199532-64199551 MS.gene068430:intron 35.0%
!! GTGTTTCTGAAGTACGAAAA+AGG + chr6.3:64198918-64198937 None:intergenic 35.0%
!! TTCTCTTGCTGTGCTATTTA+GGG - chr6.3:64198415-64198434 MS.gene068430:intron 35.0%
!!! GAGCCGAAAATTACTGATTT+TGG - chr6.3:64197195-64197214 MS.gene068430:intron 35.0%
AACATTGGTCAGAGATGTTG+AGG - chr6.3:64199111-64199130 MS.gene068430:intron 40.0%
AAGGGTCTAAGTTATCTGCA+CGG - chr6.3:64197111-64197130 MS.gene068430:intron 40.0%
ACATTGGTCAGAGATGTTGA+GGG - chr6.3:64199112-64199131 MS.gene068430:intron 40.0%
ATAGCACAGCAAGAGAATGA+AGG + chr6.3:64198412-64198431 None:intergenic 40.0%
CTCAAAAACAGAGAAACCCA+TGG - chr6.3:64198256-64198275 MS.gene068430:CDS 40.0%
GCATCACATATGATGGTTCA+TGG - chr6.3:64199154-64199173 MS.gene068430:intron 40.0%
GCTCAACAAGATCCTCAATA+CGG + chr6.3:64197498-64197517 None:intergenic 40.0%
GGAACCTGATATGAATACTG+CGG - chr6.3:64198190-64198209 MS.gene068430:CDS 40.0%
GGATGAATCTTGCTCATAGA+TGG + chr6.3:64199335-64199354 None:intergenic 40.0%
TCAGTCAAGCAAACAAAGTG+TGG + chr6.3:64197307-64197326 None:intergenic 40.0%
TTGCAAGAGAAGGACTCAAT+CGG + chr6.3:64199240-64199259 None:intergenic 40.0%
! AAAAATATTGGAAGAGGCGC+TGG - chr6.3:64199180-64199199 MS.gene068430:intron 40.0%
! ATTCGTACACATTTTGCCAG+GGG - chr6.3:64197761-64197780 MS.gene068430:intron 40.0%
! GAAATGTTGCATTTTGCCAG+GGG + chr6.3:64198054-64198073 None:intergenic 40.0%
!! AGGAGTGTCTTTCGTTTGAT+CGG - chr6.3:64198750-64198769 MS.gene068430:intron 40.0%
AACCAATTCAAGGACGAGGA+AGG - chr6.3:64199076-64199095 MS.gene068430:intron 45.0%
AAGCTGACACAATTTGCCAG+GGG - chr6.3:64197873-64197892 MS.gene068430:intron 45.0%
AGATGAGCAAGTCCGTATTG+AGG - chr6.3:64197483-64197502 MS.gene068430:CDS 45.0%
ATGCTAAAAGCCTTGGCAAC+TGG + chr6.3:64198015-64198034 None:intergenic 45.0%
CAAGCTGACACAATTTGCCA+GGG - chr6.3:64197872-64197891 MS.gene068430:intron 45.0%
CATGGACTTTACGTTTCACC+TGG - chr6.3:64198449-64198468 MS.gene068430:intron 45.0%
GAAAAACTTGCCTAGGCTAG+TGG - chr6.3:64199294-64199313 MS.gene068430:intron 45.0%
GCTCCAAAGCCTACATCAAA+AGG - chr6.3:64199399-64199418 MS.gene068430:CDS 45.0%
GTTCAACCAATTCAAGGACG+AGG - chr6.3:64199072-64199091 MS.gene068430:intron 45.0%
TGCAAGAGAAGGACTCAATC+GGG + chr6.3:64199239-64199258 None:intergenic 45.0%
TGTGCCGCAGTATTCATATC+AGG + chr6.3:64198197-64198216 None:intergenic 45.0%
TTCAGAAGCTCCTACGTGTA+AGG - chr6.3:64196993-64197012 MS.gene068430:CDS 45.0%
TTCCTTCCTCGTCCTTGAAT+TGG + chr6.3:64199081-64199100 None:intergenic 45.0%
TTTGCGATGAGCAGATTTGC+TGG - chr6.3:64197955-64197974 MS.gene068430:intron 45.0%
! TAACTTCTTGCCACTAGCCT+AGG + chr6.3:64199307-64199326 None:intergenic 45.0%
! TAATTTTCGGCTCCCCTTTG+AGG + chr6.3:64197188-64197207 None:intergenic 45.0%
!! ATATAGGTGGTGGAAGGAGT+TGG - chr6.3:64199538-64199557 MS.gene068430:intron 45.0%
!! CTGTGCTATTTAGGGTGTTG+AGG - chr6.3:64198423-64198442 MS.gene068430:intron 45.0%
!! TTTAGGGTGTTGAGGCAACA+TGG - chr6.3:64198431-64198450 MS.gene068430:intron 45.0%
!!! ATTATTTTTTTACATAAAAT+AGG - chr6.3:64198730-64198749 MS.gene068430:intron 5.0%
AAGAGAAGGACTCAATCGGG+TGG + chr6.3:64199236-64199255 None:intergenic 50.0%
ATCGCAAATTGTCCTGCCAG+GGG + chr6.3:64197943-64197962 None:intergenic 50.0%
CATCGCAAATTGTCCTGCCA+GGG + chr6.3:64197944-64197963 None:intergenic 50.0%
CTCTGCGGAAAAACTTGCCT+AGG - chr6.3:64199287-64199306 MS.gene068430:intron 50.0%
GACACAATTTGCCAGGGGTT+AGG - chr6.3:64197878-64197897 MS.gene068430:intron 50.0%
GCAAGCTGACACAATTTGCC+AGG - chr6.3:64197871-64197890 MS.gene068430:intron 50.0%
TCATCGCAAATTGTCCTGCC+AGG + chr6.3:64197945-64197964 None:intergenic 50.0%
TTCTGGTCACCTTGAGTCCA+TGG - chr6.3:64199210-64199229 MS.gene068430:intron 50.0%
TTTGCTGGTGATTCTGCTCC+TGG - chr6.3:64197970-64197989 MS.gene068430:intron 50.0%
! CAGCTTGCATGCAGCTTTTC+TGG + chr6.3:64197859-64197878 None:intergenic 50.0%
! TTTTCCAGGAGCTAAACCCC+TGG - chr6.3:64197924-64197943 MS.gene068430:intron 50.0%
!! CAGCTTTTCTGGCATTGTGC+AGG + chr6.3:64197848-64197867 None:intergenic 50.0%
AATTTGCCAGGGGTTAGGCC+AGG - chr6.3:64197883-64197902 MS.gene068430:intron 55.0%
ATTTGCCAGGGGTTAGGCCA+GGG - chr6.3:64197884-64197903 MS.gene068430:intron 55.0%
TGGCAAACGTCCAGTTGCCA+AGG - chr6.3:64198002-64198021 MS.gene068430:intron 55.0%
TGGCAACTGGACGTTTGCCA+GGG + chr6.3:64198002-64198021 None:intergenic 55.0%
TTAACCCCTGGCAATGCTCC+TGG - chr6.3:64197784-64197803 MS.gene068430:intron 55.0%
TTGGCAACTGGACGTTTGCC+AGG + chr6.3:64198003-64198022 None:intergenic 55.0%
! TTTTGCCAGGGGTTAACCCC+TGG - chr6.3:64197772-64197791 MS.gene068430:intron 55.0%
!! AACATGCCAGGAGCATTGCC+AGG + chr6.3:64197793-64197812 None:intergenic 55.0%
!! ACATGCCAGGAGCATTGCCA+GGG + chr6.3:64197792-64197811 None:intergenic 55.0%
!!! GAGGCGCTGGCTTTTACTTC+TGG - chr6.3:64199193-64199212 MS.gene068430:intron 55.0%
AGGACTCAATCGGGTGGCCA+TGG + chr6.3:64199230-64199249 None:intergenic 60.0%
ATCGGGTGGCCATGGACTCA+AGG + chr6.3:64199222-64199241 None:intergenic 60.0%
CATTGCCAGGGGTTAACCCC+TGG + chr6.3:64197780-64197799 None:intergenic 60.0%
GCTCCTGGAAAATGCGCTCC+TGG + chr6.3:64197916-64197935 None:intergenic 60.0%
GGAAAATGCGCTCCTGGCAC+AGG + chr6.3:64197910-64197929 None:intergenic 60.0%
GGCAACTGGACGTTTGCCAG+GGG + chr6.3:64198001-64198020 None:intergenic 60.0%
TCTGCTCCTGGCAAAACCCC+TGG - chr6.3:64197982-64198001 MS.gene068430:intron 60.0%
TTTACCAGGGCTTCTGCCCC+TGG - chr6.3:64198035-64198054 MS.gene068430:intron 60.0%
! CGTTTGCCAGGGGTTTTGCC+AGG + chr6.3:64197991-64198010 None:intergenic 60.0%
!! CATGCCAGGAGCATTGCCAG+GGG + chr6.3:64197791-64197810 None:intergenic 60.0%
!! GTGCCAGGAGCGCATTTTCC+AGG - chr6.3:64197910-64197929 MS.gene068430:intron 60.0%
CCAGGAGCTAAACCCCTGGC+AGG - chr6.3:64197928-64197947 MS.gene068430:intron 65.0%
TTTGCCAGGGGCAGAAGCCC+TGG + chr6.3:64198042-64198061 None:intergenic 65.0%
! CCTGCCAGGGGTTTAGCTCC+TGG + chr6.3:64197931-64197950 None:intergenic 65.0%
ACAGGCCCTGGCCTAACCCC+TGG + chr6.3:64197892-64197911 None:intergenic 70.0%
GTTAGGCCAGGGCCTGTGCC+AGG - chr6.3:64197895-64197914 MS.gene068430:intron 70.0%
TGCGCTCCTGGCACAGGCCC+TGG + chr6.3:64197904-64197923 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 64196989 64199599 64196989 ID=MS.gene068430
chr6.3 mRNA 64196989 64199599 64196989 ID=MS.gene068430.t1;Parent=MS.gene068430
chr6.3 exon 64199579 64199599 64199579 ID=MS.gene068430.t1.exon1;Parent=MS.gene068430.t1
chr6.3 CDS 64199579 64199599 64199579 ID=cds.MS.gene068430.t1;Parent=MS.gene068430.t1
chr6.3 exon 64199337 64199474 64199337 ID=MS.gene068430.t1.exon2;Parent=MS.gene068430.t1
chr6.3 CDS 64199337 64199474 64199337 ID=cds.MS.gene068430.t1;Parent=MS.gene068430.t1
chr6.3 exon 64198118 64198397 64198118 ID=MS.gene068430.t1.exon3;Parent=MS.gene068430.t1
chr6.3 CDS 64198118 64198397 64198118 ID=cds.MS.gene068430.t1;Parent=MS.gene068430.t1
chr6.3 exon 64197266 64197520 64197266 ID=MS.gene068430.t1.exon4;Parent=MS.gene068430.t1
chr6.3 CDS 64197266 64197520 64197266 ID=cds.MS.gene068430.t1;Parent=MS.gene068430.t1
chr6.3 exon 64197148 64197180 64197148 ID=MS.gene068430.t1.exon5;Parent=MS.gene068430.t1
chr6.3 CDS 64197148 64197180 64197148 ID=cds.MS.gene068430.t1;Parent=MS.gene068430.t1
chr6.3 exon 64196989 64197044 64196989 ID=MS.gene068430.t1.exon6;Parent=MS.gene068430.t1
chr6.3 CDS 64196989 64197044 64196989 ID=cds.MS.gene068430.t1;Parent=MS.gene068430.t1
Gene Sequence

>MS.gene068430

ATGTTTCAGAAGCTCCTACGTGTAAGGAGCATATATTCTTACTTTTCATTTTAAATTTTCATGACTGAAACATTGTGAATTGAGATATGCTACTAACTTGGTTGGTTTTTTTAATCTTTTTGAAGGGTCTAAGTTATCTGCACGGAGTCAGATATTTAGTTCACAGAGACATAAAGCCTGCTAATTTACTTGTAAACCTCAAAGGGGAGCCGAAAATTACTGATTTTGGTATTAGTGTTGGCATAGAGAATTCAGTTGCAATGGTCAGTCACAGTTTGTTGCTGTCATGCTTTGCTTCAACATACCGATTTGCTTCCACACTTTGTTTGCTTGACTGATGTGTATGTTTATAAGAAAATTCGAGTATGAGTTATTGCATTAAGTATTAGACTATGCTGTTAACGAATTATGAATTGTGTTTGAATTGTTTAGAAATGTTTGAATTGTGTATGTTTGAATTGTTTTGCTCCTATAGATAGAAATTGCACTTCAAAAGATGAGCAAGTCCGTATTGAGGATCTTGTTGAGCTGTGAGAGAACGACTAGATTATCTCATTGTTGAGAATGTATATGTTTCTTATCAATTTTTATCAATGTACTGTATTAAGTATTAATTTTGGGCCATAAAAATGGCTACTATCTATCTAATAATCATTCCCTAAAAGAAATATCTATCTAATTAAAAAAATTAAATTGTTTTTTAAAATTATTTTAATTATTAAATTTTAATATTTTGATTTTTTAAACAATTAAAAAACATTTTTTTTTAAAAATTCGTACACATTTTGCCAGGGGTTAACCCCTGGCAATGCTCCTGGCATGTTTTGAAAATGACTGCTGACACAGTCTGCAAAAGCCTGCACAATGCCAGAAAAGCTGCATGCAAGCTGACACAATTTGCCAGGGGTTAGGCCAGGGCCTGTGCCAGGAGCGCATTTTCCAGGAGCTAAACCCCTGGCAGGACAATTTGCGATGAGCAGATTTGCTGGTGATTCTGCTCCTGGCAAAACCCCTGGCAAACGTCCAGTTGCCAAGGCTTTTAGCATTTTACCAGGGCTTCTGCCCCTGGCAAAATGCAACATTTCTTGTAGTGAATGTAGACTCATTTTGCTAAATGCAGTCCATAGGTTGATCCATAACAACAATTAAGTGTCATTTATCAATTTATTCTAAAATCATATGTATGTTTTGAATACATACAGGAACCTGATATGAATACTGCGGCACAAATTGATAAACTAAAAAAGGAGGTTAGACAAATGCTTGTCTCAAAAACAGAGAAACCCATGGAAAAAGTCAACTTGATTGATTCAATATGTCGTTTGGGTGTGAGCTATCGTTTTGAAGACGAAATTAATCAAGTTTTGGAACATATTTACAAGAGTTATGTTCAAAATGGAGAAATAACTCTCAAAGACAACCTTCATTCTCTTGCTGTGCTATTTAGGGTGTTGAGGCAACATGGACTTTACGTTTCACCTGGTATATATATCTCAACTTATTCACATTAATGATTCATTTTTTATGTTTTTAATTTGGATCGCAAATTGAGTAAAACAAATTAGATTGGTAAACATTAAAAAAAAACTAATTGTAAAATCAATACATAAATTAACAACGTAATATTTGACTTTGTCATGGGTGTGTCTAATATGAATGTCTAAAAAATGGCATACCACTGTTAGCCTGTAAATCTTTTAGTAAAAAGAGAATTTTATAATAATAAAAGATAGAAAATAATATTATTTTTTTACATAAAATAGGAGTGTCTTTCGTTTGATCGGATACATATTAGACAACTCCTTATCTGTGTAATGTTCATATCCTCATTTCTTTTAATTATATTTTTTTTATATCATAGAAATAGACACTTAATGATTATAATGTAATATTAATGTATACATGCAGGATCTTATTAAGTAAGAACCTTTTTCGTACTTCAGAAACACACTATACATTATCTAGATTGCATAATGAATTTTTCAATCTTGCTTTAGCTTCTTATTTATATATATTTATAAATTAATATGTCTCTAATTGTTGTTTAATATTATTGTGGTTGTCTAATAAAAATACTAGATGTGTTCAACCAATTCAAGGACGAGGAAGGAAACTTCAGTGAAACATTGGTCAGAGATGTTGAGGGCATGTTAAACTTGTTTGAAGCATCACATATGATGGTTCATGGTGAAAAAATATTGGAAGAGGCGCTGGCTTTTACTTCTGGTCACCTTGAGTCCATGGCCACCCGATTGAGTCCTTCTCTTGCAACACAAGTAAAACAAAGCTTAAGACAATCTCTGCGGAAAAACTTGCCTAGGCTAGTGGCAAGAAGTTACATTTCCATCTATGAGCAAGATTCATCCCATAATGAAATTTTACTTGCATTGGCAAAATTGGATTTTAATATGCTCCAAAGCCTACATCAAAAGGAATTTGGAAACATTAGCAAGTAAGTACATTTTGAGCCTACATCAAAATATAAAAGTATATTAAATTGAGTTCAAAAAATAATTATACTAATTTCCTATACTATTTTTTGTCTTTATATATAGGTGGTGGAAGGAGTTGGATATTGCTCAAAAACTACCGTATGCACAAGATAGAGTAG
Protein sequence

>MS.gene068430.t1

MFQKLLRGLSYLHGVRYLVHRDIKPANLLVNLKGEPKITDFGISVGIENSVAMEPDMNTAAQIDKLKKEVRQMLVSKTEKPMEKVNLIDSICRLGVSYRFEDEINQVLEHIYKSYVQNGEITLKDNLHSLAVLFRVLRQHGLYVSPDVFNQFKDEEGNFSETLVRDVEGMLNLFEASHMMVHGEKILEEALAFTSGHLESMATRLSPSLATQVKQSLRQSLRKNLPRLVARTYIKRNLETLASGGRSWILLKNYRMHKIE