AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068501

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068501.t1 MTR_6g017010 65.667 300 57 5 98 355 474 769 1.18e-124 376
MS.gene068501.t1 MTR_5g085310 68.551 283 47 5 98 339 744 1025 1.02e-121 375
MS.gene068501.t1 MTR_6g017130 57.225 346 101 7 10 337 74 390 1.15e-114 338
MS.gene068501.t1 MTR_6g017130 82.474 97 17 0 1 97 1 97 1.08e-45 160
MS.gene068501.t1 MTR_0299s0030 65.779 263 47 6 1 260 86 308 1.53e-103 308
MS.gene068501.t1 MTR_0299s0030 66.667 48 15 1 308 355 298 344 7.87e-11 63.2
MS.gene068501.t1 MTR_5g017020 51.071 280 96 6 98 355 300 560 2.75e-74 240
MS.gene068501.t1 MTR_3g107960 69.136 162 43 3 98 252 860 1021 8.58e-67 228
MS.gene068501.t1 MTR_3g107960 74.444 90 22 1 266 355 1118 1206 1.95e-37 143
MS.gene068501.t1 MTR_5g017020 54.018 224 79 5 98 297 300 523 9.32e-64 211
MS.gene068501.t1 MTR_3g101100 64.881 168 46 4 98 252 1035 1202 2.58e-61 213
MS.gene068501.t1 MTR_3g101100 71.111 90 25 1 266 355 1307 1395 1.90e-34 134
MS.gene068501.t1 MTR_6g017000 58.025 162 61 3 98 252 778 939 1.07e-51 184
MS.gene068501.t1 MTR_6g017140 62.745 102 36 1 124 223 347 448 2.83e-35 134
MS.gene068501.t1 MTR_1g047940 52.349 149 46 5 199 345 73 198 8.38e-35 127
MS.gene068501.t1 MTR_4g005050 39.716 141 76 2 121 252 1385 1525 8.71e-28 115
MS.gene068501.t1 MTR_4g005050 50.000 82 40 1 266 347 1615 1695 2.14e-20 93.2
MS.gene068501.t1 MTR_5g015680 43.243 74 40 2 258 329 174 247 2.77e-13 69.7
MS.gene068501.t1 MTR_5g015680 43.243 74 40 2 258 329 269 342 4.22e-13 70.1
MS.gene068501.t1 MTR_5g015680 43.243 74 40 2 258 329 308 381 5.72e-13 70.1
MS.gene068501.t1 MTR_6g017120 58.462 65 21 2 63 127 94 152 2.09e-11 62.0
MS.gene068501.t1 MTR_5g085317 77.778 36 8 0 98 133 59 94 6.80e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068501.t1 AT3G12980 30.124 322 142 7 108 347 1335 1655 7.20e-41 153
MS.gene068501.t1 AT3G12980 30.124 322 142 7 108 347 1254 1574 1.05e-40 153
MS.gene068501.t1 AT1G16710 38.323 167 89 4 100 252 1326 1492 3.22e-31 125
MS.gene068501.t1 AT1G16710 51.899 79 37 1 269 347 1586 1663 4.00e-21 95.5
MS.gene068501.t1 AT1G16710 38.323 167 89 4 100 252 1290 1456 3.27e-31 125
MS.gene068501.t1 AT1G16710 51.899 79 37 1 269 347 1550 1627 4.14e-21 95.5
MS.gene068501.t1 AT1G16710 38.323 167 89 4 100 252 1290 1456 3.27e-31 125
MS.gene068501.t1 AT1G16710 51.899 79 37 1 269 347 1550 1627 4.14e-21 95.5
MS.gene068501.t1 AT1G16710 38.323 167 89 4 100 252 1290 1456 3.27e-31 125
MS.gene068501.t1 AT1G16710 51.899 79 37 1 269 347 1550 1627 4.14e-21 95.5
MS.gene068501.t1 AT1G16710 38.323 167 89 4 100 252 1329 1495 3.50e-31 125
MS.gene068501.t1 AT1G16710 51.899 79 37 1 269 347 1589 1666 4.15e-21 95.5
MS.gene068501.t1 AT1G16710 38.323 167 89 4 100 252 1329 1495 3.50e-31 125
MS.gene068501.t1 AT1G16710 51.899 79 37 1 269 347 1589 1666 4.15e-21 95.5
MS.gene068501.t1 AT1G16710 38.323 167 89 4 100 252 1329 1495 3.50e-31 125
MS.gene068501.t1 AT1G16710 51.899 79 37 1 269 347 1589 1666 4.15e-21 95.5
MS.gene068501.t1 AT1G16710 38.323 167 89 4 100 252 1329 1495 3.50e-31 125
MS.gene068501.t1 AT1G16710 51.899 79 37 1 269 347 1589 1666 4.15e-21 95.5
MS.gene068501.t1 AT1G16710 38.323 167 89 4 100 252 1355 1521 3.75e-31 125
MS.gene068501.t1 AT1G16710 51.899 79 37 1 269 347 1615 1692 4.05e-21 95.5
MS.gene068501.t1 AT1G79000 37.126 167 91 4 100 252 1346 1512 4.87e-30 122
MS.gene068501.t1 AT1G79000 50.000 82 40 1 266 347 1603 1683 2.59e-21 96.3
MS.gene068501.t1 AT1G79000 37.126 167 91 4 100 252 1346 1512 5.13e-30 122
MS.gene068501.t1 AT1G79000 50.000 82 40 1 266 347 1603 1683 2.82e-21 95.9
MS.gene068501.t1 AT1G79000 37.126 167 91 4 100 252 1322 1488 5.21e-30 122
MS.gene068501.t1 AT1G79000 50.000 82 40 1 266 347 1579 1659 2.89e-21 95.9
MS.gene068501.t1 AT1G55970 39.241 158 83 3 108 252 1117 1274 2.41e-27 114
MS.gene068501.t1 AT1G55970 39.241 158 83 3 108 252 1131 1288 2.50e-27 114
MS.gene068501.t1 AT1G67220 34.302 172 96 4 98 252 1037 1208 3.84e-21 95.5
MS.gene068501.t1 AT1G67220 33.333 102 64 2 235 332 1265 1366 8.64e-11 63.9
MS.gene068501.t1 AT1G16710 36.264 91 51 3 100 183 1329 1419 6.68e-12 67.4
MS.gene068501.t1 AT5G67480 40.000 85 47 3 258 339 273 356 1.05e-11 65.9
MS.gene068501.t1 AT5G67480 40.000 85 47 3 258 339 262 345 1.09e-11 65.9
MS.gene068501.t1 AT5G67480 40.000 85 47 3 258 339 262 345 1.09e-11 65.9

Find 59 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTGTAGGCAATATATTT+TGG 0.161705 4.2:-58038929 None:intergenic
AGAGTGGATTGTTTGAAAAT+AGG 0.213298 4.2:+58039345 MS.gene068501:CDS
GCTCTCCAATCTTACGGTTT+TGG 0.244128 4.2:-58037662 None:intergenic
GAGTGGATTGTTTGAAAATA+GGG 0.257007 4.2:+58039346 MS.gene068501:CDS
TTCCGAGTCTTTGCAATTCC+TGG 0.258036 4.2:-58039812 None:intergenic
TCGGGATTTGAAAGCGATTC+GGG 0.305182 4.2:+58037833 MS.gene068501:CDS
TTGCATCACCAGAATGTCTT+TGG 0.306980 4.2:-58038806 None:intergenic
AATGTAGAATACCGCGTTGC+AGG 0.320641 4.2:+58039833 MS.gene068501:CDS
CAAGTCTTCCAAAGACATTC+TGG 0.349204 4.2:+58038798 MS.gene068501:CDS
TTGTCGTAGAGCTGATGTTT+TGG 0.370193 4.2:+58040038 MS.gene068501:CDS
AAATAGAACAGTAAAGGAAT+TGG 0.375938 4.2:+58038768 MS.gene068501:CDS
CCGAAAATTAGATACAATAT+TGG 0.377417 4.2:+58037894 MS.gene068501:CDS
CAGAATGTCTTTGGAAGACT+TGG 0.402689 4.2:-58038797 None:intergenic
AAAGTTGGGAAGACATCAAT+TGG 0.408372 4.2:+58037778 MS.gene068501:CDS
TTATACTAACTACGCATTCC+AGG 0.411495 4.2:+58039794 MS.gene068501:CDS
GTCGGGATTTGAAAGCGATT+CGG 0.419586 4.2:+58037832 MS.gene068501:CDS
TGGGAAGCTCTCCAATCTTA+CGG 0.420073 4.2:-58037668 None:intergenic
TTCTTGATATGTGAACAGTT+TGG 0.436055 4.2:-58039700 None:intergenic
AAGTGCAAAATTCGAGTGAA+TGG 0.447991 4.2:+58039742 MS.gene068501:CDS
AATAGAACAGTAAAGGAATT+GGG 0.448928 4.2:+58038769 MS.gene068501:CDS
GAAAGCGACGGAGCAGTCAT+TGG 0.464537 4.2:+58040060 MS.gene068501:CDS
CAAACTGAAACTGAGACTTC+TGG 0.468204 4.2:-58039388 None:intergenic
GTTCATCATGGAGGAATACT+TGG 0.469438 4.2:-58039426 None:intergenic
ATCGCTTCCTAGTCACTTCA+CGG 0.477927 4.2:-58038995 None:intergenic
TGAGGTGCATACACTTTGTC+AGG 0.483949 4.2:+58039184 MS.gene068501:CDS
GACAAACACACTTCAAAGAA+TGG 0.487190 4.2:+58039161 MS.gene068501:CDS
AGTGCAAAATTCGAGTGAAT+GGG 0.490592 4.2:+58039743 MS.gene068501:CDS
TTCTGGCACGGAGTAAATAG+TGG 0.495651 4.2:-58039451 None:intergenic
GTGTCCCAAAACCGTAAGAT+TGG 0.496680 4.2:+58037657 MS.gene068501:CDS
TTCACATATTTCAGTTGCCA+TGG 0.496893 4.2:+58039134 MS.gene068501:intron
TGGGACACCGCATTGTTAGT+TGG 0.503360 4.2:-58037642 None:intergenic
TTTGAAAGCGATTCGGGACA+AGG 0.523517 4.2:+58037839 MS.gene068501:CDS
AGTGTCAAATAGAACAGTAA+AGG 0.526544 4.2:+58038762 MS.gene068501:CDS
TAAATAGTGGAGGTTCATCA+TGG 0.529805 4.2:-58039438 None:intergenic
ATCATTGTGTTTAGTGTCGG+AGG 0.535711 4.2:-58039312 None:intergenic
AACAGTAAAGGAATTGGGAC+AGG 0.537323 4.2:+58038774 MS.gene068501:CDS
GATATCATTGTGTTTAGTGT+CGG 0.557814 4.2:-58039315 None:intergenic
TTTCAAACAATCCACTCTCA+AGG 0.566291 4.2:-58039340 None:intergenic
AAGCGATTCGGGACAAGGCA+AGG 0.568591 4.2:+58037844 MS.gene068501:CDS
TTCCAGGAATTGCAAAGACT+CGG 0.570649 4.2:+58039810 MS.gene068501:CDS
TCATTGTGTTTAGTGTCGGA+GGG 0.572940 4.2:-58039311 None:intergenic
AGAAGAAGCATGTAGTAGAA+CGG 0.584747 4.2:+58039995 MS.gene068501:CDS
AATGATATCGTCCTTGAGAG+TGG 0.593789 4.2:+58039329 MS.gene068501:CDS
GATTTGTTAAAAGAGAGTAG+AGG 0.596875 4.2:+58038715 MS.gene068501:intron
GTGCAAAATTCGAGTGAATG+GGG 0.599551 4.2:+58039744 MS.gene068501:CDS
CATTGTGTTTAGTGTCGGAG+GGG 0.617022 4.2:-58039310 None:intergenic
GTGAAGTGACTAGGAAGCGA+TGG 0.621910 4.2:+58038997 MS.gene068501:CDS
GATACAATACTGGTAAGCGT+TGG 0.634031 4.2:+58037712 MS.gene068501:CDS
TGCAAAATTCGAGTGAATGG+GGG 0.636265 4.2:+58039745 MS.gene068501:CDS
TGCACTGCCGTGAAGTGACT+AGG 0.638015 4.2:+58038988 MS.gene068501:CDS
TGAAAATGAGACTTCTGGCA+CGG 0.640154 4.2:-58039463 None:intergenic
TAAGATTGGAGAGCTTCCCA+AGG 0.665492 4.2:+58037671 MS.gene068501:CDS
TGGAGGAATACTTGGCACGA+CGG 0.668087 4.2:-58039418 None:intergenic
AAGTGTGTTTGTCAGAACCA+TGG 0.669857 4.2:-58039151 None:intergenic
TGGCACGGAGTAAATAGTGG+AGG 0.693396 4.2:-58039448 None:intergenic
ACACACTTCAAAGAATGGTG+AGG 0.696219 4.2:+58039166 MS.gene068501:CDS
CAGTGATCCAACTAACAATG+CGG 0.745719 4.2:+58037635 MS.gene068501:CDS
ATAGTGGAGGTTCATCATGG+AGG 0.747699 4.2:-58039435 None:intergenic
ATTGTAACACACCTGCAACG+CGG 0.761234 4.2:-58039844 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTCATAATTTTGATT+AGG + chr4.2:58038902-58038921 MS.gene068501:intron 10.0%
!! AAAATTATATGAAAAACATG+AGG - chr4.2:58039222-58039241 None:intergenic 15.0%
!! ACAATAAGAAAATACTTTAT+TGG - chr4.2:58038401-58038420 None:intergenic 15.0%
!! GAAAATAACTTACATTATAT+TGG - chr4.2:58038890-58038909 None:intergenic 15.0%
!! ATCATGTTGTGAATTTATTA+AGG - chr4.2:58038118-58038137 None:intergenic 20.0%
!!! AGCATTGAATGAAATAAAAA+GGG + chr4.2:58039091-58039110 MS.gene068501:intron 20.0%
!!! ATGAAGAAAAATTGTTTTGT+CGG + chr4.2:58037814-58037833 MS.gene068501:CDS 20.0%
!!! ATTATATTGGCCTATAAAAA+AGG - chr4.2:58038877-58038896 None:intergenic 20.0%
!!! CATATAATTTTCTCATCATT+AGG + chr4.2:58039231-58039250 MS.gene068501:intron 20.0%
!!! CCAATATTGTATCTAATTTT+CGG - chr4.2:58037897-58037916 None:intergenic 20.0%
!!! CTTGAAGATTTCATTTTATT+AGG - chr4.2:58038243-58038262 None:intergenic 20.0%
!!! TAGCATTGAATGAAATAAAA+AGG + chr4.2:58039090-58039109 MS.gene068501:intron 20.0%
!!! TTTCATAATTTTGATTAGGT+TGG + chr4.2:58038906-58038925 MS.gene068501:intron 20.0%
!!! TTTTAAATCTTTCAACTCAT+TGG + chr4.2:58038444-58038463 MS.gene068501:intron 20.0%
! AAAAATTTCAGGATTGTGAA+AGG + chr4.2:58039036-58039055 MS.gene068501:CDS 25.0%
! AAATAGAACAGTAAAGGAAT+TGG + chr4.2:58038768-58038787 MS.gene068501:CDS 25.0%
! AAGGAAGTTAATCCAAAAAT+TGG + chr4.2:58037690-58037709 MS.gene068501:CDS 25.0%
! AATAGAACAGTAAAGGAATT+GGG + chr4.2:58038769-58038788 MS.gene068501:CDS 25.0%
! ATATACAAGAACAATGGTTA+AGG - chr4.2:58038183-58038202 None:intergenic 25.0%
! ATATGAAAAACATGAGGTTT+AGG - chr4.2:58039216-58039235 None:intergenic 25.0%
! ATGAAGAAAAACTGTTTGAT+CGG + chr4.2:58038006-58038025 MS.gene068501:intron 25.0%
! CCGAAAATTAGATACAATAT+TGG + chr4.2:58037894-58037913 MS.gene068501:CDS 25.0%
! GTAGAATATCAAAATTCTCT+AGG - chr4.2:58039885-58039904 None:intergenic 25.0%
! GTTGTGAATTTATTAAGGAA+GGG - chr4.2:58038113-58038132 None:intergenic 25.0%
! TACAACATATACAAGAACAA+TGG - chr4.2:58038189-58038208 None:intergenic 25.0%
! TATACAAGAACAATGGTTAA+GGG - chr4.2:58038182-58038201 None:intergenic 25.0%
! TGTTGTGAATTTATTAAGGA+AGG - chr4.2:58038114-58038133 None:intergenic 25.0%
!! GATTCAATAAGTTCTTTTCT+GGG + chr4.2:58038502-58038521 MS.gene068501:intron 25.0%
!! TGATTCAATAAGTTCTTTTC+TGG + chr4.2:58038501-58038520 MS.gene068501:intron 25.0%
!!! AATTTTTGGATTAACTTCCT+TGG - chr4.2:58037691-58037710 None:intergenic 25.0%
!!! ATTTTTGGATTAACTTCCTT+GGG - chr4.2:58037690-58037709 None:intergenic 25.0%
!!! TCTTCTACATCCTTTTTTAT+AGG + chr4.2:58038864-58038883 MS.gene068501:intron 25.0%
!!! TGAAGAAAAATTGTTTTGTC+GGG + chr4.2:58037815-58037834 MS.gene068501:CDS 25.0%
AAAGTTGGAAAGACATCAAT+TGG + chr4.2:58037970-58037989 MS.gene068501:intron 30.0%
ACATCAATTGGAAAGACATA+AGG + chr4.2:58037982-58038001 MS.gene068501:intron 30.0%
AGTGTCAAATAGAACAGTAA+AGG + chr4.2:58038762-58038781 MS.gene068501:CDS 30.0%
CATCAATTGGAAAGACATAA+GGG + chr4.2:58037983-58038002 MS.gene068501:intron 30.0%
CCAAAAATTGGATACAATAC+TGG + chr4.2:58037702-58037721 MS.gene068501:CDS 30.0%
CGTCTTAGATACAAAAAGTT+GGG + chr4.2:58037764-58037783 MS.gene068501:CDS 30.0%
GATATCATTGTGTTTAGTGT+CGG - chr4.2:58039318-58039337 None:intergenic 30.0%
TGAAGAAAAACTGTTTGATC+GGG + chr4.2:58038007-58038026 MS.gene068501:intron 30.0%
TTCTTGATATGTGAACAGTT+TGG - chr4.2:58039703-58039722 None:intergenic 30.0%
! ACCATATCTTTTTACAATGC+TGG - chr4.2:58039774-58039793 None:intergenic 30.0%
! ACGATTAAGTTTTCTCTTGT+AGG - chr4.2:58038945-58038964 None:intergenic 30.0%
! CCAACTTTTTGTATCTAAGA+CGG - chr4.2:58037766-58037785 None:intergenic 30.0%
! CCAGTATTGTATCCAATTTT+TGG - chr4.2:58037705-58037724 None:intergenic 30.0%
! CTCTTGTAGGCAATATATTT+TGG - chr4.2:58038932-58038951 None:intergenic 30.0%
!! AGAGTGGATTGTTTGAAAAT+AGG + chr4.2:58039345-58039364 MS.gene068501:CDS 30.0%
!! GAGTGGATTGTTTGAAAATA+GGG + chr4.2:58039346-58039365 MS.gene068501:CDS 30.0%
!! GATTTGTTAAAAGAGAGTAG+AGG + chr4.2:58038715-58038734 MS.gene068501:intron 30.0%
!! GGCAAGGAAACTTTTTAAAA+AGG + chr4.2:58037860-58037879 MS.gene068501:CDS 30.0%
!! TCTGAGATTGAAAATTTTCC+TGG - chr4.2:58038057-58038076 None:intergenic 30.0%
!! TTGAACACTTTTGAGTCAAT+TGG - chr4.2:58039504-58039523 None:intergenic 30.0%
!!! AAAACTTATTTTGTGCCTGA+AGG - chr4.2:58038087-58038106 None:intergenic 30.0%
!!! TTGAATTATGTGCCATTTTC+AGG + chr4.2:58039271-58039290 MS.gene068501:intron 30.0%
AAAACTGTTTGATCGGGATT+TGG + chr4.2:58038013-58038032 MS.gene068501:intron 35.0%
AAAGTTGGGAAGACATCAAT+TGG + chr4.2:58037778-58037797 MS.gene068501:CDS 35.0%
AAGAACAATGGTTAAGGGAT+TGG - chr4.2:58038177-58038196 None:intergenic 35.0%
AAGATTTAGAGAGTGAGTCA+GGG - chr4.2:58038648-58038667 None:intergenic 35.0%
AAGTGCAAAATTCGAGTGAA+TGG + chr4.2:58039742-58039761 MS.gene068501:CDS 35.0%
AGTGCAAAATTCGAGTGAAT+GGG + chr4.2:58039743-58039762 MS.gene068501:CDS 35.0%
ATCTAACAAGTTCAGAAGAC+AGG - chr4.2:58038698-58038717 None:intergenic 35.0%
CACTGAATGCGAAAAATTTC+AGG + chr4.2:58039025-58039044 MS.gene068501:CDS 35.0%
CCGTCTTAGATACAAAAAGT+TGG + chr4.2:58037763-58037782 MS.gene068501:CDS 35.0%
CCTTCTTAGATGCAAAAAGT+TGG + chr4.2:58037955-58037974 MS.gene068501:intron 35.0%
GAAACTGAAAATGAGACTTC+TGG - chr4.2:58039471-58039490 None:intergenic 35.0%
GACAAACACACTTCAAAGAA+TGG + chr4.2:58039161-58039180 MS.gene068501:CDS 35.0%
GATACAATATTGGTAAGCGA+TGG + chr4.2:58037904-58037923 MS.gene068501:intron 35.0%
GCCAGCATTGTAAAAAGATA+TGG + chr4.2:58039770-58039789 MS.gene068501:CDS 35.0%
GGCAACTGAAATATGTGAAA+TGG - chr4.2:58039133-58039152 None:intergenic 35.0%
TCAGGAGTCAAATAAGGAAA+TGG + chr4.2:58039546-58039565 MS.gene068501:intron 35.0%
TTATACTAACTACGCATTCC+AGG + chr4.2:58039794-58039813 MS.gene068501:CDS 35.0%
TTCACATATTTCAGTTGCCA+TGG + chr4.2:58039134-58039153 MS.gene068501:intron 35.0%
TTTCAAACAATCCACTCTCA+AGG - chr4.2:58039343-58039362 None:intergenic 35.0%
! AAGCTTCAAGAAAAGTGTGA+AGG - chr4.2:58038294-58038313 None:intergenic 35.0%
! AGAAGAAGCATGTAGTAGAA+CGG + chr4.2:58039995-58040014 MS.gene068501:CDS 35.0%
! AGACTCTGAAGATGAAAATG+AGG - chr4.2:58038542-58038561 None:intergenic 35.0%
! CCAACTTTTTGCATCTAAGA+AGG - chr4.2:58037958-58037977 None:intergenic 35.0%
! TAAATAGTGGAGGTTCATCA+TGG - chr4.2:58039441-58039460 None:intergenic 35.0%
! TCATTTTCATCTTCAGAGTC+TGG + chr4.2:58038541-58038560 MS.gene068501:intron 35.0%
AACAGTAAAGGAATTGGGAC+AGG + chr4.2:58038774-58038793 MS.gene068501:CDS 40.0%
AAGGGATTGGATTCTCAGAA+GGG - chr4.2:58038164-58038183 None:intergenic 40.0%
AAGTGTGTTTGTCAGAACCA+TGG - chr4.2:58039154-58039173 None:intergenic 40.0%
AATGATATCGTCCTTGAGAG+TGG + chr4.2:58039329-58039348 MS.gene068501:CDS 40.0%
ACACACTTCAAAGAATGGTG+AGG + chr4.2:58039166-58039185 MS.gene068501:CDS 40.0%
AGTATGTCAATCTCGGAATC+AGG + chr4.2:58039528-58039547 MS.gene068501:intron 40.0%
ATGAAGCAAATGTGGTTGTG+GGG - chr4.2:58038603-58038622 None:intergenic 40.0%
CAAACTGAAACTGAGACTTC+TGG - chr4.2:58039391-58039410 None:intergenic 40.0%
CAACACCAGTATGTCAATCT+CGG + chr4.2:58039521-58039540 MS.gene068501:intron 40.0%
CAAGTCTTCCAAAGACATTC+TGG + chr4.2:58038798-58038817 MS.gene068501:CDS 40.0%
CAGTGATCCAACTAACAATG+CGG + chr4.2:58037635-58037654 MS.gene068501:CDS 40.0%
CATCAACAGCAACCTGAAAA+TGG - chr4.2:58039286-58039305 None:intergenic 40.0%
GAAGATTTAGAGAGTGAGTC+AGG - chr4.2:58038649-58038668 None:intergenic 40.0%
GATACAATACTGGTAAGCGT+TGG + chr4.2:58037712-58037731 MS.gene068501:CDS 40.0%
GATGAAGCAAATGTGGTTGT+GGG - chr4.2:58038604-58038623 None:intergenic 40.0%
GTGCAAAATTCGAGTGAATG+GGG + chr4.2:58039744-58039763 MS.gene068501:CDS 40.0%
GTTCATCATGGAGGAATACT+TGG - chr4.2:58039429-58039448 None:intergenic 40.0%
TAAGGGATTGGATTCTCAGA+AGG - chr4.2:58038165-58038184 None:intergenic 40.0%
TCGGAATCAGGAGTCAAATA+AGG + chr4.2:58039540-58039559 MS.gene068501:intron 40.0%
TGAAAATGAGACTTCTGGCA+CGG - chr4.2:58039466-58039485 None:intergenic 40.0%
TGATTCCGAGATTGACATAC+TGG - chr4.2:58039529-58039548 None:intergenic 40.0%
TGCAAAATTCGAGTGAATGG+GGG + chr4.2:58039745-58039764 MS.gene068501:CDS 40.0%
TTCCAGGAATTGCAAAGACT+CGG + chr4.2:58039810-58039829 MS.gene068501:CDS 40.0%
TTGCATCACCAGAATGTCTT+TGG - chr4.2:58038809-58038828 None:intergenic 40.0%
! ATCATTGTGTTTAGTGTCGG+AGG - chr4.2:58039315-58039334 None:intergenic 40.0%
! ATCTGAAAAGCATGTGAGGA+AGG - chr4.2:58038378-58038397 None:intergenic 40.0%
! CACTCGAATTTTGCACTTAC+TGG - chr4.2:58039740-58039759 None:intergenic 40.0%
! CAGAATGTCTTTGGAAGACT+TGG - chr4.2:58038800-58038819 None:intergenic 40.0%
! CTCTCCAATCTTACGGTTTT+GGG - chr4.2:58037664-58037683 None:intergenic 40.0%
! TCATTGTGTTTAGTGTCGGA+GGG - chr4.2:58039314-58039333 None:intergenic 40.0%
! TTGGATCTGAAAAGCATGTG+AGG - chr4.2:58038382-58038401 None:intergenic 40.0%
! TTGTCGTAGAGCTGATGTTT+TGG + chr4.2:58040038-58040057 MS.gene068501:CDS 40.0%
! TTTGGATGCGATTTAGGACA+AGG + chr4.2:58038031-58038050 MS.gene068501:intron 40.0%
!! CTTACTGACTCATACACAGA+TGG + chr4.2:58039637-58039656 MS.gene068501:intron 40.0%
AATGTAGAATACCGCGTTGC+AGG + chr4.2:58039833-58039852 MS.gene068501:CDS 45.0%
ACAGGTCAAGCAGAATCTGA+TGG - chr4.2:58038680-58038699 None:intergenic 45.0%
ACCACATTTGCTTCATCCTC+AGG + chr4.2:58038607-58038626 MS.gene068501:intron 45.0%
ACTCTCTAAATCTTCCCAGG+AGG + chr4.2:58038654-58038673 MS.gene068501:intron 45.0%
ATCGCTTCCTAGTCACTTCA+CGG - chr4.2:58038998-58039017 None:intergenic 45.0%
ATCTCAGACTCAACACCTTC+AGG + chr4.2:58038069-58038088 MS.gene068501:intron 45.0%
ATTGTAACACACCTGCAACG+CGG - chr4.2:58039847-58039866 None:intergenic 45.0%
CTCACTCTCTAAATCTTCCC+AGG + chr4.2:58038651-58038670 MS.gene068501:intron 45.0%
GGATGAAGCAAATGTGGTTG+TGG - chr4.2:58038605-58038624 None:intergenic 45.0%
GTCGGGATTTGAAAGCGATT+CGG + chr4.2:58037832-58037851 MS.gene068501:CDS 45.0%
GTCTGATAAGGATGATCCTG+AGG - chr4.2:58038626-58038645 None:intergenic 45.0%
GTGTCCCAAAACCGTAAGAT+TGG + chr4.2:58037657-58037676 MS.gene068501:CDS 45.0%
TAAGATTGGAGAGCTTCCCA+AGG + chr4.2:58037671-58037690 MS.gene068501:CDS 45.0%
TCCTGAGGATGAAGCAAATG+TGG - chr4.2:58038611-58038630 None:intergenic 45.0%
TCGGGATTTGAAAGCGATTC+GGG + chr4.2:58037833-58037852 MS.gene068501:CDS 45.0%
TCGGGATTTGGATGCGATTT+AGG + chr4.2:58038025-58038044 MS.gene068501:intron 45.0%
TGAGGTGCATACACTTTGTC+AGG + chr4.2:58039184-58039203 MS.gene068501:CDS 45.0%
TGGGAAGCTCTCCAATCTTA+CGG - chr4.2:58037671-58037690 None:intergenic 45.0%
TTCCGAGTCTTTGCAATTCC+TGG - chr4.2:58039815-58039834 None:intergenic 45.0%
TTCTGGCACGGAGTAAATAG+TGG - chr4.2:58039454-58039473 None:intergenic 45.0%
! ATAGTGGAGGTTCATCATGG+AGG - chr4.2:58039438-58039457 None:intergenic 45.0%
! CATTGTGTTTAGTGTCGGAG+GGG - chr4.2:58039313-58039332 None:intergenic 45.0%
! GCTCTCCAATCTTACGGTTT+TGG - chr4.2:58037665-58037684 None:intergenic 45.0%
! TCATCTTCAGAGTCTGGTTC+AGG + chr4.2:58038547-58038566 MS.gene068501:intron 45.0%
! TTTGAAAGCGATTCGGGACA+AGG + chr4.2:58037839-58037858 MS.gene068501:CDS 45.0%
!!! GCTGATGTTTTGGAAAGCGA+CGG + chr4.2:58040048-58040067 MS.gene068501:CDS 45.0%
GAGTGAGTCAGGGTCTGATA+AGG - chr4.2:58038638-58038657 None:intergenic 50.0%
GTGAAGTGACTAGGAAGCGA+TGG + chr4.2:58038997-58039016 MS.gene068501:CDS 50.0%
TGGAGGAATACTTGGCACGA+CGG - chr4.2:58039421-58039440 None:intergenic 50.0%
TGGCACGGAGTAAATAGTGG+AGG - chr4.2:58039451-58039470 None:intergenic 50.0%
TGGGACACCGCATTGTTAGT+TGG - chr4.2:58037645-58037664 None:intergenic 50.0%
! ATGCGATTTAGGACAAGGCC+AGG + chr4.2:58038036-58038055 MS.gene068501:intron 50.0%
! TGTGGGGTTAGTAGCTACAG+TGG - chr4.2:58038587-58038606 None:intergenic 50.0%
AGCAGAATCTGATGGCCTCC+TGG - chr4.2:58038672-58038691 None:intergenic 55.0%
GAAAGCGACGGAGCAGTCAT+TGG + chr4.2:58040060-58040079 MS.gene068501:CDS 55.0%
GCAGAATCTGATGGCCTCCT+GGG - chr4.2:58038671-58038690 None:intergenic 55.0%
GCATGTGAGGAAGGACTCAG+AGG - chr4.2:58038369-58038388 None:intergenic 55.0%
TGCACTGCCGTGAAGTGACT+AGG + chr4.2:58038988-58039007 MS.gene068501:CDS 55.0%
! AAGCGATTCGGGACAAGGCA+AGG + chr4.2:58037844-58037863 MS.gene068501:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 58037624 58040089 58037624 ID=MS.gene068501
chr4.2 mRNA 58037624 58040089 58037624 ID=MS.gene068501.t1;Parent=MS.gene068501
chr4.2 exon 58037624 58037915 58037624 ID=MS.gene068501.t1.exon1;Parent=MS.gene068501.t1
chr4.2 CDS 58037624 58037915 58037624 ID=cds.MS.gene068501.t1;Parent=MS.gene068501.t1
chr4.2 exon 58038728 58038831 58038728 ID=MS.gene068501.t1.exon2;Parent=MS.gene068501.t1
chr4.2 CDS 58038728 58038831 58038728 ID=cds.MS.gene068501.t1;Parent=MS.gene068501.t1
chr4.2 exon 58038924 58039057 58038924 ID=MS.gene068501.t1.exon3;Parent=MS.gene068501.t1
chr4.2 CDS 58038924 58039057 58038924 ID=cds.MS.gene068501.t1;Parent=MS.gene068501.t1
chr4.2 exon 58039148 58039205 58039148 ID=MS.gene068501.t1.exon4;Parent=MS.gene068501.t1
chr4.2 CDS 58039148 58039205 58039148 ID=cds.MS.gene068501.t1;Parent=MS.gene068501.t1
chr4.2 exon 58039293 58039473 58039293 ID=MS.gene068501.t1.exon5;Parent=MS.gene068501.t1
chr4.2 CDS 58039293 58039473 58039293 ID=cds.MS.gene068501.t1;Parent=MS.gene068501.t1
chr4.2 exon 58039656 58039854 58039656 ID=MS.gene068501.t1.exon6;Parent=MS.gene068501.t1
chr4.2 CDS 58039656 58039854 58039656 ID=cds.MS.gene068501.t1;Parent=MS.gene068501.t1
chr4.2 exon 58039990 58040089 58039990 ID=MS.gene068501.t1.exon7;Parent=MS.gene068501.t1
chr4.2 CDS 58039990 58040089 58039990 ID=cds.MS.gene068501.t1;Parent=MS.gene068501.t1
Gene Sequence

>MS.gene068501

ATGGCGGCAAACAGTGATCCAACTAACAATGCGGTGTCCCAAAACCGTAAGATTGGAGAGCTTCCCAAGGAAGTTAATCCAAAAATTGGATACAATACTGGTAAGCGTTGGTCAAATGATGAAAACAATGTGCTCAAGTCCGTCTTAGATACAAAAAGTTGGGAAGACATCAATTGGAAAGACATCAGTAATGAAGAAAAATTGTTTTGTCGGGATTTGAAAGCGATTCGGGACAAGGCAAGGAAACTTTTTAAAAAGGAAAGACAAGATCCGAAAATTAGATACAATATTGGTAAGCGATGGTCAGATGATGAAAACAATGCGCTCAAGTCCTTCTTAGATGCAAAAAGTTGGAAAGACATCAATTGGAAAGACATAAGGGATGAAGAAAAACTGTTTGATCGGGATTTGGATGCGATTTAGGACAAGGCCAGGAAAATTTTCAATCTCAGACTCAACACCTTCAGGCACAAAATAAGTTTTCAACCCTTCCTTAATAAATTCACAACATGATTTAAGATTGATTTCATTTGATTTCCCTTCTGAGAATCCAATCCCTTAACCATTGTTCTTGTATATGTTGTAAATCATAGAAGCTATTTTGCTTCTGTCAATTCCTAATAAAATGAAATCTTCAAGAGCAATATCTTGTTTACTTAAAAGTTTTCCTTCACACTTTTCTTGAAGCTTCTGACAAAGAAATTTGAGTTTGTCTTCATAAGTTTTACAAATGAAGTCAAAACCTCTGAGTCCTTCCTCACATGCTTTTCAGATCCAATAAAGTATTTTCTTATTGTTTCATTAAATCAACATATTTATCTTTTAAATCTTTCAACTCATTGGTTATGTGTTTAAAGTGTGTGTTGAGCTCTTTAAGTGATTCAATAAGTTCTTTTCTGGGAATTTTATAGTATACCTCATTTTCATCTTCAGAGTCTGGTTCAGGTTTAGCATCTGAAGCCACTGTAGCTACTAACCCCACAACCACATTTGCTTCATCCTCAGGATCATCCTTATCAGACCCTGACTCACTCTCTAAATCTTCCCAGGAGGCCATCAGATTCTGCTTGACCTGTCTTCTGAACTTGTTAGATTTGTTAAAAGAGAGTAGAGGAGACTATGAAAAGTTGTTAAAACAAGTGTCAAATAGAACAGTAAAGGAATTGGGACAGGCCAAGTCTTCCAAAGACATTCTGGTGATGCAAAAAGTATGTATATCTTCTCTTTTACTTTGTAGTGTTCTTCTACATCCTTTTTTATAGGCCAATATAATGTAAGTTATTTTCATAATTTTGATTAGGTTGGCCAAAATATATTGCCTACAAGAGAAAACTTAATCGTAGCTCATTTGCGATCTTCATGCATGCACTGCCGTGAAGTGACTAGGAAGCGATGGTTTTACACTGAATGCGAAAAATTTCAGGATTGTGAAAGGTATGATAACTTGAATACATATATAAAATGTTTAGCATTGAATGAAATAAAAAGGGATTTTTTCATTGACTTCCATTTCACATATTTCAGTTGCCATGGTTCTGACAAACACACTTCAAAGAATGGTGAGGTGCATACACTTTGTCAGGTAACAACCTAAACCTCATGTTTTTCATATAATTTTCTCATCATTAGGTAATTTCGAGCAAAACATTGAATTATGTGCCATTTTCAGGTTGCTGTTGATGATTTCCCCTCCGACACTAAACACAATGATATCGTCCTTGAGAGTGGATTGTTTGAAAATAGGGACAACTTTTTTATATTCTGCCAGAAGTCTCAGTTTCAGTTTGACACACTCCGTCGTGCCAAGTATTCCTCCATGATGAACCTCCACTATTTACTCCGTGCCAGAAGTCTCATTTTCAGTTTCAAAATGAACACCAATTGACTCAAAAGTGTTCAACACCAGTATGTCAATCTCGGAATCAGGAGTCAAATAAGGAAATGGTATGAATTTAATTTTGTTGATTTATTGTTTTCAGTACTAATTTATGCTAGACTAGATTGTTGATCATACTTACTGACTCATACACAGATGGTGAAACTGATGAACGTTTTGAAGCATCTCAATGCTCTTATCCAAACTGTTCACATATCAAGAAGTTGTTCTCTCATGCCAGTAAGTGCAAAATTCGAGTGAATGGGGGCTGCCAGCATTGTAAAAAGATATGGTTTATACTAACTACGCATTCCAGGAATTGCAAAGACTCGGAATGTAGAATACCGCGTTGCAGGTGTGTTACAATTAGAATTCTACATTGCCTAGAGAATTTTGATATTCTACTTTCTAACTTATTCTTTTGCTAAGCTATGCATAAATTTACTAATATATGAATACTTATTCTTTTATATTGATGTTGATTTTGCAGTGATCAGAAGAAGCATGTAGTAGAACGGAGATCAATGCATTCTGAATCTTGTCGTAGAGCTGATGTTTTGGAAAGCGACGGAGCAGTCATTGGTCAATGA
Protein sequence

>MS.gene068501.t1

MAANSDPTNNAVSQNRKIGELPKEVNPKIGYNTGKRWSNDENNVLKSVLDTKSWEDINWKDISNEEKLFCRDLKAIRDKARKLFKKERQDPKIRYNIESRGDYEKLLKQVSNRTVKELGQAKSSKDILVMQKVGQNILPTRENLIVAHLRSSCMHCREVTRKRWFYTECEKFQDCESCHGSDKHTSKNGEVHTLCQVAVDDFPSDTKHNDIVLESGLFENRDNFFIFCQKSQFQFDTLRRAKYSSMMNLHYLLRARNGETDERFEASQCSYPNCSHIKKLFSHASKCKIRVNGGCQHCKKIWFILTTHSRNCKDSECRIPRCSDQKKHVVERRSMHSESCRRADVLESDGAVIGQ