AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068626

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068626.t1 MTR_6g048540 66.667 117 39 0 568 684 106 222 5.63e-47 167
MS.gene068626.t1 MTR_6g048540 59.055 127 49 2 14 137 5 131 8.91e-42 152
MS.gene068626.t1 MTR_0734s0010 46.667 195 95 5 560 752 1 188 1.98e-44 158
MS.gene068626.t1 MTR_0853s0010 50.694 144 70 1 28 171 16 158 5.70e-42 150
MS.gene068626.t1 MTR_1g019850 54.930 142 56 1 32 173 65 198 7.20e-40 147
MS.gene068626.t1 MTR_6g051425 33.649 211 128 3 106 305 89 298 6.34e-35 136
MS.gene068626.t1 MTR_3g020080 52.336 107 30 2 385 487 3 92 4.05e-24 99.0
MS.gene068626.t1 MTR_0558s0020 25.940 266 171 5 34 275 33 296 4.76e-24 103
MS.gene068626.t1 MTR_8g446020 28.488 172 118 1 109 275 85 256 5.32e-21 93.6
MS.gene068626.t1 MTR_4g035820 27.444 266 168 7 30 279 68 324 7.17e-21 98.2
MS.gene068626.t1 MTR_4g035820 33.871 124 66 4 413 533 327 437 1.93e-11 67.8
MS.gene068626.t1 MTR_1g070355 24.068 295 177 9 9 269 28 309 1.55e-18 90.5
MS.gene068626.t1 MTR_6g022070 30.814 172 113 2 329 499 1 167 1.32e-17 85.5
MS.gene068626.t1 MTR_3g450850 26.136 264 163 9 39 281 49 301 1.84e-15 79.0
MS.gene068626.t1 MTR_6g090505 26.316 171 121 1 109 274 60 230 4.50e-15 78.2
MS.gene068626.t1 MTR_5g076070 26.820 261 166 9 18 263 32 282 1.76e-14 75.1
MS.gene068626.t1 MTR_8g466860 82.051 39 7 0 399 437 80 118 3.83e-14 70.1
MS.gene068626.t1 MTR_1g083640 22.363 237 158 5 34 246 48 282 4.72e-14 73.9
MS.gene068626.t1 MTR_7g446510 28.000 175 121 2 114 283 155 329 1.66e-13 72.8
MS.gene068626.t1 MTR_7g446480 28.977 176 118 4 114 283 140 314 2.56e-13 72.0
MS.gene068626.t1 MTR_7g446520 24.324 259 165 5 31 280 82 318 1.62e-12 69.7
MS.gene068626.t1 MTR_3g006370 34.426 122 64 5 31 144 44 157 7.89e-12 66.2
MS.gene068626.t1 MTR_2g024230 53.846 52 24 0 37 88 90 141 1.55e-11 63.5
MS.gene068626.t1 MTR_2g098880 28.916 166 96 5 5 163 42 192 2.56e-11 65.5
MS.gene068626.t1 MTR_2g098880 28.916 166 96 5 5 163 30 180 2.87e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068626.t1 AT4G38180 39.619 578 330 6 31 596 67 637 7.57e-157 475
MS.gene068626.t1 AT2G27110 35.188 665 376 15 1 646 1 629 3.24e-127 400
MS.gene068626.t1 AT2G27110 35.188 665 376 15 1 646 1 629 3.24e-127 400
MS.gene068626.t1 AT2G27110 41.458 439 252 3 208 646 51 484 1.06e-118 373
MS.gene068626.t1 AT4G15090 31.207 721 453 11 4 708 18 711 4.95e-114 364
MS.gene068626.t1 AT4G15090 31.207 721 453 11 4 708 18 711 4.95e-114 364
MS.gene068626.t1 AT4G15090 31.207 721 453 11 4 708 18 711 4.95e-114 364
MS.gene068626.t1 AT4G15090 31.207 721 453 11 4 708 18 711 4.95e-114 364
MS.gene068626.t1 AT4G15090 31.207 721 453 11 4 708 18 711 4.95e-114 364
MS.gene068626.t1 AT3G22170 33.111 601 362 9 11 592 38 617 4.98e-113 362
MS.gene068626.t1 AT3G22170 33.111 601 362 9 11 592 38 617 4.98e-113 362
MS.gene068626.t1 AT3G06250 33.011 621 353 12 35 646 188 754 4.28e-106 342
MS.gene068626.t1 AT3G06250 33.011 621 353 12 35 646 188 754 4.28e-106 342
MS.gene068626.t1 AT3G06250 33.011 621 353 12 35 646 188 754 4.28e-106 342
MS.gene068626.t1 AT1G76320 30.511 685 439 11 41 710 1 663 2.23e-103 332
MS.gene068626.t1 AT1G76320 30.511 685 439 11 41 710 1 663 1.59e-102 331
MS.gene068626.t1 AT1G76320 30.511 685 439 11 41 710 1 663 1.60e-102 332
MS.gene068626.t1 AT1G76320 30.511 685 439 11 41 710 1 663 1.60e-102 332
MS.gene068626.t1 AT5G18960 32.745 623 357 13 34 646 208 778 2.89e-102 332
MS.gene068626.t1 AT5G18960 32.745 623 357 13 34 646 208 778 2.89e-102 332
MS.gene068626.t1 AT4G38170 36.105 457 283 4 212 665 21 471 2.53e-98 315
MS.gene068626.t1 AT4G19990 32.767 589 335 10 34 601 18 566 9.20e-93 306
MS.gene068626.t1 AT4G19990 32.767 589 335 10 34 601 18 566 9.20e-93 306
MS.gene068626.t1 AT4G19990 32.767 589 335 10 34 601 18 566 1.73e-92 305
MS.gene068626.t1 AT4G19990 32.767 589 335 10 34 601 32 580 2.05e-92 305
MS.gene068626.t1 AT4G19990 33.219 584 339 11 34 601 18 566 2.28e-92 305
MS.gene068626.t1 AT4G19990 33.219 584 339 11 34 601 18 566 2.28e-92 305
MS.gene068626.t1 AT2G32250 29.421 656 404 12 10 659 12 614 6.52e-92 304
MS.gene068626.t1 AT2G32250 29.421 656 404 12 10 659 12 614 7.84e-92 305
MS.gene068626.t1 AT2G32250 29.421 656 404 12 10 659 12 614 7.84e-92 305
MS.gene068626.t1 AT2G32250 29.421 656 404 12 10 659 12 614 1.03e-91 305
MS.gene068626.t1 AT2G32250 29.421 656 404 12 10 659 12 614 1.03e-91 305
MS.gene068626.t1 AT2G32250 29.421 656 404 12 10 659 12 614 1.03e-91 305
MS.gene068626.t1 AT2G32250 29.469 621 388 10 41 659 1 573 2.03e-90 300
MS.gene068626.t1 AT2G32250 29.469 621 388 10 41 659 1 573 2.23e-90 300
MS.gene068626.t1 AT1G10240 31.130 575 363 11 34 590 45 604 4.63e-88 291
MS.gene068626.t1 AT1G10240 31.130 575 363 11 34 590 45 604 4.63e-88 291
MS.gene068626.t1 AT1G80010 31.179 619 365 16 7 594 74 662 3.23e-87 290
MS.gene068626.t1 AT1G52520 31.023 577 357 14 37 594 85 639 3.49e-87 290
MS.gene068626.t1 AT4G19990 33.797 503 288 6 106 601 45 509 2.42e-86 287
MS.gene068626.t1 AT5G28530 26.770 579 384 9 37 592 55 616 2.70e-69 241
MS.gene068626.t1 AT5G28530 26.770 579 384 9 37 592 55 616 2.70e-69 241
MS.gene068626.t1 AT5G28530 26.930 583 382 10 37 592 55 620 1.14e-68 239
MS.gene068626.t1 AT5G28530 24.737 570 344 11 37 592 55 553 1.29e-49 184
MS.gene068626.t1 AT5G28530 24.913 574 342 12 37 592 55 557 5.43e-49 183
MS.gene068626.t1 AT2G43280 31.159 138 79 5 19 148 4 133 1.06e-11 65.5
MS.gene068626.t1 AT3G07500 32.450 151 76 8 1 141 1 135 7.80e-11 63.2

Find 100 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCTCGTTGATGTAAATT+TGG 0.181383 5.1:+21319815 MS.gene068626:CDS
CTTCCTCCGACAAGCAATTT+TGG 0.188112 5.1:-21320229 None:intergenic
TTTACAATTCATTTGCAAAA+AGG 0.228560 5.1:+21318356 MS.gene068626:CDS
TGCATCCTTCTTAATATCTT+TGG 0.239826 5.1:-21320035 None:intergenic
GGGACTTGGCTTTCTCTAAT+AGG 0.243998 5.1:+21318749 MS.gene068626:CDS
AGATTAGCTTACCAACAATT+TGG 0.249704 5.1:+21318946 MS.gene068626:CDS
AGCAAAGGTATTAGTTGATT+TGG 0.254141 5.1:+21320070 MS.gene068626:CDS
GCCTCTGCTTATGGCATATT+AGG 0.270999 5.1:+21319229 MS.gene068626:CDS
GAGGTACATTAGAAGGAAAT+TGG 0.278387 5.1:+21318560 MS.gene068626:CDS
CAGAGGCGATGTCTAGTTTC+TGG 0.281760 5.1:-21319213 None:intergenic
GGAGTTTCTCCTTTGGAATC+TGG 0.287253 5.1:+21320194 MS.gene068626:CDS
CTAGACATCGCCTCTGCTTA+TGG 0.301818 5.1:+21319220 MS.gene068626:CDS
ATGCCATTTGCCCCGTTTGT+TGG 0.307675 5.1:+21319015 MS.gene068626:CDS
GGATTTCTAATAGTCAATTC+TGG 0.312090 5.1:-21320260 None:intergenic
CTGGTCAGTTATGACAGATT+TGG 0.312946 5.1:-21319154 None:intergenic
GGTAAATTTCAAGATGAGAT+TGG 0.337382 5.1:+21319702 MS.gene068626:CDS
ATTAAATAGTGAACCTTGTA+AGG 0.338372 5.1:+21318297 MS.gene068626:CDS
CATACAAAGACAGCTCTCTT+TGG 0.340587 5.1:-21318412 None:intergenic
TGGTCAGTTATGACAGATTT+GGG 0.341273 5.1:-21319153 None:intergenic
GGACTTGGCTTTCTCTAATA+GGG 0.341505 5.1:+21318750 MS.gene068626:CDS
TATATAAAATTAGAGAATCT+TGG 0.344745 5.1:+21319418 MS.gene068626:CDS
AGTCAGAACGCTCATTTATA+TGG 0.347581 5.1:+21319091 MS.gene068626:CDS
ATCTCGAGCATTGTAGTTGC+TGG 0.348302 5.1:-21319790 None:intergenic
TGTCATAACTGACCAGGATT+TGG 0.348459 5.1:+21319161 MS.gene068626:CDS
TTTAATTCGTCACCAACATT+AGG 0.350028 5.1:-21318280 None:intergenic
CAGTGATGGAGTTTCTCCTT+TGG 0.356600 5.1:+21320187 MS.gene068626:CDS
TCCAACGCTGCAAAATAAAA+TGG 0.360936 5.1:-21319944 None:intergenic
TCTTCAAAACTTTCTATACT+TGG 0.373106 5.1:-21319336 None:intergenic
TAGTTTGATTTGTGTCATCT+TGG 0.378031 5.1:-21318483 None:intergenic
TTGGCTATGAAAGCTGCTTT+AGG 0.380570 5.1:+21319180 MS.gene068626:CDS
CTTTCTCTAATAGGGATTGT+TGG 0.385066 5.1:+21318758 MS.gene068626:CDS
AAACAGCTTCTATTATAAAC+TGG 0.392497 5.1:-21319444 None:intergenic
CAATTATTGCAAACAGAAAC+AGG 0.397066 5.1:+21318840 MS.gene068626:CDS
GAAACTTGATAGACATAGTT+AGG 0.397106 5.1:-21319768 None:intergenic
GATATTGTGCAGGCATATAC+TGG 0.399955 5.1:+21319884 MS.gene068626:CDS
ATATTACATCTCCAAATTGT+TGG 0.402900 5.1:-21318957 None:intergenic
GATTATTCAATCCAACAAAC+GGG 0.413405 5.1:-21319026 None:intergenic
AATGTTCAGTGAAGGGGACT+TGG 0.418030 5.1:+21318735 MS.gene068626:CDS
GTAAATTTCAAGATGAGATT+GGG 0.418089 5.1:+21319703 MS.gene068626:CDS
GTCAAAAGAGGTACATTAGA+AGG 0.419461 5.1:+21318553 MS.gene068626:CDS
GTCCGTACTCAATCATTAAT+CGG 0.426232 5.1:-21319368 None:intergenic
AAGTGTTCTACTTCTCGAAC+TGG 0.426697 5.1:+21318511 MS.gene068626:CDS
TCCAATGTGATGAAGATTCT+CGG 0.434696 5.1:+21318890 MS.gene068626:CDS
CCACGCAATGCACAAACAAA+AGG 0.435072 5.1:+21320281 MS.gene068626:CDS
GACAACAATGAATGGCTTCA+AGG 0.439948 5.1:+21319393 MS.gene068626:CDS
AATTCATTATATCTGATTTG+AGG 0.445916 5.1:+21320117 MS.gene068626:CDS
CGCTGCAAAATAAAATGGTC+AGG 0.446582 5.1:-21319939 None:intergenic
GGAATTCAAATCTGTGGAAA+AGG 0.451124 5.1:+21318324 MS.gene068626:CDS
TGTCATGAGTGTGGTGAATC+TGG 0.451337 5.1:+21320401 MS.gene068626:CDS
ATTTGTGTTGAAATTTGAAA+AGG 0.454017 5.1:+21319554 MS.gene068626:CDS
AATCCAACAAACGGGGCAAA+TGG 0.460756 5.1:-21319018 None:intergenic
TGATTATTCAATCCAACAAA+CGG 0.469113 5.1:-21319027 None:intergenic
TTTCAATTCTCGCTTGAAAG+TGG 0.477143 5.1:-21319295 None:intergenic
TTGAATTCCATCCCCTTACA+AGG 0.484462 5.1:-21318310 None:intergenic
TTAGCTGCTGCTCCTAATGT+TGG 0.490364 5.1:+21318268 MS.gene068626:CDS
AAAGAGAGGATTATGAAAGT+AGG 0.500092 5.1:+21319604 MS.gene068626:CDS
ATTTGATTTCTTCATTGCAA+TGG 0.501662 5.1:-21320156 None:intergenic
GCCACACTTGTTGTCAAAAG+AGG 0.505706 5.1:+21318541 MS.gene068626:CDS
CAAAATTGTAGATTCAAAAG+TGG 0.511466 5.1:+21320332 MS.gene068626:CDS
GTCTTTGTATGTTGTAATGA+AGG 0.517879 5.1:+21318424 MS.gene068626:CDS
TGATTTGAGGCGCACTCGTA+TGG 0.520232 5.1:+21320130 MS.gene068626:CDS
ATACTTGTTAGTCTTATATG+TGG 0.524306 5.1:-21318989 None:intergenic
TCCGAGAATCTTCATCACAT+TGG 0.530876 5.1:-21318891 None:intergenic
TAGTGAACCTTGTAAGGGGA+TGG 0.531310 5.1:+21318303 MS.gene068626:CDS
CACGCAATGCACAAACAAAA+GGG 0.538817 5.1:+21320282 MS.gene068626:CDS
AAGGAAGAAGGTATACCGAC+AGG 0.538923 5.1:+21318700 MS.gene068626:CDS
TGTCAACCAAAATTGCTTGT+CGG 0.544243 5.1:+21320223 MS.gene068626:CDS
CAGCTTTCATAGCCAAATCC+TGG 0.547648 5.1:-21319173 None:intergenic
TCCTAATATGCCATAAGCAG+AGG 0.549443 5.1:-21319230 None:intergenic
CGGTGTCATAAAAGCATGAA+TGG 0.550442 5.1:+21318649 MS.gene068626:CDS
CAAATCTGTCATAACTGACC+AGG 0.551634 5.1:+21319155 MS.gene068626:CDS
TCAAATTTAGCTATCAGTGA+TGG 0.553213 5.1:+21320173 MS.gene068626:CDS
CGAAGAAAGAATTTAGACGT+TGG 0.559738 5.1:+21318799 MS.gene068626:CDS
TTAAATAGTGAACCTTGTAA+GGG 0.561628 5.1:+21318298 MS.gene068626:CDS
GCACGCCAAAGATATTAAGA+AGG 0.572798 5.1:+21320030 MS.gene068626:CDS
ACAACAATGAATGGCTTCAA+GGG 0.573168 5.1:+21319394 MS.gene068626:CDS
ACGGACTGGACAACAATGAA+TGG 0.575271 5.1:+21319385 MS.gene068626:CDS
TGCTACAATGTTCAGTGAAG+GGG 0.575975 5.1:+21318729 MS.gene068626:CDS
TTGCTACAATGTTCAGTGAA+GGG 0.579493 5.1:+21318728 MS.gene068626:CDS
GTCTACAGGGTCAAAACTTG+AGG 0.581238 5.1:+21319644 MS.gene068626:CDS
GGGGATGGAATTCAAATCTG+TGG 0.582477 5.1:+21318318 MS.gene068626:CDS
TGTGTTGAAATTTGAAAAGG+CGG 0.586171 5.1:+21319557 MS.gene068626:CDS
AGCCGATTAATGATTGAGTA+CGG 0.595364 5.1:+21319366 MS.gene068626:CDS
ATTGTAGCAACCTTTCCTGT+CGG 0.600908 5.1:-21318715 None:intergenic
ACAAACAAAAGGGAGAAAGA+AGG 0.601052 5.1:+21320292 MS.gene068626:CDS
GTTGCTACAATGTTCAGTGA+AGG 0.603702 5.1:+21318727 MS.gene068626:CDS
GCATGCTCAACAAGAAGCAA+AGG 0.605686 5.1:+21320055 MS.gene068626:CDS
TTCCACATTTGTTGACTGTG+TGG 0.606600 5.1:-21318227 None:intergenic
ATTAATGATTGAGTACGGAC+TGG 0.607432 5.1:+21319371 MS.gene068626:CDS
GTGCATTGGTCAATTATGGA+TGG 0.620199 5.1:+21318187 None:intergenic
AGAAGGTATACCGACAGGAA+AGG 0.621900 5.1:+21318705 MS.gene068626:CDS
AGCCACACAGTCAACAAATG+TGG 0.622019 5.1:+21318225 MS.gene068626:CDS
ACAAAAGGGAGAAAGAAGGA+TGG 0.625355 5.1:+21320296 MS.gene068626:CDS
TAAATAGTGAACCTTGTAAG+GGG 0.625905 5.1:+21318299 MS.gene068626:CDS
GACACACAGATTGCAAAATG+TGG 0.629854 5.1:+21319837 MS.gene068626:CDS
CAACCAAAATTGCTTGTCGG+AGG 0.633822 5.1:+21320226 MS.gene068626:CDS
GAATGATCATAACCATCCCA+TGG 0.640152 5.1:+21318600 MS.gene068626:CDS
CTAAAAGTGTGTCTTATATG+CGG 0.670716 5.1:+21318629 MS.gene068626:CDS
AGAAAAGCATGTCATGAGTG+TGG 0.709074 5.1:+21320392 MS.gene068626:CDS
ATTATTCAATCCAACAAACG+GGG 0.719290 5.1:-21319025 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATAAAATTAGAGAATCT+TGG + chr5.1:21319418-21319437 MS.gene068626:CDS 15.0%
!!! CATTATCAATCAATTTTATT+TGG + chr5.1:21319048-21319067 MS.gene068626:CDS 15.0%
!! ATTTGTGTTGAAATTTGAAA+AGG + chr5.1:21319554-21319573 MS.gene068626:CDS 20.0%
!! TTTACAATTCATTTGCAAAA+AGG + chr5.1:21318356-21318375 MS.gene068626:CDS 20.0%
!!! AATTCATTATATCTGATTTG+AGG + chr5.1:21320117-21320136 MS.gene068626:CDS 20.0%
!!! ATGTCAATTATATGAGTTTT+TGG + chr5.1:21319860-21319879 MS.gene068626:CDS 20.0%
!!! ATTTATTCGAGAAACATTTT+TGG + chr5.1:21319681-21319700 MS.gene068626:CDS 20.0%
!!! TGTCAATTATATGAGTTTTT+GGG + chr5.1:21319861-21319880 MS.gene068626:CDS 20.0%
! AAAAAGAAGATTAGCAAAGA+TGG + chr5.1:21319744-21319763 MS.gene068626:CDS 25.0%
! AAACAGCTTCTATTATAAAC+TGG - chr5.1:21319447-21319466 None:intergenic 25.0%
! AATTCATTTGCAAAAAGGAT+GGG + chr5.1:21318361-21318380 MS.gene068626:CDS 25.0%
! ATATTACATCTCCAAATTGT+TGG - chr5.1:21318960-21318979 None:intergenic 25.0%
! ATTAAATAGTGAACCTTGTA+AGG + chr5.1:21318297-21318316 MS.gene068626:CDS 25.0%
! CAAAATTGTAGATTCAAAAG+TGG + chr5.1:21320332-21320351 MS.gene068626:CDS 25.0%
! GTAAATTTCAAGATGAGATT+GGG + chr5.1:21319703-21319722 MS.gene068626:CDS 25.0%
! TCTTCAAAACTTTCTATACT+TGG - chr5.1:21319339-21319358 None:intergenic 25.0%
! TGATTATTCAATCCAACAAA+CGG - chr5.1:21319030-21319049 None:intergenic 25.0%
! TTAAATAGTGAACCTTGTAA+GGG + chr5.1:21318298-21318317 MS.gene068626:CDS 25.0%
!! ATTTGATTTCTTCATTGCAA+TGG - chr5.1:21320159-21320178 None:intergenic 25.0%
!! GTCAATTATATGAGTTTTTG+GGG + chr5.1:21319862-21319881 MS.gene068626:CDS 25.0%
!!! AAGTCTTGTTGAACATTTTA+AGG + chr5.1:21318681-21318700 MS.gene068626:CDS 25.0%
!!! ATACTTGTTAGTCTTATATG+TGG - chr5.1:21318992-21319011 None:intergenic 25.0%
!!! GTTGTTTGAAACTTTTCTTA+AGG + chr5.1:21319113-21319132 MS.gene068626:CDS 25.0%
!!! TCGGATGATAAACTTTTTTT+GGG + chr5.1:21318909-21318928 MS.gene068626:CDS 25.0%
AAAAAACGCATATTGTCTAC+AGG + chr5.1:21319630-21319649 MS.gene068626:CDS 30.0%
AAAAACGCATATTGTCTACA+GGG + chr5.1:21319631-21319650 MS.gene068626:CDS 30.0%
AAAGAGAGGATTATGAAAGT+AGG + chr5.1:21319604-21319623 MS.gene068626:CDS 30.0%
ATTATTCAATCCAACAAACG+GGG - chr5.1:21319028-21319047 None:intergenic 30.0%
CAATTATTGCAAACAGAAAC+AGG + chr5.1:21318840-21318859 MS.gene068626:CDS 30.0%
CAATTCATTTGCAAAAAGGA+TGG + chr5.1:21318360-21318379 MS.gene068626:CDS 30.0%
CTTAGAAGTAGAAAAAAGAG+AGG + chr5.1:21319590-21319609 MS.gene068626:CDS 30.0%
GAAACTTGATAGACATAGTT+AGG - chr5.1:21319771-21319790 None:intergenic 30.0%
GATTATTCAATCCAACAAAC+GGG - chr5.1:21319029-21319048 None:intergenic 30.0%
GGATTTCTAATAGTCAATTC+TGG - chr5.1:21320263-21320282 None:intergenic 30.0%
GGTAAATTTCAAGATGAGAT+TGG + chr5.1:21319702-21319721 MS.gene068626:CDS 30.0%
GTCTTTGTATGTTGTAATGA+AGG + chr5.1:21318424-21318443 MS.gene068626:CDS 30.0%
GTTTCTCGTTGATGTAAATT+TGG + chr5.1:21319815-21319834 MS.gene068626:CDS 30.0%
TAAATAGTGAACCTTGTAAG+GGG + chr5.1:21318299-21318318 MS.gene068626:CDS 30.0%
TCAAATTTAGCTATCAGTGA+TGG + chr5.1:21320173-21320192 MS.gene068626:CDS 30.0%
TGCATCCTTCTTAATATCTT+TGG - chr5.1:21320038-21320057 None:intergenic 30.0%
TGTGTTGAAATTTGAAAAGG+CGG + chr5.1:21319557-21319576 MS.gene068626:CDS 30.0%
TTTAATTCGTCACCAACATT+AGG - chr5.1:21318283-21318302 None:intergenic 30.0%
! CTAAAAGTGTGTCTTATATG+CGG + chr5.1:21318629-21318648 MS.gene068626:CDS 30.0%
! GCATATAAGACACACTTTTA+GGG - chr5.1:21318630-21318649 None:intergenic 30.0%
! TGAAACTTTTCTTAAGGCAA+TGG + chr5.1:21319119-21319138 MS.gene068626:CDS 30.0%
!! AGATTAGCTTACCAACAATT+TGG + chr5.1:21318946-21318965 MS.gene068626:CDS 30.0%
!! AGCAAAGGTATTAGTTGATT+TGG + chr5.1:21320070-21320089 MS.gene068626:CDS 30.0%
!! GTTGAACATTTTAAGGAAGA+AGG + chr5.1:21318688-21318707 MS.gene068626:CDS 30.0%
!! TAGTTTGATTTGTGTCATCT+TGG - chr5.1:21318486-21318505 None:intergenic 30.0%
!!! AATAGAAGCTGTTTTTTTGC+TGG + chr5.1:21319453-21319472 MS.gene068626:CDS 30.0%
!!! AGCAATTTTGGTTGACATTT+TGG - chr5.1:21320220-21320239 None:intergenic 30.0%
!!! ATAGAAGCTGTTTTTTTGCT+GGG + chr5.1:21319454-21319473 MS.gene068626:CDS 30.0%
!!! CTCGGATGATAAACTTTTTT+TGG + chr5.1:21318908-21318927 MS.gene068626:CDS 30.0%
!!! CTGGTAATTTTTCAAGCAAA+AGG + chr5.1:21319903-21319922 MS.gene068626:CDS 30.0%
!!! TAGAAAGTTTTGAAGAAGAC+TGG + chr5.1:21319343-21319362 MS.gene068626:CDS 30.0%
ACAAACAAAAGGGAGAAAGA+AGG + chr5.1:21320292-21320311 MS.gene068626:CDS 35.0%
ACAACAATGAATGGCTTCAA+GGG + chr5.1:21319394-21319413 MS.gene068626:CDS 35.0%
AGCCGATTAATGATTGAGTA+CGG + chr5.1:21319366-21319385 MS.gene068626:CDS 35.0%
AGTCAGAACGCTCATTTATA+TGG + chr5.1:21319091-21319110 MS.gene068626:CDS 35.0%
ATTAATGATTGAGTACGGAC+TGG + chr5.1:21319371-21319390 MS.gene068626:CDS 35.0%
CGAAGAAAGAATTTAGACGT+TGG + chr5.1:21318799-21318818 MS.gene068626:CDS 35.0%
CTTTCTCTAATAGGGATTGT+TGG + chr5.1:21318758-21318777 MS.gene068626:CDS 35.0%
GAGGTACATTAGAAGGAAAT+TGG + chr5.1:21318560-21318579 MS.gene068626:CDS 35.0%
GGAATTCAAATCTGTGGAAA+AGG + chr5.1:21318324-21318343 MS.gene068626:CDS 35.0%
GTCCGTACTCAATCATTAAT+CGG - chr5.1:21319371-21319390 None:intergenic 35.0%
TCCAACGCTGCAAAATAAAA+TGG - chr5.1:21319947-21319966 None:intergenic 35.0%
TCCAATGTGATGAAGATTCT+CGG + chr5.1:21318890-21318909 MS.gene068626:CDS 35.0%
TGGTCAGTTATGACAGATTT+GGG - chr5.1:21319156-21319175 None:intergenic 35.0%
TGTCAACCAAAATTGCTTGT+CGG + chr5.1:21320223-21320242 MS.gene068626:CDS 35.0%
TTGCTACAATGTTCAGTGAA+GGG + chr5.1:21318728-21318747 MS.gene068626:CDS 35.0%
TTTCAATTCTCGCTTGAAAG+TGG - chr5.1:21319298-21319317 None:intergenic 35.0%
! ACTTTTCTTAAGGCAATGGA+TGG + chr5.1:21319123-21319142 MS.gene068626:CDS 35.0%
! CGCATATAAGACACACTTTT+AGG - chr5.1:21318631-21318650 None:intergenic 35.0%
! GATACGACGAGTAATTTTGA+TGG + chr5.1:21319999-21320018 MS.gene068626:CDS 35.0%
! GTCAAAAGAGGTACATTAGA+AGG + chr5.1:21318553-21318572 MS.gene068626:CDS 35.0%
!! TTTGCAAAAAGGATGGGTTT+TGG + chr5.1:21318367-21318386 MS.gene068626:CDS 35.0%
!!! ACCATTTTATTTTGCAGCGT+TGG + chr5.1:21319943-21319962 MS.gene068626:CDS 35.0%
AAGTGTTCTACTTCTCGAAC+TGG + chr5.1:21318511-21318530 MS.gene068626:CDS 40.0%
ACAAAAGGGAGAAAGAAGGA+TGG + chr5.1:21320296-21320315 MS.gene068626:CDS 40.0%
ATTGTAGCAACCTTTCCTGT+CGG - chr5.1:21318718-21318737 None:intergenic 40.0%
CAAATCTGTCATAACTGACC+AGG + chr5.1:21319155-21319174 MS.gene068626:CDS 40.0%
CACGCAATGCACAAACAAAA+GGG + chr5.1:21320282-21320301 MS.gene068626:CDS 40.0%
CATACAAAGACAGCTCTCTT+TGG - chr5.1:21318415-21318434 None:intergenic 40.0%
CGCTGCAAAATAAAATGGTC+AGG - chr5.1:21319942-21319961 None:intergenic 40.0%
CTGGTCAGTTATGACAGATT+TGG - chr5.1:21319157-21319176 None:intergenic 40.0%
GAATGATCATAACCATCCCA+TGG + chr5.1:21318600-21318619 MS.gene068626:CDS 40.0%
GACAACAATGAATGGCTTCA+AGG + chr5.1:21319393-21319412 MS.gene068626:CDS 40.0%
GACACACAGATTGCAAAATG+TGG + chr5.1:21319837-21319856 MS.gene068626:CDS 40.0%
GATATTGTGCAGGCATATAC+TGG + chr5.1:21319884-21319903 MS.gene068626:CDS 40.0%
GCACGCCAAAGATATTAAGA+AGG + chr5.1:21320030-21320049 MS.gene068626:CDS 40.0%
GTTGCTACAATGTTCAGTGA+AGG + chr5.1:21318727-21318746 MS.gene068626:CDS 40.0%
TCCGAGAATCTTCATCACAT+TGG - chr5.1:21318894-21318913 None:intergenic 40.0%
TCCTAATATGCCATAAGCAG+AGG - chr5.1:21319233-21319252 None:intergenic 40.0%
TGCTACAATGTTCAGTGAAG+GGG + chr5.1:21318729-21318748 MS.gene068626:CDS 40.0%
TGTCATAACTGACCAGGATT+TGG + chr5.1:21319161-21319180 MS.gene068626:CDS 40.0%
TTGAATTCCATCCCCTTACA+AGG - chr5.1:21318313-21318332 None:intergenic 40.0%
! ACCTCTTTTGACAACAAGTG+TGG - chr5.1:21318545-21318564 None:intergenic 40.0%
! AGAAAAGCATGTCATGAGTG+TGG + chr5.1:21320392-21320411 MS.gene068626:CDS 40.0%
! GGACTTGGCTTTCTCTAATA+GGG + chr5.1:21318750-21318769 MS.gene068626:CDS 40.0%
! TTCCACATTTGTTGACTGTG+TGG - chr5.1:21318230-21318249 None:intergenic 40.0%
! TTGGCTATGAAAGCTGCTTT+AGG + chr5.1:21319180-21319199 MS.gene068626:CDS 40.0%
!! ACATTTTGGCCAGATTCCAA+AGG - chr5.1:21320206-21320225 None:intergenic 40.0%
!! AGTTTTTGGGGATATTGTGC+AGG + chr5.1:21319874-21319893 MS.gene068626:CDS 40.0%
!! CGGTGTCATAAAAGCATGAA+TGG + chr5.1:21318649-21318668 MS.gene068626:CDS 40.0%
!!! ACACTTTTAGGGCTAACCAT+GGG - chr5.1:21318619-21318638 None:intergenic 40.0%
AATCCAACAAACGGGGCAAA+TGG - chr5.1:21319021-21319040 None:intergenic 45.0%
AATGTTCAGTGAAGGGGACT+TGG + chr5.1:21318735-21318754 MS.gene068626:CDS 45.0%
AGCCACACAGTCAACAAATG+TGG + chr5.1:21318225-21318244 MS.gene068626:CDS 45.0%
CAACCAAAATTGCTTGTCGG+AGG + chr5.1:21320226-21320245 MS.gene068626:CDS 45.0%
CAGCTTTCATAGCCAAATCC+TGG - chr5.1:21319176-21319195 None:intergenic 45.0%
CCACGCAATGCACAAACAAA+AGG + chr5.1:21320281-21320300 MS.gene068626:CDS 45.0%
GCATGCTCAACAAGAAGCAA+AGG + chr5.1:21320055-21320074 MS.gene068626:CDS 45.0%
GCCTCTGCTTATGGCATATT+AGG + chr5.1:21319229-21319248 MS.gene068626:CDS 45.0%
GGGGATGGAATTCAAATCTG+TGG + chr5.1:21318318-21318337 MS.gene068626:CDS 45.0%
GTCTACAGGGTCAAAACTTG+AGG + chr5.1:21319644-21319663 MS.gene068626:CDS 45.0%
TAGTGAACCTTGTAAGGGGA+TGG + chr5.1:21318303-21318322 MS.gene068626:CDS 45.0%
TGTCATGAGTGTGGTGAATC+TGG + chr5.1:21320401-21320420 MS.gene068626:CDS 45.0%
TTAGCTGCTGCTCCTAATGT+TGG + chr5.1:21318268-21318287 MS.gene068626:CDS 45.0%
! AAGGAAGAAGGTATACCGAC+AGG + chr5.1:21318700-21318719 MS.gene068626:CDS 45.0%
! AGAAGGTATACCGACAGGAA+AGG + chr5.1:21318705-21318724 MS.gene068626:CDS 45.0%
! ATCTCGAGCATTGTAGTTGC+TGG - chr5.1:21319793-21319812 None:intergenic 45.0%
! CAGTGATGGAGTTTCTCCTT+TGG + chr5.1:21320187-21320206 MS.gene068626:CDS 45.0%
! CTTCCTCCGACAAGCAATTT+TGG - chr5.1:21320232-21320251 None:intergenic 45.0%
! GGGACTTGGCTTTCTCTAAT+AGG + chr5.1:21318749-21318768 MS.gene068626:CDS 45.0%
!! ACGGACTGGACAACAATGAA+TGG + chr5.1:21319385-21319404 MS.gene068626:CDS 45.0%
!! CCTTTTGTTTGTGCATTGCG+TGG - chr5.1:21320284-21320303 None:intergenic 45.0%
!! GCCACACTTGTTGTCAAAAG+AGG + chr5.1:21318541-21318560 MS.gene068626:CDS 45.0%
!! GGAGTTTCTCCTTTGGAATC+TGG + chr5.1:21320194-21320213 MS.gene068626:CDS 45.0%
!!! CACACTTTTAGGGCTAACCA+TGG - chr5.1:21318620-21318639 None:intergenic 45.0%
!!! TTTTAGGGCTAACCATGGGA+TGG - chr5.1:21318615-21318634 None:intergenic 45.0%
CTAGACATCGCCTCTGCTTA+TGG + chr5.1:21319220-21319239 MS.gene068626:CDS 50.0%
! ATGCCATTTGCCCCGTTTGT+TGG + chr5.1:21319015-21319034 MS.gene068626:CDS 50.0%
! CAGAGGCGATGTCTAGTTTC+TGG - chr5.1:21319216-21319235 None:intergenic 50.0%
! TGATTTGAGGCGCACTCGTA+TGG + chr5.1:21320130-21320149 MS.gene068626:CDS 50.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 21318202 21320466 21318202 ID=MS.gene068626
chr5.1 mRNA 21318202 21320466 21318202 ID=MS.gene068626.t1;Parent=MS.gene068626
chr5.1 exon 21318202 21320466 21318202 ID=MS.gene068626.t1.exon1;Parent=MS.gene068626.t1
chr5.1 CDS 21318202 21320466 21318202 ID=cds.MS.gene068626.t1;Parent=MS.gene068626.t1
Gene Sequence

>MS.gene068626

ATGGATGGTAATGTTTTTGAAGAAGCCACACAGTCAACAAATGTGGAAATATCTGAAGAAGATATTTTAGCTGCTGCTCCTAATGTTGGTGACGAATTAAATAGTGAACCTTGTAAGGGGATGGAATTCAAATCTGTGGAAAAGGTTAGAGAATTTTACAATTCATTTGCAAAAAGGATGGGTTTTGGAGTACGTGTTCGCTCAACTAAACCAAAGAGAGCTGTCTTTGTATGTTGTAATGAAGGTCATCATATAGTGAAAAGTTCGATAAACAAAGAAATCCAAGATGACACAAATCAAACTAAAAGAAAGTGTTCTACTTCTCGAACTGGTTGTCTAGCCACACTTGTTGTCAAAAGAGGTACATTAGAAGGAAATTGGATTATATGTTCATTTAAGAATGATCATAACCATCCCATGGTTAGCCCTAAAAGTGTGTCTTATATGCGGTGTCATAAAAGCATGAATGGTGCTGCAAAAAGTCTTGTTGAACATTTTAAGGAAGAAGGTATACCGACAGGAAAGGTTGCTACAATGTTCAGTGAAGGGGACTTGGCTTTCTCTAATAGGGATTGTTGGAATCATGTCAGAAGTCTTCGAAGAAAGAATTTAGACGTTGGAGATGCTCAAGCTGTTTTCAATTATTGCAAACAGAAACAGGTAGAAAATTCAAACTTTTTCTATGAAATCCAATGTGATGAAGATTCTCGGATGATAAACTTTTTTTGGGTTGATGCTAGATCAAGATTAGCTTACCAACAATTTGGAGATGTAATATCTTTTGACACCACATATAAGACTAACAAGTATAGTATGCCATTTGCCCCGTTTGTTGGATTGAATAATCATTATCAATCAATTTTATTTGGTTGTGCTTTACTACAAGACGAGTCAGAACGCTCATTTATATGGTTGTTTGAAACTTTTCTTAAGGCAATGGATGGTAAAAAGCCCAAATCTGTCATAACTGACCAGGATTTGGCTATGAAAGCTGCTTTAGGCAAAGTATTTCCAGAAACTAGACATCGCCTCTGCTTATGGCATATTAGGAAAAAATTTGCTGAAAAGTTAGCTCATGTTTATCATAAAAATTCCACTTTCAAGCGAGAATTGAAAAAATGCATTCATGTTTCACCAAGTATAGAAAGTTTTGAAGAAGACTGGAGCCGATTAATGATTGAGTACGGACTGGACAACAATGAATGGCTTCAAGGGTTATATAAAATTAGAGAATCTTGGATACCAGTTTATAATAGAAGCTGTTTTTTTGCTGGGATGAATACAAGTCAAAGAAGTGAGAGCATCAATGCGTTTTTTGATTCTTTTGTGAATGCAACTACAACATTGCAAGAATTTGTGTTGAAATTTGAAAAGGCGGTTGATAGCTGCTTAGAAGTAGAAAAAAGAGAGGATTATGAAAGTAGGCATAAAAAACGCATATTGTCTACAGGGTCAAAACTTGAGGAGCACGCTGCATCTATTTATTCGAGAAACATTTTTGGTAAATTTCAAGATGAGATTGGGAAGATCTACAAATTTGCAAAAAAGAAGATTAGCAAAGATGGACCTAACTATGTCTATCAAGTTTCCAGCAACTACAATGCTCGAGATATGTTTCTCGTTGATGTAAATTTGGACACACAGATTGCAAAATGTGGATGTCAATTATATGAGTTTTTGGGGATATTGTGCAGGCATATACTGGTAATTTTTCAAGCAAAAGGTATCACTCAAATTCCTGACCATTTTATTTTGCAGCGTTGGACTAAAAATGCTAACAAAAGCATTGAAGTTTGTGATACGACGAGTAATTTTGATGGTCAAAATAGCACGCCAAAGATATTAAGAAGGATGCATGCTCAACAAGAAGCAAAGGTATTAGTTGATTTGGCTGAAGAGTCTGATGAGATATACAAATTCATTATATCTGATTTGAGGCGCACTCGTATGGCAGCCATTGCAATGAAGAAATCAAATTTAGCTATCAGTGATGGAGTTTCTCCTTTGGAATCTGGCCAAAATGTCAACCAAAATTGCTTGTCGGAGGAAGAAAGTGAACCAGAATTGACTATTAGAAATCCACGCAATGCACAAACAAAAGGGAGAAAGAAGGATGGACAGAAAAACACTCAAAATTGTAGATTCAAAAGTGGAATTGAACTGTCACATGCTCAACAAGTAGCAGTCAAAAGAAAAGCATGTCATGAGTGTGGTGAATCTGGTCACAACAGCAGAACTTGCAAGAAAAGAAATCAAAATGATTAA
Protein sequence

>MS.gene068626.t1

MDGNVFEEATQSTNVEISEEDILAAAPNVGDELNSEPCKGMEFKSVEKVREFYNSFAKRMGFGVRVRSTKPKRAVFVCCNEGHHIVKSSINKEIQDDTNQTKRKCSTSRTGCLATLVVKRGTLEGNWIICSFKNDHNHPMVSPKSVSYMRCHKSMNGAAKSLVEHFKEEGIPTGKVATMFSEGDLAFSNRDCWNHVRSLRRKNLDVGDAQAVFNYCKQKQVENSNFFYEIQCDEDSRMINFFWVDARSRLAYQQFGDVISFDTTYKTNKYSMPFAPFVGLNNHYQSILFGCALLQDESERSFIWLFETFLKAMDGKKPKSVITDQDLAMKAALGKVFPETRHRLCLWHIRKKFAEKLAHVYHKNSTFKRELKKCIHVSPSIESFEEDWSRLMIEYGLDNNEWLQGLYKIRESWIPVYNRSCFFAGMNTSQRSESINAFFDSFVNATTTLQEFVLKFEKAVDSCLEVEKREDYESRHKKRILSTGSKLEEHAASIYSRNIFGKFQDEIGKIYKFAKKKISKDGPNYVYQVSSNYNARDMFLVDVNLDTQIAKCGCQLYEFLGILCRHILVIFQAKGITQIPDHFILQRWTKNANKSIEVCDTTSNFDGQNSTPKILRRMHAQQEAKVLVDLAEESDEIYKFIISDLRRTRMAAIAMKKSNLAISDGVSPLESGQNVNQNCLSEEESEPELTIRNPRNAQTKGRKKDGQKNTQNCRFKSGIELSHAQQVAVKRKACHECGESGHNSRTCKKRNQND