Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068870.t1 | XP_013468829.1 | 95 | 159 | 8 | 0 | 4 | 162 | 1 | 159 | 1.30E-75 | 292.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068870.t1 | Q9FJK3 | 46.2 | 156 | 83 | 1 | 4 | 158 | 1 | 156 | 1.0e-28 | 127.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068870.t1 | A0A072VMQ1 | 95.0 | 159 | 8 | 0 | 4 | 162 | 1 | 159 | 9.6e-76 | 292.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene068870.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068870.t1 | MTR_1g077320 | 94.969 | 159 | 8 | 0 | 4 | 162 | 1 | 159 | 2.05e-101 | 288 |
| MS.gene068870.t1 | MTR_7g011950 | 89.937 | 159 | 16 | 0 | 4 | 162 | 1 | 159 | 8.19e-96 | 274 |
| MS.gene068870.t1 | MTR_4g028720 | 86.792 | 159 | 21 | 0 | 4 | 162 | 1 | 159 | 1.55e-93 | 268 |
| MS.gene068870.t1 | MTR_1g077390 | 70.701 | 157 | 46 | 0 | 4 | 160 | 1 | 157 | 1.52e-78 | 230 |
| MS.gene068870.t1 | MTR_5g047580 | 70.513 | 156 | 46 | 0 | 4 | 159 | 1 | 156 | 1.50e-76 | 225 |
| MS.gene068870.t1 | MTR_1g084950 | 74.359 | 156 | 40 | 0 | 4 | 159 | 1 | 156 | 3.37e-75 | 222 |
| MS.gene068870.t1 | MTR_1g077300 | 74.843 | 159 | 38 | 1 | 4 | 162 | 1 | 157 | 1.25e-74 | 220 |
| MS.gene068870.t1 | MTR_1g090697 | 56.129 | 155 | 68 | 0 | 4 | 158 | 1 | 155 | 4.41e-59 | 181 |
| MS.gene068870.t1 | MTR_5g047560 | 58.974 | 156 | 48 | 1 | 4 | 159 | 1 | 140 | 3.99e-58 | 178 |
| MS.gene068870.t1 | MTR_1g090710 | 55.128 | 156 | 69 | 1 | 4 | 158 | 1 | 156 | 1.78e-57 | 177 |
| MS.gene068870.t1 | MTR_1g090783 | 54.902 | 153 | 69 | 0 | 6 | 158 | 2 | 154 | 1.11e-55 | 172 |
| MS.gene068870.t1 | MTR_3g065100 | 51.592 | 157 | 75 | 1 | 4 | 159 | 1 | 157 | 2.84e-41 | 138 |
| MS.gene068870.t1 | MTR_3g466980 | 45.752 | 153 | 82 | 1 | 4 | 155 | 1 | 153 | 1.68e-38 | 130 |
| MS.gene068870.t1 | MTR_5g075380 | 39.103 | 156 | 94 | 1 | 4 | 158 | 1 | 156 | 4.43e-37 | 125 |
| MS.gene068870.t1 | MTR_4g032620 | 38.065 | 155 | 94 | 2 | 4 | 156 | 1 | 155 | 9.07e-37 | 126 |
| MS.gene068870.t1 | MTR_4g063790 | 40.252 | 159 | 93 | 2 | 4 | 160 | 1 | 159 | 5.78e-35 | 120 |
| MS.gene068870.t1 | MTR_3g466830 | 42.038 | 157 | 90 | 1 | 6 | 161 | 2 | 158 | 1.50e-33 | 116 |
| MS.gene068870.t1 | MTR_4g031910 | 44.667 | 150 | 81 | 2 | 4 | 151 | 1 | 150 | 6.12e-33 | 117 |
| MS.gene068870.t1 | MTR_8g036130 | 40.252 | 159 | 91 | 2 | 4 | 158 | 1 | 159 | 1.41e-32 | 116 |
| MS.gene068870.t1 | MTR_3g466890 | 40.260 | 154 | 91 | 1 | 6 | 158 | 2 | 155 | 1.30e-30 | 108 |
| MS.gene068870.t1 | MTR_4g032260 | 43.750 | 160 | 88 | 2 | 4 | 161 | 1 | 160 | 3.77e-30 | 107 |
| MS.gene068870.t1 | MTR_2g016210 | 42.038 | 157 | 87 | 3 | 4 | 157 | 1 | 156 | 2.96e-29 | 107 |
| MS.gene068870.t1 | MTR_4g032290 | 46.000 | 150 | 79 | 2 | 4 | 151 | 1 | 150 | 5.99e-29 | 106 |
| MS.gene068870.t1 | MTR_3g467080 | 35.625 | 160 | 99 | 3 | 4 | 160 | 1 | 159 | 4.05e-28 | 102 |
| MS.gene068870.t1 | MTR_3g031240 | 34.356 | 163 | 97 | 3 | 4 | 160 | 1 | 159 | 6.11e-28 | 102 |
| MS.gene068870.t1 | MTR_2g035610 | 44.068 | 118 | 65 | 1 | 4 | 120 | 1 | 118 | 6.88e-27 | 98.2 |
| MS.gene068870.t1 | MTR_3g031100 | 35.443 | 158 | 98 | 3 | 4 | 158 | 1 | 157 | 1.06e-26 | 101 |
| MS.gene068870.t1 | MTR_3g465410 | 35.878 | 131 | 82 | 2 | 32 | 160 | 1 | 131 | 4.39e-22 | 86.7 |
| MS.gene068870.t1 | MTR_2g035580 | 32.258 | 155 | 104 | 1 | 4 | 157 | 1 | 155 | 2.24e-21 | 85.1 |
| MS.gene068870.t1 | MTR_3g466900 | 36.800 | 125 | 78 | 1 | 35 | 158 | 2 | 126 | 1.12e-19 | 80.1 |
| MS.gene068870.t1 | MTR_4g028800 | 46.575 | 73 | 37 | 1 | 4 | 76 | 1 | 71 | 7.47e-19 | 76.3 |
| MS.gene068870.t1 | MTR_3g466930 | 35.200 | 125 | 80 | 1 | 35 | 158 | 2 | 126 | 2.84e-18 | 76.3 |
| MS.gene068870.t1 | MTR_4g019670 | 35.833 | 120 | 73 | 2 | 4 | 119 | 1 | 120 | 1.73e-17 | 79.0 |
| MS.gene068870.t1 | MTR_7g106510 | 36.283 | 113 | 68 | 2 | 4 | 112 | 1 | 113 | 1.16e-16 | 76.6 |
| MS.gene068870.t1 | MTR_7g055800 | 33.333 | 117 | 51 | 2 | 4 | 120 | 1 | 90 | 2.94e-15 | 67.4 |
| MS.gene068870.t1 | MTR_7g055790 | 35.714 | 112 | 53 | 2 | 1 | 111 | 56 | 149 | 9.32e-14 | 65.1 |
| MS.gene068870.t1 | MTR_1g033930 | 38.776 | 98 | 59 | 1 | 65 | 161 | 20 | 117 | 2.04e-13 | 63.5 |
| MS.gene068870.t1 | MTR_1g033370 | 41.489 | 94 | 54 | 1 | 67 | 159 | 28 | 121 | 2.27e-13 | 63.5 |
| MS.gene068870.t1 | MTR_1g033790 | 39.796 | 98 | 58 | 1 | 65 | 161 | 20 | 117 | 8.36e-13 | 62.0 |
| MS.gene068870.t1 | MTR_7g055940 | 34.286 | 105 | 42 | 2 | 4 | 108 | 1 | 78 | 9.83e-13 | 60.5 |
| MS.gene068870.t1 | MTR_6g005450 | 31.250 | 112 | 71 | 3 | 4 | 109 | 1 | 112 | 2.41e-12 | 63.9 |
| MS.gene068870.t1 | MTR_3g093900 | 26.623 | 154 | 108 | 2 | 4 | 154 | 4 | 155 | 8.67e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068870.t1 | AT5G48670 | 46.154 | 156 | 83 | 1 | 4 | 158 | 1 | 156 | 5.53e-35 | 124 |
| MS.gene068870.t1 | AT3G05860 | 40.268 | 149 | 88 | 1 | 4 | 151 | 1 | 149 | 2.74e-32 | 114 |
| MS.gene068870.t1 | AT3G05860 | 40.268 | 149 | 88 | 1 | 4 | 151 | 1 | 149 | 1.52e-31 | 114 |
| MS.gene068870.t1 | AT3G05860 | 40.268 | 149 | 88 | 1 | 4 | 151 | 1 | 149 | 2.31e-31 | 113 |
| MS.gene068870.t1 | AT5G26630 | 40.000 | 160 | 95 | 1 | 4 | 162 | 1 | 160 | 5.43e-31 | 111 |
| MS.gene068870.t1 | AT1G65300 | 35.374 | 147 | 94 | 1 | 6 | 151 | 2 | 148 | 7.04e-27 | 102 |
| MS.gene068870.t1 | AT1G65330 | 36.054 | 147 | 93 | 1 | 6 | 151 | 2 | 148 | 2.80e-26 | 100 |
| MS.gene068870.t1 | AT5G26650 | 34.641 | 153 | 99 | 1 | 7 | 158 | 2 | 154 | 4.44e-23 | 94.0 |
| MS.gene068870.t1 | AT5G27960 | 41.026 | 117 | 66 | 2 | 7 | 122 | 2 | 116 | 2.05e-22 | 91.3 |
| MS.gene068870.t1 | AT5G27810 | 43.750 | 96 | 53 | 1 | 32 | 126 | 1 | 96 | 5.30e-21 | 83.2 |
| MS.gene068870.t1 | AT2G28700 | 34.868 | 152 | 92 | 2 | 4 | 151 | 1 | 149 | 2.20e-20 | 85.9 |
| MS.gene068870.t1 | AT1G31630 | 31.429 | 140 | 94 | 2 | 6 | 144 | 2 | 140 | 1.25e-19 | 84.0 |
| MS.gene068870.t1 | AT1G22590 | 32.174 | 115 | 77 | 1 | 4 | 117 | 1 | 115 | 4.11e-19 | 79.3 |
| MS.gene068870.t1 | AT1G31640 | 35.294 | 136 | 86 | 2 | 6 | 140 | 2 | 136 | 3.02e-18 | 80.9 |
| MS.gene068870.t1 | AT5G26580 | 36.975 | 119 | 60 | 2 | 4 | 122 | 1 | 104 | 3.97e-17 | 77.4 |
| MS.gene068870.t1 | AT2G40210 | 38.835 | 103 | 58 | 2 | 4 | 103 | 1 | 101 | 1.81e-13 | 67.0 |
| MS.gene068870.t1 | AT5G58890 | 33.333 | 111 | 66 | 3 | 4 | 107 | 1 | 110 | 3.54e-12 | 63.2 |
| MS.gene068870.t1 | AT5G06500 | 32.877 | 146 | 85 | 5 | 4 | 144 | 1 | 138 | 7.95e-12 | 61.6 |
Find 0 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTAATTGCAAAAAATATGA+AGG | - | 6876:7696-7715 | MS.gene068870:CDS | 20.0% |
| !!! | TGTTTTAGAATCAAAAGGAT+CGG | + | 6876:7425-7444 | MS.gene068870:intergenic | 25.0% |
| TGCTGAATCATTAGTGATAA+AGG | + | 6876:7308-7327 | MS.gene068870:intergenic | 30.0% | |
| ! | TGTTTCTGATTTGAAAGACT+TGG | - | 6876:7669-7688 | MS.gene068870:CDS | 30.0% |
| !! | GAAGAAAAGTATCATCAAGA+AGG | - | 6876:7351-7370 | MS.gene068870:CDS | 30.0% |
| !!! | ACCTCTGTTTTAGAATCAAA+AGG | + | 6876:7430-7449 | MS.gene068870:intergenic | 30.0% |
| !!! | TCCTTTTGATTCTAAAACAG+AGG | - | 6876:7426-7445 | MS.gene068870:CDS | 30.0% |
| AAATCTGAAGGAAGCAAAAC+AGG | - | 6876:7456-7475 | MS.gene068870:CDS | 35.0% | |
| AGAGAACTCACTATTCTTTG+TGG | - | 6876:7376-7395 | MS.gene068870:CDS | 35.0% | |
| AGCTGAACATTCGTATGATT+TGG | - | 6876:7611-7630 | MS.gene068870:CDS | 35.0% | |
| CACTAATGATTCAGCAAGAA+AGG | - | 6876:7312-7331 | MS.gene068870:CDS | 35.0% | |
| TCAGAAACAGTCATATCATC+TGG | + | 6876:7658-7677 | MS.gene068870:intergenic | 35.0% | |
| TGAGAGAAAAAATGTGAACC+AGG | - | 6876:7513-7532 | MS.gene068870:CDS | 35.0% | |
| ! | ACTTTTCTTCCTCTTCTTGT+AGG | + | 6876:7341-7360 | MS.gene068870:intergenic | 35.0% |
| ! | CTTTGGTAATCTGTTGCAAA+AGG | + | 6876:7546-7565 | MS.gene068870:intergenic | 35.0% |
| ! | TGTTTTGCTTCCTTCAGATT+TGG | + | 6876:7457-7476 | MS.gene068870:intergenic | 35.0% |
| !! | TTGATTCTAAAACAGAGGTG+TGG | - | 6876:7431-7450 | MS.gene068870:CDS | 35.0% |
| AAGCAAAACAGGTGATTGAG+AGG | - | 6876:7467-7486 | MS.gene068870:CDS | 40.0% | |
| GCAACAGATTACCAAAGCTA+GGG | - | 6876:7549-7568 | MS.gene068870:CDS | 40.0% | |
| GTTGCAAAAGGAAACTTTCC+TGG | + | 6876:7534-7553 | MS.gene068870:intergenic | 40.0% | |
| TCGGAAATTATAGCACATGC+TGG | + | 6876:7406-7425 | MS.gene068870:intergenic | 40.0% | |
| TGCAACAGATTACCAAAGCT+AGG | - | 6876:7548-7567 | MS.gene068870:CDS | 40.0% | |
| ! | AACAGGTGATTGAGAGGTAT+CGG | - | 6876:7473-7492 | MS.gene068870:CDS | 40.0% |
| ! | GAAGGACATTGATGACAAGA+TGG | - | 6876:7714-7733 | MS.gene068870:CDS | 40.0% |
| GAAAGGCTTCCTACAAGAAG+AGG | - | 6876:7329-7348 | MS.gene068870:CDS | 45.0% | |
| TCTCAGTTGATCCCTAGCTT+TGG | + | 6876:7563-7582 | MS.gene068870:intergenic | 45.0% | |
| TTTCACCTTCTTCCTCGTCA+TGG | + | 6876:7281-7300 | MS.gene068870:intergenic | 45.0% | |
| ! | AGAGGTGTGGCCAAATCTGA+AGG | - | 6876:7444-7463 | MS.gene068870:CDS | 50.0% |
| !!! | CTGGCACTGTGTTGTTTTGC+AGG | + | 6876:7639-7658 | MS.gene068870:intergenic | 50.0% |
| CGAAGCCATGACGAGGAAGA+AGG | - | 6876:7273-7292 | MS.gene068870:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 6876 | gene | 7271 | 7759 | 7271 | ID=MS.gene068870 |
| 6876 | mRNA | 7271 | 7759 | 7271 | ID=MS.gene068870.t1;Parent=MS.gene068870 |
| 6876 | exon | 7271 | 7759 | 7271 | ID=MS.gene068870.t1.exon1;Parent=MS.gene068870.t1 |
| 6876 | CDS | 7271 | 7759 | 7271 | ID=cds.MS.gene068870.t1;Parent=MS.gene068870.t1 |
| Gene Sequence |
| Protein sequence |
