Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068915.t1 | XP_024632337.1 | 84.8 | 151 | 20 | 2 | 1 | 151 | 148 | 295 | 5.10E-61 | 243.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068915.t1 | Q3ECY6 | 52.6 | 133 | 51 | 3 | 12 | 144 | 134 | 254 | 1.1e-24 | 114.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068915.t1 | G7IQD7 | 84.2 | 152 | 20 | 3 | 1 | 151 | 148 | 296 | 9.0e-60 | 239.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene068915.t1 | TR | SET |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068915.t1 | MTR_2g045070 | 84.211 | 152 | 20 | 3 | 1 | 151 | 148 | 296 | 1.06e-80 | 251 |
| MS.gene068915.t1 | MTR_3g062560 | 88.172 | 93 | 10 | 1 | 1 | 93 | 100 | 191 | 1.86e-54 | 170 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068915.t1 | AT1G43245 | 52.632 | 133 | 51 | 3 | 12 | 144 | 134 | 254 | 2.79e-32 | 120 |
| MS.gene068915.t1 | AT1G43245 | 52.632 | 133 | 51 | 3 | 12 | 144 | 134 | 254 | 2.82e-32 | 120 |
Find 27 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATACTCCTCACAATCAATTT+TGG | 0.137975 | 2.1:+45903547 | None:intergenic |
| CAACGACGGTGCAGCGATTC+TGG | 0.215687 | 2.1:-45904769 | MS.gene068915:CDS |
| GAGTATCAAGAGAATTAAAA+AGG | 0.260792 | 2.1:-45903530 | MS.gene068915:CDS |
| GAATGGAACAATGCGAAGTT+TGG | 0.328300 | 2.1:+45904563 | None:intergenic |
| GAGAATAGATAGCAAGCATT+AGG | 0.337383 | 2.1:+45904621 | None:intergenic |
| AGAATAGATAGCAAGCATTA+GGG | 0.355274 | 2.1:+45904622 | None:intergenic |
| CTGATAGTACCAAGCAGAAA+AGG | 0.466570 | 2.1:-45903313 | MS.gene068915:intron |
| CAGAGAAGAAGAAGTGGAGA+AGG | 0.474927 | 2.1:+45904599 | None:intergenic |
| AGAAAGCAGAGAAGAAGAAG+TGG | 0.500998 | 2.1:+45904593 | None:intergenic |
| GATAACGAAGGATGCGCACT+CGG | 0.503690 | 2.1:-45904693 | MS.gene068915:CDS |
| AGTGCTAACCAACGCGGTGG+AGG | 0.554618 | 2.1:-45904721 | MS.gene068915:CDS |
| ATAACGAAGGATGCGCACTC+GGG | 0.558272 | 2.1:-45904692 | MS.gene068915:CDS |
| CAAAGCGGTTGCAGCAACGA+CGG | 0.574084 | 2.1:-45904783 | MS.gene068915:CDS |
| TGTGTTATGCAGATAGATTG+TGG | 0.580846 | 2.1:-45904392 | MS.gene068915:intron |
| TACTGACCTCTTGCAGCCAA+AGG | 0.583268 | 2.1:-45903485 | MS.gene068915:intron |
| TGCATACCTTTGGCTGCAAG+AGG | 0.584254 | 2.1:+45903479 | None:intergenic |
| ATCATGCACCTCCACCGCGT+TGG | 0.596653 | 2.1:+45904713 | None:intergenic |
| GAATAGATAGCAAGCATTAG+GGG | 0.624978 | 2.1:+45904623 | None:intergenic |
| AAAGAAGGACGGGTGATCTG+TGG | 0.627550 | 2.1:-45903571 | MS.gene068915:intron |
| CACTGCACAGAGAGCAACAG+TGG | 0.628872 | 2.1:+45904740 | None:intergenic |
| AGCAGTAGTGAATTTGCACA+AGG | 0.637478 | 2.1:-45904359 | MS.gene068915:intron |
| TGCAGTGCTAACCAACGCGG+TGG | 0.638145 | 2.1:-45904724 | MS.gene068915:CDS |
| CGACGGTGCAGCGATTCTGG+AGG | 0.659267 | 2.1:-45904766 | MS.gene068915:CDS |
| GTGGTCCAAAATTGATTGTG+AGG | 0.672157 | 2.1:-45903552 | MS.gene068915:CDS |
| GTGGAGGTGCATGATAACGA+AGG | 0.699848 | 2.1:-45904705 | MS.gene068915:CDS |
| CTGTGCAGTGCTAACCAACG+CGG | 0.703481 | 2.1:-45904727 | MS.gene068915:CDS |
| GCCGCAGTAGAAAAGCAAAG+CGG | 0.760151 | 2.1:-45904798 | MS.gene068915:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTAAAAATTAATTAATTG+AGG | - | chr2.1:45904500-45904519 | MS.gene068915:intron | 10.0% |
| !! | AAAGAAAAATAAATTACTTG+TGG | + | chr2.1:45903593-45903612 | None:intergenic | 15.0% |
| !! | ATGTTAAACAATTCATATTT+GGG | - | chr2.1:45903837-45903856 | MS.gene068915:intron | 15.0% |
| !! | TATGTTAAACAATTCATATT+TGG | - | chr2.1:45903836-45903855 | MS.gene068915:intron | 15.0% |
| !!! | ACATTTTCTCAAATTATTAT+CGG | + | chr2.1:45903887-45903906 | None:intergenic | 15.0% |
| !!! | TATTATATTTGCTACATAAT+TGG | - | chr2.1:45903800-45903819 | MS.gene068915:intron | 15.0% |
| !! | AGTATCAAGAGAATTAAAAA+GGG | - | chr2.1:45904583-45904602 | MS.gene068915:CDS | 20.0% |
| !! | ATTCTTAATGTATCAAGTTA+GGG | - | chr2.1:45904003-45904022 | MS.gene068915:intron | 20.0% |
| !! | GATGAATTGAGAATAATTTA+GGG | - | chr2.1:45904350-45904369 | MS.gene068915:intron | 20.0% |
| !! | TATTCTTAATGTATCAAGTT+AGG | - | chr2.1:45904002-45904021 | MS.gene068915:intron | 20.0% |
| !! | TGATGAATTGAGAATAATTT+AGG | - | chr2.1:45904349-45904368 | MS.gene068915:intron | 20.0% |
| !!! | TCATTATATGTTTCTAGTTT+AGG | - | chr2.1:45904378-45904397 | MS.gene068915:CDS | 20.0% |
| !!! | TTTTTTTTGCATGAAAAAGA+AGG | - | chr2.1:45904526-45904545 | MS.gene068915:intron | 20.0% |
| ! | AAGCTATAAACTATTTCCAT+AGG | + | chr2.1:45904155-45904174 | None:intergenic | 25.0% |
| ! | ATAAATTTGATGCATACCTT+TGG | + | chr2.1:45904646-45904665 | None:intergenic | 25.0% |
| ! | ATTTATCTTGTGTAATCACA+TGG | - | chr2.1:45903907-45903926 | MS.gene068915:intron | 25.0% |
| ! | CGTCATATAGTTGTATAATT+TGG | + | chr2.1:45904432-45904451 | None:intergenic | 25.0% |
| ! | GAGTATCAAGAGAATTAAAA+AGG | - | chr2.1:45904582-45904601 | MS.gene068915:CDS | 25.0% |
| ! | GTATCAAGAGAATTAAAAAG+GGG | - | chr2.1:45904584-45904603 | MS.gene068915:CDS | 25.0% |
| ! | TATCAAGAGAATTAAAAAGG+GGG | - | chr2.1:45904585-45904604 | MS.gene068915:CDS | 25.0% |
| ! | TTATACATAAGTGCTTATCA+TGG | - | chr2.1:45904222-45904241 | MS.gene068915:intron | 25.0% |
| ! | TTTATCTTGTGTAATCACAT+GGG | - | chr2.1:45903908-45903927 | MS.gene068915:intron | 25.0% |
| !! | TATTTTCATAAGATAGCCTA+TGG | - | chr2.1:45904136-45904155 | MS.gene068915:intron | 25.0% |
| !! | TGATGATATGCTTAGATTTT+TGG | - | chr2.1:45904475-45904494 | MS.gene068915:intron | 25.0% |
| !!! | CACTAGTTACTTTTCAAAAT+AGG | + | chr2.1:45904321-45904340 | None:intergenic | 25.0% |
| !!! | CTATTTTGAAAAGTAACTAG+TGG | - | chr2.1:45904319-45904338 | MS.gene068915:intron | 25.0% |
| !!! | TAAGATACGCTTTTTATTGA+AGG | + | chr2.1:45903967-45903986 | None:intergenic | 25.0% |
| !!! | TGTATTAGTTTTTCATGCAT+TGG | - | chr2.1:45904672-45904691 | MS.gene068915:CDS | 25.0% |
| AAATTAGAGAGTGATATTCG+AGG | - | chr2.1:45904404-45904423 | MS.gene068915:intron | 30.0% | |
| AATAAGCTGTATATCCAAAC+AGG | - | chr2.1:45904269-45904288 | MS.gene068915:intron | 30.0% | |
| AATTAGAGAGTGATATTCGA+GGG | - | chr2.1:45904405-45904424 | MS.gene068915:intron | 30.0% | |
| AGAATAGATAGCAAGCATTA+GGG | + | chr2.1:45903493-45903512 | None:intergenic | 30.0% | |
| AGAGTGGAAATTTAATACCA+GGG | + | chr2.1:45904296-45904315 | None:intergenic | 30.0% | |
| ATAAGCTGTATATCCAAACA+GGG | - | chr2.1:45904270-45904289 | MS.gene068915:intron | 30.0% | |
| TCAAATAAGTCAATCCATGT+AGG | + | chr2.1:45904029-45904048 | None:intergenic | 30.0% | |
| TCGAATATCACTCTCTAATT+TGG | + | chr2.1:45904405-45904424 | None:intergenic | 30.0% | |
| ! | ATACTCCTCACAATCAATTT+TGG | + | chr2.1:45904568-45904587 | None:intergenic | 30.0% |
| ! | TGCTACATAATTGGTTAGTT+AGG | - | chr2.1:45903809-45903828 | MS.gene068915:intron | 30.0% |
| ! | TTTTGCATGAAAAAGAAGGA+CGG | - | chr2.1:45904530-45904549 | MS.gene068915:intron | 30.0% |
| ! | TTTTTGAACATGCTTTCTCT+TGG | - | chr2.1:45903450-45903469 | MS.gene068915:intron | 30.0% |
| !! | TGTTGAGAATTTTGAGTGAA+TGG | + | chr2.1:45903569-45903588 | None:intergenic | 30.0% |
| CTCATAAAACCTATGTCAGT+AGG | + | chr2.1:45904057-45904076 | None:intergenic | 35.0% | |
| GAATAGATAGCAAGCATTAG+GGG | + | chr2.1:45903492-45903511 | None:intergenic | 35.0% | |
| GAGAATAGATAGCAAGCATT+AGG | + | chr2.1:45903494-45903513 | None:intergenic | 35.0% | |
| GAGAGTGGAAATTTAATACC+AGG | + | chr2.1:45904297-45904316 | None:intergenic | 35.0% | |
| TGTGTTATGCAGATAGATTG+TGG | - | chr2.1:45903720-45903739 | MS.gene068915:intron | 35.0% | |
| TTGAGTTTACCTACTGACAT+AGG | - | chr2.1:45904045-45904064 | MS.gene068915:intron | 35.0% | |
| TTTGCATGAAAAAGAAGGAC+GGG | - | chr2.1:45904531-45904550 | MS.gene068915:intron | 35.0% | |
| ! | CAGATAGATTGTGGTGTTTT+TGG | - | chr2.1:45903729-45903748 | MS.gene068915:intron | 35.0% |
| ! | CTTTTCAAAATAGGAGAGAG+TGG | + | chr2.1:45904312-45904331 | None:intergenic | 35.0% |
| AGAAAGCAGAGAAGAAGAAG+TGG | + | chr2.1:45903522-45903541 | None:intergenic | 40.0% | |
| AGCAGTAGTGAATTTGCACA+AGG | - | chr2.1:45903753-45903772 | MS.gene068915:intron | 40.0% | |
| CTGATAGTACCAAGCAGAAA+AGG | - | chr2.1:45904799-45904818 | MS.gene068915:CDS | 40.0% | |
| GAATGGAACAATGCGAAGTT+TGG | + | chr2.1:45903552-45903571 | None:intergenic | 40.0% | |
| GAGAATTAAAAAGGGGGAAG+AGG | - | chr2.1:45904591-45904610 | MS.gene068915:CDS | 40.0% | |
| GATAAACGAACAAGTACGAG+TGG | - | chr2.1:45904707-45904726 | MS.gene068915:CDS | 40.0% | |
| GTGGTCCAAAATTGATTGTG+AGG | - | chr2.1:45904560-45904579 | MS.gene068915:CDS | 40.0% | |
| !! | CACATGGGTTAGTATTGTGT+CGG | - | chr2.1:45903923-45903942 | MS.gene068915:intron | 40.0% |
| CAGAGAAGAAGAAGTGGAGA+AGG | + | chr2.1:45903516-45903535 | None:intergenic | 45.0% | |
| GAGTGGAAGAGGAGAAAGTA+TGG | - | chr2.1:45904724-45904743 | MS.gene068915:CDS | 45.0% | |
| GTATCAAGTTAGGGCCTACA+TGG | - | chr2.1:45904012-45904031 | MS.gene068915:intron | 45.0% | |
| TTTAATACCAGGGCCCTGTT+TGG | + | chr2.1:45904286-45904305 | None:intergenic | 45.0% | |
| !! | ACCGCTTTGCTTTTCTACTG+CGG | + | chr2.1:45903318-45903337 | None:intergenic | 45.0% |
| !! | ATTGTGTCGGTGTCAGACAT+CGG | - | chr2.1:45903936-45903955 | MS.gene068915:intron | 45.0% |
| AAAGAAGGACGGGTGATCTG+TGG | - | chr2.1:45904541-45904560 | MS.gene068915:CDS | 50.0% | |
| ATAACGAAGGATGCGCACTC+GGG | - | chr2.1:45903420-45903439 | MS.gene068915:intron | 50.0% | |
| CGAACAAGTACGAGTGGAAG+AGG | - | chr2.1:45904713-45904732 | MS.gene068915:CDS | 50.0% | |
| GATAACGAAGGATGCGCACT+CGG | - | chr2.1:45903419-45903438 | MS.gene068915:intron | 50.0% | |
| GCCGCAGTAGAAAAGCAAAG+CGG | - | chr2.1:45903314-45903333 | MS.gene068915:intron | 50.0% | |
| TACTGACCTCTTGCAGCCAA+AGG | - | chr2.1:45904627-45904646 | MS.gene068915:CDS | 50.0% | |
| TGCATACCTTTGGCTGCAAG+AGG | + | chr2.1:45904636-45904655 | None:intergenic | 50.0% | |
| TGTATATCCAAACAGGGCCC+TGG | - | chr2.1:45904276-45904295 | MS.gene068915:intron | 50.0% | |
| ! | GTGGAGGTGCATGATAACGA+AGG | - | chr2.1:45903407-45903426 | MS.gene068915:intron | 50.0% |
| !! | TTGTGTCGGTGTCAGACATC+GGG | - | chr2.1:45903937-45903956 | MS.gene068915:intron | 50.0% |
| CAAAGCGGTTGCAGCAACGA+CGG | - | chr2.1:45903329-45903348 | MS.gene068915:intron | 55.0% | |
| CACTGCACAGAGAGCAACAG+TGG | + | chr2.1:45903375-45903394 | None:intergenic | 55.0% | |
| CTGTGCAGTGCTAACCAACG+CGG | - | chr2.1:45903385-45903404 | MS.gene068915:intron | 55.0% | |
| AGTGCTAACCAACGCGGTGG+AGG | - | chr2.1:45903391-45903410 | MS.gene068915:intron | 60.0% | |
| ATCATGCACCTCCACCGCGT+TGG | + | chr2.1:45903402-45903421 | None:intergenic | 60.0% | |
| CAACGACGGTGCAGCGATTC+TGG | - | chr2.1:45903343-45903362 | MS.gene068915:intron | 60.0% | |
| TGCAGTGCTAACCAACGCGG+TGG | - | chr2.1:45903388-45903407 | MS.gene068915:intron | 60.0% | |
| CGACGGTGCAGCGATTCTGG+AGG | - | chr2.1:45903346-45903365 | MS.gene068915:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 45903308 | 45904826 | 45903308 | ID=MS.gene068915 |
| chr2.1 | mRNA | 45903308 | 45904826 | 45903308 | ID=MS.gene068915.t1;Parent=MS.gene068915 |
| chr2.1 | exon | 45904539 | 45904826 | 45904539 | ID=MS.gene068915.t1.exon1;Parent=MS.gene068915.t1 |
| chr2.1 | CDS | 45904539 | 45904826 | 45904539 | ID=cds.MS.gene068915.t1;Parent=MS.gene068915.t1 |
| chr2.1 | exon | 45904360 | 45904402 | 45904360 | ID=MS.gene068915.t1.exon2;Parent=MS.gene068915.t1 |
| chr2.1 | CDS | 45904360 | 45904402 | 45904360 | ID=cds.MS.gene068915.t1;Parent=MS.gene068915.t1 |
| chr2.1 | exon | 45903486 | 45903589 | 45903486 | ID=MS.gene068915.t1.exon3;Parent=MS.gene068915.t1 |
| chr2.1 | CDS | 45903486 | 45903589 | 45903486 | ID=cds.MS.gene068915.t1;Parent=MS.gene068915.t1 |
| chr2.1 | exon | 45903308 | 45903328 | 45903308 | ID=MS.gene068915.t1.exon4;Parent=MS.gene068915.t1 |
| chr2.1 | CDS | 45903308 | 45903328 | 45903308 | ID=cds.MS.gene068915.t1;Parent=MS.gene068915.t1 |
| Gene Sequence |
| Protein sequence |
