AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068940.t1 MTR_6g444980 65.464 388 78 18 1 345 1 375 1.30e-147 421
MS.gene068940.t1 MTR_6g444980 60.677 384 76 18 1 345 1 348 3.45e-129 373
MS.gene068940.t1 MTR_5g027440 45.798 238 92 11 13 219 1 232 1.05e-51 173
MS.gene068940.t1 MTR_2g086450 52.941 170 63 2 26 178 35 204 9.44e-51 174
MS.gene068940.t1 MTR_4g081710 55.195 154 61 1 29 174 10 163 1.71e-50 171
MS.gene068940.t1 MTR_6g032990 46.190 210 91 7 29 221 35 239 4.57e-50 172
MS.gene068940.t1 MTR_5g041350 55.414 157 61 2 29 176 8 164 3.97e-49 168
MS.gene068940.t1 MTR_2g027800 53.529 170 67 4 24 183 27 194 1.26e-46 160
MS.gene068940.t1 MTR_1g090670 52.381 168 67 4 15 173 23 186 2.11e-45 157
MS.gene068940.t1 MTR_7g088070 54.545 154 54 5 36 182 50 194 1.10e-44 154
MS.gene068940.t1 MTR_2g027860 59.398 133 51 2 36 167 49 179 8.83e-44 154
MS.gene068940.t1 MTR_7g088070 53.247 154 52 4 36 182 50 190 2.61e-43 151
MS.gene068940.t1 MTR_2g027800 47.191 178 52 4 24 183 27 180 1.27e-40 144
MS.gene068940.t1 MTR_7g098250 38.164 207 109 6 36 236 262 455 3.54e-36 137
MS.gene068940.t1 MTR_1g080330 40.237 169 85 4 2 167 148 303 6.29e-34 129
MS.gene068940.t1 MTR_7g115530 41.722 151 77 3 36 183 218 360 7.78e-34 129
MS.gene068940.t1 MTR_1g053835 42.657 143 71 3 32 171 254 388 6.31e-30 119
MS.gene068940.t1 MTR_7g093030 40.714 140 75 3 36 173 212 345 6.29e-29 115
MS.gene068940.t1 MTR_1g080330 39.865 148 73 4 2 146 148 282 1.97e-26 106
MS.gene068940.t1 MTR_7g115530 44.737 114 54 2 36 146 218 325 3.98e-26 106
MS.gene068940.t1 MTR_7g068600 43.182 132 69 2 36 164 13 141 2.93e-25 102
MS.gene068940.t1 MTR_1g053800 39.103 156 83 2 26 177 167 314 5.10e-25 103
MS.gene068940.t1 MTR_7g115530 63.158 57 21 0 36 92 218 274 8.63e-20 88.6
MS.gene068940.t1 MTR_7g115530 63.158 57 21 0 36 92 218 274 3.42e-19 87.8
MS.gene068940.t1 MTR_1g090670 46.087 115 49 4 68 173 1 111 4.49e-19 85.1
MS.gene068940.t1 MTR_7g115530 66.038 53 18 0 36 88 218 270 3.29e-18 83.6
MS.gene068940.t1 MTR_7g093030 57.895 57 24 0 36 92 212 268 5.61e-18 83.6
MS.gene068940.t1 MTR_7g115530 66.667 51 17 0 36 86 218 268 2.85e-17 81.3
MS.gene068940.t1 MTR_2g023580 50.000 78 37 1 37 112 56 133 8.54e-17 79.0
MS.gene068940.t1 MTR_3g450310 36.364 132 62 3 36 158 19 137 3.92e-16 76.6
MS.gene068940.t1 MTR_7g069660 55.357 56 25 0 37 92 72 127 1.69e-15 77.0
MS.gene068940.t1 MTR_3g064840 51.429 70 34 0 37 106 275 344 2.00e-15 77.4
MS.gene068940.t1 MTR_6g071625 56.667 60 26 0 37 96 203 262 2.23e-15 76.6
MS.gene068940.t1 MTR_1g112370 56.452 62 22 1 37 93 23 84 2.72e-15 76.3
MS.gene068940.t1 MTR_0223s0040 62.264 53 20 0 37 89 19 71 3.79e-15 75.1
MS.gene068940.t1 MTR_8g066310 58.929 56 23 0 37 92 174 229 6.25e-15 75.5
MS.gene068940.t1 MTR_5g029470 58.929 56 23 0 37 92 220 275 7.82e-15 75.5
MS.gene068940.t1 MTR_1g053830 49.091 55 28 0 38 92 20 74 1.07e-14 69.7
MS.gene068940.t1 MTR_1g021520 57.143 56 24 0 37 92 127 182 1.62e-14 73.6
MS.gene068940.t1 MTR_5g041350 71.739 46 13 0 131 176 8 53 1.88e-14 72.4
MS.gene068940.t1 MTR_8g086410 53.571 56 26 0 37 92 112 167 2.23e-14 73.9
MS.gene068940.t1 MTR_8g086410 53.571 56 26 0 37 92 110 165 2.25e-14 73.9
MS.gene068940.t1 MTR_5g041350 71.739 46 13 0 131 176 30 75 3.20e-14 72.0
MS.gene068940.t1 MTR_2g016220 55.357 56 25 0 37 92 90 145 3.25e-14 73.2
MS.gene068940.t1 MTR_2g016220 55.357 56 25 0 37 92 90 145 3.33e-14 73.2
MS.gene068940.t1 MTR_2g084230 55.357 56 25 0 37 92 98 153 4.11e-14 72.4
MS.gene068940.t1 MTR_2g084230 55.357 56 25 0 37 92 98 153 4.16e-14 73.2
MS.gene068940.t1 MTR_7g093010 37.500 128 68 4 36 158 213 333 4.43e-14 72.8
MS.gene068940.t1 MTR_4g121020 59.322 59 23 1 32 90 107 164 1.15e-13 72.4
MS.gene068940.t1 MTR_4g121020 59.322 59 23 1 32 90 211 268 1.39e-13 72.0
MS.gene068940.t1 MTR_4g121020 59.322 59 23 1 32 90 211 268 1.39e-13 72.0
MS.gene068940.t1 MTR_4g064730 53.623 69 30 2 23 90 117 184 2.82e-13 70.1
MS.gene068940.t1 MTR_8g086410 56.000 50 22 0 37 86 112 161 3.20e-13 70.5
MS.gene068940.t1 MTR_1g032570 61.818 55 20 1 36 90 131 184 3.46e-13 70.9
MS.gene068940.t1 MTR_2g016220 58.000 50 21 0 37 86 90 139 3.84e-13 70.1
MS.gene068940.t1 MTR_1g032570 61.818 55 20 1 36 90 200 253 4.29e-13 70.5
MS.gene068940.t1 MTR_7g089010 61.818 55 20 1 36 90 91 144 5.46e-13 68.9
MS.gene068940.t1 MTR_1g093080 60.000 55 22 0 36 90 211 265 9.54e-13 68.9
MS.gene068940.t1 MTR_2g034960 54.237 59 26 1 32 90 207 264 2.02e-12 68.6
MS.gene068940.t1 MTR_5g017980 60.000 55 22 0 36 90 193 247 2.50e-12 67.4
MS.gene068940.t1 MTR_5g054300 58.182 55 23 0 36 90 198 252 9.81e-12 65.9
MS.gene068940.t1 MTR_4g021790 52.542 59 27 1 32 90 164 221 1.01e-11 65.1
MS.gene068940.t1 MTR_4g086835 55.172 58 26 0 33 90 271 328 3.97e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068940.t1 AT1G79430 54.247 365 137 9 4 345 1 358 9.88e-115 337
MS.gene068940.t1 AT1G79430 47.333 300 129 8 68 345 1 293 7.41e-71 223
MS.gene068940.t1 AT3G12730 61.453 179 60 3 25 194 12 190 3.32e-67 211
MS.gene068940.t1 AT4G13640 46.097 269 106 6 26 269 27 281 4.57e-64 205
MS.gene068940.t1 AT3G24120 68.790 157 32 4 25 168 30 182 7.69e-63 202
MS.gene068940.t1 AT4G13640 45.588 272 106 7 26 269 27 284 5.45e-62 200
MS.gene068940.t1 AT3G24120 67.500 160 32 5 25 168 30 185 1.19e-60 197
MS.gene068940.t1 AT5G18240 54.082 196 70 6 25 207 34 222 3.27e-56 188
MS.gene068940.t1 AT5G18240 54.082 196 70 6 25 207 34 222 3.27e-56 188
MS.gene068940.t1 AT3G04030 58.580 169 58 3 25 181 34 202 1.06e-55 187
MS.gene068940.t1 AT5G18240 53.061 196 70 7 25 207 34 220 8.85e-53 179
MS.gene068940.t1 AT3G04030 57.396 169 59 4 25 181 34 201 1.46e-52 178
MS.gene068940.t1 AT5G18240 51.531 196 69 7 25 207 34 216 1.84e-49 170
MS.gene068940.t1 AT5G18240 51.531 196 69 7 25 207 34 216 1.84e-49 170
MS.gene068940.t1 AT3G04030 55.621 169 57 4 25 181 34 196 4.47e-49 169
MS.gene068940.t1 AT1G69580 49.412 170 72 2 29 184 27 196 1.44e-47 164
MS.gene068940.t1 AT1G69580 49.123 171 72 2 29 184 27 197 2.17e-47 163
MS.gene068940.t1 AT5G45580 41.629 221 103 6 26 220 13 233 1.11e-45 157
MS.gene068940.t1 AT5G45580 41.935 217 100 6 30 220 1 217 9.39e-45 154
MS.gene068940.t1 AT2G01060 58.333 132 53 1 36 167 15 144 3.38e-44 154
MS.gene068940.t1 AT3G12730 53.676 136 54 3 68 194 1 136 4.70e-37 132
MS.gene068940.t1 AT4G28610 39.881 168 91 3 36 199 225 386 7.71e-34 129
MS.gene068940.t1 AT3G13040 44.172 163 76 5 22 171 218 378 9.76e-34 129
MS.gene068940.t1 AT3G13040 44.172 163 76 5 22 171 218 378 9.76e-34 129
MS.gene068940.t1 AT3G13040 44.172 163 76 5 22 171 218 378 9.76e-34 129
MS.gene068940.t1 AT5G29000 41.290 155 88 2 13 167 168 319 9.13e-32 122
MS.gene068940.t1 AT5G29000 41.290 155 88 2 13 167 168 319 9.13e-32 122
MS.gene068940.t1 AT5G29000 41.290 155 88 2 13 167 211 362 1.56e-31 123
MS.gene068940.t1 AT5G06800 45.522 134 70 2 36 167 192 324 3.96e-30 118
MS.gene068940.t1 AT3G04450 42.963 135 71 2 36 167 198 329 4.04e-30 119
MS.gene068940.t1 AT3G04450 42.963 135 71 2 36 167 198 329 4.04e-30 119
MS.gene068940.t1 AT5G06800 46.617 133 65 3 36 167 192 319 4.87e-30 118
MS.gene068940.t1 AT3G04450 42.963 135 71 2 36 167 238 369 5.10e-30 119
MS.gene068940.t1 AT3G04030 52.381 126 48 3 68 181 1 126 1.43e-28 113
MS.gene068940.t1 AT2G20400 39.103 156 93 1 36 191 231 384 1.23e-27 112
MS.gene068940.t1 AT2G20400 38.365 159 89 2 36 191 231 383 1.29e-27 112
MS.gene068940.t1 AT5G18240 43.791 153 60 7 68 207 1 140 5.74e-23 97.8
MS.gene068940.t1 AT3G04030 48.413 126 47 4 68 181 1 120 2.53e-22 95.9
MS.gene068940.t1 AT2G01060 49.485 97 47 1 71 167 1 95 4.52e-21 90.9
MS.gene068940.t1 AT2G06020 59.375 64 26 0 29 92 79 142 7.01e-21 91.7
MS.gene068940.t1 AT5G06800 59.649 57 23 0 36 92 192 248 5.36e-18 83.2
MS.gene068940.t1 AT5G29000 48.750 80 38 2 13 92 211 287 1.91e-17 82.0
MS.gene068940.t1 AT3G04450 56.140 57 25 0 36 92 238 294 3.45e-17 81.6
MS.gene068940.t1 AT1G14600 50.685 73 31 1 22 89 4 76 1.75e-16 78.6
MS.gene068940.t1 AT2G40260 60.714 56 22 0 37 92 83 138 4.35e-16 79.3
MS.gene068940.t1 AT2G42660 58.929 56 23 0 37 92 51 106 5.75e-16 77.0
MS.gene068940.t1 AT4G04605 58.491 53 22 0 37 89 15 67 6.43e-16 76.3
MS.gene068940.t1 AT2G02060 57.627 59 25 0 35 93 28 86 1.50e-15 75.9
MS.gene068940.t1 AT2G02060 57.627 59 25 0 35 93 28 86 1.50e-15 75.9
MS.gene068940.t1 AT1G32240 56.667 60 26 0 37 96 214 273 5.11e-15 75.9
MS.gene068940.t1 AT5G16560 56.667 60 26 0 37 96 220 279 5.53e-15 75.9
MS.gene068940.t1 AT1G32240 58.929 56 23 0 37 92 214 269 6.50e-15 75.5
MS.gene068940.t1 AT2G38300 57.143 56 24 0 37 92 55 110 7.38e-15 75.1
MS.gene068940.t1 AT5G42630 57.143 56 24 0 37 92 106 161 1.36e-14 72.4
MS.gene068940.t1 AT5G42630 57.143 56 24 0 37 92 106 161 2.06e-14 72.8
MS.gene068940.t1 AT4G17695 42.857 84 45 1 37 117 165 248 2.44e-14 73.2
MS.gene068940.t1 AT3G46640 55.714 70 27 2 24 90 129 197 4.29e-14 72.8
MS.gene068940.t1 AT3G46640 55.714 70 27 2 24 90 129 197 4.29e-14 72.8
MS.gene068940.t1 AT3G46640 55.714 70 27 2 24 90 129 197 5.52e-14 72.4
MS.gene068940.t1 AT5G59570 54.286 70 28 2 24 90 126 194 8.76e-14 71.2
MS.gene068940.t1 AT5G59570 54.286 70 28 2 24 90 126 194 8.76e-14 71.2
MS.gene068940.t1 AT5G05090 61.818 55 20 1 36 90 81 134 1.66e-13 70.1
MS.gene068940.t1 AT3G10760 61.818 55 20 1 36 90 105 158 2.55e-13 70.5
MS.gene068940.t1 AT4G04580 47.059 68 33 1 37 104 15 79 4.69e-13 67.0
MS.gene068940.t1 AT2G40970 60.000 55 21 1 36 90 105 158 1.03e-12 67.8
MS.gene068940.t1 AT4G37180 55.172 58 26 0 38 95 212 269 1.04e-12 68.9
MS.gene068940.t1 AT4G37180 55.172 58 26 0 38 95 219 276 1.12e-12 68.6
MS.gene068940.t1 AT5G58080 31.481 162 99 5 33 190 191 344 1.43e-12 68.9
MS.gene068940.t1 AT3G25790 60.000 50 20 0 41 90 201 250 4.30e-12 67.0
MS.gene068940.t1 AT3G16857 56.364 55 23 1 36 90 167 220 9.56e-12 66.6
MS.gene068940.t1 AT1G49560 52.941 68 31 1 36 102 192 259 1.02e-11 65.5
MS.gene068940.t1 AT3G16857 56.364 55 23 1 36 90 237 290 1.13e-11 66.2
MS.gene068940.t1 AT3G16857 56.364 55 23 1 36 90 237 290 1.20e-11 66.2
MS.gene068940.t1 AT2G01760 54.545 55 24 1 36 90 135 188 2.19e-11 64.3
MS.gene068940.t1 AT5G44190 55.556 54 23 1 36 89 147 199 5.18e-11 63.9
MS.gene068940.t1 AT5G44190 55.556 54 23 1 36 89 147 199 6.44e-11 63.2
MS.gene068940.t1 AT1G13300 54.545 55 25 0 36 90 181 235 6.75e-11 63.2

Find 78 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTTCCACTTCCATTAAA+AGG 0.118507 8.1:-58413762 None:intergenic
TCATTATCATTCCAAATTAT+TGG 0.120463 8.1:-58413786 None:intergenic
AATGATTCACCTTTGAAATT+AGG 0.181239 8.1:-58413864 None:intergenic
TGATCTTAGGCTTCATGATT+TGG 0.243321 8.1:+58413806 MS.gene068940:CDS
ATCATAGTTGAGGGCTTCTT+TGG 0.252047 8.1:-58411582 None:intergenic
GTGGAAAGAGTCCAATAATT+TGG 0.261771 8.1:+58413775 MS.gene068940:CDS
GTCCATCGAAGCCGAGGTTT+TGG 0.292562 8.1:-58411666 None:intergenic
TTTGGAATGATAATGATCTT+AGG 0.294293 8.1:+58413793 MS.gene068940:CDS
AAAATACATGCAAAGTATAT+TGG 0.312337 8.1:+58413470 MS.gene068940:CDS
AATGATGAAAATTTGTTTGT+TGG 0.313875 8.1:+58413723 MS.gene068940:CDS
ATTCAATGAGCAATCTATTA+AGG 0.315644 8.1:+58412302 MS.gene068940:CDS
CCACCTTAAGAGCCATCTTC+AGG 0.346223 8.1:+58412113 MS.gene068940:CDS
GATCTTAGGCTTCATGATTT+GGG 0.360986 8.1:+58413807 MS.gene068940:CDS
AAATGAGGAAAATTATTTAG+AGG 0.363464 8.1:-58413600 None:intergenic
TTTGAACTGCAAAGAAATAC+TGG 0.365692 8.1:+58412456 MS.gene068940:CDS
TAATTTCAAAGGTGAATCAT+TGG 0.367186 8.1:+58413866 MS.gene068940:CDS
GAAACAAATTCTTTCATGTC+AGG 0.374130 8.1:-58413576 None:intergenic
AAAGCTTATCACACACTTGC+AGG 0.378431 8.1:+58413495 MS.gene068940:CDS
ATAGAGAGGCCAACCCTTGA+TGG 0.379955 8.1:+58413678 MS.gene068940:CDS
AAAACAATCATGAGGGTTAT+GGG 0.394376 8.1:+58412069 MS.gene068940:CDS
GTTGATGCTGTTGCTCAGCT+TGG 0.397802 8.1:+58411711 MS.gene068940:CDS
AAACCTGAAGATGGCTCTTA+AGG 0.399926 8.1:-58412116 None:intergenic
TTTCAAGATCTTAACATTTG+TGG 0.407965 8.1:+58413621 MS.gene068940:CDS
TTGCTCATTGAATTCCTTGT+GGG 0.416474 8.1:-58412292 None:intergenic
ACAAATTTCAAGGGTATGAA+TGG 0.427658 8.1:+58413540 MS.gene068940:CDS
ATTTGTTTGTTGGAAAGAAA+AGG 0.433652 8.1:+58413733 MS.gene068940:CDS
GAGCCTCAATCCTCAATTGA+AGG 0.444605 8.1:-58413443 None:intergenic
AGCTGCAGCTACAAATTTCA+AGG 0.446327 8.1:+58413530 MS.gene068940:CDS
TTTGATCCATCTGTGAAGCT+TGG 0.458634 8.1:+58413951 MS.gene068940:CDS
GGTAGAAGCTCATCACCATT+TGG 0.458779 8.1:+58414008 MS.gene068940:CDS
TTCAAAAGCACCTTCAATTG+AGG 0.460223 8.1:+58413433 MS.gene068940:CDS
GCTGCAACAGCATTGATCAT+AGG 0.461064 8.1:-58413984 None:intergenic
GATGCTGTTGCTCAGCTTGG+AGG 0.468617 8.1:+58411714 MS.gene068940:CDS
AATTAGTATTCTCAACCAAA+TGG 0.471739 8.1:-58414023 None:intergenic
TCTTTCCACTTCCATTAAAA+GGG 0.471840 8.1:-58413761 None:intergenic
GATGGCTCTTAAGGTGGTAA+AGG 0.478682 8.1:-58412107 None:intergenic
TTGAAATTAGGAACATCATC+AGG 0.480806 8.1:-58413852 None:intergenic
TGAGAATTCATCATAGTTGA+GGG 0.480954 8.1:-58411591 None:intergenic
ATAGTTGAGGGCTTCTTTGG+AGG 0.483070 8.1:-58411579 None:intergenic
ATCCAAAACCTCGGCTTCGA+TGG 0.484201 8.1:+58411664 MS.gene068940:CDS
TAGGCATTATAATTCCATCA+AGG 0.485394 8.1:-58413692 None:intergenic
ATGTGTGTTCAAGGTGACTC+TGG 0.488847 8.1:+58411624 MS.gene068940:CDS
GATAGACCCATGTGTGTTCA+AGG 0.494833 8.1:+58411615 MS.gene068940:CDS
CATTAATAGAAATTTAGGCT+TGG 0.499746 8.1:+58412261 MS.gene068940:intron
AGGCATTATAATTCCATCAA+GGG 0.502907 8.1:-58413691 None:intergenic
TAGTTGAGGGCTTCTTTGGA+GGG 0.504455 8.1:-58411578 None:intergenic
TTTGTGTTCAAACCTGAAGA+TGG 0.506495 8.1:-58412125 None:intergenic
GCACCTTCAATTGAGGATTG+AGG 0.506889 8.1:+58413440 MS.gene068940:CDS
GATGATGTTCCTAATTTCAA+AGG 0.507643 8.1:+58413855 MS.gene068940:CDS
ATGGCTCTTAAGGTGGTAAA+GGG 0.519319 8.1:-58412106 None:intergenic
ATTATAATTCCATCAAGGGT+TGG 0.522631 8.1:-58413687 None:intergenic
ATGATCAATGCTGTTGCAGC+TGG 0.528955 8.1:+58413987 MS.gene068940:CDS
GGCACCTCGTCTTCTATGAC+TGG 0.530737 8.1:+58412477 MS.gene068940:CDS
CACACTTGCAGGTGAAAACA+TGG 0.531497 8.1:+58413506 MS.gene068940:CDS
ATGTTAAGATCTTGAAAATG+AGG 0.534145 8.1:-58413615 None:intergenic
ATGAGAATTCATCATAGTTG+AGG 0.541419 8.1:-58411592 None:intergenic
TTGGATCAGTGGTGAGGACA+AGG 0.541614 8.1:-58411647 None:intergenic
CATGAGGGTTATGGGTGTGA+AGG 0.546666 8.1:+58412077 MS.gene068940:CDS
AAACAAATTCTTTCATGTCA+GGG 0.546749 8.1:-58413575 None:intergenic
GTTATTCCAAGCTTCACAGA+TGG 0.570892 8.1:-58413957 None:intergenic
AATGAGCAATCTATTAAGGA+TGG 0.585044 8.1:+58412306 MS.gene068940:CDS
ATTGCTCATTGAATTCCTTG+TGG 0.590023 8.1:-58412293 None:intergenic
ATGAGGGTTATGGGTGTGAA+GGG 0.590465 8.1:+58412078 MS.gene068940:CDS
CCTGAAGATGGCTCTTAAGG+TGG 0.594196 8.1:-58412113 None:intergenic
TCGACGGTCCATCGAAGCCG+AGG 0.598315 8.1:-58411672 None:intergenic
AAGGGTATGAATGGAACTCA+AGG 0.599882 8.1:+58413549 MS.gene068940:CDS
AGAGTCACCTTGAACACACA+TGG 0.606753 8.1:-58411622 None:intergenic
TGCTCATTGAATTCCTTGTG+GGG 0.607189 8.1:-58412291 None:intergenic
ATCTTCATCAAAACATAGAG+AGG 0.609398 8.1:+58413664 MS.gene068940:CDS
TCTATTAAGGATGGTATGAG+AGG 0.614631 8.1:+58412315 MS.gene068940:CDS
GCTTGGAAAGCAACCCCACA+AGG 0.615422 8.1:+58412278 MS.gene068940:CDS
CAATTGAGGATTGAGGCTCA+AGG 0.648335 8.1:+58413447 MS.gene068940:CDS
GCTGCAGCTACAAATTTCAA+GGG 0.661788 8.1:+58413531 MS.gene068940:CDS
AAGACTGCATGAACAGCTAG+AGG 0.664015 8.1:+58412962 MS.gene068940:CDS
AAAGCGTTCATGAAGTTCGA+CGG 0.675081 8.1:-58411688 None:intergenic
TCACCACTGATCCAAAACCT+CGG 0.679105 8.1:+58411655 MS.gene068940:CDS
GCGACCAGTCATAGAAGACG+AGG 0.697663 8.1:-58412481 None:intergenic
GAGTCACCTTGAACACACAT+GGG 0.714712 8.1:-58411621 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTAAATTAAAATGC+AGG - chr8.1:58412762-58412781 None:intergenic 10.0%
!! TAAATAAATTAAGAATCAAA+AGG - chr8.1:58412533-58412552 None:intergenic 10.0%
!! TTAGAAAATAAAAATTCTTA+AGG - chr8.1:58412363-58412382 None:intergenic 10.0%
!!! TTTTCATCATTATTATTATT+AGG - chr8.1:58413714-58413733 None:intergenic 10.0%
!! AAAAGAAAGAAAAGAATAAA+AGG - chr8.1:58411761-58411780 None:intergenic 15.0%
!! ATAAAATCAAAGAACTTAAA+AGG - chr8.1:58412232-58412251 None:intergenic 15.0%
!! TAAAATCAAAGAACTTAAAA+GGG - chr8.1:58412231-58412250 None:intergenic 15.0%
!!! CAATAAAAAATTTTACGATT+TGG + chr8.1:58412796-58412815 MS.gene068940:intron 15.0%
!!! TTTTATTCTTTTCTTTCTTT+TGG + chr8.1:58411760-58411779 MS.gene068940:intron 15.0%
!!! TTTTGCATTAATAGAAATTT+AGG + chr8.1:58412256-58412275 MS.gene068940:intron 15.0%
!!! TTTTTGTTTTTGTTTTTTGA+GGG - chr8.1:58411908-58411927 None:intergenic 15.0%
!!! TTTTTTGTTTTTGTTTTTTG+AGG - chr8.1:58411909-58411928 None:intergenic 15.0%
!! AAAATACATGCAAAGTATAT+TGG + chr8.1:58413470-58413489 MS.gene068940:CDS 20.0%
!! AAATGAGGAAAATTATTTAG+AGG - chr8.1:58413603-58413622 None:intergenic 20.0%
!! CATATTATAATGAGAAACTT+TGG + chr8.1:58412835-58412854 MS.gene068940:intron 20.0%
!! TAAATTTCTTTACCTCATTT+AGG + chr8.1:58412163-58412182 MS.gene068940:intron 20.0%
!! TCATTATCATTCCAAATTAT+TGG - chr8.1:58413789-58413808 None:intergenic 20.0%
!!! AAAGTTAGTTTCTAATTTCT+AGG + chr8.1:58413113-58413132 MS.gene068940:intron 20.0%
!!! AATGATGAAAATTTGTTTGT+TGG + chr8.1:58413723-58413742 MS.gene068940:CDS 20.0%
!!! TTAATTTGTAGGTTTATTTG+TGG + chr8.1:58413406-58413425 MS.gene068940:intron 20.0%
!!! TTTCAATATTAGTTTACTTG+TGG - chr8.1:58412569-58412588 None:intergenic 20.0%
! AAACAAATTCTTTCATGTCA+GGG - chr8.1:58413578-58413597 None:intergenic 25.0%
! AAATAACAAAGTGATTGAGT+TGG + chr8.1:58413211-58413230 MS.gene068940:intron 25.0%
! AGTTAGTAGTTATTATATGC+AGG - chr8.1:58412718-58412737 None:intergenic 25.0%
! ATGTTAAGATCTTGAAAATG+AGG - chr8.1:58413618-58413637 None:intergenic 25.0%
! ATTCAATGAGCAATCTATTA+AGG + chr8.1:58412302-58412321 MS.gene068940:CDS 25.0%
! CATTAATAGAAATTTAGGCT+TGG + chr8.1:58412261-58412280 MS.gene068940:intron 25.0%
! GATTACTAAAATTTCTCTCT+AGG + chr8.1:58413338-58413357 MS.gene068940:intron 25.0%
! GGATTAAGACAAAAAATATG+TGG + chr8.1:58413359-58413378 MS.gene068940:intron 25.0%
! TAATCTATAGTTCAATCAGA+AGG - chr8.1:58413323-58413342 None:intergenic 25.0%
! TAATTTCAAAGGTGAATCAT+TGG + chr8.1:58413866-58413885 MS.gene068940:CDS 25.0%
! TTTCAAGAAAGATTTGAACT+CGG - chr8.1:58413276-58413295 None:intergenic 25.0%
! TTTCAAGATCTTAACATTTG+TGG + chr8.1:58413621-58413640 MS.gene068940:CDS 25.0%
!! AGACTAAAAGTATATAGAAG+AGG - chr8.1:58412606-58412625 None:intergenic 25.0%
!! ATTTGTTTGTTGGAAAGAAA+AGG + chr8.1:58413733-58413752 MS.gene068940:CDS 25.0%
!! GACTAAAAGTATATAGAAGA+GGG - chr8.1:58412605-58412624 None:intergenic 25.0%
!! TATATAGAGCTAGTGTTTTA+TGG + chr8.1:58413011-58413030 MS.gene068940:intron 25.0%
!! TTTGGAATGATAATGATCTT+AGG + chr8.1:58413793-58413812 MS.gene068940:CDS 25.0%
!!! AATGATTCACCTTTGAAATT+AGG - chr8.1:58413867-58413886 None:intergenic 25.0%
!!! TTTTGTTTTCATGACAGTTT+CGG + chr8.1:58412431-58412450 MS.gene068940:intron 25.0%
AAAAACAATCATGAGGGTTA+TGG + chr8.1:58412068-58412087 MS.gene068940:CDS 30.0%
AAAACAATCATGAGGGTTAT+GGG + chr8.1:58412069-58412088 MS.gene068940:CDS 30.0%
AACCGTAACTAAAACTTTGT+TGG + chr8.1:58412902-58412921 MS.gene068940:intron 30.0%
AATGAGCAATCTATTAAGGA+TGG + chr8.1:58412306-58412325 MS.gene068940:CDS 30.0%
ACACTATACATATGATCTTG+TGG - chr8.1:58413037-58413056 None:intergenic 30.0%
AGATCATATGTATAGTGTTC+AGG + chr8.1:58413039-58413058 MS.gene068940:intron 30.0%
AGGCATTATAATTCCATCAA+GGG - chr8.1:58413694-58413713 None:intergenic 30.0%
ATCTTCATCAAAACATAGAG+AGG + chr8.1:58413664-58413683 MS.gene068940:CDS 30.0%
ATGAGAATTCATCATAGTTG+AGG - chr8.1:58411595-58411614 None:intergenic 30.0%
ATTATAATTCCATCAAGGGT+TGG - chr8.1:58413690-58413709 None:intergenic 30.0%
GAAACAAATTCTTTCATGTC+AGG - chr8.1:58413579-58413598 None:intergenic 30.0%
GATGATGTTCCTAATTTCAA+AGG + chr8.1:58413855-58413874 MS.gene068940:CDS 30.0%
TAGGCATTATAATTCCATCA+AGG - chr8.1:58413695-58413714 None:intergenic 30.0%
TCAAAGAACTTAAAAGGGAA+AGG - chr8.1:58412226-58412245 None:intergenic 30.0%
TCTACAAATTAACACACAAG+AGG - chr8.1:58412039-58412058 None:intergenic 30.0%
TCTTTCCACTTCCATTAAAA+GGG - chr8.1:58413764-58413783 None:intergenic 30.0%
TGAGAATTCATCATAGTTGA+GGG - chr8.1:58411594-58411613 None:intergenic 30.0%
TTAGTTACGGTTACATACAA+TGG - chr8.1:58412894-58412913 None:intergenic 30.0%
TTGAAATTAGGAACATCATC+AGG - chr8.1:58413855-58413874 None:intergenic 30.0%
TTTGAACTGCAAAGAAATAC+TGG + chr8.1:58412456-58412475 MS.gene068940:CDS 30.0%
! AAAAGGACTAACCCTTTTAA+TGG + chr8.1:58413750-58413769 MS.gene068940:CDS 30.0%
! ACAAATTTCAAGGGTATGAA+TGG + chr8.1:58413540-58413559 MS.gene068940:CDS 30.0%
! ATAACCCTCATGATTGTTTT+TGG - chr8.1:58412069-58412088 None:intergenic 30.0%
! ATTAGTTTACTTGTGGTTGA+GGG - chr8.1:58412562-58412581 None:intergenic 30.0%
! TATTAGTTTACTTGTGGTTG+AGG - chr8.1:58412563-58412582 None:intergenic 30.0%
!! TTGGTTTGTAAACTTCACTA+AGG + chr8.1:58413230-58413249 MS.gene068940:intron 30.0%
!!! CACCAACAAAGTTTTAGTTA+CGG - chr8.1:58412907-58412926 None:intergenic 30.0%
ATGCAGGAAAATACATGTTG+AGG - chr8.1:58412746-58412765 None:intergenic 35.0%
ATTGCTCATTGAATTCCTTG+TGG - chr8.1:58412296-58412315 None:intergenic 35.0%
CACAAAAGTGAACCTAAATG+AGG - chr8.1:58412178-58412197 None:intergenic 35.0%
CACTCCAAAAACAATCATGA+GGG + chr8.1:58412062-58412081 MS.gene068940:CDS 35.0%
CGTAACTAAAACTTTGTTGG+TGG + chr8.1:58412905-58412924 MS.gene068940:intron 35.0%
CTCTTTCCACTTCCATTAAA+AGG - chr8.1:58413765-58413784 None:intergenic 35.0%
GATCTTAGGCTTCATGATTT+GGG + chr8.1:58413807-58413826 MS.gene068940:CDS 35.0%
GTGCAAACTACATAACTAGA+AGG + chr8.1:58411782-58411801 MS.gene068940:intron 35.0%
GTGGAAAGAGTCCAATAATT+TGG + chr8.1:58413775-58413794 MS.gene068940:CDS 35.0%
TCTATTAAGGATGGTATGAG+AGG + chr8.1:58412315-58412334 MS.gene068940:CDS 35.0%
TGATCTTAGGCTTCATGATT+TGG + chr8.1:58413806-58413825 MS.gene068940:CDS 35.0%
TTGCTCATTGAATTCCTTGT+GGG - chr8.1:58412295-58412314 None:intergenic 35.0%
TTTGACCGACGTAAAATACT+CGG - chr8.1:58413194-58413213 None:intergenic 35.0%
TTTGTAGGTTTATTTGTGGC+AGG + chr8.1:58413410-58413429 MS.gene068940:intron 35.0%
TTTGTGTTCAAACCTGAAGA+TGG - chr8.1:58412128-58412147 None:intergenic 35.0%
! ACTAACCCTTTTAATGGAAG+TGG + chr8.1:58413756-58413775 MS.gene068940:CDS 35.0%
! CTTTTCCGAGTATTTTACGT+CGG + chr8.1:58413186-58413205 MS.gene068940:intron 35.0%
!! GTTGGAAGTGCTTAATTTGT+AGG + chr8.1:58413395-58413414 MS.gene068940:intron 35.0%
!! TTCAAAAGCACCTTCAATTG+AGG + chr8.1:58413433-58413452 MS.gene068940:CDS 35.0%
!!! ACATTTTAGGATGCAAATGG+AGG + chr8.1:58412932-58412951 MS.gene068940:intron 35.0%
!!! GCAACATTTTAGGATGCAAA+TGG + chr8.1:58412929-58412948 MS.gene068940:intron 35.0%
AAACCTGAAGATGGCTCTTA+AGG - chr8.1:58412119-58412138 None:intergenic 40.0%
AAAGCTTATCACACACTTGC+AGG + chr8.1:58413495-58413514 MS.gene068940:CDS 40.0%
AGCTGCAGCTACAAATTTCA+AGG + chr8.1:58413530-58413549 MS.gene068940:CDS 40.0%
AGGAGAAGATGAGTACTATC+AGG - chr8.1:58411741-58411760 None:intergenic 40.0%
ATCATAGTTGAGGGCTTCTT+TGG - chr8.1:58411585-58411604 None:intergenic 40.0%
ATGGCTCTTAAGGTGGTAAA+GGG - chr8.1:58412109-58412128 None:intergenic 40.0%
CCACTCCAAAAACAATCATG+AGG + chr8.1:58412061-58412080 MS.gene068940:CDS 40.0%
GCTGCAGCTACAAATTTCAA+GGG + chr8.1:58413531-58413550 MS.gene068940:CDS 40.0%
GTTATTCCAAGCTTCACAGA+TGG - chr8.1:58413960-58413979 None:intergenic 40.0%
TGCTCATTGAATTCCTTGTG+GGG - chr8.1:58412294-58412313 None:intergenic 40.0%
TTTGATCCATCTGTGAAGCT+TGG + chr8.1:58413951-58413970 MS.gene068940:CDS 40.0%
! AAAGCGTTCATGAAGTTCGA+CGG - chr8.1:58411691-58411710 None:intergenic 40.0%
! AAGGGTATGAATGGAACTCA+AGG + chr8.1:58413549-58413568 MS.gene068940:CDS 40.0%
!!! CCTCATGATTGTTTTTGGAG+TGG - chr8.1:58412064-58412083 None:intergenic 40.0%
!!! TGTTGGTGGTGCAACATTTT+AGG + chr8.1:58412919-58412938 MS.gene068940:intron 40.0%
AAGACTGCATGAACAGCTAG+AGG + chr8.1:58412962-58412981 MS.gene068940:CDS 45.0%
AGAGTCACCTTGAACACACA+TGG - chr8.1:58411625-58411644 None:intergenic 45.0%
ATAGTTGAGGGCTTCTTTGG+AGG - chr8.1:58411582-58411601 None:intergenic 45.0%
ATGAGGGTTATGGGTGTGAA+GGG + chr8.1:58412078-58412097 MS.gene068940:CDS 45.0%
ATGATCAATGCTGTTGCAGC+TGG + chr8.1:58413987-58414006 MS.gene068940:CDS 45.0%
ATGTGTGTTCAAGGTGACTC+TGG + chr8.1:58411624-58411643 MS.gene068940:CDS 45.0%
CAATTGAGGATTGAGGCTCA+AGG + chr8.1:58413447-58413466 MS.gene068940:CDS 45.0%
CACACTTGCAGGTGAAAACA+TGG + chr8.1:58413506-58413525 MS.gene068940:CDS 45.0%
GAGCCTCAATCCTCAATTGA+AGG - chr8.1:58413446-58413465 None:intergenic 45.0%
GAGTCACCTTGAACACACAT+GGG - chr8.1:58411624-58411643 None:intergenic 45.0%
GATAGACCCATGTGTGTTCA+AGG + chr8.1:58411615-58411634 MS.gene068940:CDS 45.0%
GATGGCTCTTAAGGTGGTAA+AGG - chr8.1:58412110-58412129 None:intergenic 45.0%
GCACCTTCAATTGAGGATTG+AGG + chr8.1:58413440-58413459 MS.gene068940:CDS 45.0%
GGTAGAAGCTCATCACCATT+TGG + chr8.1:58414008-58414027 MS.gene068940:CDS 45.0%
TAGTTGAGGGCTTCTTTGGA+GGG - chr8.1:58411581-58411600 None:intergenic 45.0%
TCACCACTGATCCAAAACCT+CGG + chr8.1:58411655-58411674 MS.gene068940:CDS 45.0%
TGTGGTGCAACTGCAAATGT+TGG + chr8.1:58413377-58413396 MS.gene068940:intron 45.0%
!! GCTGCAACAGCATTGATCAT+AGG - chr8.1:58413987-58414006 None:intergenic 45.0%
ATAGAGAGGCCAACCCTTGA+TGG + chr8.1:58413678-58413697 MS.gene068940:CDS 50.0%
ATCCAAAACCTCGGCTTCGA+TGG + chr8.1:58411664-58411683 MS.gene068940:CDS 50.0%
CATGAGGGTTATGGGTGTGA+AGG + chr8.1:58412077-58412096 MS.gene068940:CDS 50.0%
CCACCTTAAGAGCCATCTTC+AGG + chr8.1:58412113-58412132 MS.gene068940:CDS 50.0%
CCTGAAGATGGCTCTTAAGG+TGG - chr8.1:58412116-58412135 None:intergenic 50.0%
GTTGATGCTGTTGCTCAGCT+TGG + chr8.1:58411711-58411730 MS.gene068940:CDS 50.0%
TTGGATCAGTGGTGAGGACA+AGG - chr8.1:58411650-58411669 None:intergenic 50.0%
!!! AAGCCGAGGTTTTGGATCAG+TGG - chr8.1:58411661-58411680 None:intergenic 50.0%
!!! GAGGTTTTGGATCAGTGGTG+AGG - chr8.1:58411656-58411675 None:intergenic 50.0%
GATGCTGTTGCTCAGCTTGG+AGG + chr8.1:58411714-58411733 MS.gene068940:CDS 55.0%
GCGACCAGTCATAGAAGACG+AGG - chr8.1:58412484-58412503 None:intergenic 55.0%
GCTTGGAAAGCAACCCCACA+AGG + chr8.1:58412278-58412297 MS.gene068940:CDS 55.0%
GGCACCTCGTCTTCTATGAC+TGG + chr8.1:58412477-58412496 MS.gene068940:CDS 55.0%
! GTCCATCGAAGCCGAGGTTT+TGG - chr8.1:58411669-58411688 None:intergenic 55.0%
TCGACGGTCCATCGAAGCCG+AGG - chr8.1:58411675-58411694 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 58411564 58414034 58411564 ID=MS.gene068940
chr8.1 mRNA 58411564 58414034 58411564 ID=MS.gene068940.t1;Parent=MS.gene068940
chr8.1 exon 58411564 58411744 58411564 ID=MS.gene068940.t1.exon1;Parent=MS.gene068940.t1
chr8.1 CDS 58411564 58411744 58411564 ID=cds.MS.gene068940.t1;Parent=MS.gene068940.t1
chr8.1 exon 58412058 58412134 58412058 ID=MS.gene068940.t1.exon2;Parent=MS.gene068940.t1
chr8.1 CDS 58412058 58412134 58412058 ID=cds.MS.gene068940.t1;Parent=MS.gene068940.t1
chr8.1 exon 58412270 58412336 58412270 ID=MS.gene068940.t1.exon3;Parent=MS.gene068940.t1
chr8.1 CDS 58412270 58412336 58412270 ID=cds.MS.gene068940.t1;Parent=MS.gene068940.t1
chr8.1 exon 58412448 58412514 58412448 ID=MS.gene068940.t1.exon4;Parent=MS.gene068940.t1
chr8.1 CDS 58412448 58412514 58412448 ID=cds.MS.gene068940.t1;Parent=MS.gene068940.t1
chr8.1 exon 58412941 58412983 58412941 ID=MS.gene068940.t1.exon5;Parent=MS.gene068940.t1
chr8.1 CDS 58412941 58412983 58412941 ID=cds.MS.gene068940.t1;Parent=MS.gene068940.t1
chr8.1 exon 58413432 58414034 58413432 ID=MS.gene068940.t1.exon6;Parent=MS.gene068940.t1
chr8.1 CDS 58413432 58414034 58413432 ID=cds.MS.gene068940.t1;Parent=MS.gene068940.t1
Gene Sequence

>MS.gene068940

ATGGAAAGAATGTTCCCTCCAAAGAAGCCCTCAACTATGATGAATTCTCATGATAGACCCATGTGTGTTCAAGGTGACTCTGGCCTTGTCCTCACCACTGATCCAAAACCTCGGCTTCGATGGACCGTCGAACTTCATGAACGCTTTGTTGATGCTGTTGCTCAGCTTGGAGGACCTGATAGTACTCATCTTCTCCTTTTATTCTTTTCTTTCTTTTGGTGCAAACTACATAACTAGAAGGAAAAAAAAACAAGTTTTTTTTTTTTTGAAGAATATAACTAGAAAAAAATGAAATTATGAAACTCTTCTTTAATTTTATCACTAAATTTTGTGATGAGTAGCCCTCAAAAAACAAAAACAAAAAATTGTGATGAGTGAATATAAATATAATTTATTTATTAGTCTTTAATTATATATTTTTTTTCTAGTAATATAATTTGCAACTTTTTTTCTAGTAATTTAATTTTTTTTTCCTCTTGTGTGTTAATTTGTAGAAGCCACTCCAAAAACAATCATGAGGGTTATGGGTGTGAAGGGTCTTACCCTTTACCACCTTAAGAGCCATCTTCAGGTTTGAACACAAACTATATATTATTTTCTAAATTTCTTTACCTCATTTAGGTTCACTTTTGTGACACTATCAATTTTCTTTCTTTATACCTTTCCCTTTTAAGTTCTTTGATTTTATTTTCTTTTGCATTAATAGAAATTTAGGCTTGGAAAGCAACCCCACAAGGAATTCAATGAGCAATCTATTAAGGATGGTATGAGAGGTGACATATAACACTTTAATGTTCCTTAAGAATTTTTATTTTCTAAGCTTTTATCTCTTTTTACACTTTGTTGAATTCAGATGTATTATAACACTTTTGTTTTCATGACAGTTTCGGCTTTTGAACTGCAAAGAAATACTGGCACCTCGTCTTCTATGACTGGTCGCAACATGAATGAGTATGTTAATAATTACCTTTTGATTCTTAATTTATTTATTCAAACCCTCAACCACAAGTAAACTAATATTGAAAAGTATAGTTTCTTCCCTCTTCTATATACTTTTAGTCTTTTATTACTTTTTATTCTTTTTGTTTAGAGAATTTTTTCTTTAATTTGAATTTTTCTAGATGAATTGCTACATGAATCTATACTTAGTGCCTGCATATAATAACTACTAACTAATGACCTCAACATGTATTTTCCTGCATTTTAATTTAATTTTTTTTTAATTGTGCTCACAATAAAAAATTTTACGATTTGGATGCATTAATCTAGCTCATATTATAATGAGAAACTTTGGTTGATGTTTTTGTTGTACTGTGTTTTGTATGTGCCATTGTATGTAACCGTAACTAAAACTTTGTTGGTGGTGCAACATTTTAGGATGCAAATGGAGGTGCACAGAAGACTGCATGAACAGCTAGAGGTATACACAGCTACAAGTATCTAATAGTATATAGAGCTAGTGTTTTATGGCCACAAGATCATATGTATAGTGTTCAGGCACACGTACAAAGAGACAATTTTTTTTAATAATAAAAAATTAAATTAAAACAAAGTTAGTTTCTAATTTCTAGGAGTTAACAACACTAAAATTAAATAACAATACTATTTTTCAATTAAATTAACTTTTCCGAGTATTTTACGTCGGTCAAATAACAAAGTGATTGAGTTGGTTTGTAAACTTCACTAAGGTGAAATTGTTTTGAAGAAATCCGAGTTCAAATCTTTCTTGAAACAACTTTTTAATAAACTTTATTTACCTTCTGATTGAACTATAGATTACTAAAATTTCTCTCTAGGATTAAGACAAAAAATATGTGGTGCAACTGCAAATGTTGGAAGTGCTTAATTTGTAGGTTTATTTGTGGCAGGTTCAAAAGCACCTTCAATTGAGGATTGAGGCTCAAGGAAAATACATGCAAAGTATATTGGAGAAAGCTTATCACACACTTGCAGGTGAAAACATGGCAGCTGCAGCTACAAATTTCAAGGGTATGAATGGAACTCAAGGCATCCCTGACATGAAAGAATTTGTTTCTCCTCTAAATAATTTTCCTCATTTTCAAGATCTTAACATTTGTGGTAGTGATGATGAACAACTTGATCTTCATCAAAACATAGAGAGGCCAACCCTTGATGGAATTATAATGCCTAATAATAATAATGATGAAAATTTGTTTGTTGGAAAGAAAAGGACTAACCCTTTTAATGGAAGTGGAAAGAGTCCAATAATTTGGAATGATAATGATCTTAGGCTTCATGATTTGGGAACAACTTCATCATGCATTAGTCCTGATGATGTTCCTAATTTCAAAGGTGAATCATTGGATAGATGTGATGAAAGTGATTCTATTGTTGATATTTATGACACAAAAGTGAGTGAGAAGAAATTTGATCCATCTGTGAAGCTTGGAATAACATTACCTATGATCAATGCTGTTGCAGCTGGTAGAAGCTCATCACCATTTGGTTGA
Protein sequence

>MS.gene068940.t1

MERMFPPKKPSTMMNSHDRPMCVQGDSGLVLTTDPKPRLRWTVELHERFVDAVAQLGGPDKATPKTIMRVMGVKGLTLYHLKSHLQKFRLGKQPHKEFNEQSIKDGMRVSAFELQRNTGTSSSMTGRNMNEMQMEVHRRLHEQLEVQKHLQLRIEAQGKYMQSILEKAYHTLAGENMAAAATNFKGMNGTQGIPDMKEFVSPLNNFPHFQDLNICGSDDEQLDLHQNIERPTLDGIIMPNNNNDENLFVGKKRTNPFNGSGKSPIIWNDNDLRLHDLGTTSSCISPDDVPNFKGESLDRCDESDSIVDIYDTKVSEKKFDPSVKLGITLPMINAVAAGRSSSPFG